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1. Introduction
Retail stores manage millions of items on a day to day basis to deliver to their customers. Point of Sales (POS) systems with barcodes were among the first technologies used to track products across the supply chain and in stores. Barcodes, as an identification technology, are not utilized at item-level but usually represent a group of products. Retailers need to scan products at pallet level at the point of receiving shipments, in inventories entrance and exit places, and at the POS to keep track of what is coming into and leaving stores [1]. With barcode systems, inventory inaccuracy is created because stores barcode scanning are not always performed at the right time and the right location. This inventory inaccuracy leads to a significant loss at retailers. Retailers needed to explore new ways of tracking their items to lower the inventory inaccuracy and prevent consequent losses. Radio Frequency Identification (RFID) technology appears to be the new technology solution that could improve the inventory record accuracy of stores for various items.
RFID technology applications have been recognized in many areas such as healthcare, finance, manufacturing, and retail. The share of the RFID market in retail is projected to be the largest of all sectors with about 34% by 2026, followed by transportation sector (25%), financial and security services (22%), and other industries such as healthcare and manufacturing at smaller portions [2]. RFID tags can store more information about each item at real time and can have individualized identification for items versus barcodes with a small data storage capacity that can only identify a group of items. RFID readers do not need to be on the line of sight to read RFID tags information which means items can be scanned more frequently and faster at any movement. These capabilities allow little mistakes in tracking records and largely eliminates inventory inaccuracy.
RFID technology’s benefits to retailers were identified early at the beginning of the 21st century. However, RFID’s applications in retail stores on a large scale took a while to be implemented. This paper reviews utilizing RFID, as an ideal solution to retail operations, since earlier this century and will cover a twenty-year horizon (2001–2020) divided into three equally long periods of 2001–2007, 2008–2014, and 2015–2020.
2. Early introduction and utilization of RFID (2001–2007)
In studies done earlier in this century, RFID was recognized as the next major identification technology to replace barcode systems in the retail industry [3, 4, 5, 6, 7]. Barcode systems have been used to track customer purchases, to manage inventory records, and to offer promotion and advertising in retail since 1970 [8]. Barcode tags, however, need to be on the line of readers to be read, a requirement that makes physical inventory counting a labor-intensive task and prevents stores from updating their inventory records frequently and on time. Therefore, with barcode systems, inventory inaccuracy is significant [9]. Inventory inaccuracy refers to the difference between inventory on record and the actual number of items on hand in stores. Inventory inaccuracy is caused by many factors such as transaction errors in the POS system, or shrinkage caused by possible employee/customer theft. Inventory inaccuracy means that stores may not be able to place inventory orders on time, resulting in out-of- stock conditions and consequently losing sales and hurting customer shopping experience. RFID technology, on the other hand, enhances product visibility in store operations and across the supply chain through the ease of reading RFID tag information and updating inventory records on a real-time basis.
Studies have investigated RFID benefits in different areas of retail operations, such as supply chain management, and show how inventory inaccuracy and consequently out-of-stock conditions are improved with the implementation of RFID across the supply chain [10, 11, 12]. Enhanced information visibility, provided by RFID in the supply chain, decreases uncertainties and lowers high inventory costs associated with the uncertainties [13, 14].
Many pilot studies during this period investigated and explored the applications of this technology at the pallet level, case level, and item levels in stores [1, 6, 12, 15, 16]. Bottani and Rizzi [1] conducted a case study in 2005 to analyze pallet and case-level implementation of RFID and enhanced visibility generated at the receiving gates and entrance doors from backstore to sales floors. They demonstrated that safety stock and inventory holdings can be significantly reduced and RFID benefits are broad, ranging from labor efficiencies to inventory management improvements. Cost–benefit analyses in this period showed that pallet-level implementations of RFID were more cost effective than case-level implementations.
Metro Group in Germany conducted some case studies in their stores to show item-level RFID applications can improve customers’ shopping experiences as well. They introduced some tools provided by RFID technology such as automatic checkout, smart carts that help customers navigate stores and find their items easier and faster, and smart dressing rooms that help customers find their desired apparel items more conveniently [6, 16]. They demonstrated that utilizing these tools significantly enhances customers’ shopping experience.
Walmart retail stores in the US were the first retailers that decided to mandate the implementation of RFID at pallet and case level across some of their supply chain in 2005. Walmart also did a pilot study with 24 stores over a period of around 6 months to measure how RFID can improve inventory management. They demonstrated out-of-stock conditions were significantly reduced with the implementation of RFID technology [12].
In Asia, two Singaporean fashion retailers piloted item-level RFID on their apparel stores and reported significant reduction in stocking time from hours to minutes that consequently increased the frequency of counting items with handheld readers and improved inventory accuracy [17].
3. Delays and reflection time on implementing RFID solutions (2008-2014)
Financial crises and the great recession that started in 2008 did not work to the advantage of retailers that were planning to implement RFID applications in their stores. During financial crises, businesses tend to adopt strategies that could help them sustain and survive by spending low and investing less. RFID technology implementation plans were mostly postponed or slowed down during the financial crises. However, this period was the best time to develop some foundations with respect to policies, regulations, and standardization of the technology.
Privacy issues raised by consumer protection agencies and standardization issues across different platforms put forward by case studies and pilot projects led to the development of some regulations and privacy policies by governments, institutions, and businesses. European Commission (EC) took an active role by funding many initiatives across Europe [18]. Initiatives such as Coordinating European efforts for promoting the European RFID value chain (CE RFID) [19] and Building Radio frequency Identification solutions for the Global Environment (BRIDGE) [20], conducted from 2006 to 2009, highlighted that wide implementation of RFID technology needs some regulations, standardizations, and privacy policies in place. For example, the BRIDGE project, coordinated by GS1, helped the industry to develop standardizations such as establishing a common format for the data stored on RFID tags, or the availability of possible frequency bands.
RFID tags can store identifiable consumers’ private data, which need to be protected. Therefore, EU members signed an agreement on the Privacy Impact Assessment framework in order to protect consumer privacy [21, 22, 23]. This agreement established some rules to be followed in the design of smart chips such as RFID tags to protect the privacy of consumers’ data. Consumers should be informed if RFID tags are utilized in stores. In addition, tags must be deactivated at the point of sales at no cost [24]. This framework was later expanded to cover some rules for smart meters as well. In the United States, lawsuits against RFID application patents as well as privacy issues in 2011–2013 were setbacks for large-scale implementations of the technology. The National Institute of Standards and Technology (NIST) in the US has helped to establish some guidelines to help retailers; however, most of the development of policies and standardizations have been initiated by corporations in the US.
In addition to developing policies and standardizations, businesses had more chances to identify and learn broader applications of RFID technology in retail. The focus of most of earlier pilot studies was how this new tracking technology helps manage inventories better in order to avoid out-of-stock conditions. However, the applications of the technology go beyond only inventory management and tracking items throughout the supply chain. As shown in the balanced scorecard developed in [25], RFID benefits extend to marketing and merchandising operations in retail as well (Figure 1).
Figure 1.
Balanced scorecard for RFID applications in retail.
In marketing, stores can monitor the behavior of consumers better when customers use tools such as smart carts or smart dressing rooms provided by RFID. Retailers can learn about consumers’ preferences and reflect that in the promotion and advertising offered to customers in real time while they shop. The available tools such as smart dressing rooms and smart carts also enhance customer shopping experience. Use of these tools enables customers to find their desired items more conveniently and faster, which eventually leads to higher customer satisfaction and increase in sales.
In merchandising, enhanced visibility on consumer behavior in stores provided by RFID can help retailers identify better assortments of products. In addition, an enhanced visibility means better shelf-replenishment; that is stores can reduce the shelf space since enhanced visibility on shelves allows retailers to replenish them as soon as they become emptied. Less shelf space leads to holding less number of items on shelves at any given time and consequently less inventory and capital held in stores, which allows retailers to invest in carrying more variety for products in stores.
There were also more studies during this period conducting cost–benefit analysis of the implementation of the technology. The fixed cost of implementation includes middleware, fixed antennas, sensors, and readers and the variable cost includes the cost of tags per item. The cost of tags can be added to the cost of each product but then the big question is who has to pay for that cost. Should the cost be transferred to consumers or should that be shared between retailers and manufacturers? The tag cost as the variable cost of utilizing the technology is huge and cost–benefit analysis studies have shown it to be a major barrier to the implementation of the technology during this period. Kasiri and Sharda [26] showed that the cost of tags in item-level implementation of RFID, as the variable cost, is cumbersome. Moreover, the cost can exceed the benefits in some cases depending on the extent to which stores implement RFID applications. The cost barrier was expected to weigh less as the cost of tags became lower over time.
4. Large-scale implementation of RFID (2015-2020)
Surveys of businesses show the implementation of RFID has picked up in this period. A survey of 60 retail executives throughout the United States and Europe showed about 73% of retailers had plans to implement RFID in 2016 [27]. Another survey in 2018 [28], however, showed that 92% of retailers in North America plan on implementing RFID, which is about a 20% increase from the 2016 results.
The cost of implementation has been decreasing over time, as expected, and at the same time retailers have learned how to partially implement the technology. Retailers realized that they do not need to fully implement the technology. In some cases, only tags and hand-held readers are used to add visibility of items in stores without many of the infrastructures such as antennas. Cloud services, on the other hand, have allowed retailers to eliminate some of the middleware cost as well. The leading European fashion stores C & A is one of many retailers that explored lower cost implementations with partial utilization of the technology [29].
RFID platforms can generate big data that are the records of tracking items throughout the supply chain and stores in real time. Businesses need big data and business analytics capabilities to fully utilize technologies [30]. The results of the analysis of such data can help retailers improve their processes such as shelf-replenishment process as well as variety and assortment planning that have been used in the same format for many years. A new timely replenishment process can result in better management of physical space, layouts, and lowering holding costs in stores. Furthermore, a better variety and assortment planning means fulfilling customers’ expectations and eliminating unpopular items that releases some capital and allow investment opportunities in other areas in retail.
Competing technologies to RFID have been developed and utilized over time as well. For example, Quick Response (QR) codes give retailers better ability to manage items compared to barcodes, Near-Field Communication (NFC) technology has some capabilities compared to UHF RFID, and most recently Amazon’s cameras increase product visibilities in stores for fast checkouts. In addition, retailers have different priorities in investing in new technologies and there is competition for dollars invested in various technologies by retailers. For instance, a retail chain can focus on improving inventory operations, but another retailer may be focused on improving marketing operations and customer shopping experience in stores by developing new apps that can assist customers make decisions during their shopping time in stores. In a different example, Gucci as an Italian luxury brand name does not suffer from inventory inaccuracy issues but their priority is customer shopping experience and they have utilized RFID tags to protect customers against counterfeiting across the supply chain until products reach their customers [31].
Omnichannel retail has been widely available during this period of time. Retailers’ customers can shop at any time, in any place, and via any shopping channel. Omnichannel retailing needs accurate inventories and enhanced product visibility more than any other time. Item-level RFID can, therefore, accommodate the needs of omnichannel retail more than other technologies available [32]. In addition, blockchain as the latest technology in retail can provide automatic exchange of product data carried by RFID tags between different partners across the supply chain. The blockchain in retail solutions are currently being studied in a consortium of large retailers such as Nike, Macy’s, and Dillard’s in an RFID lab at Auburn University [33].
The 3-S model (substitution, scale, and structure) introduced in [34] discussed and projected different phases of the adoption of RFID earlier when this technology was introduced. Later the 3-S model was adapted by [35] to describe the current stage of retailers’ implementation of RFID applications in retail (Figure 2). The substitution and scale stages are covering mostly what has been achieved during the three periods discussed in this paper. In the substitution phase, the RFID technology was utilized to replace the applications of barcode systems in tracking products. In the scale phase, the RFID applications are enabling retailers to manage their operations with more accuracy, efficiencies, and at a higher speed and scale. The structure phase, that is re-engineering processes and completely overhauling retail operations, is still underway. The RFID technology will enable retailers to accomplish things they could not imagine before and allows retailers to tap into completely new domains and applications.
Figure 2.
3-S model for RFID retail adoption.
5. Conclusion
Retailers have different needs based on the way they operate in stores. Some retailers must manage large inventories in stores. An enhanced visibility on their products help them improve inventory accuracies and avoid out-of-stock and increase their efficiencies. On the other hand, some retailers have small backstore inventories and every item they receive is put directly on their shelves and available to their customers. Inventory management is not their priority, but they need to focus more on customer shopping experience. Therefore, the enhanced visibility of items in stores is expected to promote retailers’ marketing operations. Depending on the way retailers operate and what their priorities are, retailers have to plan on implementing appropriate applications of RFID technology.
As discussed in this paper, RFID has been utilized broadly with various applications. As a revolutionary technology, RFID’s implementation can go beyond improving the current processes in retail operations. The current processes can innovatively change to debut completely new applications that are only possible with the enhanced visibility of items in real time. The ensuing big data that is derived from the visibility provided by RFID tags can be analyzed, leading to innovations in retail operations.
Implementing item-level RFID needs to be part of omnichannel strategy in the retail sector. With the wide-spread usage of online retail services such as Amazon, the competition in retail is tougher than ever before. In omnichannel services, retailers need to grant their customers easy access via different channels and make their products available in a variety of delivery services. The accessibility and fast delivery will not be possible with the level of visibility provided by barcode systems. Utilization of technology in retail is evolving quickly and RFID technology is the one that can definitely help retailers win in this overhaul.
\n',keywords:"RFID, RFID applications, RFID in retail, Retail sector, RFID in retail operations",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/74858.pdf",chapterXML:"https://mts.intechopen.com/source/xml/74858.xml",downloadPdfUrl:"/chapter/pdf-download/74858",previewPdfUrl:"/chapter/pdf-preview/74858",totalDownloads:344,totalViews:0,totalCrossrefCites:0,totalDimensionsCites:0,totalAltmetricsMentions:0,impactScore:0,impactScorePercentile:44,impactScoreQuartile:2,hasAltmetrics:0,dateSubmitted:"December 15th 2020",dateReviewed:"January 4th 2021",datePrePublished:"January 19th 2021",datePublished:"March 16th 2022",dateFinished:"January 18th 2021",readingETA:"0",abstract:"Radio Frequency Identification (RFID) technology is one of the latest product tracking technologies being utilized by retailers. Operations management improvements were among the first recognized applications of this technology earlier in the century. RFID applications in managing retail operations, such as inventory management and control, lead to significant benefits. However, RFID applications are not limited to operations management and go beyond the operations side to offer improvements in other areas in retail such as marketing and managing customers’ shopping experiences. In this research, we review the applications of RFID technology in retail since its introduction and how those applications have evolved over the last two decades to help retailers provide omnichannel services to their customers in the current market. We will demonstrate what strategic and tactical factors have helped retailers implement this technology and what factors have slowed down the process of adoption. We will also report on the latest status of the utilization of RFID in the retail sector.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/74858",risUrl:"/chapter/ris/74858",book:{id:"9878",slug:"electromagnetic-wave-propagation-for-industry-and-biomedical-applications"},signatures:"Narges Kasiri",authors:[{id:"333227",title:"Associate Prof.",name:"Narges Kasiri",middleName:null,surname:"Kasiri",fullName:"Narges Kasiri Kasiri",slug:"narges-kasiri-kasiri",email:"nkasiri@ithaca.edu",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Early introduction and utilization of RFID (2001–2007)",level:"1"},{id:"sec_3",title:"3. Delays and reflection time on implementing RFID solutions (2008-2014)",level:"1"},{id:"sec_4",title:"4. Large-scale implementation of RFID (2015-2020)",level:"1"},{id:"sec_5",title:"5. Conclusion",level:"1"}],chapterReferences:[{id:"B1",body:'Bottani, E., Rizzi, A. Economical assessment of the impact of RFID technology and EPC system on the Fast Moving Consumer Goods supply chain, International Journal of Production Economics, 2008, 112(2), 548-569'},{id:"B2",body:'RFID Forecasts, Players, and Opportunities 2016-2026. IDTechEx Annual RFID Report, 2016'},{id:"B3",body:'Kambil A. and Brooks J. D., Auto-ID across the value chain: From dramatic potential to greater efficiency and profit. Technical report, Auto-ID center, 2002'},{id:"B4",body:'Chappell G. , Durdan D., Gilbert G., Ginburg L., Smith J., and Tobolski J. Auto-ID on delivery: The value of Auto-ID technology in the retail supply chain. Technical report, Auto-ID center, 2003'},{id:"B5",body:'Loebbecke, C. Modernizing Retailing Worldwide at the Point of Sale. Management Information Systems Quarterly Executive (MISQE), 2004, 3(4), 177-187'},{id:"B6",body:'Loebbecke, C., Wolfram, G. Taking Content Integration to the POS: Enhancing Shopping Convenience in Metro\'s \'Future Store\'. Society of Information Management (SIM) Paper Award Competition, 2004'},{id:"B7",body:'Wang, Y. M., Wang, Y. S., & Yang, Y. F. Understanding the Determinants of RFID Adoption in the Manufacturing Industry. Technological Forecasting and Social Change, 2010, 77(5), 803-815'},{id:"B8",body:'Fraza, V. Ending inventory errors in 60 days. Modern Materials Handling, 2000, 55(3), A11'},{id:"B9",body:'DeHoratius, N., and Raman, A. Inventory record inaccuracy: An empirical analysis. Management Science, 2008, 54 (4), 627-641'},{id:"B10",body:'Atali, A., Lee, H., and ÖlpOzer, O. If the inventory manager knew: Value of RFID under imperfect inventory information. White paper, Stanford University, 2005'},{id:"B11",body:'Heese, H. S. Inventory record inaccuracy, double marginalization and RFID adoption. Production and Operations Management,2007, 16(5), 542-553'},{id:"B12",body:'Hardgrave, B. C., Langford, S., Waller, M., and Miller, R. Measuring the impact of RFID on out of stocks at Wal-Mart. MISQE, 2008, 7(4), 181-192'},{id:"B13",body:'Delen, D., Hardgrave, B. C., and Sharda, R. RFID for better supply-chain management through enhanced information visibility, Productions and Operations Management Journal, 2007, 16(5), 316-324'},{id:"B14",body:'Zhou, W. RFID and item-level information visibility. European Journal of Operational Research, 2009, 198(1), 252-258'},{id:"B15",body:'Sharma, A., Thomas, D., & Konsynski, B. R. Strategic and Institutional Perspectives in the Evaluation, Adoption and Early Integration of Radio Frequency Identification (RFID): An Empirical Investigation of Current and Potential Adopters. Proceedings of the 41st Annual Hawaii International Conference on System Sciences, Waikoloa, Big Island of Hawaii, HI, 2008'},{id:"B16",body:'Krafft, M., Mantrala, M. K. Retailing in the 21st Century: Current and future Trends. 2006, New York:Springer'},{id:"B17",body:'Swedberg C., Two Singapore Fashion Retailers Use RFID to Track Inventory, 2008, RFID Journal, https://www.rfidjournal.com/two-singapore-fashion-retailers-use-rfid-to-track-inventory, accessed December 2020'},{id:"B18",body:'Wolfram, G., Birgit G., and Peter G., eds. The RFID Roadmap: The Next Steps for Europe. 2008, Springer Science & Business Media'},{id:"B19",body:'CORDIS. CE RFID. Coordinating European efforts for promoting the European RFID value chain, 2008, http://cordis.europa.eu/project/rcn/80154_en.html (2008, accessed July 2020)'},{id:"B20",body:'CORDIS. BRIDGE. Building Radio frequency Identification solutions for the Global Environment. 2009, http://cordis.europa.eu/project/rcn/93609_en.html (2009, accessed 18 July 2020). John Edwards Europe Is Rolling Out RFID. http://www.rfidjournal.com/articles/view?10000/2 (2012, accessed July 2020)'},{id:"B21",body:'European Commission 2. Small chips with big potential: New EU recommendations make sure 21st century bar codes respect privacy. http://europa.eu/rapid/press-release_IP-09-740_en.htm?locale=en (2009, accessed November 2020)'},{id:"B22",body:'European Commission 3. Privacy and Data Protection Impact Assessment Framework for RFID Applications. http://cordis.europa.eu/fp7/ict/enet/documents/rfid-pia-framework-final.pdf. (2011, accessed 18 July 2020)'},{id:"B23",body:'European Commission 4. Digital privacy: EU-wide logo and “data protection impact assessments” aim to boost the use of RFID systems. http://europa.eu/rapid/press-release_IP-14-889_en.htm. (2014, accessed November 2020)'},{id:"B24",body:'European Commission 1. Commission launches public consultation on radio frequency ID tags. http://europa.eu/rapid/press-release_IP-06-289_en.htm?locale=en. (2006, accessed November 2020)'},{id:"B25",body:'Kasiri, N., Sharda, R., Hardgrave, B. C. A Balanced Scorecard for Item-Level RFID in the Retail Sector: A Delphi Study, European Journal of Information Systems, 2012, 21(3), 255-267'},{id:"B26",body:'Kasiri, N., & Sharda, R. Real options and system dynamics for information technology investment decisions: Application to RFID adoption in retail. ACM Transactions on Management Information Systems (TMIS), 2013, 4(3), 11'},{id:"B27",body:'Unger, R. & Sain, J. Kurt Salmon RFID in retail study, 2016, https://easyscan.dk/wp-content/uploads/2018/03/rfid-retail_study_-kurt-salmon.pdf, accessed 6 August 2020'},{id:"B28",body:'Sain J., Wong A. Transforming modern retail: Findings of the 2018 RFID in retail study, 2018, https://www.accenture.com/us-en/insights/strategy/rfid-retail, accessed December 2020'},{id:"B29",body:'Swedberg C., C&A Rolls Out RFID to All Its French Stores, RFID Journal, 2016, https://www.rfidjournal.com/ca-rolls-out-rfid-to-all-of-its-french-stores, accessed November 2020'},{id:"B30",body:'Lee, H. L. Big data and the innovation cycle. Production and Operations Management, 2018, 27(9), 1642-1646'},{id:"B31",body:'Rae H., Stop Thief! These New RFID Tags Could Help Luxury Clothing Brands Guard Against Theft, 2016, Bloomburg, https://www.forbes.com/sites/haniyarae/2016/08/11/rfid-brands-theft/?sh=27298d3a18de, accessed December 2020'},{id:"B32",body:'Kumar, A., & Ting, P. RFID & Analytics Driving Agility in Apparel Supply Chain, 2019, https://dspace.mit.edu/handle/1721.1/121286, accessed December 2020'},{id:"B33",body:'Graen M., CHIP: Chain Integration Project, 2020, Auburn RFID Lab White Paper'},{id:"B34",body:'Lee, H.L. Peering through a glass darkly, International Commerce Review, 2007, 7(1), 61-68'},{id:"B35",body:'Kasiri, N., Erickson, S., Wolfram, J., A Comparative Study of RFID Adoption Patterns in Retail. 2020, Under Review'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Narges Kasiri",address:"nkasiri@ithaca.edu",affiliation:'
School of Business, Ithaca College, Ithaca, NY, USA
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1. Introduction
The genus Teucrium L. (Lamiaceae) is a genus growing in mild climate zones, particularly in the Mediterranean Basin and Central Asia [1]. Limited number of in-depth scientific researches have been done so far on the phytochemistry and bioactivities [2] of Teucrium genus that is represented by herbs or shrubs, with tubular or campanulate calyx, 2-lipped or actinomorphic, 5-toothed, the teeth equal or the upper larger; corolla with one 5-lobed lip; tube without a ring of hairs inside, often included in the calyx; and nutlets smooth or reticulate [1].
Teucrium species have been used in phytopharmacology, helping to treat many diseases, including tuberculosis, gastrointestinal disorders, inflammations, rheumatism, and diabetes [3]. The widespread applications of Teucrium genus in the ethnomedicine of several countries [1] may be due to its richness in biocompounds used in in vivo and in vitro biological effects. Moreover, traditional health care systems based on plants and plant-derived products are highly popular and employed therapeutically [2] in Tunisia. The plant essence that contains several secondary metabolites is synthetized in all parts (leaves, flowers, stem, seeds, buds). The use of essential oils in industries are markedly increased, including the beverage, food, aromatherapy, cosmetics and personal care [4]. Moreover, the recent trend in the field of inflammation research is to search for alternative therapeutic agents from natural sources that are devoid of the adverse effects characteristic for conventional steroids or nonsteroidal anti-inflammatory drugs (NSAIDs). In this context, phytochemical studies and biochemical investigations on the mode of action of traditional complementary remedies are of utmost importance [2]. Acute kidney damage, a great public health problem, has been grown in the world. It’s a critical care syndrome and an abrupt loss in renal function [5], resulting in acute reduction of renal activity and up to 22% mortality of hospitalized patients. Acute kidney injury is estimated to occur in about 20–200 per million population in the community, 7–18% of patients in hospital, and approximately 50% of patients admitted to the intensive care unit (ICU) [6]. Lipopolysaccharide (Escherichia coli 055:B5) is one of the most important causes of sepsis and is involved in the pathogenesis of sepsis-associated acute kidney injury (SA-AKI), which may lead to “cytokine storm,” intensified oxidative stress, low blood pressure, renal hypoperfusion, and finally a gradual decline in renal function [7].
T. ramosissimum belongs to the Lamiaceae family, of the genus Teucrium is known as “Hchichet Belgacem” or “Hchichet Ben Salem” in the region of Gafsa in the southwest of Tunisia [8]. The specie is present in the South of Tunisia in particular in Djebel Orbata (Zannouch-Gafsa, Tunisia) and Bou Hedma Mount (Sidi Bouzid-Tunisia). It exists as a small sub-shrub, bushy, of silver gray, 8–15 cm tall. The stems are slender, erect and small. While, the leaves are white, with rounded limb with 7 deep crenellations. The inflorescence is pauciflor; the white calyx is 4 mm long, with long acute and sub-natural teeth [8]. Three sesquiterpenoids (teucmosin, 4α-hydroxy-homalomenol C, 1β,4β,7α-trihydroxy-8,9-eudesmene), five sesquiterpenoids (oplopanone, homalomenol C, oxo-T-cadinol, 1β,4β,6β-trihydroxyeudesmane, 1β,4β,7α-trihydroxyeudesmane) and two trinorsesquiterpenoids (4β-hydroxy-11,12,13-trinor-5-eudesmen-1,7-dione and 1β,4β-dihydroxy-11,12,13-trinor-8,9-eudesmen-7-one) were isolated from the ethanolic extracts of the aerial parts of Teucrium ramosissimum [9]. Teucrium ramosissimum is particularly present in the higher mounts of southern Tunisia. The ethnopharmacological uses of this specie in Tunisia are for treatment of inflammation. In fact, many people apply the powder of this species on the external inflamed area to reduce swelling and pain.
The present study provides a new insight into the organ architecture of Teucrium ramosissimum Desf. Biochemical compounds of T. ramosissimum leaves were detected using GC/MS apparatus. Histological analysis and enzyme levels showed that T. ramosissimum decreased LPS-mediated acute kidney injury by inhibiting tissues inflammation and reducing kidney tissue damage.
2. Materials and methods
2.1 Chemicals
T. ramosissimum essential oil solutions (100 mg/ml) were diluted in dimethyl sulfoxide for in vivo analysis. 5-Fluorouracil (5-FU) and LPS (Escherichia coli 055:B5) was obtained from Sigma-Aldrich Chemicals Co. (St. Louis, MO, USA).
2.2 Plant materials
Leaves of T. ramosissimum were collected from the mount of Orbata (Gafsa, Tunisia) during the Springer (2018). Essential oil was extracted by Clevenger apparatus. Characterization of phytochemicals by GC-MS analysis indicated the presence of mono- and sesquiterpenic compounds.
2.3 Analyses of oily fractions of T. ramosissimum with GC/MS
Oily fractions (diluted in 10% hexane) were analysed using GC/MS on a model 6890 gas chromatograph with an autosampler coupled with an Agilent 5973 Mass Selective Detector (Agilent Technologies, Palo Alto, CA, USA) with an electron impact ionization of 70 eV. A Phenomenex capillary column, ZB-5MSi (30 m × 250 μm i.d., 0.5 μm film thickness) (Agilent Technologies, Hewlett-Packard, CA, USA) at a temperature rising from 40 to 280 °C (5 °C/min). The carrier gas at a purity of 99.999% used for GC/MS analyses was helium at a flow rate of 0.7 ml/min, a scan time of 1 s and mass range m/z 50–550. The terpenic compounds were identified by matching their retention indices with those of the Wiley 09 NIST 2011 mass spectral library of the apparatus.
2.4 Protective effects of T. ramosissimum against LPS-induced renal inflammation
2.4.1 Experimental design
Both sexes of Swiss albino mice (48, 25 g weight) were divided into 8 groups (n=6) and maintained in plastic cages (polypropylene). Mice, provided from Pasteur Animal Laboratory (Tunisia, Ethic# LNSP/Pro 152012), were housed under animal conditions (25 ± 5°C; 45–55 % relative humidity; 12 h light/dark cycles with free access to water and food.
Group 1: Normal control, orally treated with saline;
Group 2: negative control, orally treated with 10 μg/ml LPS;
Group 3: orally treated with 20 μg/kg T. ramosissimum essential oil diluted in Tween 80 (2%);
Group 4: orally treated with 50 μg/kg T. ramosissimum essential oil diluted in Tween 80 (2%);
Group 5: comparator control, orally treated with 20 mg/ kg/day 5-FU;
Group 6: orally treated with the mixture of LPS and Teucrium essential oil (10 μg/ml and 20 μg/kg, respectively);
Group 7: orally treated with the mixture of LPS and Teucrium essential oil (10 μg/ml and 50 μg/kg, respectively);
Group 8: orally treated with the mixture of LPS and 5-FU (10 μg/ml and 20 mg/kg, respectively).
Animals received drugs for one week. In the groups 6, 7 and 8, mice were treated with Teucrium essential oil or 5-FU 1 hour before LPS administration.
At the 8th day, mice were sacrificed and blood samples were collected by glass capillary tubes for plasma biomarker analysis. Kidney tissues were collected and processed for microscopic analysis.
2.5 Statistical analyses
Statistical analyses of in vivo study were performed using GraphPad Prism 4.00 to compare different groups with each other we used a two-way analysis of variance (ANOVA), followed by Tukey’s multiple test. A value of P < 0.05 was considered statistically significant.
3. Results and discussion
3.1 Ethnobotanical and phytochemical analysis of T. ramosissimum
T. ramosissimum macromorphological features (nutlets, leaves, stems, flowers) are shown in Figure 1. The major phytocompounds were β-phellandrene, α-cadinol, T-Muurolol, α-bisabolol, camphor, endo-borneol, and epi-bicyclosesquiphellandrene (Figure 2).
Phytocompounds of T. ramosissimum essential oil isolated using GC/MS analysis.
3.2 Effects of T. ramosissimum on histological changes of cecum and serum biomarkers of the kidneys
LPS mediated a significantly (P<0.05) increase in the levels of plasma urea, creatinine and uric acid (Figure 3A). The pretreatment of mice with T. ramosissimum notably reduced the levels of plasma biomarkers in serum. Macroscopic features of kidney taken from treated and untreated groups are shown in Figure 3Bi. As depicted in Figure 3Bii, normal glomerular histoarchitectural and numerous tubules was seen in the kidney and a significant increase in the tubular injury scores after LPS treatment, otherwise, we noted fibrotic lesions, leukocyte infiltration indicative of the inflammation within different areas in the glomeruli, and renal tubules degeneration associated to tubular epithelium desquamation, while no observed damage was detected in mice from normal control group. After one week of treatment of mice with LPS, pronounced tubular necrosis and kidney fibrotic scaring was observed in kidney, which was significantly reversed by T. ramosissimum treatment that improves significantly the renal functions when it was given prior to LPS administration (Figure 3Biii).
Figure 3.
A. Creatinine (i), urea (ii) and UA (iii) levels in plasma. B. Macroscopical view of mice kidney (i), histopathological analysis (ii) of kidney sections (scale bar = 250 μm) observed by H&E staining and Kidney tubular injury score (iii) of treated groups. Green arrows- inflammatory cell infiltration; red arrows: hemorage; blue arrows: edema of the intertubular spaces; TRs: Teucrium ramosissimum; G: glomerulus; dG: degenerated glomerulus. (H&E staining, Magnification: a–e ×40; f ×10). Data represent mean ± SD, n=6; ** P<0.01 vs. LPS.
Free radicals- induced lipid peroxidation to be one of the major causes of cell membrane damage resulting in a series of pathological situations by causing acute and chronic renal injuries [10]. In fact, LPS is one of the most important causes of sepsis and is involved in the pathogenesis of SA-AKI, which may lead to “cytokine storm,” intensified oxidative stress, low blood pressure, renal hypoperfusion, and finally a gradual decline in renal function [7]. In this report, photomicrograph overview revealed the potent protective effect of T. ramosissimum that could effectively attenuate the pathological cecum and renal alteration in LPS-induced colorectal inflammation and acute kidney injury in mice model by reducing inflammation. These imply that T.ramosissimum pretreatment may attenuates the pathological features of LPS-induced inflammation revealed by Hematoxylin & Eosin (H&E) staining. The current results further support previous findings on the effect of LPS to mediate tubule and glomerulus degeneration.
T. ramosissimum given prior to LPS treatment induced decrease in serum biomarkers (urea, uric acid, creatinine). In this report, plasma creatinine and urea increased significantly in LPS-treated group, and this indicate diminished ability of the kidneys to filter these waste products from the blood and excrete them in the urine [11]. Additionally, this work demonstrated that LPS increased uric acid that mediated arteriolopathy and interstitial inflammation suggest mechanisms that would exacerbate or potentiate progressive renal functional decline after injury. This process involves accumulation of free radicals. Moreover, in the kidney, LPS binds to TLR4 proteins and mediates the proinflammatory cytokines release, and more precisely IL-1β and TNF-α [7] and induces the transcriptional factor, NF-kB, activation that regulates a variety of inflammatory gene expression [12]. Effectively, any compound able to modulate inflammation or inflammation-related processes can be thought of as a renal protective agent and/or a potential treatment tool for controlling renal damage [13].
T. ramosissimum is traditionally used for the treatment of many diseases (inflammation, gastric ulcer, cancer). Its extracts markedly enhance cell proliferation either with or without mitogen (lipopolysaccharide [LPS] or lectin) stimulation and contain potent components such as flavonoids that may be potentially useful for modulating immune cell functions in physiological and pathological conditions. Moreover, Teucrium extract exert different protective effects against ethanol-induced ulcerogenesis [14]. Likewise, this species acts as chemopreventive and chemosensitizing agent against two uterine sarcoma cell lines, MES-SA and P-gp-overexpressing MES-SA/Dx5 cells by a slight modulation of the cell cycle and its regulators, but also through a significant induction of apoptosis [15].
4. Conclusions
This report affirms that phytpharmacological effects of Teucrium essential oil extracted at the flowering stage may be related to its derived products identified by GC/MS apparatus. T. ramosissimum can restore LPS-induced renal damage by inhibiting inflammation. The increase of serum biomarkers, together with glomerular and tubular alterations clearly indicate renal dysfunction.
Acknowledgments
The authors extend their appreciation to The Ministry of Higher Education and Scientific Research of Tunisia.
Conflict of interest
The authors declare no conflict of interest.
\n',keywords:"Teucrium ramosissimum, essential oil, hairs, LPS, renal dysfunction",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/80673.pdf",chapterXML:"https://mts.intechopen.com/source/xml/80673.xml",downloadPdfUrl:"/chapter/pdf-download/80673",previewPdfUrl:"/chapter/pdf-preview/80673",totalDownloads:35,totalViews:0,totalCrossrefCites:0,dateSubmitted:"September 26th 2021",dateReviewed:"January 20th 2022",datePrePublished:"March 2nd 2022",datePublished:null,dateFinished:"March 1st 2022",readingETA:"0",abstract:"Teucrium esssential oil mediates an extensive spectrum of biological effects, including renal diseases. The aim of this research was to explore the ethnobotanical feature, biochemical composition and antiinflammatory potential of T. ramosissimum alone or prior the use of LPS-induced kidney damage. The essential oils were subjected to Gas chromatography-mass spectrometry (GC/MS) apparatus to detect biomolecules in T. ramosissimum. In vivo renal dysfunction induced by LPS was investigated using mouse model. Our data showed that oral treatment of animals with LPS highly increased level of serum biomarkers and induces renal dysfuntion, whereas, pre-treatment with T. ramosissimum mediated markedly histopatological changes of kidney architecture and ameliorates renal function. Dense cover of secretory structures in teucrium leaves may protect this specie. Overall, this study showed phytocompounds richness and interesting biological activities of Tunisian Teucrium ramosissimum. Essential oil of this specie T. ramossimum given prior to LPS exposure protected mice from renal inflammation.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/80673",risUrl:"/chapter/ris/80673",signatures:"Fatma Guesmi and Ahmed Landoulsi",book:{id:"11332",type:"book",title:"Essential Oils - Advances in Extractions and Biological Applications",subtitle:null,fullTitle:"Essential Oils - Advances in Extractions and Biological Applications",slug:null,publishedDate:null,bookSignature:"Ph.D. Mozaniel Santana De Oliveira and Dr. Eloisa Helena De Aguiar Andrade",coverURL:"https://cdn.intechopen.com/books/images_new/11332.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-80355-754-0",printIsbn:"978-1-80355-753-3",pdfIsbn:"978-1-80355-755-7",isAvailableForWebshopOrdering:!0,editors:[{id:"195290",title:"Ph.D.",name:"Mozaniel",middleName:null,surname:"Santana De Oliveira",slug:"mozaniel-santana-de-oliveira",fullName:"Mozaniel Santana De Oliveira"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Materials and methods",level:"1"},{id:"sec_2_2",title:"2.1 Chemicals",level:"2"},{id:"sec_3_2",title:"2.2 Plant materials",level:"2"},{id:"sec_4_2",title:"2.3 Analyses of oily fractions of T. ramosissimum with GC/MS",level:"2"},{id:"sec_5_2",title:"2.4 Protective effects of T. ramosissimum against LPS-induced renal inflammation",level:"2"},{id:"sec_5_3",title:"2.4.1 Experimental design",level:"3"},{id:"sec_7_2",title:"2.5 Statistical analyses",level:"2"},{id:"sec_9",title:"3. Results and discussion",level:"1"},{id:"sec_9_2",title:"3.1 Ethnobotanical and phytochemical analysis of T. ramosissimum",level:"2"},{id:"sec_10_2",title:"3.2 Effects of T. ramosissimum on histological changes of cecum and serum biomarkers of the kidneys",level:"2"},{id:"sec_12",title:"4. Conclusions",level:"1"},{id:"sec_13",title:"Acknowledgments",level:"1"},{id:"sec_16",title:"Conflict of interest",level:"1"}],chapterReferences:[{id:"B1",body:'Maccioni A, Falconieri D, Sanna C, Porcedda S, Piras A, Maxia A. Characterization of essential oils from different taxa belonging to the genus teucrium in Sardinia Island, Italy. Plants. 2021;10:1359. DOI: 10.3390/plants10071359'},{id:"B2",body:'Napagoda M, Gerstmeier J, Butschek H, De Soyza S, Pace S, Lorenz S, et al. The anti-inflammatory and antimicrobial potential of selected ethnomedicinal plants from Sri Lanka. Molecules. 2020;25:1894. DOI: 10.3390/molecules25081894'},{id:"B3",body:'Jarić S, Mitrović M, Pavlović P. Ethnobotanical features of teucrium species. In: Stanković M, editor. Teucrium Species: Biology and Applications. Cham: Springer; 2020. DOI: 10.1007/978-3-030-52159-2_5'},{id:"B4",body:'Irshad M, Ali Subhani M, Ali S, Hussain A. Biological Importance of Essential Oils. Rijeka: Intechopen; 2019. DOI: 10.5772/intechopen.87198'},{id:"B5",body:'Fortrie G, de Geus HRH, Betjes MGH. The aftermath of acute kidney injury: A narrative review of long-term mortality and renal function. Critical Care. 2019;23:24. DOI: 10.1186/s13054-019-2314-z'},{id:"B6",body:'Chawla LS, Bellomo R, Bihorac A, Goldstein SL, Siew ED, Bagshaw SM, et al. Acute kidney disease and renal recovery: Consensus report of the acute disease quality initiative (ADQI) 16 workgroup. Nature Reviews Nephrology. 2017;13:241-257'},{id:"B7",body:'Chen Y, Jin S, Teng X, Hu Z, Zhang Z, Qiu X, et al. Hydrogen sulfide attenuates LPS-induced acute kidney injury by inhibiting inflammation and oxidative stress. Oxidative Medicine and Cellular Longevity. 2018;2018:10. DOI: 10.1155/2018/6717212'},{id:"B8",body:'Ben Sghaier M, Louhichi T, Ammari Y. Ethnobotanical and phytopharmacological notes on teucrium ramosissimum L. Research Review Biosciences. 2017;12(3):132'},{id:"B9",body:'Henchiri H, Bodo B, Deville A, Dubost L, Zourgui L, Raies A, et al. Sesquiterpenoids from teucrium ramosissimum. Phytochemistry. 2009;70(11-12):135-1441'},{id:"B10",body:'Farzaei MH, Zangeneh MM, Goodarzi N, Zangeneh A. Stereological assessment of nephroprotective effects of trachyspermum ammi essential oil against carbon tetrachloride-induced nephrotoxicity in mice. International Journal of Morphology. 2018;36(2):750-757'},{id:"B11",body:'El-Demerdash F, Dewer Y, ElMazoudy RH, Attia AA. Kidney antioxidant status, biochemical parameters and histopathological changes induced by methomyl in CD-1 mice. Experimental Toxicology and Pathology. 2013;65:897-901'},{id:"B12",body:'Chunzhi G, Zunfeng L, Chengwei Q, Xiangmei B, Jingui Y. Hyperin protects against LPS-induced acute kidney injury by inhibiting TLR4 and NLRP3 signaling pathways. Oncotarget. 2016;7(50):82602-82608'},{id:"B13",body:'Avila-Carrasco L, García-Mayorga EA, Díaz-Avila DL, Garza-Veloz I, Martinez-Fierro ML, González-Mateo GT. Potential therapeutic effects of natural plant compounds in kidney disease. Molecules. 2021;26:6096. DOI: 10.3390/molecules26206096'},{id:"B14",body:'Ben Sghaier M, Harizi H, Louhichi T, Krifa M, Ghedira K, Chekir-Ghedira L. Anti-inflammatory and antiulcerogenic activities of leaf extracts and sesquiterpene from teucrium ramosissimum (Lamiaceae). Immunopharmacology and Immunotoxicology. 2011;33(4):656-662. DOI: 10.3109/08923973.2011.558903'},{id:"B15",body:'Lahmar A, Mathey A, Aires V, Elgueder D, Vejux A, Khlifi R, et al. Essential oils, pituranthos chloranthus and teucrium ramosissimum, chemosensitize resistant human uterine sarcoma MES-SA/Dx5 cells to doxorubicin by inducing apoptosis and targeting P-glycoprotein. Nutrients. 1719;2021:13. DOI: 10.3390/ nu13051719'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Fatma Guesmi",address:"guesmif6@gmail.com",affiliation:'
Faculty of Sciences of Bizerte, Laboratory of Risks Related to Environmental Stresses: Fight and Prevention, University of Carthage, Carthage, Tunisia
Faculty of Sciences of Bizerte, Laboratory of Risks Related to Environmental Stresses: Fight and Prevention, University of Carthage, Carthage, Tunisia
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IntechOpen Edited Volumes are integrated collections of chapters about particular topics that present new areas of research or novel syntheses of existing research and, as such, represent perspectives from various authors.
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Monographs is a self-contained work on a particular subject, or an aspect of it, written by one or more authors. Monographs usually have between 130 and 500 pages.
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IntechOpen Edited Volumes are integrated collections of chapters about particular topics that present new areas of research or novel syntheses of existing research and, as such, represent perspectives from various authors.
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Edited Volumes can be comprised of different types of chapters:
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RESEARCH CHAPTER – A research chapter reports the results of original research thus contributing to the body of knowledge in a particular area of study.
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REVIEW CHAPTER – A review chapter analyzes or examines research previously published by other scientists, rather than reporting new findings thus summarizing the current state of understanding on a topic.
\n\n
CASE STUDY – A case study involves an in-depth, and detailed examination of a particular topic.
\n\n
PERSPECTIVE CHAPTER – A perspective chapter offers a new point of view on existing problems, fundamental concepts, or common opinions on a specific topic. Perspective chapters can propose or support new hypotheses, or discuss the significance of newly achieved innovations. Perspective chapters can focus on current advances and future directions on a topic and include both original data and personal opinion.
\n\n
INTRODUCTORY CHAPTER – An introductory chapter states the purpose and goals of the book. The introductory chapter is written by the Academic Editor.
\n\n
MONOGRAPHS
\n\n
Monographs is a self-contained work on a particular subject, or an aspect of it, written by one or more authors. Monographs usually have between 130 and 500 pages.
\n\n
TYPES OF MONOGRAPHS:
\n\n
Single or multiple author manuscript
\n\n
COMPACTS
\n\n
Compacts provide a mid-length publishing format that bridges the gap between journal articles, book chapters, and monographs, and cover content across all scientific disciplines.
\n\n
Compacts are the preferred publishing option for brief research reports on new topics, in-depth case studies, dissertations, or essays exploring new ideas, issues, or broader topics on the research subject. Compacts usually have between 50 and 130 pages.
\n\n
CONFERENCE PROCEEDINGS
\n\n
Collection of papers presented at conferences, workshops, symposiums, or scientific courses, published in book format
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Early studies are focusing on the miRNA profile as a biomarker in disease. As discovery of human miRNAs increased in the setting of disease, the research focus was gradually shifted towards miRNA therapeutic strategy for diagnostic and treatment of disease. Increasing evidences suggest that miRNAs are the next important class of antisense therapeutic molecules, which have significant advantage over antisense such as siRNAs because miRNAs are naturally occurring endogenous molecules. Aberrant alteration of the endogenous miRNAs has been linked to the development of certain diseases. Correcting these altered miRNAs by their mimics or inhibitors has been developed as potential therapeutic approaches. Some of the miRNA-based therapeutics are processed in preclinical and clinical trial for treatment hepatitis C, liver cancer, and other diseases. Currently, the major focus in the development of miRNA-based therapeutics is how to increase the miRNA stability and optimize delivery systems for specific disease with minimal off-target effect. This chapter will first overview the miRNA biogenesis, patho- and physiologic function, and regulation of miRNA molecules. Then, we discuss the miRNA-based potential therapeutic approaches and implication in disease.",book:{id:"6987",slug:"antisense-therapy",title:"Antisense Therapy",fullTitle:"Antisense Therapy"},signatures:"Andrew Walayat, Meizi Yang and DaLiao Xiao",authors:[{id:"188957",title:"Dr.",name:"DaLiao",middleName:null,surname:"Xiao",slug:"daliao-xiao",fullName:"DaLiao Xiao"},{id:"269866",title:"Ph.D. Student",name:"Andrew",middleName:null,surname:"Walayat",slug:"andrew-walayat",fullName:"Andrew Walayat"},{id:"283826",title:"Dr.",name:"Meizi",middleName:null,surname:"Yang",slug:"meizi-yang",fullName:"Meizi Yang"}]},{id:"32799",doi:"10.5772/33525",title:"GC3 Biology in Eukaryotes and Prokaryotes",slug:"gc3-biology-in-eukaryotes-and-prokaryotes",totalDownloads:1971,totalCrossrefCites:7,totalDimensionsCites:15,abstract:null,book:{id:"1723",slug:"dna-methylation-from-genomics-to-technology",title:"DNA Methylation",fullTitle:"DNA Methylation - From Genomics to Technology"},signatures:"Eran Elhaik and Tatiana Tatarinova",authors:[{id:"95992",title:"Dr.",name:"Tatiana",middleName:"Valerievna",surname:"Tatarinova",slug:"tatiana-tatarinova",fullName:"Tatiana Tatarinova"},{id:"105570",title:"Dr.",name:"Eran",middleName:null,surname:"Elhaik",slug:"eran-elhaik",fullName:"Eran Elhaik"}]},{id:"65601",doi:"10.5772/intechopen.84455",title:"Epigenetic Modifications in Plants under Abiotic Stress",slug:"epigenetic-modifications-in-plants-under-abiotic-stress",totalDownloads:1552,totalCrossrefCites:12,totalDimensionsCites:15,abstract:"Plants face a plethora of biotic and abiotic stresses ranging from extreme temperatures to salinity, drought, nutritional deficiencies, chemical toxicity, and pathogen attacks. As a consequence, plants have acquired several sophisticated regulatory mechanisms that allow them to cope with such adverse conditions. Epigenetic regulation plays a key role in the mechanisms of plant response to the environment, without altering DNA sequences. Epigenetics refers to heritable alterations in chromatin architecture that do not involve changes in the underlying DNA sequence but alter gene expression through DNA methylation or histone modifications. The epigenetic regulation of the plant genome is a highly dynamic process that fine-tunes the expression of a pertinent set of genes under certain environmental or developmental conditions. Over the past two decades rapid advancements in the field of high throughput sequencing unveil epigenetic information at genome wide level in various plant species. In view of the adverse effects of global climatic change, utilizing epigenetic differences for developing improved crop varieties is of paramount importance.",book:{id:"7995",slug:"epigenetics",title:"Epigenetics",fullTitle:"Epigenetics"},signatures:"Garima Singroha and Pradeep Sharma",authors:[{id:"142882",title:"Dr.",name:"Pradeep",middleName:null,surname:"Sharma",slug:"pradeep-sharma",fullName:"Pradeep Sharma"},{id:"281215",title:"Dr.",name:"Garima",middleName:null,surname:"Singroha",slug:"garima-singroha",fullName:"Garima Singroha"}]},{id:"63488",doi:"10.5772/intechopen.80874",title:"Nontransformative Strategies for RNAi in Crop Protection",slug:"nontransformative-strategies-for-rnai-in-crop-protection",totalDownloads:2017,totalCrossrefCites:5,totalDimensionsCites:13,abstract:"RNAi in crop protection can be achieved not only by plant-incorporated protectants through plant transformation (transgenic) but also by nontransformative strategies such as formulations of sprayable dsRNAs used as direct control agents, resistance factor repressors, or developmental disruptors. Therefore, the RNAi-based biopesticides are expected to reach the market also in the form of nontransgenic strategies such as sprayable products, stem injection, root drenching, seed treatment, or powder/granule. While the delivery of dsRNA by transgenic expression is well established, it requires generations of crop plants and is costly, which may take years and delays for practical application, depending on the regulatory rules, plant transformability, genetic stability, and public acceptance of genetically modified crop species. DsRNA delivery as a nontransgenic approach was already published as a proof-of-concept work, so it is time to point out some directions on how the real potential for agriculture and crop protection is.",book:{id:"7331",slug:"modulating-gene-expression-abridging-the-rnai-and-crispr-cas9-technologies",title:"Modulating Gene Expression",fullTitle:"Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies"},signatures:"Deise Cagliari, Ericmar Avila dos Santos, Naymã Dias, Guy Smagghe\nand Moises Zotti",authors:null},{id:"64396",doi:"10.5772/intechopen.81847",title:"MiRNA-Based Therapeutics in Oncology, Realities, and Challenges",slug:"mirna-based-therapeutics-in-oncology-realities-and-challenges",totalDownloads:1733,totalCrossrefCites:4,totalDimensionsCites:11,abstract:"As master modulators of the human genome, miRNAs are involved in all cancer hallmarks, disrupting the normal function of their targets. By gaining or losing the function, miRNAs lead to the validation of tumor phenotype, its progression, and metastasis as well as to drug resistance. Increasing the evidence suggests that the modulation of miRNAs in cancer cells, by suppressing the oncogenic miRNAs (oncomiRs) and substituting the deficient tumor suppressive miRNAs (TS-miRNAs), could become a reliable tool for improving the cancer therapy. In this chapter, we will present an up-to-date overview of the role of miRNA-based therapeutics in oncology, highlighting their role in cancer management, how these therapies can be used, and which would be the future challenges related to miRNA-based therapies.",book:{id:"6987",slug:"antisense-therapy",title:"Antisense Therapy",fullTitle:"Antisense Therapy"},signatures:"Ovidiu Balacescu, Simona Visan, Oana Baldasici, Loredana Balacescu, Catalin Vlad and Patriciu Achimas-Cadariu",authors:[{id:"195763",title:"Ph.D.",name:"Ovidiu",middleName:null,surname:"Balacescu",slug:"ovidiu-balacescu",fullName:"Ovidiu Balacescu"},{id:"196758",title:"Dr.",name:"Loreadana",middleName:null,surname:"Balacescu",slug:"loreadana-balacescu",fullName:"Loreadana Balacescu"},{id:"196770",title:"Prof.",name:"Patriciu",middleName:null,surname:"Achimas",slug:"patriciu-achimas",fullName:"Patriciu Achimas"},{id:"269553",title:"MSc.",name:"Oana",middleName:null,surname:"Baldasici",slug:"oana-baldasici",fullName:"Oana Baldasici"},{id:"269554",title:"Dr.",name:"Simona",middleName:null,surname:"Visan",slug:"simona-visan",fullName:"Simona Visan"},{id:"279298",title:"Dr.",name:"Catalin",middleName:null,surname:"Vlad",slug:"catalin-vlad",fullName:"Catalin Vlad"}]}],mostDownloadedChaptersLast30Days:[{id:"69649",title:"Introductory Chapter: Gene Expression and Phenotypic Traits",slug:"introductory-chapter-gene-expression-and-phenotypic-traits",totalDownloads:1222,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"8992",slug:"gene-expression-and-phenotypic-traits",title:"Gene Expression and Phenotypic Traits",fullTitle:"Gene Expression and Phenotypic Traits"},signatures:"Yuan-Chuan Chen",authors:[{id:"185559",title:"Dr.",name:"Yuan-Chuan",middleName:null,surname:"Chen",slug:"yuan-chuan-chen",fullName:"Yuan-Chuan Chen"}]},{id:"66368",title:"Introductory Chapter: Gene Editing Technologies and Applications",slug:"introductory-chapter-gene-editing-technologies-and-applications",totalDownloads:1115,totalCrossrefCites:0,totalDimensionsCites:3,abstract:null,book:{id:"8891",slug:"gene-editing-technologies-and-applications",title:"Gene Editing",fullTitle:"Gene Editing - Technologies and Applications"},signatures:"Yuan-Chuan Chen",authors:[{id:"185559",title:"Dr.",name:"Yuan-Chuan",middleName:null,surname:"Chen",slug:"yuan-chuan-chen",fullName:"Yuan-Chuan Chen"}]},{id:"64290",title:"Strand Displacement Amplification for Multiplex Detection of Nucleic Acids",slug:"strand-displacement-amplification-for-multiplex-detection-of-nucleic-acids",totalDownloads:2140,totalCrossrefCites:1,totalDimensionsCites:2,abstract:"The identification of various targets such as bacteria, viruses, and other cells remains a prerequisite for point-of-care diagnostics and biotechnological applications. Nucleic acids, as encoding information for all forms of life, are excellent biomarkers for detecting pathogens, hereditary diseases, and cancers. To date, many techniques have been developed to detect nucleic acids. However, most of them are based on polymerase chain reaction (PCR) technology. These methods are sensitive and robust, but they require expensive instruments and trained personnel. DNA strand displacement amplification is carried out under isothermal conditions and therefore does not need expensive instruments. It is simple, fast, sensitive, specific, and inexpensive. In this chapter, we introduce the principles, methods, and updated applications of DNA strand displacement technology in the detection of infectious diseases. We also discuss how robust, sensitive, and specific nucleic acid detection could be obtained when combined with the novel CRISPR/Cas system.",book:{id:"7331",slug:"modulating-gene-expression-abridging-the-rnai-and-crispr-cas9-technologies",title:"Modulating Gene Expression",fullTitle:"Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies"},signatures:"Lingwen Zeng, Omar Mukama, Xuewen Lu, Shilin Cao and Donghai\nLin",authors:null},{id:"63557",title:"Molecular Identification of Genetically Modified Crops for Biosafety and Legitimacy of Transgenes",slug:"molecular-identification-of-genetically-modified-crops-for-biosafety-and-legitimacy-of-transgenes",totalDownloads:1949,totalCrossrefCites:2,totalDimensionsCites:5,abstract:"Crops undergo artificially DNA modifications for improvements are considered as genetically modified (GM) crops. These modifications could be in indigenous DNA or by introduction of foreign DNA as transgenes. There are 29 different crops and fruit trees in 42 countries, which have been successfully modified for various traits like herbicide tolerance, insect/pest resistance, disease resistance and quality improvement. GM crops are grown worldwide and its area is significantly increasing every year. Many countries have very strict rules and regulations for GM crops and are also a trade barrier in some situations. Hence, identification and testing of crops for GM contents is important for identity and legitimacy of transgene to simplify the international trade. Normally, molecular identification is performed at three different levels, i.e., DNA, RNA and protein, and each level has its own importance in testing about the nature and type of GM crops. In this chapter, current scenario of GM crops and different molecular testing tools are described in brief.",book:{id:"8891",slug:"gene-editing-technologies-and-applications",title:"Gene Editing",fullTitle:"Gene Editing - Technologies and Applications"},signatures:"Shahid Nazir, Muhammad Zaffar Iqbal and Sajid-ur-Rahman",authors:null},{id:"38872",title:"Repetitive DNA: A Tool to Explore Animal Genomes/Transcriptomes",slug:"repetitive-dna-a-tool-to-explore-animal-genomes-transcriptomes",totalDownloads:4634,totalCrossrefCites:3,totalDimensionsCites:7,abstract:null,book:{id:"2748",slug:"functional-genomics",title:"Functional Genomics",fullTitle:"Functional Genomics"},signatures:"Deepali Pathak and Sher Ali",authors:[{id:"33032",title:"Dr.",name:"Sher",middleName:null,surname:"Ali",slug:"sher-ali",fullName:"Sher Ali"},{id:"141455",title:"Dr.",name:"Deepali",middleName:null,surname:"Pathak",slug:"deepali-pathak",fullName:"Deepali Pathak"}]}],onlineFirstChaptersFilter:{topicId:"53",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:98,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:287,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:10,numberOfPublishedChapters:103,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:10,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"25",title:"Environmental Sciences",doi:"10.5772/intechopen.100362",issn:"2754-6713",scope:"
\r\n\tScientists have long researched to understand the environment and man’s place in it. The search for this knowledge grows in importance as rapid increases in population and economic development intensify humans’ stresses on ecosystems. Fortunately, rapid increases in multiple scientific areas are advancing our understanding of environmental sciences. Breakthroughs in computing, molecular biology, ecology, and sustainability science are enhancing our ability to utilize environmental sciences to address real-world problems. \r\n\tThe four topics of this book series - Pollution; Environmental Resilience and Management; Ecosystems and Biodiversity; and Water Science - will address important areas of advancement in the environmental sciences. They will represent an excellent initial grouping of published works on these critical topics.
",coverUrl:"https://cdn.intechopen.com/series/covers/25.jpg",latestPublicationDate:"April 13th, 2022",hasOnlineFirst:!1,numberOfPublishedBooks:1,editor:{id:"197485",title:"Dr.",name:"J. Kevin",middleName:null,surname:"Summers",slug:"j.-kevin-summers",fullName:"J. Kevin Summers",profilePictureURL:"https://mts.intechopen.com/storage/users/197485/images/system/197485.jpg",biography:"J. Kevin Summers is a Senior Research Ecologist at the Environmental Protection Agency’s (EPA) Gulf Ecosystem Measurement and Modeling Division. He is currently working with colleagues in the Sustainable and Healthy Communities Program to develop an index of community resilience to natural hazards, an index of human well-being that can be linked to changes in the ecosystem, social and economic services, and a community sustainability tool for communities with populations under 40,000. He leads research efforts for indicator and indices development. Dr. Summers is a systems ecologist and began his career at the EPA in 1989 and has worked in various programs and capacities. This includes leading the National Coastal Assessment in collaboration with the Office of Water which culminated in the award-winning National Coastal Condition Report series (four volumes between 2001 and 2012), and which integrates water quality, sediment quality, habitat, and biological data to assess the ecosystem condition of the United States estuaries. He was acting National Program Director for Ecology for the EPA between 2004 and 2006. He has authored approximately 150 peer-reviewed journal articles, book chapters, and reports and has received many awards for technical accomplishments from the EPA and from outside of the agency. Dr. Summers holds a BA in Zoology and Psychology, an MA in Ecology, and Ph.D. in Systems Ecology/Biology.",institutionString:null,institution:{name:"Environmental Protection Agency",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:6,paginationItems:[{id:"22",title:"Applied Intelligence",coverUrl:"https://cdn.intechopen.com/series_topics/covers/22.jpg",isOpenForSubmission:!0,editor:{id:"27170",title:"Prof.",name:"Carlos",middleName:"M.",surname:"Travieso-Gonzalez",slug:"carlos-travieso-gonzalez",fullName:"Carlos Travieso-Gonzalez",profilePictureURL:"https://mts.intechopen.com/storage/users/27170/images/system/27170.jpeg",biography:"Carlos M. Travieso-González received his MSc degree in Telecommunication Engineering at Polytechnic University of Catalonia (UPC), Spain in 1997, and his Ph.D. degree in 2002 at the University of Las Palmas de Gran Canaria (ULPGC-Spain). He is a full professor of signal processing and pattern recognition and is head of the Signals and Communications Department at ULPGC, teaching from 2001 on subjects on signal processing and learning theory. His research lines are biometrics, biomedical signals and images, data mining, classification system, signal and image processing, machine learning, and environmental intelligence. He has researched in 52 international and Spanish research projects, some of them as head researcher. He is co-author of 4 books, co-editor of 27 proceedings books, guest editor for 8 JCR-ISI international journals, and up to 24 book chapters. He has over 450 papers published in international journals and conferences (81 of them indexed on JCR – ISI - Web of Science). He has published seven patents in the Spanish Patent and Trademark Office. He has been a supervisor on 8 Ph.D. theses (11 more are under supervision), and 130 master theses. He is the founder of The IEEE IWOBI conference series and the president of its Steering Committee, as well as the founder of both the InnoEducaTIC and APPIS conference series. He is an evaluator of project proposals for the European Union (H2020), Medical Research Council (MRC, UK), Spanish Government (ANECA, Spain), Research National Agency (ANR, France), DAAD (Germany), Argentinian Government, and the Colombian Institutions. He has been a reviewer in different indexed international journals (<70) and conferences (<250) since 2001. He has been a member of the IASTED Technical Committee on Image Processing from 2007 and a member of the IASTED Technical Committee on Artificial Intelligence and Expert Systems from 2011. \n\nHe has held the general chair position for the following: ACM-APPIS (2020, 2021), IEEE-IWOBI (2019, 2020 and 2020), A PPIS (2018, 2019), IEEE-IWOBI (2014, 2015, 2017, 2018), InnoEducaTIC (2014, 2017), IEEE-INES (2013), NoLISP (2011), JRBP (2012), and IEEE-ICCST (2005)\n\nHe is an associate editor of the Computational Intelligence and Neuroscience Journal (Hindawi – Q2 JCR-ISI). He was vice dean from 2004 to 2010 in the Higher Technical School of Telecommunication Engineers at ULPGC and the vice dean of Graduate and Postgraduate Studies from March 2013 to November 2017. He won the “Catedra Telefonica” Awards in Modality of Knowledge Transfer, 2017, 2018, and 2019 editions, and awards in Modality of COVID Research in 2020.\n\nPublic References:\nResearcher ID http://www.researcherid.com/rid/N-5967-2014\nORCID https://orcid.org/0000-0002-4621-2768 \nScopus Author ID https://www.scopus.com/authid/detail.uri?authorId=6602376272\nScholar Google https://scholar.google.es/citations?user=G1ks9nIAAAAJ&hl=en \nResearchGate https://www.researchgate.net/profile/Carlos_Travieso",institutionString:null,institution:{name:"University of Las Palmas de Gran Canaria",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"23",title:"Computational Neuroscience",coverUrl:"https://cdn.intechopen.com/series_topics/covers/23.jpg",isOpenForSubmission:!0,editor:{id:"14004",title:"Dr.",name:"Magnus",middleName:null,surname:"Johnsson",slug:"magnus-johnsson",fullName:"Magnus Johnsson",profilePictureURL:"https://mts.intechopen.com/storage/users/14004/images/system/14004.png",biography:"Dr Magnus Johnsson is a cross-disciplinary scientist, lecturer, scientific editor and AI/machine learning consultant from Sweden. \n\nHe is currently at Malmö University in Sweden, but also held positions at Lund University in Sweden and at Moscow Engineering Physics Institute. \nHe holds editorial positions at several international scientific journals and has served as a scientific editor for books and special journal issues. \nHis research interests are wide and include, but are not limited to, autonomous systems, computer modeling, artificial neural networks, artificial intelligence, cognitive neuroscience, cognitive robotics, cognitive architectures, cognitive aids and the philosophy of mind. \n\nDr. Johnsson has experience from working in the industry and he has a keen interest in the application of neural networks and artificial intelligence to fields like industry, finance, and medicine. \n\nWeb page: www.magnusjohnsson.se",institutionString:null,institution:{name:"Malmö University",institutionURL:null,country:{name:"Sweden"}}},editorTwo:null,editorThree:null},{id:"24",title:"Computer Vision",coverUrl:"https://cdn.intechopen.com/series_topics/covers/24.jpg",isOpenForSubmission:!0,editor:{id:"294154",title:"Prof.",name:"George",middleName:null,surname:"Papakostas",slug:"george-papakostas",fullName:"George Papakostas",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002hYaGbQAK/Profile_Picture_1624519712088",biography:"George A. Papakostas has received a diploma in Electrical and Computer Engineering in 1999 and the M.Sc. and Ph.D. degrees in Electrical and Computer Engineering in 2002 and 2007, respectively, from the Democritus University of Thrace (DUTH), Greece. Dr. Papakostas serves as a Tenured Full Professor at the Department of Computer Science, International Hellenic University, Greece. Dr. Papakostas has 10 years of experience in large-scale systems design as a senior software engineer and technical manager, and 20 years of research experience in the field of Artificial Intelligence. Currently, he is the Head of the “Visual Computing” division of HUman-MAchines INteraction Laboratory (HUMAIN-Lab) and the Director of the MPhil program “Advanced Technologies in Informatics and Computers” hosted by the Department of Computer Science, International Hellenic University. He has (co)authored more than 150 publications in indexed journals, international conferences and book chapters, 1 book (in Greek), 3 edited books, and 5 journal special issues. His publications have more than 2100 citations with h-index 27 (GoogleScholar). His research interests include computer/machine vision, machine learning, pattern recognition, computational intelligence. \nDr. Papakostas served as a reviewer in numerous journals, as a program\ncommittee member in international conferences and he is a member of the IAENG, MIR Labs, EUCogIII, INSTICC and the Technical Chamber of Greece (TEE).",institutionString:null,institution:{name:"International Hellenic University",institutionURL:null,country:{name:"Greece"}}},editorTwo:null,editorThree:null},{id:"25",title:"Evolutionary Computation",coverUrl:"https://cdn.intechopen.com/series_topics/covers/25.jpg",isOpenForSubmission:!0,editor:{id:"136112",title:"Dr.",name:"Sebastian",middleName:null,surname:"Ventura Soto",slug:"sebastian-ventura-soto",fullName:"Sebastian Ventura Soto",profilePictureURL:"https://mts.intechopen.com/storage/users/136112/images/system/136112.png",biography:"Sebastian Ventura is a Spanish researcher, a full professor with the Department of Computer Science and Numerical Analysis, University of Córdoba. Dr Ventura also holds the positions of Affiliated Professor at Virginia Commonwealth University (Richmond, USA) and Distinguished Adjunct Professor at King Abdulaziz University (Jeddah, Saudi Arabia). Additionally, he is deputy director of the Andalusian Research Institute in Data Science and Computational Intelligence (DaSCI) and heads the Knowledge Discovery and Intelligent Systems Research Laboratory. He has published more than ten books and over 300 articles in journals and scientific conferences. Currently, his work has received over 18,000 citations according to Google Scholar, including more than 2200 citations in 2020. In the last five years, he has published more than 60 papers in international journals indexed in the JCR (around 70% of them belonging to first quartile journals) and he has edited some Springer books “Supervised Descriptive Pattern Mining” (2018), “Multiple Instance Learning - Foundations and Algorithms” (2016), and “Pattern Mining with Evolutionary Algorithms” (2016). He has also been involved in more than 20 research projects supported by the Spanish and Andalusian governments and the European Union. He currently belongs to the editorial board of PeerJ Computer Science, Information Fusion and Engineering Applications of Artificial Intelligence journals, being also associate editor of Applied Computational Intelligence and Soft Computing and IEEE Transactions on Cybernetics. Finally, he is editor-in-chief of Progress in Artificial Intelligence. He is a Senior Member of the IEEE Computer, the IEEE Computational Intelligence, and the IEEE Systems, Man, and Cybernetics Societies, and the Association of Computing Machinery (ACM). Finally, his main research interests include data science, computational intelligence, and their applications.",institutionString:null,institution:{name:"University of Córdoba",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"26",title:"Machine Learning and Data Mining",coverUrl:"https://cdn.intechopen.com/series_topics/covers/26.jpg",isOpenForSubmission:!0,editor:{id:"24555",title:"Dr.",name:"Marco Antonio",middleName:null,surname:"Aceves Fernandez",slug:"marco-antonio-aceves-fernandez",fullName:"Marco Antonio Aceves Fernandez",profilePictureURL:"https://mts.intechopen.com/storage/users/24555/images/system/24555.jpg",biography:"Dr. Marco Antonio Aceves Fernandez obtained his B.Sc. (Eng.) in Telematics from the Universidad de Colima, Mexico. He obtained both his M.Sc. and Ph.D. from the University of Liverpool, England, in the field of Intelligent Systems. He is a full professor at the Universidad Autonoma de Queretaro, Mexico, and a member of the National System of Researchers (SNI) since 2009. Dr. Aceves Fernandez has published more than 80 research papers as well as a number of book chapters and congress papers. He has contributed in more than 20 funded research projects, both academic and industrial, in the area of artificial intelligence, ranging from environmental, biomedical, automotive, aviation, consumer, and robotics to other applications. He is also a honorary president at the National Association of Embedded Systems (AMESE), a senior member of the IEEE, and a board member of many institutions. 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He is the president of the Travel Medicine Committee of the Pan-American Infectious Diseases Association (API), as well as the president of the Colombian Association of Infectious Diseases (ACIN). He is a member of the Committee on Tropical Medicine, Zoonoses, and Travel Medicine of ACIN. He is a vice-president of the Latin American Society for Travel Medicine (SLAMVI) and a Member of the Council of the International Society for Infectious Diseases (ISID). Since 2014, he has been recognized as a Senior Researcher, at the Ministry of Science of Colombia. He is a professor at the Faculty of Medicine of the Fundacion Universitaria Autonoma de las Americas, in Pereira, Risaralda, Colombia. He is an External Professor, Master in Research on Tropical Medicine and International Health, Universitat de Barcelona, Spain. He is also a professor at the Master in Clinical Epidemiology and Biostatistics, Universidad Científica del Sur, Lima, Peru. In 2021 he has been awarded the “Raul Isturiz Award” Medal of the API. Also, in 2021, he was awarded with the “Jose Felix Patiño” Asclepius Staff Medal of the Colombian Medical College, due to his scientific contributions to COVID-19 during the pandemic. He is currently the Editor in Chief of the journal Travel Medicine and Infectious Diseases. His Scopus H index is 47 (Google Scholar H index, 68).",institutionString:"Institución Universitaria Visión de las Américas, Colombia",institution:null},{id:"332819",title:"Dr.",name:"Chukwudi Michael",middleName:"Michael",surname:"Egbuche",slug:"chukwudi-michael-egbuche",fullName:"Chukwudi Michael Egbuche",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/332819/images/14624_n.jpg",biography:"I an Dr. Chukwudi Michael Egbuche. I am a Senior Lecturer in the Department of Parasitology and Entomology, Nnamdi Azikiwe University, Awka.",institutionString:null,institution:{name:"Nnamdi Azikiwe University",country:{name:"Nigeria"}}},{id:"284232",title:"Mr.",name:"Nikunj",middleName:"U",surname:"Tandel",slug:"nikunj-tandel",fullName:"Nikunj Tandel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/284232/images/8275_n.jpg",biography:'Mr. Nikunj Tandel has completed his Master\'s degree in Biotechnology from VIT University, India in the year of 2012. He is having 8 years of research experience especially in the field of malaria epidemiology, immunology, and nanoparticle-based drug delivery system against the infectious diseases, autoimmune disorders and cancer. He has worked for the NIH funded-International Center of Excellence in Malaria Research project "Center for the study of complex malaria in India (CSCMi)" in collaboration with New York University. The preliminary objectives of the study are to understand and develop the evidence-based tools and interventions for the control and prevention of malaria in different sites of the INDIA. Alongside, with the help of next-generation genomics study, the team has studied the antimalarial drug resistance in India. Further, he has extended his research in the development of Humanized mice for the study of liver-stage malaria and identification of molecular marker(s) for the Artemisinin resistance. At present, his research focuses on understanding the role of B cells in the activation of CD8+ T cells in malaria. 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Professor Derbel is presently working at the Clinique les Oliviers, Sousse, Tunisia. His hospital activities are mostly concerned with laparoscopic, colorectal, pancreatic, hepatobiliary, and gastric surgery. He is also very interested in hernia surgery and performs ventral hernia repairs and inguinal hernia repairs. He has been a member of the GREPA and Tunisian Hernia Society (THS). During his residency, he managed patients suffering from diabetic foot, and he was very interested in this pathology. For this reason, he decided to coordinate a book project dealing with the diabetic foot. Professor Derbel has published many articles in journals and collaborates intensively with IntechOpen Access Publisher as an editor.",institutionString:"Clinique les Oliviers",institution:null},{id:"300144",title:"Dr.",name:"Meriem",middleName:null,surname:"Braiki",slug:"meriem-braiki",fullName:"Meriem Braiki",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/300144/images/system/300144.jpg",biography:"Dr. Meriem Braiki is a specialist in pediatric surgeon from Tunisia. She was born in 1985. She received her medical degree from the University of Medicine at Sousse, Tunisia. She achieved her surgical residency training periods in Pediatric Surgery departments at University Hospitals in Monastir, Tunis and France.\r\nShe is currently working at the Pediatric surgery department, Sidi Bouzid Hospital, Tunisia. Her hospital activities are mostly concerned with laparoscopic, parietal, urological and digestive surgery. 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I am currently working as a Professor at the Department of Medical Parasitology at Dokuz Eylul University, Izmir, Turkey.\n\nI have given many lectures, presentations in different academic meetings. I have more than 60 articles in peer-reviewed journals, 18 book chapters, 1 book editorship.\n\nMy research interests are Echinococcus granulosus, Echinococcus multilocularis (diagnosis, life cycle, in vitro and in vivo cultivation), and Trichomonas vaginalis (diagnosis, PCR, and in vitro cultivation).",institutionString:"Dokuz Eylül University",institution:{name:"Dokuz Eylül University",country:{name:"Turkey"}}},{id:"71812",title:"Prof.",name:"Hanem Fathy",middleName:"Fathy",surname:"Khater",slug:"hanem-fathy-khater",fullName:"Hanem Fathy Khater",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/71812/images/1167_n.jpg",biography:"Prof. Khater is a Professor of Parasitology at Benha University, Egypt. She studied for her doctoral degree, at the Department of Entomology, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, USA. She has completed her Ph.D. degrees in Parasitology in Egypt, from where she got the award for “the best scientific Ph.D. dissertation”. She worked at the School of Biological Sciences, Bristol, England, the UK in controlling insects of medical and veterinary importance as a grant from Newton Mosharafa, the British Council. Her research is focused on searching of pesticides against mosquitoes, house flies, lice, green bottle fly, camel nasal botfly, soft and hard ticks, mites, and the diamondback moth as well as control of several parasites using safe and natural materials to avoid drug resistances and environmental contamination.",institutionString:null,institution:{name:"Banha University",country:{name:"Egypt"}}},{id:"99780",title:"Prof.",name:"Omolade",middleName:"Olayinka",surname:"Okwa",slug:"omolade-okwa",fullName:"Omolade Okwa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/99780/images/system/99780.jpg",biography:"Omolade Olayinka Okwa is presently a Professor of Parasitology at Lagos State University, Nigeria. She has a PhD in Parasitology (1997), an MSc in Cellular Parasitology (1992), and a BSc (Hons) Zoology (1990) all from the University of Ibadan, Nigeria. She teaches parasitology at the undergraduate and postgraduate levels. She was a recipient of a Commonwealth fellowship supported by British Council tenable at the Centre for Entomology and Parasitology (CAEP), Keele University, United Kingdom between 2004 and 2005. She was awarded an Honorary Visiting Research Fellow at the same university from 2005 to 2007. \nShe has been an external examiner to the Department of Veterinary Microbiology and Parasitology, University of Ibadan, MSc programme between 2010 and 2012. She is a member of the Nigerian Society of Experimental Biology (NISEB), Parasitology and Public Health Society of Nigeria (PPSN), Science Association of Nigeria (SAN), Zoological Society of Nigeria (ZSN), and is Vice Chairperson of the Organisation of Women in Science (OWSG), LASU chapter. She served as Head of Department of Zoology and Environmental Biology, Lagos State University from 2007 to 2010 and 2014 to 2016. She is a reviewer for several local and international journals such as Unilag Journal of Science, Libyan Journal of Medicine, Journal of Medicine and Medical Sciences, and Annual Research and Review in Science. \nShe has authored 45 scientific research publications in local and international journals, 8 scientific reviews, 4 books, and 3 book chapters, which includes the books “Malaria Parasites” and “Malaria” which are IntechOpen access publications.",institutionString:"Lagos State University",institution:{name:"Lagos State University",country:{name:"Nigeria"}}},{id:"273100",title:"Dr.",name:"Vijay",middleName:null,surname:"Gayam",slug:"vijay-gayam",fullName:"Vijay Gayam",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/273100/images/system/273100.jpeg",biography:"Dr. Vijay Bhaskar Reddy Gayam is currently practicing as an internist at Interfaith Medical Center in Brooklyn, New York, USA. He is also a Clinical Assistant Professor at the SUNY Downstate University Hospital and Adjunct Professor of Medicine at the American University of Antigua. He is a holder of an M.B.B.S. degree bestowed to him by Osmania Medical College and received his M.D. at Interfaith Medical Center. His career goals thus far have heavily focused on direct patient care, medical education, and clinical research. He currently serves in two leadership capacities; Assistant Program Director of Medicine at Interfaith Medical Center and as a Councilor for the American\r\nFederation for Medical Research. As a true academician and researcher, he has more than 50 papers indexed in international peer-reviewed journals. He has also presented numerous papers in multiple national and international scientific conferences. His areas of research interest include general internal medicine, gastroenterology and hepatology. He serves as an editor, editorial board member and reviewer for multiple international journals. His research on Hepatitis C has been very successful and has led to multiple research awards, including the 'Equity in Prevention and Treatment Award” from the New York Department of Health Viral Hepatitis Symposium (2018) and the 'Presidential Poster Award” awarded to him by the American College of Gastroenterology (2018). He was also awarded 'Outstanding Clinician in General Medicine” by Venus International Foundation for his extensive research expertise and services, perform over and above the standard expected in the advancement of healthcare, patient safety and quality of care.",institutionString:"Interfaith Medical Center",institution:{name:"Interfaith Medical Center",country:{name:"United States of America"}}},{id:"93517",title:"Dr.",name:"Clement",middleName:"Adebajo",surname:"Meseko",slug:"clement-meseko",fullName:"Clement Meseko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/93517/images/system/93517.jpg",biography:"Dr. Clement Meseko obtained DVM and PhD degree in Veterinary Medicine and Virology respectively. He has worked for over 20 years in both private and public sectors including the academia, contributing to knowledge and control of infectious disease. Through the application of epidemiological skill, classical and molecular virological skills, he investigates viruses of economic and public health importance for the mitigation of the negative impact on people, animal and the environment in the context of Onehealth. \r\nDr. Meseko’s field experience on animal and zoonotic diseases and pathogen dynamics at the human-animal interface over the years shaped his carrier in research and scientific inquiries. He has been part of the investigation of Highly Pathogenic Avian Influenza incursions in sub Saharan Africa and monitors swine Influenza (Pandemic influenza Virus) agro-ecology and potential for interspecies transmission. He has authored and reviewed a number of journal articles and book chapters.",institutionString:"National Veterinary Research Institute",institution:{name:"National Veterinary Research Institute",country:{name:"Nigeria"}}},{id:"158026",title:"Prof.",name:"Shailendra K.",middleName:null,surname:"Saxena",slug:"shailendra-k.-saxena",fullName:"Shailendra K. Saxena",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRET3QAO/Profile_Picture_2022-05-10T10:10:26.jpeg",biography:"Professor Dr. Shailendra K. Saxena is a vice dean and professor at King George's Medical University, Lucknow, India. His research interests involve understanding the molecular mechanisms of host defense during human viral infections and developing new predictive, preventive, and therapeutic strategies for them using Japanese encephalitis virus (JEV), HIV, and emerging viruses as a model via stem cell and cell culture technologies. His research work has been published in various high-impact factor journals (Science, PNAS, Nature Medicine) with a high number of citations. He has received many awards and honors in India and abroad including various Young Scientist Awards, BBSRC India Partnering Award, and Dr. JC Bose National Award of Department of Biotechnology, Min. of Science and Technology, Govt. of India. Dr. Saxena is a fellow of various international societies/academies including the Royal College of Pathologists, United Kingdom; Royal Society of Medicine, London; Royal Society of Biology, United Kingdom; Royal Society of Chemistry, London; and Academy of Translational Medicine Professionals, Austria. He was named a Global Leader in Science by The Scientist. He is also an international opinion leader/expert in vaccination for Japanese encephalitis by IPIC (UK).",institutionString:"King George's Medical University",institution:{name:"King George's Medical University",country:{name:"India"}}},{id:"94928",title:"Dr.",name:"Takuo",middleName:null,surname:"Mizukami",slug:"takuo-mizukami",fullName:"Takuo Mizukami",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94928/images/6402_n.jpg",biography:null,institutionString:null,institution:{name:"National Institute of Infectious Diseases",country:{name:"Japan"}}},{id:"233433",title:"Dr.",name:"Yulia",middleName:null,surname:"Desheva",slug:"yulia-desheva",fullName:"Yulia Desheva",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/233433/images/system/233433.png",biography:"Dr. Yulia Desheva is a leading researcher at the Institute of Experimental Medicine, St. Petersburg, Russia. She is a professor in the Stomatology Faculty, St. Petersburg State University. She has expertise in the development and evaluation of a wide range of live mucosal vaccines against influenza and bacterial complications. Her research interests include immunity against influenza and COVID-19 and the development of immunization schemes for high-risk individuals.",institutionString:'Federal State Budgetary Scientific Institution "Institute of Experimental Medicine"',institution:null},{id:"238958",title:"Mr.",name:"Atamjit",middleName:null,surname:"Singh",slug:"atamjit-singh",fullName:"Atamjit Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/238958/images/6575_n.jpg",biography:null,institutionString:null,institution:null},{id:"333753",title:"Dr.",name:"Rais",middleName:null,surname:"Ahmed",slug:"rais-ahmed",fullName:"Rais Ahmed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333753/images/20168_n.jpg",biography:null,institutionString:null,institution:null},{id:"252058",title:"M.Sc.",name:"Juan",middleName:null,surname:"Sulca",slug:"juan-sulca",fullName:"Juan Sulca",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252058/images/12834_n.jpg",biography:null,institutionString:null,institution:null},{id:"191392",title:"Dr.",name:"Marimuthu",middleName:null,surname:"Govindarajan",slug:"marimuthu-govindarajan",fullName:"Marimuthu Govindarajan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/191392/images/5828_n.jpg",biography:"Dr. M. 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His research interests include computer/machine vision, machine learning, pattern recognition, computational intelligence. \nDr. Papakostas served as a reviewer in numerous journals, as a program\ncommittee member in international conferences and he is a member of the IAENG, MIR Labs, EUCogIII, INSTICC and the Technical Chamber of Greece (TEE).",institutionString:null,institution:{name:"International Hellenic University",institutionURL:null,country:{name:"Greece"}}},editorTwo:null,editorThree:null,series:{id:"14",title:"Artificial Intelligence",doi:"10.5772/intechopen.79920",issn:"2633-1403"},editorialBoard:[{id:"1177",title:"Prof.",name:"Antonio",middleName:"J. 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