Carbon dots sizes and synthesis techniques.
New ways of synthesizing organic-based fluorescent carbon dots (CDs) are required in environmental application. This is crucial for mitigation and control of pollutants without increasing the risk of releasing byproduct pollutants as the case with non-organic (metallic) quantum based substrate. Notably, this study provides current research on sustainable synthesis of CDs and their applications through analytical concept of recent and advance techniques for efficient and optimized processes. New scrutinized methods of synthesis and applications of CDs are beneficial and essential to optimize the state-of-art practices. The value distilled in this study adds to the field of sustainable production and application of CDs.
- carbon dots
1.1 Origin of carbon dots
Carbon dots (CDs) are nanoparticles generated from organic/inorganic sources, was first discovered in 2004 when single-wall carbon nanotubes were electrophoretically purified . CDs can be classified as carbon nanomaterials that are less than 10 nm in size, they are the latest class of fluorescent nanoparticles . CDs have attracted the interest of researchers in diverse fields of science and technology such as; optoelectronics , environmental pollution and remediation , biosensor , bio-imaging and biomedical applications [6, 7].
CDs possess properties such as being dimensionless, durable, large surface area, enhanced porosity and stability, ease of being functionalized, fluorescence emission, biocompatibility and low toxicity [5, 8]. These properties of CDs can be applied to improve the environment and human health [4, 9, 10].
A toxic rival to the CDs is the popular semiconductor nanocrystals popularly known as quantum dots (QDs). The QDs are a type of semiconductor nanoparticles with diameter range from 1 to 10 nm . More so, QDs normally are made from semiconducting materials, especially iron and cadmium, which are highly toxic and expensive to acquire . Compared to QDs, CDs are considered best option with a high degree of biocompatibility, cost-effectiveness and non-toxic. It also serves as a suitable substitute to QDs in numerous areas of research such as bio-imaging, bio-sensing, pharmaceutical and fuel cells [13, 14].
Carbon dots (CDs) are suitable for the modification of electrode sensors. It combines fundamental aspects of biology, chemistry, and physical sciences, computer science and electrical engineering to meet various needs in a wide application field. Therefore, carbon as a sensor portrays several meanings, conditional upon what field the user subscribes [15, 16].
Over the years, various bulk materials and several processes and techniques have been developed and adopted by a wide range of researchers in the synthesis of CDs. These processes include the hydrothermal and microwave-assisted routes, heating, biogenic synthesis, thermal oxidation, ultra-sonification, subcritical water process (use of oil bath and salt bath), refluxing and chemical oxidation [17, 18, 19, 20, 21, 22, 23, 24, 25, 26].
Three important factors must be considered in synthesizing CDs which are control of size, uniformity of CDs in solvents, and mitigated aggregation . Wang and Hu  confirmed that CDs carbonaceous aggregation tends to form during carbonization but this can be prevented when synthesized by methods such as electrochemical synthesis, hydrothermal, or by pyrolysis method.
The application of biological and agro-waste to synthesize CDs have been advocated in numerous research such as; cooking oil waste , egg-white and egg-yolk , orange juice  as well as eggshells . Though it is advantageous to use waste biomaterials in the synthesis of CDs to avoid competition with essential food production , however, the downside of the application of biomass in the synthesis of CDs is lacking of essential purity and structural homogeneity to obtain homogenous fluorescent CDs for purposes of sensing minute concentrations of analytes [21, 33]. These had caused the application of clean materials to be used in the synthesis of homogeneous fluorescent CDs .
A competent carbon source for soluble CDs synthesis is needed to comply with the goals of green chemistry and not be in direct competition with essential food production and should be cheap to synthesize [34, 35, 36]. Research in the synthesis of CDs must consider low price of additives and less purification steps in case of using biomass as a precursor material.
Thus, the emphasis is necessary on the cost of producing typical CDs, not to be a replica of the currently observed situation with semiconductor QDs, with the high cost and potential environmental negative impact and yet to achieve its full potential in commercial applications [37, 38, 39].
2. Green and sustainable carbon dots
Carbon dots (CDs) have emerged to be attractive materials due to their excellent photoluminescence (PL) properties and wide surface areas, which are needed for sensitive and selective sensing of analytes . These qualities are owed to the characteristics of the carbon element at nano-dimension and five valence electrons to bind carbon atoms [31, 41]. The green and sustainable carbons dots refer to CDs that are synthesized from agro and biomaterials that can be readily available without depleting their sources .
CDs can be obtained from various source . These sources include plants and animal origins such as bamboo leaves, woods, green algae, sugar cane, mangosteen, carica papaya, saffron, gringko, neem gum, prawn shells, orange, cucumber and pineapple [32, 43, 44, 45]. Further interesting applications of CDs have been reported in diverse sectors of the environment and health fields of science and technology [3, 32].
For instance, Pattanayak and Nayak, in 2013  presented an eco-friendly synthesis of iron nanoparticles from various plants and spices extract. The synthesis of nanoparticles from plant parts (leaf) is essential since this will not require expensive processes that are involved mostly in biomaterial processing. Iravani et al.,  demonstrated a green synthesis of metal nanoparticle using plants (
Liu , reported a research work on one-step green synthesized fluorescent carbon nano-dots from bamboo leaves for copper (ll) ion detection and demonstrated the exploration of bamboo leaves as a carbon source. Carbon nano-dots were synthesized hydrothermally and a resultant high quantum yield quantum dots, with sensitive Cu2+ detection at a limit of detection as low as 115 nM on a dynamic range from 0.333 to 66.6 μM. The zeta potential of the pristine carbon quantum dots was measured at −4.78 mV which changes to +13.8 mV after treatment with positively charged polyethyleneimine (a water-soluble cationic polymer).
Wembo et al
Piyushi et al.,  cultivated
Till et al.,  synthesized CDs by microwave-assisted hydrothermal treatment of starch and Tris-acetate-EDTA. The process confirmed that nitrogen-doped CDs have emerged to be complementary to starch-derived CDs. Addition of nitrogen to CDs improved the yield of photoluminescence from 19% to 28%, making them promising luminescent materials for improving fluorescence of CDs. However, there is no added value in incurring additional chemicals during synthesis process of CDs. Starch is a better alternative to the use of nitrogen for synthesizing CDs. Till and colleagues observed the effect of nitrogen (N) additives, through the use of ethylenediaminetetraacetic acid (EDTA); tris (hydroxymethyl) aminomethane (Tris) and a combination of both (TAE-buffer) on the photophysical properties of CDs. Temperature (45 min at 230°C) plays an important role in the improved nitrogen-doped carbon structures .
Some researchers have adopted nitrogen for fluorescence and photoluminescence enhancement, but this approach has shown indistinct composition which required extensive purification steps. This, however, is environmentally not suitable and contravenes the concept of green chemistry since it involves many chemicals in the synthesis process .
2.1 Methods of carbon dots (CDs) synthesis
Synthesis methods of CDs can be divided into two major parts; top-down and bottom-up as in Figure 1. Top-down starts from cutting the carbon materials into carbon particles or cleavage of larger carbonaceous materials such as carbon nanotube by laser ablation, arc discharge, electrochemical and candle/natural gas burner soot, and recently the hydrothermal route.
The bottom-up route involves the use of molecules as support for localizing the growth of CDs by blocking aggregation during high-temperature treatment. However, this study explores the top-down process of CDs synthesis. As earlier stated the top-down approach concentrates on precursor carbonization that include microwave-assisted method, chemical oxidation, heating, and hydrothermal process .
2.1.1 Laser ablation
It is a process removal of material from solid or liquid by irradiating it with a laser beam [51, 52, 53, 54]. Material evaporates or sublimates when the laser flux is low and converted to plasma at high laser flux. Goncalves and colleagues  reported the synthesis of CDs from carbon targets immersed in deionized water by direct laser ablation (UV pulsed laser irradiation). CDs were optimized and synthesized after being functionalized with NH2– polyethylene-glycol (PEG200) and N-acetyl-l-cysteine (NAC). To produce particles in tens of nanometer range by laser ablation, the energy is controlled within the incidence area of the precursor [54, 55].
Yu et al.,  demonstrated the possibilities of relying on irradiating a toluene sample with a non-focused pulsed laser that is very different from the high powered laser irradiation employed in conventional ablation. This process by Yu and colleagues revealed an induced transformation of toluene into graphene sheaths, which subsequently produced fluorescent CDs. These nanoparticles can simply be functionalized using more than one molecule and stayed stable in an aqueous solution. It can also be applied to optical fiber devices through immobilization due to its stability in a specific optical nano-analytical sensor . However, the equipment to conduct laser ablation is quite expensive and it needs technically skilled personnel to operate.
2.1.2 Arc discharge soot
CDs were first discovered through this method accidentally when the separation of single-walled carbon nanotubes (SWNTs) were made using gel electrophoresis from carbon soot by arc discharge method. Carbon is formed when direct current arc voltage is applied in an inert gas across two graphite electrodes. The biggest challenge of this method is that it generates impurities that are difficult to purify .
2.1.3 Electrochemical method
Electrochemistry is another top-down approach used in synthesizing CDs, this process is facile and the product yield is normally high . CDs with a size of 6 to 8 nm and 2.8% to 52% can be obtained through exfoliation that utilizes graphite rods and Pt wire in ionic liquid or water solution.
The mechanism of the exfoliation was due to complex interplay of anodic oxidative cleavage of water and anionic intercalation from the ionic liquid using titanium cathode and spectrum pure graphite in the center of electrolyzer to yield pure blue fluorescent CDs without the urgency of complex purification [28, 58, 59, 60, 61, 62].
2.1.4 Candle and natural gas burner soot
Application of carbon soot in the synthesis of CDs have been reported by Tian et al., , the carbon source was from a carbon-processing reaction. Due to the simplicity to obtain the starting material, this method has been used widely by researchers. It also provides a new use for a complicated by-product. At the same time, it possesses disadvantages such as uncontrolled chemical surface, production of many byproducts that can harm human health with a broad dispersion .
2.1.5 Microwave synthesis
Wang et al.,  prepared CDs by microwave method. It proved reaction time can be shortened to 30–45 minutes with microwave-assisted technique. Similarly, Choi et al.,  made effective use of lysine as a precursor to synthesize CDs within 5 minutes in a home type of microwave oven and the CDs were soluble in water with deep blue photoluminescence at a high mass yield of 23.3%.
Compared with other methods, the microwave route is more convenient since the heating of the carbon precursor is rapidly achieved within few minutes. It also exhibits high quantum yield and provides a long fluorescence lifetime. The procedure of microwave synthesis is much easier compared to others as it only utilizes heating via irradiation technique .
2.1.6 Chemical oxidation
This method is mostly applied to produce CDs on an industrial scale. CDs can be obtained through oxidation treatment of carbon precursors by a strong oxidant. CDs from natural products have been researched and developed, by the synthesis of large scale CDs from human hair, coffee, and biomass by adding it into concentrated sulfuric acid and then heating at different temperatures. The time range is from hours to days . By varying the temperature of synthesizing CDs, the quality of CDs such as diameter and quantum yields can be controlled [67, 68, 69].
2.1.7 Hydrothermal synthesis
The hydrothermal route of synthesizing CDs is considered as environmental-friendly, low cost and involves few synthesis steps that are non-toxic [2, 70, 71, 72, 73, 74]. Musa et al explored the hydrothermal method at a temperature range between 75°C to 175°C where the researchers reacted the precursor in a sealed hydrothermal reactor that resulted into a high yield photoluminescent quantum yield at 34.9% .
As illustrated in Figure 2, tapioca was added to an aldehyde solvent (acetone + sodium hydroxide) to improve the mobility of glucose molecules in starch . The mixture underwent stages of reactions such as hydrolysis, adsorption, and gelatinization to particle disintegration simultaneously . The carbonization temperature breaks the bond between the starch, making it available for the reactive solvent which leads to hydrolysis to form disaccharide and gelatinized glucose. The disaccharides polymerized into polysaccharides and the gelatinized glucose yielded CDs for functional group characterizations .
Like all-natural products, starch undergoes seasonal changes and particularly the amylose/amylopectin ratio is influenced by plant species and area of plant cultivation, which could influence the CDs formation. Independently of seasonal changes and origin, a starch will provide CDs with highly reproducible photoluminescent properties .
Substances such as glucose, citric acid, banana juice, and protein are examples of many precursors used to prepare CDs by adopting the hydrothermal route of synthesis . Success has been reported in the synthesis of CDs through one-step hydrothermal carbonization using chitosan applied directly as a bioimaging agent . The hydrothermal method is promising in producing CDs and is suitable for industrial or large scale production . However, it is notable in 2010 where Zhang et al.  first reported a one-pot hydrothermal method to synthesize CDs from ascorbic acid in the presence of ethanol as solvent. Quantum yield and average particle sizes of their synthesized CDs were 6.79% and ~2 nm, respectively .
Several methods of synthesizing CDs has been explored in this section, to prevent the use of expensive precursor and energetic systems like in laser ablation. The hydrothermal synthesis route is being recommended as foremost for the sake of ecological sustainability . Chemical oxidation and exfoliations provide an inexpensive alternative although it employs large amounts of strong acid which is hazardous and undesirable .
The other methods of synthesizing CDs need multi-step experimental operations and some of them require post-treatments to improve their water solubility, stability, and luminescent. Besides, several other methods suffer from drawbacks such as they require complex process and high temperature, time-consuming, harsh synthetic materials, and are expensive. This causes their applicability to be limited . Several research successes proved that hydrothermal route to be a green method for the synthesis of CDs since the procedure produce soluble fluorescence CDs at reduce time and cost [2, 46, 79].
CDs derived from organic sources are excellent for the researcher and environment. Because the adoption of such material presents the choice to eliminate the need for metallic quantum dots, and any doping requirements, either through the use of sulfur (S) or nitrogen (N) agents. The use of metallic quantum dots and possible inclusion of S and N in enhancing their functionality contravenes the purpose of sustainable applications of nanomaterials in the modern field of nanotechnology .
Table 1 is a list of different synthesis techniques that have been attractive to researchers in recent years. The table provides a list of interesting techniques such as hydrothermal, microwave assisted, biogenic synthesis, thermal oxidation, ultrasonication, refluxing and chemical oxidation with excellent particle sizes [80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91]. The hydrothermal synthesis of CDs proves to be efficient and effective since it provides relatively smaller sizes of the nanoparticles as synthesized by Du et al.,  at 1.8 nm, when compared to other methods such as chemical oxidation by Thambiraj and Shankaran  at 4.1 nm, biogenic synthesis by Phadke et al.,  at 5–8 nm, and refluxing by Himaja et al.,  at ~50 nm.
2.2 Properties and characterization of fluorescent carbon dots (CDs)
One of the CDs properties is that it shows strong optical absorption in the UV region (200–800 nm) with a tail extending to the visible range, see Figure 3. CDs possess low toxicity with excellent photostability as compared to semi-conductor quantum dots [7, 23, 50, 71].
The uniqueness of CDs is the availability of wide surface area for trace detection of analytes and provision of adsorptive sites through the availability of heteroatomic carbon in nano-dimension along with photoluminescence emission. Based on past study, CDs is dependent on intensity and wavelength emission towards its excitation wavelength . This is due to the different sizes of particles and surface chemistry and/or different emissive traps on CDs’ surface. The wavelength dependence behaviour makes CDs possible to be applied in multi-colour imaging and adsorptive purposes. Vinci et al
|Colour||Interval of wavelength (nm)|
The colour of CDs most of the time is related to the surface groups which corresponds to particle sizes . Normally CDs show strong photoluminescence from blue to green wavelength. To enhance the quantum yield (QY) of CDs or change photoluminescence (PL) emission to meet desired applications, surface passivation and functionality play a vital role. Besides, CDs show great photostability as there are no reductions in PL intensity with continuous exposure to excitation. In terms of chemical properties, different synthesis methods of CDs lead to different chemical structure and abundance of surface sites. They are usually connected or modified by polymer chains, oxygen-based, amino based groups, and others .
Characterization of CDs by high resolution transmission electron microscopy (HRTEM), Xray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR), Atomic force microscopy (AFM) and Zeta Potential provide deep insights into the attributes of CDs such as hybridization and coefficient between functional groups and carbon core that take parts in the provision of the abundance of surface sites and the photoluminescence behaviour . In comparison to graphene and metallic quantum dots, the CDs serves as the way out of toxicity concerns in environmental monitoring and medical applications .
2.2.1 High-resolution transmission electron microscopy (HRTEM)
The sizes and texture of CDs are important for fundamental applications in the field of environmental science and nanotechnology. Figure 4(A–C), shows the HRTEM images of CDs at different resolutions between 1 nm to 10 nm. Synthesized CDs revealed amorphous quasi-spherical morphology with a lattice spacing of ca 0.24 nm (Figure 4A), CDs characteristics are suitable absorbent of pollutants that are larger than 0.24 nm [2, 71, 89].
High-Resolution Transmission Electron Microscopic images of the CDs characterized in magnifications of 5 nm and 10 nm (Figure 4A and B respectively). Figure 4A is the lattice spacing for carbon dots at 5 nm magnification. While Figure 4B is the size distribution within 10 nm magnification. Figure 4C is the histogram chart, demonstrating the nanoparticle sizes of CDs. The synthesis of nanoparticle with low lattice space is needed for research applications of CDs in environmental chemistry, pollutant entrapment in aqueous media and water purification . The interplanar distance (lattice spacing) of 0.24 nm (Figure 4) is lower than the lattice spacing planes of graphitic materials (0.34 nm), the larger interlayer spacing could be attributed to the abundant oxygen-containing groups. In other words, the oxygen-containing groups could expand the layer spacing. The synthesized CDs is in consonance with recent reports by Arumugam and colleagues, CDs was hydrothermally synthesized from broccoli , ginkgo fruits , and cabbage .
2.2.2 Fourier-transform infrared spectroscopy (FTIR)
Fourier-transform Infrared spectroscopy (FTIR) portrays the functional structure of CDs. It reveals the intrinsic functional groups and other useful compounds present in CDs. Figure 5 provides functional groups that exist before and after the hydrothermal treatment of tapioca as a precursor for CDs.
As shown in Figure 5(A) representing tapioca. Peaks associated with the stretching vibrations of hydroxyl (▬OH) and carboxylic (COO▬) groups are at 3353.45 and 2933.78 cm−1 . Further stretching vibration of C▬H occurred from 1645.24 to 1341.82. The peaks at 1151.38, 1079.20, 1014.41 cm−1 can be due to the C▬O stretching vibrations and out-of-plane bending modes of sp2 and sp3 ▬CH group .
There were substantial changes observed in the spectra of CDs (Figure 5B). The hydroxyl (▬OH) group of 3389.71 cm−1 increased on the carbon structure as a result of hydrolysis. While the carboxylic (COO▬) group 2145.73 cm−1 reduced by thermal destruction of saccharides structure . The peaks at 1695.27 cm−1 and 1644.62 cm−1 showed the increase in the C▬H stretching vibrations of the bending modes of the sp2 and sp3 ▬CH group. The peaks around 1427.63 cm−1 until 1369.43 cm−1 are due to C▬O▬C . The peak at 1237.62 cm−1 corresponds to the C〓C stretching vibration while 1094.19 cm−1 and 996.19 cm−1 represents the C〓O stretching vibration and the last group at 706.78 cm−1 denotes the C〓C bond of the unsaturated glucose structure in the starch. These attributes were responsible for the water-soluble nature of CDs . The FTIR graph shows the formation of unsaturated carbon. Along with oxygen-rich groups such as hydroxyl, carboxyl, and carbonyl situated on the CDs surface, which agree with the hydrothermal synthesized CDs from the organic origin [23, 25, 26, 81, 90].
3. Applications of carbon nanoparticles
There are numerous applications of carbon nanoparticles due to the abundant properties they possess . These applications are being discussed in the subsequent sections of the report.
3.1 Application in bioimaging and biomedicals
Carbon dots have shown great potential to act as a sensor and can be used for environmental monitoring and control of pollutants, more so in the medical field for biosensor applications. It can donate or accept electrons that make it suitable for detection of ions, vitamins, nucleic acid, protein, enzyme and biological pH value [7, 11, 96, 97, 98]. Even though different materials are used to detect specific ions, the detection mechanisms are identical .
The functional groups on the surface of CDs specify distinctive affinities to different target ions, through an electron or energy transfer process and high selectivity to other ions . CDs has been involved in the detection of 2,4,6-tri-trotoluene (TNT) and also applied as a dual-sensing platform for fluorescent and electrochemical detection of TNT . Other reports utilized CDs as pH sensors for in-vitro and in-vivo investigations .
Research showed CDs able to detect intracellular pH inside a living pathogenic fungal cell and has been developed to sense nucleic acid in the DNA . In other cases, CDs have been used in bioimaging because of their low toxicity and excellent photostability compared to semi-conductor quantum dots that posed health problems and environmental concerns . Its visible excitation, emission wavelengths, and high brightness confirm CDs as a suitable candidate in this area. Several studies have been conducted using CDs in cell imaging, including pig kidney cell line ,
Chengkun et al.,  discovered photoluminescence in CDs synthesized from Nescafe original instant coffee and applied it in the field of bioimaging. From their investigation, CDs from Nescafe are found to be amorphous and the cytotoxicity study revealed that the CDs did not cause any toxicity to human hepatocellular carcinoma cells at a concentration as high as 20 mg/ml. Yang et al.,  also worked on novel green synthesis of high-fluorescent CDs from honey for sensing and imaging. It was an innovative and green approach towards a CDs of high fluorescent quantum yield and excellent photostability, employed for HeLa cells imaging and coding. Rui-jun et al
3.2 Application in heavy metal ions detection
The application of CDs in the selective detection of heavy metals have been reported in several scientific and experimental research [34, 40, 79]. However, there are gaps and lapses needing redress, such applications are predominantly in photoluminescent quenching of heavy metals. whereas, current section looks into reliable and robust CDs for applications in electrochemical sensing of multiple ranges of heavy metal ions.
The development of a convenient and sustainable technique for detecting and identifying human and environmentally toxic metal ions is of great interest. The following are reports concerning CDs application in heavy metal detection.
Zhang and Chen  worked on nitrogen-doped carbon quantum dots application as a turn-off fluorescent probe for the detection of Hg2+ ions at a detection limit of 0.23 μM. The fluorescent quenching mechanism is attributed to the surface-state triggered by the mercury-induced conversion of special functional group (▬CONH▬) from spirolactam structure to an opened-ring amide .
Sandhya et al.,  applied nanostructures for heavy metal ion sensing in water using surface plasmon resonance of metallic nanostructures. They reviewed on techniques to improve selectivity and sensitivity of surface plasmon response sensors with attention to homogeneity. Effects of particle size, shape, material type, and surrounding environment were found to be effectual in the surface plasmon surface frequency.
Similarly, Qu et al.,  developed CDs to detect Fe3+ ions by using dopamine as a starting material with a detection limit of 0.32 μM. Quenching of photoluminescence intensity occurred when there was an interaction between CDs and ions. Meanwhile, Liu  reported a research work on one-step green synthesized fluorescent carbon nanodots from bamboo leaves for copper (ll) ion detection and demonstrated the exploration of bamboo leaves as a carbon source with high carbon constituent. Carbon quantum dots were synthesized hydrothermally with sensitive Cu2+ detection at limit of detection as low as 115 nM and a dynamic range from 0.333 to 66.6 μM. The zeta potential of the pristine carbon quantum dots was measured at −4.78 mV which improved to +13.8 mV after treatment with positively charged polyethyleneimine (a water-soluble cationic polymer). More so, Rao et al
Methionine has been used as a material for the synthesis of CDs . These CDs were co-doped with nitrogen and sulfur to enhance surface functionalization for the detection and environmental monitoring of heavy metal pollutants . Similarly, Shen et al
A fluorescent probe for selective detection of metal ions such as mercury (Hg2+, 1.00 × 10−8 − 1.50 × 10−3 M, 1.00 × 10−7 M) with wide linear range and satisfactory detection limits was discovered when citric acid monohydrate was used for the synthesis of fluorescent CDs . More essentially and effective is the burning of ash from waste paper and further utilized as a source of CDs by Lin et al., . They succeeded in synthesizing CDs without any surface modification and subsequently, the fluorescent CDs were quenched by Fe3+.
Simpson et al.,  synthesized carbon nanoparticle from glycerol and phosphoric acid mixed in a Berghof high-pressure reactor at 250°C for 4 hours. Afterward, glassy carbon electrodes were fabricated by drop-casting the carbon nanoparticles, and further applied for heavy metal (Cu2+ and Pb2+) detection by square wave anodic stripping voltammetry . Heavy metals such as Na+, K+, Mg2+, Ca2+, Cr3+, Co2+, Ag+, Hg2+, Cd2+, Pb2+, Ni2+, Cu2+, Zn2+, Al3+, Fe2+, and Fe3+ have been tested on CDs synthesized from carbon source of mangosteen pulp and a ground discovery was made. Among the listed heavy metals, Fe3+ was the favourite in detection with a detection limit of 52 nM. Further application was found for cell imaging, which reveals their diverse potential applications .
Abhishek et al.,  made a paper strip based live cell ultrasensitive lead sensor using CDs synthesized from biological media. They reported a formulation of a sensor through microwave heating of potato-dextrose agar (PDA) for the detection of lead (pb2+) in solution but again involved a long and laborious process.
Pajewska et al.  explored the fluorescence of synthesized CDs from citric acid with glutathione for the sensing of mercury (Hg2+) ion. A high recovery of Hg2+ was achieved at 115.1%. The method of synthesizing CDs with low toxicity is embedded in the green chemistry principles. Thus, it fulfills the criteria of being eco-friendly. Table 2 provides a list of applications of CDs in the detection of heavy metals ions.
As seen in Table 3, the mechanism of action for the application of CDs largely depends on the analyte of concern. In the case of CDs from citric acid monohydrate for application in fluorescence quenching of Hg2+, it relies on Förster resonance energy transfer (FRET) . This is similar to CDs synthesized from biomass , polyacrylamide , lotus root , degreased cotton , gold nanoclusters , and Petroleum coke .
|Source of carbon nanoparticles||Sensing mechanism||Type of metal ions and linear range||Sensing (LOD)||Reference|
0.002 mol L−1
|10.3 and 60.9 nM|||
0.1 to 60.0 μM
1 × 10−5 M
|Biomass from peanut shells||Fluorescence||Cu+2|
|Metal oxides||Electrochemical oxidation.||Cu2+|
0.1 to 1.3 μM
0 to 100 nM
|Penaeus merguiensis enzyme||Isotherm||Cu+2|
Fluorescent carbon nanoparticle sensing is largely dependent on changes or disturbances that are caused by an analyte that interacts with a fluorescent probe. This shift mostly will lead to a measurable change in the emission characteristics of the probe (emission wavelength, intensity, lifetime, or anisotropy), which can be directly linked to analytes (e.g heavy metal) concentration. More so, fluorescence probe strategies are based on quenching (turn-off) or enhancing (turn-on) emission, and surface-enhanced Raman scattering (SERS) techniques [33, 125].
Other notable techniques for the detection and quantification of heavy metals ions include, inductively coupled plasma mass spectrometry (ICP-MS). This instrumentation is efficient among several other methods, but it is expensive. It was developed since the 1980s [128, 129, 130], used mostly by multivariate analysis along with the ICP-MS technique to unravel heavy metal elements present samples. However, inductively coupled plasma atomic emission spectroscopy (ICP-AES) have also been used to identify heavy metal pollutants. But, the method is expensive and requires sophisticated instrumentations and a highly trained technician .
Nowadays, marine pollution is becoming a global phenomenon and seafood safety has played a crucial role in human health . Fatema et al.,  applied atomic absorption spectroscopy (AAS) to measure the absorbed quantity of Pb+2, Cd+2, and Hg+3 in shrimps. Heavy metals have been detected by other means such as energy dispersive x-ray fluorescence (EDXRF), electrothermal atomic absorption method (ETAAS), and flame atomic absorption spectroscopy (FAAS) [133, 134]. But, all of the aforementioned techniques have disadvantages in the detection of heavy metals, such that they are expensive and require strenuous experimental steps . Therefore, environmental researchers have continued to strive to develop a cheap, simple, sensitive, specific, accurate, user-friendly, and eco-friendly means of detection for heavy metal pollutants.
3.3 Application in non-metal detection
CDs are very useful in detecting non-metallic elements. Several types of research have been reported, CDs synthesized from potato are well able to detect phosphate . Zhaoxia et al.,  utilized CDs with turnable emission and controlled size for sensing hypochlorous acid. As a class of carbohydrate that is widely distributed in a living organism, sucrose was chosen as a carbon source with assistance of microwave irradiation. A strongly fluorescent CDs without post-passivation was produced. By increasing the concentration of phosphoric acid as fluorescence enhancer under UV lamp, various fluorescent emissions of CDs of variable sizes were obtained. It was found that green CDs have excellent sensitivity for the detection of hypochlorous acid.
Kuo et al
Huilin et al., , investigated on CDs as a fluorescent probe for off-on detection of sodium dodecyl-benzenesulfonate (SDBS) in aqueous solution. The pristine CDs were synthesized from sodium citrate through a simple, convenient, and one-step hydrothermal method. Fluorescent recovery was achieved with the application of SDBS. Detection of SDBS in real water samples was proportional to the concentration in the range of 0.10 to 7.50 ug/mL. Furthermore, fluorescence sensing probe has been used to detect kaempferol (flavonoid that is present in a variety of plants and plant-derived foods) using fluorescent CDs synthesized from chiefly acetic acid with a detection limit of 38.4 nM in the concentration range of 3.5–49 μM. Finally, organophosphorus as pesticides have been detected through the use of CDs as a detector for pollutants without surface modification .
3.4 Application in adsorption studies
CDs and carbon structured nanoparticles have attracted researchers to explore their effectiveness and optimization ability in the fields of pollution research . Because of their excellent properties; carbon material performs concurrently as adsorbent and a transducing-agent [138, 139, 140, 141].
Due to abundant surface sites provided by CDs, it is a suitable candidate for studies in the detection and adsorption of heavy metals [142, 143]. For instance, Ghiloufi et al.,  used gallium doped zinc oxide (ZnO) nanoparticle in the adsorption of heavy metals (Cd2+ and Cr6+) in aqueous solution. The adsorption of heavy metals was analyzed through the effect of pH and it revealed favourable adsorption at a low pH level, less than pH-3 and temperature of 298 K .
Table 4 provides harmonized presentation of nanomaterials applied for the purpose of absorbing environmental pollutants and contaminants in aqueous systems [145, 146, 147, 148, 149, 150, 151, 152, 153, 154].
|Gold nanoparticles (AuNPs)||4-nitrophenol|||
|Carbon dots (sodium citrate)||Mercury (II) ions.|||
|Fluorescent carbon dots from o- phenylenediamine||Cell imaging and sensitive detection of Fe3+ and H2O2|||
|Silica gel||Aromatic volatile organic compounds (VOCs)|||
|Graphene oxide||Nitrobenzene in sulfide|||
|TiO2, SiO2, and ZnO nanoparticles||Neptunium (V)|||
|Polystyrene latex nanoparticles||Alumina|||
|Graphene oxide||Radionuclide removal|||
|Polyaniline modified graphene oxide||Uranium(VI)|||
|Carbon nanotubes||Mingle-ringed N- and S-heterocyclic aromatics|||
|Graphene oxide||Minerals such as montmorillonite, kaolinite, and goethite, in aqueous phase|||
So far the concept of applying nanoparticles for environmental objectives have been successful. Meanwhile it is recommended that comparisons be made with bulk counterparts of the same substance to measure efficiency. On this note a study on the application of bulk agro material from
A suitable carbon source for CDs synthesis should be soluble in water (green chemistry), accessible worldwide (i.e. geographical abundance) with defined and well-known properties (i.e. functional attributes), should not be in direct competition with essential food production (i.e. sustainable), and it should be cost-effective (i.e. cheaply accessible). While the price of additives or carbon source plays a minor role in fundamental research, it may play a major role when large quantities are considered.
The authors would like to thank Universiti Putra Malaysia (UPM), Malaysia for funding this article.
Conflicts of interest
The authors hereby declare that there is no conflict of interest.
M.Y.P., as the first author; made the study conception and design acquisition of reports and drafting of manuscript. Z.Z.A., contributed in the study conception and design, critical revision of major scientific ideas through clinical experience.
This research was funded by Universiti Putra Malaysia, grant number GP-IPS/2017/9556800.
Xu X, Robert R, Yunlong G, Harry JP, Latha G, Kyle R, Walter A. Electrophoretic Analysis and Purification of Fluorescent Single-Walled Carbon Nanotube Fragments. Journal of American Chemical Society. 126: 2004: 12736-12737
Musa YP, Zurina ZA, Suraya AR, Faizah MY, Noor ASM, Mohammed A. Synthesis and Characterization of Fluorescent Carbon Dots from Tapioca. ChemistrySelect, 4: 2019: 1– 8
Das R, Rajib B, Panchanan, P. Carbon quantum dots from natural resource: A review. Materials Today Chemistry. 8: 2018: 96-109
Shen J, Shaoming S, Xiuying C, Dan W, Yan, C. Highly fluorescent N, S-co-doped carbon dots and their potential applications as antioxidants and sensitive probes for Cr (VI) detection. Sensors and Actuators B. 248: 2017: 92-100
Da Silva-Souza DR., Larissa DC., Joao PM., Fabiano VP. Luminescent carbon dots obtained from cellulose. Materials Chemistry and Physics. 203: 2018: 148-155
Sahu S, Behera B, Maiti TK, Mohapatra S. Simple one-step synthesis of highly luminescent carbon dots from orange juice: application as excellent bio-imaging agents. Chemical Communication. 2012: 48: 8835
Bhattacharyya D, Prashant, KS, Michael LF. Quantum dots and carbon dots based fluorescent sensors for TB biomarkers detection. Vacuum. 2017: 146: 606-613
Alam AM, Park BY, Ghouri ZK, Park M, Kim,HY. Synthesis of carbon quantum dots from cabbage with down- and up-conversion photoluminescence properties: excellent imaging agent for biomedical applications. Green Chemistry. 17: 2015: 3791-3797
Bin W, Feng L, Yuanya W, Yanfen C, Chang ML. Synthesis of catalytically active multielement-doped carbon dots and application for colorimetric detection of glucose. Sensors and Actuators B: Chemical. 255: 2018: 2601-2607
Choi Y, Nichaphat T, Ari C, Seongho J, Insik I. Microwave-assisted synthesis of luminescent and biocompatible lysine-based carbon quantum dots. Journal of Industrial and Engineering Chemistry. 47: 2017: 329-335
Ajay KG, Mona G. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials. 26: 2005: 3995-4021
Derfus AM, Chan WCW, Bhatia SN. Probing the cytotoxicity of semiconductor quantum dots. Nano Letters. 4: 2004: 11-19
Wang F, Chen YH, Liu CY, Ma DG. White light-emitting devices based on carbon dots’ electroluminescence. Chemical Communication. 47: 2011: 3502-3504
Abhishek G, Navneen CV, Syamantak K, Shalini T, Abhishek C, Chayan KN. Paper strip based and live cell ultrasensitive lead sensor using carbon dots synthesized from biological media. Sensors and Actuators B: Chemical, 47. 232: 2016: 107-114
Rasheed T, Muhammad B, Faran N, Hafiz MNI, Chuanlong L, Yongfeng Z. Fluorescent sensor based models for the detection of environmentally-related toxic heavy metals. Science of the Total Environment. 615: 2018: 476-485
Hayat A, Marty JL. Disposable screen printed electrochemical sensors: tools for environmental monitoring. Sensors. 14: 2014: 10432-10453
Monoj KB, Bikash J, Santanu B, Amitava P. Photophysical Properties of Doped Carbon Dots (N, P, and B) and Their Influence on Electron/Hole Transfer in Carbon Dots–Nickel (II) Phthalocyanine Conjugates. Journal of Physics and Chemistry C, 118: 2014: 20034-20041
Song Y, Hao L, Fang L, Huibo W, Mengling Z, Jinjing Y, Jian H. Fluorescent carbon dots with highly negative charge as sensitive probe for real-time monitoring of bacterial viability. Journal of Material Chemistry B. 5: 2017: 6008-6015
Edison TNJI, Raji A, Mathur GS, Jae-Jin S, Yong RL. Microwave assisted green synthesis of fluorescent N-doped carbon dots: Cytotoxicity and bio-imaging applications. Journal of Photochemistry and Photobiology B: Biology. 161: 2016: 154-161
Phadke C, Mewada A., Dharmatti R., Thakur M, Pandey S, Sharon M. Biogenic synthesis of fluorescent carbon dots at ambient temperature using azadirachta indica (Neem Gum). Journal of Fluorescent. 8: 2015: 1103-1107
Simpson A, Pandey RR, Charles CC, Kartik G, Adam KW. Fabrication characterization and potential applications of carbon nanoparticles in the detection of heavy metal ions in aqueous media. Carbon. 127: 2018: 122-130
Mewada A, Pandey S, Shinde M, Mishra N, Oza G, Thakur M, Sharon M. Green synthesis of biocompatible carbon dots using aqueous extract of Thrapabispinosa peel. Material Science and Engineering, 56: 2013: 2914-2917
Gedda G, Chun-Yi L, Yu-Chih L, Hui-fen W. Green synthesis of carbon dots from prawn shells for highly selective and sensitive detection of copper ions. Sensors and Actuators B: Chemical. 224: 2016: 396-403
Mehta,VN, Jha S, Singhal RK, Kailasa SK. Preparation of multicolor emitting carbon dots for HeLa cell imaging. New Journal of Chemistry, 38: 2014: 6152-6160
Mehta VN, Jha S, Basu H, Singhal RK, Kailasa SK. One step hydrothermal approach to fabricate carbon dots from apple juice for imaging mycobacterium and fungal cells. Sensors and Actuators B. 213: 2015: 434-443
Kumar D, Singh K., Verma V, Bhatti HS. Synthesis and characterization of carbon quantum dots from orange juice. Bionanoscience. 4: 2014: 274-279
Fernandez M, Dyna M, Suneesh KS, Sobhi D. Green synthesis of nitrogen and sulphur doped carbon dot composites for the sensing of glucose. Materials Today: Proceedings. 9: 2019: 54-60
Wang Y, Hu A. Carbon quantum dots: synthesis, properties and applications. Journal of Materials Chemistry C. 34: 2014: 6921-6939
Mahardika PA, Pradita AW, Jotti K, Annisa LW. Removal of Heavy Metal Nickel-Ions from Wastewaters Using Carbon Nanodots from Frying Oil. Procedia Engineering. 170: 2017: 36 – 40
Wang J, Wang CF, Chen S. Amphiphilic Egg-Derived Carbon Dots: Rapid Plasma Fabrication, Pyrolysis Process, and Multicolor Printing Patterns. Angewandte Chemie International Edition. 51: 2012: 9297-9301
Ke Y, Garg B, Ling YC. Waste chicken eggshell as low-cost precursor for efficient synthesis of nitrogen-doped fluorescent carbon nanodots and their multi-functional applications. Royal Socity of Chemistry Advances. 4: 2014: 58329-58336
Titirici MM, Robin JW, Nicolas B, Vitaliy LB, Dang SS, Francisco DM, James HC, Mark JM. Sustainable carbon materials. Chemical Society Review. 44: 2015: 250-290
Wanekaya AK. Applications of nanoscale carbon-based materials in heavy metal sensing and detection. Analyst. 136: 2011: 4383-4391
Lim SY, Shen W, Gao Z. Carbon quantum dots and their applications. Chemical Society Review. 44: 2015: 362-371
Prat D, Hayler J, Wells A. A survey of solvent selection guides. Green Chemistry. 16: 2014: 4546-4551
Anastas PT, Warner JC. Green chemistry: Theory and practice, Oxford (England) and New York: Oxford University Press. 1998
Wang Y, Hu R, Lin G, Roy I, Yong KT. Functionalized quantum dots for biosensing and bioimaging and concerns on toxicity. American Chemical Society Applied Matererial Interfaces 5: 2013: 2786-2799
Jiang L, Nelson GW, Kim H, Sim I, Han SO, Foord JS. Cellulose-Derived Supercapacitors from the Carbonisation of Filter Paper. Chemistry Open. 4: 2015: 586-589
Derfus AM, Chan AC Warren CW, Bhatia SN. Probing the Cytotoxicity of Semiconductor Quantum Dots. Nano Letter 4: 2004: 11-18
Yuqian P, Hui G, Shaohui W, Xiaolong L. Facile synthesis the nitrogen and sulfur co-doped carbon dots for selective fluorescence detection of heavy metal ions. Materials Letters. 193: 2017: 236-239
Sayan G, Poushali D, Madhuparna B, Subhadip M, Amit, KD, Das NC. Strongly blue-luminescent N-doped carbogenic dots as a tracer metalsensing probe in aqueous medium and its potential activity towardsin situ Ag-nanoparticle synthesis. Sensors and Actuators B. 252: 2017: 735-746
Huang CC, Yi-Shan H, Yih-Ming W, Wenlung C, Yen-Shi L. Sustainable development of carbon nanodots technology: Natural products as a carbon source and applications to food safety. Trends in Food Science & Technology. 86: 2019: 144-152
Pattanayak M, Nayak PL. Ecofriendly green synthesis of iron nanoparticle from various plants. International Journal of Plant. Animal and Environmental Sciences. 78: 2013: 68-78
Liu Y, Xiao N, Gong N, Wang H, Shi X, Gu W, Ye L. One-step microwave-assisted polyol synthesis of green luminescent carbon dots as optical nanoprobes. Carbon. 68: 2014: 258-264
Piyushi N, Subramanian KA, Dastidar MG. Adsorptive removal of dye using biochar derived from residual algae after in-situ transesterification: Alternate use of waste of biodiesel industry. Journal of Environmental Management. 32: 2016: 187-197
Iravani S. Green synthesis of metal nanoparticles using plants. Green Chemistry. 34: 2011: 2638-2650
Wembo L, Xiaoyun Q, Sen L, Guohui C, Yingwei Z, Yonglan L, Abdullah MA, Abdulrahaman OA, Xuping S. Economical, Green synthesis of fluorescent carbon nanoparticles and their use as probes for sensitive and selective detection of mercury (ll) ions. Analytical chemistry. 78: 2012: 5351-5357
Till TM, Piotr JC, Ilko B. White carbon: Fluorescent carbon nanoparticles with tunable quantum yield in a reproducible green synthesis. Scientific Reports. 6: 2016: 28557-28569
Han S, He, Z, Jian Z, Yujie X, Liangliang L, Hangxing W, Xiangkai L, Weisheng L, Yu T. Fabrication, gradient extraction and surface polarity-dependent photoluminescence of cow milk-derived carbon dots. Royal Society of Chemistry Advances. 63: 2014: 58084-58089
Biswajit G, Soubantika P, Joydeep C. Carbon Dots: A Mystic Star in the World of Nanoscience. Journal of Nanomaterials. 10: 2019: 1-19
Gonçalves H, Pedro AS, Jorge JRA, Joaquim CGES. Hg(II) sensing based on functionalized carbon dots obtained by direct laser ablation. Sensors and Actuators B. 145: 2010: 70-82
Thongpoola V, Asanithia P, Limsuwana P. Synthesis of Carbon Particles using Laser Ablation in Ethanol. Procedia Engineering. 32: 2012: 1054 – 1060
Yu H, Li X, Zeng X, Lu Y. Preparation of carbon dots by non-focusing pulsed laser irradiation in toluene. Chemical Communications. 52: 2016: 819-822
David A, Mouhamed D, Julien L, Gilles L, Christophe D. Origin of the nano-carbon allotropes in pulsed laser ablation in liquids synthesis. Journal of Colloid and Interface Science. 489: 2017: 114-125
Myungjoon K, Saho O, Taesung K, Hidenori H, Takafumi S. Synthesis of Nanoparticles by Laser Ablation: A Review. KONA Powder and Particle Journal. 34: 2017: 80-90
Mendonça LT, Azevedo WM. A fast bottom-up route for preparing CdS quantum dots using laser ablation in a liquid environment. Journal of Luminescence.23: 2016: 79-84
Li H, Xiaodie H, Zhenhui K, Hui H, Yang L, Jinglin L, Suoyuan L. Water-soluble fluorescent carbon quantum dots and photocatalyst design. Angewandt Chemie International Edition, 49: 2010: 4430-4434
Shinde DB, Pillai VK. Electrochemical resolution of multiple redox events for graphene quantum dots. Angewandte Chemie International Edition. 52: 2013: 2482-2485
Deng J, Lu Q, Mi N, Li H, Liu M, Xu M, Tan L, Xie Q, Zhang Y, Yao S. Electrochemical synthesis of carbon nanodots directly from alcohols. Chemistry European Journal. 20: 2014: 4993-4999
Yao S, Hu Y, Li G. A one-step sonoelectrochemical preparation method of pure blue fluorescent carbon nanoparticles under a high intensity electric field. Carbon. 65: 2014: 77-83
Zhang P, Xue Z, Luo D, Yu W, Guo Z, Wang T. Dual-peak electrogenerated chemiluminescence of carbon dots for iron ions detection. Analytical Chemistry. 86: 2014: 5620-5623
Yang Y, Zhao B, Gao Y, Liu H, Tian Y, Qin D, Wu H, Huang W, Hou L. Novel hybrid ligands for passivating PbS colloidal quantum dots to enhance the performance of solar cells. Nano-Micro Letters. 7: 2015: 325-331
Tian L, Ghosh D, Chen W. Nanosized Carbon Particles From Natural Gas Soot. Chemistry of Materials, 21: 2009: 2803-2809
Liu H, Ye T, Mao C. Fluorescent Carbon Nanoparticles Derived from Candle Soot. Angewandte Chemie International edition. 63: 2007: 6473-6475
Zhai X, Zhang P, Liu C, Bai T, Li W, Dai L, Liu W. Highly luminescent carbon nanodots by microwave-assisted pyrolysis. Chemical Communications. 48: 2012: 7955-7957
Xinyue Z, Mingyue J, Na N, Zhijun C, Shujun L, Shouxin L, Jian L. Natural-Product-Derived Carbon Dots: From Natural Products to Functional Materials. ChemSusChem. 11: 2018: 11-24
Sun D, Ban R, Zhang P, Wu G, Zhang J, Zhu J. Hair fiber as a precursor for synthesizing of sulfur- and nitrogen-co-doped carbon dots with tunable luminescence properties. Carbon. 56: 2013: 424-434
Zhou P, Lu X, Sun Z, Guo Y, He H. A review on syntheses, properties, characterization and bioanalytical applications of fluorescent carbon dots. Microchimica Acta. 54: 2016: 519-542
Fang Y, Guo S, LI D, ZHu C, Ren W, Dong S, Wang E. Easy synthesis and imaging applications of cross-linked green fluorescent hollow carbon nanoparticles. American Chemical Society Nano: 6: 2012: 400-409
Tang L, Ji R, Cao X, Lin J, Jiang H, Li X, Teng KS, Luk CM, Zeng S, Hao J, Lau SP. Deep ultraviolet photoluminescence of water-soluble self-passivated graphene quantum dots. American Chemical Society Nano. 6: 2012: 5102-5110
Ali AE, Hghighat S, Kazemifard N, Rezaei B, Moradi F. A novel one-step and green synthesis of highly fluorescent carbon dots from saffron for cell imaging and sensing of prilocaine. Sensors and Actuators B: Chemical. 253: 2017: 451-460
Luyao L, Xingxian W, Zheng F, Fengling C. One-step hydrothermal synthesis of nitrogen- and sulfur-co-doped carbon dots from ginkgo leaves and application in biology. Materials Letters. 196: 2017: 300-303
Yongli L, Qingxiang Z, Yongyong Y, Yalin W. Hydrothermal synthesis of fluorescent carbon dots from sodium citrate and polyacrylamide and their highly selective detection of lead and pyrophosphate. Carbon. 115: 2017: 550-560
Jie W, Yuhao C, Fengxian Q, Xin L, Dongya Y. One-pot simple green synthesis of water-soluble cleaner fluorescent carbon dots from cellulose and its sensitive detection of iron ion. Journal of Cleaner Production. 10: 2017: 23-30
Sharma S, Ahmad U, Swati S, Surinder KM, Sushil KK. Photoluminescent C-dots: An overview on the recent development in the synthesis, physiochemical properties and potential applications. Journal of Alloys and Compounds. 748: 2018: 818-853
Yang Y, Cui J, Zheng C. One step synthesized of amino-functionalised fluorescent carbon naoparticles by hydrothermal carbonization of chitosan. Chemical Communications. 39: 2012: 380-382
Zhang B, Liu CY, Liu Y. A Novel One-Step Approach to Synthesize Fluorescent Carbon Nanoparticles. European Journal of Inorganic Chemistry. 28: 2010: 4411-4414
De B, Karak N. A green and facile approach for the synthesis of water soluble fluorescent carbon dots from banana juice. Royal Society of Chemistry Advances. 36: 2013: 1-9
Arumugam N, Jongsung K. Synthesis of carbon quantum dots from Broccoli and their ability to detect silver ions. Materials Letters. 219: 2018: 37-40
Chunxi Z, Yang J, Feng H, Yaling Y. Green synthesis of carbon dots from pork and application as nanosensors for uric acid detection. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 190: 2018: 360-367
Du F, Zhang M, Li X, Li J, Jiang X, Li Z, Gong A. Economical and green synthesis of bagasse derived fluorescent carbon dots for biomedical applications. Nanotechnology. 53: 2014: 315702-315718
Li HT, He XD, Liu Y, Huang H, Lian SY, Lee ST, Kang ZH. One step ultrasonic treatment synthesis of water soluble carbon nanoparticles with excellent photoluminescent properties. Carbon. 49: 2011: 605-609
Liu X, Chunlan Y, Baozhan Z, Jianyuan D, Dan X. Green anhydrous synthesis of hydrophilic carbon dots on large-scale and their application for broad fluorescent pH sensing. Sensors and Actuators B: Chemical. 255: 2018: 572-579
Himaja A, Karthik P, Sreedhar B, Singh SP. Synthesis of Carbon dots from kitchen waste: Conversion of waste to value added product. Fluorescence. 24: 2014: 1767-1773
Thambiraj S, Shankaran DR. Green synthesis of highly fluorescent carbon quantum dots from sugarcane bagasse pulp. Applied Surface Science. 390: 2016: 435-443
Gaddam RR, Sudip M, Neelambaram P, Vasudevan D, Raju VSNK. Facile synthesis of carbon dot and residual carbon nanobeads: Implications for ion sensing, medicinal and biological applications. Materials Science and Engineering: C. 73: 2017: 643-652
Lingling L, Luyao L, Chang-Po C, Fengling C. Green synthesis of nitrogen-doped carbon dots from ginkgo fruits and the application in cell imaging. Inorganic Chemistry Communications. 86: 2017: 227-231
Yaling W, Jingxia Z, Junli W, Yongzhen Y, Xuguang L. Rapid microwave-assisted synthesis of highly luminescent nitrogen-doped carbon dots for white light-emitting diodes. Optical Materials. 73: 2017: 319-329
Yang R, Xiangfeng G, Lihua J, Yu Z, Fedor L. Green preparation of carbon dots with mangosteen pulp for the selective detection of Fe3+ ions and cell imaging. Applied Surface Science. 423: 2017: 426-432
Kasibabu BSB, D'souza SL, Jha S, Singhal RK, Basu H, Kailasa SK. One-step synthesis of fluorescent carbon dots for imaging bacterial and fungal cells. Analytical Methods. 7: 2015: 2373-2382
Xu Y, Wu M, Liu Y, Feng XZ, Yin XB, He XW, Zhang YK. Nitrogen-doped carbon dots: a facile and general preparation method, photoluminescence investigation, and imaging applications. Chemistry: A European Journal. 19: 2013: 2276-2283
Cao L, Sheng-Tao Y, Xin W, Pengju GL, Jia-Hui L, Sushant S, Yamin L, Ya-Ping S. Competitive Performance of Carbon “Quantum” Dots in Optical Bioimaging. Theranostics. 2: 2012: 295-301
Vinci JC, Ferrer IM, Seedhouse SJ, Bourdon AK, Reynard JM, Foster BA, Colon LA. Hidden properties of carbon dots revealed after HPLC fractionation. Physical Chemistry Letters. 65: 2012: 239-253
Zhu GM, Zeng Y, Zhang L, Tang J, Chen M, Cheng LH, Zhang L, He Y, Guo XX, He MY. Highly sensitive electrochemical sensor using a MWCNTs/GNPs-modified electrode for lead (II) detection based on lead(II)-induced G-rich DNA conformation. Analyst. 139: 2014: 5014-5020
Gho EJ, Ki SK, Yi RK, Ho SJ, Songeun B, Won HK, Giuliano S, Seok HY, Sei KH. Bioimaging of Hyaluronic Acid Derivatives Using Nanosized Carbon Dots. Biomacromolecules. 13: 2012: 2554-2561
Vadivel R, Senthil KT, Kaviyarasan R, Ragupathy S, Rajkumar S, Perumal R. Outright Green Synthesis of Fluorescent Carbon Dots from Eutrophic Algal Blooms for In Vitro Imaging. American Chemical Society Sustainable Chemical Engineering. 9: 2016: 4724-4731
Cheng IW, Huan TC, Chia HL, Yu WS, Binesh U, Yu J, Chih CH. One-step synthesis of biofunctional carbon quantum dots for bacterial labeling. Biosensors and Bioelectronics. 68: 2015: 1-6
Chengkun J, Hoa W, xiaojie S, xiaojun M, Jihui W, Mingquian T. Presence of photoluminescent carbon dots in Nescafe original instant coffee: Application to bioimaging. Talanta. 10: 2014: 68-74
Kuo-Chih L, Han-sheng C, Shu-yu F, You-Di T, Pei-Hsuan L. Percutaneous fiber-optic biosensors for immediate evaluation of chemotherapy efficacy in vivo (part 1): Strategy of assay design for monitoring non-homogeneously distributed biomarkers. Sensors and Actuators B: Chemical. 45: 2016: 544-550
Musa YP, Zurina ZA, Suraya AR, Faizah MY, Noor ASM, Jaafar A. Selective and simultaneous detection of cadmium, lead and copper by tapioca-derived carbon dot–modified electrode. Environmental Science and Pollution Research. 27: 2020: 13315-13324
Lingling Z, Yujie H, Zhu Y, Shaojun D. Simple and Sensitive Fluorescent and Electrochemical Trinitrotoluene Sensors Based on Aqueous Carbon Dots. Analalytical Chemistry. 874: 2015: 2033-2036
Miaoran Z, Rigu S, Jian Z, Ling F, Wei C, Qingwen G, Weijun L, Neng L, Yusheng C, Lulu, C. Red/orange dual-emissive carbon dots for pH sensing and cell imaging. Nano Research. 12: 2019: 815-832
Li Y, Hu Y, Zhao Y, Shi G, Deng L, Hou Y, Qu L. An electrochemical avenue to green-luminescent graphene quantum dots as potential electron-acceptors for photovoltaics. Advance Materials. 23: 2011: 776-780
Hsu PC, Shih ZY, Lee CH, Chang HT. Synthesis and analytical applications of photoluminescent carbon nanodots. Green Chemistry. 14: 2012: 917-921
Sun Y, Zhou B, Lin Y. Quantum-sized carbon dots for bright and colorful photoluminescence. Journal of American Chemical Society. 53:: 2006: 7756-7757
Xu J, Zhou Y, Cheng G, Dong M, Liu S, Huang C. Carbon dots as a luminescence sensor for ultrasensitive detection of phosphate and their bioimaging properties. Luminescence. 42: 2015: 411-415
Yang X, Zhuo Y, Zhu S, Luo Y, Feng Y, Dou Y. Novel and green synthesis of high-fluorescent carbon dots originated from honey for sensing and imaging. Biosensors Bioelectronic. 60: 2014: 292-298
Rui-Jun F, Qiang S, Ling Z, Yan Z, An-Hui L. Photoluminescent carbon dots directly derived from polyethylene glycol and their application for cellular imaging. Carbon. 71: 2014: 87-93
Zhang R, Chen W. Nitrogen-doped carbon quantum dots: facile synthesis and application as a “turn-off” fluorescent probe for detection of Hg2+ ions. Biosensors and Bioelectronic. 55: 2014: 83-90
Sandhya BDB, Tanujjal B, Sunandan B, Joydeep D. Heavy Metal ion Sensing in water using surface plasmon resonance of metallic nanostructures. Groundwater for sustainable development. 1:2015: 1-11
Qu K, Wang J, Ren J, Qu X. Carbon dots prepared by hydrothermal treatment of dopamine as an effective fluorescent sensing platform for the label-free detection of iron(III) ions and dopamine. Chemistry – A European Journal. 22: 2013: 7243-7252
Rao L, Yong T, Zongtao L, Xinrui D, Binhai Y. Efficient synthesis of highly fluorescent carbon dots by microreactor method and their application in Fe3+ ion detection. Materials Science and Engineering: C. 81: 2017: 213-223
Pang Y, Hui G, Shaohui W, Xiaolong L. Facile synthesis the nitrogen and sulfur co-doped carbon dots for selective fluorescence detection of heavy metal ions. Materials Letters. 193: 2017: 226-239
Han M, Liping W, Siheng L, Liang B, Zhenhui K. High-bright fluorescent carbon dot as versatile sensing platform. Talanta. 174: 2017: 265-273
Lin B, Yun Y, Manli G, Yujuan C, Duo W. Modification-free carbon dots as turn-on fluorescence probe for detection of organophosphorus pesticides. Food Chemistry. 77: 2017: 6-11
Pajewska-Szmyt M, Bogusław B, Renata GK. Sulphur and nitrogen doped carbon dots synthesis by microwave assisted method as quantitative analytical nano-tool for mercury ion sensing. Materials Chemistry and Physics: 242: 2020: 1-9
Ye Q, Fanyong Y, Yunmei L, Yinyin W, Li C. Formation of N, S-codoped fluorescent carbon dots from biomass and their application for the selective detection of mercury and iron ion. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 173: 2017: 854-862
Hua J, Jian Y, Yan Z, Chunxi Z, Yaling Y. Highly fluorescent carbon quantum dots as nanoprobes for sensitive and selective determination of mercury (II) in surface waters. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 187: 2017: 149-155
Gu D, Shaoming S, Qin Y, Jie S. Green synthesis of nitrogen-doped carbon dots from lotus root for Hg(II) ions detection and cell imaging. Applied Surface Science. 390: 2016: 38-42
Wang J, Fengxian Q, Xin L, Haiyan W, Dongya Y. A facile one-pot synthesis of fluorescent carbon dots from degrease cotton for the selective determination of chromium ions in water and soil samples. Journal of Luminescence 188: 2017: 230-237
Fang Y, Song J, Li J, Wang Y, Yang H, Sun J, Chen G. Electrogenerated chemiluminescence from Au nanoclusters. Chemical Communications. 47: 2011: 2369-2371
Ma X, Yuanhua D, Hanyuan S, Ningsheng C. Highly fluorescent carbon dots from peanut shells as potential probes for copper ion: The optimization and analysis of the synthetic process. Materials Today Chemistry. 5: 2017: 1-10
Liu W. Preparation of a Zinc Oxide-Reduced Graphene Oxide Nanocomposite for the Determination of Cadmium(II), Lead(II), Copper(II), and Mercury(II) in Water. International Journal of Electrochemical Science. 12: 2017: 5392 – 5403
Rahmanian O, Mohammad D, Mahmood KA. Carbon quantum dots/layered double hydroxide hybrid for fast and efficient decontamination of Cd(II): The adsorption kinetics and isotherms. Applied Surface Science. 428: 2018: 272-279
Venkateswarlu S, Buddolla V, Ankireddy SR, Minyoung Y. Fungus-derived photoluminescent carbon nanodots for ultrasensitive detection of Hg2+ ions and photoinduced bactericidal activity. Sensors and Actuators B: Chemical. 258: 2018: 172-183
Homaei A. Immobilization of Penaeus merguiensis alkaline phosphatase on gold nanorods for heavy metal detection. Ecotoxicology and Environmental Safety. 136: 2017: 1-7
Wang Y, Wen-ting W, Ming-bo W, Hong-di S, Jie-shan Q. Yellow-visual fluorescent carbon quantum dots from petroleum coke for the efficient detection of Cu2+ ions. New Carbon Materials. 30: 2015: 550-559
Simona T, Michaela VG, Kristýna Š, Zuzana Č, Viktor K. Study of metal accumulation in tapeworm section using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Microchemical Journal. 133: 2017: 380-390
Beauchemin D, Inductively Coupled Plasma Mass Spectrometry Methods. Encyclopedia of Spectroscopy and Spectrometry. 3: 2017: 236-245
Tokalıoğlu S. Determination of trace elements in commonly consumed medicinal herbs by ICP-MS and multivariate analysis. Food Chemistry. 134: 2012: 2504-1512
Isai KA, Shrivastava VS. Detection and Identification of Organics and Metals from Industrial Wastewater by ICP-AES. Journal of Advanced Chemical Sciences. 1: 2015: 164-166
Fatema K, Naher K, Choudhury TR, Islam MA, Tamim U. Determination of Toxic Metal Accumulation in Shrimps by Atomic Absorption Spectrometry (AAS). Journal of Environmental Analytical Chemistry. 2: 2015: 140-153
Teerawat P, Natnicha S, Atitaya S. Use of electrothermal atomic absorption spectrometry for size profiling of gold and silver nanoparticles. Analytica Chimica Acta. 1000: 2018: 75-84
Hisham KF, Randa ME, Mohamed DH. The Application of Flame Atomic Absorption Spectrometry for Gold Determination in Some of Its Bearing Rocks. American Journal of Analytical Chemistry. 6: 2015: 411-421
Steve JH, Andy SF. Atomic Absorption, Methods and Instrumentation. Encyclopedia of Spectroscopy and Spectrometry (Third Edition). 2017: 37-43
Zhaoxia H, Feng L, Ming H, Chunxiang L, Ting X, Chuan C, Xiangqun G. Carbon dots with turnable emission, Controlled size and their application for sensing hypochlorous acid. Journal of Luminescence. 51: 2014: 100-105
Huilin T, Xiufen L, Qingyi W, Xiangli X, Fuxin Z, Zhongsheng Y, Mei Q, Zhenli W. Carbon dots as fluorescent probe for “off-on” Detecting sodium dodecyl-benzenesulfonoate in aqueous solution. Spectrochimica Acta part A: Molecular and Biomolecular Spectroscopy. 153: 2016: 268-272
Pumera M. The electrochemistry of carbon nanotubes: fundamentals and applications. Chemistry- A European Journal. 20: 2009: 4970-4978
Musa YP, Zurina ZA, Suraya AR, Faizah MY, Noor ASM, Mohammed A. Eco-Friendly Sustainable Fluorescent Carbon Dots for the Adsorption of Heavy Metal Ions in Aqueous Environment. Nanomaterials. 10: 2020: 315-331
Trojanowicz M. Analytical applications of carbon nanotubes: a review. Trends in Analytical Chemistry. 25: 2006: 480-489
Musameh MM, Hickey M, Kyratzis IL. Carbon nanotube-based extraction and electrochemical detection of heavy metals. Research on Chemical Intermediates. 37: 2011: 675-687
Burakova AE, Evgeny VG, Irina VB, Anastassia EK, Shilpi A, Alexey GT, Vinod KG. Adsorption of heavy metals on conventional and nanostructured materials for wastewater treatment purposes: A review. Ecotoxicology and Environmental Safety. 148: 2018: 702-712
Musa YP, Zainal ZA, Abdul RS, Md Yasin F, Noor ASM, Issa MA. Sustainable Synthesis Processes for Carbon Dots through Response Surface Methodology and Artificial Neural Network. Processes. 10: 2019: 704-723
Ghiloufi I, ElGhoul J, Modwi L. Ga-doped ZnO for adsorption of heavy metals from aqueous solution. Materials Science in Semiconductor Processing. 42: 2016: 102-106
Ruihua L, Juan L, Weiqian K, Hui H, Xiao H, Xing Z, Yang L, Zhenhui K. Adsorption dominant catalytic activity of a carbon dots stabilized gold nanoparticles system. Dalton Transactions. 43: 2014: 10920-10929
Guo Y, Wang Z, Shao H, Jiang X. Hydrothermal synthesis of highly fluorescent carbon nanoparticles from sodium citrate and their use for the detection of mercury ions. Carbon. 52: 2014: 583-589
Susmita D, Susanta KB, Raz J. Carbon-dot-aerogel sensor for aromatic volatile organic compounds. Sensors and Actuators B. 241: 2017: 607-613
Fu H, Zhu D. Graphene oxide-facilitated reduction of nitrobenzene in sulfide-containing aqueous solutions. Environmental Science and Technology. 47: 2013: 4204-4210
Müller K, Foerstendorf H, Brendler V, Bernhard G, Sorption of Np(V) onto TiO2, SiO2, and ZnO: An in situ ATR FT-IR spectroscopic study. Environmental Science and Technology. 43: 2009: 7665-7670
Quevedo IR, Olsson AL, Tufenkji N. Deposition kinetics of quantum dots and polystyrene latex nanoparticles onto alumina: role of water chemistry and particle coating. Environmental Science and Technology. 47: 2013: 2212-2220
Romanchuk AY, Slesarev AS, Kalmykov SN, Kosynkin DV, Tour JM. Graphene oxide for effective radionuclide removal. Physical Chemistry Chemical Physics. 15: 2013: 2321-2327
Shao D, Hou G, Li J, Wen T, Ren X, Wang X. PANI/GO as a super adsorbent for the selective adsorption of uranium (VI). Chemical Engineering Journal. 255: 2014: 604-612
Wang L, Zhu D, Duan L, Chen W. Adsorption of single-ringed N- and S-heterocyclic aromatics on carbon nanotubes. Carbon. 48: 2010: 3906-3915
Zhao J, Liu F, Wang Z, Cao X, Xing B. Heteroaggregation of graphene oxide with minerals in aqueous phase. Environmental Science and Technology. 49: 2015: 2849-2857
Musa YP, Zurina Z A, A sustainable and eco-friendly technique for dye adsorption from aqueous media using waste from Jatropha curcas (isotherm and kinetic model). Desalination and Water Treatment. 182: 2020: 365-374
Park JH, Yong SO, Seong-Heon K, Ju-Sik C, Jong-Soo H, Ronald D, Dong-Cheol S, Competitive adsorption of heavy metals onto sesame straw biochar in aqueous solutions. Chemosphere. 142: 2016: 77-83