Cotton, a major source of natural fiber and vegetable oil , belongs to the genus
Significant progress toward increasing lint yield and improving lint quality has been made during the last seven decades through using conventional and nonconventional breeding approaches. Transgenic cotton conferring resistance to chewing insect pests as well as to glyphosate not only reduces the cost of production but also increases the yield per unit area in developing countries. Cultivation of transgenic cotton helped IPM programs as well as reduced the toxic impact of pesticide in the environment. Also, the cultivation of
2. Possibilities toward achieving sustainable cotton production
Bringing sustainability in cotton production is a major challenge for the resource-poor farming communities—dominantly living in developing countries. Other than releasing cotton varieties expressing high yield potential by growing in high input environments—progressive farmers can afford, there is a need to open R&D fronts for developing cotton varieties which can withstand the impact of changing climate and produce sustainable yields in low input or in optimum input environments . Deployment of high-tech genomic tools in breeding is one of the approaches to initiate breeding by design aiming at the development of resilient cotton cultivars [12, 13].
Efforts for achieving maximum yield potential under optimum input environments are not successful largely because of lack of genetic diversity among the genetic resource use to develop new cotton varieties. Narrow genetic base does not only limit the future breeding progress but also makes the crop vulnerable to insect pests and diseases and also to the negative impact of changing climate. For enhancing the genetic diversity of newly developed cultivars, underutilized genetic resources, land races, obsolete varieties, old accessions, etc. are the golden assets for cotton breeders to cultivate genes conferring novel traits . Usually, cotton breeders avoid using the genetic resource from undomesticated germplasm as it hinders the breeding progress because of the linkage drag of some undesirable traits present in the wild germplasm. For having success using conventional breeding approach, selection of diverse parent genotypes, appropriate population size, and accurate testing of newly developed lines in a given ecosystem are the prerequisites to develop cotton varieties with improved genetics .
After sequencing of
Another important genetic resource in the form of genetic maps construed using several mapping populations developed through interspecific (
In developing countries, cotton breeders are not using the diagnostic markers linked with simple traits because of lack of resources and trained manpower. It is extremely important to identify DNA markers close enough to the complex traits in spite of the fact that genetic base of the parent genotypes is narrow. In this regard, collaborative efforts among few labs for searching new DNA markers have been initiated by deploying new assays like nested-association mapping and association mapping approaches. Such coordinated efforts can be successful if the goal is common, for example, breeding for resistance to lepidopteron insect pests, drought, and salinity. However, there are few research issues typical to a specific region. For example, cotton leaf curl disease infects cotton in Pakistan and is a potential threat to all other cotton-growing countries where whitefly is prevalent. For addressing this issue, collaborative and coordinated research programs including sharing of cotton germplasm for screening in the hotspot regions would help in tackling such issues. USDA screened >5000 accessions of cotton in hotspot regions of Pakistan, and more than a dozen of asymptomatic cotton genotypes were identified [11, 13, 19]. This information and genetic material are useful for Pakistan and also for the whole cotton-growing community as the threat is spreading to other countries (reported in India and China). Thus, cotton production would be sustained. There are 53,000–63,946 cotton germplasm accessions that are preserved in gene banks of different cotton-growing countries. Sharing these resources is extremely important to characterize the extent of phenotypic and genotypic diversity present in the genus
Another important genetic resource is the cultivated
The use of mutagens for breaking the negatively linked traits has been used to develop new cotton varieties. Earlier radiations (gamma rays) have been extensively used during the early 1960s—induced changes in structure of chromosomes randomly. Usually, soaked seed and/or pollen grains are exposed to physical mutagens. These mutagens can be used to expose F1/F2 seed developed through crossing two different species, and the best mutant plants can be identified by surveying with DNA markers originating from the adapted species. This technique was not largely found worth working because of induction of several deleterious mutations and unwanted linkages in the newly developed mutated genotypes.
The use of chemical mutagens is another approach for inducing mutations by exposing the cottonseed with known mutagens; that is, EMS would not only be helpful in assigning functions to different genes but would also be instrumental in adding new alleles through adding or deleting or replacing nucleotides in the genes which would enhance the genetic diversity—a potential buffer to the epidemic of insect pests and diseases.
Hybrid vigor, increased growth over the parent genotypes, has been explored in corn that resulted in multifold increase in production worldwide. Such efforts were also translated in vegetable and arable crops including cotton but could not gain the popularity like corn. For example, in few parts of the world, cultivation of hybrid cotton has shown increase in lint production. The hybrid cotton can be exploited if the hybrid seed surpasses the yield by 30% over the open-pollinated variety (OPV); resultantly, farmers may get rewards of their investments made to procure costly hybrid seed. In this regard, extensive studies are needed to identify the best combiners. Hybrid cotton breeding is also handicapped due to the nonavailability of reliable genetic as well as mechanical means for getting rid of anthers/pollens. Farmers may produce hybrid seed sufficient to meet their own demands (farmers in few provinces of China do this practice) for sowing cotton hybrids instead of OPVs. Thus, farmers can save money meant for buying the hybrid seed. Trainings for the production of hybrid cottonseed may be given to farmers by the public sector organizations [11, 22].
In most developing countries, for example, in Pakistan, the cost of cottonseed is paid based on total weight of the cottonseed irrespective of the lint potential which encourages farmers to grow varieties producing more cottonseed yield per hectare rather than more lint yield per hectare. A significant fluctuation in lint percentage (35–50%) in the cotton germplasm has been found. Thus, emphasis should be made to breed for >45% lint recovery which would add a couple of million bales toward the total cotton production.
Another breeding objective is to improve the lint quality—always remained a major challenge for breeders as the trait is controlled by multiple thousands of genes. Through conventional breeding, limited success has been achieved; however, further improvement is handicapped due to lack of compatible genetic resources. The success of resolving the complex traits has been demonstrated in model plant species using modern genomic tools. Currently, efforts are underway to clone QTLs involved in conferring these complex traits in cotton. Genome sequence information of the cultivated species also unraveled the genetics of fiber initiation—relatively simpler than that of the fiber elongation traits. Once the key genes involved in defining lint characters are identified and these can be used to engineer the pathways of the diploid cotton species like
Protection to insect pests and diseases has been engineered in different crop species including cotton using different novel genes (Cry genes) excised from a soil bacterium. Success of
Identification of new marker genes (e.g., genes conferring fluorescent proteins) instead of using conventional marker genes (e.g., antibiotic-resistant gene) would be useful for making the use of GM foods more safe. These genes should be tested for an extended time period on the number of different model organisms for making the conclusions acceptable to the public in effective manner. All these new genes from alien background may be supplemented, or resistance can be delayed by adopting some other strategies including development of short-duration varieties and characters offering defense umbrella (small leaves, pubescence, light green leaves, etc.) for avoiding any significant damage by the insect pests and diseases. Secondly, research on other aspects such as chemical ecology would add synergy in managing eco-friendly insect pests and diseases and thus can enhance yield.
Genome editing through CRISPR-Cas is an emerging tool which can be used to edit the genes to improve or silent their expression. For example, gossypol-free cottonseed can be produced by silencing the genes conferring gossypols in seed. Major advantage of this assay is that the function of gene can be characterized and new cultivars can be evolved without introgressing foreign gene; hence, the technology will be acceptable to countries having skeptical views about the GM technology [11, 12, 15, 17]. Thus, it is summarized that the adoption of high-tech management practices, utilization of untapped genetic resources in breeding, cultivation of cotton varieties with excellent genetics, monitoring of risk and efficacy of transgene in ecosystem, and continued search for new genetic resources would help in sustaining cotton production [11, 12].
Cotton breeding, largely based on recombination genetics, has paved the way to develop cotton varieties which were heat tolerant, early maturing, and high yielding with improved fiber traits. Historically, the breeding subject of cotton revolves around the evolution of new cultivars, maintenance of varieties, and their seed production. Presently, phenotypic-based selection procedures have been changed to DNA-based selection systems (marker-assisted selection)—made possible for evolving cotton varieties with brilliant genetics in the shortest possible time. Finding the enormous number of variations has been greatly facilitated by the advent of next-generation sequencing tools, and these variations were assigned functions using advanced bioinformatic tools. All these discoveries and effectiveness of the technologies have made it possible to initiate breeding by design using DNA markers as well as by precise genome editing. Unlike conventional breeding practices, genes (e.g., Cry1Ac, etc.) have been transferred from alien sources for improving resilience to chewing insect pests and herbicides. In the present book, research efforts representing a wide range of research endeavors, being undertaken in different parts of the world, were comprehensively discussed. Both the editors (Drs. Mehboob-ur-Rahman and Yusuf Zafar) ensured the compilation of high-quality research, opinions, and progress made toward enhancing and understanding the cotton genome and its application for developing resilient cotton varieties—a way to sustain cotton production beyond 2050. In the end, editors acknowledge efforts and hard work of the authors in compiling their respective chapters.