Link parameter values.
\r\n\t
",isbn:"978-1-83962-366-0",printIsbn:"978-1-83962-365-3",pdfIsbn:"978-1-83962-367-7",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"f7602ad192ad1dc79a6a37ce3b461769",bookSignature:"Prof. Hechmi Toumi and Dr. Eric Lespessailles",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11720.jpg",keywords:"Xray, MRI, Arthroscopy, Nonsteroidal Anti-inflammatory Drugs, Steroid Injections, Surgery, Muscle, Obesity, Age, Inflammation, Plants, Herbs",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"April 13th 2022",dateEndSecondStepPublish:"May 11th 2022",dateEndThirdStepPublish:"July 10th 2022",dateEndFourthStepPublish:"September 28th 2022",dateEndFifthStepPublish:"November 27th 2022",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"2 months",secondStepPassed:!0,areRegistrationsClosed:!1,currentStepOfPublishingProcess:3,editedByType:null,kuFlag:!1,biosketch:"Professor Hechmi Toumi is a researcher, and scientific director of the Transitional Medical Research PRIMMO, CHR Hospital Orleans. His research focuses on Bone, Cartilage, and soft tissue injuries. He has lectured worldwide on the Treatment and Prevention of muscular-skeletal injuries. He is the inventor of the international patent filing on the use of algorithmic analyses to identify and quantify ‘invisible’ detail in soft tissues in standard radiographs.",coeditorOneBiosketch:"Dr. Eric Lespessailles is a Medical Doctor in Rheumatology at the Regional Hospital of Orleans in France. He was awarded a Professorial title by CNU France (Conseil National Universitaire, section 66) in 2019. Eric is currently the medical director of the Translational Medicine Research Platform at the Regional Hospital of Orleans. He has published about 200 peer-reviewed manuscripts. His research focuses on osteoporosis, osteoarthritis, psoriatic arthritis, and musculoskeletal imaging.",coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"196403",title:"Prof.",name:"Hechmi",middleName:null,surname:"Toumi",slug:"hechmi-toumi",fullName:"Hechmi Toumi",profilePictureURL:"https://mts.intechopen.com/storage/users/196403/images/system/196403.png",biography:"Professor Hechmi Toumi graduated from Blaise Pascal University in France. He is a research assistant at the University of Wisconsin in the USA and at Cardiff University and is an awarded professor at the University of Wales, UK. He has acted as dean of the Faculty of Sciences at the University of Orleans from 2013 to 2020. Currently, Professor Toumi is the scientific director of Translational Medical Research Platform, PRIMMO, at the Orleans Hospital in France and vice president of the Institute of Research, Treatment, and Prevention of Osteoporosis. He is currently filing for an international patent application for his invention on the use of algorithmic analyses to identify and quantify invisible detail in soft tissues using standard radiographs. Professor Toumi has lectured worldwide on the pathologies, treatment, and prevention of muscular-skeletal injuries.",institutionString:"University of Orléans",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"2",institution:{name:"University of Orléans",institutionURL:null,country:{name:"France"}}}],coeditorOne:{id:"379966",title:"Dr.",name:"Eric",middleName:null,surname:"Lespessailles",slug:"eric-lespessailles",fullName:"Eric Lespessailles",profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:"Dr. Eric Lespessailles is a Medical Doctor in Rheumatology at the Regional Hospital of Orleans in France. Eric obtained a Ph.D. in Sciences in 2009. He was awarded a Professorial title by CNU France (Conseil National Universitaire, section 66) in 2019. Eric is currently the medical director of the Translational Medicine Research Platform at the Regional Hospital of Orleans. He has published about 200 peer-reviewed manuscripts. His research focuses on osteoporosis, osteoarthritis, psoriatic arthritis, and musculoskeletal imaging.",institutionString:"University of Orléans",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:null},coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"429341",firstName:"Paula",lastName:"Gavran",middleName:null,title:"Ms.",imageUrl:"//cdnintech.com/web/frontend/www/assets/author.svg",email:"paula@intechopen.com",biography:null}},relatedBooks:[{type:"book",id:"7565",title:"Osteoarthritis Biomarkers and Treatments",subtitle:null,isOpenForSubmission:!1,hash:"e1f50f84936fc4164de08483e43acc16",slug:"osteoarthritis-biomarkers-and-treatments",bookSignature:"Hechmi Toumi and Marija Mazor",coverURL:"https://cdn.intechopen.com/books/images_new/7565.jpg",editedByType:"Edited by",editors:[{id:"196403",title:"Prof.",name:"Hechmi",surname:"Toumi",slug:"hechmi-toumi",fullName:"Hechmi Toumi"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"11031",title:"Rheumatoid Arthritis",subtitle:null,isOpenForSubmission:!1,hash:"b27b90045995c761c0d2f975e895c5d4",slug:"rheumatoid-arthritis",bookSignature:"Hechmi Toumi",coverURL:"https://cdn.intechopen.com/books/images_new/11031.jpg",editedByType:"Edited by",editors:[{id:"196403",title:"Prof.",name:"Hechmi",surname:"Toumi",slug:"hechmi-toumi",fullName:"Hechmi Toumi"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6550",title:"Cohort Studies in Health Sciences",subtitle:null,isOpenForSubmission:!1,hash:"01df5aba4fff1a84b37a2fdafa809660",slug:"cohort-studies-in-health-sciences",bookSignature:"R. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2270",title:"Fourier Transform",subtitle:"Materials Analysis",isOpenForSubmission:!1,hash:"5e094b066da527193e878e160b4772af",slug:"fourier-transform-materials-analysis",bookSignature:"Salih Mohammed Salih",coverURL:"https://cdn.intechopen.com/books/images_new/2270.jpg",editedByType:"Edited by",editors:[{id:"111691",title:"Dr.Ing.",name:"Salih",surname:"Salih",slug:"salih-salih",fullName:"Salih Salih"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"52880",title:"Stabilization Control for the Giant Swing Motion of the Horizontal Bar Gymnastic Robot Using Delayed Feedback Control",doi:"10.5772/65831",slug:"stabilization-control-for-the-giant-swing-motion-of-the-horizontal-bar-gymnastic-robot-using-delayed",body:'In the past decades, scientists have found that unusual and unexpected evolution patterns arise frequently in numerous natural and model nonlinear systems in physics, chemistry, biology, engineering, medicine, economics, and so forth, since Lorenz [1] in 1963, May [2] in 1976, and others reported chaotic behavior in very simple dynamical models. The most peculiar aspect of these patterns is their random-like behavior, although the systems are deterministic, in other words, the deterministic nature of these systems does not make them predictable [3]. This behavior is known as deterministic chaos, or simply chaos. It is considered that chaos is due to sensitive dependence on the initial conditions. This property implies that two trajectories emerging from two different close-by initial conditions separate exponentially in the course of time. Due to this property, and due to the fact that, in general, experimental initial conditions are never known perfectly, chaotic systems cannot be predicted by their long-term behaviors.
This feature of their critical sensitivity to initial conditions, often regarded as a troublesome property, made chaos undesirable in engineering control practice, and most experimentalists considered such characteristics as something to be strongly avoided since it restricts the operating range of many electronic and mechanic devices. Besides that, chaotic systems exhibit two other important properties. One is that there is an infinite number of unstable periodic orbits embedded in the underlying chaotic set. The other is that the dynamics in the chaotic attractor is ergodic; that is to say, the system visits ergodically small neighborhood of every unstable fixed point during its temporal evolution [4].
Although the existence of steady states and an infinity of different unstable periodic orbits embedded in chaotic motion is not usually obvious in free-running chaotic evolution, these orbits offer a great potential advantage if one wants to control a chaotic system. The presence of chaos may be a great advantage for control in a variety of situations. In a nonchaotic system, small controls typically can only change the system dynamics slightly. However, in a chaotic system, one can choose between a rich variety of dynamical behaviors. It is then not surprising that the matter of controlling chaotic systems has come under detailed investigation by several different scientific communities. Chaotic dynamics consists of a motion where the system moves in the neighborhood of one of the unstable periodic orbits (UPO) for a while, then falls close to a different UPO and remains for a limited time. Thus, it allows to exploit a single dynamical system for the production of a large number of different periodic behaviors so that the single system can carry out different performances with different yields [5]. The result is to render an otherwise chaotic motion more stable and predictable, which is often an advantage. The perturbation must be tiny, to avoid significant modification of the system’s natural dynamics.
Since Ott, Grebogi, and Yorke [6], in 1990, pointed out the existence of many unstable periodic orbits (UPOs) embedded in chaotic attractors that raise the possibility of using very small external forces to obtain various types of regular behavior, Pyragas [7] in 1992, proposed a so-called “Delayed Feedback Control (DFC)” idea that an appropriate continuous controlling signal formed from the difference between the current state and the delayed state is injected into the system, whose intensity is practically zero as the system evolves close to the desired periodic orbit but increases when it drifts away from the desired orbit, several techniques were devised for controlling chaos [8–21] during the past years and applied to various systems [22–31]. It is worth noting that in spite of the enormous number of applications among the chaos control, very few rigorous results are so far available. Most results are justified by computer simulations rather than by analytical tools. Therefore, many problems remain unsolved.
On the other hand, recently in the field of mechanical and control engineering, the open-loop link mechanism, in terms of diversity and ease of movement, has been used in industrial robots, although a large control input is needed due to speeding up, and the opposing force to the joint and the base increases. Therefore, the actuator with a large driving performance is needed, and the mass of the entire mechanism is increased. It is thought that the control method using the opposing force and gravity is important to achieve the desired movement with high speed and high efficiency by the limited driving performance. The joint type movement robot is actively researched in recent years, and the achievement of dynamic walking and running with high speed and efficiency becomes a problem. Although living creatures, such as humans or animals, have achieved the high-speed movement by using the open-loop link mechanism, it is thought that this is because that a good movement possessing the energy efficiency and small joint drive power and small impact power, and so forth in the gravitational filed had been acquired adaptively during the process of evolution. For example, the human biped locomotion is performed by dynamic link mechanism coupled to the pendulum motion [32, 33], and brachiation (movement across branches) used by the monkeys is a specialized form of arboreal locomotion that can be seen as a continuous pendulum motion [34, 35]. In addition, humans have competed for the manipulation of their own multi-degree of freedom link mechanism through sports or physical exercise, and so forth, and the achievement of a complex movement meeting a specific initial terminal requirement as a technique.
The systems, such as humans and robots, which control the internal force to achieve motions by using reaction force from the outside world, can be modeled as an underactuated link mechanism. These systems usually have nonholonomic second-order constraints due to the presence of passive joints, which makes development of control methods a difficult task. In recent years, studies on underactuated mechanical systems that possess fewer actuators than degrees of freedom have received increased interest [36–40].
Open-loop dynamic characteristics of an underactuated system with nonholonomic constraints show the chaotic nature that even though there are small differences in the initial conditions, the amplitude grows to be a completely different movement for its nonlinearity due to centrifugal force, Coriolis force, and gravity. Motions control of underactuated systems known also as nonholonomic control, such as wheeled robot positioning control [41], aerial posture control of space robots [42], and positioning control of underactuated link mechanism robot [43, 44], have been studied. However, a generalized control method has not yet been established to this kind of system due to the difficulties in analysis, and the fact that for an underactuated system, it cannot be directly controlled because of the generalized coordinates of its passive parts. Control methods, which utilize empirical skills catching the motion characteristics and proper motion of the system, are desirable. From the above-mentioned viewpoint, in order to achieve a robot with a high-speed, highly effective optimal motion, and the skill in movement seen in living creatures, fundamental researches on the control method and the trajectory planning method using periodic free movement for the linkage with a passive joint have been done [45–51].
The remaining parts of this chapter are organized as follows: Section 2 discusses a kind of DFC method, PDFC, for a two-link gymnastic robot by using of a Poincaré section. In the Section 3, an improved DFC method, Multiprediction Delayed Feedback Control, is extended to a four-link gymnastic robot. Section 4 gives some numerical simulations to show the effectiveness of the proposed method. Section 5 summarizes the chapter.
The two-link horizontal bar gymnastic robot is a highly simplified model of a human gymnast on a high bar, where the underactuated first joint models the gymnast’s hands on the bar and the actuated second joint models the gymnast’s waist [52]. Studies on such underactuated mechanical systems that possess fewer actuators than degrees of freedom, as gymnastic robot with the underactuated first joint, have received considerable interest in recent years. Open-loop dynamic characteristics of such a linkage as this kind of system, which is classified as an underactuated systems with nonholonomic constraints, show the chaotic nature that even though there are small differences in initial conditions, the amplitude grows to be a completely different movement for its nonlinearity due to centrifugal force, Coriolis force, and gravity. Since a generalized control method has not yet been established to this kind of system for the difficulties in analysis, their control problems are challenging.
While applying the original DFC to the continuous system, such as gymnastic robot, stability analysis of the closed-loop system becomes a very difficult task because of the time-delay dynamics described by a difference-differential equation. A modified delayed feedback control method, called Prediction-based DFC (PDFC), is proposed to stabilize the giant swing motion of two-link horizontal bar gymnastic robot.
The two-link horizontal bar gymnastic robot is a simplified model of a human gymnast on a high bar, where the first joint being passive models the gymnast’s hands on the bar and the second joint comprising of an actuator models the gymnast’s hips. The formula
Two-link gymnastic robot model.
The equation of motion of the gymnastic robot is as follows:
where
In addition, since the first joint cannot generate active torque, the following constraint must be satisfied.
In order to facilitate the analysis, we rewrite the motion Eq. (1) into the following equation of state:
where
Here,
As is known, the stability of a periodic orbit of the original continuous-time system is closely related to the stability of the fixed point of the corresponding Poincaré map. Since the stability of the periodic orbit means that the sequence of points converges to a fixed point in the phase plane, the objective system can be expressed as the following difference equation for the discrete-time systems.
where
Here, let
As shown in Figure 2, the (
Poincaré map of a closed periodic trajectory.
This relationship representing this state transfer map is defined as
If given an initial condition from which a periodic orbit can be formed, it will return to the same point after one period. Hence, the following equation holds:
While a difference occurs in the initial state, the motion will stray away from the periodic orbit. Suppose that there was an error corresponding to
Using the above equation’s Taylor series at
It is obvious that ∂
Now consider the objective system given by the equation of motion in Eq. (3). First, given the case of
Assume the state
where
Moreover, the periodic solution satisfies the following equation.
Here, a Poincaré map is defined as follows:
Then, the T-period sequence of points obtained by sampling the trajectory from the initial value
Thus, it is obvious that the periodic solution is shown as a point passing through the same position. Substituting
Next, taking differentiation for Eq. (16) at
The differential order of the left-hand side in the above equation can be modified as
Thus, it becomes a time-varying linear matrix differential equation with the form of
which is called the Variational Equation if defining
where
Note that
Note that Eq. (3) can be rewritten into
where
The input torque
where
Here,
Since the solution of equation at
Define a Poincaré map as follows:
The continuous system described by Eq. (21) will change to be a discrete system by mapping of
It is obvious here that by taking differentiation at
The differential order of left-hand side in the above equation can also be changed.
Let
Note that
Since the value of both the matrices
Consider the following Prediction-based feedback control:
where
Here
Therefore, the closed-loop system can be described by
Let
then the stabilization problem is reduced as follows:
Given a system Eq. (5), find a feedback gain
By using the pole placement technique, it is not difficult to obtain the value of
Thus, the above design procedure can be applied to analyze the stability of two-link gymnastic robot system.
As is known, the simplified two-link robot shown in the previous section is not an ideal physical model of a human gymnast on a high bar. In order to mimic gymnastic routine more realistically, the complicated robot model with higher degrees of freedom (DOF) needs to be considered. An improved method based on PDFC which control a three-link gymnastic robot via a periodic gain has been proposed [51]. In this section, the Multiprediction Delayed Feedback Control (MDFC), is extended to a more complicated gymnastic robot with four DOF.
Figure 3 shows a four-link horizontal bar gymnastic robot model, which consists of four links and four joints. Shoulder, hips, and knees are active, while the pivot connecting the hand and bar is a passive joint. Assume
Four-link gymnastic robot model.
The motion equation of the robot is
where
Similar to the motion equation of the two-link robot, Eq. (36) can be rewritten as follows.
As is discussed in the reference [51], multiprediction delayed feedback control is based on a new discretization method to increase the control performance by introducing a notion of plural Poincaré maps, (
Image of Poincaré maps [
By the
where
Here, the equivalence of
where rect(
Here,
Summarize the state variables at each one of the
Therefore, the close-loop system relating to X(
where,
As it is known, the local stability of such a system can be determined by the eigenvalue of the monodromy matrix as follows:
The solution of the error transfer matrix
Here, let
Consider the objective system given by the equation of motion in Eq. (37). First, given the case of
Assume the state
where
the variational equation becomes
where
Note that the following equation holds.
Carrying out integrals in numerical integration to the variational equations of Eq. (50) over the interval
Note that Eq. (37) can be rewritten as
Here,
Since the solution of equation at
The continuous system described by Eq. (53) will change to be a discrete system by mapping of
Here, consider the case of differentiation at
Let
Similar to previous subsection, Eq. (53) is equivalent to the following equation.
Note that the following equation holds.
Carrying out integrals in numerical integration to Eq. (58) over the interval
This section shows some simulation results to verify the validity of the proposed prediction-based delayed feedback control for a two-link horizontal bar gymnastic robot. The parameters of the robot model are shown in Table 1. The stability of giant swing motion with period
1st link | 2nd link | ||
---|---|---|---|
Mass | 39.5 | 20.7 | |
Moment of inertia | 3.25 | 1.56 | |
Link length | 1.2 | 0.879 | |
Offset of mass center | 0.756 | 0.377 |
Link parameter values.
By means of the shooting method, an initial condition was obtained such as
Phase portrait of
To see the stability of periodic orbits via delayed feedback control, numerical simulation was exercised from the initial condition
Phase portrait of
Next, consider the system controlled by the proposed method, with which the error transfer matrix
The input matrix
Based on the value of the matrix
In Case I, the numerical simulation results via calculated feedback gain
Phase portrait and joint torque (Case I): (a) Phase portrait of
However, on further analysis conducted, it was found that one can change the type of giant swing type by changing the pole placement of the matrix
Phase portrait and joint torque (Case II): (a) Phase portrait of
It could be said that in Case II, the proposed method showed the ability of controlling the giant-swing motion to an unknown periodic orbit, which is known as one of the advantages of delayed feedback control.
Moreover, Figures 9 and 10 depict the stick diagrams based on one period data after the giant swing motion converging to a stable orbit in Case I and Case II, in which the figures of (a), (b), and (c) plot, respectively, the angle, angular velocity, and stick figure. From the above two figures, it can be seen that the difference between the assigned poles caused periodic giant swing motion to change to a different type.
Angle, angular velocity, and stick (Case I).
Angle, angular velocity, and stick (Case II).
This chapter studied the behavior of the giant swing motions of a two-link and a four-link horizontal bar gymnastic robot systems via delayed feedback control. First, it has been discussed as a solution to calculate analytically the error transfer matrix and the input matrix by which the stability of DFC for the original two-link gymnastic robot system is equivalent to that of the corresponding discrete system. Moreover, it was introduced as a method by which a feedback gain to ensure the stability of original system can be determined. Second, a modified DFC method, MDFC, has been extended to a four-link gymnastic robot. Plural Poincaré maps were defined so that the stability of the close-loop system can be evaluated based on the theory of monodromy matrix. Finally, the simulation results showed its effectiveness.
This work is supported in part by the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and in part by the Scientific Research Foundation for Young Teachers, Shanghai JiaoTong University.
Pesticides are an extensive range of materials to destroy, control, and protect plants from any pest, due to the wide range of applications of pesticides in agriculture, industry, and households.
They are the most common chemical that people have a risk of exposure to them. There are two groups of pesticides based on their origins: chemical pesticides and biological pesticides. Chemical pesticides act nonspecific and affect many off-target organisms, while biopesticides operate host-specific. Various pesticides are shown based on their origin or target insect and function [1]. Almost 2 million tons of pesticides are used worldwide each year, overgrowing. Pesticides directly or indirectly contaminate air, water, soil, and entire ecosystems, posing a severe threat to the health of living things [2, 3].
At present, just about 2 million tons of pesticides are used worldwide, and 47.5% belong to herbicides, 29.5% to insecticides, 17.5% to fungicides, and 5.5% to other pesticides [4]. Pesticides can enter the body in different ways, such as through direct contact, digestion, or inhalation. As pesticides enter the body, they enter the blood circulation and the entire body.
Pesticides may enter life forms totally in different ways. Due to contrasts in the digestion system and other characteristics, species, strains, and individuals may significantly change their defenselessness to pesticides. Oceanic life forms may retain chemicals specifically from the water over respiratory organs (e.g., gills), the body surface, or utilize admissions of sullied nourishment, suspended particles, or sediments [5].
The overall passing and constant illnesses because by pesticide harm number around 1 million every year. The pesticide’s high gamble bunches incorporate creation laborers, formulators, sprayers, blenders, loaders, and rural homestead laborers. During production and formulation, the chance of perils might be higher because the cycles implied are not hazard-free. In modern settings, laborers are at expanded hazard since they handle harmful synthetics, including pesticides, poisonous solvents, and inactive transporters [6].
The human well-being impacts of pesticides can happen through one of three courses: ingestion, inward breath, and skin contact that occur through pesticide items. Microorganisms in water and soil are the primary natural system of pesticides debasement. In contrast, the moment is the pesticide’s digestion system when living life forms expend it as the portion of their nourishment taken up [7].
The sum of the chance of pesticide introduction depends on the harmfulness and the opening of the pesticide. Harmfulness may be a degree of how destructive or harmful a pesticide is (causing sickness or other undesirable impacts). In contrast, the introduction may be a degree of contact (length) with a pesticide. The poisonous quality is measured as a deadly dosage (LD50). The LD50 esteem is the factual assessment of a pesticide (mg/kg of body weight) that can murder 50% of the test creatures within an expressed period (24 hours to 7 days). The LD50 esteem moreover depends on the course of section of a pesticide; oral LD50 for oral ingestion, dermal LD50 for skin contact introduction, and deadly concentration (LC50) for the inward breath of fumigants and pesticide vapors [8].
Because blood is the body’s central circulatory system that can transport a variety of substances to the organs; therefore, it is essential to understand the effects of pesticides on blood and hemoglobin. Investigating the effects of pesticides on biopolymers can help to understand the molecular mechanisms and the hazardous effects of these compounds. Therefore, this chapter aims to study the molecular impact of pesticides.
Pesticides can affect enzyme actions and metabolic pathways related to the whole-cell function (Figure 1) [9].
Pesticides: Formulates, distribution pathways, and impacts on human well-being [
A few pesticides can essentially diminish the action of NADH-dehydrogenase—the fundamental compound of the mitochondrial electron transport chain. The weakness of NADH-dehydrogenase movement by chlorpyrifos might intervene oxidative stress and neurotoxicity. In addition, pesticides can induce the generation of reactive oxygen species (ROS) and receptive nitrogen species (RNS) in cells, which at last prompts oxidative stress and harm to cell structures. Moreover, increased ROS/RNS in vertebrates during digestion and biotransformation of poisonous substances caused hepatotoxicity [9].
Low degrees of pesticides might create an assortment of biochemical changes, some of which might be answerable for the antagonistic organic impacts announced in people and creatures. The harmfulness of pesticides could influence biological organ capacities and biochemical dysfunctions. It was reported a nephrotoxic change in specialists’ occupational exposure to pesticides. Changed liver enzymes, like serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST), have been accounted for among pesticide laborers presented to pesticides [10].
The interaction between different pesticides may result in numerous responses, depending on contrasts within each compound’s chemical properties and modes of poisonous activity. For an improved understanding of the toxic quality of pesticide blends, it is essential to have enough knowledge of the chemical reactivity, the toxicokinetics, metabolic pathways, and the components of activity of each compound. An epidemiological view of physiologically based toxicokinetic and toxicodynamic models, factual modeling, and computational (in silico) toxicology approaches can be used to assess toxicological intelligence [11].
Toxicity effects of pesticides can happen on the activation mechanism of the enzymes at low concentrations; for instance, methyl parathion which is broadly utilized in rural fields, inhibits carbonic anhydrase (CA) and bovine erythrocytes demonstrating that fish in natural and cultural environments are powerless to this pesticide and that methyl parathion pollutions would cause fish and bovine deaths [12].
A few pesticides could regulate the action of efflux carriers or compounds engaged with xenobiotic digestion, prompting an adjustment of the bioavailability and toxicity of other xenobiotics.
Pesticides directly impact a few cell processes and essential proteins engaged with general digestion, cell development, differentiation, and endurance [13].
Moreover, specialists showed a positive connection between openness to pesticides and improvement of certain diseases, especially the brain, prostate, kidney malignant growths, and NHL and leukemia. Some of the examinations on kids observed expanded hazards of illness related to primary times of openness, pre-birth and post-pregnancy, and parental openness at work. Many studies showed developed danger and dose-response connections [14].
Different studies show cytotoxicity of the most commonly used pesticides in agriculture on human cell lines. In 2014, Mesnage et al. designed a toxicity test with nine pesticides on three cell lines, including HepG2, HEK293, and JEG3. The results agreed with cytotoxicity after 24 treatments with pesticides by assessing apoptosis and necrosis [15].
Also, similar results were seen by exposing prostate epithelial WPM-Y.1 cell line with imidacloprid and herbicide glyphosate in the study of Abdel-Halim and Osman [16].
Moreover, the study by Abhishek et al. showed the toxicity of parathion methyl (PM) and carbofuran (CN) pesticides on human keratinocyte (HaCaT) cell lines through MTT assay [17].
Besides the effect of pesticides on cells, metabolic systems, and others, still few studies have been done in vitro and in silico analysis that demonstrates these chemical pesticides have a damaging outcome in genetic and epigenetic alterations, specifically on people who are exposed to them by DNA methylation and miRNA expression [18].
DNA methylation status and miRNAs’ over-expression are linked to crucial cell and molecular pathways, leading to different human diseases [19].
Genetic susceptibility has accounted for modulating the degree of genotoxic hazard. Many investigations have shown a relationship between DNA harm and glutathione-S-transferase polymorphisms [20].
It was known that oxidative stresses because of ROS created by pesticides disrupt DNA and its repair instrument, prompting transformation and illnesses. DNA fix components help to correct the DNA harm brought about by pesticides. Hard well-being impacts because of pesticides range from intense to constant sicknesses, for example, malignant growth, birth absconds, neurological imperfections, reproductive deformities, and immunotoxicity [21].
Oxidative stress is a potential mechanism of toxicity that assumes a critical part in the toxicological pathway of various classes of pesticides, most likely because of their digestion or mitochondrial interruption [22].
In explaining the destructive effect of pesticides on proteins, extensive experiments have been performed on the interaction of pesticides with hemoglobin as a vital protein.
TEPP1 can dissolve REC and enter red blood cells; also, it can interact with the heme prosthetic group (internal) and induce heme degradation when interacting with Hb.2
LIGPLOT analysis of this interaction (Figure 2a) shows that TEPP interacts hydrophobically with aromatic moieties. Docking studies also confirmed the penetration of TEPP into the hydrophobic pocket (Figure 2b). The increase in hydrophobicity around aromatic moieties induces a redshift of the globin moiety, as seen in the results of UV-Vis experiments (Figure 3).
(a) Ligplot analysis of the interaction between TEPP and hemoglobin [
Red shift in globin region of hemoglobin UV-Vis spectrum due to interaction with TEPP [
The blue shift of the Soret (Figure 4) band observed is a result of the action of the pesticide on the hydrophobic pocket of Hb. Negative values of Gibbs free energy indicate spontaneous binding of TEPP to Hb. Oxygen affinity measurements and fluorescence studies have shown that this is due to the interaction of TEPP with Hb.
Blue shift in Soret band of hemoglobin UV-Vis spectrum due to interaction with TEPP [
The concentrations of Hb variants (i.e., deoxy-Hb and metHb) increased and [oxyHb] decreased, suggesting that the oxygen transport capacity of Hb was reduced due to the formation of heme degradation products (Figure 5). The ATR-FTIR study showed that tetraethyl pyrophosphate could alter the secondary structure of hemoglobin by reducing the alpha-helix content [23].
Formation of different hemoglobin variants due to interaction with Hb [
Carbamate insecticide has a lethal effect on the structure and function of human hemoglobin. As shown in (Figure 6a–c), changes in Hb absorption were observed in the 200–700 nm range in various concentrations of Cartap.
(a) UV-Vis spectral changes and red shift in globin region due to interaction with different concentrations of Cartap hydrochloride with hemoglobin [
In addition, the absorption intensity increased at 280 nm, and a bathochromic effect was observed due to the interaction of Cartap and globin through hydrophobic interaction and the change in shape, which was confirmed by molecular docking analysis. In addition, a decrease in the Soret band and Q peak was observed, indicating the affinity of hemoglobin for oxygen in the presence of turbulence in the heme medium and Cartap.
This study shows that the Cartap cause loses standard functionality and negatively affects oxygen affinity and transport. Also, based on thermodynamic analysis (Figure 7), HB’s stability is reduced in the presence of Cartap. According to the molecular docking, Hb has two binding sites Cartap hydrochloride (Figure 8), and is effectively related to proteins through hydrogen bonding and pocket residual water keys. According to the results, hydrogen bonding and hydrophobic interactions play an essential role in the interaction of Cartap with HB, which can denature protein structures.
Thermal denaturation of hemoglobin upon interaction with different concentrations of Cartap hydrochloride [
Docking 3D model of the interaction between Cartap and hemoglobin [
These results show that the interaction of Cartap and hemoglobin results in structural and functional changes in hemoglobin and porphyrin [24].
Titration experiments showed that the fluorescence intensity of the BSA gradually decreased while the fluorescence intensity of the reaction system containing BHb3 increased gradually due to interaction with cypermethrin. The maximum emission wavelength was constant at around 340 nm. That is, there was no red or blue shift. Finally, Chlorpyrifos and Cypermethrin were able to bind BSA and Bovin Hb, and both pesticides bind to Albumine much more potent than that hemoglobin [25].
The reactivity of the heme center with the superoxide anion formed by paraquat is judged by the decrease in the Soret band, and all four heme groups associated with hemoglobin are damaged and eventually destroyed by the superoxide anion formed by the PC.
UV/Vis absorption and synchronous fluorescence spectroscopy revealed that the environmental structure of these Trp (tryptophan) residues was altered and that the results showed that the presence of one class of binding sites on BHb, hydrophobicity, and electrostatic interactions play an essential role in the stabilization of the complex [26].
Ding et al. [27] investigated the binding of Imidacloprid with hemoglobin. They showed that Imidacloprid quenched hemoglobin’s intrinsic fluorescence via the static quenching process. The values of enthalpy (ΔH = −14.58 kJ mol−1) and entropy (ΔS = 32.83 J mol−1 K−1) of the reaction indicate that hydrophobic interactions and hydrogen bonding are the dominant intermolecular forces in stabilizing the Imidacloride-Hb complex.
There are two mechanisms for insecticide resistance: behavioral and physiological. In behavioral resistance, the insect’s reaction reduces or prevents exposure that can lead to death. Otherwise, there are different types of modification mechanisms in physiological resistance like decreasing cuticular penetration and target site sensitivity or increasing metabolic detoxification [28]. To explain more, a common feature of insecticide metabolic resistance is the overexpression of detoxification genes at the transcriptional level, leading to high levels of protein and enzymatic activity. Therefore, this detoxification and resistance development level [29]. One of the notable examples of physiological resistance is malaria, which still exists in some African countries like Tanzania, while chemical insecticides are used against them. The straightforward reason is that target-site insensitivity (knockdown resistance’ target-site mutations) in malaria vectors, lower penetration, or an enhanced detoxification activity [30].
Biopesticides are usually happening compounds or agents acquired from creatures, plants, and microorganisms like microbes, cyanobacteria, and microalgae and are utilized to control farming nuisances and pathogens. There are many kinds of biopesticides, and they are arranged by their extraction sources and the sort of molecule/compound utilized for their readiness. The classifications are microbial pesticides, biochemical pesticides, insect pheromones, plant-based extracts, essential oils, insect growth regulators, and hereditarily adjusted creatures (GMOs) [31].
A proficient observing framework that consistently tests food things for pesticide residues, is a solid motivation for framers to utilize synthetic compounds carefully. Except if defiled shipments can be distinguished, ranchers may not know or care whether the products they are selling contain pesticide residues. Notwithstanding, the offices required for compound testing are costly, while there is some debate over the precision of the less expensive bioassay technique. One promising methodology is HACCP—Hazard Analysis at Critical Control Points. This gander at the entire chain of pesticide conveyance and use and chooses the specific places where activity is plausible and will influence [32]. Figure 9 described the requirements for chemical pesticides to be accepted and used in the market [33].
Stages for confirming a pesticide in the market [
In this chapter, the authors tried to review some of the biological and molecular effects of pesticides on the human body in a few critical ways, from cellular to molecular ones. In the past, insufficient information about the biological effects of chemical substances caused an increase in disease and physical damage. Nowadays, by announcements from international organizations and loading, logical papers agrochemicals more frequently control dangerous bugs and, on a parcel more restricted measure, natural creepy-crawly showers. Despite its viability, the purposeless utilization of chemical pesticides in engaging natural issues causes genuine environmental problems to human well-being, reduces the number of standard adversaries, and gives safe creepy crawlies.
Conversely, biopesticides, utilized for more than a century, are retainers of highlights less significant on the climate and less unsafe to people at any point.
Moreover, biological controlling methods and passing approved and standard processes for manufacturing chemical sprays could be helpful in this way for reducing the consequences of chemical compounds. The study of binding pesticides to proteins is toxicologically essential. This study is expected to provide crucial insights into the interaction of biomacromolecules with pesticides.
There are different molecular assessments of pesticides and their effect on proteins. Still, molecular docking is a well-known program for predicting the interaction of pesticides as ligands and macromolecules like hemoglobin as a target to estimate the penetration of the chemicals on protein pockets. Other analytical assessments, for instance, using spectroscopic methods, could be pretty helpful.
These Terms and Conditions outline the rules and regulations pertaining to the use of IntechOpen’s website www.intechopen.com and all the subdomains owned by IntechOpen located at 5 Princes Gate Court, London, SW7 2QJ, United Kingdom.
',metaTitle:"Terms and Conditions",metaDescription:"These terms and conditions outline the rules and regulations for the use of IntechOpen Website at https://intechopen.com and all its subdomains owned by Intech Limited located at 7th floor, 10 Lower Thames Street, London, EC3R 6AF, UK.",metaKeywords:null,canonicalURL:"/page/terms-and-conditions",contentRaw:'[{"type":"htmlEditorComponent","content":"By accessing the website at www.intechopen.com you are agreeing to be bound by these Terms of Service, all applicable laws and regulations, and agree that you are responsible for compliance with any applicable local laws. Use and/or access to this site is based on full agreement and compliance of these Terms. All materials contained on this website are protected by applicable copyright and trademark laws.
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\n\nThe following terminology applies to these Terms and Conditions, Privacy Statement, Disclaimer Notice, and any or all Agreements:
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\n'}]},successStories:{items:[]},authorsAndEditors:{filterParams:{},profiles:[{id:"396",title:"Dr.",name:"Vedran",middleName:null,surname:"Kordic",slug:"vedran-kordic",fullName:"Vedran Kordic",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/396/images/7281_n.png",biography:"After obtaining his Master's degree in Mechanical Engineering he continued his education at the Vienna University of Technology where he obtained his PhD degree in 2004. He worked as a researcher at the Automation and Control Institute, Faculty of Electrical Engineering, Vienna University of Technology until 2008. His studies in robotics lead him not only to a PhD degree but also inspired him to co-found and build the International Journal of Advanced Robotic Systems - world's first Open Access journal in the field of robotics.",institutionString:null,institution:{name:"TU Wien",country:{name:"Austria"}}},{id:"441",title:"Ph.D.",name:"Jaekyu",middleName:null,surname:"Park",slug:"jaekyu-park",fullName:"Jaekyu Park",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/441/images/1881_n.jpg",biography:null,institutionString:null,institution:{name:"LG Corporation (South Korea)",country:{name:"Korea, South"}}},{id:"465",title:"Dr",name:"Christian",middleName:null,surname:"Martens",slug:"christian-martens",fullName:"Christian Martens",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"479",title:"Dr.",name:"Valentina",middleName:null,surname:"Colla",slug:"valentina-colla",fullName:"Valentina Colla",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/479/images/358_n.jpg",biography:null,institutionString:null,institution:{name:"Sant'Anna School of Advanced Studies",country:{name:"Italy"}}},{id:"494",title:"PhD",name:"Loris",middleName:null,surname:"Nanni",slug:"loris-nanni",fullName:"Loris Nanni",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/494/images/system/494.jpg",biography:"Loris Nanni received his Master Degree cum laude on June-2002 from the University of Bologna, and the April 26th 2006 he received his Ph.D. in Computer Engineering at DEIS, University of Bologna. On September, 29th 2006 he has won a post PhD fellowship from the university of Bologna (from October 2006 to October 2008), at the competitive examination he was ranked first in the industrial engineering area. He extensively served as referee for several international journals. He is author/coauthor of more than 100 research papers. He has been involved in some projects supported by MURST and European Community. His research interests include pattern recognition, bioinformatics, and biometric systems (fingerprint classification and recognition, signature verification, face recognition).",institutionString:null,institution:null},{id:"496",title:"Dr.",name:"Carlos",middleName:null,surname:"Leon",slug:"carlos-leon",fullName:"Carlos Leon",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Seville",country:{name:"Spain"}}},{id:"512",title:"Dr.",name:"Dayang",middleName:null,surname:"Jawawi",slug:"dayang-jawawi",fullName:"Dayang Jawawi",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Technology Malaysia",country:{name:"Malaysia"}}},{id:"528",title:"Dr.",name:"Kresimir",middleName:null,surname:"Delac",slug:"kresimir-delac",fullName:"Kresimir Delac",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/528/images/system/528.jpg",biography:"K. Delac received his B.Sc.E.E. degree in 2003 and is currentlypursuing a Ph.D. degree at the University of Zagreb, Faculty of Electrical Engineering andComputing. His current research interests are digital image analysis, pattern recognition andbiometrics.",institutionString:null,institution:{name:"University of Zagreb",country:{name:"Croatia"}}},{id:"557",title:"Dr.",name:"Andon",middleName:"Venelinov",surname:"Topalov",slug:"andon-topalov",fullName:"Andon Topalov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/557/images/1927_n.jpg",biography:"Dr. Andon V. Topalov received the MSc degree in Control Engineering from the Faculty of Information Systems, Technologies, and Automation at Moscow State University of Civil Engineering (MGGU) in 1979. He then received his PhD degree in Control Engineering from the Department of Automation and Remote Control at Moscow State Mining University (MGSU), Moscow, in 1984. From 1985 to 1986, he was a Research Fellow in the Research Institute for Electronic Equipment, ZZU AD, Plovdiv, Bulgaria. In 1986, he joined the Department of Control Systems, Technical University of Sofia at the Plovdiv campus, where he is presently a Full Professor. He has held long-term visiting Professor/Scholar positions at various institutions in South Korea, Turkey, Mexico, Greece, Belgium, UK, and Germany. And he has coauthored one book and authored or coauthored more than 80 research papers in conference proceedings and journals. His current research interests are in the fields of intelligent control and robotics.",institutionString:null,institution:{name:"Technical University of Sofia",country:{name:"Bulgaria"}}},{id:"585",title:"Prof.",name:"Munir",middleName:null,surname:"Merdan",slug:"munir-merdan",fullName:"Munir Merdan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/585/images/system/585.jpg",biography:"Munir Merdan received the M.Sc. degree in mechanical engineering from the Technical University of Sarajevo, Bosnia and Herzegovina, in 2001, and the Ph.D. degree in electrical engineering from the Vienna University of Technology, Vienna, Austria, in 2009.Since 2005, he has been at the Automation and Control Institute, Vienna University of Technology, where he is currently a Senior Researcher. His research interests include the application of agent technology for achieving agile control in the manufacturing environment.",institutionString:null,institution:null},{id:"605",title:"Prof",name:"Dil",middleName:null,surname:"Hussain",slug:"dil-hussain",fullName:"Dil Hussain",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/605/images/system/605.jpg",biography:"Dr. Dil Muhammad Akbar Hussain is a professor of Electronics Engineering & Computer Science at the Department of Energy Technology, Aalborg University Denmark. Professor Akbar has a Master degree in Digital Electronics from Govt. College University, Lahore Pakistan and a P-hD degree in Control Engineering from the School of Engineering and Applied Sciences, University of Sussex United Kingdom. Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. 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