1. Introduction
Type I hypersensibility to pollen is an important cause of allergy worldwide. In other types of allergy like the food allergic symptoms or very frequently the oral allergy syndrome (OAS), clear differences between varieties/cultivars of the same or highly-related plant species have been described as regard to the expression of allergens and their allergenic importance.
Pioneer studies were carried out in date palm tree over the later years of the last century (Kwaasi et al 1999, 2000). Such studies indicated that allergenicity to date fruit was a cultivar-specific phenomenon, and laboratory data showed that individual cultivars varied in their number of IgE immunoblot bands. Sera from fruit-allergic as well as pollen-allergic patients recognized common fruit-specific epitopes. Also, there was heterogeneity in patient responses to the different extracts. Nevertheless, a number of common allergens were responsible for cross-reactivity between the cultivars.
Up to date, similar studies have been carried out in an important number of plants, mainly those producing edible fruits like apple (Asero et al 2006; Rur 2007; Matthes and Schmitz-Eiberger 2009; Vlieg-Boerstra et al 2011), peach (Brenna et al 2004; Ahrazem et al 2007; Chen et al 2008), cherry (Verschuren, http://www.appliedscience.nl/doc/Onderzoek_111117 _Martie_Verschuren.pdf), nectarine (Ahrazem et al 2007), tomato (Dölle et al 2011), strawberry (Muñoz et al 2010), and lichy (Hoppe et al 2006) among others, and in seeds like cereals (Nakamura et al 2005), buckwheat (Maruyama-Funatsuki et al 2004) and peanuts (Kang et al 2007; Kottapallia et al 2008).
Numerous analysis have raised the question that pollen grains, similarly to fruits may notably differ among different varieties/cultivars in terms of pollen micromorphology, as well as in their physiological characteristics (e.g. viability, vigour, ability to germinate, compatibility…) (Castro et al 2010; Ribeiro et al 2012), and eventually in their allergenic content. However, literature devoted to the comparison of the pollen allergenic characteristics intra- and inter- varieties is still relatively scarce. This article reviews most of these investigations.
2. Taxonomy of allergenic plants
Excellent reviews have been made as regard to the taxonomical classification of the allergenic plants (Yman1982; Takhtajan 1997; D’Amato et al 1998; Mothes et al 2004; Mohapatra et al 2004; Esch 2004; Radauer et al 2006). Moreover, several broad databases have compiled profuse and well-documented information linking the most relevant plant allergenic sources, the identified allergens and their taxonomical classification. They include Pharmacia (Pharmacia Diagnostics, 2001) and later Phadia/Thermo Fisher Scientific (http://www.phadia.com/en/Allergen-information/ImmunoCAP-Allergens/Allergen-compo nents-list/), the Allergome database of allergenic molecules (Mari et al 2009; http://www.allergome.org/index.php) and the official site for the systematic allergen nomenclature approved by the World Health Organization and International Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub-committee (http://www.allergen.org/index.php). Independently of the widespread presence of cross-reactivity, most allergens are described in these works and databases as characterized in a single species (e.g. rBet v 2 Profilin, Birch=
As regards to pollen allergen analysis, two alternatives, apparently opposite, although somehow complementary strategies are defined:
Mothes et al (2004) analyzed cross-reactivities to pollens of trees of the Fagales order, fruits and vegetables, between pollens of the Scrophulariales and pollens of the Coniferales. They proposed a classification of tree pollen and related allergies based on major allergen molecules instead of botanical relationships among the allergenic sources, suggesting Bet v 1 as a marker for Fagales pollen and related plant food allergies, Ole e 1 as a possible marker for Scrophulariales pollen allergy and Cry j 1 and Cry j 2 as potential markers for allergy to Coniferales pollens. Another work analyzed pollen allergen sequences with respect to protein family membership, taxonomic distribution of protein families, and interspecies variability (Radauer and Breteneder 2006). These authors managed to classify all pollen allergens known to date into a limited number of protein families, and divide them into ubiquitous (e.g. profilins), present in certain families (e.g. pectate lyases), or limited to a single taxon (e.g. thaumatin-like proteins). This approach provides invaluable help in issues like the prediction of cross-reactivity, the design of diagnostic methods and the assessment of the allergenic potential of novel molecules. A similar approach is described by Moreno-Aguilar (2008).
On the other hand, different authors are contributing to define the specific allergenic composition of pollens, going deeper into the taxonomical classification usually observed (this is, characterizing the allergenic composition of pollens at infraspecific level), and abounding into the analysis of pollen allergenic polymorphism. Advantages of such strategy have been outlined before (Alché et al 2007). Diverse examples of this strategy are depicted next.
3. Infraspecific botanical names
In botany, an infraspecific name is that corresponding to any taxon below the rank of species. Such names are constructed based in the use of trinomial nomenclature, regulated by the International Code of Botanical Nomenclature (ICBN) (McNeill et al 2006), which includes: genus name, specific epithet, connecting term indicating the rank (not part of the name, but required), and finally the infraspecific epithet. It is habitual to italicize all three parts of the name, but not the connecting term. Five different taxonomical ranks below the species are explicitly allowed in the ICBN:
subspecies - recommended abbreviation: subsp. ("ssp." also widely used)
varietas (variety) - recommended abbreviation: var.
subvarietas (subvariety) - recommended abbreviation: subvar.
forma (form) - recommended abbreviation: f.
subforma (subform) - recommended abbreviation: subf.
The term cultivar is defined as a plant or group of plants selected for desirable characteristics that can be maintained by propagation (http://en.wikipedia. org/wiki/Cultivar). Most cultivars have been obtained after using agronomical methods, or in some cases, selected from wild populations. Crops and even trees used in forestry are usually cultivars that have been selected for desirable characteristics including improved production, resistance to pests, flavor, timber production etc. Naming of cultivars is recommended by the International Code of Nomenclature for Cultivated Plants (ICNCP) (Brickell et al, 2009), and is formed of the scientific botanical name (Latin) followed by the term “cultivar” (usually abbreviated as “cv.”) and a cultivar epithet bounded by single quotation marks, for example:
The terms “cultivar” and “variety” are not equivalent. Although different, both terms are often used as synonyms: thus, "grape varieties" are habitually used in viticulture nomenclature to indicate what should be in reality cultivars, according to the International Code of Nomenclature for Cultivated Plants, since grapes are mostly propagated by cuttings. The same applies to “olive varieties”, which should be properly named “olive cultivars”. In both, and in many other cases, cuttings are the most frequently selected propagation method, as agronomical, physiological and anatomical properties are not maintained in a stable-manner under sexual reproduction. However, usage of the term variety is well fixed in both viticulture and oliviculture, therefore, a change to the correct term (cultivar) is unlikely to occur.
Finally, the term cultigen represents to a plant that has been deliberately altered or selected by humans. It is therefore the result of artificial (anthropogenic) selection. Their naming and origin can be very varied, as it is subjected to different rules and criteria (http://en.wikipedia.org/wiki/Cultigen).
4. Pollens with described differential allergenicity within infraspecific taxonomical ranks
Up to date, the presence of differential allergenicity within infraspecific taxonomical ranks has been demonstrated in the pollen of a significant number of plant species at the allergenic context. Next, we describe pollen allergens in these plants, as well as the most representative literature describing such differences.
4.1. Date palm (Phoenix dactilifera L.)
The most relevant allergenic questions regarding this plant are compiled in the following web pages: http://www.phadia.com/en/Allergen-information/ImmunoCAP-Allergens/Food-of-Plant-Origin/Fruits/Date/ (Phadia), and http://www.allergome.org/script/dettaglio.php? id_molecule=1925 (Allergome).
Briefly,
Kwaasi et al (1994) compared pollen crude extracts from ten cultivars of this tree for their antigenic and allergenic potentials. The results of the tests performed on 6 confirmed atopic patients, including skin prick tests, ELISA, IgG and IgE immunoblotting analyses, peripheral blood lymphocyte proliferation and concomitant interleukin-4 (IL-4) production indicated sharp inter-cultivar heterogeneity. One of the cultivars even failed to elicit any skin test reactivity or bind IgE in atopic sera as determined by the indicated assays. The authors therefore suggest that the antigenicity and allergenicity of date palm pollen is more of a cultivar-specific phenomenon than a species-specific phenomenon, which is governed by the number, quantities or both of the major allergen epitopes possessed by that variety or cultivar. Nevertheless, a number of common allergens are responsible for cross-reactivity between the cultivars.
It has been later demonstrated that antigens and allergens of date fruits cross-react with date pollen allergens and date fruit-sensitive as well as date pollen-allergic patients' sera recognize the same group of date fruit IgE-binding components (Kwaasi et al 1999). Therefore, the cultivar issue is also tremendously important in selecting date cultivars for allergen standardization (Kwaasi et al 2000).
4.2. Arizona cypress (Cupressus arizonica L.)
The most relevant allergenic questions regarding this plant are brought together in the following web pages: http://intapp3.phadia.com/en/Allergen-information/ImmunoCAP-Allergens/Tree-Pollens/Allergens/Arizona-cypress-/ (Phadia), and http://www.allergome. org/ script/dettaglio.php?id_molecule=1793 (Allergome).
In brief:
In general, species of the
Shahali et al (2007) performed a comparative study of the pollen protein contents in two major varieties of
4.3. Birch (Betula verrucosa , Synonym: B. pendula )
Relevant allergenic information concerning this plant (one of the best characterized allergenic sources up to date) is listed in the next web pages: http://intapp3.phadia.com/en/Allergen-information/ImmunoCAP-Allergens/Tree-Pollens/ Allergens/Common-silver-birch-/http://intapp3.phadia.com/en/Allergen-informtion/Imm uno CAP-Allergens/Tree-Pollens/Allergens/Arizona-cypress-/ (Phadia), and http://ww w. allergome.org/script/dettaglio.php?id_molecule=1741 (Allergome).
In short: Birch pollen contains at least 29 antigens (Wiebicke et al 1987). Allergens of molecular weights of 29.5, 17, 12.5, and 13 kDa have been isolated (Florvaag et al 1988; Hirschl, 1989). The following allergens have been characterized: Bet v 1, a 17 kDa protein displaying ribonuclease activity and characterized as a PR-10 protein (Breiteneder et al 1989; Elsayed et al 1990; Grote et al 1993; Scheiner, 1993; Swoboda et al 1994; Taneichi et al 1994; Bufe et al 1996; Holm et al 2001; Mogensen et al 2002; Vieths et al 2002), Bet v 2, a 15 kDa profilin (Elsayed and Vik, 1990; Valenta et al 1991a,b,c; Grote et al 1993; Scheiner, 1993; Seiberler et al 1994; Wiedemann et al 1996; Engel et al 1997; Domke et al 1997; Fedorov et al 1997; Vieths et al 2002), Bet v 3, a 24 kDa calcium-binding protein (Seiberler et a. 1994; Tinghino et al 2002). Bet v 4, a 9 kDa Ca-binding protein (Engel et al 1997; Twardosz et al 1997; Ferreira et al 1999; Grote et al 2002), Bet v 5, a 35 kDa isoflavone reductase-related protein (Vieths et al 1998; Karamloo et al 1999; Stewart and McWilliam, 2001), Bet v 6, a 30-35 kDa protein, PCBER (Phenylcoumaran benzylic ether reductase) (Karamloo et al 2001), Bet v 7, a 18 kDa protein, characterized as a cyclophilin (Cadot et al 2000) and Bet v 11 (Moverare et al 2002).
A large number of these allergens have been expressed as recombinant proteins, including rBet v 1 (Ferreira et al 2003), rBet v 2 (Valenta et al 1991a-c; Niederberger et al 1998; Susani et al 1995; Valenta et al 1993), rBet v 3 (Valenta et al 1991a-c; Seiberler et al 1994), rBet v 4 (Engel et al 1997; Twardosz et al 1997; Ferreira et al 1999), rBet v 5 (Karamloo et al 1999) and rBet v 6 (Vieths et al 2002).
As significant allergenic behaviors, Bet v 1 displays a considerable degree of heterogeneity and consists of at least 20 isoforms which differ in their IgE-binding capacity (Bet v 1a to Bet v 1n), (Breiteneder et al 1989; Elsayed and Vik, 1990; Karamloo et al 1999; Friedl-Hajek et al 1999). Birch pollen-allergic individuals may not be sensitized to any of the major birch pollen allergens.
Evidence of cross-reactivity of birch allergens among different sources is very high: Cross-reactivity exists between pollens from species within the
Selection and breeding of hypoallergenic trees or the application of genetic modification to develop these may potentially reduce the allergenic load caused by birch. This and other objectives have led to the development of studies to characterize genes encoding Bet v 1 isoforms (Schenk et al 2006, 2009). Such studies included the screening of different
A recent publication by Schenk et al (2011) analyzes antigenic and allergenic profiles of pollen extracts from several genotypes of birch species, including several hybrids, and four cultivars of
Schenk et al (2008) reviewed the controversial taxonomy of
4.4. Japanese cedar (Cryptomeria japonica , Synonym: Cupressus japonica )
Relevant allergenic information concerning this plant is assembled in the web pages http://www.phadia.com/en/Allergen-information/ImmunoCAP-Allergens/Tree-Pollens/Allergens/Japanese-cedar-/ (Phadia), and http://www.allergome.org/script/dettaglio. php?id_molecule=1784 (Allergome).
The following allergens have been characterized in this source: Cry j 1, a 45-50 kDa protein, a pectate lyase, is considered a major allergen (Yasueda et al 1983; Taniai et al 1988; Griffith et al 1993; Sone et al 1994; Taniguchi et al 1995; Hashimoto et al 1995; Okano et al 2001; Goto et al 2004; Okano et al 2004; Maeda et al 2005;Takahashi et al 2006; Midoro-Horiuti et al 2006; Kimura et al 2008), Cry j 2, a polygalacturonase, also considered a major allergen (Sakaguchi et al 1990; Namba et al 1994; Komiyama et al 1994; Taniguchi et al 1995; Ohtsuki et al 1995; Futamura et al 2006; Goto-Fukuda et al 2007), Cry j 3, a 27 kDa protein characterized as a thaumatin, and a PR-5 protein (Fujimura et al 2007; Futamura et al 2002, 2006), Cry j 4, a Ca-binding protein (Futamura et al 2006), Cry j IFR, an isoflavone reductase (Kawamoto et al 2002), Cry j, a chitinase (Fujimura et al 2005), Cry j AP, a Aspartic Protease (Ibrahim et al 2010a), Cry j CPA9, a serin protease (Ibrahim et al 2010b), and Cry j LTP, a Lipid Transfer Protein (Ibrahim et al 2010c). Moreover, a number of other antigenic proteins have been isolated but not characterized, including proteins of 7, 15 and 20 kDa (Matsumura et al 2006).
Cross-reactivity among conifer pollens has been documented (Aceituno et al 2000; Midoro-Horiuti et al 1999; Ito et al 1995). This could be explained by the high similarity between the Japanese cedar allergen Cry j 1 and the major allergens of Mountain cedar (Jun a 1), Japanese cypress (Cha o 1) and
Cry j 1 and Cry j 2 are major allergens. However, concentrations of these allergens vary greatly in pollen from different individual Japanese cedar trees (Goto-Fukuda et al 2007). Most basically, there are 2 varieties of Japanese cedar trees: the popular diploid and the less popular triploid. These trees are not very different morphologically. In a comparison of the major allergens Cry j 1 and Cry j 2, the triploid tree pollen extract was shown to have lower concentrations of both. The pollen from this variety may thus be less allergenic (Kondo et al 1997). Conspicuous differences were detected in the presence of the Cry j 1 allergen in two kinds of cultivar: ΄Mio΄ and ΄Masuyama΄ (Saito and Teranishi, 2002).
4.5. Olive tree (Olea europaea L.)
Relevant allergenic information concerning this plant is compiled in the web pages http://www.phadia.com/en/Allergen-information/ImmunoCAP-Allergens/Tree-Pollens/Allergens/Olive-/(Phadia), and http://www.allergome.org/script/dettaglio.php? id_molecule=1888 (Allergome). Furthermore, a very recent article by Esteve et al (2012) reviews the information available about the characterized olive allergens at present, the procedures used for such physicochemical and immunological characterization, as well as for extraction and production of olive allergens. Up to date, twelve allergens have been identified in olive pollen while just one allergen has been identified in olive fruit. Additional reviews on olive pollen allergens include the chapters by Jimenez-Lopez et al, Morales et al, and Zienkiewicz et al included in this book.
Olive pollen is by far the most studied allergenic pollen at infraspecific taxonomical level. An important point to explain this is the fact that the olive germplasm (extremely rich although still unexplored in its totality), is the subject of numerous analysis carried out in order to characterize cultivar identity. These works include the use of morphological traits (Barranco and Rallo, 1984; Cimato et al, 1993; Barranco et al, 2005; Caballero et al, 2006) as well as molecular methods, which started with the use of isoenzyme markers (Ouazzani et al, 1993; Trujillo et al, 1995) and at a later stage have been carried out utilizing DNA markers as RFLPs (Besnard et al 2001), RAPDs (Belaj et al, 2001; Fabbri et al, 1995), AFLPs (Angiolillo et al, 1999) and microsatellite markers (SSRs). SSRs are one of the most reliable methods used in olive cultivar characterization (Baldoni et al, 2009; La Mantia et al, 2005). SSRs markers have been successfully used in germplasm bank classification and contributed to a better management of several olive collections around the world (Khadari et al, 2003; Muzzalupo et al, 2006; Fendri et al 2010). In order to provide a better world-wide applicable tool for olive DNA typing, a list of 11 SSRs markers has been selected among microsatellites available for olive cultivar characterization (Baldoni et al, 2009). These works have led to the publication of different olive cultivar catalogues (Barranco and Rallo 1984; Cimato et al 1993; Barranco et al 2000; Caballero et al 2006).
Earlier evidence of the relationships between olive allergen polymorphism and the cultivar origin of olive pollen was reviewed by Alché et al (2007), with particular reference to the publications available at that time, including those by Barber et al 1990, Geller-Bernstein et al (1996), Waisel and Geller-Bernstein (1996), Castro (2001, 2003), Carnes Sanchez et al (2002), Conde Hernandez et al (2002), Hamman-Khalifa et al (2003, 2005), Alché et al (2003), Napoli et al (2006) and Fernandez-Caldas et al (2007). Further confirmation at the molecular level has risen since, based in the use of powerful cloning, proteomics (peptide mapping and N-glycopeptide analysis) and bioinformatics methods. These include the analysis of numerous cDNA and peptide/glucan sequences from Ole e 1 (Napoli et al, 2008; Hamman Khalifa et al, 2010; Castro et al, 2012; Jiménez-López et al, 2011; Soleimani et al, 2012a,b), Ole e 5 (Zafra, 2007), Ole e 2 (Jiménez López, 2008; Morales et al, 2008; Jiménez-López et al, 2012b), and Ole e 11 (Jiménez-López et al, 2012a). Moreover, the reactivity of a broad panel of olive pollen cultivar extracts to diverse patient’ sera has been also analyzed in Jordan (Jaradat et al, 2011). Recently, a novel multiplex method for the simultaneous detection and relative quantification of pollen allergens has been set up (Morales 2012; Morales et al, 2012). This method will help to investigate pollen allergen polymorphism within cultivars in combination with patient’s reactivity, by notably improving the specificity and capacity of the biochemical and immunological assays. The present book also includes remarkable analyses of olive varietal polymorphism in those chapters by Jimenez-Lopez et al, Morales et al, and Zienkiewicz et al
5. Conclusions and future perspectives
The past and recent developments in the analysis of the differential allergenicity of pollens from heterogeneous infraspecific taxonomic ranks described above, confirm the need of rethinking current strategies for basic research on pollen allergen characterization, and the design of diagnosis and specific immunotherapy approaches. These issues, raised and discussed initially by us (Alché et al, 2007) for olive pollen allergens, seem to be valid for a broader number of species, as stated here. Extensive pollen allergen polymorphism is known to represent a general feature over the plant kingdom. The limitation of the study of this polymorphism just to the level of species represents a restriction which may limit both basic knowledge and more importantly the efficacy and the future development of strategies to detect and contest human pollen allergy. Although the use of marker allergens for order, genera or even plant families may represent an invaluable tool (Mothes et al, 2004), relevant differences in patient’s reactivity occur even among close-related taxonomical ranks (e.g. van Ree 2002; Asero et al, 2005; Fenaille et al, 2009; Wallner et al, 2009a,b; Jaradat et al, 2011) therefore determining that even close allergenic compositions are not always “fully equivalent”. The analysis of allergenic variability in infraspecific taxonomical ranks should be considered a “must” that can be easily incorporated into most developing and evolving trends in allergy analysis and clinics, namely the design of highly specific and personalized natural extracts, hypoallergens, the design and production of recombinant allergens, hybrid molecules, high-throughput diagnosis, new forms of allergen administration and release, the analysis of allergen cross-reactivity etc. (Schenk et al, 2006, 2011; Gao et al, 2008; Wallner et al, 2009a,b).
Agricultural and environmental strategies to reduce the impact of pollen allergy involving the use of differential infraspecific taxonomic ranks are not to be discarded either. They may include the primary screening of relatively less allergenic varieties as proposed for wheat, buckwheat and other food sources (Nair and Adachi, 2002; Nakamura et al, 2005; Spangenberg et al, 2006), and the future design of varieties/hybrids with reduced pollen production, limited period of flowering, or even androsteril characteristics in a similar way of that proposed for the Gilissen et al (2006a,b) for the production of hypoallergenic plant foods by selection, breeding and genetic modifications.
Acknowledgement
This work was funded by ERDF (co)-financed projects P2010-CVI5767, P2010-AGR6274, BFU2011-22779, P2011-CVI-7487 and PEOPLE-IOF/1526.
References
- 1.
Del Pozo, V., Mínguez, A., Arrieta, I., Cortegano, I., Cárdaba, B., Gallardo, S., Rojo, M., Palomino, P. & Lahoz, C. (Aceituno E 2000 Molecular cloning of major allergen from Cupressus arizonica pollen: Cup a 1. ,30 12 1750 1758 - 2.
Di Felice, G., Tinghino, R., Mari, A. & Pini, C. (Afferni C Iacovacci P Barletta B 1999 Role of carbohydrate moieties in IgE binding to allergenic components of Cupressus arizonica pollen extract ,29 8 1087 1094 - 3.
Ahrazem O Jimeno L López-torrejón G Herrero M Espada J. L Sánchez-monge R Duffort O Barber D Salcedo G 2007 Assessing allergen levels in peach and nectarine cultivars. Asthma & Immunology,99 1 42 47 - 4.
Alché J. D Castro A. J Jiménez-lópez J. C Morales S Zafra A Hamman-khalifa A. M Rodríguez-garcía M. I 2007 Differential characteristics of the olive pollen from different cultivars and its biological and clinical implications. ,17 Suppl 1.,63 68 - 5.
Di Felice, G. & Pini, C. (Alisi C Afferni C Iacovacci P Barletta B Tinghino R Butteroni C Puggioni E. M Wilson I. B Federico R Schinina M. E Ariano R 2001 Rapid isolation, characterization, and glycan analysis of Cup a 1, the major allergen of Arizona cypress () pollen. Allergy,56 10 978 984 - 6.
Angiolillo A Mencuccini M. & L Baldoni L 1999 Olive genetic diversity assessed using amplified fragment length polymorphisms ,98 3-4 411 421 - 7.
Arilla M. C Ibarrola I Garcia R De La Hoz B Martinez A Asturias J. A 2004 Quantification of the Major Allergen from Cypress () Pollen, Cup a 1, by Monoclonal Antibody-Based ELISA. International Archives of Allergy and Immunology,134 1 10 16 - 8.
Asero R Marzban G Martinelli A Zaccarini M Machado M. L 2006 Search for low-allergenic apple cultivars for birch-pollen-allergic patients: is there a correlation between in vitro assays and patient response? ,38 3 94 98 - 9.
Asero R Weber B Mistrello G Amato S Madonini E Cromwell O 2005 Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences between short and giant ragweed. ,116 5 1036 1041 - 10.
Baldoni L Cultrera N. G Mariotti R Ricciolini C Arcioni S Vendramin G. G Buonamici A Porceddu A Sarri V Ojeda M. A Trujillo I Rallo L Belaj A Perri E Salimonti A Muzzalupo I Casagrande A Lain O Messina R Testolin R 2009 A consensus list of microsatellite markers for olive genotyping ,24 3 213 231 - 11.
Ballmer-weber B. K Vieths S Luttkopf D Heuschmann P Wüthrich B 2000 Celery allergy confirmed by double-blind, placebo-controlled food challenge: a clinical study in 32 subjects with a history of adverse reactions to celery root The106 2 373 378 - 12.
Di Felice, G. & Pini, C. (Barletta B Afferni C Tinghino R Mari A 1996 Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extracts. 98 4 797 804 - 13.
Barranco D L Rallo 1984 Las variedades de olivo cultivadas en Andalucía. . Madrid. Spain. - 14.
Barranco D A Cimato P Fiorino L Rallo A Touzani C Castaneda F Serafín Trujillo I 2000 . Madrid. España. - 15.
Caballero, C. Del Rio, A. Martin, J. Tous, and I. Trujillo (eds). ,Barranco D Trujillo I L Rallo L 2005 Libro I Elaiografía Hispánica L Rallo D Barranco J. M 45 231 - 16.
Bartel J. A Adams R. P James S. A Mumba L. E Pandey R. N 2003 Variation among Cupressus species from the western hemisphere based on random amplified polymorphic DNAs. ,31 - 17.
Belaj A Trujillo I De La Rosa R Rallo L Giménez M. J 2001 Polymorphism and discriminating capacity of randomly amplified polymorphic markers in an olive germplasm bank. American Society for Horticultural Science,126 1 64 71 - 18.
Besnard G Batadat P Chevalier D Tagmount A Bervillé A 2001 Genetic differentiation in the olive complex (Olea europaea) revealed by RAPDs and RFLPs in the rRNA genes 48 165 182 - 19.
Martin Esteban, M. & Castillo, R. (Blanco C Carrillo T Quiralte J Pascual C 1995 Occupational rhinoconjunctivitis and bronchial asthma due to Phoenix canariensis pollen allergy. 50 3 277 280 - 20.
Bousquet J Knani J Hejjaoui A Ferrando R Cour P Dhivert H Michel F. B 1993 Heterogeneity of atopy. I. Clinical and immunologic characteristics of patients allergic to cypress pollen.48 3 183 188 - 21.
Breiteneder H Ferreira F Hoffmann-sommergruber K Ebner C Breitenbach M Rumpold H Kraft D Scheiner O 1993 Four recombinant isoforms of Cor a I, the major allergen of hazel pollen, show different IgE-binding properties. ,212 2 355 362 - 22.
Breiteneder H Pettenburger K Bito A Valenta R Kraft D Rumpold H Scheiner O Breitenbach M 1989 The gene encoding for the major birch pollen allergen Bet v 1 is highly homologous to a pea disease resistance response gene. ,8 7 1935 1938 - 23.
Brenna O. V Pastorello E. A Farioli L Pravettoni V Pompei C 2004 Presence of allergenic proteins in different peach (Prunus persica) cultivars and dependence of their content on fruit ripening. 52 26 7997 8000 - 24.
Xiaobai Jin, & Cubey, J.J. (Brickell C. D Alexander C David J. C Hetterscheid W. L. A Leslie A. C Malecot V 2009 International code of nomenclature for cultivated plants , Scripta Horticulturae 10, 204 pages. - 25.
Bufe A Spangfort M. D Kahlert H Schlaak M Becker W. M 1996 The major birch pollen allergen, Bet v 1, shows ribonuclease activity. 199 3 413 415 - 26.
del Rio, C., Barranco, D., & Trujillo, I. (Caballero J. M 2006 The Olive World Germplasm Bank of Córdoba, Spain. ,25 14 19 - 27.
Cadot P Diaz J. F Proost P Van D J Engelborghs Y Stevens E. A Ceuppens J. L 2000 Purification and characterization of an 18-kd allergen of birch (Betula verrucosa) pollen: identification as a cyclophilin. 105 2 Pt1),286 291 - 28.
Caiaffa M. F Macchia L Strada S Bariletto G Scarpelli F Tursi A 1993 Airborne Cupressaceae pollen in southern Italy. 71 1 45 50 - 29.
Carnés Sánchez J., Iraola, V.M., Sastre, J., Florido, F., Boluda, L. & Fernandez-Caldas, E. (2002 Allergenicity and immunochemical characterization of six varieties of Olea europaea. 57 4 313 318 - 30.
Castro A. J 2001 Aproximación a la función biológica del alérgeno mayoritario del polen del olivo (Ole e 1). Implicaciones clínicas y ambientales. Doctoral thesis. Granada (Spain): University of Granada. - 31.
Castro A. J Alché J. D Cuevas J Romero P. J Alché V Rodríguez-garcía M. I 2003 Pollen from different olive tree cultivars contains varying amounts of the major allergen Ole e 1 ,131 3 164 173 - 32.
Castro A. J Bednarczyk A Schaeffer-reiss C Rodríguez-garcía M. I Van Dorsselaer A Alché J. D 2010 Screening of Ole e 1 polymorphism among olive cultivars by peptide mapping and N-glycopeptide analysis ,10 5 953 621 - 33.
Castro A. J Rejón J. D Fendri M Jiménez-quesada M. J Zafra A Jiménez-lópez J. C Rodríguez-garcía M. I Alché J. D 2010 Taxonomical discrimination of pollen grains by using confocal laser scanning microscopy (CLSM) imaging of autofluorescence. ,4 607 613 - 34.
Chen L Zhang S Illa E Song L Wu S Howad W Arús P Weg E Chen K Gao Z 2008 Genomic characterization of putative allergen genes in peach/almond and their synteny with apple. 9 543 - 35.
Cimato A Cantini C Sani G Marranci M 1993 II Germoplasma dell’ Olivo in Toscana. Ed. , Florence, Italy.1254 - 36.
Conde Hernández J., Conde Hernández, P., González Quevedo Tejerina, M.T., Conde Alcañiz, M.A., Conde Alcañiz, E.M., Crespo Moreira, P. & Cabanillas Platero, M. Antigenic and allergenic differences between 16 different cultivars of Olea europaea. ,57 Suppl. 71,60 65 - 37.
Del Moral, A., Lopez, E., Lombardero, M., Del Pozo, V., Lahoz, C. (Cortegano I Civantos E Aceituno E 2004 Cloning and expression of a major allergen from pollen, Cup a 3, a PR-5 protein expressed under polluted environment. Acta Allergologica,59 5 485 490 - 38.
allergy in Europe.D Amato G Spieksma F. T Liccardi G Jäger S Russo M Kontou-fili K Nikkels H Wüthrich B Bonini S 1998 Pollen-related 53 6 567 578 - 39.
De Coana Y. P Parody N Fuertes M. A Carnes J Roncarolo D Ariano R Sastre J Mistrello G Alonso C 2010 Molecular cloning and characterization of Cup a 4, a new allergen from Cupressus arizonica 401 3 451 457 - 40.
Di Felice G., Barletta, B., Tinghino, R. & Pini, C. (2001 Cupressaceae pollinosis: identification, purification and cloning of relevant allergens ,125 4 280 289 - 41.
Di Felice G., Caiaffa, M.F., Bariletto, G., Afferni, C., Di Paolab, R., Mari, A., Palumbo, S., Tinghino, R., Sallusto, F., Tursi, A., Macchia, L., Pini, C. (1994 Allergens of Arizona cypress (Cupressus arizonica) pollen: characterization of the pollen extract and identification of the allergenic components ,94 3 Pt 1),547 555 - 42.
Dietschi R Wüthrich B Johansson S. G. O 1987 So-called ”celery-carrot-mugwort-spice syndrome.” RAST results with new spice discs. ,87 62 524 531 - 43.
Dölle S Lehmann K Schwarz D Weckwert W Scheler C George E Franken P Worm M 2011 Allergenic activity of different tomato cultivars in tomato allergic subjects 41 11 1643 1652 - 44.
Domke T Federau T Schluter K Giehl K Valenta R Schomburg D Jockusch B. M 1997 Birch pollen profilin: structural organization and interaction with poly-(L-proline) peptides as revealed by NMR. 411 2-3 291 295 - 45.
Ebner C Birkner T Valenta R Rumpold H Breitenbach M Scheiner O Kraft D 1991 Common epitopes of birch pollen and apples-studies by western and northern blot. 88 4 588 594 - 46.
Elsayed S & Vik, H. (1990 Purification and N-terminal amino acid sequence of two birch pollen isoallergens (Bet v I and Bet v II). 93 4 378 384 - 47.
Engel E Richter K Obermeyer G Briza P Kungl A. J Simon B Auer M Ebner C Rheinberger H. J Breitenbach M Ferreira F 1997 Immunological and biological properties of Bet v 4, a novel birch pollen allergen with two EF-hand calcium binding domains. 272 45 28630 28637 - 48.
Eriksson N. E Wihl J. A Arrendal H Strandhede S. O Tree pollen allergy. III. ( e study.1987 Cross reactions based on results from skin prick tests and the RAST in hay fever patients. A multi-centr42 3 205 214 - 49.
Esch R. E 2004 Grass pollenallergens. Allergens and Allergen Im munotherapy.185 206 - 50.
Esteve C Montealegre C Marina M. L García M. C 2012 Analysis of olive allergens 15 92 1 14 - 51.
Fabbri A Hormaza J. I Polito V. S 1995 Random amplified polymorphic DNA analysis of olive (Olea europaea L.) cultivars 120 1 538 542 - 52.
Fedorov A. A Ball T Valenta R Almo S. C 1997 X-ray crystal structures of birch pollen profilin and Phl p 2 113 1-3 109 113 - 53.
Fenaille F Nony E Chabre H Lautrette A Couret M. N Batard T Moingeon P Ezan E 2009 Mass spectrometric investigation of molecular variability of grass pollen group 1 allergens ,8 8 4014 4027 - 54.
Fendri M Trujillo M Trigui A Rodríguez-garcía M. I Alché J. D 2010 Simple sequence repeat identification and endocarp characterization of olive tree accessions in a Tunisian germplasm collection ,45 10 1429 1436 - 55.
Fernández-caldas E Carnés J Iraola V Casanovas M 2007 Comparison of the allergenicity and Ole e 1 content of 6 varieties of pollen collected during 5 consecutive years. Annals of Allergy, Asthma & Immunology,98 5 464 470 - 56.
Ferreira F Engel E Briza P Richter K Ebner C Breitenbach M 1999 Characterization of recombinant Bet v 4, a birch pollen allergen with two EF-hand calcium-binding domains.118 2-4 304 305 - 57.
Ferreira F. D Hoffmann-sommergruber K Breiteneder H Pettenburger K Ebner C Sommergruber W Steiner R Bohle B Sperr W. R Valent P Kungl A. J Breitenbach M Kraft D Scheiner O 1993 Purification and characterization of recombinant Bet v I, the major birch pollen allergen. Immunological equivalence to natural Bet v I. 68 26 19574 11980 - 58.
Florvaag E Holen E Vik H Elsayed S 1988 Comparative studies on tree pollen allergens XIV. Characterization of the birch () and hazel (Corylus avellana) pollen extracts by horizontal 2-D SDS-PAGE combined with electrophoretic transfer and IgE immunoautoradiography. Annals of Allergy,61 5 392 400 - 59.
Bet v 1 isoforms including a naturally occurring truncated form of the protein derived from Austrian birch pollen.Friedl-hajek R Radauer C O Riordain G Hoffmann-sommergruber K Leberl K Scheiner O Breiteneder H 1999 New 36 10 639 645 - 60.
Fujimura T Shigeta S Suwa T Kawamoto S Aki T Masubuchi M Hayashi T Hide M Ono K 2005 Molecular cloning of a class IV chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen and competitive inhibition of its immunoglobulin E-binding capacity by latex C-serum ,35 2 234 243 - 61.
Fujimura T Futamura N Midoro-horiuti T Togawa A Goldblum R. M Yasueda H Saito A Shinohara K Masuda K Kurata K Sakaguchi M 2007 Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria japonica) pollen.62 5 547 553 - 62.
Futamura N Mukai Y Sakaguchi M Yasueda H Inouye S Midoro-horiuti T Goldblum R. M Shinohara K 2002 Isolation and characterization of cDNAs that encode homologs of a pathogenesis-related protein allergen from Cryptomeria japonica. Biotechnology and Biochemistry,66 11 2495 2500 - 63.
Futamura N Kusunoki Y Mukai Y Shinohara K 2006 Characterization of genes for a pollen allergen, Cry j 2, of Cryptomeria japonica.28 143 59 68 - 64.
L.J.W.J. (Gao Z E. W Weg E. W Matos C. I Arens P Bolhaar S. T. H. P Knulst A. C Li Y Hoffmann-sommergruber K Gilissen 2008 Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity. 116 EOF - 65.
Geller-bernstein C Arad G Keynan N Lahoz C Cardaba B Waisel Y 1996 Hypersensitivity to pollen of Olea europaea in Israel. 51 5 356 359 - 66.
van de Weg, W.E. (Gilissen L. J. W. J Bolhaar S. T. H. P Knulst A. C Zuidmeer L Van Ree R Gao Z. S 2006a Production of hypoallergenic plant foods by selection, breeding and genetic modification In: Allergy matters. New approaches to allergy prevention and management. Gilissen, L.J.E.J., Wichers, H.J., Savelkoul, H.F.J. and Bogers, R.J. (Eds). , Chapter 11,95 105 - 67.
Gilissen L. J. W. J Wichers H. J Savelkoul H. F. J Beers G 2006b Future developments in allergy prevention: a matter of integrating medical, natural and social sciences. In: Allergy matters. New approaches to allergy prevention and management. Gilissen, L.J.E.J., Wichers, H.J., Savelkoul, H.F.J. and Bogers, R.J. (Eds). , Chapter, 1,3 12 - 68.
isoforms derived from trees have different binding properties to monoclonal antibodies. Clinical and Experimental Allergy,Goto Y Kondo T Ide T Yasueda H Kuramoto N Yamamoto K 2004 Cry j 34 11 1754 1761 - 69.
Goto-fukuda Y Yasueda H Saito A Kondo T 2007 Investigation of the variation of Cry j 2 concentration in pollen among sugi ( d. Don) trees using a newly established extraction method. Arerugi,56 10 1262 1269 - 70.
Griffith I. J Lussier A Garman R Koury R Yeung H Pollock J 1993 The cDNA cloning of Cry j 1, the major allergen of Cryptomeria japonica.91 339 - 71.
Grote M Stumvoll S Reichelt R Lidholm J Rudolf V 2002 Identification of an allergen related to Phl p 4, a major timothy grass pollen allergen, in pollens, vegetables, and fruits by immunogold electron microscopy. 83 9 1441 1445 - 72.
Grote M Vrtala S Valenta R 1993 Monitoring of two allergens, Bet v I and profilin, in dry and rehydrated birch pollen by immunogold electron microscopy and immunoblotting. ,41 5 745 750 - 73.
Hamman-khalifa A. M 2005 Utilización de marcadores relacionados con la alergenicidad y la biosíntesis de lípidos para la discriminación entre cultivares de olivo. Doctoral thesis. Granada (Spain): University of Granada. - 74.
Hamman-khalifa A. M Alché J. D Rodríguez-garcía M. I 2003 Discriminación molecular en el polen de variedades españolas y marroquíes de olivo ( L.). Polen.13 219 225 - 75.
Hamman-khalifa A. M Castro-lópez A. J Jiménez-lópez J. C Rodríguez-garcía M. I Alché J. D 2008 Olive cultivar origin is a major cause of polymorphism for Ole e 1 pollen allergen , 8:10. - 76.
Hashimoto M Nigi H Sakaguchi M Inouye S Imaoka K Miyazawa H Taniguchi Y Kurimoto M Yasueda H Ogawa T 1995 Sensitivity to two major allergens (Cry j I and Cry j II) in patients with Japanese Cedar (Cryptomeria japonica) pollinosis. .25 9 848 852 - 77.
Helbling A 1997 Important cross-reactive allergens. ,127 10 382 389 - 78.
Hirschl M. H 1989 Isolation and characterization of birch pollen protein P13. ,101 19 679 681 - 79.
Holm J Baerentzen G Gajhede M Ipsen H Larsen J. N Lowenstein H Wissenbacha M Spangforta M. D 2001 Molecular basis of allergic cross-reactivity between group 1 major allergens from birch and apple B: Biomedical Sciences and Applications,756 1-2 307 313 - 80.
Hoppe S Neidhart S Zunker K Hutasingh P Carle R Steinhart H Paschke A 2006 The influences of cultivar and thermal processing on the allergenic potency of lychees (Litchi chinensis SONN.) 96 2 209 219 - 81.
Ibrahim A. R Kawamoto S Aki T Shimada Y Rikimaru S Onishi N Babiker E. E Oiso I Hashimoto K Hayashi T Ono K 2010a Molecular cloning and immunochemical characterization of a novel major japanese cedar pollen allergen belonging to the aspartic protease family 152 3 207 218 - 82.
Ibrahim A. R Kawamoto S Mizuno K Shimada Y Rikimaru S Onishi N Hashimoto K Aki T Hayashi T Ono K 2010b Molecular cloning and immunochemical characterization of a new japanese cedar pollen allergen homologous to plant subtilisin-like serine protease 3 1 262 265 - 83.
Ibrahim A. R Kawamoto S Nishimura M Pak S Aki T Diaz-perales A Salcedo G Asturias J. A Hayashi T Ono K 2010c A new lipid transfer protein homolog identified as an IgE-binding antigen from japanese cedar pollen Biotechnology, and Biochemistry,74 3 504 509 - 84.
Nuchel Petersen, B., Munch, E.P., Wihl, J.A. & Løwenstein, H. (Ipsen H Bowadt H Janniche H 1985 Immunochemical characterization of reference alder () and hazel (Corylus avellana) pollen extracts and the partial immunochemical identity between the major allergens of alder, birch and hazel pollens. Allergy,40 7 510 518 - 85.
Ito H Nishimura J Suzuki M Mamiya S Sato K Takagi I Baba S 1995 Specific IgE to Japanese cypress (Chamaecyparis obtusa) in patients with nasal allergy Asthma & Immunology.74 4 299 303 - 86.
Al Bzourb, A., Ababnehac, Q., Shdiefatd, S., Jaradatb, S. & Al Domie, H. (Jaradat Z. W 2012 Identification of allergenic pollen grains in 36 olive (Olea europaea) cultivars grown in Jordan 23 3 255 264 - 87.
Jimenez-lopez J. C 2008 Caracterización molecular del polimorfismo de las profilinas en el polen del olivo y otras especies alergogénicas. Doctoral thesis. Granada (Spain): University of Granada. - 88.
and functional features of olive pollen pectin methylesterase using homology modeling and molecular docking methods. . In press.Jimenez-lopez J. C Kotchoni S. O Rodríguez-garcía M. I Alché J. D 2012a Structure - 89.
Jimenez-lopez J. C Morales S Castro A. J Volkmann D Rodríguez-garcía M. I Alché J. D 2012b Characterization of profilin polymorphism in pollen with a focus on multifunctionality ,7 2 e30878. - 90.
Jimenez-lopez J. C Rodríguez-garcía M. I Alché J. D 2011 Systematic and Phylogenetic Analysis of the Ole e 1 Pollen Protein Family Members in Plants. In: Systems and Computational Biology- Bioinformatics and Computational Modeling, Ning-Sun Yang (Ed.), ,245 260 - 91.
Jung K Schlenvoigt G Jäger L 1987 Allergologic-immunochemical study of tree and bush pollen. III- Cross reactions of human IgE antibodies with various tree pollen allergens.33 4 223 230 - 92.
Kang I. H Srivastava P Ozias-akins P Gallo M 2007 Temporal and spatial expression of the major allergens in developing and germinating peanut seed. 144 2 836 845 - 93.
Karamloo F Schmitz N Scheurer S Foetisch K Hoffman A Haustein D Vieths S 1999 Molecular cloning and characterization of a birch pollen minor allergen, Bet v 5, belonging to a family of isoflavone reductase-related proteins. 104 5 991 999 - 94.
Karamloo F Wangorsch A Kasahara H Davin L. B Haustein D Lewis N. G Vieths S 2001 Phenylcoumaran benzylic ether and isoflavonoid reductases are a new class of cross-reactive allergens in birch pollen, fruits and vegetables 268 20 5310 5320 - 95.
Kawamoto S Fujimura T Nishida M Tanaka T Aki T Masubuchi M Hayashi T Suzuki O Shigeta S Ono K 2002 Molecular cloning and characterization of a new Japanese cedar pollen allergen homologous to plant isoflavone reductase family ,32 7 1064 1070 - 96.
Khadari B Breton C Moutier N Roger J. P Besnard G Bervillé A Dosba F 2003 The use of molecular markers for germplasm management in a French olive collection. ,106 3 521 529 - 97.
Kimura Y Kuroki M Maeda M Okano M Yokoyama M Kino K 2008 Glycoform analysis of Japanese cypress pollen allergen, Cha o 1: a comparison of the glycoforms of cedar and cypress pollen allergens Biotechnology, and Biochemistry,72 2 485 491 - 98.
Komiyama N Sone T Shimizu K Morikubo K Kino K 1994 cDNA cloning and expression of Cry j II the second major allergen of Japanese cedar pollen. 201 2 1021 1028 - 99.
Kondo Y Ipsen H Lowenstein H Karpas A Hsieh L. S 1997 Comparison of concentrations of Cry j 1 and Cry j 2 in diploid and triploid Japanese Cedar (Cryptomeria japonica) pollen extracts. 52 4 455 459 - 100.
Kondo Y Tokuda R Urisu A Matsuda T 2002 Assessment of cross-reactivity between Japanese cedar (Cryptomeria japonica) pollen and tomato fruit extracts by RAST inhibition and immunoblot inhibition Clinical and Experimental Allergy,32 4 590 594 - 101.
Kos T Hoffmann-sommergruber K Ferreira F Hirschwehr R Ahorn H Horak F Jäger S Sperr W Kraft D Scheiner O 1993 Purification, characterization and N-terminal amino acid sequence ofa new major allergen from European chestnut pollen- Cas s 1. Biochemical and Biophysical Research Communications,196 3 1086 1092 - 102.
Kottapallia K. R Paytonb P Rakwalc R Agrawald G. K Shibatoc J Burowa M Puppalaf N 2008 Proteomics analysis of mature seed of four peanut cultivars using two-dimensional gel electrophoresis reveals distinct differential expression of storage, anti-nutritional, and allergenic proteins. ,175 3 321 329 - 103.
Kwaasi A. A Harfi H. A Parhar R. S Al-sedairy S. T Collison K. S Panzani R. C Al-mohanna F. A 1999 Allergy to Date fruits: characterization of antigens and allergens of fruits of the Date Palm (Phoenix dactylifera L.). 54 12 1270 1277 - 104.
Kwaasi A. A Harfi H. A Parhar R. S Collison K. S Al-sedairy S. T Al-mohanna F. A 2000 Cultivar-specific IgE-epitopes in Date ( L.) fruit allergy. Correlation of skin test reactivity and IgE-binding properties in selecting Date cultivars for allergen standardization. International Archives of Allergy and Immunology,123 2 137 144 - 105.
Kwaasi A. A Parhar R. S Tipirneni P Harfi H. A Al-sedairy S. T 1994 Cultivar-specific epitopes in date palm ( L.) pollenosis. Differential antigenic and allergenic properties of pollen from ten cultivars. International Archives of Allergy and Immunology,104 3 281 290 - 106.
La Mantia M., Lain, O., Caruso, T. & Testolin, R. (2005 SSR-based DNA fingerprints reveal the genetic diversity of Sicilian olive ( L.) germplasm. The journal of Horticultural Science & Biotechnology,80 8 628 632 - 107.
Di Felice, G. & Pini, C. (Lacovacci P Afferni C Butteroni C Pironi L Puggioni E. M Orlandi A Barletta B Tinghino R Ariano R Panzani R. C 2002 Comparison between the native glycosylated and the recombinant Cup a1 allergen: role of carbohydrates in the histamine release from basophils. ,32 11 1620 1627 - 108.
. ( .,Leduc ,V .,Charpin ,D .,Aparicio ,C . &Veber ,C Guerin ,L 2000 ). Allergy to cypress pollen: preparation of a reference and standardization extract in vivo. Allergie et Immunologie,32 3 101 103 . - 109.
Maeda M Kamamoto M Hino K Yamamoto S Kimura M Okano M Kimura Y 2005 Glycoform analysis of Japanese cedar pollen allergen, Cry j 1. Biotechnology, and Biochemistry,69 9 1700 1705 - 110.
Mari A Rasi C Palazzo P Scala E 2009 Allergen databases: current status and perspectives 9 376 383 - 111.
Maruyama-funstsuki W Fujino K Suzuki T Funatsuki H 2004 Quantification of a major allergenic protein in common buckwheat cultivars by an enzyme-linked immunosorbent assay (ELISA). ,21 39 44 - 112.
Matsumura D Nabe T Mizutani N Fujii M Kohno S 2006 Detection of new antigenic proteins in Japanese cedar pollen. 29 6 1162 1166 - 113.
Matthes A Schmitz-eiberger M 2009 Apple ( L. Borkh.) allergen Mal d 1: effect of cultivar, cultivation system, and storage conditions. Journal of Agricultural and Food Chemistry,57 22 10548 10553 - 114.
Mcneill J Barrie F. R Burdet H. M Demoulin V Hawksworth D. L Marhold K Nocolson D. H Prado J Silva P. C Skog J. E Wiersema J. H Turland N. J 2006 International Code of Botanical Nomenclature (Vienna Code) . - 115.
Midoro-horiuti T Goldblum R. M Kurosky A Goetz D. W Brooks E. G 1999 Isolation and characterization of the mountain Cedar (Juniperus ashei) pollen major allergen, Jun a 1. 104 3 Pt 1),608 612 - 116.
Midoro-horiuti T Schein C. H Mathura V Braun W Czerwinski E. W Togawa A Kondo Y Oka T Watanabe M Goldblum R. M 2006 Structural basis for epitope sharing between group 1 allergens of cedar pollen 43 6 509 518 - 117.
. ( .,Mistrello ,G .,Roncarolo ,D .,Zanoni ,D .,Zanotta ,S .,Amato ,S . &Falagiani ,P Ariano ,R 2002 ). Allergenic relevance of Cupressus arizonica pollen extract and biological characterization of the allergoid. International Archives of Allergy and Immunology,129 4 296 304 . - 118.
Mogensen J. E Wimmer R Larsen J. N Spangfort M. D Otzen D. E 2002 The major birch allergen, Bet v 1, shows affinity for a broad spectrum of physiological ligands. 277 26 23684 23692 - 119.
Mohapatra S. S Lockey R. F Polo F 2004 Weed pollenallergens. Allergens and Allergen Im munotherapy. Marcel Dekker, Inc. New York,207 222 - 120.
Morales S 2012 Desarrollo y aplicación de un sistema multiplex para la caracterización del polimorfismo de las proteínas alergénicas en el polen de distintas variedades de olivo ( L.). Doctoral thesis. Granada (Spain): University of Granada. - 121.
Morales S Castro A. J Jiménez-lópez J. C Florido F Rodríguez-garcía M. I Alché J. D 2012 A novel multiplex method for the simultaneous detection and relative quantification of pollen allergens. ,33 9-10 1367 1374 - 122.
Morales S Jiménez-lópez J. C Castro A. J Rodríguez-garcía M. I Alché J. D 2008 Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination Oxford,231 2 332 342 - 123.
Moreno-aguilar C 2008 Improving pollen immunotherapy: minor allergens and panallergens 36 1 26 - 124.
Mothes N Horak F Valenta R 2004 Transition from a botanical to a molecular classification in tree pollen allergy: implications for diagnosis and therapy 135 4 357 373 - 125.
Mothes N Westritschnig K Valenta R 2004 Tree pollen allergens. 18 165 184 - 126.
Moverare R Westritschnig K Svensson M Hayek B Bende M Pauli G Sorva R Haahtela T Valenta R Elfman L 2002 Different IgE Reactivity Profiles in Birch Pollen-Sensitive Patients from Six European Populations Revealed by Recombinant Allergens: An Imprint of Local Sensitization 128 4 325 335 - 127.
Medina Escobar, N., Ludemann, F., Botella, M.A., Valpuesta, V. & Schwab, W. (Munoz C Hoffmann T 2010 The strawberry fruit Fra a allergen functions in flavonoid biosynthesis. ,3 1 113 124 - 128.
Muzzalupo I Lombardo N Musacchio A Noce M. E Pellegrino G Perri E Sajjad A 2006 DNA sequence analysis of microsatellite markers enhances their efficiency for germplasm management in an Italian olive collection.131 352 359 - 129.
Nair A Adachi T 2002 Screening and selection of hypoallergenic buckwheat species. ,2 818 826 - 130.
Nakamura A Tanabe S Watanabe J Makino T 2005 Primary screening of relatively less allergenic wheat varieties. 51 13 204 206 - 131.
Namba M Kurose M Torigoe K Hino K Taniguchi Y Fukuda S Usui M Kurimoto M 1994 Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen. 353 124 128 - 132.
Di Donna, L., Moschidis, P. & Sindona, G. (Napoli A Aiello D 2008 Vegetable Proteomics: The Detection of Ole e 1 Isoallergens by Peptide Matching of MALDI MS/MS Spectra of Underivatized and Dansylated Glycopeptides 7 7 2723 2732 - 133.
Di Donna, L., Sajjad, A., Perri, E. & Sindona, G. (Napoli A Aiello D 2006 Profiling of hydrophilic proteins from Olea europaea olive pollen by MALDI TOF mass spectrometry. 78 10 3434 3443 - 134.
Niederberger V Laffer S Froschl R Kraft D Rumpold H Kapiotis S Valenta R Spitzauer S 1998 IgE antibodies to recombinant pollen allergens (Phl p 1, Phl p 2, Phl p 5, and Bet v 2) account for a high percentage of grass pollen-specific IgE. ,101 2 Pt 1),258 264 - 135.
a major allergen of Japanese cedar pollen, shows polymethylgalacturonase activity.Ohtsuki T Taniguchi Y Kohno K Fukuda S Usui M Kurimoto M 1995 Cry j 50 6 483 488 - 136.
Okano M Kimura Y Kino K Michigami Y Sakamoto S Sugata Y Maeda M Matsuda F Kimura M Ogawa T Nishizaki K 2004 Roles of major oligosaccharides on Cry j 1 in human immunoglobulin E and T cell responses ,34 5 770 778 - 137.
Okano M Kino K Takishita T Hattori H Ogawa T Yoshino T Yokoyama M Nishizaki K 2001 Roles of carbohydrates on Cry j 1, the major allergen of Japanese cedar pollen, in specific T-cell responses The108 1 101 108 - 138.
Di Giusto, F. (Ouazzani N Lumaret R Villemur P 1993 Leaf allozyme variation in cultivated and wild Olive trees (Olea europaea L.) 84 1 34 42 - 139.
Panzani R Yasueda H Shimizu T Shida T 1986 Cross-reactivity between the pollens of Cupressus sempervirens (common cypress) and of Cryptomeria japonica (Japanese Cedar). 57 1 26 30 - 140.
Pauli G Bessot J. C Dietemann-molard A Braun P. A Thierry R 1985 Celery sensitivity: clinical and immunological correlations with pollen allergy. 15 3 273 279 - 141.
Penon J. P 2000 Cypress arizona: allergic extracts with a diagnostic purpose.32 3 107 108 - 142.
Pharmacia Diagnostics AB ( Allergenic Plants. Systematics of common and rare allergens. Version 2.0 CD.2001 - 143.
Postigo I Guisantes J. A Negro J. M Rodriguez-pacheco R David-garcia D Martinez J 2009 Identification of 2 new allergens of Phoenix dactylifera using an immunoproteomics approach 19 6 504 507 - 144.
Radauer C Breiteneder H 2006 Pollen allergens are restricted to few protein families and show distinct patterns of species distribution ,117 1 141 147 - 145.
. ( .,Ribeiro ,H .,Cunha ,M . &Calado ,L Abreu ,I 2012 ). Pollen morphology and quality of twenty olive (Olea europaea L.) cultivars grown in Portugal. Acta Horticulturae (ISHS), Vol. 949, pp. 259- - 146.
Rur Mira. (2007 Localization of the main allergy protein in two apple cultivars grown in Sweden ,2007 3 pages. - 147.
Saito M Teranishi H 2002 Immunologic determination of the major allergen, Cry j 1, in Cryptomeria japonica pollen of 117 clones in Toyama prefecture: Some implications for further forestry research in pollinosis prevention 51 3 191 195 - 148.
Sakaguchi M Inouye S Taniai M Ando S Usui M Matuhasi T 1990 Identification of the second major allergen of Japanese Cedar pollen. 45 4 309 312 - 149.
Scheiner O 1993 Molecular and functional characterization of allergens: fundamental and practical aspects.105 22 653 658 - 150.
Schenk M. F Cordewener J. H. G America A. H. P Peters J Smulders M. J. M Gilissen L. J. W. J 2011 Proteomic analysis of the major birch allergen Bet v 1 predicts allergenicity for 15 birch species. ,74 8 1290 1300 - 151.
of PR-10 genes from eight Betula species and detection of BetSchenk M. F Cordewener J. H. G America A. H. P van’Peters J t Westende W. P. C Smulders M. J. M Gilissen L. J. W. J 2009 Characterization 1 isoforms in birch pollen. 9:24 - 152.
Schenk M. F Gilissen L. J. W. J Esselink G. D Smulders M. J. M 2006 Seven different genes encode a diverse mixture of isoforms of Bet v 1, the major birch pollen allergen 7 168 - 153.
Schenk M. F Thienpont C. N Koopman W. J. M Gilissen L. J. W. J Smulders M. J. M 2008 Phylogenetic relationships in Betula (Betulaceae) based on AFLP markers 4 4 911 924 - 154.
Seiberler S Scheiner O Kraft D Lonsdale D Valenta R 1994 Characterization of a birch pollen allergen, Bet v III, representing a novel class of Ca2+ binding proteins: specific expression in mature pollen and dependence of patient’s IgE binding on protein-bound Ca2+. ,13 15 3481 3486 - 155.
Shahali Y Majd A Pourpak Z Tajadod G Haftlang M Moin M 2007 Comparative study of the pollen protein contents in two major varieties of Cupressus arizonica planted in Tehran ,6 3 123 127 - 156.
Stahl Skov, P., Bindslev-Jensen, C. & Poulsen, L.K. (Skamstrup-hansen K Vieths S Vestergaard H 2001 Seasonal variation in food allergy to apple. 756 1-2 19 32 - 157.
Ladan Moghadam, A.R. (Soleimani A Alché J. D Castro A. J Rodríguez-garcía M. I 2012 Using Two-Dimensional Gel Electrophoresis approach for characterizing of the Ole e 1, an olive pollen major allergen ISHS),932 69 72 - 158.
Soleimani A Morales S Jiménez-lópez J. C Castro A. J Rodríguez-garcía M. I Alché J. D 2012 Differential expression and sequence polymorphism of the olive pollen allergen Ole e 1 in two Iranian cultivars. , in press. - 159.
Sone T Komiyama N Shimizu K Kusakabe T Morikubo K Kino K 1994 Cloning and sequencing of cDNA coding for Cry j I, a major allergen of Japanese cedar pollen 199 2 619 625 - 160.
Spangenberg G Petrovska N Kearney G. A Smith K. F 2006 Low-pollen-allergen ryegrasses: towards a continent free of hay fever? In: Allergy Matters: New approaches to allergy prevention and management Chapter 13,121 128 - 161.
Stäger J Wüthrich B Johansson S. G. O 1991 Spice allergy in celery-sensitive patients. 46 6 475 478 - 162.
Stewart G. A Mcwilliam A. S 2001 Endogenous function and biological significance of aeroallergens: an update. 1 1 95 103 - 163.
del Pozo, V., Fernandez-Gonzalez, D., Moreno-Grau, S., Moral, A., Lopez-Iglesias, C., Lahoz, C. & Seoane-Camba, J.A. (Suárez-cervera M Castells T Vega-maray A Civantos E 2008 Effects of air pollution on Cup a 3 allergen in Cupressus arizonica pollen grains. Asthma & Immunology,101 1 57 66 - 164.
Susani M Jertschin P Dolecek C Sperr W. R Valent P Ebner C Kraft D Valenta R Scheiner O 1995 High level expression of birch pollen profilin (Bet v 2) in Escherichia coli: purification and characterization of the recombinant allergen.215 1 250 263 - 165.
Swoboda I Scheiner O Kraft D Breitenbach M Heberle-bors E Vicente O 1994 A birch gene family encoding pollen allergens and pathogenesis-related proteins. 1219 2 457 464 - 166.
Takahashi Y Aoyama M 2006 Development of the simple method for measurement the content of Cry j 1 in the air by latex agglutination test.55 1 28 33 - 167.
Takhtajan A 1997 Diversity and classification of flowering plants , 643 pages. - 168.
Taneichi M Uehara M Katagiri M 1994 Analysis of birch pollen allergen. ,69 5 1154 1161 - 169.
Taniai M Ando S Usui M Kurimoto M Sakaguchi M Inouye S Matuhasi T 1988 N-terminal amino acid sequence of a major allergen of Japanese cedar pollen (Cry j 1).239 2 329 332 - 170.
a major allergen of Japanese cedar pollen, has pectate lyase enzyme activity. ,Taniguchi Y Ono A Sawatani M Nanba M Kohno K Usui M Kurimoto M Matuhasi T 1995 Cry j 50 1 90 93 - 171.
Di Felice, G., Focke, M., Westritschnig, K., Valenta, R. & Pini, C. (Tinghino R Twardosz A Barletta B Puggioni E. M Iacovacci P Butteroni C Afferni C Mari A Hayek B 2002 Molecular, structural, and immunologic relationships between different families of recombinant calcium-binding pollen allergens The109 2 Pt 1),314 320 - 172.
Togawa A Panzani R. C Garza M. A Kishikawa R Goldblum R. M Midoro-horiuti T 2006 Identification of italian cypress (Cupressus sempervirens) pollen allergen Cup s 3 using homology and cross-reactivity. Asthma & Immunology,97 3 336 342 - 173.
Trujillo I Rallo L 1995 Identifying olive cultivars by isozyme analysis.120 318 324 - 174.
Twardosz A Hayek B Seiberler S Vangelista L Elfman L Grönlund H Kraft D Valenta R 1997 Molecular characterization, expression in Escherichia coli, and epitope analysis of a two EF-hand calcium-binding birch pollen allergen, Bet v 4. ,239 1 197 204 - 175.
Valenta R Breiteneder H Petternburger K Breitenbach M Rumpold H Kraft D Scheiner O 1991a Homology of the major birch-pollen allergen, Bet v I, with the major pollen allergens of alder, hazel, and hornbeam at the nucleic acid level as determined by cross-hybridization. 87 3 677 682 - 176.
Valenta R Duchene M Breitenbach M Pettenburger K Koller L Rumpold H Scheiner O Kraft D 1991b A low molecular weight allergen of white birch () is highly homologous to human profilin. International Archives of Allergy and Applied Immunology,94 1-4 368 370 - 177.
Valenta R Duchene M Pettenburger K Sillaber C Valent P Bettelheim P Breitenbach M Rumpold H Kraft D Scheiner O 1991c Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitised individuals. ,253 5019 557 560 - 178.
Valenta R Ferreira F Grote M Swoboda I Vrtala S Duchene M Deviller P Meagher R. B Mckinney E Heberle-bors E Krafts D Scheiners O 1993 Identification of profilin as an actin-binding protein in higher plants. ,268 30 22777 22781 - 179.
Van Ree R 2002 Isoallergens: a clinically relevant phenomenon or just a product of cloning?. .32 975 978 - 180.
Vieths S Frank E Scheurer S Meyer H. E Hrazdina G Haustein D 1998 Characterization of a new IgE-binding 35-kDa protein from birch pollen with cross-reacting homologues in various plant foods. ,47 3 263 272 - 181.
Schöning, B. & Aulepp, H. (Vieths S Jankiewicz A 1994 Apple allergy: the IgE-binding potency of apple strains is related to the occurence of the 18-kDa allergen. Allergy,49 4 262 271 - 182.
Vieths S Scheurer S Ballmer-weber B 2002 Current understanding of cross-reactivity of food allergens and pollen. ,964 47 68 - 183.
van de Weg, W.E., van der Heide, S., Kerkhof, M., Arens, P., Heijerman-Peppelman, G. & Dubois, A. E. (Vlieg-boerstra B. J 2011 Identification of low allergenic apple cultivars using skin prick tests and oral food challenges. ,66 4 491 498 - 184.
Schmid Grendelmeier, P., Cromwell, O. & Wüthrich, B. (Wahl R 1996 investigation of cross-reactivity between birch and ash pollen allergen extracts. The Journal of Allergy and Clinical Immunology,98 1 99 106 - 185.
Waisel Y Geller-bernstein C 1996 Reliability of olive pollen extracts for skin prick tests. ,98 3 715 716 - 186.
Wallner M Erler A Hauser M Klinglmayr E Gardemaier G Vogel L Mari A Bohle B Briza P Ferreira F 2009a Immunologic characterization of isoforms of Car b 1 and Que a 1, the major hornbeam and oak pollen allergens. ,64 3 452 460 - 187.
Wallner M Himly M Neubauer A Erler A Hauser M Asam C Mutschlechner S Ebner C Briza P Ferreira F 2009b The influence of recombinant production on the immunologic behavior of birch pollen isoallergens. ,4 12 e8457. - 188.
Wiebicke K Schlenvoigt G Jäger L 1987 Allergologic-immunochemical study of various tree pollens. I. Characterization of antigen and allergen components in birch, beech, alder, hazel and oak pollens. ,33 3 181 190 - 189.
Wiedemann P Giehl K Almo S. C Fedorov A. A Girvin M Steinberger P Rudiger M Ortner M Sippl M Dolecek C Kraft D Jockusch B Valenta R 1996 Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody271 47 29915 29921 - 190.
Wüthrich B Dietschi R 1985 The celery-carrot-mugwort-condiment syndrome: skin test and RAST results. ,115 11 258 264 - 191.
Yasueda H Yui Y Shimizu T Shida T 1983 Isolation and partial characterization of the major allergen from Japanese cedar () pollen. The Journal of Allergy and Clinical Immunology,71 1 Pt 1),77 86 - 192.
Yman L 1982 Botanical relations and immunological cross-reactions in pollen allergy. . Uppsala. Sweden. - 193.
Yman L 2001 Allergenic Plants. Systematics of common and rare allergens. Uppsala, Sweden. - 194.
Zafra A 2007 Caracterización preliminar del polimorfismo de la proteína alergénica Ole e 5 en el polen del olivo de distintos cultivares. Master thesis. Granada (Spain): University of Granada.