Released this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
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We wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
IntechOpen is proud to announce that 191 of our authors have made the Clarivate™ Highly Cited Researchers List for 2020, ranking them among the top 1% most-cited.
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Throughout the years, the list has named a total of 261 IntechOpen authors as Highly Cited. Of those researchers, 69 have been featured on the list multiple times.
\n\n\n\n
Released this past November, the list is based on data collected from the Web of Science and highlights some of the world’s most influential scientific minds by naming the researchers whose publications over the previous decade have included a high number of Highly Cited Papers placing them among the top 1% most-cited.
\n\n
We wish to congratulate all of the researchers named and especially our authors on this amazing accomplishment! We are happy and proud to share in their success!
Note: Edited in March 2021
\n'}],latestNews:[{slug:"intechopen-supports-asapbio-s-new-initiative-publish-your-reviews-20220729",title:"IntechOpen Supports ASAPbio’s New Initiative Publish Your Reviews"},{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"}]},book:{item:{type:"book",id:"10544",leadTitle:null,fullTitle:"Antioxidants - Benefits, Sources, Mechanisms of Action",title:"Antioxidants",subtitle:"Benefits, Sources, Mechanisms of Action",reviewType:"peer-reviewed",abstract:"Antioxidants are one of the most sought-after biological compounds of interest to both scientific and nonscientific communities. The term gained popularity with the advent of identifying these compounds as having the ability to maintain health and wellness by combating against pathways leading to non-communicable diseases. This book covers several aspects of antioxidants—mechanisms of action, assays of measuring potency, sources, and even methods of isolation and identification. While it may seem these aspects have been covered in depth in several publications before this, this book intends to be positioned as an update, especially since the area of antioxidant research is as dynamic as ever. There are several chapters that might be of interest to health buffs, specifically those who are quite keen on maintaining health and wellness.",isbn:"978-1-83968-865-2",printIsbn:"978-1-83968-864-5",pdfIsbn:"978-1-83968-866-9",doi:"10.5772/intechopen.92918",price:159,priceEur:175,priceUsd:205,slug:"antioxidants-benefits-sources-mechanisms-of-action",numberOfPages:646,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"fe6b71d10cd19383975798a81e63e57b",bookSignature:"Viduranga Waisundara",publishedDate:"September 8th 2021",coverURL:"https://cdn.intechopen.com/books/images_new/10544.jpg",numberOfDownloads:10342,numberOfWosCitations:7,numberOfCrossrefCitations:29,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:49,numberOfDimensionsCitationsByBook:1,hasAltmetrics:1,numberOfTotalCitations:85,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"September 17th 2020",dateEndSecondStepPublish:"October 15th 2020",dateEndThirdStepPublish:"December 14th 2020",dateEndFourthStepPublish:"March 4th 2021",dateEndFifthStepPublish:"May 3rd 2021",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"194281",title:"Dr.",name:"Viduranga Y.",middleName:null,surname:"Waisundara",slug:"viduranga-y.-waisundara",fullName:"Viduranga Y. Waisundara",profilePictureURL:"https://mts.intechopen.com/storage/users/194281/images/system/194281.jpg",biography:"Dr. Viduranga Waisundara obtained her Ph.D. in Food Science\nand Technology from the Department of Chemistry, National\nUniversity of Singapore, in 2010. She was a lecturer at Temasek Polytechnic, Singapore from July 2009 to March 2013.\nShe relocated to her motherland of Sri Lanka and spearheaded the Functional Food Product Development Project at the\nNational Institute of Fundamental Studies from April 2013 to\nOctober 2016. She was a senior lecturer on a temporary basis at the Department of\nFood Technology, Faculty of Technology, Rajarata University of Sri Lanka. She is\ncurrently Deputy Principal of the Australian College of Business and Technology –\nKandy Campus, Sri Lanka. She is also the Global Harmonization Initiative (GHI)",institutionString:"Australian College of Business & Technology",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"13",totalChapterViews:"0",totalEditedBooks:"11",institution:{name:"Kobe College",institutionURL:null,country:{name:"Japan"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"43",title:"Biochemistry",slug:"biochemistry-genetics-and-molecular-biology-biochemistry"}],chapters:[{id:"77150",title:"Antioxidants: Pharmacothearapeutic Boon for Diabetes",doi:"10.5772/intechopen.98587",slug:"antioxidants-pharmacothearapeutic-boon-for-diabetes",totalDownloads:228,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Glucose-induced oxidative stress can be found related to “glucose variability” and “glucose memory”. The irregular low and elevated glucose conditions cause damage to endothelial cell function than a steady, constant rise in level of glucose. Activation of PKC, NADPH oxidases, and mitochondrial oxidants are some of the pathways exhibited as a result of this aggravated cellular response. Regarding glucose memory, long after the normalization elevated level of glucose in the endothelial cells of diabetic rats and culture, a existance or ‘memory’ of induced basement membrane mRNA is expressed. This demonstrates that glucose causes dangerous long-term effects beyond the hyperglycemia period. Oxidative stress give rise to glucotoxicity and lipotoxicity which are phenomena’s related to diabetes. Following the pathogenesis of diabetes, hyperglycemia and hyperlipidemia exerts a supplementary toxic effect on the beta-cells. So, hyperglycemia can be considered as a requirement for the destructive effects of lipotoxicity. Thus glucolipotoxicity can be considered as a substitute for lipotoxicity which relates the detrimental correlation between lipids and beta-cell function. Generally, the antioxidant pharmacotherapy can be coupled with drugs to boost the natural cellular defense mechanisms as the naturally existing antioxidant components, which neutralizes free radical damage. This considers antioxidant a boon tool for pharmacotherapeutic agent.",signatures:"Varuna Suresh, Amala Reddy, Pavithra Muthukumar and Thendarl Selvam",downloadPdfUrl:"/chapter/pdf-download/77150",previewPdfUrl:"/chapter/pdf-preview/77150",authors:[{id:"335347",title:"Assistant Prof.",name:"Amala",surname:"Reddy",slug:"amala-reddy",fullName:"Amala Reddy"},{id:"335350",title:"Ms.",name:"Thendarl",surname:"Selvam",slug:"thendarl-selvam",fullName:"Thendarl Selvam"},{id:"335352",title:"Ms.",name:"Pavithra",surname:"Muthukumar",slug:"pavithra-muthukumar",fullName:"Pavithra Muthukumar"},{id:"355815",title:"Ms.",name:"Varuna",surname:"Suresh",slug:"varuna-suresh",fullName:"Varuna Suresh"}],corrections:null},{id:"75042",title:"Micronutrient Antioxidants in the Chemoprevention of Breast Cancer and Effect on Breast Cancer Outcomes",doi:"10.5772/intechopen.95886",slug:"micronutrient-antioxidants-in-the-chemoprevention-of-breast-cancer-and-effect-on-breast-cancer-outco",totalDownloads:301,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Breast cancer remains one of the most frequent cancers affecting women globally. The incidence of breast cancer is rising due to improved screening and awareness, and there is epidemiological data signifying an interaction among environmental and biological risk factors in the development and progress of breast cancer. There is substantial experimental data of the protective effect of micronutrient antioxidants for breast cancer via alteration of many signaling pathways and molecular events including inducing apoptosis, and inhibition of breast cancer cell proliferation and invasion. The main focus of this review is to examine past and current epidemiological evidence that suggests that nutritional micronutrients with antioxidant properties in dietary or supplemental form may be beneficial in protecting women against breast cancer and affect outcomes.",signatures:"Donovan McGrowder, Fabian Miller, Chukwuemeka Nwokocha, Cameil Wilson-Clarke, Melisa Anderson, Lennox Anderson-Jackson and Lowen Williams",downloadPdfUrl:"/chapter/pdf-download/75042",previewPdfUrl:"/chapter/pdf-preview/75042",authors:[{id:"155804",title:"Dr.",name:"Donovan",surname:"McGrowder",slug:"donovan-mcgrowder",fullName:"Donovan McGrowder"},{id:"158092",title:"Mr.",name:"Lennox",surname:"Anderson-Jackson",slug:"lennox-anderson-jackson",fullName:"Lennox Anderson-Jackson"},{id:"247363",title:"Dr.",name:"Chukwuemeka",surname:"Nwokocha",slug:"chukwuemeka-nwokocha",fullName:"Chukwuemeka Nwokocha"},{id:"280662",title:"Ms.",name:"Melisa",surname:"Anderson",slug:"melisa-anderson",fullName:"Melisa Anderson"},{id:"280664",title:"Mr.",name:"Fabian",surname:"Miller",slug:"fabian-miller",fullName:"Fabian Miller"},{id:"345469",title:"Dr.",name:"Cameil",surname:"Wilson-Clarke",slug:"cameil-wilson-clarke",fullName:"Cameil Wilson-Clarke"},{id:"345470",title:"Mr.",name:"Lowen",surname:"Williams",slug:"lowen-williams",fullName:"Lowen Williams"}],corrections:null},{id:"75026",title:"Role of Antioxidants Supplementation in the Treatment of Male Infertility",doi:"10.5772/intechopen.95891",slug:"role-of-antioxidants-supplementation-in-the-treatment-of-male-infertility",totalDownloads:441,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Nutritional utilization of antioxidants, such as vitamins C, E, ß-Carotene and micronutrients, such as folate and zinc, have been shown to be critically essential for normal semen quality and reproductive function. However, it is still, a large knowledge gap exists concerning the role of antioxidants on semen parameters and the role in treatment of male subfertility. Therefore, the current review article designed to find out the positive effect of antioxidants on semen quality, alterations in physiological functions of spermatozoa and infertility treatment It is advisable that patients with oxidative DNA disruption should be asked to take a simple course of antioxidants prior to undertaking assisted reproduction treatment (ART). In conclusion, antioxidant may be employed as a potent antioxidant and may improve infertility treatment outcomes with ART.",signatures:"Houda Amor, Nyaz Shelko, Massooma Mohammed, Peter Michael Jankowski and Mohamad Eid Hammadeh",downloadPdfUrl:"/chapter/pdf-download/75026",previewPdfUrl:"/chapter/pdf-preview/75026",authors:[{id:"92657",title:"Prof.",name:"Mohamad Eid",surname:"Hammadeh",slug:"mohamad-eid-hammadeh",fullName:"Mohamad Eid Hammadeh"},{id:"337019",title:"Dr.",name:"Houda",surname:"Amor",slug:"houda-amor",fullName:"Houda Amor"},{id:"337221",title:"Dr.",name:"Nyaz",surname:"Shelko",slug:"nyaz-shelko",fullName:"Nyaz Shelko"},{id:"345467",title:"Dr.",name:"Massooma",surname:"Mohsammed",slug:"massooma-mohsammed",fullName:"Massooma Mohsammed"},{id:"345468",title:"Dr.",name:"Peter Michael",surname:"Jankowski",slug:"peter-michael-jankowski",fullName:"Peter Michael Jankowski"}],corrections:null},{id:"74927",title:"Antioxidants in Female Reproductive Biology",doi:"10.5772/intechopen.95937",slug:"antioxidants-in-female-reproductive-biology",totalDownloads:326,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:0,abstract:"Human female reproductive biology is a complex system and its pathologies are varied. However, majority of the pathologic processes involves the role of reactive oxygen species (ROS). Imbalance between the ROS and antioxidants results in oxidative stress (OS). OS is the pathognomonic factor in various female reproductive system ailments. OS contributes to the pathophysiology of infertility, pregnancy related complications, endometriosis, ovarian cancers, etc. Evidence of elevated oxidative stress biomarkers can be found in various inflammatory conditions. Numerous strategies have been postulated for management of OS related pathologic conditions. Antioxidants supplementation may play a crucial in prevention and management of these conditions. However, robust evidence is needed to support the role of antioxidants supplementation in various female reproductive disorders.",signatures:"Banashree Nath and Hirok Roy",downloadPdfUrl:"/chapter/pdf-download/74927",previewPdfUrl:"/chapter/pdf-preview/74927",authors:[{id:"335983",title:"Assistant Prof.",name:"Banashree",surname:"Nath",slug:"banashree-nath",fullName:"Banashree Nath"},{id:"335985",title:"Dr.",name:"Hirok",surname:"Roy",slug:"hirok-roy",fullName:"Hirok Roy"}],corrections:null},{id:"74380",title:"Thiol Reduction and Cardiolipin Improve Complex I Activity and Free Radical Production in Liver Mitochondria of Streptozotocin-Induced Diabetic Rats",doi:"10.5772/intechopen.95112",slug:"thiol-reduction-and-cardiolipin-improve-complex-i-activity-and-free-radical-production-in-liver-mito",totalDownloads:280,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Mitochondrial reactive oxygen species (ROS) are involved in diabetic liver disease development. Diabetes impairs complex I activity and increases ROS production in liver mitochondria. The complex I produces ROS in forward electron transfer (FET) or in reverse electron transfer (RET) modes depending on the site of electron transfer blocking and the availability of respiratory substrates. Complex I activity depends on the phospholipid cardiolipin and the redox state of reactive thiols in the enzyme. Neither the underlying factors leading to complex I dysfunction nor the mode of ROS production have been elucidated in liver mitochondria in diabetes. We tested in liver mitochondria from streptozotocin (STZ) -induced diabetic rats if the addition of cardiolipin or β-mercaptoethanol, a thiol reducing agent, recovers complex I activity and decreases ROS production with substrates inducing ROS production in FET or RET modes. Decreased complex I activity and enhanced ROS generation in FET mode was detected in mitochondria from diabetic rats. Complex I activity was fully restored with the combined treatment with cardiolipin plus β-mercaptoethanol, which also abated ROS generation in FET mode. This suggest that therapies restoring cardiolipin and reducing mitochondrial thiols might be useful to counteract impaired complex I activity and excessive ROS production in liver mitochondria in diabetes.",signatures:"Manjury Jatziry Hernández-Esparza, Claudia Guadalupe Flores-Ledesma, Rocío Montoya-Pérez, Elizabeth Calderón-Cortés, Alfredo Saavedra-Molina, Alain Raimundo Rodríguez-Orozco and Christian Cortés-Rojo",downloadPdfUrl:"/chapter/pdf-download/74380",previewPdfUrl:"/chapter/pdf-preview/74380",authors:[{id:"198879",title:"BSc.",name:"Claudia Guadalupe",surname:"Flores-Ledesma",slug:"claudia-guadalupe-flores-ledesma",fullName:"Claudia Guadalupe Flores-Ledesma"},{id:"198880",title:"Ph.D. Student",name:"Manjury Jatziry",surname:"Hernández-Esparza",slug:"manjury-jatziry-hernandez-esparza",fullName:"Manjury Jatziry Hernández-Esparza"},{id:"198882",title:"Dr.",name:"Alain Raimundo",surname:"Rodriguez-Orozco",slug:"alain-raimundo-rodriguez-orozco",fullName:"Alain Raimundo Rodriguez-Orozco"},{id:"254069",title:"Dr.",name:"Alfredo",surname:"Saavedra-Molina",slug:"alfredo-saavedra-molina",fullName:"Alfredo Saavedra-Molina"},{id:"335120",title:"Prof.",name:"Christian",surname:"Cortes-Rojo",slug:"christian-cortes-rojo",fullName:"Christian Cortes-Rojo"},{id:"335449",title:"Dr.",name:"Rocío",surname:"Montoya - Pérez",slug:"rocio-montoya-perez",fullName:"Rocío Montoya - Pérez"},{id:"335594",title:"Dr.",name:"Elizabeth",surname:"Calderon-Cortes",slug:"elizabeth-calderon-cortes",fullName:"Elizabeth Calderon-Cortes"}],corrections:null},{id:"75568",title:"Antioxidant and Oxidative Stress",doi:"10.5772/intechopen.96643",slug:"antioxidant-and-oxidative-stress",totalDownloads:309,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Antioxidants are compounds that eliminate oxidative stress in biological systems. Oxidative stress is caused by various radicals formed in the system as a result of oxygen entering the biological system. Structures with unpaired electron are either free radicals or radical ions. Antioxidants neutralize free radicals or radical ions due to the unpaired electron in their structure. The radical ions formed as a result of oxidation is removed from the system without damaging the biological system with the effect of antioxidants. There are many free radicals and radical ions. Among these radical groups are radical ions formed by oxygen which are important for biological systems. Antioxidants are responsible for the destruction of such radicals.",signatures:"Betül Çalişkan and Ali Cengiz Çalişkan",downloadPdfUrl:"/chapter/pdf-download/75568",previewPdfUrl:"/chapter/pdf-preview/75568",authors:[{id:"199110",title:"Dr.",name:"Betül",surname:"Çalişkan",slug:"betul-caliskan",fullName:"Betül Çalişkan"},{id:"208732",title:"Dr.",name:"Ali Cengiz",surname:"Çalişkan",slug:"ali-cengiz-caliskan",fullName:"Ali Cengiz Çalişkan"}],corrections:null},{id:"75228",title:"Natural Antioxidants to the Rescue?",doi:"10.5772/intechopen.96132",slug:"natural-antioxidants-to-the-rescue-",totalDownloads:290,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Natural antioxidant compounds have different mechanisms of treatment and prevention against various diseases due to their richest ingredients. There are several antioxidants used today, such as phytogenic ingredients, flavonoids, capsaicin, spirulina, beta-glucan, polyphenol etc. Besides the outbreak of diseases, the ability to scavenge oxidative conditions of the natural antioxidants have been notably important. Thereby, therapeutic strategies of diseases have been interested by researchers. Try to seek a kind of effects of natural antioxidants to various diseases, especially viral or pandemic diseases are being important nowadays. This chapter we’ll mention about how to viral or pandemic disease’s effects on oxidative status in both animals and humans, and what kind of phytochemical ingredients would be a positive effect on. At the same time, the latest advances about these natural antioxidant compounds and pharmaceuticals will be critically highlighted and discussed with newest literatures.",signatures:"Cenk Aydin and Nilay Seyidoglu",downloadPdfUrl:"/chapter/pdf-download/75228",previewPdfUrl:"/chapter/pdf-preview/75228",authors:[{id:"191307",title:"Dr.",name:"Nilay",surname:"Seyidoglu",slug:"nilay-seyidoglu",fullName:"Nilay Seyidoglu"},{id:"192225",title:"Prof.",name:"Cenk",surname:"Aydin",slug:"cenk-aydin",fullName:"Cenk Aydin"}],corrections:null},{id:"74859",title:"An Antioxidant Defense System in Radiation-Resistant Bacterium Deinococcus geothermalis against Oxidative Stress",doi:"10.5772/intechopen.95658",slug:"an-antioxidant-defense-system-in-radiation-resistant-bacterium-em-deinococcus-geothermalis-em-agains",totalDownloads:220,totalCrossrefCites:1,totalDimensionsCites:2,hasAltmetrics:0,abstract:"A radiation-resistant bacterium, Deinococcus geothermalis has various stress response mechanisms, including antioxidation. Features that maintain vitality at high radiation doses include the following: enzymatic scavengers of ROS such as catalase, SOD, and peroxidase; strain-specific DNA repair systems such as Deinococcal unique proteins; non-enzymatic responses such as manganese complexes, carotenoids, and DNA-binding proteins. This chapter summarizes the primary response mechanism by redox balance centered on the cystine transporter. It also reviews action characteristics of DNA-binding protein Dps and a putative LysR family protein, and effects on loss of function of the carotenoid biosynthesis genes by transposition of insertion sequences. Environmental adaptation and molecular evolution of radiation-resistant bacterium are also considered to explain the potentials of molecular behavior induced by oxidative stress.",signatures:"Chanjae Lee, Min K. Bae and Sung-Jae Lee",downloadPdfUrl:"/chapter/pdf-download/74859",previewPdfUrl:"/chapter/pdf-preview/74859",authors:[{id:"334388",title:"Prof.",name:"Sung-Jae",surname:"Lee",slug:"sung-jae-lee",fullName:"Sung-Jae Lee"},{id:"334394",title:"MSc.",name:"Chanjae",surname:"Lee",slug:"chanjae-lee",fullName:"Chanjae Lee"},{id:"334395",title:"MSc.",name:"Min K.",surname:"Bae",slug:"min-k.-bae",fullName:"Min K. Bae"}],corrections:null},{id:"74332",title:"The Two Sides of Dietary Antioxidants in Cancer Therapy",doi:"10.5772/intechopen.94988",slug:"the-two-sides-of-dietary-antioxidants-in-cancer-therapy",totalDownloads:570,totalCrossrefCites:3,totalDimensionsCites:4,hasAltmetrics:0,abstract:"Cancer is a major cause of mortality around the world, representing about 13% of deaths on the planet. Among the available cancer treatments, chemotherapy is most frequently utilized compared to other treatments such as surgery and radiotherapy. Many dietary antioxidants have proven to effectively prevent oxidative stress, which has been noted in many disease pathogeneses, including cancer. However, during chemotherapy or radiotherapy treatment of cancer patients, antioxidants are used as an adjuvant treatment. The use of a proof-based technique is advised in determining the supplements most suited to cancer patients. Though there are numerous opinions about the dangers and advantages of antioxidants, it is reasonable to conclude that side effects caused by antioxidants, for now, remain unclear for patients during cancer treatment, aside from smokers during radiotherapy. In this report, details of the effectiveness of antioxidants on cancer treatment aiding in the reduction of cancer therapy side effects are discussed.",signatures:"Musbau Adewumi Akanji, Heritage Demilade Fatinukun, Damilare Emmanuel Rotimi, Boluwatife Lawrence Afolabi and Oluyomi Stephen Adeyemi",downloadPdfUrl:"/chapter/pdf-download/74332",previewPdfUrl:"/chapter/pdf-preview/74332",authors:[{id:"65667",title:"Dr.",name:"Oluyomi",surname:"Adeyemi",slug:"oluyomi-adeyemi",fullName:"Oluyomi Adeyemi"},{id:"123899",title:"Prof.",name:"Musbau",surname:"Akanji",slug:"musbau-akanji",fullName:"Musbau Akanji"},{id:"307142",title:"Mr.",name:"Damilare",surname:"Rotimi",slug:"damilare-rotimi",fullName:"Damilare Rotimi"},{id:"334269",title:"Mr.",name:"Lawrence Boluwatife",surname:"Afolabi",slug:"lawrence-boluwatife-afolabi",fullName:"Lawrence Boluwatife Afolabi"},{id:"334270",title:"Mr.",name:"Heritage Demilade",surname:"Fatinikun",slug:"heritage-demilade-fatinikun",fullName:"Heritage Demilade Fatinikun"}],corrections:null},{id:"74999",title:"Antioxidant and Infertility",doi:"10.5772/intechopen.95791",slug:"antioxidant-and-infertility",totalDownloads:143,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Unexplained sub-fertility is commonly identified if couples fail to conceive after 1 yr. of everyday unprotected sexual intercourse even though investigations for ovulation, tubal patency and semen evaluation are ordinary. Many previous studies had shown that oxidative stress plays an important role in human fertility. Free radicals are neutralized by an elaborate antioxidant defense system. In a healthy body, pro-oxidants and antioxidants maintain a ratio and a shift in this ratio towards pro-oxidants gives rise to oxidative stress. There are two types of antioxidants in the human body: enzymatic and non-enzymatic antioxidants. Under normal conditions, antioxidants convert ROS to H2O to prevent overproduction of ROS. All cells in the human body are capable of synthesizing glutathione specially the liver. Free radicals appear to have a physiological role in female reproductive system in many different processes such as: oocyte maturation, fertilization, luteal regression, endometrial shedding and progesterone production by the corpus luteum. Protection from ROS is afforded by scavengers present in both male and female reproductive tract fluids, as well as in seminal plasma elevated concentrations of ROS in these environments may have detrimental effects on the spermatozoa, oocytes, sperm oocyte interaction and embryos both in the Fallopian tube and the peritoneal cavity; therefore oxidative stress modulates a host of reproductive pathologies affecting natural fertility in a woman’s life.",signatures:"Huda Mahmood Shakir",downloadPdfUrl:"/chapter/pdf-download/74999",previewPdfUrl:"/chapter/pdf-preview/74999",authors:[{id:"332658",title:"Associate Prof.",name:"Huda Mahmood",surname:"Alfatal",slug:"huda-mahmood-alfatal",fullName:"Huda Mahmood Alfatal"}],corrections:null},{id:"74706",title:"The Role of Lycopene in Chronic Lung Diseases",doi:"10.5772/intechopen.95468",slug:"the-role-of-lycopene-in-chronic-lung-diseases",totalDownloads:399,totalCrossrefCites:1,totalDimensionsCites:3,hasAltmetrics:1,abstract:"Lycopene, a naturally occurring non-provitamin A carotenoid pigment, is responsible for the red to pink colors in tomato, watermelon, red bell peppers, and pink guava. There are many health benefits attributed to lycopene including but not limited to its antioxidant activity. According to the American Lung Association’s State of Lung Cancer, lung cancer is still the leading cause of cancer death in the United States. Other chronic lung diseases such as asthma, emphysema, and chronic obstructive pulmonary disease are high prevalence. This chapter summarizes lycopene’s protective role against lung diseases in both in vitro and in vivo studies. While it has been demonstrated that circulating lycopene can be used as a biomarker for several lung diseases, further studies are warranted to establish that. We aim to provide insights into how lycopene can remedy for lung diseases, including lung cancer.",signatures:"Emilio Balbuena, Junrui Cheng and Abdulkerim Eroglu",downloadPdfUrl:"/chapter/pdf-download/74706",previewPdfUrl:"/chapter/pdf-preview/74706",authors:[{id:"336012",title:"Prof.",name:"Abdulkerim",surname:"Eroglu",slug:"abdulkerim-eroglu",fullName:"Abdulkerim Eroglu"},{id:"343981",title:"B.Sc.",name:"Emilio",surname:"Balbuena",slug:"emilio-balbuena",fullName:"Emilio Balbuena"},{id:"343982",title:"Dr.",name:"Junrui",surname:"Cheng",slug:"junrui-cheng",fullName:"Junrui Cheng"}],corrections:null},{id:"75198",title:"Management of Diabetic Eye Disease Using Carotenoids and Nutrients",doi:"10.5772/intechopen.96064",slug:"management-of-diabetic-eye-disease-using-carotenoids-and-nutrients",totalDownloads:308,totalCrossrefCites:0,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Diabetic retinopathy is the leading cause of blindness and visual disability globally among working-age adults. Until recently, diabetic eye disease is primarily regarded by its microvasculature complications largely characterized by progressive retinopathy and macular edema. However, a growing body of evidence suggests that hyperglycemia-induced oxidative stress and inflammation play an integral role in the early pathogenesis of diabetic retinopathy by potentiating retinal neurodegeneration. The onset of type 2 diabetes mellitus starts with insulin resistance leading to insulin deficiency, hyperglycemia, and dyslipidemia. Which in turn enhances the pro-oxidant and pro-inflammatory pathways. Additionally, various poor dietary behaviors along with obesity worsen physiological state in diabetics. However, decreased levels and depletion of the endogenous antioxidant defense system in the retina can be sufficiently augmented via carotenoid vitamin therapy. Therefore, dietary supplementation of antioxidant micronutrients particularly macular carotenoids lutein, zeaxanthin and meso-zeaxanthin that promote retinal health and optimal visual performance, may serve as an adjunctive therapy in the management of diabetic eye disease.",signatures:"Drake W. Lem, Dennis L. Gierhart and Pinakin Gunvant Davey",downloadPdfUrl:"/chapter/pdf-download/75198",previewPdfUrl:"/chapter/pdf-preview/75198",authors:[{id:"48794",title:"Prof.",name:"Pinakin Gunvant",surname:"Davey",slug:"pinakin-gunvant-davey",fullName:"Pinakin Gunvant Davey"},{id:"339744",title:"Mr.",name:"Drake W.",surname:"Lem",slug:"drake-w.-lem",fullName:"Drake W. Lem"},{id:"346029",title:"Dr.",name:"Dennis L.",surname:"Gierhart",slug:"dennis-l.-gierhart",fullName:"Dennis L. Gierhart"}],corrections:null},{id:"74790",title:"Antioxidant Activity: The Presence and Impact of Hydroxyl Groups in Small Molecules of Natural and Synthetic Origin",doi:"10.5772/intechopen.95616",slug:"antioxidant-activity-the-presence-and-impact-of-hydroxyl-groups-in-small-molecules-of-natural-and-sy",totalDownloads:576,totalCrossrefCites:3,totalDimensionsCites:5,hasAltmetrics:0,abstract:"Polyhydroxylated natural phenolic compounds, especially those with low molecular weights, are characterized by their ability to eliminate free radicals as they act as strong antioxidants. The various types of phenolic compounds represent the most important natural antioxidants in addition to some vitamins. The chemical structures of these compounds is discussed in details with their action mechanisms to remove free radicals and prevent many incurable and malignant diseases. In addition to these natural compounds, the last two decades have witnessed increased attempts by many scientific groups and research centers to synthesize chemical compounds in large quantities to mimic these natural compounds, but at a lower cost and greater biological effectiveness. Herein, we conduct a chemical survey of relevant synthetic compounds containing the hydroxyl groups prepared in chemical laboratories and studied for their biological efficacies, such as their effectiveness as antioxidants, as well as the mechanism of elimination of free radicals.",signatures:"Mohammed Ali Al-Mamary and Ziad Moussa",downloadPdfUrl:"/chapter/pdf-download/74790",previewPdfUrl:"/chapter/pdf-preview/74790",authors:[{id:"300774",title:"Dr.",name:"Ziad",surname:"Moussa",slug:"ziad-moussa",fullName:"Ziad Moussa"},{id:"335205",title:"Dr.",name:"Mohammed Ali",surname:"Al-Mamary",slug:"mohammed-ali-al-mamary",fullName:"Mohammed Ali Al-Mamary"}],corrections:null},{id:"74807",title:"Vitamin C and Sepsis",doi:"10.5772/intechopen.95623",slug:"vitamin-c-and-sepsis",totalDownloads:391,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Vitamin C is a supplement used orally by several people globally. It may help in many other conditions, like sepsis, which is caused by an infection that leads to an imbalanced immune response involving pro (e.g., TNF-α, IL-1, IL-2, IL-6) and anti-inflammatory (e.g., IL-10, IL-4, IL-7) cytokines. Ascorbic acid is an antioxidant and acts against reactive oxygen species. At the same time, this vitamin influences cellular immune signaling, avoiding exacerbated transcription of pro-inflammatory cytokines. Very high intravenous doses have already shown to be beneficial in septic patients. Some clinical trials are still running to evaluate the real impact of vitamin C in this condition. To the moment, the combination of low-dose corticosteroids, high-dose parenteral ascorbate, and thiamine seems to be the most effective supportive treatment that could help septic patients recover.",signatures:"Adriana Françozo de Melo, Giulia Oliveira Timo and Mauricio Homem-de-Mello",downloadPdfUrl:"/chapter/pdf-download/74807",previewPdfUrl:"/chapter/pdf-preview/74807",authors:[{id:"333623",title:"Dr.",name:"Mauricio",surname:"Homem-de-Mello",slug:"mauricio-homem-de-mello",fullName:"Mauricio Homem-de-Mello"},{id:"333838",title:"BSc.",name:"Giulia Oliveira",surname:"Timo",slug:"giulia-oliveira-timo",fullName:"Giulia Oliveira Timo"},{id:"333839",title:"BSc.",name:"Adriana",surname:"Françozo de Melo",slug:"adriana-francozo-de-melo",fullName:"Adriana Françozo de Melo"}],corrections:null},{id:"74793",title:"Phytochemical Antioxidants: Past, Present and Future",doi:"10.5772/intechopen.95627",slug:"phytochemical-antioxidants-past-present-and-future",totalDownloads:432,totalCrossrefCites:3,totalDimensionsCites:5,hasAltmetrics:0,abstract:"Most diseases that are difficult to prevent and cure are “syndromes” that are governed by multiple components with complicated interactions. Whatever the cause of such diseases, overproduction of harmful reactive oxygen species (ROS) can often be observed in progression of the disease. Under such conditions, the cells may be challenged by “oxidative stress” due to excessively generated oxidants. Antioxidants can be defined as chemical compounds that scavenge ROS or free radicals over-produced in the cells under oxidative stress conditions. The plant pigments flavonoids and betalains, rich in fruits and vegetables, are reactive not only with ROS but also with reactive nitrogen species (RNS) and possibly with reactive sulfur species (RSS). Here, we provide an overview of updates on the antioxidative functions of the plant pigments along with some prospects for future research on phytochemical antioxidants.",signatures:"Yasuko Sakihama and Hideo Yamasaki",downloadPdfUrl:"/chapter/pdf-download/74793",previewPdfUrl:"/chapter/pdf-preview/74793",authors:[{id:"335547",title:"Prof.",name:"Hideo",surname:"Yamasaki",slug:"hideo-yamasaki",fullName:"Hideo Yamasaki"},{id:"345030",title:"Dr.",name:"Yasuko",surname:"Sakihama",slug:"yasuko-sakihama",fullName:"Yasuko Sakihama"}],corrections:null},{id:"77906",title:"Broad Efficacy of Scavenging Free Radicals: Cordyceps sp.",doi:"10.5772/intechopen.99405",slug:"broad-efficacy-of-scavenging-free-radicals-em-cordyceps-em-sp-",totalDownloads:171,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Scavenging free radical potency of cordycepin is the major bioactive segment extricated from Cordyceps species. In some new years, Cordyceps has gotten growing thought inferable from its distinctive restorative/pharmacological tests. This assessment reviews continuous explores on the counter oxidant impacts and the associated analyses of Cordyceps species. The results from our review show that Cordyceps of the cordycepin applies protective effects against hostile to oxidant injury for certain, afflictions including constant obstructive pneumonic infection (COPD), hepatitis, asthma, cerebral paralysis, Parkinson’s illness (PD), coronary course sickness (CAD), Alzheimer illness, respiratory failure, malignancy infection, maturing, waterfalls, and mind brokenness. Cordyceps coordinates the NF-κB, RIP2/Caspase-1, Akt/GSK-3β/p70S6K, TGF-β/Smads, and Nrf2/HO-1 hailing pathways among others of cordycepin. A couple of assessments focusing in on Cordyceps auxiliaries were surveyed and found to down metabolic speed of Cordyceps and augmentation its bioavailability. In addition, cordycepin further developed opposition, prevented the duplication of viral RNA, and covered cytokine storms, therefore proposing its capacity to treat COVID-19 and other viral defilements. From the accumulated and assessed information, this article gives the speculative reason to the clinical usages of cordycepin and inspects the way for future assessments focusing in on expanding the restorative use of Cordyceps species. Cordycepin and its analogs show unfathomable potential as the accompanying new class of against oxidant specialists.",signatures:"Loknath Deshmukh, Rajendra Singh and Sardul Singh Sandhu",downloadPdfUrl:"/chapter/pdf-download/77906",previewPdfUrl:"/chapter/pdf-preview/77906",authors:[{id:"334920",title:"Dr.",name:"Loknath",surname:"Deshmukh",slug:"loknath-deshmukh",fullName:"Loknath Deshmukh"},{id:"427631",title:"Dr.",name:"Rajendra",surname:"Singh",slug:"rajendra-singh",fullName:"Rajendra Singh"},{id:"427632",title:"Dr.",name:"Sardul Singh",surname:"Sandhu",slug:"sardul-singh-sandhu",fullName:"Sardul Singh Sandhu"}],corrections:null},{id:"74191",title:"One Health and the Positive Effects of Alaskan Blueberries",doi:"10.5772/intechopen.94738",slug:"one-health-and-the-positive-effects-of-alaskan-blueberries",totalDownloads:106,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"One Health is a principle that takes into account the interactions of humans, animals, the surrounding environment, and how they affect each other. In order to examine this concept in an experimental paradigm, the effects and benefits of wild Alaskan blueberries were compared to those from the continental United States (Lower-48 states) in human and animal studies. Blueberries have been hailed as a superfood for years now due to their high antioxidant levels and the positive effects they have on cardiovascular health and overall health and well-being. We hypothesize that although they are both beneficial, wild Alaskan blueberries have a greater positive effect on health and well-being than those from the lower 48. First, teachers and staff at the Anne Wien Elementary School in Fairbanks Alaska were provided with Alaskan and Lower-48 blueberries and asked to log the effects each coded sample had on their mental and physical health compared to a 5-day control period without blueberries. There was a significant stepwise positive effect of respondents reporting higher self-ratings of well-being overall. Alaskan blueberries significantly improved self-ratings of well-being compared to those from Lower-48 blueberries, albeit those blueberries did improve well-being compared to no blueberries. This experiment was replicated at a control site contemporaneously. The following year it was also replicated by participating teachers and staff at William S. Hackett Middle School in Albany, New York, as well as a control site. Further, lab rats, whose diets were supplemented with with Alaskan blueberries, performed better in exploratory and cognitive tests than did rats whose diets were supplemented with Lower-48 blueberries (who, similar to the previous trials, performed better than rats whose diets had not been supplemented at all). These findings suggest that blueberries have an overall positive effect on self-rated wellness in people and cognitive performance in lab rats and that Alaskan blueberries have a particularly greater and more beneficial effect. Whether this is due to greater antioxidant effects associated with higher altitude or fewer endocrine-disrupting contaminants in Alaska compared to the Lower-48 States is unknown and subject to ongoing investigation.",signatures:"Vincent F. Lembo and Cheryl A. Frye",downloadPdfUrl:"/chapter/pdf-download/74191",previewPdfUrl:"/chapter/pdf-preview/74191",authors:[{id:"324395",title:"Prof.",name:"Cheryl A.",surname:"Frye",slug:"cheryl-a.-frye",fullName:"Cheryl A. Frye"},{id:"324396",title:"Mr.",name:"Vincent F.",surname:"Lembo",slug:"vincent-f.-lembo",fullName:"Vincent F. Lembo"}],corrections:null},{id:"75581",title:"Antioxidant Properties of Alpine Plant",doi:"10.5772/intechopen.96735",slug:"antioxidant-properties-of-alpine-plant",totalDownloads:241,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:"Alpines are the exceptional regions of the world’s biomes. They have unique climatic and topographic conditions; they are the habitat of some of the highly specialized flora and fauna. The harsh environmental conditions and extreme fluctuations in them on a seasonal and diurnal basis created extremely stressful situations for the alpine plants. Such stress causes damage to biochemical structures and compounds of the plant cells leads to the production of free radicals, i.e. reactive oxygen species (ROS), which can further damage the plant cells or tissues. Alpine plants protect themselves from those ROS efficiently by their highly competent enzymatic and non-enzymatic antioxidant system. In general, this protection increases in alpine plants with altitudes; however, some exceptions are also reported. Antioxidant compounds viz. ascorbic acid, tocopherol, glutathione, carotenoids found in alpine flora in higher concentrations as compared to low land plants. Phenolic compounds protected the alpine plants from UV induced ROS by screening the UV radiations and blocking their entries in the cell’s mesophyll. This higher antioxidant potential of the alpine plants is highly beneficial for the human being as most alpine plants are the sources of some life-saving drugs.",signatures:"Vijay Laxmi Trivedi and Mohan Chandra Nautiyal",downloadPdfUrl:"/chapter/pdf-download/75581",previewPdfUrl:"/chapter/pdf-preview/75581",authors:[{id:"333301",title:"Assistant Prof.",name:"Vijay Laxmi",surname:"Trivedi",slug:"vijay-laxmi-trivedi",fullName:"Vijay Laxmi Trivedi"},{id:"349378",title:"Prof.",name:"Mohan Chandra",surname:"Nautiyal",slug:"mohan-chandra-nautiyal",fullName:"Mohan Chandra Nautiyal"}],corrections:null},{id:"75226",title:"Use of Selected Antioxidant-Rich Spices and Herbs in Foods",doi:"10.5772/intechopen.96136",slug:"use-of-selected-antioxidant-rich-spices-and-herbs-in-foods",totalDownloads:457,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Free radicals are chemicals that play a role in the etiopathogenesis of ischemia–reperfusion injury. To prevent or reduce this damage, many protective or therapeutic antioxidants are used effectively in alternative medicine. These antioxidants include immunological or pharmacological agents, vitamins, food and herbal products, and spices. Herbs and spices have been used for a long time as coloring or preservative agents by adding to the content of foods, and at the same time to increase the nutritional value of foods. More recently, the nutritional effects of herbs and spices have become more perceived and the area of interest for these products has increased. Concordantly, the biological contents of herbs and spices have begun to be studied in more detailed way at the cellular and molecular level. Sample plants are classified according to different chemical families, with the diet. Therefore, they have different levels of antioxidant capacity. These products also have potent anti-inflammatory, antihypertensive, glucoregulatory, antithrombotic, anticarcinogenic and so forth effects. These properties are used in the treatment of some chronic diseases. In this review, the antioxidant properties of various herbs and spices used to add flavor to foods or to extend their shelf life have been examined in the light of large-scale nutritional epidemiological studies, in vitro cellular/animal studies and clinical trials.",signatures:"Perçin Karakol and Emin Kapi",downloadPdfUrl:"/chapter/pdf-download/75226",previewPdfUrl:"/chapter/pdf-preview/75226",authors:[{id:"297745",title:"Dr.",name:"Perçin",surname:"Karakol",slug:"percin-karakol",fullName:"Perçin Karakol"},{id:"346058",title:"Dr.",name:"Emin",surname:"Kapi",slug:"emin-kapi",fullName:"Emin Kapi"}],corrections:null},{id:"76481",title:"Cinnamomum zeylanicum: Morphology, Antioxidant Properties and Bioactive Compounds",doi:"10.5772/intechopen.97492",slug:"-em-cinnamomum-zeylanicum-em-morphology-antioxidant-properties-and-bioactive-compounds",totalDownloads:403,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Cinnamomum zeylanicum is one of the oldest spices used for culinary purposes in Asian countries. Its extracts have demonstrated a positive impact on controlling the progression of disease pathologies due to antioxidant, anti-inflammatory, antimicrobial, anticancer, anti-mutagenic, anti-tyrosinase and antidiabetic characteristics. C. zeylanicum also has its unique variations which makes it necessary to distinguish it from other species of cinnamon. Phenolic compounds such as cinnamaldehyde, eugenol, carvacrol, cinnamic acetate and thymol are the main compounds that can be found in essential oils of C. zeylanicum. However, cinnamaldehyde and eugenol act as the main bioactive antioxidant compounds found in C. zeylanicum because of their active functional groups in the structures. There are many examples of the use of C. zeylanicum extracts for medicinal purposes, specifically cinnamon metabolite proanthocyanidins which suppress inflammatory compounds and help pathways such as insulin signaling. Moreover, the bioactive compounds in essential oils of this plant are used against many pathogenic (including food-borne) and spoilage bacteria.",signatures:"Asel Chandula Weerasekera, Kanchana Samarasinghe, Heethaka Krishantha Sameera de Zoysa, Thushara Chathuranga Bamunuarachchige and Viduranga Yashasvi Waisundara",downloadPdfUrl:"/chapter/pdf-download/76481",previewPdfUrl:"/chapter/pdf-preview/76481",authors:[{id:"194281",title:"Dr.",name:"Viduranga Y.",surname:"Waisundara",slug:"viduranga-y.-waisundara",fullName:"Viduranga Y. Waisundara"},{id:"309844",title:"Dr.",name:"Asel C.",surname:"Weerasekara",slug:"asel-c.-weerasekara",fullName:"Asel C. Weerasekara"},{id:"320764",title:"B.Sc.",name:"Kanchana",surname:"Samarasinghe",slug:"kanchana-samarasinghe",fullName:"Kanchana Samarasinghe"},{id:"328152",title:"Mr.",name:"Heethaka Krishantha Sameera",surname:"de Zoysa",slug:"heethaka-krishantha-sameera-de-zoysa",fullName:"Heethaka Krishantha Sameera de Zoysa"},{id:"348620",title:"Dr.",name:"Thushara Chathuranga",surname:"Bamunuarachchige",slug:"thushara-chathuranga-bamunuarachchige",fullName:"Thushara Chathuranga Bamunuarachchige"}],corrections:null},{id:"77885",title:"Dietary Phytochemicals: As a Natural Source of Antioxidants",doi:"10.5772/intechopen.99159",slug:"dietary-phytochemicals-as-a-natural-source-of-antioxidants",totalDownloads:249,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Since time immemorial, plants are used as the source of food and medicine. It can be traced back to the start of humanity. Bringing plant-based food, such as fruits, vegetables, and whole grains, rich in phytochemicals, with beneficial nutrients, opens the door for healthy living. The health benefits are partly attributed to the compounds which possess antioxidants. Several epidemiological observations have shown an opposite relationship between consumption of plant-based foods, rich in phytochemicals, and many diseases including cancer. The majority of the ailments are related to oxidative stress induced by free radicals. Free radicals are extremely unstable with a very short half-life, highly reactive molecule which leads to oxidative damage to macromolecules such as proteins, DNA, and lipids. Free radical induced cellular inflammation appears to be a major contributing factor to cause aging, and degenerative diseases such as cancer, cardiovascular diseases, diabetes, hepatic diseases, renal ailments, and brain dysfunction. Free radicals have been caught up in the pathogenesis of several diseases. Providentially, free radical formation is controlled naturally by phytochemicals, through their antioxidant potential which plays a key role in preventing many diseases including cancer by suppressing oxidative stress-induced DNA damage. Keeping these facts in mind, an attempt has been made to highlight the oxidative stress, enzymatic and non-enzymatic antioxidant, dietary phytochemicals and their role of in disease prevention and cure.",signatures:"Manju Singh Makhaik, Arvind K. Shakya and Raosaheb Kale",downloadPdfUrl:"/chapter/pdf-download/77885",previewPdfUrl:"/chapter/pdf-preview/77885",authors:[{id:"139126",title:"Dr.",name:"Manju",surname:"Singh Makhaik",slug:"manju-singh-makhaik",fullName:"Manju Singh Makhaik"},{id:"210118",title:"Dr.",name:"Arvind K.",surname:"Shakya",slug:"arvind-k.-shakya",fullName:"Arvind K. Shakya"},{id:"423608",title:"Prof.",name:"Raosaheb",surname:"Kale",slug:"raosaheb-kale",fullName:"Raosaheb Kale"}],corrections:null},{id:"75709",title:"Biosynthesis and Regulation of Antioxidant Flavonolignans in Milk Thistle",doi:"10.5772/intechopen.96783",slug:"biosynthesis-and-regulation-of-antioxidant-flavonolignans-in-milk-thistle",totalDownloads:202,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Mature fruits (achenes) of milk thistle (Silybum marianum (L.) Gaertner, Asteraceae) accumulate high amounts of silymarin, a complex mixture of bioactive antioxidant flavonolignans deriving from taxifolin. Their biological activities in relation with human health promotion and disease prevention have been well described. The conditions of their biosynthesis in planta, however, have long been elusive and thus tend to be a limiting factor for their future applications. Significant advances in understanding their biosynthesis and regulation have been made over the last decade and are outlined in the current chapter.",signatures:"Samantha Drouet and Christophe Hano",downloadPdfUrl:"/chapter/pdf-download/75709",previewPdfUrl:"/chapter/pdf-preview/75709",authors:[{id:"313856",title:"Dr.",name:"Christophe",surname:"Hano",slug:"christophe-hano",fullName:"Christophe Hano"},{id:"332653",title:"Dr.",name:"Samantha",surname:"Drouet",slug:"samantha-drouet",fullName:"Samantha Drouet"}],corrections:null},{id:"74748",title:"Reappraisal of Dietary Phytochemicals for Coronavirus Infection: Focus on Hesperidin and Quercetin",doi:"10.5772/intechopen.95529",slug:"reappraisal-of-dietary-phytochemicals-for-coronavirus-infection-focus-on-hesperidin-and-quercetin",totalDownloads:1195,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:1,abstract:"Food polyphenols constitute a large family of substances with beneficial properties in a large group of communicable and non-communicable diseases. These compounds support and improve the body’s defences against oxidative stress and are helpful in the prevention of pathologies related to metabolic syndrome. Furthermore, they exhibit anti-inflammatory, antiviral, and antimicrobial properties. This chapter draws attention to certain nutritional components such as hesperidin and quercetin, which are emerging as good candidates for a complementary beneficial effect in the case of diseases caused by viruses, including COVID-19. These nutraceuticals have a complex mechanism of action, which involves both cellular defence against oxidative stress and the modulation of inflammation, which although normally is a defence, repair and activation mechanism of the immune system, it can elude its controls and become a systemic and destructive pathology (cytokine storm, respiratory distress syndrome). Furthermore, recent in silico simulation tests suggest that both hesperidin and quercetin may interfere with SARS-CoV-2 by binding to cell receptors and the proteolytic enzymes involved in its replication. In addition to the inhibitory effects on the virus at cellular level, the two flavonoids can have indirect effects in respiratory infectious diseases as they prevent or improve metabolic and vascular comorbidities that can complicate the clinical course. This brief review focuses on biochemical and pharmacological mechanisms of action of polyphenols in the context of the revaluation of dietary approaches to the prevention and treatment of infectious diseases caused by viruses, with a special application to COVID-19.",signatures:"Paolo Bellavite",downloadPdfUrl:"/chapter/pdf-download/74748",previewPdfUrl:"/chapter/pdf-preview/74748",authors:[{id:"333914",title:"M.D.",name:"Paolo",surname:"Bellavite",slug:"paolo-bellavite",fullName:"Paolo Bellavite"}],corrections:null},{id:"74753",title:"Evolutionary Strategies of Highly Functional Catalases for Adaptation to High H2O2 Environments",doi:"10.5772/intechopen.95489",slug:"evolutionary-strategies-of-highly-functional-catalases-for-adaptation-to-high-h-sub-2-sub-o-sub-2-su",totalDownloads:195,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Enzymatic evolutionary strategies for adaptation to a high H2O2 environment have been evaluated using catalases with high catalytic efficiency isolated from two H2O2-tolerant bacteria, Exiguobacterium oxidotolerans and Psychrobacter piscatori. The entrance size of the narrow main channel in catalase has been estimated by determining the formation rate of the intermediate state of peracetic acid (b), which is a larger substrate than H2O2 versus that of catalase activity with H2O2 (a) (calculated as b/a). The ratio of b/a in E. oxidotolerans catalase (EKTA) is much higher than that of P. piscatori catalase (PKTA). To elucidate the structural differences between the catalases, the amino acids present in the main channel have been compared between the two catalases and other catalases in the database. The combination of amino acid residues, which contribute high catalytic efficiency in the narrow main channel of EKTA were different from those in PKTA. In this review, we discuss strategic differences in the elimination of high concentration of H2O2 owing to differences in the phylogenetic positions of catalases. In addition, we describe the relationships between the environmental distributions of genera involved in H2O2-resistant bacteria and their catalase functions based on the main channel structure of catalase.",signatures:"Isao Yumoto, Yoshiko Hanaoka and Isao Hara",downloadPdfUrl:"/chapter/pdf-download/74753",previewPdfUrl:"/chapter/pdf-preview/74753",authors:[{id:"337069",title:"Dr.",name:"Isao",surname:"Yumoto",slug:"isao-yumoto",fullName:"Isao Yumoto"},{id:"337070",title:"Dr.",name:"Isao",surname:"Hara",slug:"isao-hara",fullName:"Isao Hara"},{id:"337071",title:"Ms.",name:"Yoshiko",surname:"Hanaoka",slug:"yoshiko-hanaoka",fullName:"Yoshiko Hanaoka"}],corrections:null},{id:"75748",title:"Antioxidant Properties of Metabolites from New Extremophiles Microalgal Strain (Southern, Tunisia)",doi:"10.5772/intechopen.96777",slug:"antioxidant-properties-of-metabolites-from-new-extremophiles-microalgal-strain-southern-tunisia-",totalDownloads:212,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"With the demand for bioproducts that can provide benefits for biotechnology sectors like pharmaceuticals, nutraceuticals, and cosmeceuticals, the exploration of microalgal products has turned toward extremophiles. This chapter is intended to provide an insight to most important molecules from halotolerant species, the cyanobacteria Phormidium versicolor NCC-466 and Dunaliella sp. CTM20028 isolated from Sfax Solar Saltern (Sfax) and Chott El-Djerid (Tozeur), Tunisia. These microalgae have been cultured in standard medium with a salinity of 80 PSU. The in vitro antioxidant activities demonstrated that extremolyte from Dunaliella and Phormidium as, phycocaynin, lipids, and polyphenol compound presents an important antioxidant potential.",signatures:"Sana Gammoudi, Ines Dahmen-Ben Moussa, Neila Annabi-Trabelsi, Habib Ayadi and Wassim Guermazi",downloadPdfUrl:"/chapter/pdf-download/75748",previewPdfUrl:"/chapter/pdf-preview/75748",authors:[{id:"254956",title:"Prof.",name:"Habib",surname:"Ayadi",slug:"habib-ayadi",fullName:"Habib Ayadi"},{id:"335982",title:"Assistant Prof.",name:"Wassim",surname:"Guermazi",slug:"wassim-guermazi",fullName:"Wassim Guermazi"},{id:"336663",title:"Dr.",name:"Sana",surname:"Gammoudi",slug:"sana-gammoudi",fullName:"Sana Gammoudi"},{id:"336664",title:"Dr.",name:"Ines",surname:"Dahmen-Ben Moussa",slug:"ines-dahmen-ben-moussa",fullName:"Ines Dahmen-Ben Moussa"},{id:"348798",title:"Dr.",name:"Neila",surname:"Annabi-Trabelsi",slug:"neila-annabi-trabelsi",fullName:"Neila Annabi-Trabelsi"}],corrections:null},{id:"74893",title:"Endogenous Enzymatic Antioxidant Defense and Pathologies",doi:"10.5772/intechopen.95504",slug:"endogenous-enzymatic-antioxidant-defense-and-pathologies",totalDownloads:344,totalCrossrefCites:3,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Oxidative stress is an important component of various diseases. It manifests as an imbalance caused by an excessive production of reactive oxygen species (ROS) which are associated with a deficit of antioxidant activity. This deficit can be the consequence of genetic factors, environmental ones, metabolic imbalance, toxicity or direct attacks by the accumulation of free radicals. These can induce metabolic dysfunction affecting biological macromolecules in their structures or activities. From a physiological perspective, the neutralization of free radicals is ensured by enzymatic, antioxidant and non-enzymatic defense systems. In the present chapter, we will focus on the endogenous enzymatic antioxidant defense system such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPxs), thioredoxin (Trx) and paraxonase which play an important role in homeostatic redox balance. Also, we will review this set of antioxidants enzymes within different pathological states such as diabetes, cancer, autoimmune diseases, cardiovascular, Alzheimer’s, Parkinson’s or parasitic diseases such as Leishmaniasis and Malaria.",signatures:"Atika Eddaikra and Naouel Eddaikra",downloadPdfUrl:"/chapter/pdf-download/74893",previewPdfUrl:"/chapter/pdf-preview/74893",authors:[{id:"277879",title:"Dr.",name:"Atika",surname:"Eddaikra",slug:"atika-eddaikra",fullName:"Atika Eddaikra"},{id:"334441",title:"Dr.",name:"Naouel",surname:"Eddaikra",slug:"naouel-eddaikra",fullName:"Naouel Eddaikra"}],corrections:null},{id:"74678",title:"Role of Secondary Metabolites to Attenuate Stress Damages in Plants",doi:"10.5772/intechopen.95495",slug:"role-of-secondary-metabolites-to-attenuate-stress-damages-in-plants",totalDownloads:461,totalCrossrefCites:4,totalDimensionsCites:6,hasAltmetrics:0,abstract:"Plants are constantly facing various threats posed by the biotic and abiotic stressors. To survive in these challenged environment, plants evolve a variety of defense mechanism. Among the various phytochemicals, secondary metabolites (SMs) accumulate higher amount under stressful conditions and initiate signaling functions to up-regulation of defense responsive genes. SMs ensures the survival, persistence and competitiveness of the plant against the threat generated under stressful conditions. Therefore, the signaling functions of SMs to protect the plant from biotic and abiotic stressors are getting importance in the recent times. In this chapter the contribution of SMs to protect the plant from specific environmental stresses has been discussed.",signatures:"Masuma Zahan Akhi, Md. Manjurul Haque and Md. Sanaullah Biswas",downloadPdfUrl:"/chapter/pdf-download/74678",previewPdfUrl:"/chapter/pdf-preview/74678",authors:[{id:"334804",title:"Dr.",name:"Md Sanaullah",surname:"Biswas",slug:"md-sanaullah-biswas",fullName:"Md Sanaullah Biswas"},{id:"334900",title:"Dr.",name:"Masuma",surname:"Akhi",slug:"masuma-akhi",fullName:"Masuma Akhi"},{id:"334906",title:"Dr.",name:"Md. Manjurul",surname:"Haque",slug:"md.-manjurul-haque",fullName:"Md. Manjurul Haque"}],corrections:null},{id:"75146",title:"Valorization of Natural Antioxidants for Nutritional and Health Applications",doi:"10.5772/intechopen.96111",slug:"valorization-of-natural-antioxidants-for-nutritional-and-health-applications",totalDownloads:318,totalCrossrefCites:2,totalDimensionsCites:4,hasAltmetrics:1,abstract:"The significant increase in the world population age, 47 years in 1950 to 73 years in 2020, resulted in an increase in aging related diseases as well as in degenerative diseases. In consequence, researchers have been focusing in the development of new therapies, with a particular emphasis on the use of compounds with antioxidant properties, namely phytochemicals, such as polyphenols and carotenoids. Several in vitro and in vivo studies have demonstrated the phytochemicals antioxidant capacity. Their use is broad, as they can be part of food supplements, medicine and cosmetics. The health benefit of antioxidant phytochemicals is an indisputable question. Phytochemical properties are highly influenced by the natural matrix as well as by extraction process, which have a key role. There are several extraction methods that can be applied depending on the chemical properties of the bioactive compounds. There is a wide range of solvents with different polarities, which allows a selective extraction of the desired target family of compounds. Greener technologies have the advantage to reduce extraction time and solvent quantity in comparison to the most traditional methods. This chapter will focus on the different green extraction strategies related to the recovery of antioxidant bioactive compounds from natural sources, their nutritional and health potential.",signatures:"Pedro Ferreira-Santos, Zlatina Genisheva, Claudia Botelho, Cristina Rocha and José António Teixeira",downloadPdfUrl:"/chapter/pdf-download/75146",previewPdfUrl:"/chapter/pdf-preview/75146",authors:[{id:"109523",title:"Prof.",name:"José António",surname:"Teixeira",slug:"jose-antonio-teixeira",fullName:"José António Teixeira"},{id:"258963",title:"Dr.",name:"Claudia Manuela",surname:"Da Cunha Ferreira Botelho",slug:"claudia-manuela-da-cunha-ferreira-botelho",fullName:"Claudia Manuela Da Cunha Ferreira Botelho"},{id:"335595",title:"Dr.",name:"Pedro",surname:"Ferreira-Santos",slug:"pedro-ferreira-santos",fullName:"Pedro Ferreira-Santos"},{id:"335596",title:"Dr.",name:"Cristina",surname:"Rocha",slug:"cristina-rocha",fullName:"Cristina Rocha"},{id:"345948",title:"Dr.",name:"Zlatina",surname:"Genisheva",slug:"zlatina-genisheva",fullName:"Zlatina Genisheva"}],corrections:null},{id:"75789",title:"Recent Advances in Antioxidant Capacity Assays",doi:"10.5772/intechopen.96654",slug:"recent-advances-in-antioxidant-capacity-assays",totalDownloads:582,totalCrossrefCites:5,totalDimensionsCites:9,hasAltmetrics:0,abstract:"This work presents a survey of the important antioxidant capacity/activity assays applied for a diversity of samples including plant extracts, foods, biological material, etc. The published materials are critically discussed, emphasizing the recent findings in the field. New and emergent antioxidant capacity assays, such as nanoparticles-based assay, are also presented. The discussion includes chemical-based methods as well as biochemical and cellular assays. Chemical methods detailed are radical/ROS-based scavenging assays (the trolox equivalent antioxidant capacity (TEAC/ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC) assays, chemiluminescence methods, total radical-trapping antioxidant parameter (TRAP), total oxy radical scavenging capacity (TOSC), and β-carotene bleaching assays), non-radical redox potential-based assays (ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), nanoparticle-based methods and electrochemical methods), metal chelation capacity and total phenolic content tests. The biochemical-based assays and in vivo assays discussed include the oxidation of low density lipoprotein (LDL), the thiobarbituric acid reactive substances (TBARS) and the cellular antioxidant activity (CAA) assays. While a direct link between the antioxidant capacity and health benefits is still a matter of debate, the antioxidant testing methodologies presented in this chapter remain valuable for the high efficiency and cost-effective evaluation of antioxidants, from compound discovery to quality control.",signatures:"Andrei Florin Danet",downloadPdfUrl:"/chapter/pdf-download/75789",previewPdfUrl:"/chapter/pdf-preview/75789",authors:[{id:"332796",title:"Emeritus Prof.",name:"Andrei Florin",surname:"Danet",slug:"andrei-florin-danet",fullName:"Andrei Florin Danet"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"10111",title:"Apolipoproteins, Triglycerides and Cholesterol",subtitle:null,isOpenForSubmission:!1,hash:"29ed0d776c8e3b2af0e50b3c4cf5e415",slug:"apolipoproteins-triglycerides-and-cholesterol",bookSignature:"Viduranga Y. Waisundara and Miljana Z. 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\r\n\tThe goal of this book is to give the reader an overview of a field related to various applications in chemistry, chemical engineering, and nanotechnology. This book aims to provide information about the design of ion exchangers, their application in environmental technologies, and in biotechnology and pharmaceutical applications. This book will be written by authors in the field of experimental methods and critical reviews from multi-disciplines such as chemistry, membranes, and materials science. Among others, some of the topics covered will be Structure of ion exchangers, Synthesis of ion exchangers, Synthesis of inorganic ion exchangers, Properties of ion exchangers, Ion exchange voltammetry, Ion exchange as a separations method, Ion exchange in analytical chemistry, Ion exchange and extraction, Ion exchange membranes, Preparation of organic-inorganic hybrid ion exchangers, Application in environmental technologies, Application in biotechnology and pharmaceutical applications.
\r\n
\r\n\tIn this book, the authors will focus on recent studies, applications, and new technological developments on the fundamental properties of ion exchangers.
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1. Introduction: Concepts, Purposes, and Approaches
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This development responds to a challenge. Text mining software can conveniently generate very large sets of terms or phrases. Our examples draw from use of VantagePoint (or equivalently, Thomson Data Analyzer – TDA) software [1] to analyze abstract record sets. A typical search on an ST&I topic of interest might yield, say, 5,000 records. One approach is to apply VantagePoint’s Natural Language Processing (NLP) to the titles, and also to the abstracts and/or claims. We also take advantage of available topic-rich fields such as keywords and index terms. Merging these fields could well offer on the order of 100,000 terms and phrases in one field (list). That list, unfortunately, will surely contain much noise and redundancy. The text clumping aim is to clean and consolidate such a list to provide rich, usable content information.
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As described, the text field of interest can contain terms (i.e., single words or unigrams) and/or phrases (i.e., multi-word noun + modifiers term sets). Herein, we focus on such NLP phrases, typically including many single words also. Some of the algorithms pertain especially to multi-word phrases, but, in general, many steps can usefully be applied to single-word term sets. Here we focus on analyzing NLP English noun-phrases – to be called simply „phrases.“
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Our larger mission is to generate effective Competitive Technical Intelligence (CTI). We want to answer basic questions of „Who is doing What, Where and When?“ In turn, that information can be used to build „innovation indicators“ that address users‘ CTI needs [2]. Typically, those users might be:
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Information professionals (compiling most relevant information resources)
Researchers (seeking to learn about the nearby „research landscape“)
R&D managers (wanting to invest in the most promising opportunities)
Science, Technology and Innovation (ST&I) policy-makers (striving to advance their country’s competitiveness)
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We focus on ST&I information sets, typically in the form of field-structured abstract records retrieved from topical database searches [e.g., Web of Science (WoS), Derwent World Patent Index, Factiva]. These records usually contain a mix of free text portions (e.g., abstracts) and structured text fields (e.g., keywords, publication years). The software uses an import filter to recognize fields (i.e., to know where and how to find the authors and parse their names properly) for particular source sets, such as WoS. VantagePoint can merge multiple datasets from a given source database or from different sources (with guidance on field matching and care in interpreting).
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\n\t\t\t\tFigure 1 presents our framework for „term clumping.“ We combine established and relatively novel bibliometric and text mining techniqueswithin this framework. Itincludesa number of steps to process alarge phrase list. The top portion of the figure indicates choices to be made concerning which data resources to mine and selection criteria for the records to be analyzed. The next tier notes additional choices regarding which content-laden fields to process. The following two blocks contain the major foci of this chapter. “Text Cleanup” includes stopword and common term handling, through several steps to consolidate related terms. “Consolidateion of terms into informative topical factors” follows. Here we treat basic “inductive methods.” The elements of the Figure flagged with an asterisk (*) are addressed in depth herein.
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\n\t\t\t\tFigure 1 also points towardinterests for future work. These include“purposive methods,” wherein our attention focuses on particular terms based on external criteria – e.g., semantic TRIZ (Theory of Inventive Problem Solving) suggests vital functions and actions indicative of technological innovative potential [3, 4]. The idea is to search the target text fields for occurrences of theory-guided terms and adjacent content.
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We are also keenly interested in pursuing single word analyses via Topic Modeling (TM) methods to get at themes of the record set under study. These hold appeal in providing tools that will work well in multiple languages and character sets (e.g., Chinese). The main language dependency that we confront is the use of NLP to extract noun phrases (e.g., VantagePoint’s NLP is developed for English text).
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The bottom portion of Figure 1 indicates interest in how best to engage experts in such topic identification processes. We distinguish three roles:
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Analyst: Professionals in data retrieval and analysis, who have analytical skills in handling text, but usually don’t have domain knowledge
Expert: Professional researchers in the specific domain, knowledgeable over the domain, and able to describe the current status of the domain at both macro and micro levels;
Information & Computer Scientist: Covering a range of skills from in-depth programming, through preparation of macros, to operating software to accomplish particular text manipulations.
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So defined, engagement of experts presents challenges in terms of motivation, time required, and communication of issues so that the domain experts can readily understand and respond to the analyst’s needs. Simple, intermediate stage outputs could have value in this regard.
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Figure 1.
Term Clumping for Technical Intelligence
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In summary, this chapter addresses how best to clean and consolidate ST&I phrase lists from abstract record sets. The target is to semi-automate this „inductive“ process (i.e., letting the data speak without predetermined identification of target terms). We aim toward semi-automation because the process should be tailorable to study needs. We are exploring a series of text manipulations to consolidate phrase lists. We are undertaking a series of experiments that vary how technical the content is, which steps are performed, in what sequence, and what statistical approaches are then used to further cluster the phrases or terms. In particular, we also vary and assess the degree of human intervention in the term clumping. That ranges from almost none, to analyst tuning, to active domain expert participation [5-7].
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2. Review of Related Literatures
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Given the scope of Figure 1, several research areas contribute. This chapter does not address the purposive analyses, so we won’t treat literatures on importing index terms, or on TRIZ and Technology RoadMapping (TRM) -- of great interest in suggesting high value terms for CTI analyses.
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Several of the steps to be elaborated are basic. Removal of „stopwords“ needs little theoretical framing. It does pose some interesting analytical possibilities, however. For instance, Cunningham found that the most common modifiers provided analytical value in classifying British science [8]. He conceives of an inverted U shape that emphasizes analyzing moderately high frequency terms -- excluding both the very high frequency (stopwords and commonly used scientific words, that provide high recall of records, but low precision) and low frequency words (suffering from low recall due to weak coverage, but high precision). Pursuing this notion of culling common scientific words, we remove „common words.“ In our analyses we apply several stopword lists of several hundred terms (including some stemming), and a common words in academic/scientific writing thesaurus of some 48,000 terms [9]. We are interested in whether removal of these enhances or, possibly, degrades further analytical steps‘ performance (e.g., Topic Modeling).
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To state the obvious -- not all texts behave the same. Language and the venue for the discourse, with its norms, affect usage and text mining. In particular, we focus on ST&I literature and patent abstracts, with outreach to business and attendant popular press coverage of topics (e.g., the Factiva database). English ST&I writing differs somewhat from „normal“ English in structure and content. For instance, scientific discourse tends to include many technical phrases that should be retained, not parsed into separate terms or part-phrases by NLP. VantagePoint’s NLP routine [1] strives to do that. It also seeks to retain chemical formulas.
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A research community has built around bibliometric analyses of ST&I records over the past 60 or so years, see for instance [10-12]. DeBellis nicely summarizes many facets of the data and their analyses [13]. Our group at Georgia Tech has pursued ST&I analyses aimed especially at generating Competitive Technical Intelligence (CTI) since the 1970’s, with software development to facilitate mining of abstract records since 1993 [1, 2, 14]. We have explored ways to expedite such text analyses, c.f. [15, 16], as have others [17]. We increasingly turn toward extending such „research profiling“ to aid in Forecasting Innovation Pathways (FIP), see for example [18].
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Over the years many techniques have been used to model content retrieved from ST&I text databases. Latent Semantic Indexing (LSI) [19], Principal Components Analysis (PCA), Support Vector Machines (SVM), and Topic Modeling (TM) are among the key methods that have come forth [20].
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PCA is closely related to LSI. Both use Singular Value Decomposition (SVD) to transform the basic terms by documents matrix to reduce ranks (i.e., to replace a large number of terms by a relatively small number of factors, capturing as much of the information value as possible). PCA eigen-decomposes a covariance matrix, whereas LSI does so on the term-document matrix. [See wikipedia for basic statistical manipulations.]
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VantagePoint uses a special variant of PCA developed to facilitate ST&I text analyses (used in the analyses reported here). This PCA routine generates a more balanced factor set than LSI (which extracts a largest variance explaining factor first; then a second that best explains remaining variance, etc.). The VantagePoint factor map routine applies a small-increment Kaiser Varimax Rotation (yielding more attractive results, but running slower, than SPSS PCA in developmental tests). Our colleague, Bob Watts of the U.S. Army, has led development of a more automated version of PCA, with an optimization routine to determine a best solution (maximizing inclusion of records with fewest factors) based on selected parameter settings -- (Principal Components Decomposition – PCD)[21] He has also empirically compared PCD (inductive) results with a deductive approach based on use of class codes [22].
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We apply PCA to term sets to generate co-occurrence based principal components. Because of the familiar use of “clusters,” we also use that terminology, although other clustering approaches can yield different forms (e.g., K-means, hierarchical clustering). This PCA approach allows terms to appear in multiple factors
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We use the concept, „term clumping,“ as quite general – entailing various means of text consolidation (e.g., application of thesauri, fuzzy matching, stemming) with noise removal. Bookstein, Raita, and collegues offer a somewhat more specialized, but related, interpretation pointing toward the aim of condensing terminology to better identify content-bearing words [23-25]. Term clumping addresses text (not document) „clustering.“ Any type of text clustering is based on co-occurrence of words in records (documents). Clustering, in turn, includes many variations plus additional statistical analyses with considerable commonality -- in particular, factor analysis. PCA can be considered as a basic factoring approach; indeed, we call its output principal components „factors. “Similarity among these term grouping approaches arises in that they generally aim to maximize association within clusters and minimize association among clusters. Features to keep in mind include whether terms or phrases being clustered are allowed to be included in multiple clusters or not; whether algorithms yield the same results on rerun or may change (probabilistic methods); and whether useful visualization are generated. Many further variations are available – e.g., hierarchical or non-hierarchical; building up or partitioning down; neural network based approaches (e.g., Kohonen Self-Organizing Maps), and so forth [26]. Research is actively pursuing many refinements, for many objectives, for instance [27]. Our focus is on grouping terms, but we note much complementary activity on grouping documents (based on co-occurrence with particular terms) [26], with special interest in grouping web sites, for instance [28].
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Latent Semantic Indexing (LSI) or Latent Semantic Analysis, is a classical indexing method based on a Vector Space Model that introduces Singular-Value Decomposition (SVD) to uncover the underlying semantic structure in the text set. The key feature of LSI is to map those terms that occur in similar contexts into a smaller “semantic space“ and to help determine the relationships among terms (synonymy and polysemy) [17, 29, 30]. When applied on co-occurrence information for large text sources, there is no need for LSI to import domain literatures or thesauri (what we call „purposive“ or aided text clumping). There are also various extended LSI methods [31]. Researchers are combining LSI with term clumping variations in order to relate synonymous terms from massive content.For example, Maletic and Marcus combine semantic and structural information [32] and Xu et al. seek to associate genes based on text mining of abstracts [30].
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Topic modeling is a suite of algorithms that automatically conforms topical themes from a collection of documents [33, 34]. This stream of research begins with Latent Dirichlet Allocation (LDA), which remains the basic algorithm. Topic modelling is an extended LSI method, that treats association probabilistically. Various topic modeling algorithms extend the basic approach, for example [35-44]. Topic modeling is being applied in many contexts – e.g., NLP extension, sentiment analysis, and topic detection.
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We are pursuing topic modeling in conjunction with our text clumping development in several ways. We are experimenting to assess whether and which term clumping steps can refine term or phrase sets as input into topic modeling to enhance generation of meaningful topics. We also compare topic modeling outputs to alternative processes, especially PCA performed on clumped phrases. We additionally want to assess whether some form of text clumping can be applied after topic modeling to enhance topic interpretability.
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We have also tried, but are not actively pursuing, Key Graph, a kind of visualization technique that treats the documents as a building constructed by a series of ideas and then retrieves these ideas and posts as a summary of original points on the segmentation of a graph [45-47]. Usually, Key Graph has 3 major components: (1) Foundations, which are the sub-graphs of highly associated and frequent terms; (2) Roofs, which are terms highly related to the foundations; and (3) Columns, which are keywords representing the relationships between foundations and roofs.
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We are especially interested in term grouping algorithms to refine large phrase sets through a sequence of steps. These typically begin with noise removal and basic cleaning, and end with some form of clustering of the resulting phrases (e.g., PCA). „In-between“ we are applying several intermediate stage term consolidation tools. Kongthon has pursued an object oriented association rule mining approach [48], with a „concept grouping“ routine [49] and a tree-structured network algorithm that associates text parent-child and sibling relationships [50].
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Courseault-Trumbach devised a routine to consolidate related phrases, particularly of different term lengths based on term commonality [51]. Webb Myers developed another routine to combine authors. The notion was that, say, we have three papers authored by X. Perhaps two of those are co-authored with Y, and one with Z; and Y and Z never appear as authors on another paper without X. In that case, the operation surmises that Y and Z are likely junior authors, and eliminates them so that further author analyses can focus on the senior authors or author team. The macro [available at www.theVantagePoint.com] adds major co-authors into the term name. We incorporate these two routines in the present exercises.
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Lastly, we consider various quality assessment approaches. Given that one generates clustered text in various forms, which are best? We look toward three approaches. First, we want to ask the target users. While appealing, this also confronts issues – e.g., our PCA output „names“ the resulting factors, whereas topic modeling does not. How can we compare these even-handedly? Second are statistical approaches that measure some form of the degree of coherence within clusters vs. among clusters [52]. Third are record assignment tests – to what extent do alternative text clumping and clustering sequences correctly distinguish mixed dataset components? Here we seek both high recall and precision.
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3. Empirical Investigation:Two Case Analyses
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\n\t\t\t\tFigure 1 arrays a wide range of possible term clumping actions. As introduced in the previous sections, we are interested in many of those, but within the scope of this chapter we focus on many of the following steps and comparisons:
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Term Clumping STEPS:
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Fuzzy matching routines
Thesauri to reduce common terms
Human-aided and topic tailored cleaning
Phrase consolidation macro (different lengths)
Pruning of extremely high and low frequency terms
Combine term networks (parent-child) macro
g.TFIDF (Term Frequency Inverse Document Frequency)
Term normalization vs. parent database samples
PCA variations to generate high, medium, and low frequency factors
Topic Modeling
Quality assessment of the resulting factors – comparing expert and statistical means
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We are running multiple empirical comparisons. Here we compare results on two topical datasets:
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“MOT” (for Management of Technology) – 5169 records covering abstract records of the PICMET (Portland International Conference on Management of Engineering and Technology) from 1997 through 2012.
“DSSCs” (for Dye-Sensitized Solar Cells) – 5784 abstract records compiled from searches for 2001-2010 in WoS and in EI Compendex, merged in VantagePoint
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Elsewhere, we elaborate on these analyses in various ways. Substantive interpretations of the topical MOT thrusts based on the human-selected MOT terms are examined over time and regions [55]. Comparisons of three MOT analyses -- 1) 3-tier, semi-automatic PCA extraction, 2) PCA based on human-selected MOT terms, and 3) Topic Modeling of unigrams – found notably different factors extracted. Human quality assessment did not yield a clear favorite, but the Topic Modeling results edged ahead of the different PCA’s [7]. Additional explorations of the WoS DSSC data appear in [6], comparing Topic Modeling and term clumping-to-PCA – finding quite different emphases in the extracted factors. Zhang et al. [54] track through a similar sequence of term clumping steps on the combined WoS-Compendex DSSC dataset.
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Here, we focus on stepping through most of the term clumping operations for these two cases. To avoid undue complexity, we set aside data variations (e.g., stepping through for the WoS DSSC set alone), Topic Modeling comparisons, and quality assessment. As noted, we have done one version of human assessment for the MOT data [7]. We are pursuing additional quality assessments via statistical measures [52] and by comparing how well the alternative analytics are able to separate out record sets from a combination of 7 searches. We also intend to pursue Step h – term normalization based on external (e.g., entire database) frequencies. So, here we treat Steps a-g and i, not Steps h, j, or k.
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\n\t\t\t\tTable 1 provides the stepwise tally of phrases in the merged topical fields undergoing term clumping. It is difficult to balance precision with clarity, so we hope this succeeds. The first column indicates which text analysis action was taken, coresponding to the list of steps just above.The second column shows the results of those actions applied in sequence on the MOT data. Blank cells indicate that particular action was not performed on the MOT (or DSSC) dataset. The last row notes additional human-informed analyses done on the MOT data, but not treated here (to recognize that this is a selective presentation). The third column relates the results of application of the steps to the DSSC data, but here we indicate sequence within the column, also showing the resulting term reduction. [So, the Table shows the Term Clumping Steps in the order performed on MOT; this was arbitrary. It could as well have been ordered by the list (above) or in the order done for DSSC data.]
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Term Clumping Steps
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MOT 5169 PICMET records
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DSSCs 5784 records (WoS+Compendex), 2001-2010
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Field selection
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Title&Abstract NLP phrases
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Title&Abstract NLP phrases + keywords
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Phrases with which we begin
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86014
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90980
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a-1) Apply general.fuz routine
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76398
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Applied 10th, reducing 82701 to 74263
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b-1) Apply stopwords thesaurus
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76105
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Applied 1st, reducing 90980 to 89576) and applied 7th, reducing 85960 to 84511
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b-2) Apply common academic/scientific terms thesaurus
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73232
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Applied 2d, reducing 89576 to 89403; and applied8th, reducing 84511 to 82739
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b-3) multiple tailored cleaning routines -- trash term remover.the; topic variations consolidator.the; DSSC data fuzzy matcher results.the*
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Applied such actions as 3d-6th steps, reducing 89403 to 85960; applied 9th, reducing 82739 to 82701;
f) Apply combine term networks (parent-child) macro
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10513
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Applied 15th, reducing 20172 to 8181
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g) Apply TFIDF
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1999
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Applied 16th, reducing 8181 to 2008
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i) Auto-PCA: highest frequency; 2d highest; 3d highest
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201, 256, 299
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203;214;230
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
PCA factors
\n\t\t\t\t\t\t
9 factors (only top tier)
\n\t\t\t\t\t\t
12 (only top tier)
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
c-3) Tuned phrases to 7164; reviewed 15 factors from 204 top phrases; reran to get final PCA
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t
Table 1.
Term Clumping Stepwise Results
\n\t\t\t
*a compilation of phrase variations that VantagePoint’s “List Cleanup” routine suggested combining [e.g. – various singular and plural variations; hyphenation variations; and similar phrases such as “nanostructured TiO2 films” with “nanostructured TiO2 thin films”]
\n\t\t\t
Some steps are broken out in more detail – e.g., Step a -- Fuzzy matching routines – is split into use of VantagePoint’s general matching routine (a-1) and application of a variant tunedfor this term clumping (a-2). Note also that some steps appear more than once, especially for the DSSC clumping.
\n\t\t\t
For Step b – application of thesauri to remove common terms – we distinguish the use of a modest size stopwords thesaurus (fewer than 300 words) as Step b-1 and the application of the 48,000 term thesaurus of common academic/scientific terms as Step b-2.
\n\t\t\t
Step c -- Human-aided and topic tailored cleaning (Steps c-1, c-2 & c-3) groups a variety of „obvious“ cleaning routines. Our dilemma is whether to eliminate these, to facilitate development of semi-automated routines, or to include them, for easy improvement of the term consolidation? In the MOT term clumping reported in Table 1, we essentially avoid such cleaning. In the DSSC step-through, we include limited iterations of human-aided cleaning to see whether this makes a qualitative difference by the time the progression of steps is completed. [It does not seem to do so.]
\n\t\t\t
Step d -- Phrase consolidation macro – consolidates only a modest percentage of the phrases (as applied here, reducing the phrase count by 2.3% for MOT and by 3.3% for DSSCs), but the improvements appear worthwhile. For instance, combining “Dye-Sensitized Solar Cells” with “Sensitized Solar Cells” can provide important conceptual concentration.
\n\t\t\t
Step e – Pruning – is simply discarding the phrases that appear in only one record. Those would not add to co-occurrence based analyses. The challenge is to sequence pruning after consolidation so that potentially useful topical information is not discarded. Pruning is the overwhelmingly potent step in reducing the term or phrase counts. For MOT, it effects a reduction of 81%; for DSSCs, 64%.
\n\t\t\t
Step f -- Combine term networks (parent-child) – appears a powerful reducer. As discussed, Webb Myers devised this macro to consolidate author sets.We apply the macro to the phrases field, showing sizable reductions for MOT (19.7%) and DSSCs (59.4%). The macro will combine major co-occurring terms in the new phrase name with a “&” between them. It also results in terms that appear in a single record being combined into a single phrase [hence, we perform the Pruning step prior to applying this macro].
\n\t\t\t
Step g – TFIDF – strives to distinguish terms that provide specificity within the sample set.For example, if some form of „DSSC“ appears in nearly every DSSC record, this would not be a high-value term in distinguishing patterns within the dataset. VantagePoint offers three TFIDF routines – A) un-normalized, B) log, and C) square root. We compared these and proceed with the square root term set for DSSCs, whose 2008 terms are all included in sets A or B. Of the 2008 phrases, 1915 are in both A and B (so differences in this regard are small), with 42 in set A and 51 in set B. For the MOT data, B and C yield the same 1999 terms, whereas A yields 2052. Inspection of the distinct terms find the 78 only in sets B & C to appear more substantive than the 131 terms only in set A, so we opt for the 1999 term result.
\n\t\t\t
Step h is included as a place-holder.On the one hand, Step b aims to remove generally common terms.On another, Step g favors more specific terms within the document set being analyzed. With access to full databases or general samples from sources such as WoS, one could sort toward terms or phrases that are relatively unique to the search set.We have not done that here.
\n\t\t\t
At this stage, we have very large, but clumped, phrase sets. In our two cases, these consist of about 2000 phrases. Consider the illustrative „post-TFIDF“ tabulations in Table 2. We believe these offer rich analytical possibilities. For instance, we could scan their introduction and frequency over time to identify „hot“ topics in the field. Or, we could compare organizational emphases across these phrases to advance CTI interests. We might ask technical and/or business experts in the field to scan those 2000 phrases to identify particularly important or novel ones for in-depth analyses.
\n\t\t\t
Steps i and j represent a major „last step“ for these sorts of term analyses. Here we explore select PCA steps; elsewhere, as noted, we pursue Topic Modeling [6, 7]. This factoring (~clustering) step reduces thousands of phrases to tens of phrases. If done accurately, this can be game-changing in terms of opening conceptual insights into topical emphases in the field under study.
\n\t\t\t
VantagePoint’s PCA routine is now applied as Step i. In these cases we have tried to minimize human-aiding, but we explore that elsewhere [6, 7]. We select three top tiers of terms to be subjected to separate Principal Components Analysis. Such selection can be handled by various coverage rules – e.g., terms appearing in at least 1% of the records. In the present exercises, we set thresholds to provide approximately 200 phrases as input to each of three PCA analyses. We run the default requested number of factors to extract – this is the square root of the number of terms submitted. We review the resulting three sets of factors in terms of recall (record coverage) and determine to focus on just the top tier PCA results here. For DSSCs, the top-tier PCA yields 12 factors that cover 98% of the records, whereas the 2d tier factors cover 47% and the 3d tier only 18%.For the MOT analyses, results are comparable – the 9 top-tier factors cover 90% of the records; 2d tier, 36%; 3d tier, 17%. [We have performed additional analyses of these data, exploring various PCA factor sets, including ones in which we perform post-PCA term cleaning based on inspection of initial results., then rerun PCAFor instance, a very high frequency term might be removed, or simple relations handled by refining a thesaurus (e.g., in one result „Data Envelopment Analysis“ and its acronym, DEA, constituted a factor).
\n\t\t\t
Step j is of high interest, and we are exploring several alternative approaches, as mentioned. Here, we just present the high tier set of PCA factors for face validity checks.
\n\t\t
\n\t\t
\n\t\t\t
4. Term Clumping Case Results
\n\t\t\t
Having stepped through multiple term clumping steps, what do we get? One has many reasonable choices as to which term clumping steps to apply, in what sequence. To get a feel for the gains, let’s compare sample results at four Stages:
\n\t\t\t
Initial phrase set
After the term clumping steps up to TFIDF
After TFIDF
After PCA
\n\t\t\t
Referring to Figure 1, the Text Cleaning stage, in general, would be carried out in preparation for nearly all further analyses. We would not anticipate aborting that processing part-way, except in special cases (e.g., as mentioned in Cunningham’s analysis of British science titles). The next stage of consolidating the cleaned and, therefore, partly consolidated phrases, is where interesting choices arrive. Based on the analyses of the MOT and DSSC data, we note the significant effect of selecting the high TFIDF terms. We thus compare the phrase sets at Stage 1 (before cleaning and clumping), Stage 2 (before filtering to the top TFIDF terms), Stage 3 (after TFIDF), and Stage 4 (after applying one of the clustering family of techniques – PCA).
\n\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Stage 1 - Initial
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Stage 2 - Clumped
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Top 10
\n\t\t\t\t\t\t
# Records
\n\t\t\t\t\t\t
# Instances
\n\t\t\t\t\t\t
Top 10
\n\t\t\t\t\t\t
# Records
\n\t\t\t\t\t\t
#Instances
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
study
\n\t\t\t\t\t\t
1177
\n\t\t\t\t\t\t
1874
\n\t\t\t\t\t\t
technology
\n\t\t\t\t\t\t
475
\n\t\t\t\t\t\t
1113
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
results
\n\t\t\t\t\t\t
894
\n\t\t\t\t\t\t
1177
\n\t\t\t\t\t\t
case study
\n\t\t\t\t\t\t
472
\n\t\t\t\t\t\t
931
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
research
\n\t\t\t\t\t\t
792
\n\t\t\t\t\t\t
1050
\n\t\t\t\t\t\t
applicable
\n\t\t\t\t\t\t
444
\n\t\t\t\t\t\t
998
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
development
\n\t\t\t\t\t\t
603
\n\t\t\t\t\t\t
829
\n\t\t\t\t\t\t
knowledge
\n\t\t\t\t\t\t
414
\n\t\t\t\t\t\t
1022
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
analysis
\n\t\t\t\t\t\t
518
\n\t\t\t\t\t\t
690
\n\t\t\t\t\t\t
relationship
\n\t\t\t\t\t\t
356
\n\t\t\t\t\t\t
801
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
One
\n\t\t\t\t\t\t
494
\n\t\t\t\t\t\t
574
\n\t\t\t\t\t\t
competition
\n\t\t\t\t\t\t
303
\n\t\t\t\t\t\t
699
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
innovation
\n\t\t\t\t\t\t
465
\n\t\t\t\t\t\t
800
\n\t\t\t\t\t\t
governance
\n\t\t\t\t\t\t
248
\n\t\t\t\t\t\t
517
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge
\n\t\t\t\t\t\t
412
\n\t\t\t\t\t\t
750
\n\t\t\t\t\t\t
technology manager
\n\t\t\t\t\t\t
241
\n\t\t\t\t\t\t
526
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
process
\n\t\t\t\t\t\t
400
\n\t\t\t\t\t\t
506
\n\t\t\t\t\t\t
literature
\n\t\t\t\t\t\t
227
\n\t\t\t\t\t\t
344
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
industry
\n\t\t\t\t\t\t
399
\n\t\t\t\t\t\t
637
\n\t\t\t\t\t\t
implication
\n\t\t\t\t\t\t
221
\n\t\t\t\t\t\t
327
\n\t\t\t\t\t
\n\t\t\t\t
Table 2.
Stages 1 & 2 – Top 10MOT Phrases
\n\t\t\t
Considering the MOT data first, Table 2 compares the ten most frequent terms or phrases as of Stages 1 and 2.As per Table 1, the clumping and, especially single-record term pruning, has reduced from 86014 to 10513 phrases – an 88% reduction. Table 2 lists the highest frequency terms and phrases based on record coverage. For instance, study appears in 1177 of the 5169 records (23%). The Table also shows instances, and we see that study appears more than once in some records to give a total of 1874 instances. MOT is Management of Technology. That said, the terms and phrases after clumping are somewhat more substantive. As one scans down the Stage 2 set of 10513 phrases, this is even more the case. Our sense is that a topical expert reviewing these to tag a set of themes to be analyzed (e.g., to track trends, or institutional emphases) would definitely prefer the clumped to the raw phrases.
\n\t\t\t\n\t\t\t
In Tables 2-5, we show in how many of the full sample of MOT and DSSC records the particular terms appear. We also show instances (i.e., some terms appear more than once in a record). These just convey the changes in coverage resulting from the various clumping operations applied.
\n\t\t\t
\n\t\t\t\tTable 3 shows the „Top 10“ post-TFIDF terms and phrases, based on TFIDF scores. Recall that the 1999 terms and phrases at this Stage 3 are based on an arbitrary threshold – we sought about 2000. Note that term counts are unchanged for terms present in both Stages 2 & 3. TFIDF is not clumping, but rather screening based on occurrence patterns across the 5169 records.
\n\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Stage 3 - post-TFIDF
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Top 10
\n\t\t\t\t\t\t
# Records
\n\t\t\t\t\t\t
# Instances
\n\t\t\t\t\t\t
SqRt TFIDF value
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Knowledge
\n\t\t\t\t\t\t
414
\n\t\t\t\t\t\t
1022
\n\t\t\t\t\t\t
35.05
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
technology
\n\t\t\t\t\t\t
475
\n\t\t\t\t\t\t
1113
\n\t\t\t\t\t\t
34.59
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
applicable
\n\t\t\t\t\t\t
444
\n\t\t\t\t\t\t
998
\n\t\t\t\t\t\t
33.68
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
relationship
\n\t\t\t\t\t\t
356
\n\t\t\t\t\t\t
801
\n\t\t\t\t\t\t
32.89
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
competition
\n\t\t\t\t\t\t
303
\n\t\t\t\t\t\t
699
\n\t\t\t\t\t\t
32.57
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
innovation technology
\n\t\t\t\t\t\t
200
\n\t\t\t\t\t\t
527
\n\t\t\t\t\t\t
32.42
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
case study
\n\t\t\t\t\t\t
472
\n\t\t\t\t\t\t
931
\n\t\t\t\t\t\t
31.72
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
technology manager
\n\t\t\t\t\t\t
241
\n\t\t\t\t\t\t
526
\n\t\t\t\t\t\t
30.54
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
R&D
\n\t\t\t\t\t\t
191
\n\t\t\t\t\t\t
446
\n\t\t\t\t\t\t
30.25
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Governance
\n\t\t\t\t\t\t
248
\n\t\t\t\t\t\t
517
\n\t\t\t\t\t\t
29.99
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
developed country
\n\t\t\t\t\t\t
179
\n\t\t\t\t\t\t
406
\n\t\t\t\t\t\t
29.43
\n\t\t\t\t\t
\n\t\t\t\t
Table 3.
Stage 3 – Top 10 MOT Phrases based on TFIDF
\n\t\t\t
\n\t\t\t\tTable 4 presents another 10-term sample pair for Stages 1 and 2. Here, we alphabetically sort the phrase lists and arbitrarily take the ten phrases beginning with „knowledge“ or „knowledg“ --i.e., a stem version of the term. Notice that the big consolidation is for the stemmed version of „knowledg,“ for which the record count has gone up a tiny amount (2), whereas the instance count has increased by 272. In general, the term clumping increases term frequencies and consolidates related terms pretty well (but by no means completely).
\n\t\t\t\n\t\t\t
\n\t\t\t\tTable 5 presents the top-tier PCA analysis results. The phrases appearing here tend to be more topically specific than those seen as most frequent at Stages 2 and 3. Only two terms -- „competition“ and „knowledge“ -- happen to be approximately in common. These nine factors pass a face validity check – they seem quite coherent and potentially meaningful to study of the MOT research arena. Naming of the factors is done by VantagePoint, using an algorithm that takes into account relative term loading on the factor and term commonalities among phrases.
\n\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Stage 1 Sample
\n\t\t\t\t\t\t
#R
\n\t\t\t\t\t\t
#I
\n\t\t\t\t\t\t
Stage 2 Sample
\n\t\t\t\t\t\t
#R
\n\t\t\t\t\t\t
#I
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge
\n\t\t\t\t\t\t
412
\n\t\t\t\t\t\t
750
\n\t\t\t\t\t\t
knowledge
\n\t\t\t\t\t\t
414
\n\t\t\t\t\t\t
1022
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge absorption ability KAA
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
knowledge acquisition
\n\t\t\t\t\t\t
6
\n\t\t\t\t\t\t
11
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge access
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
knowledge age
\n\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
10
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge accumulated
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
knowledge asset
\n\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
5
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge accumulation
\n\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
8
\n\t\t\t\t\t\t
knowledge base
\n\t\t\t\t\t\t
14
\n\t\t\t\t\t\t
17
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge accumulation model
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
2
\n\t\t\t\t\t\t
knowledge based competencies
\n\t\t\t\t\t\t
2
\n\t\t\t\t\t\t
3
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge acquisition
\n\t\t\t\t\t\t
6
\n\t\t\t\t\t\t
11
\n\t\t\t\t\t\t
knowledge based economy
\n\t\t\t\t\t\t
21
\n\t\t\t\t\t\t
28
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge acquisition KA
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
knowledge based organizational strategy
\n\t\t\t\t\t\t
2
\n\t\t\t\t\t\t
4
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge acquisition strategies
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
4
\n\t\t\t\t\t\t
knowledge based perspective
\n\t\t\t\t\t\t
3
\n\t\t\t\t\t\t
4
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
knowledge across different sectors
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
1
\n\t\t\t\t\t\t
Knowledge Based Product Models
\n\t\t\t\t\t\t
2
\n\t\t\t\t\t\t
2
\n\t\t\t\t\t
\n\t\t\t\t
Table 4.
Stages 1 & 2 – 10 Sample MOT Phrases
\n\t\t\t
Note: #R = # of Records; #I = # of Instances
\n\t\t\t\n\t\t\t
As mentioned, we have done additional analyses of these data. In another PCA, starting with the 10513 terms (pre-TFIDF), we extracted a high frequency term set (112 terms or phrases appearing in 50-475 records). In addition we extracted a second-tier PCA based on 185 terms appearing in 25-49 records, and a third-tier PCA from 763 terms in 10-24 records. Each set was run using VantagePoint default settings for number of factors, yielding, respectively, 7, 9, and 16 factors. Of the present 9 top-tier factors, 3 show clear correspondence to either top or second-tier factors in the 10513-term based PCA; one shows partial correspondence; 5 are quite distinct. Which factor sets are better? Impressionalistically, the 9 post-TFIDF factors seem reasonable and somewhat superior, but lacking some of the specificity of (7 + 9 + 16 = 32) factors. As noted, we don’t pursue the corresponding post-TFIDF PCA second and third tier factors because their record coverage is low.
\n\t\t\t\n\t\t\t
Examination of DSSC phrase sets shows generally similar progressions as term clumping proceeds.In some respects, results are even more satisfactory with that more technical terminology.In the interest of space, we don’t present tables like Tables 2-4 here.But here’s a synopsis of one fruitful topical concentration within the DSSC phrase list:
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Principle Component (Factor)
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High Loading Phrases
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\n\t\t\t\t\t\t
Managing Supply Chain
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Managing Supply Chain
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Supply Chain
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\n\t\t\t\t\t\t
Nanotechnology
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Nanotechnology
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Commercial
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\n\t\t\t\t\t\t
Competing Technologies
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Competition
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Capability
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Technology Capability
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Global Competition
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Competing Technologies
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Technology Roadmap
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Roadmap
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\n\t\t\t\t\t\t
Technology Roadmap
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\n\t\t\t\t\t\t
Innovation Process
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Innovation Process
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Innovation Activity
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Open Innovation
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Knowledge
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Knowledge
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Knowledge Manager
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Individual
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Knowledge Creation
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\n\t\t\t\t\t\t
New Knowledge
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\n\t\t\t\t\t\t
Share Knowledge
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\n\t\t\t\t\t\t
Project Success
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Project Manager
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\n\t\t\t\t\t\t
Project Success
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\n\t\t\t\t\t\t
Make Decisions
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Make Decisions
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Decision Making Process
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Communication Technology
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ICT
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\n\t\t\t\t\t\t
Communication Technology
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\n\t\t\t\t
Table 5.
Stage 4 – Top Tier MOT PCA Factors and Constituent Phrases
\n\t\t\t
In the initial 90980 term list, there are 807 terms on “electron/electrons/electronic”
In the 8181 term list, there are 119 terms on this
In the 2008 term list, there are 40 terms remaining, such as “electron acceptor,” “electron diffusion,” “electron injection,” “electron transfer,” “electronic structure,” etc.
\n\t\t\t
\n\t\t\t\tTable 6 shows the twelve top-tier DSSC PCA factors and the phrases that load highly on those factors. These results pace a face validity test in that the grouping of terms seems generally sensible. These factors appear to be reasonable candidates for thematic analysis of this solar cell research & development activity.
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Principle Component (Factor)
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High Loading Phrases
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Sol Gel Process
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Sol Gel
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Sol Gel Process
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Gel-Sol Method
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Polymer Electrolyte
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Electrolyte
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Polym
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Ionic Liquid
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Polymer Electrolyte
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Gel Electrolyte
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Electrolyte Liquid
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Ionic Conduction
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Gel Polymer Electrolyte
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Electrolysis
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Gelator
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Poly electrolyte
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Temperature Molten-Salt
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Conduction Band
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Electron Injection
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Conduction Band
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Mobile Electrons
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Density Functional Theory
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Coumarin Dye
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Organic Dye
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Coumarin Dye
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Solar Equipment
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Photo Electrochemical cell
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Efficient Conversion
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Solar Energy
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Solar Equipment
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\n\t\t\t\t\t\t
Material Nanostructure
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Material Nanostructure
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Redox Reaction
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Electron Transport
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Electron Transpot
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Back Reaction
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ZnO
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ZnO
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Nanowire
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Nanorod
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Semiconducting zinc compounds
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Scanning Electron Microscopy
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Scanning Electron Microscopy
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X-ray Diffraction
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Transmission Electron Microscopy
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Electron Microscopy
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X-ray Diffraction Analysis
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X-ray Photoelectron spectroscopy
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\n\t\t\t\t\t\t
Open Circuit Voltage
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Open Circuit Voltage
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Fill Factor
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Electrochemical Impedance Spectroscopy
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Electrochemical Impedance Spectroscopy
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Electrochemical Corrosion
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Ion Exchange
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Nanotube
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Nanotube
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Anode
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Nanotube TiO2
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Table 6.
Stage 4 – Top Tier DSSC PCA Factors and Constituent Phrases
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\n\t\t
\n\t\t\t
5. Discussion
\n\t\t\t
Recent attention to themes like “Big Data” and “MoneyBall” draw attention to the potential in deriving usable intelligence from information resources. We have noted the potential for transformative gains, and some potential unintended consequences, of exploiting information resources [53]. Term clumping, as presented here, offers an important tool set to help move toward real improvements in identifying, tracking, and forecasting emerging technologies and their potential applications.
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Desirable features in such text analytics include:
\n\t\t\t
Transparency of actions – not black box
Evaluation opportunities – we see value in comparing routines on datasets to ascertain what works better; we recognize that no one sequence of operations will be ideal for all text analytics
\n\t\t\t
Phrase consolidation advantages stand out in one DSSC example. Starting with some 2000 terms relating to variations of titanium dioxide (e.g., TiO2, TiO2, TiO2 film), we reduce to 4 such terms, with the “combine term networks” (Step f) particularly helpful.
\n\t\t\t
We are pointing toward generation of a macro that would present the analyst with options as to which cleaning and clumping steps to run, in what order; however, we also hope to come up with a default routine that works well to consolidate topical terms and phrases for further analyses
\n\t\t\t
Some future research interests have been noted in conjunction with the list of steps, of which we are actively working on Steps h, j, and k. We are particularly interested in processing unigrams, because of the potential in such approaches to work with multiple languages. On the other hand, we appreciate the value of phrases to convey thematic structure. Possibilities include processing single words, through a sequence of steps to Topic Modeling, and then trying to associate related phrases to help capture the thrust of each topic.\n\t\t\tWe see potential use of clumped terms and phrases in various text analyses.To mention two relating to competitive technical intelligence (CTI) and Future-oriented Technology Analyses (FTA):
\n\t\t\t
Combining empirical with expert analyses is highly desirable in CTI and FTA – clumped phrases can be further screened to provide digestible input for expert review to point out key topics and technologies for further scrutiny
Clumped phrases and/or PCA factors can provide appropriate level content for Technology RoadMapping (TRM) – for instance, to be located on a temporal plot.
\n\t\t\t
We recognize considerable interplay among text content types as well.This poses various cleaning issues in conjunction with co-occurrence of topical terms with time periods, authors, organizations, and class codes.We look forward to exploring ways to use clumped terms and phrases to generate valuable CTI.
\n\t\t
\n\t\t\n\t\t
Key Acronyms:
\n\t\n\t\t\t
CTI - Competitive Technical Intelligence
DSSCs - Dye-Sensitized Solar Cells [one of two topical test sets]
LSI - Latent Semantic Indexing
MOT - Management of Technology [the second of two topical test sets]
NLP - Natural Language Processing
PCA - Principal Components Analysis
ST&I - Science, Technology & Innovation
TM - Topic Modeling
WoS - Web of Science (including Science Citation Index)
We acknowledge support from the US National Science Foundation (Award #1064146 – “Revealing Innovation Pathways: Hybrid Science Maps for Technology Assessment and Foresight”). The findings and observations contained in this paper are those of the authors and do not necessarily reflect the views of the National Science Foundation.
\n\t\t\t
We thank David J. Schoeneck for devising groundrules for a semi-automated, 3-tier PCA and Webb Myers for the macro to combine term networks.Nils Newman has contributed pivotal ideas as we build our term clumping capabilities and determine how to deploy them.
\n\t\t
\n\t\n',keywords:null,chapterPDFUrl:"https://cdn.intechopen.com/pdfs/37723.pdf",chapterXML:"https://mts.intechopen.com/source/xml/37723.xml",downloadPdfUrl:"/chapter/pdf-download/37723",previewPdfUrl:"/chapter/pdf-preview/37723",totalDownloads:4297,totalViews:457,totalCrossrefCites:7,totalDimensionsCites:9,totalAltmetricsMentions:1,introChapter:null,impactScore:3,impactScorePercentile:83,impactScoreQuartile:4,hasAltmetrics:1,dateSubmitted:"March 14th 2012",dateReviewed:"June 21st 2012",datePrePublished:null,datePublished:"November 21st 2012",dateFinished:"July 5th 2012",readingETA:"0",abstract:null,reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/37723",risUrl:"/chapter/ris/37723",book:{id:"2746",slug:"theory-and-applications-for-advanced-text-mining"},signatures:"Alan Porter and Yi Zhang",authors:[{id:"153097",title:"Prof.",name:"Alan",middleName:null,surname:"Porter",fullName:"Alan Porter",slug:"alan-porter",email:"alan.porter@isye.gatech.edu",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Georgia Institute of Technology",institutionURL:null,country:{name:"United States of America"}}},{id:"153196",title:"Mr.",name:"Yi",middleName:null,surname:"Zhang",fullName:"Yi Zhang",slug:"yi-zhang",email:"yi.zhang.bit@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Beijing Institute of Technology",institutionURL:null,country:{name:"China"}}}],sections:[{id:"sec_1",title:"1. Introduction: Concepts, Purposes, and Approaches",level:"1"},{id:"sec_2",title:"2. Review of Related Literatures",level:"1"},{id:"sec_3",title:"3. Empirical Investigation:Two Case Analyses",level:"1"},{id:"sec_4",title:"4. Term Clumping Case Results",level:"1"},{id:"sec_5",title:"5. Discussion",level:"1"},{id:"sec_6",title:"Key Acronyms:",level:"1"},{id:"sec_7",title:"6. 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Computers\n\t\t\t\t\t2005\n\t\t\t\t\t12\n\t\t\t\t\t4\n\t\t\t\t\t1794\n\t\t\t\t\t1801\n\t\t\t\t\n\t\t\t'},{id:"B47",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSayyadi\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHurst\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMaykov\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tEvent Detection and Story Tracking in Social Streams.\n\t\t\t\t\tProceeding of 3rd Int’l AAAI Conference on Weblogs and Social Media, ICWSM09, May 17-20, 2009, San Jose, California, USA; 2009\n\t\t\t\t\n\t\t\t'},{id:"B48",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKongthon\n\t\t\t\t\t\t\tA. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tText Mining Framework for Discovering Technological Intelligence to Support Science and Technology Management, Doctoral Dissertation,\n\t\t\t\t\tGeorgia Institute of Technology\n\t\t\t\t\t2004\n\t\t\t\t\thttp://smartech.gatech.edu/bitstream/handle/1853/5151/kongthon_alisa_200405_phd.pdf.txt?sequence=2\n\t\t\t\t\t(accessed 20 May 2012)\n\t\t\t\t\n\t\t\t'},{id:"B49",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKongthon\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHaruechaiyasak\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tThaiprayoon\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tConstructing Term Thesaurus using Text Association Rule Mining.\n\t\t\t\t\tProceedings of the 2008 Electrical Engineering/Electronics, Computer, Telecommunications and Information Technology International Conference, ECTI2008.\n\t\t\t\t\n\t\t\t'},{id:"B50",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKongthon\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t, \n\t\t\t\t\t\t\tAngkawattanawit\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tDeriving Tree-Structured Network Relations in Bibliographic Databases.\n\t\t\t\t\tProceedings of the 10th International Conference on Asian Digital Libraries, ICADL 2007, December 10-13, Hanoi, Vietnam; 2007\n\t\t\t\t\n\t\t\t'},{id:"B51",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCourseault-Trumbach\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPayne\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tIdentifying Synonymous Concepts in Preparation for Technology Mining\n\t\t\t\t\tJournal of Information Science\n\t\t\t\t\t33\n\t\t\t\t\t6\n\t\t\t\t\t2007\n\t\t\t\t\n\t\t\t'},{id:"B52",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWatts\n\t\t\t\t\t\t\tR.J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPorter\n\t\t\t\t\t\t\tA.L.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tR&D cluster quality measures and technology maturity.\n\t\t\t\t\tTechnological Forecasting and Social Change\n\t\t\t\t\t2003\n\t\t\t\t\t70\n\t\t\t\t\t8\n\t\t\t\t\t735\n\t\t\t\t\t758\n\t\t\t\t\n\t\t\t'},{id:"B53",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPorter\n\t\t\t\t\t\t\tA.L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRead\n\t\t\t\t\t\t\tW.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tThe Information Revolution: Current and Future Consequences.\n\t\t\t\t\tWestport, CT: JAI/Ablex; 1998\n\t\t\t\t\n\t\t\t'},{id:"B54",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZhang\n\t\t\t\t\t\t\tY.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPorter\n\t\t\t\t\t\t\tA. L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHu\n\t\t\t\t\t\t\tZ.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tAn Inductive Method for “Term Clumping”: A Case Study on Dye-Sensitized Solar Cells.\n\t\t\t\t\tThe International Conference on Innovative Methods for Innovation Management and Policy, IM2012, 23-26May 2012, Beijing, China; 2012\n\t\t\t\t\n\t\t\t'},{id:"B55",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPorter\n\t\t\t\t\t\t\tA.L.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchoeneck\n\t\t\t\t\t\t\tD.J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tAnderson\n\t\t\t\t\t\t\tT.R.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\tPICMET Empirically: Tracking 14 Management of Technology Topics.\n\t\t\t\t\tPortland International Conference on Management of Engineering and Technology, PICMET2012, 29 July-2 August, Vancouver, Canada\n\t\t\t\t\t2012\n\t\t\t\t\n\t\t\t'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Alan L. Porter",address:"alan.porter@isye.gatech.edu",affiliation:'
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1. Introduction
Soybean (Glycine max (L.) Merrill.) has a central position in agriculture along with barley, cassava, groundnut, maize, millet, potato, oil palm, rapeseed, rice, rye, sorghum, sugar beet, sugarcane, sunflower, and wheat which were considered as the most cultivated plants worldwide. Its central role is not only constituted due to the dense protein and high-quality oil contents but also industrial raw material supply. Tofu, soy milk, soy sauce, and miso are the main nutritious human soy products. Also, extracted soy oil, with over 75% oleic acid and under 10% polyunsaturated fatty acids, is one of the most preferred oils sold commercially in the United States today [1]. Long shelf-life required fry, spray, and ingredient oils should preferably contain higher oleic acid due to the better persistence to oxidation. Soy meal is also a major source of protein used in pig and poultry industries. The companion animal industry prefers soy meal as a protein source in animal diet, especially for dogs. High-quality amino acid composition and highly digestible protein content leads to the use of soy meal in aquaculture diets [2]. On the other hand, soy oil has various industrial uses as pharmaceuticals, plastics, papers, inks, paints, varnishes, and cosmetics.
In the verge of global warming effects, renewable energy sources as an alternative to fossil fuel are getting importance. Soybean is also an important biodiesel crop in many countries along with maize, especially in South America countries [3]. Besides the alternative bioenergy crop role, it has also environmental effects as being capable of utilizing atmospheric nitrogen through biological nitrogen fixation and is therefore less dependent on synthetic nitrogen fertilizers. While drought is one of the most plant growth and development limiting factors in present days, nitrogen deficiency is equally crippling for plants, as well, due to its structural, genetic, and metabolic functions in crop yield. Highly stable and non-reactive N2 is the most abundant constituent of the Earth’s atmosphere, still no eukaryotic organism can use it directly. Some members of Leguminosae (Fabaceae) family including soybean have adopted the ability to establish symbiotic interactions with diazotrophic bacteria known as rhizobia in evolutionary adaptations. By this means, a process called ‘biological nitrogen fixation is a low-cost N source that sufficiently increases soybean yield with low environmental impact and avoids the use of synthetic N fertilizers [4].
Soybean (G. max (L.) Merr) as a member of the family Fabaceae/Leguminosae, subfamily Papilionoideae, and the tribe Phaseoleae contains two subgenera as Glycine which has 26 perennial species and Soja (Moench) F.J. Herm. having four annual species [5]. Domestication of cultivated soybean can be traced back to China in 5000 years ago, however, the geographical origin of Glycine genus can be traced back to putative ancestor (2n = 2x = 20) which was presumably migrated and formed unknown or extinct wild perennials (2n = 4x = 40) in China. Wild annuals (2n = 4x = 40; Glycine soja) and domesticated soybean (2n = 4x = 40; G. max) subsequently evolved [6]. The genetic diversity of G. max is assumed to regress due to man-made genetic bottlenecks through selection for high yielding lines in modern plant breeding applications. Indeed, yield is the backbone of the profitability and the feasibility. Varieties with other superior traits are not significant in industrial scale unless they have a high yield. As well as yield, maturity, herbicide, and pest resistance, lodging resistance, shattering resistance, seed quality and composition, abiotic stress tolerance are other breeding selection targets [7]. While the wild relative G. soja grows in various environmental conditions and have not been exposed to the selective bottlenecks, it retained significant genetic diversity over time.
On the other hand, soybean flowers represent cleistogamous characteristics. Cleistogamy, which is described as the production of both open (chasmogamous, CH) and closed (cleistogamous, CL) floral forms by one species, is very common among angiosperms. Soybean is pseudocleistogamous cleistogamy in which no morphological differences between CL and CH flowers occur other than a lack of expansion of petals and anthesis in CL flowers. It may also be induced by environmental stress factors, occasionally. Cleistogamy is observed both in cultivated soybean [G. max (L.) Merr.] and its wild relative [G. soja Sieb. & Zucc.]. Soybean usually produces both CH and CL flowers on the same plant. In these plants, fertilization occurs within closed petals of CL flowers [8, 9, 10]. The rates of natural cross-pollination have been observed between ranges of 0.03–1.14% in natural conditions for self-pollinating soybean plant [11]. Thus, cleistogamy may have influenced the genomic homogeneity and reduced genomic variation further in soybean along with domestication practices.
In this context, improving genomic variations is crucial in soybean breeding. This chapter will summarize present conventional and biotechnological methods in soybean breeding and emphasize on mutation breeding practices with the concluding discussion on future prospective.
2. Improving genomic variations
2.1 Conventional methods
In soybean breeding, oil and protein content, resistance to biotic and abiotic stresses have been the main breeding objectives in past decades. In conventional breeding practices variability of desired traits is based on the detection of novel genotypes which contains enhanced characteristic for the trait. Hybridization of these novel genotypes with the varieties which are already in use for commercial production is the base of the process. Subsequent, the selfing of progenies, which contain traits distributed according to basic genetical segregation rules, provide novel genotypes. Detection of the most favorable recombination in those progenies which is also referred as homozygosity by selection is based on numerous selection methods including pedigree selection, single-seed descent, bulk breeding, mass selection, selection among half-sib families, selection within half-sib families. However, the traditional pedigree method and the single-seed descent method (SSD) are the most successful and preferred in soybean breeding. The last step in the process is yield testing. Available genotypes and technical infrastructure (agricultural machines, greenhouses, and experienced stuff) as well as breeding objective are deciding factors in method selection. Breeding objectives generally depend on the local agroecological conditions, available acreage, production intensity, market demand, and economical value [11, 12, 13].
Pedigree selection is a highly labor-intensive method that depends on visual selection by the appearance in each generation. In this method, desirable genotypes are selected in each generation and the limited number of selected genotypes are advanced to the next generation by inbreeding/selfing. The labor intensity of the method is limiting for large scale breeding practices [14]. Single-seed descent (SSD) is the most preferred method with pedigree selection to increase homozygosity in soybean. Single pod descent (SPD) accelerates the SSD for harvesting process even further. This method is mostly preferred for high seed yield, oil content and quality, resistance to biotic and abiotic stresses and maturity duration breeding objectives [15].
2.2 Biotechnological approaches
Although, the improvement of plants by conventional breeding methods is one of the most preferred breeding strategies, the limited hybridization among species, transfer of undesirable genomic segments together with genes of interest (e.g., linkage drag) and the fact that diversity in species is based on spontaneous mutations with a very low frequency necessitated the development of new breeding strategies. Plant breeding has often benefited from new technologies to overcome such limitations. Molecular breeding as one of these strategies can be extensively defined as the utilization of genetic manipulation of DNA at the molecular level to improve of trait of interest in plants, including genetic engineering, molecular marker-assisted selection, marker-assisted backcrossing, marker-assisted recurrent selection, genome wide selection [16, 17]. Molecular breeding requires more complex equipment and molecular tools compared to conventional breeding approaches. The identification of functional genes and DNA markers associated with variation at the genomic level is an important part of molecular breeding. Marker-assisted breeding (MAB) which utilized marker-assisted selection involves the use of molecular markers in conjunction with linkage maps and genomics, and the improvement of crop plant traits based on genotypic analyses. Moreover, MAB requires minimum phenotypic information during the training phase. The convenience of use and analysis, low cost, a small amount of DNA requirement, co-dominance, reproducibility, high-rate polymorphism and genome-wide distribution are the most important factors for molecular tools used in marker-assisted breeding (MAB) in plants [18]. Along with the emergence of marker-assisted selection (MAS) after the mid-1980s, rapid improvement of plant yield and quality has been achieved thanks to the development of molecular maps by utilizing structural and functional genomics in plant breeding. MAS can be classified into five broad areas: marker-assisted evaluation of breeding material; marker-assisted backcrossing; marker-assisted pyramiding; early generation selection and combined MAS [19].
DNA markers have made significant contributions to increasing the efficiency of conventional and mutation breeding through marker-assisted selection and have been integrated into traditional schemes to develop novel varieties or used instead of traditional phenotypic selection. Many DNA marker techniques have been developed based on different polymorphism detection techniques or methods (such as nucleic acid hybridization, restriction enzyme digestion, PCR, DNA sequencing) such as RFLP, AFLP, RAPD, SSR, SNP. Advances in molecular marker techniques and the creation of large-scale marker datasets provide a reliable way to identify and trace the genetic basis of important agricultural traits. Molecular markers developed from functional genes have been used for the development of soybean varieties by improving important agricultural traits such as yield, disease resistance and abiotic stress tolerance [20]. Breeders can combine all the suitable alleles in a single variety to develop desired crops, thanks to molecular markers closely related to particular traits. However, although soybean yield remains the most important selection criterion for soybean breeders and the primary factor for profitability, it is very difficult to acquire complex traits such as yield, quality and abiotic stresses with marker-assisted selection. Genomic selection (GS) is a promising approach that leverages molecular genetic markers to design new breeding programs and develop new marker-based models for genetic evaluation. GS, which has high selection accuracy, reduced selection duration, greater gain per unit time, precise and accurate results provide breeders with opportunity faster development of improved crop varieties for complex traits. New marker technologies, such as NGS-based genotyping, have made the use of genomic selection as routine for crop improvement while increasing the efficiency of marker applications. The availability of genome-wide high-throughput, low-cost and flexible markers, usability for crop species with or without a reference genome sequence with a large population size are the most important factors for its successful and effective implementation in crop species [21].
Plant breeders have begun to take advantage of molecular breeding more through advances in the identification of QTLs/genes responsible for important agronomic traits. Numerous quantitative character loci (QTL) mapping studies performed for a variety of agricultural crops have resulted in the association of DNA markers and traits. The most notable high-throughput genotyping system is single-nucleotide polymorphisms (SNPs), which are heavily used in quantitative character locus (QTL) discovery. More than 10,000 QTLs using different marker systems have been reported in more than 120 studies involved 12 plant species aimed at improving quantitative properties with economic importance [22]. Linkage analysis for QTL mapping is frequently preferred in two-parent populations. Genotyping by next-generation sequencing become prominent as a promising technology and is also used for genome-wide association studies (GWAS) to identify useful genes to increase crop productivity. Soybean genome sequence information, as one of the most substantial resources, is the basis of genomic studies and has allowed the significant development of genomic applications for soybean breeding.
As in transgenesis, studies involving the transfer of a limited number of loci from one genetic background to another are also within the scope of molecular breeding. Especially in the last two decades, genetic engineering approaches that generate novel genetic variations in plant genome or enable the transfer of gene of interest for obtaining original traits to plants have been frequently preferred among the biotechnological approaches that have been successfully applied in plant breeding [23, 24, 25]. Along with recent developments in recombinant DNA technology, it has been paved the way for transferring the desired characteristics to plants within plant breeding in a short time. These genetic engineering and plant transformation approaches which make plant breeding faster, more predictable and improvable for a wide variety of species, include successful characterization, cloning, modification and transfer of DNA expressed the desired trait into cells. The gene pool utilized by plant breeders in conventional breeding since the mid-1990s has been considerably expanded by genetic transformation approaches and many different transgenic plants have been developed by transferring traits that are tough to transfer [26, 27, 28]. Genetically Modified Organisms (GMOs), whose agricultural traits have been improved through inter-species gene transfer by utilizing genetic engineering techniques, have been increasingly planted, globally. The total cultivation areas of approved GM plants have increased approximately 113 times, from 1.7 million hectares in 1996 to 191.7 million hectares in 2018. This increase reveals that transgenic technology is the fastest adopted technology in recent years. A total of 2.5 billion hectares or 6.3 billion acres GM crops have been planted in the first 23 years (1996–2018) of commercialization of transgenic plants [29]. Especially soybean (95.9 million hectares) which comprises 50% of the global area of GM crops, corn (58.9 million hectares), cotton (24.9 million hectares) and canola (10.1 million hectares) are the four main transgenic crops cultivated. Transgenic crops, which were initially developed for only producers/farmers on the purpose of agriculture such as insect resistance and herbicide tolerance, afterwards were developed for other traits such as disease resistance, abiotic stress tolerance, modified product quality for both the producers/farmers and consumers. Especially cultivation of stacked events which are GM crops with more than one genetic modification, gather momentum.
During the 23-year period from 1996 to 2018, herbicide tolerance has accounted for the majority of transgenic crops area planted. Only herbicide tolerance cultivation areas of transgenic crops have been gradually decreasing over the years with the increasing importance of stacked cultivars with multiple traits (e.g., both insect resistance and herbicide tolerance; IR/HT). In 2018, stacked (IR/HT) traits used in soybean, maize and cotton have accounted for 42% of the total transgenic acreage, up 4% annually. Traits such as herbicide tolerance, insect resistance, disease resistance, pollination control, modified crop quality, anti-allergy, delayed fruit softening, delayed ripening, enhancement of vitamin A content, modified alpha-amylase, modified amino acid, modified oil/fatty acid, modified starch/carbohydrate, nicotine reduction, non-browning phenotype, phytase production, reduced acrylamide potential, reduced black spot bruising have been transferred to plants and many of these have been combined in various combinations [29]. Thanks to these features brought to agricultural plants, the product yield obtained from the cultivation areas increases significantly. Along with the acceleration of the transfer of the appropriate gene combinations to plants with high added value, products that can provide significant gains in the agricultural economy have been developed. In this process, about 30 different types of transgenic plants such as particularly G. max (soybean), Zea mays (corn), Gossypium hirsutum (cotton), Bassica napus (canola) and including fruits and vegetables such as Phaseolus vulgaris (bean), Prunus domestica (plum), Beta vulgaris (sugar beet), Solanum melongen (eggplant), Cucumis melo (melon), Carica papaya (papaya) have been approved [30]. Stacked traits such as Intacta™Roundup Ready™ 2 Pro, Enlist E3™ and Vistive Gold™ soybeans are favored by farmers for their cost-saving technologies. In 2018, the planting of crops with novel stacked traits in various combinations, including herbicide-tolerant and high-oleic acid soybean, herbicide-tolerant and salt-tolerant soybean varieties were approved. The global acreage of soybeans in 2018 was 123.5 million hectares, of which 78% (95.9 million hectares) were GM soybeans. GM soybeans have been planted on 95.9 million hectares, 50% of the global cultivated area for GM crops; USA (34.1 million hectares), Brazil (34.9 million hectares), Argentina (18.0 million hectares), Paraguay (3.35 million hectares), Canada (2.42 million hectares), Uruguay (1.26 million hectares), Bolivia (1.26 million hectares) and Southern Africa (694,000 hectares). In the USA, soybean is the second most important crop with a total cultivated area of 36.26 million hectares in 2018, with 94% GM. These GM soybeans contain herbicide-tolerant traits that control a variety of weed species depending on the genes deployed. Other features incorporated into HT soybeans include consumer properties such as high monounsaturated oleic acid and enriched omega-fatty acid. In Brazil which has the second-largest GM crop cultivation area with 51.3 million hectares in 2018, GM soybean was planted in an area of 34.86 million hectares. As for Argentina which was the third country to plant the most GM crops in 2018, 18 million hectares of soybeans were planted [29, 30].
2.3 Mutation breeding
Term of mutation was first introduced by de Vries as the sudden and unexpected emergence of hereditary alterations in defining traits apart from recombination in Mutation Theory Vol. I [31]. In 1920s, following Stadler’s experiments on genetic effects of X-rays on maize, plant breeders started to use physical and chemical mutagens to induce heritable mutations in plants [32]. As a term, mutation breeding is introduced to the scientific world by Freisleben and Lein defined as the deliberate exposure of biological materials to mutagens for induction of mutation frequency exceeding the natural mutation frequency to develop new varieties [33].
Mutations that cause genetic variation among living organisms can be categorized under spontaneous and induced mutation terms. Spontaneous mutations, which occur in low frequency and accumulate for a long time, allow plants to adapt very distinct environments apart from their original habitat [34, 35, 36].
The spontaneous mutation may occur due to the exposure to physical (cosmic radiation, natural background radiation of earth), chemical (alkylating agents, base analogs, antibiotics) mutagens and biological factors (transposon activation) during the reproductive stage. Spontaneous mutation frequency is calculated as 10−6 in plants during DNA replication, repair, or genomic element activities [37]. In vitro and in vivo propagation processes may also trigger gene methylation and cause epigenetic alterations while transposon mobility may trigger somoclonal variation and increase spontaneous mutations. Loss or activation of gene through transposable elements (TEs) regulate many biological processes. There are various studies on somoclonal variation-based trait improvement in plants. However, low mutation frequency is a real draw back for considering this method as common breeding alternative [35, 37]. Mutations can also be induced through physical and chemical mutagens. The use of mutagens may induce 103-fold more mutants comparing to the spontaneous mutations. Ossowski et al. [38] calculated spontaneous mutation frequency as 7x10−9 substitutions per site per generation for Arabidospsis plant in 30 generations. This frequency was increased by ethyl methanesulfonate (EMS) treatment to 3x10−5 substitutions per site per generation. EMS is a mutagenic, teratogenic, and carcinogenic organic compound with formula C3H8SO3 which produces random mutations, mostly G:C to A:T transitions induced by guanine alkylation, in genetic material by nucleotide substitution. EMS typically produces only point mutations. Genetic alterations due to physical and chemical mutagens can be classified as genome, chromosome, and gene mutations [31, 35, 39, 40, 41, 42].
Genome mutations not only affect genome size (ploidy) but also genome re-arrangement in plants. Many plant species as bread wheat: 6X; durum wheat: 4X, cotton: 4X, potato: 4X have polyploidy in nature. Polyploidy leads various advantages as enhanced nucleus size, enlargement on cell and organism basis, yield, increase in gene variations. Polyploidy can be induced as genome duplication (autoploidy) and increase in genome size (alloploidy) through use of mutagens [34, 43].
Chromosome mutations occur during meiotic cell division in very low frequencies. In euploidy state of plants, one set of chromosomes are present, while radiation exposure may result whole or partial chromosome deletions, insertions or translocations and cause aneuploidy. Besides, chromosome inversions, which are characterized as a chromosome rearrangement in which a segment of a chromosome is reversed 180 degrees end-to-end, cause very high gene recombination. In chromosome translocations, break off chromosome parts may attach to the same chromosome (intra-chromosomal) or different chromosome (inter-chromosomal). Both, inter- and intra-chromosomal translocations lead to devastating effects on gene expression.
Gene mutations can be either as gene copy number alterations or as point-mutations, insertions, deletions on nucleotides of gene sequence. Plants may increase gene copy numbers to enhance protein expression during metabolic functions. Mutagens can affect gene expression profiles through either by increasing or decreasing gene copy numbers. Point-mutations occur particularly in chemical mutagen applications. Single or set of nucleoid alterations cause silent mutations if they do not occur in genic regions. Alternatively, they can also cause nonfunctional gene products or nonsense mutations. Nucleotide insertions or deletions can alter codon structure and cause shift in open reading frames. These alterations can also occur on promotor regions, coding sequences or intron regions of genes, therefore, significantly effect protein expression [34, 35].
Single nucleotide changes as deletions generally cause functional gene mutations by the leading formation of novel alleles. Hence, they are particularly important for plant breeding studies for inducing genetic variations. There are numerous examples of plant height, abiotic stress tolerance, pesticide and herbicide resistance improvement cases in rice, wheat, barley, soybean plants, and more [44, 45, 46, 47]. Nucleotide mutations can also occur in non-genic regions and cause silent mutations which have no apparent effect on gene expression. Silent mutations generally occur following the alkylating chemical applications and do not affect translation [37]. Deletions among intergenic regions remain silent as long as they do not affect regulating sequences. Still, the possibility of open reading frame shift is present and may lead to nonfunctioning peptide formation [35].
In mutation breeding studies, whole plant, meristem tips, pollens, in vitro explants, embryos, microspores, callus cultures can be selected as initial materials. However, seeds are mostly preferred materials by plant breeders due to the advantages as metabolic inactivity, easy transport, ease of application, low space requirement, ease of storage comparing to others.
2.3.1 Mutagens
Choice of appropriate mutagen is one of the deciding factors on succession of the mutation breeding program. Physical, chemical, or biological agents are viable alternatives. Among physical mutagens ionizing radiation sources, particle (electrons, protons, neutrons, alpha and beta particles) or electromagnetic (X-rays, gamma rays), are widely used. Ionizing radiation interacts with genetic material and cause mutations on DNA sequences. Magnitude of mutagenic effect is proportional to the radiation dose. It is crucial to determine and optimize the effective radiation dose based on experimental plant variety, plant part, and radiation source. 80% of mutation breeding studies prefer physical mutagens and of 60% of this use gamma radiation [35].
Chemical mutagens offer much larger alternative choices. However, the most widespread use of chemical mutagens is among alkylating agents. Ethyl methane sulphonate (EMS), diethyl sulphate (DES), ethylene imine (EI), N-ethyl-N-nitroso urea (ENU), ethyl nitrite urethane (ENU), N-methyl-N-nitrosourea (MNU) are the most generally preferred chemicals. O6-alkylguanine, N3-alkyladenine, N3-alkylcitosine leads to alternative allele formation. Besides methylating agents, nitric acid, nucleic acid analogs, some antibiotics (streptozotocin, mitomycin C, azaserine) are other important chemical mutagens. 60% of registered chemically induced mutant plants are developed by use of EMS, MNU and EMU. One-third of these mutants are obtained by EMU which has ease of supply among others.
Among physical mutagens, gamma radiation has the most frequent use. In nature, there are various gamma-emitting isotopes such as potassium-40 (40K), however, in plant breeding applications cobalt-60 (60Co) and cesium-137 (137Cs) are the common choices.
In the last 20 years, there are 599 different developed mutant plants belonging to 78 different plant species registered to International Atomic Energy Agency (IAEA) Mutant Variety Database [48]. Soybean is in the third place among these plants with 46 registered mutants (8%) after 247 rice (42%) and 55 wheat (9%) mutants. In the category referred as others, chickpea, carnation, tomato, mung bean, Hibiscus, rapeseed, sesame, orchid, pepper, cowpea, glory bush and sunflower have the most mutants (Figure 1).
Figure 1.
Mutation variety database of IAEA registered mutant plants in last 20 years [48].
Among the soybean mutants, there are 15 different improved traits. They can be listed as; high yield, high protein content, resistance to soybean mosaic virus (SMV), early maturity, resistance to leaf rust, resistance to purple seed stain, resistance to cyst nematode (SCN), resistance to lodging, drought tolerance, super nodulation, absence of lipoxygenase, temperature tolerance, low allergenicity and higher nitrogen fixation (Figure 2). Thirty-six of these traits were improved by the use of gamma rays as physical mutagens, while 7 of them were developed by chemical mutagens. In this period, China is the leading country with 9 registered soybean mutants while Japan (9), Viet Nam (5), Bulgaria (3), India (3), Indonesia (3), Republic of Moldova (3)Republic of Korea (1) and Thailand (1) are the followers.
Figure 2.
Radiation-induced trait improvements achieved and registered to MVD in last 20 years.
2.3.2 Present applications of mutation breeding in soybean
In the last decades of mutation breeding, radiosensitivity of different plant species and tissues were investigated and dose limits were determined for various plants. In present days, molecular marker-based techniques were widely applied to estimate genetic diversity and population structure. Among these techniques restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), and inter-simple sequence repeats (ISSRs) are viable options depending on the advantages and limitations of each technique. SNPs, which are spread across in both non-coding and coding regions of the genome, are also preferred in many mutation studies [49]. Present applications of the marker-based techniques include even transposable elements (TEs). The target region amplification polymorphism (TRAP) is a novel, polymerase chain reaction (PCR)-based marker system which exploits the available EST database sequence data to generate polymorphic markers targeting candidate genes. This method utilizes an 18-mer primer derived from the EST sequence and pairs it with an arbitrary primer that targets the intron and/or exon region. TRAP method is useful for germplasm genotyping and producing markers associated with desirable agronomic traits in mutation breeding. Hung et al. [50] employed this simple rapid method by using the consensus terminal inverted repeat sequences of PONG, miniature inverted-repeat transposable element (MITE)-Tourist (M-t) and MITE-Stowaway (M-s) as target region amplification polymorphism (TE-TRAP) markers to investigate the mobility of TEs in a gamma-irradiated soybean mutant pool. They concluded that MITEs were significant enough to confirm their practical utility as molecular markers for investigating mutant populations which were induced by random variations caused through physical mutagenesis (X-ray or gamma-ray). Also, the TE-TRAP marker system was suggested as it provides a simple, rapid, and cost-effective alternative for investigating genetic diversity and identifying mutant lines in irradiated soybean mutant breeding. Kim et al. [51] conducted a genetic diversity and association analysis of soybean mutants to assess elite mutant lines which were induced by 250 Gy of gamma rays using a 60Co gamma-irradiator. They have chosen 208 soybean mutants by phenotypic traits to mutant diversity pool (MDP) and investigated the genetic diversity and inter-relationships of these MDP lines using TRAP markers. MDP has been suggested to have great potential for soybean genetic resources. TRAP markers were found useful for the selection of soybean mutants in mutation breeding applications [51].
Besides the genetic diversity and population structure analysis, genetic characterization of improved mutants and the determination of the source of the gained trait in sequence basis studies have taken over the course of mutation breeding in present days. Before the genomic era which was ignited through the breakthrough discovery of DNA sequencing by Sanger et al. [52], the heteroduplex mismatch cleavage assay which is based on mismatch-specific endonuclease Cel I, was the standard method to detect point mutations. As a simple, rapid, and cheaper mutant discovery method, high resolution melting (HRM) analysis was applied to many agronomical crops. Following the Sanger sequencing, the final step of mutation screening was changed to Sanger to evaluate the changes in the genome and effects of mutation on amino acid substitutions. Today, next generation sequencing (NGS) technologies are the gold standard in the mutation detection field with various options as Roche 454 pyrosequencing, sequencing-by-synthesis, SOLiD sequencing and the HiSeq 2000, which is the gold standard of high-throughput sequencing. Tsuda et al. [53] reported the construction of a high-density mutant library in soybean and the development of a mutant retrieval method referred as amplicon sequencing which is an alternative, cheaper method for sequencing the PCR amplicons in targeted regions. The library of DNA and seeds of EMS-induced plants revealed large morphological and physiological variations. They retrieved the mutants through HRM and indexed amplicon sequencing analysis and confirmed by Sanger sequencing in the final step. They concluded that indexed amplicon sequencing allows researchers to scan a longer sequence range and skip screening steps and also, to know the sequence information of mutation due to the utilization of systematic DNA pooling and the index of NGS reads, which simplifies the discovery of mutants with amino acid substitutions comparing to the HRM screening [53].
MutMap method which utilizes the sequencing technique for mapping the mutated genes responsible for the desired trait was introduced for mutation breeding studies. The first application of the method has been developed by Abe et al. [54] to identify the mutated gene responsible for the change in leaf color from dark green to light green in rice [54]. Thereafter, it has been commonly used for mapping the monogenic recessive genes. In this method, a cultivar with a known reference sequence can be mutagenized by either chemical or physical mutagens. After the selfing and homozygosity experiment for the desired trait between M3 to M6 generations, mutants are crossed with their parental or wild type varieties. F2 population is obtained by selfing of F1. If the desired trait is inherited through a single recessive gene, the segregation ratio should be of 3:1 in wild and mutant phenotype in F2 population. In MutMap method, DNA of homozygous mutant plants are extracted and subjected to whole genome sequencing. The mutant genomes are compared to the publicly accessible reference sequences to determine single nucleotide polymorphic (SNPs) variations. The linkage between mutants and wild type plants can be evaluated according to SNP ratios in which the ratio infer that the SNP variation is not linked to the mutation if ranged between 0.1 and 0.5, while it can be linked to the mutation when ranges are between 0.51 to 1 [54, 55]. Kato et al. [56] introduced Lumi-Map, which is a high-throughput platform for identifying causative SNPs for studying pathogen-associated molecular patterns (PAMP) triggered immunity (PTI) signaling components, in combination with MutMap. In Lumi-Map method, they generated nine transgenic Arabidopsis reporter lines expressing the LUC gene fused to multiple promoter sequences of defense-related genes, that generates luminescence upon activation of FLAGELLIN-SENSING 2 (FLS2) by flg22, a PAMP derived from bacterial flagellin treatment. Mutagenesis of the line as achieved through EMS treatment and the mutants with altered luminescence patterns were screened by a high-throughput real-time bioluminescence monitoring system. They subjected MutMap method on selected mutants to identify the causative SNP responsible for the luminescence pattern alterations. WRKY29-promoter reporter line was selected to identify mutants in the signaling pathway downstream of FLS2. Twenty-two mutants with altered WRKY29 expression upon flg22 treatment among 24,000 EMS-induced mutants of the reporter line were isolated. In this mutagenesis study, Lumi-Map method combined with MutMap revealed three genes not previously associated with PTI and suggested as a potential alternative to identify novel PAMPs and their receptors as well as signaling components downstream of the receptors [56]. Takagi et al. [57] exploited the rapid and versatile properties of MutMap for more than 20,000 ha of rice paddy field which was inundated with seawater, resulting in salt contamination of the land in Japan following the 2011 earthquake and tsunami that affected Japan. They needed an improved rice variety at short notice as local rice landraces were not tolerant of high salt concentrations caused by seawater. They obtained 6000 EMS-induced mutant lines of a local elite cultivar, ‘Hitomebore’. MutMap method was used to rapidly identify a loss-of-function mutation responsible for the salt tolerance of hst1 rice. The detected salt-tolerant hst1 mutant was used to breed a salt-tolerant Kaijin variety which differs from Hitomebore by only 201 SNPs. Conducted field trials presented that improved variety had the equal growth and yield performance as the parental line under normal growth conditions. The whole process was completed only in 2 years which proves the efficiency of MutMap in mutation breeding studies [57]. Fekih et al. [58] improved the method even further and introduced the MutMap+ which is a modified version of MutMap developed for the cases in which obtaining F2 mapping population is impossible due to the lethal mutations or sterility. MutMap+ has advantages over MutMap as it is less complex, time-consuming, and costly especially in large mapping population. Also, hybridization step of MutMap can be relatively compelling especially in small flower plant species and in crops that are recalcitrant to artificial crosses, therefore, MutMap+, which notably does not necessitate artificial crossing between mutants and the wild-type parental line, is advantageous. In MutMap+ method, again, a cultivar with known reference sequence can be mutagenized by either chemical or physical mutagens. M1 plants are selfed to develop M2 generation. However, in MutMap+ mutants are not crossed with their parental or wild type varieties. The heterozygous M2 mutant plants are selfed to develop M3 generation in which the segregation ratio of 3:1 for wild and mutant phenotype is expected. DNAs of tagged mutants and parental varieties are extracted, and pooled. Following the whole genome sequencing, data is compared to the reference genome and SNP profiles are determined. They identified causal nucleotide changes of rice mutants of NAP6 gene that is responsible for change in leaf color and consequent lethality after germination. This versatile extension of MutMap method, also allow determination of recessive lethal alleles [58].
In soybean, Liu et al. [59] investigated two types of resistant sources which are widely used against soybean cyst nematode (SCN, Heterodera glycines Ichinohe). Peking-type soybean requires both rhg1-a and Rhg4 alleles, while PI 88788-type soybean requires only the rhg1-b allele for resistance. Instead of MutMap, they preferred the region-specific extraction sequencing (RSE-Seq) method which is developed to enrich a targeted chromosomal segment for genome sequencing to identify SCN resistance genes within the identified 300 kb chromosomal segment carrying the rhg1 locus, due to the requirement of MutMap to an additional procedure of backcross of phenotypic mutants with the wild-type. They suggested GmSNAP18 gene as a candidate for the resistance of two various resistant types of soybeans for SCN [59]. RSE is a cost-effective, long-range DNA target capture methodology that relies on the specific hybridization of short (20–25 base) oligonucleotide primers to selected sequence motifs within the DNA target region This target enrichment method can produce sequencing templates more than 20 kbp in length. These capture primers are then enzymatically extended on the 3′-end, incorporating biotinylated nucleotides into DNA. Streptavidin-coated beads are subsequently used to pull down the original, long DNA template molecules through synthesized, biotinylated DNA that is bound to them [60]. QTL-seq is another method adapted from MutMap to identify quantitative trait loci. In presence of pooled two segregating progeny populations with opposite traits as resistant and susceptible and single whole-genome resequencing of either of the parental cultivars, it utilizes pooled sequences. Also, modified QTL-seq using high-resolution mapping has been developed to cover the weakness of original QTL-seq which do not assume a highly heterozygous genome [61]. Direct whole genome re-sequencing (WGRS) is also utilized effectively to identify candidate genes involved in resistance to SCN in soybean due to the requirement of time-consuming backcrosses in MutMap and QTL-seq methods. Two EMS-induced soybean mutants and six relevant whole genomes were re-sequenced to determine genomic variants as SNPs and InDels. Comparison by this method eliminated many genomic variants from the mutant lines that overlapped non-phenotypic but mutant progeny plants. Therefore, the method was suggested as simple but effective to the identify other trait genes in soybean, even in other organisms [62]. Likewise, comparative genomic analyses of two segregating soybean mutants which were selected among 500 EMS-induced candidates revealed seven genes potentially involved in resistance to Fusarium equiseti through WGRS. These genes were suggested to facilitate the breeding of resistant germplasm resources and the identification of resistance to Fusarium spp. in soybean [63].
3. Future Prospect and conclusion
Soybean genetic variation improvement is important for the development of superior cultivars. One of the greatest challenges in mutation breeding is random (uncontrolled) nature of induced mutagenesis. Large population requirement for desired mutant selection brings intensive labor. The emergence of clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology has brought wider insight to the field through allowing targeted mutagenesis. It has been widely used in numerous plants as rice, wheat, maize, oilseed rape, barley, cotton, tomato and soybean as well. However, utilization of CRISPR/Cas9 system in soybean is still limited due to the transformation challenges in soybean. As summarized in Table 1, most of the targets which were successfully applied to soybean were single gene edits. Paleopolyploid genome of soybean in which approximately 75% of the genes have multiple copies, requires multiple genes or paralogous genes to regulate many important traits. Therefore, these traits may only be targeted by editing which requires the engineering of homologous sequences using more than one sgRNA for recognition. Introducing multiple constructs simultaneously to soybean is relatively limiting in terms of genome editing associated soybean breeding approaches. Recently, Zhang et al. [73] successfully optimized one sgRNA CRISPR/Cas9 system in soybean for the target-specific mutations at multiple loci of GmFAD2 and GmALS. They evaluated the efficiency, type, specificity, and patterns of multiple targeted mutations by selecting three different genes with known functions in soybean and suggested that CRISPR/Cas9 could specifically and efficiently induce targeted mutations at one locus or multiple loci in the T0 generation. Moreover, they demonstrated the necessity of simultaneous modification of different homoeologous gene copies in polyploid soybean for successful CRISPR-Cas9-mediated breeding [73]. Therefore, induced mutagenesis is still a major method to produce new alleles and new desired traits within the crop genomes. Physical and chemical mutagen protocols are still improving and mutation breeding proves its value to be fast, flexible, and viable in crop sciences.
Targeted mutagenesis application examples for soybean.
The second most limiting prospect of induced mutagenesis was the requirement of at least three generation before any stable selection of desired traits in mutants which leads to 7−9 years of average mutation breeding study, previously. However, as described in previous sections NGS based approaches as MutMap accelerated the selection periods significantly. Novel non-destructive measurement methods allow automated imaging and optical measurements of the same plants for desired periods. These approaches provide high measurement densities and fill the gap between genotype and phenotype in mutation breeding studies which is still another limitation in this field. Repeated imaging of particular genotypes under different environmental conditions leads to the generation of development models for biologically relevant parameters. In the present omics era, future procedures may shorten the selection procedures even further [79].
In conclusion, mutation breeding passed important cross-roads successfully during recent advances in plant biotechnology, transformation and targeted mutagenesis by its particular great advantages. Mutagenesis will retain its place in crop science in next decades especially for the plants as soybean for which cross breeding is limited or not applicable.
\n',keywords:"soybean, mutation breeding, mutagens, induced mutagenesis, next generation sequencing",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/81525.pdf",chapterXML:"https://mts.intechopen.com/source/xml/81525.xml",downloadPdfUrl:"/chapter/pdf-download/81525",previewPdfUrl:"/chapter/pdf-preview/81525",totalDownloads:66,totalViews:0,totalCrossrefCites:0,dateSubmitted:"March 21st 2022",dateReviewed:"April 4th 2022",datePrePublished:"April 26th 2022",datePublished:null,dateFinished:"April 26th 2022",readingETA:"0",abstract:"Soybean, which has many foods, feed, and industrial raw material products, has relatively limited genetic diversity due to the domestication practices which mainly focused on higher yield for many centuries. Besides, cleistogamy in soybean plant reduces genetic variations even further. Improving genetic variation in soybean is crucial for breeding applications to improve traits such as higher yield, early maturity, herbicide, and pest resistance, lodging and shattering resistance, seed quality and composition, abiotic stress tolerance and more. In the 21st century, there are numerous alternatives from conventional breeding to biotechnological approaches. Among these, mutation breeding is still a major method to produce new alleles and desired traits within the crop genomes. Physical and chemical mutagen protocols are still improving and mutation breeding proves its value to be fast, flexible, and viable in crop sciences. In the verge of revolutionary genome editing era, induced mutagenesis passed important cross-roads successfully with the help of emerging supportive NGS based-methods and non-destructive screening approaches that reduce the time-consuming labor-intensive selection practices of mutation breeding. Induced mutagenesis will retain its place in crop science in the next decades, especially for plants such as soybean for which cross breeding is limited or not applicable.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/81525",risUrl:"/chapter/ris/81525",signatures:"Alp Ayan, Sinan Meriç, Tamer Gümüş and Çimen Atak",book:{id:"11364",type:"book",title:"Soybean - Recent Advances in Research and Applications",subtitle:null,fullTitle:"Soybean - Recent Advances in Research and Applications",slug:null,publishedDate:null,bookSignature:"Prof. Takuji Ohyama, Dr. Yoshihiko Takahashi, Dr. Norikuni Ohtake, Dr. Takashi Sato and Dr. Sayuri Tanabata",coverURL:"https://cdn.intechopen.com/books/images_new/11364.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-80355-700-7",printIsbn:"978-1-80355-699-4",pdfIsbn:"978-1-80355-701-4",isAvailableForWebshopOrdering:!0,editors:[{id:"30061",title:"Prof.",name:"Takuji",middleName:null,surname:"Ohyama",slug:"takuji-ohyama",fullName:"Takuji Ohyama"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:[{id:"147364",title:"Prof.",name:"Çimen",middleName:null,surname:"Atak",fullName:"Çimen Atak",slug:"cimen-atak",email:"c.atak@iku.edu.tr",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Istanbul Kültür University",institutionURL:null,country:{name:"Turkey"}}},{id:"191695",title:"Dr.",name:"Alp",middleName:null,surname:"Ayan",fullName:"Alp Ayan",slug:"alp-ayan",email:"a.ayan@iku.edu.tr",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/191695/images/14607_n.jpg",institution:{name:"Istanbul Kültür University",institutionURL:null,country:{name:"Turkey"}}},{id:"191696",title:"Dr.",name:"Sinan",middleName:null,surname:"Meriç",fullName:"Sinan Meriç",slug:"sinan-meric",email:"s.meric@iku.edu.tr",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Istanbul Kültür University",institutionURL:null,country:{name:"Turkey"}}},{id:"333168",title:"MSc.",name:"Tamer",middleName:null,surname:"Gümüş",fullName:"Tamer Gümüş",slug:"tamer-gumus",email:"t.gumus@iku.edu.tr",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Istanbul Kültür University",institutionURL:null,country:{name:"Turkey"}}}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Improving genomic variations",level:"1"},{id:"sec_2_2",title:"2.1 Conventional methods",level:"2"},{id:"sec_3_2",title:"2.2 Biotechnological approaches",level:"2"},{id:"sec_4_2",title:"2.3 Mutation breeding",level:"2"},{id:"sec_4_3",title:"2.3.1 Mutagens",level:"3"},{id:"sec_5_3",title:"2.3.2 Present applications of mutation breeding in soybean",level:"3"},{id:"sec_8",title:"3. Future Prospect and conclusion",level:"1"}],chapterReferences:[{id:"B1",body:'Knowlton S. High-oleic soybean oil. In: Flider FJ, editor. High Oleic Oils. IL, USA: AOCS Press; 2022. pp. 53-87. DOI: 10.1016/B978-0-12-822912-5.00007-1'},{id:"B2",body:'Willis S. The use of soybean meal and full fat soybean meal by the animal feed industry. In: 12th Australian Soybean Conference. Bundaberg: Soy Australia; 2003'},{id:"B3",body:'Costantini M, Bacenetti J. Soybean and maize cultivation in South America: Environmental comparison of different cropping systems. 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Plant Biotechnology Journal. 2018;16(1):176-185. DOI: 10.1111/pbi.12758'},{id:"B71",body:'Wang J, Kuang H, Zhang Z, Yang Y, Yan L, Zhang M, et al. Generation of seed lipoxygenase-free soybean using CRISPR-Cas9. The Crop Journal. 2020;8(3):432-439. DOI: 10.1016/j.cj.2019.08.008'},{id:"B72",body:'Sugano S, Hirose A, Kanazashi Y, Adachi K, Hibara M, Itoh T, et al. Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis. BMC Plant Biology. 2020;20(1):1-5. DOI: 10.1186/s12870-020-02708-6'},{id:"B73",body:'Zhang L, Wang Y, Li T, Qiu H, Xia Z, Dong Y. Target-specific mutations efficiency at multiple loci of CRISPR/Cas9 system using one sgRNA in soybean. Transgenic Research. 2021;30(1):51-62. DOI: 10.1007/s11248-020-00228-5'},{id:"B74",body:'Ma J, Sun S, Whelan J, Shou H. CRISPR/Cas9-mediated knockout of GmFATB1 significantly reduced the amount of saturated fatty acids in soybean seeds. International Journal of Molecular Sciences. 2021;22(8):3877. DOI: 10.3390/ijms22083877'},{id:"B75",body:'Han J, Guo B, Guo Y, Zhang B, Wang X, Qiu LJ. Creation of early flowering germplasm of soybean by CRISPR/Cas9 technology. Frontiers in Plant Science. 2019;1446. DOI: 10.3389/fpls.2019.01446'},{id:"B76",body:'Bao A, Chen H, Chen L, Chen S, Hao Q , Guo W, et al. CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean. BMC Plant Biology. 2019;19(1):1-2. DOI: 10.1186/s12870-019-1746-6'},{id:"B77",body:'Yang C, Huang Y, Lv W, Zhang Y, Bhat JA, Kong J, et al. GmNAC8 acts as a positive regulator in soybean drought stress. Plant Science. 2020;293:110442. DOI: 10.1016/j.plantsci.2020.110442'},{id:"B78",body:'Li Z, Liu ZB, Xing A, Moon BP, Koellhoffer JP, Huang L, et al. Cas9-guide RNA directed genome editing in soybean. Plant Physiology. 2015;169(2):960-970. DOI: 10.1104/pp.15.00783'},{id:"B79",body:'Schunk CR, Eberius M. 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Indexing and listing across major repositories, see details ...
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Live Performance Metrics to track readership and the impact of your chapter
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Dissemination and Promotion
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Aalborg University has Two Satellite Campuses, one in Copenhagen (Aalborg University Copenhagen) and the other in Esbjerg (Aalborg University Esbjerg).\n· He is a member of prestigious IEEE (Institute of Electrical and Electronics Engineers), and IAENG (International Association of Engineers) organizations. \n· He is the chief Editor of the Journal of Software Engineering.\n· He is the member of the Editorial Board of International Journal of Computer Science and Software Technology (IJCSST) and International Journal of Computer Engineering and Information Technology. \n· He is also the Editor of Communication in Computer and Information Science CCIS-20 by Springer.\n· Reviewer For Many Conferences\nHe is the lead person in making collaboration agreements between Aalborg University and many universities of Pakistan, for which the MOU’s (Memorandum of Understanding) have been signed.\nProfessor Akbar is working in Academia since 1990, he started his career as a Lab demonstrator/TA at the University of Sussex. After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. 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Everyone must undergo this phase of life at his or her own time and pace. In the broader sense, ageing reflects all the changes taking place over the course of life. These changes start from birth—one grows, develops and attains maturity. To the young, ageing is exciting. Middle age is the time when people notice the age-related changes like greying of hair, wrinkled skin and a fair amount of physical decline. Even the healthiest, aesthetically fit cannot escape these changes. Slow and steady physical impairment and functional disability are noticed resulting in increased dependency in the period of old age. According to World Health Organization, ageing is a course of biological reality which starts at conception and ends with death. It has its own dynamics, much beyond human control. However, this process of ageing is also subject to the constructions by which each society makes sense of old age. In most of the developed countries, the age of 60 is considered equivalent to retirement age and it is said to be the beginning of old age. In this chapter, you understand the details of ageing processes and associated physiological changes.",book:{id:"6381",slug:"gerontology",title:"Gerontology",fullTitle:"Gerontology"},signatures:"Shilpa Amarya, Kalyani Singh and Manisha Sabharwal",authors:[{id:"226573",title:"Ph.D.",name:"Shilpa",middleName:null,surname:"Amarya",slug:"shilpa-amarya",fullName:"Shilpa Amarya"},{id:"226593",title:"Dr.",name:"Kalyani",middleName:null,surname:"Singh",slug:"kalyani-singh",fullName:"Kalyani Singh"},{id:"243264",title:"Dr.",name:"Manisha",middleName:null,surname:"Sabharwal",slug:"manisha-sabharwal",fullName:"Manisha Sabharwal"}]},{id:"55388",doi:"10.5772/intechopen.68944",title:"Beauty, Body Image, and the Media",slug:"beauty-body-image-and-the-media",totalDownloads:7768,totalCrossrefCites:5,totalDimensionsCites:12,abstract:"This chapter analyses the role of the mass media in people’s perceptions of beauty. We summarize the research literature on the mass media, both traditional media and online social media, and how they appear to interact with psychological factors to impact appearance concerns and body image disturbances. There is a strong support for the idea that traditional forms of media (e.g. magazines and music videos) affect perceptions of beauty and appearance concerns by leading women to internalize a very slender body type as ideal or beautiful. Rather than simply being passive recipients of unrealistic beauty ideals communicated to them via the media, a great number of individuals actually seek out idealized images in the media. Finally, we review what is known about the role of social media in impacting society’s perception of beauty and notions of idealized physical forms. Social media are more interactive than traditional media and the effects of self‐presentation strategies on perceptions of beauty have just begun to be studied. This is an emerging area of research that is of high relevance to researchers and clinicians interested in body image and appearance concerns.",book:{id:"5925",slug:"perception-of-beauty",title:"Perception of Beauty",fullTitle:"Perception of Beauty"},signatures:"Jennifer S. Mills, Amy Shannon and Jacqueline Hogue",authors:[{id:"202110",title:"Dr.",name:"Jennifer S.",middleName:null,surname:"Mills",slug:"jennifer-s.-mills",fullName:"Jennifer S. Mills"}]},{id:"59227",doi:"10.5772/intechopen.73385",title:"Differentiating Normal Cognitive Aging from Cognitive Impairment No Dementia: A Focus on Constructive and Visuospatial Abilities",slug:"differentiating-normal-cognitive-aging-from-cognitive-impairment-no-dementia-a-focus-on-constructive",totalDownloads:1353,totalCrossrefCites:3,totalDimensionsCites:6,abstract:"Constructive and visuospatial abilities in normal and in pathological aging (cognitive impairment, no dementia, CIND) are investigated. The sample includes 188 participants over 60 years of age, divided in 2 groups: healthy subjects (MMSE ≥28), without cognitive complaints, and individuals with CIND (MMSE between 24 and 27 and subjective cognitive complains). Drawing of cube and drawing of house, Benton Visual Retention Test (BVRT), and Block design are used to test the hypothesis that short visuoconstructive and visuospatial tests can distinguish normal from pathological cognitive aging in its very early stages. Results proved the discriminative sensitivity of BVRT general assessment criteria and of omissions and distortions in CIND. The diagnostic sensitivity of a modification of Moore and Wike [1984] scoring system for house and cube drawing tasks was confirmed as well. Drawing of cube and house could be used for quick screening of CIND in subjects over 60. Principal component analysis with oblimin rotation was performed to explore the different dimensions in the visuospatial and visuoconstructive abilities in old age. A four-factor structure was established, all four factors explaining 71% of the variance.",book:{id:"6381",slug:"gerontology",title:"Gerontology",fullTitle:"Gerontology"},signatures:"Radka Ivanova Massaldjieva",authors:[{id:"75907",title:"Associate Prof.",name:"Radka Ivanova",middleName:null,surname:"Massaldjieva",slug:"radka-ivanova-massaldjieva",fullName:"Radka Ivanova Massaldjieva"}]},{id:"59658",doi:"10.5772/intechopen.74748",title:"Ageing Better in the Netherlands",slug:"ageing-better-in-the-netherlands",totalDownloads:1193,totalCrossrefCites:1,totalDimensionsCites:4,abstract:"The Dutch National Care for the Elderly Programme was an initiative organized by the Netherlands Organisation for Health Research and Development (ZonMw) between 2008 and 2016. The aim of the programme was to collect knowledge about frail elderly, to assess their needs and to provide person-centred and integrated care better suited to their needs. The budget of EUR 88 million was provided by the Dutch Ministry of Health, Welfare and Sports. Putting the needs of elderly people at the heart of the programme and ensuring their active participation were key to the programme’s success. The programme outcomes included the establishment of eight geriatric networks around the medical universities with 650 organisations and the completion of 218 projects. These projects, involving 43,000 elderly people and 8500 central caregivers, resulted in the completion of 45 PhD theses and the publication of more than 400 articles and the development of 300 practice toolkits, one database and a website, www.beteroud.nl. The Dutch National Care for the Elderly Programme has since developed into a movement and continues under the consortium Ageing Better, made up of eight organisations. Through the use of ambassadors, Ageing Better promotes the message that ageing is not a disease but a new phase of life.",book:{id:"6381",slug:"gerontology",title:"Gerontology",fullTitle:"Gerontology"},signatures:"Betty Meyboom-de Jong, Klaske Wynia and Anjo Geluk-Bleumink",authors:[{id:"224997",title:"Emeritus Prof.",name:"Betty",middleName:null,surname:"Meyboom-De Jong",slug:"betty-meyboom-de-jong",fullName:"Betty Meyboom-De Jong"},{id:"232900",title:"Dr.",name:"Klaske",middleName:null,surname:"Wynia",slug:"klaske-wynia",fullName:"Klaske Wynia"},{id:"232901",title:"Mrs.",name:"Anjo",middleName:null,surname:"Geluk-Bleumink",slug:"anjo-geluk-bleumink",fullName:"Anjo Geluk-Bleumink"}]},{id:"55890",doi:"10.5772/intechopen.69529",title:"Mindfulness Meditation and the Perception of Beauty: Implications for an Ecological Well-Being",slug:"mindfulness-meditation-and-the-perception-of-beauty-implications-for-an-ecological-well-being",totalDownloads:1428,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"Meditation is a first-person method for contemplating ourselves and the world, with more than 2500 years of history, rooted in the philosophical and contemplative traditions of the east. The present chapter aims to explore this worldview in order to demonstrate its relevance to our capacity for the appreciation of beauty. To this end, the aesthetic experience, the contemplative experience and their relationship with the practice of mindfulness are analysed. We suggest that the contemplative meditative experience bestows a state of consciousness and acceptance of life which places the practitioner in a progressive encounter with a self-concept that begins to detach from a static sense of the self and from the categories that define it, so that it may be experienced as an ongoing mental event, removed from cultural ideals of beauty or positivity. The result of this de-identification from the static self is a greater degree of psychological flexibility and a more genuine way of seeing the world, leading to a new perception of the self that is connected to an experience of freedom, and contributes to one’s own well-being, as well as to that of others and of the environment.",book:{id:"5925",slug:"perception-of-beauty",title:"Perception of Beauty",fullTitle:"Perception of Beauty"},signatures:"Álvaro I. Langer, Carlos Schmidt and Edwin Krogh",authors:[{id:"199843",title:"Dr.",name:"Álvaro",middleName:null,surname:"Langer",slug:"alvaro-langer",fullName:"Álvaro Langer"},{id:"201865",title:"MSc.",name:"Carlos",middleName:null,surname:"Schmidt",slug:"carlos-schmidt",fullName:"Carlos Schmidt"},{id:"201866",title:"Dr.",name:"Edwin",middleName:null,surname:"Krogh",slug:"edwin-krogh",fullName:"Edwin Krogh"}]}],mostDownloadedChaptersLast30Days:[{id:"60564",title:"Ageing Process and Physiological Changes",slug:"ageing-process-and-physiological-changes",totalDownloads:6996,totalCrossrefCites:19,totalDimensionsCites:34,abstract:"Ageing is a natural process. Everyone must undergo this phase of life at his or her own time and pace. In the broader sense, ageing reflects all the changes taking place over the course of life. These changes start from birth—one grows, develops and attains maturity. To the young, ageing is exciting. Middle age is the time when people notice the age-related changes like greying of hair, wrinkled skin and a fair amount of physical decline. Even the healthiest, aesthetically fit cannot escape these changes. Slow and steady physical impairment and functional disability are noticed resulting in increased dependency in the period of old age. According to World Health Organization, ageing is a course of biological reality which starts at conception and ends with death. It has its own dynamics, much beyond human control. However, this process of ageing is also subject to the constructions by which each society makes sense of old age. In most of the developed countries, the age of 60 is considered equivalent to retirement age and it is said to be the beginning of old age. In this chapter, you understand the details of ageing processes and associated physiological changes.",book:{id:"6381",slug:"gerontology",title:"Gerontology",fullTitle:"Gerontology"},signatures:"Shilpa Amarya, Kalyani Singh and Manisha Sabharwal",authors:[{id:"226573",title:"Ph.D.",name:"Shilpa",middleName:null,surname:"Amarya",slug:"shilpa-amarya",fullName:"Shilpa Amarya"},{id:"226593",title:"Dr.",name:"Kalyani",middleName:null,surname:"Singh",slug:"kalyani-singh",fullName:"Kalyani Singh"},{id:"243264",title:"Dr.",name:"Manisha",middleName:null,surname:"Sabharwal",slug:"manisha-sabharwal",fullName:"Manisha Sabharwal"}]},{id:"55388",title:"Beauty, Body Image, and the Media",slug:"beauty-body-image-and-the-media",totalDownloads:7764,totalCrossrefCites:5,totalDimensionsCites:12,abstract:"This chapter analyses the role of the mass media in people’s perceptions of beauty. We summarize the research literature on the mass media, both traditional media and online social media, and how they appear to interact with psychological factors to impact appearance concerns and body image disturbances. There is a strong support for the idea that traditional forms of media (e.g. magazines and music videos) affect perceptions of beauty and appearance concerns by leading women to internalize a very slender body type as ideal or beautiful. Rather than simply being passive recipients of unrealistic beauty ideals communicated to them via the media, a great number of individuals actually seek out idealized images in the media. Finally, we review what is known about the role of social media in impacting society’s perception of beauty and notions of idealized physical forms. Social media are more interactive than traditional media and the effects of self‐presentation strategies on perceptions of beauty have just begun to be studied. This is an emerging area of research that is of high relevance to researchers and clinicians interested in body image and appearance concerns.",book:{id:"5925",slug:"perception-of-beauty",title:"Perception of Beauty",fullTitle:"Perception of Beauty"},signatures:"Jennifer S. Mills, Amy Shannon and Jacqueline Hogue",authors:[{id:"202110",title:"Dr.",name:"Jennifer S.",middleName:null,surname:"Mills",slug:"jennifer-s.-mills",fullName:"Jennifer S. Mills"}]},{id:"56505",title:"Aesthetics of the Naked Human Body: From Pornography (Sexualised Lust Object) to Iconography (Aesthetics of Human Nobility and Wisdom) in an Anthropology of Physical Beauty",slug:"aesthetics-of-the-naked-human-body-from-pornography-sexualised-lust-object-to-iconography-aesthetics",totalDownloads:2100,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"In many religious circles and philosophies of life, the human body is excluded from the realm of spirituality and meaning. Due to a dualistic approach, nudity is viewed as merely a physical and corporeal category. In social media, there is the real danger that the naked human body is exploited for commercial gain. Advertisements often leave the impression that the body, very specifically the genitals, is designed merely for physical desire and corporeal chemistry. They become easily objects for lust, excluded from the beauty of graceful existence and noble courage. It is argued that the naked human body is not designed for pornographic exploitation and promiscuous sensuality but for compassionate intimacy and nurturing care in order to instil a humane dimension in human and sexual encounters. In this regard, antiquity and the Michelangelesque perspective can contribute to a paradigm shift from abusive exploitation to the beauty of vulnerable sensitivity. In order to foster an integrative approach to theory formation in anthropology, the methodology of stereometric thinking is proposed.",book:{id:"5925",slug:"perception-of-beauty",title:"Perception of Beauty",fullTitle:"Perception of Beauty"},signatures:"Daniel J Louw",authors:[{id:"200645",title:"Prof.",name:"Daniel",middleName:"Johannes",surname:"Louw",slug:"daniel-louw",fullName:"Daniel Louw"}]},{id:"56059",title:"A Plastic Surgeon’s Perspective on Stereotyping and the Perception of Beauty",slug:"a-plastic-surgeon-s-perspective-on-stereotyping-and-the-perception-of-beauty",totalDownloads:1918,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"In the world of plastic surgery, misconceptions may lead to irrational requests or outcomes not appreciated by patients. Those who manage aesthetics should always listen and recognize the variability of cultural identities, desires, attitudes, anxieties and uncertainties of the patient. Emerging from a diversity of cultures and its transforming trends, the scope of cosmetic surgery and its practice reflect not only the individual’s personality, but also the culture as a whole. When counseling an individual, one has to recognize that even in groups of seemingly identical social or cultural standards; there are subtle differences in expectations. To illustrate the potential for inaccuracy of ethnic profiling in the field of plastic surgery authors quote their own work on Asian subjects and facial beauty and resort to experience of others. To reaffirm their opinion and to exemplify how sometimes “fine” differences in the perception of beauty exist, an original study that evaluates the preferences among selected groups of Latina women in respect to buttock aesthetics has been included. This dissertation will focus on how cultural factors influence beauty perception; strengthen the fact that beauty is in the eye of the beholder and how variable differences exist even between small subgroups.",book:{id:"5925",slug:"perception-of-beauty",title:"Perception of Beauty",fullTitle:"Perception of Beauty"},signatures:"Johanna D’Agostino and Marek Dobke",authors:[{id:"17590",title:"Dr.",name:"Marek K.",middleName:null,surname:"Dobke",slug:"marek-k.-dobke",fullName:"Marek K. Dobke"},{id:"201244",title:"Dr.",name:"Johanna",middleName:null,surname:"D'Agostino",slug:"johanna-d'agostino",fullName:"Johanna D'Agostino"}]},{id:"80326",title:"Anti-Senescence Therapy",slug:"anti-senescence-therapy",totalDownloads:110,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"The development of therapeutic strategies aimed at the aging process of cells has attracted increasing attention in recent decades due to the involvement of this process in the development of many chronic and age-related diseases. Interestingly, preclinical studies have shown the success of a number of anti-aging approaches in the treatment of a range of chronic diseases. These approaches are directed against aging processes such as oxidative stress, telomerase shortening, inflammation, and deficient autophagy. Many strategies has been shown to be effective in delaying aging, including antiaging strategies based on establishing healthy lifestyle habits and pharmacological interventions aimed at disrupting senescent cells and senescent-associated secretory phenotype. Caloric restriction and intermittent fasting were reported to activate autophagy and reduce inflammation. In turn, immune-based strategies, senolytic agents, and senomorphics mediate their effects either by eliminating senescent cells through inducing apoptosis or by disrupting pathways by which senescent cells mediate their detrimental effects. In addition, given the association of the decline in the regenerative potential of stem cells with aging, many experimental and clinical studies indicate the effectiveness of stem cell transplantation in preventing or slowing the progress of age-related diseases by enhancing the repairing mechanisms and the secretion of many growth factors and cytokines.",book:{id:"10935",slug:null,title:"Mechanisms and Management of Senescence",fullTitle:"Mechanisms and Management of Senescence"},signatures:"Raghad Alshadidi",authors:null}],onlineFirstChaptersFilter:{topicId:"235",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82112",title:"Comparative Senescence and Lifespan",slug:"comparative-senescence-and-lifespan",totalDownloads:17,totalDimensionsCites:0,doi:"10.5772/intechopen.105137",abstract:"The word senescence is derived from the Latin word “senex” (meaning old). In biology, senescence is a process by which a cell ages and permanently stops dividing. Senescence is a natural universal phenomenon affecting all living organisms (e.g., humans, animals, and plants). It is the process of growing old (aging). The underlying mechanisms of senescence and aging at the cellular level are not fully understood. Senescence is a multifactorial process that can be induced by several stimuli including cellular stress, DNA damage, telomere shortening, and oncogene activation. The most popular theory to explain aging is the free radical theory. Senescence plays a role in the development of several age-related chronic diseases in humans (e.g., ischemic heart disease, osteoporosis, and cancer). Lifespan is a biological characteristic of every species. The lifespan of living organisms ranges from few hours (with mayfly) to potential eternity (with jellyfish and hydra). The maximum theoretical lifespan in humans is around 120 years. The lifespan in humans is influenced by multiple factors including genetic, epigenetic, lifestyle, environmental, metabolic, and endocrine factors. There are several ways to potentially extend the lifespan of humans and eventually surpass the maximum theoretical lifespan of 120 years. The tools that can be proposed include lifestyle, reduction of several life-threatening diseases and disabilities, hormonal replacement, antioxidants, autophagy inducers, senolytic drugs, stem cell therapy, and gene therapy.",book:{id:"10935",title:"Mechanisms and Management of Senescence",coverURL:"https://cdn.intechopen.com/books/images_new/10935.jpg"},signatures:"Hassan M. Heshmati"},{id:"81638",title:"Aging and Neuropsychiatric Disease: A General Overview of Prevalence and Trends",slug:"aging-and-neuropsychiatric-disease-a-general-overview-of-prevalence-and-trends",totalDownloads:30,totalDimensionsCites:0,doi:"10.5772/intechopen.103102",abstract:"The increasing trend of life-expectancy is becoming a significant demographic, societal and economic challenge. Currently, global number of people above sixty years of age is 900 million, while United Nations expect this number to rise to over 1.4 billion in 2030 and over 2.5 billion by 2050. Concordant to this trend, numerous physiological changes are associated with aging and brain-related ones are associated with neuropsychiatric diseases. The main goal of this chapter is to identify the most important neuropsychiatric diseases to assess in older patients to help to promote health and prevent diseases and complications associated with chronic illness, as these changes are progressive and require important psychological and setting-related social adjustments. Findings identify several health-aspects highly present in elderly: stroke, white matter lesions, dementia rise with age, changes in levels of neurotransmitters and hormones, depression as well as the bereavement following loss of the loved one, and the most common neurodegenerative disease—Alzheimer’s disease and Parkinson’s. In conclusion, studying the aging process should include all developmental, circumstantial, and individual aspects of aging. This offers opportunities to improve the health of elderly by using a wide range of skills and knowledge. Thus, further studies are necessary to elucidate what can be done do to improve the aging process and health of elderly in the future.",book:{id:"10935",title:"Mechanisms and Management of Senescence",coverURL:"https://cdn.intechopen.com/books/images_new/10935.jpg"},signatures:"Jelena Milić"},{id:"80326",title:"Anti-Senescence Therapy",slug:"anti-senescence-therapy",totalDownloads:110,totalDimensionsCites:0,doi:"10.5772/intechopen.101585",abstract:"The development of therapeutic strategies aimed at the aging process of cells has attracted increasing attention in recent decades due to the involvement of this process in the development of many chronic and age-related diseases. Interestingly, preclinical studies have shown the success of a number of anti-aging approaches in the treatment of a range of chronic diseases. These approaches are directed against aging processes such as oxidative stress, telomerase shortening, inflammation, and deficient autophagy. Many strategies has been shown to be effective in delaying aging, including antiaging strategies based on establishing healthy lifestyle habits and pharmacological interventions aimed at disrupting senescent cells and senescent-associated secretory phenotype. Caloric restriction and intermittent fasting were reported to activate autophagy and reduce inflammation. In turn, immune-based strategies, senolytic agents, and senomorphics mediate their effects either by eliminating senescent cells through inducing apoptosis or by disrupting pathways by which senescent cells mediate their detrimental effects. In addition, given the association of the decline in the regenerative potential of stem cells with aging, many experimental and clinical studies indicate the effectiveness of stem cell transplantation in preventing or slowing the progress of age-related diseases by enhancing the repairing mechanisms and the secretion of many growth factors and cytokines.",book:{id:"10935",title:"Mechanisms and Management of Senescence",coverURL:"https://cdn.intechopen.com/books/images_new/10935.jpg"},signatures:"Raghad Alshadidi"},{id:"79828",title:"Cellular Senescence in Bone",slug:"cellular-senescence-in-bone",totalDownloads:119,totalDimensionsCites:0,doi:"10.5772/intechopen.101803",abstract:"Senescence is an irreversible cell-cycle arrest process induced by environmental, genetic, and epigenetic factors. An accumulation of senescent cells in bone results in age-related disorders, and one of the common problems is osteoporosis. Deciphering the basic mechanisms contributing to the chronic ailments of aging may uncover new avenues for targeted treatment. This review focuses on the mechanisms and the most relevant research advancements in skeletal cellular senescence. To identify new options for the treatment or prevention of age-related chronic diseases, researchers have targeted hallmarks of aging, including telomere attrition, genomic instability, cellular senescence, and epigenetic alterations. First, this chapter provides an overview of the fundamentals of bone tissue, the causes of skeletal involution, and the role of cellular senescence in bone and bone diseases such as osteoporosis. Next, this review will discuss the utilization of pharmacological interventions in aging tissues and, more specifically, highlight the role of senescent cells to identify the most effective and safe strategies.",book:{id:"10935",title:"Mechanisms and Management of Senescence",coverURL:"https://cdn.intechopen.com/books/images_new/10935.jpg"},signatures:"Danielle Wang and Haitao Wang"},{id:"79668",title:"Identification of RNA Species That Bind to the hnRNP A1 in Normal and Senescent Human Fibroblasts",slug:"identification-of-rna-species-that-bind-to-the-hnrnp-a1-in-normal-and-senescent-human-fibroblasts",totalDownloads:81,totalDimensionsCites:0,doi:"10.5772/intechopen.101525",abstract:"hnRNP A1 is a member of the hnRNPs (heterogeneous nuclear ribonucleoproteins) family of proteins that play a central role in regulating genes responsible for cell proliferation, DNA repair, apoptosis, and telomere biogenesis. Previous studies have shown that hnRNPA1 had reduced protein levels and increased cytoplasmic accumulation in senescent human diploid fibroblasts. The consequence of reduced protein expression and altered cellular localization may account for the alterations in gene expression observed during senescence. There is limited information for gene targets of hnRNP A1 as well as its in vivo function. In these studies, we performed RNA co-immunoprecipitation experiments using hnRNP A1 as the target protein to identify potential mRNA species in ribonucleoprotein (RNP) complexes. Using this approach, we identified the human double minute 2 (HDM2) mRNA as a binding target for hnRNP A1 in young and senescent human diploid fibroblasts cells. It was also observed that alterations of hnRNP A1 expression modulate HDM2 mRNA levels in young IMR-90 cells. We also demonstrated that the levels of HDM2 mRNA increased with the downregulation of hnRNP A1 and decrease with the overexpression of hnRNP A1. Although we did not observe a significant decrease in HDM2 protein level, a concomitant increase in p53 protein level was detected with the overexpression of hnRNP A1. Our studies also show that hnRNP A1 directly interacts with HDM2 mRNA at a region corresponding to its 3′ UTR (untranslated region of a gene). The results from this study demonstrate that hnRNP A1 has a novel role in participating in the regulation of HDM2 gene expression.",book:{id:"10935",title:"Mechanisms and Management of Senescence",coverURL:"https://cdn.intechopen.com/books/images_new/10935.jpg"},signatures:"Heriberto Moran, Shanaz A. Ghandhi, Naoko Shimada and Karen Hubbard"},{id:"79295",title:"Genetic and Epigenetic Influences on Cutaneous Cellular Senescence",slug:"genetic-and-epigenetic-influences-on-cutaneous-cellular-senescence",totalDownloads:136,totalDimensionsCites:0,doi:"10.5772/intechopen.101152",abstract:"Skin is the largest human organ system, and its protective function is critical to survival. The epithelial, dermal, and subcutaneous compartments are heterogeneous mixtures of cell types, yet they all display age-related skin dysfunction through the accumulation of an altered phenotypic cellular state called senescence. Cellular senescence is triggered by complex and dynamic genetic and epigenetic processes. A senescence steady state is achieved in different cell types under various and overlapping conditions of chronological age, toxic injury, oxidative stress, replicative exhaustion, DNA damage, metabolic dysfunction, and chromosomal structural changes. These inputs lead to outputs of cell-cycle withdrawal and the appearance of a senescence-associated secretory phenotype, both of which accumulate as tissue pathology observed clinically in aged skin. This review details the influence of genetic and epigenetic factors that converge on normal cutaneous cellular processes to create the senescent state, thereby dictating the response of the skin to the forces of both intrinsic and extrinsic aging. From this work, it is clear that no single biomarker or process leads to senescence, but that it is a convergence of factors resulting in an overt aging phenotype.",book:{id:"10935",title:"Mechanisms and Management of Senescence",coverURL:"https://cdn.intechopen.com/books/images_new/10935.jpg"},signatures:"Tapash Jay Sarkar, Maiko Hermsmeier, Jessica L. Ross and G. Scott Herron"}],onlineFirstChaptersTotal:6},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:0,limit:8,total:null},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:90,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:108,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:330,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:9,numberOfPublishedChapters:141,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:123,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:112,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:22,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:11,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"24",title:"Sustainable Development",doi:"10.5772/intechopen.100361",issn:"2753-6580",scope:"
\r\n\tTransforming our World: the 2030 Agenda for Sustainable Development endorsed by United Nations and 193 Member States, came into effect on Jan 1, 2016, to guide decision making and actions to the year 2030 and beyond. Central to this Agenda are 17 Goals, 169 associated targets and over 230 indicators that are reviewed annually. The vision envisaged in the implementation of the SDGs is centered on the five Ps: People, Planet, Prosperity, Peace and Partnership. This call for renewed focused efforts ensure we have a safe and healthy planet for current and future generations.
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\r\n\tThis Series focuses on covering research and applied research involving the five Ps through the following topics:
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\r\n\t1. Sustainable Economy and Fair Society that relates to SDG 1 on No Poverty, SDG 2 on Zero Hunger, SDG 8 on Decent Work and Economic Growth, SDG 10 on Reduced Inequalities, SDG 12 on Responsible Consumption and Production, and SDG 17 Partnership for the Goals
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\r\n\t2. Health and Wellbeing focusing on SDG 3 on Good Health and Wellbeing and SDG 6 on Clean Water and Sanitation
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\r\n\t3. Inclusivity and Social Equality involving SDG 4 on Quality Education, SDG 5 on Gender Equality, and SDG 16 on Peace, Justice and Strong Institutions
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\r\n\t4. Climate Change and Environmental Sustainability comprising SDG 13 on Climate Action, SDG 14 on Life Below Water, and SDG 15 on Life on Land
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\r\n\t5. Urban Planning and Environmental Management embracing SDG 7 on Affordable Clean Energy, SDG 9 on Industry, Innovation and Infrastructure, and SDG 11 on Sustainable Cities and Communities.
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\r\n\tThe series also seeks to support the use of cross cutting SDGs, as many of the goals listed above, targets and indicators are all interconnected to impact our lives and the decisions we make on a daily basis, making them impossible to tie to a single topic.
",coverUrl:"https://cdn.intechopen.com/series/covers/24.jpg",latestPublicationDate:"August 2nd, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:1,editor:{id:"262440",title:"Prof.",name:"Usha",middleName:null,surname:"Iyer-Raniga",slug:"usha-iyer-raniga",fullName:"Usha Iyer-Raniga",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRYSXQA4/Profile_Picture_2022-02-28T13:55:36.jpeg",biography:"Usha Iyer-Raniga is a professor in the School of Property and Construction Management at RMIT University. Usha co-leads the One Planet Network’s Sustainable Buildings and Construction Programme (SBC), a United Nations 10 Year Framework of Programmes on Sustainable Consumption and Production (UN 10FYP SCP) aligned with Sustainable Development Goal 12. The work also directly impacts SDG 11 on Sustainable Cities and Communities. She completed her undergraduate degree as an architect before obtaining her Masters degree from Canada and her Doctorate in Australia. Usha has been a keynote speaker as well as an invited speaker at national and international conferences, seminars and workshops. Her teaching experience includes teaching in Asian countries. She has advised Austrade, APEC, national, state and local governments. She serves as a reviewer and a member of the scientific committee for national and international refereed journals and refereed conferences. She is on the editorial board for refereed journals and has worked on Special Issues. Usha has served and continues to serve on the Boards of several not-for-profit organisations and she has also served as panel judge for a number of awards including the Premiers Sustainability Award in Victoria and the International Green Gown Awards. Usha has published over 100 publications, including research and consulting reports. Her publications cover a wide range of scientific and technical research publications that include edited books, book chapters, refereed journals, refereed conference papers and reports for local, state and federal government clients. She has also produced podcasts for various organisations and participated in media interviews. She has received state, national and international funding worth over USD $25 million. Usha has been awarded the Quarterly Franklin Membership by London Journals Press (UK). Her biography has been included in the Marquis Who's Who in the World® 2018, 2016 (33rd Edition), along with approximately 55,000 of the most accomplished men and women from around the world, including luminaries as U.N. Secretary-General Ban Ki-moon. In 2017, Usha was awarded the Marquis Who’s Who Lifetime Achiever Award.",institutionString:null,institution:{name:"RMIT University",institutionURL:null,country:{name:"Australia"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:5,paginationItems:[{id:"91",title:"Sustainable Economy and Fair Society",coverUrl:"https://cdn.intechopen.com/series_topics/covers/91.jpg",isOpenForSubmission:!0,editor:{id:"181603",title:"Dr.",name:"Antonella",middleName:null,surname:"Petrillo",slug:"antonella-petrillo",fullName:"Antonella Petrillo",profilePictureURL:"https://mts.intechopen.com/storage/users/181603/images/system/181603.jpg",biography:"Antonella Petrillo, Ph.D., is a professor in the Department of Engineering, University of Naples “Parthenope,” Italy. She received her Ph.D. in Mechanical Engineering from the University of Cassino and Southern Lazio, Italy. Her research interests include multi-criteria decision analysis, industrial plants, logistics, manufacturing, and safety. She serves as an associate editor for the International Journal of the Analytic Hierarchy Process and is an editorial board member for several other journals. She is also a member of the Analytic Hierarchy Process (AHP) Academy.",institutionString:"Parthenope University of Naples",institution:{name:"Parthenope University of Naples",institutionURL:null,country:{name:"Italy"}}},editorTwo:null,editorThree:null},{id:"92",title:"Health and Wellbeing",coverUrl:"https://cdn.intechopen.com/series_topics/covers/92.jpg",isOpenForSubmission:!0,editor:{id:"348225",title:"Prof.",name:"Ann",middleName:null,surname:"Hemingway",slug:"ann-hemingway",fullName:"Ann Hemingway",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035LZFoQAO/Profile_Picture_2022-04-11T14:55:40.jpg",biography:"Professor Hemingway is a public health researcher, Bournemouth University, undertaking international and UK research focused on reducing inequalities in health outcomes for marginalised and excluded populations and more recently focused on equine assisted interventions.",institutionString:null,institution:{name:"Bournemouth University",institutionURL:null,country:{name:"United Kingdom"}}},editorTwo:null,editorThree:null},{id:"93",title:"Inclusivity and Social Equity",coverUrl:"https://cdn.intechopen.com/series_topics/covers/93.jpg",isOpenForSubmission:!0,editor:{id:"210060",title:"Prof. Dr.",name:"Ebba",middleName:null,surname:"Ossiannilsson",slug:"ebba-ossiannilsson",fullName:"Ebba Ossiannilsson",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6LkBQAU/Profile_Picture_2022-02-28T13:31:48.png",biography:"Professor Dr. Ebba Ossiannilsson is an independent researcher, expert, consultant, quality auditor and influencer in the fields of open, flexible online and distance learning (OFDL) and the 'new normal'. Her focus is on quality, innovation, leadership, and personalised learning. She works primarily at the strategic and policy levels, both nationally and internationally, and with key international organisations. She is committed to promoting and improving OFDL in the context of SDG4 and the future of education. Ossiannilsson has more than 20 years of experience in her current field, but more than 40 years in the education sector. She works as a reviewer and expert for the European Commission and collaborates with the Joint Research Centre for Quality in Open Education. Ossiannilsson also collaborates with ITCILO and ICoBC (International Council on Badges and Credentials). She is a member of the ICDE Board of Directors and has previously served on the boards of EDEN and EUCEN. Ossiannilsson is a quality expert and reviewer for ICDE, EDEN and the EADTU. She chairs the ICDE OER Advocacy Committee and is a member of the ICDE Quality Network. She is regularly invited as a keynote speaker at conferences. She is a guest editor for several special issues and a member of the editorial board of several scientific journals. She has published more than 200 articles and is currently working on book projects in the field of OFDL. Ossiannilsson is a visiting professor at several international universities and was recently appointed Professor and Research Fellow at Victoria University of Wellington, NZ. Ossiannilsson has been awarded the following fellowships: EDEN Fellows, EDEN Council of Fellows, and Open Education Europe. She is a ICDE OER Ambassador, Open Education Europe Ambassador, GIZ Ambassador for Quality in Digital Learning, and part of the Globe-Community of Digital Learning and Champion of SPARC Europe. On a national level, she is a quality developer at the Swedish Institute for Standards (SIS) and for ISO. She is a member of the Digital Skills and Jobs Coalition Sweden and Vice President of the Swedish Association for Distance Education. She is currently working on a government initiative on quality in distance education at the National Council for Higher Education. She holds a Ph.D. from the University of Oulu, Finland.",institutionString:"Swedish Association for Distance Education, Sweden",institution:null},editorTwo:null,editorThree:null},{id:"94",title:"Climate Change and Environmental Sustainability",coverUrl:"https://cdn.intechopen.com/series_topics/covers/94.jpg",isOpenForSubmission:!0,editor:{id:"61855",title:"Dr.",name:"Yixin",middleName:null,surname:"Zhang",slug:"yixin-zhang",fullName:"Yixin Zhang",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYWJgQAO/Profile_Picture_2022-06-09T11:36:35.jpg",biography:"Professor Yixin Zhang is an aquatic ecologist with over 30 years of research and teaching experience in three continents (Asia, Europe, and North America) in Stream Ecology, Riparian Ecology, Urban Ecology, and Ecosystem Restoration and Aquatic Conservation, Human-Nature Interactions and Sustainability, Urbanization Impact on Aquatic Ecosystems. He got his Ph.D. in Animal Ecology at Umeå University in Sweden in 1998. He conducted postdoc research in stream ecology at the University of California at Santa Barbara in the USA. After that, he was a postdoc research fellow at the University of British Columbia in Canada to do research on large-scale stream experimental manipulation and watershed ecological survey in temperate rainforests of BC. He was a faculty member at the University of Hong Kong to run ecological research projects on aquatic insects, fishes, and newts in Tropical Asian streams. He also conducted research in streams, rivers, and caves in Texas, USA, to study the ecology of macroinvertebrates, big-claw river shrimp, fish, turtles, and bats. 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