Lichen species used in attempts of synthesis.
The late 19th and, especially, the early 20th century were marked by the introduction of experimental approaches in various biological disciplines. The methods of accumulative and axenic microorganism cultures were already widely used in microbiology of that period; in animal and plant sciences, attempts were made to grow whole organisms, individual organs, tissues and/or individual cells under controlled laboratory conditions (Vochting, 1892; Harrison, 1907). By the early 20th century, some results had already been achieved in cultivating animal tissues (Krontovsky, 1917 cited in Butenko, 1999), and, in the 1920s, plant and animal cells and tissues (Czech, 1927; Prat, 1927; Gautheret, 1932; White, 1932). An important step in plant tissue cultivation was the discovery of phytohormones and development of specialized cultivating media that allowed inducing, on the one hand, dedifferentiation and callus formation, or, on the other hand, cell differentiation. These achievements helped to solve a number of problems, both theoretical and applied (Street, 1977; Butenko, 1999). With time, the spectrum of organisms introduced in cultures was widening, the principal methods of growing plant cells
The said period was also marked by the formation and development of the notion of symbiosis. The revolutionary works of A.S. Famintsyn (1865) and S. Schwendener (1867) (as cited in Famintsyn, 1907) discovered the dual nature of lichens. The notion of symbiosis was formulated in 1879 by A. de Bary. In the early 20th century, K.S. Mereschkowski established the theory of symbiogenetic origin for the eukaryotic cell and formulated the notion of two "plasms" (Mereschkowski, 1907, 1909).
Symbiosis is currently studied by a special scientific discipline, symbiology, and regarded as a stable super-organism system undergoing balanced growth and characterized by specific interrelations of components, and by unique biochemistry and physiology (Ahmadjian & Paracer, 1986; Paracer & Ahmadjian, 2000).
It is noteworthy that the development of each of the above-mentioned fields of study has not been independent. Constantly intervening with each other, works in all these fields were conductive to the formation of a new branch, already within the new science of symbiology. In the 1990s, this new branch was termed experimental symbiology.
2. Specifics of lichens as experimental systems. Peculiarities of the terminology
Lichens are a classic example of symbiotic associations with multicomponent composition as their principal feature. According to the number of partners forming the thallus, two- and three-component lichens are recognized. The former consist of a fungal component (the mycobiont) and a photosynthetic component (the photobiont). In two-component lichens, the photobiont is represented either with a green alga or a cyanobacterium; in three-component lichens, with both: a green alga in the basal part of the thallus and a cyanobacterium in specialized formations, cephalodia (Rai, 1990, Paracer & Ahmadjian, 2000).
According to the type of localization in the lichen, internal (intra-thallus) and external (surface) cephalodia are recognized. In nature, lichens with internal cephalodia are probably prevalent. Some investigators, e.g., P.A. Genkel and L.A. Yuzhakova (1936) (the history of the question is described in: A.N. Oksner, 1974) suggested that nitrogen-fixing bacteria (such as
In addition to morphology, lichens as symbiotic systems demonstrate a number of peculiar biochemical and ecological features. Only occasional findings of the so-called lichen compounds in monocultures of lichen symbionts (in most cases, mycobionts) have been reported (Ahmadjian, 1961, 1967). At the same time, large amounts of phenolic compounds (mainly depsides and depsidones), found almost nowhere else, are present in lichens (Culberson, 1969; Vainshtein, 1982a, 1982b, 1982c). The functions of these compounds are not yet fully known. Various compounds probably play different roles in the vital functions of lichens: some participate in the initiation of symbiotic interactions (Ahmadjian, 1989), some provide for the exchange of nutrients between the symbionts (Vainshtein, 1988), and some are used for adaptation to environmental conditions (e.g., in substrate destruction or in competition: Tolpysheva, 1984a, 1984b, 1985; Vainshtein & Tolpysheva 1992; Manojlovic et. al., 2002). Symbiosis helped lichens to become extremely widespread, but they are prevalent in extreme or simply oligotrophic habitats. This probably reflects the fact that lichens are capable of surviving considerable changes of temperature, drying, poor substrates, but at the same time, due to slow growth, it is hard for them to survive competition with higher plants (Paracer & Ahmadjian, 2000).
The multicomponent composition of lichens makes it difficult to use them in biotechnology. Lichens are super-organism multicomponent systems, and we believe that it is necessary to discuss here the terminology used for growing lichens in culture. In English-language literature, the word "culture" is used for laboratory manipulations with lichen thalli and their fragments, but different authors understand this term differently. Taking into account the fact that experimental lichenology developed largely on the basis of approaches borrowed from plant physiology, we believe that it is advisable to define the notion of "culture" more accurately, in the light of the sense this term has in plant physiology, where it means the growing of dedifferentiated parts of an organism on growth media under controlled laboratory conditions (Street, 1977; Butenko, 1999). Lichens have no true tissues, only plectenchymas, and thus the term "tissues" can be used only in quotation marks. We believe that it is possible, by analogy, to use also a number of other terms, such as explants, "callus cultures", etc.
3. History and classification of the experimental approaches in lichenology
Two principal groups of experimental approaches can be recognized in lichenology (Fig. 1, 2): first, resynthesis of lichens and producing of model systems (developed by V. Ahmadjian, M. Galun), and second, obtaining lichen "tissue cultures" (developed by Y. Yamamoto). The former groups of approaches is based on the dissociation of the initial thallus into components (Fig. 1) and subsequent attempt to resynthesize the initial sample or model it based on similar systems (Ahmadjian, 1973a, 1973b). In the latter case, dedifferentiated biomasses of the lichen are obtained (Fig. 2), using thallus fragments, soredia, or isidia (Yoshimura & Yamamoto, 1991; Yoshimura et al., 1993; Yamamoto et al., 1993).
The former group of approaches reconstructs the general situation emerging in lichens in the course of sexual reproduction, and the latter resembles rather the processes that take place in nature in the course of their vegetative reproduction. For instance, soredia are dedifferentiated parts of the thallus consisting of cells of both symbionts, which makes them very convenient sources for obtaining "tissue cultures". Isidia, morphologically structured thallus fragments, are natural explants of microclonal propagation (Fig. 2). But soredia and isidia are found only in some lichens, which makes obtaining "lichen tissue cultures" from thallus fragments an important approach (Yamamoto et al., 1993; Yoshimura et al., 1993). Interestingly, lichen thalli develop similarly in all cases, independently of the approach used (Ahmadjian, 1973a, 1973b; Yoshimura et al., 1993; Stocker-Worgotter & Turk, 1989).
Attempts to synthesize lichens from separate components (fungi and green algae or cyanobacteria) were made simultaneously with the discovery of the dual nature of lichens by S. Schwendener in 1867. In the same year, A.S. Faminsyn and O.V. Baranetsky, cultivating fragments of lichen thalli, succeeded in describing for the first time the growth of the photobiont outside the thallus. However, almost all attempts to resynthesize a lichen from separate components in the late 19th century were unsuccessful (Bornet, 1873; Bonnier, 1889; critique of these works: Ahmadjian & Jacobs, 1983). The cause of these failures was the lack of both theoretical and experimental foundations laid for such experiments. Successful experiments on lichen construction were performed only three decades later, due to the development of theoretical data on the biology of lichens and laying of methodological foundations for cultivating lichen component monocultures on artificial media.
In 1939, E. Thomas was the first to successfully resynthesize the lichen
Lichenologists became considerably more interested in resynthesizing the thalli of two-component lichens thanks to the classic studies of Ahmadjian (1961, 1967, 1973a, 1973b, 1990). His contribution to this area is hard to overestimate: over 40% of publications on lichen thalli resynthesis were produced by him alone or in collaboration with others. He developed the method for extracting and cultivating the mycobiont from apothecia. Thanks to Ahmadjian's work, thallus resynthesis of some lichen species became common practice. The principal advantages and disadvantages of the methods he proposed are now clear, but while the advantages are currently widely used in experimental studies, the disadvantages have not yet been overcome, restricting the area where this approach is applied (Ahmadjian, 1973b, 1990).
Integrating the first group of approaches and methods used for obtaining plant tissue cultures, Y. Yamamoto (1985) founded another branch of experimental lichenology: cultivation of ground lichen thallus fragments on artificial growth media (Fig. 2). One of the most important advantages of this approach was using vegetative parts of the thallus, which made the approach equally applicable to lichens with different modes of reproduction. In addition, Yamamoto's method allowed obtaining, under controlled conditions and on a tight timetable, a biological system consisting of both components and displaying a number of properties typical of intact lichens (Yoshimura et al., 1993; Yamamoto et al., 1993). Taking into account the fact that under particular conditions for cultivating such systems, thalli morphologically and anatomically similar to the natural ones are formed (Yoshimura & Yamamoto, 1991; Yoshimura et al., 1993), this approach can be used for reproducing lichens
The interest towards these studies can be illustrated by the fact that the number of lichen species cultivated
4. Lichen resynthesis and producing artificial (model) associations
The method of lichen resynthesis includes the following stages: (1) dissociation of the natural lichen into components; (2) obtaining monocultures of the mycobiont and photobiont; (3) mixed cultivation of the mycobiont and photobiont; and (4) producing a stable morphogenetic association (Fig. 1).
4.1. Lichens used in attempts of resynthesis
Although works on the experimental resynthesis of lichens are in progress since over 70 years ago, the number of species of lichens resynthesized to date remains not too high (Table 1). Experiments were successful with far from all systems, and those lichen species that produced more or less developed thalli in resynthesis experiments, such as
Most attempts to reconstruct thalli were made on lichens with green algal photobiont (Fig. 4). Among lichens with cyanobacterial photobiont, successful resynthesis was achieved only in members of the genus
|Lichen name||Photobiont genera||Source|
|Acarospora fuscata||Trebouxia||Cited by: Оksner, 1974|
|Buelia spp.||Trebouxia||Cited by: Оksner, 1974|
|Bacidia bagliettoana||Chlorella||Bornet, 1873|
|Caloplaca decipiens||Chlorella||Cited by: Оksner, 1974|
|Cladonia cristatella||Trebouxia or Chlorella||Remmer, Ahmadjian, Livdahl, 1986 — cited by: Ahmadjian & Paracer, 1986 ; Ahmadjian, 1967|
|C. furcata||Trebouxia or Chlorella||Jahns, 1978 — cited by: Ahmadjian & Paracer, 1986|
|C. pyxidata||Trebouxia or Chlorella||Thomas, 1939 — cited by: Оksner, 1974|
|Collema limosum||Nostoc||Rees, 1871 — cited by: Оksner, 1974|
|Dermatocarpon miniatum||Hyalococcus or Protococcus||Stocker-Worgotter & Turk, 1989|
|Endocarpon pusillum||Myrmecia||Bertsch, Butin, 1967; Stahl, 1877 — цит по Stocker-Worgotter & Turk, 1988|
|Graphidaceae g. sp.||Trentepohlia||Hеrriset, 1946 — cited by: Оksner, 1974|
|Heppia echinulata||Scytonema, cyanobacterium||Marton, Galun, 1976 — cited by: Ahmadjian & Paracer, 1986|
|Huilia albocaerolescens||green alga||Cited by: Ahmadjian & Paracer, 1986|
|Lecanora spp.||Protococcus||Ahmadjian, Russell, Hildreth, 1980; Culberson, Ahmadjian, 1980 — cited by: Ahmadjian & Paracer, 1986|
|Lecidia spp.||Trebouxia, Pseudochlorella, Coccobotrys or Chlorosarcinopsis||Cited by: Ahmadjian & Paracer, 1986|
|Lepraria spp.||Stichococcus or Chlorella||Cited by: Ahmadjian & Paracer, 1986|
|Leptogium issatschenkovi||Nostoc||Danilov, 1929 — cited by: Оksner, 1974|
|Peltigeria canina||Nostoc||Ahmadjian, 1989|
|Physia stellaris||Trebouxia||Bonnier, 1888|
|Rhizoplaca chrysoleuca||Protococcus||Cited by: Ahmadjian & Paracer, 1986|
|Rinodina sophodes||green alga||Bonnier, 1888|
|Sarcogyna spp.||Myrmecia||Cited by: Ahmadjian & Paracer, 1986|
|Stereocaulon vulcani||Trebouxia + cyanobacterium||Cited by: Ahmadjian & Paracer, 1986|
|Staurothele rugulosa||Protococcus||Stahl, 1877 — cited by: Stocker-Worgotter & Turk, 1988|
|Usnea strigosa||green alga||Ahmadjian & Jacobs, 1983, Ahmadjian & Jacobs, 1985|
|Verrucaria macrostoma||Coccobotrys or Protococcus||Stocker-Worgotter & Turk, 1989|
|Xanthoria parietina||Trebouxia||Bornet, 1873; Bonnier, 1889 — cited by: Оksner, 1974|
The proportion of such studies performed on photobionts from the genus
Vegetative reproduction structures, soredia and isidia, are found only in three of the reconstructed lichen species. Experimental resynthesis requires a culture of the mycobiont, and Ahmajian (1973a) proposed using spores for extracting it from the naturally growing lichen. This is probably why lichens with mainly vegetative mode of reproduction were seldom used in such
It has been found that successful resynthesis of a lichen thallus from mycobiont and photobiont monocultures requires experimental conditions imitating the natural environment: the substrate and medium should be poor, so that the lichen does not dissociate into components, and neither the mycobiont or photobiont get any advantages for growth at any stage of development. To induce thallus differentiation in a mixed culture, alternating drying and moistening periods should be imposed, and the drying of biomass should be gradual (Ahmadjian, 1973b).
4.2. Producing artificial (model) associations
Work on the resynthesis of lichens and producing model association has laid the foundation for solving a number of basic problems of the current lichenology. One of these problems is the study of selectivity and specificity of symbiont interaction in lichens. For solving this problem in the course of lichen thallus resynthesis, it is possible to cultivate the mycobiont in pairs with free-living algae or photobionts in combinations not found in nature. In 50% of such experiments, the model systems revealed the lichenization of photobiont cells: the initial contact between the partners of the association took place, and pre-thalli were formed (Tables 2, 3). The same mycobiont showed both positive and negative results with algae of different species of the same genus or with photobionts extracted from the same lichen. The cause of this selective interaction of partners in pairs has not yet been revealed; however, several factors affecting the success of this process were determined, the most important of them being the source of the isolated photobiont and its symbiotrophics (Ahmadjian & Jacobs, 1983).
|Lichen from which mycobiont was extracted||Natural photobiont||Potential photobiont||Source of photobiont||Result|
|Cladonia cristatella||Trebouxia ereci||Friedmannia israeliensis||free-living||+|
|Myrmecia sp.||not specified||-|
|Pseudochlorella sp.||not specified||-|
|Pseudotrebouxia sp.||Xanthoria parietina||-|
|Trebouxia sp.||Pilophoron sp.||+|
|Trebouxia sp.||Stereocaulon sp.||+|
|Trebouxia sp.||Gymnoderma sp.||+|
|Trebouxia sp.||Lecidia sp.||+|
|Trebouxia sp.||Lepraria sp.||+|
|Trebouxia sp.||Parmelia sp.||-|
|Trebouxia sp.||Xanthoria aureola||-|
|Trebouxia italiana||Xanthoria parietina||+|
|Endocarpon pusillum||Myrmecia biatorellae||Protococcus staurothelis||Staurothele regulosa||-|
|Glyphis lepida||Trentepohlia||Trentepohlia sp.||Pyrenula nitidula||+|
|Phaeographina fulganata||Trentepohlia||Trentepohlia sp.||Pyrenula nitidula||+|
|Staurothele regulosa||Protococcus staurothelis||Myrmecia biatorellae||Endocarpon pusillum||-|
For instance, species of the genus
|Potential photobiont||Source of photobiont||Potential mycobiont||Result|
|Friedmannia israeliensis||free-living||Cladonia cristatella||+|
|Myrmecia sp.||not specified||Cladonia cristatella||-|
|Myrmecia biatorellae||Endocarpon pusillum||Staurothele regulosa||-|
|Nostoc sp.||cycads||Cladonia cristatella||-|
|Pleurastrum terrestre||free-living||Cladonia cristatella||-|
|Protococcus staurothelis||Staurothele regulosa||Endocarpon pusillum||-|
|Pseudochlorella sp.||not specified||Cladonia cristatella||-|
|Pseudotrebouxia sp.||Xanthoria parietina||Cladonia cristatella||-|
|Trebouxia sp.||Pilophoron sp.||Cladonia cristatella||+|
|Stereocaulon sp.||Cladonia cristatella||+|
|Gymnoderma sp.||Cladonia cristatella||+|
|Lecidia sp.||Cladonia cristatella||+|
|Lepraria sp.||Cladonia cristatella||+|
|Parmelia sp.||Cladonia cristatella||-|
|Xanthoria aureola||Cladonia cristatella||-|
|Trebouxia italiana||Xanthoria parietina||Cladonia cristatella||+|
|Trentepohlia sp.||Pyrenula nitidula||Glyphis lepida||+|
|Trentepohlia sp.||Pyrenula nitidula||Phaeographina fulganata||+|
On the other hand, photobionts isolated from lichen
Unfortunately, there are no data in the literature on model associations based on lichen photobionts and free-living fungi (Table 3). Meanwhile, we believe that this problem is very interesting, since the photobiont, intensely influenced by the mycobiont in the lichen, has developed a number of protective adaptations. These adaptations may be conductive to success in producing model associations of the photobiont and free-living symbiotic fungi.
Another problem is the construction of artificial three-component associations. For solving this problem, a third component, a cyanobiont, can be added to the resynthesized lichen to study the process of cephalodia formation (Ahmadjian, 1989; Ahmadjian & Jacobs, 1983; Ahmadjian & Paracer, 1986). Attempts to model three-component lichens have been made by adding strains of the symbiotrophic and free-living
A large field for work in experimental lichenology is opened by the opportunity to obtain protoplasts of the symbionts. The methodology of this procedure for a lichen mycobiont grown from a spore was developed by V. Ahmadjian (1991). Kinoshito (Yamamoto et al., 1993; Kinoshito et al., 2001) modified this procedure to develop a method independent of the way of mycobiont extraction, making it possible to obtain mycobiont protoplasts from vegetative parts of the thallus (experiments on obtaining mycobiont protoplasts were performed only with members of the genus
5. "Tissue cultures" of lichen
The field of experimental lichenology has been developed especially to obtain "callus tissue cultures" of lichens. Currently, lichens cultivated on solid growth media include 52 genera from 22 families, represented mainly by fruticose forms (52%), somewhat fewer foliose forms (36%), and only 12% crustose forms. The vast majority of the species maintained in "callus cultures" are two-component lichens with green algal photobionts. Attempts to introduce three-component lichens to a "callus culture" result in their dissociation into components and formation of "chimeric" forms (see below).
The ratio of different modes of reproduction among cultivated lichens differs from that among reconstructed ones, and there is no pronounced prevalence of forms with sexual or asexual reproduction (necessary for extracting the mycobiont according to the first group of experimental approaches). On the contrary, there is quite a high proportion of lichens with vegetative reproductive structures, soredia and isidia: 36% together, the same as the proportion of lichens with apothecia (37%). Furthermore, the universality of the approach allows cultivating fragments of lichens that form apothecia extremely rarely (Fig. 5B).
5.1. Problems emerging in the course of lichen cultivation
Among difficulties of this experimental approach, the high proportion of explants infected with accompanying microorganisms and the low proportion of thallus fragment germination can be named. In the case of
Yoshimura et al. (1993) note that attempts to cultivate fragments of the cyanolichen
One drawback of the method for obtaining lichen "tissue cultures" described in the literature is the total lack of any primary sterilization of explants. Although some studies refer to unsuccessful attempts to sterilize thallus fragments with mercuric chloride (Ahmadjian, 1989), our data (Smirnov & Lobakova, 2007) demonstrate the efficiency of consistent complex usage of "mild" sterilizing agents (such as hydrogen peroxide, alcohol, chlorhexidine).
In our opinion, works aimed by obtaining "suspension cell cultures" of lichens are especially promising. Experiments in this branch of lichenology are rare and fragmentary, while maintaining symbiont monocultures in liquid media are currently a common practice (Ahmadjian & Jacobs, 1983). In "suspension cultures" of lichens, thalli are not formed; this is why in most studies aimed at obtaining structured lichen thalli, even if thallus fragments were inoculated into liquid growth media, the experiments were never completed, because provisional results did not comply with the initial aims. On the other hand, obtaining "suspension cultures" of lichens is of considerable interest for biotechnology, since methods based on "suspension cultures" are easier to introduce into the industry. Thus, this mode of growth will probably allow obtaining large amounts of dedifferentiated cell biomass and using it in biotechnology as sources of lichen compounds.
A promising approach for obtaining both "suspension cultures" and "callus cultures" of lichens involves using "nurse cultures" (Smirnov & Lobakova, 2008) and conditioning (processing) the cultivating media with metabolites of associated organisms. Data available in the literature give evidence that the initiation of symbiont growth (especially of the mycobiont) requires compounds secreted by the photobiont or associated bacteria. It has been found that the development of the mycobiont was quicker and more intense if (1) non-sterile tree bark was used as the substrate; (2) the cultivating medium was conditioned by metabolites of the photobiont or bacteria; (3) the spores were infected by bacteria (Ahmadjian, 1989; Yolando et al., 2002; Smirnov, 2006). Our results show that conditioning the media with simple metabolites (after sterilization) is inefficient, compared to using native metabolites (dialysis cultivation).
5.2. Study of lichen morphogenesis
A special place among experimental works in lichenology is occupied by the branch involving the study of lichen thallus morphogenesis and revealing the factors influencing this process. A number of studies have addressed the problem of inducing morphogenesis in "callus cultures" of soredia, both in the laboratory, and in the natural environment (Stocker-Worgotter & Turk, 1988; Stocker-Worgotter & Turk, 1989; Yoshimura & Yamamoto, 1991; Armaleo, 1991; Yoshimura et al., 1993). Comparison of natural lichen thalli with those obtained by inducing morphogenesis in
Lichens with different modes of reproduction (sexual, asexual and vegetative) under laboratory conditions with morphogenesis induction undergo the same stages of development (lag phase, arachnoid phase, prethallus and thallus: Ahmadjian, 1973а, 1973b; Ahmadjian & Jacobs, 1983; Stocker-Worgotter & Turk, 1989; Yoshimura et al., 1993) as in nature (Ott, 1988). The only difference is the duration of particular stages, depending on the type of the explant and conditions of its cultivation. In
The experimental approaches in lichenology described here are currently used for solving a number of basic problems like those persistent in biotechnology. The former approaches include studying the ecological and morphological plasticity of lichens and revealing differentiation factors of thalli and the share of each partner in the formation of the unique super-organism system.
In this respect it is especially interesting to study the development of the "tissue cultures" of three-component lichens, such as
The phenomenon described provides an experimental confirmation of the idea that mycobionts can include several morphotypes (as analysis of their DNA has also shown), and/or the formation of chimeric lichens is possible. While the existence of such chimeras was earlier considered unproven, now the reality of this phenomenon has been confirmed both by some field studies (for review, see: Plyusnin, 2002) and by laboratory experiments.
Morphogenetic "tissue cultures" of lichens are convenient experimental models for the study of this phenomenon. The results of using them allow us to state that the formation of a particular morphotype or chimeric lichen depends on moisture. For instance, these results allow suggesting that the cyanobacterial morphotype is more widespread than has been believed earlier and unidentified species of the genus
It can be assumed that experimental approaches will also play an important role in the molecular biology of cyanolichens: they will allow studying the exchange of genes, inferred by some authors, between the symbionts by means of plasmids in the course of morphogenesis (Ahmadjian, 1991). Reviewing the data available in the literature has shown that for studying the early stages of lichen thallus morphogenesis, it is better to use methods of resynthesis, while for the study of specificity and selectivity of interactions between components of this symbiosis, as well as of different stages of thallus differentiation, the "tissue culture" and morphogenesis induction methods are more suitable.
Dedifferentiated mixed cellular aggregates of a "callus culture" of lichens can be used in the study of the genetic control over symbionts in the course of the formation of a balanced super-organism system (Yamamoto et al., 1993; Yoshimura et al., 1993).
5.3. The biotechnological potential of lichen "tissue cultures"
Using experimental approaches is promising also for producing from lichens their unique secondary metabolites, the lichen compounds. The biosynthesis of lichen compounds in "tissue cultures" is usually no different from that in the natural thallus in the composition of depsides, tridepsides, and depsidones; triterpenoid compounds are, however, a more labile class of substances, and in "callus cultures" of lichens they often disappear (Table 4).
In most cases, the concentration of lichen compounds in a "culture" is considerably lower than in a natural thallus: the content of the usnic acid in
|Class of compounds||Compounds||Usnea strigosa||Usnea rubescens||Ramalina yasudae||Peltigera pruinosa||Peltigera aphthosa|
The expediency of using lichen "tissue cultures" for obtaining biologically active compounds is also supported by the fact that their methanol and acetone extracts demonstrate a levels of superoxide dismutase activity, and have antibacterial (against Gram-positive bacteria: Fig. 6) and antiviral (when EBV test system is used: Fig. 7) effects (Yamamoto et al., 1993; Yamamoto et al., 1995).
The degrees of antibacterial and antiviral activities strongly vary between different lichens, even among species of the same genus (Fig. 7). In most cases, the inhibitory action of extracts of natural thalli is higher than that of "tissue culture" extracts; there are, however, some exceptions: laboratory extracts of
On the other hand, "tissue cultures" of lichens of the genera
Interestingly, the concentration of lichen compounds in reconstructed lichen thalli is often higher than in nature; Ahmadjian and Jacobs (1985) explain this by the more favourable conditions for lichen development formed in the course of resynthesis. It is noteworthy that producing artificial associations, with symbiont combinations not found in nature, can be used as a promising source of new antibiotic compounds. The possibility of this application is demonstrated by the two novel compounds, not typical of this species in nature, found in the thallus of
A special place among the problems of current lichenology is occupied by the conservation of rare lichen species and their re-introduction into the natural environment. The above-described experimental approaches can be used, among other purposes, for solving these problems. Methods of rare species gene pool conservation in collections and cryobanks are well-developed for higher plants (Street, 1977; Butenko, 1999). Some authors (Tolpysheva, 1998) believe that it would be useful to apply this experience to lichens as well.
Among experimental approaches in lichenology, two groups of methods can be recognized: lichen resynthesis and cultivation. The former approach helped to find the answers to many questions of lichen biology, but currently it faces a number of insoluble problems (e.g., the failure of attempts to produce mature spores in sporocarps), due to which the number of studies on lichen reconstruction has considerably decreased (Ahmadjian, 1990). The latter approach is promising for introducing lichens into the field of biotechnological developments. However, this is largely hindered by the low yield of lichen biomass in the course of cultivation. Two principal causes of this can be named: the considerable level of infection with fungi and bacteria (Yamamoto et. al, 2004) and the insufficiently quick growth of the culture of the lichen itself. The solution to the problem of "explant" infection with contaminant species may be found in surface sterilization of lichens, similar to that used in plant physiology (Smirnov & Lobakova, 2007). The solution to the problem of culture growth acceleration may be found in conditioning the media with secondary metabolites of various origins. The analysed literature contained no mentions of using "nurse cultures", a method widely used in plant physiology, considerably increasing the rate of growth in cultures (Street, 1977; Butenko, 1999; Butenko et al., 1987). At the same time, a number of authors have shown that secondary metabolites, both of associated fungi and algae, extracted from lichens (Vainshtein, 1988), and of accompanying fungi and algae (Ahmadjian, 1989), can accelerate growth in cultures of isolated symbionts, both mycobionts and phycobionts. Another way of accelerating the growth of cultures, both of the symbionts and of the lichen as a whole, may be found in using suspension cultures. Conditioning of media and suspension cultures can also be useful in the first group of experimental approaches, especially in producing model associations based on lichen photobionts (according to the literature, in most cases it was the mycobiont that served as the basis for novel associations).
The authors are grateful to Yu.T. Dyakov for the idea to write a paper on this subject, to A.K. Eskova for useful discussions and to P.N. Petrov for his invaluable help in the English text of the manuscript.
Ahmadjian V. Jacobs J. В. 1985Artificial reestablishment of lichens IV. Comparison between natural and synthetic thalli of Usnea strigosa. 17 149 165.
Ahmadjian V. Jacobs J. В. 1983Algal-fungal relationships in lichens: recognition, synthesis, and development.- In Goff. L. J., (Ed.): , 147 172.- Cambridge: Cambridge University Press.
Ahmadjian V. Paracer S. 1986. Clark University Press. 14 36.
Ahmadjian V. 1961Studies on lichenized fungi. 64 168 179.
Ahmadjian V. 1967. Blaisdell, Waltham, MA. 152 pp.
Ahmadjian V. 1973aMethods of isolation and culturing lichen symbionts and thalli ( 653 660). In: Ahmadjian V, Hale M. E. (eds) . Academic Press, New York.
Ahmadjian V. 1973bResynthesis of lichens, 565 579. In V. Ahmadjian & M. E. Hale (eds.), . New York and London.
Ahmadjian V. 1989Studies on the isolation and synthesis of bionts of the cyanolichen Peltigeria canina (Peltigeraceae). 165 29 38.
Ahmadjian V. 1990What have synthetic lichens told us about real lichens? 38 3 12.
Ahmadjian V. 1991Molecular biology of lichens: a look to the future. . 11: Р. 249 254.
Armaleo D. 1991Experimental microbiology of lichen. . 11: Р. 163 178.
Bertsch Butin 1967Die Kultur der Erdflechte Endocarpon pusillum im Labor. 72 29 42.
Bonnier G. 1888Germination des spores des lichens sur les protonemas des mousses et sur des algues differentes des gonidies du lichen. . Paris 40 541 543.
Bornet J.-B.-E. 1873Recherches sur les gonidies des Lichens. ., 5 с, V. XVII.
Bubrick P. Frensdorff A. Galun M. 1985Proteins from the lichen Xanthoria parietina (L.) Th. Fr. which bind to phycobiont cell walls: isolation and partial purification of an algal-binding protein.- 1 85 95.
Butenko R. G. 1999. Moscow: FBK-press, 159 p.
Butenko R. G. Gusev M. V. Kirkin A. F. Korzhenevskaya T. G. Makarova E. N. 1987. Book 3. Moscow: Vyshaya Shkola, 127 p.
Culberson C. F. 1969Chapel Hill: University of North Carolina, 628 p.
Czech H. 1927Kultur von pflanziichen Gevebezellen. . Bd. 3. S. 176 200.
Famintsyn A. S. 1907[On the role of symbiosis in the evolution of organisms] . Ser. 8. 20 3 15 39.
Galun M. 1989. M. Galun (ed.).- 1CRC Press Inc., Boca Raton, Florida, 1989.- 297 p.
Gautheret R. 1932Sur la culture d’extremites de racines. Compt. . t. 109, 1236 1238.
Gusev M. V. Mineeva L. A. 1992. Moscow: MSU, 448 p.
Harrison R. 1907Observation on the living developing nerve fiber. 4 140 143.
Kinoshito Y. Yamamoto Y. Kurokawa T. Yoshimura I. 2001Influence of nitrogen source on usnic acid production in a cultured mycobiont of lichen Usnea hirta (L.) Wigg. . 65 8 1900 1902.
Komine M. Iwasaki Y. Yamamoto Y. Hara K. 2004Developing a suitable growth substrate for lichen forced cultivation under an artificial environment. Lichens in focus- IAL 6.
Krasilnikov N. A. 1949[Lichen microflora.] . 18 3 3 24.
Manojlovic N. T. Solojuc S. Sukdolak S. 2002. 34N. 1. 83 85.
Mereschkowski K. S. 1907[.] Doct. Sci. Dissertation. Kazan: Kazan Imperial University. 402 pp.
Mereschkowski K. S. 1909[Theory of two plasms as the foundation of the symbiogenesis theory, a new doctrine on the origin of organisms] 7697 p.
Oksner A. N. 1974[Guide to the lichens of the USSR. 2.] Leningrad: Nauka, 283 p.
Ott S. 1988Photosymbiodemes and their development in Peltigera venosa. 20 361 368. Pl. Syst. Evol. 165: 29-38.
Paracer S. Ahmadjian V. 2000(Oxford Univ. Press, Oxford, 2nd ed.). 0-195-11806-5261 p.
Plyusnin S. N. 2002[Intrathallic variation of lichens] 53 15 16.
Prat S. 1927The toxity of tissue juices for cells of the tissue. 14: 121.
Rai A. N. 1990Cyanobacterial-fungal symbioses: the cyanolichens.- In Handbook of Symbiotic Cyanobacteria. Rai A.N. ed. 9 41.- CRS Press, Boca Raton: Florida. USA.
Smirnov I. A. Lobakova E. S. 2007[Peculiar features of lichen photobiont cultivation] Materials of All-Russia Conference with International Participation. Saratov: Nauchnaya Kniga. 32
Smirnov I. A. Lobakova E. S. 2008[Morphophysiological description of a mixed cultures of Pleurotus ostreatus and the nitrogen-fixing cyanobacteria Anabaena variabilis] . Moscow: Vostok-Zapad. 198 199.
Smirnov I. A. 2006[Micromycetes associated with Cetraria islandica] Moscow: MAKS press. 211
Stocker-Worgotter E. Turk R. 1988Culture of the cyanobacterial lichen from soredia under laboratory conditions. 20 369 375.
Stocker-Worgotter E. Turk R. 1989Artificial cultures of lichen Peltigera didactyla in natural environment. 165 39 48.
Street H. E. 1977. Botanical Monographs. 11. Blackwell Scientific Publications, Oxford, London, Edinburg, Melbourne.
Tolpysheva T. Yu. 1984a[Effect of extracts from lichens on fungi. 1. Effect of water extracts of Cladina stellaris and C. rangiferina on the growth of soil fungi] . Т. 18. 4 287 293.
Tolpysheva T. Yu. 1984b[Effect of extracts from lichens on fungi. 2. Effect of integrated preparations from Cladina stellaris and C. rangiferina on the growth of soil fungi] . 18 5 384 388.
Tolpysheva T. Yu. 1985[Effect of extracts from lichens on fungi. 3. Effect of usnic acid and atranorin on the growth of soil fungi] .. 19 6 482 489.
Tolpysheva T. Yu. 1998 Moscow: Argus; Russky Universitet. 501 514.
Vainshtein E. A. Tolpysheva T. Yu. 1992[Effect of extract from the lichen Hypogymnia physodes (L.) Nyl. and of pure lichen acids on wood-rotting fungi] . 26 6 448 455.
Vainshtein E. A. 1982a. P. 1. Leningrad: Deposited in VINITI, nos. 210-83. p. 1-238.
Vainshtein E. A. 1982b. P. 2. Leningrad: Deposited in VINITI, nos. 210-83. p. 239-485.
Vainshtein E. A. 1982c. P. 3. Leningrad: Deposited in VINITI, nos. 210-83. p. 486-717.
Vainshtein E. A. 1988[.] Extended Abstract of Doct. Sci. Dissertation. Leningrad., 45 p.
Vochting H. 1892. Tubingen.
White Ph. 1932Influence of some environmental condition on the growth of excised root tips of wheat seedlings of liquid media. . 4 613 628.
Wolf Е. Schii.ler А. 2005Phycobiliprotein fluorescence of Nostoc punctiforme changes during the cycle and chromatic adaptation: characterization Ьу spectral CLSM and spectral unmixing . 2Р. 480-491.
Yamamoto Y. Miura Y. Higuchi M. Kinoshita Y. Yoshimura I. 1993Using lichen tissue cultures in modem biology. 96: 384 393.
Yamamoto Y. Miura Y. Kinoshita Y. Higuchi M. Yamada Y. Muracami A. Ohigashi H. Koshimizu K. 1995Screening of tissue cultures and thalli of their active constituents for inhibition of tumor promoter-induced Epstein-bar virus activation. . 43 (8), 1388- 1390.
Yamamoto Y. Mizuguchi R. Yamada Y. 1985Tissue cultures of Usnea rubescens and Ramalina yasudae and production of usnic acid in their cultures. 49 3347 3348.
Yamamoto Y. Takeda M. Hara K. Komine M. Inamoto T. Kawakatsu M. Miyagawa H. 2004Screening for antibacterial activities and isolation of antibiotics from mycobiont cultures. .
Yolando et al. 2002Bioprodaction of lichens phenolics by immobilized lichen cels with emphasis on the role of epiphytic bacteria. №92, 245 260
Yoshimura I. Yamamoto Y. 1991Development of Peltigera praetextata lichen thalli in culture. . 11: 109 117.
Yoshimura I. Kurokawa T. Yamamoto Y. Kinoshita Y. 1993Development of lichen thalli in vitro. 96 412 421.