Generalized anxiety, panic, and posttraumatic stress disorder (PTSD) are debilitating conditions, which have an incidence of one in ten persons in the general population and epidemiological studies also report that these disorders often occur with depression (1-3). Anxiolytic benzodiazepines, including diazepam and alprazolam, remain the best and most used treatments for these conditions (4-7). However, their therapeutic use is associated with side effects, which include sedation and rapid development of tolerance as well as dependence. This results in severe discontinuation symptoms and often to drug abuse (4-6, 8; 9).
In many patients, including patients with PTSD, the pharmacological effects of these drugs are very weak and there is a large number of non-responders (10-12). This has stimulated drug design that for many decades has focused in the development of new more effective therapies for anxiety disorders (13-15). Novel neuronal biomarkers for the pharmacological targets of the next generation of anxiolytic drugs have been discovered.
The downregulation of neurosteroid biosynthesis has been implicated in the pathophysiology of anxiety and depressive disorders (reviewed in 16). Decreases in cerebrospinal fluid (clinical studies) and brain content (preclinical studies) of the GABAA receptor-active progesterone derivative, allopregnanolone, have been associated with affective and mood disorders, which includes depression, anxiety spectrum disorders, PTSD, premenstrual dysphoric disorder, schizophrenia, and impulsivity (17-27). Thus, elevating or normalizing the downregulation of brain allopregnanolone levels could be a promising therapeutic strategy for these psychiatric disorders. This prompted investigations to develop new neurosteroidogenic agents to contrast allopregnanolone biosynthesis deficits in anxiety and depression (28-31).
We measured allopregnanolone levels in the cerebrospinal fluid (CSF) of PTSD patients assuming that allopregnanolone levels in the CSF reflect the levels of this neurosteroid in the brain (17). Also, in depressed patients, the concentration of allopregnanolone in the CSF was decreased by about 50-60% of the levels measured in non-psychiatric patients (26). The CSF allopregnanolone level decrease is likely induced by a downregulation of the expression of 5α-reductase type I mRNA in the prefrontal-cortex (area BA9) that we measured in depressed patients and age- and sex-matched non-psychiatric subjects (32). The cortical level of 5α-reductase mRNA in depressed patients was dramatically decreased to about 50% of the levels measured in non-psychiatric comparison subjects, whereas the levels of 5α-reductase mRNA was unchanged in the cerebellum (32). In depressed patients, SSRI treatment with fluoxetine and fluvoxamine normalized the CSF allopregnanolone content (26) in a manner that correlated with the improvement in depressive symptoms. These results were confirmed in studies that determined allopregnanolone or levels of 5α-tetrahydrodeoxycorticosterone, another positive modulator of GABAA receptor function, in the plasma of depressed patients treated with SSRIs (33).
In premenopausal women with PTSD, the CSF allopregnanolone levels were decreased by about 60% and were inversely correlated with PTSD re-experiencing and comorbid depressive symptoms (17). Interestingly, CSF allopregnanolone levels were lowest in those patients with PTSD and comorbid depression. Also, the
Following the finding that fluoxetine and paroxetine and other SSRIs increase the content of allopregnanolone in several rodent brain structures (34), we hypothesized that normalization of brain allopregnanolone levels may underlie the pharmacological effects of the so called “selective serotonin reuptake inhibitors” or SSRIs in mood disorders. To test this hypothesis, we conducted experiments using the socially isolated mouse as an animal model of anxiety disorders and PTSD (16; 35-38). The socially isolated mouse expresses a robust decrease of corticolimbic allopregnanolone levels, which are associated with anxiety-like behaviors, fear, resistance to sedation, and heightened aggression (16; 35; 39). These behavioral deficits can be ameliorated by administration of fluoxetine and other SSRIs that upregulate allopregnanolone levels. Interestingly, fluoxetine’s pharmacological effects resulted to be independent from the ability of this drug to inhibit serotonin reuptake (35; 36).
Our experiments support a selective and novel mechanism whereby SSRIs, acting as selective brain
2. Neurosteroids modulation of GABAA receptors function
Biosynthesis of neurosteroids in the brain is independent from adrenals, ovaries, and testis (40-44). Neurosteroids are functionally active in modulating gene expression and neurotransmitter systems (45-52). Allopregnanolone exerts pharmacological actions, such as anticonvulsant, anxiolytic, antidepressant, and even sedative-hypnotic (53-60). These pharmacological actions are similar to those elicited by barbiturates and benzodiazepines (52; 61; 62). Allopregnanolone potently (nM affinity), positively, and allosterically modulates the action of GABA at GABAA receptors (45-51). The endogenous physiological relevance of allopregnanolone is substantiated by its facilitation and fine-tuning of the efficacy of direct GABAA receptor activators and positive allosteric modulators of GABA action at GABAA receptors (43; 47; 48; 63). The demonstration that allopregnanolone potentiates GABA responses via two binding sites in the GABAA receptor that, respectively, mediate the potentiation and the direct activation of the GABAA receptor by allopregnanolone has been pivotal in neurosteroid pharmacology (64). Also, GABAA receptors incorporating α4, α6, and δ subunits in combination with γ and β subunits show higher affinity (nM range) for allopregnanolone (45; 46; 51; 64; 65). Relevant for pharmacological strategies to overcome behavioral deficits resulting from GABAA receptor signal transduction deficits, allopregnanolone allosteric modulation of the action of GABA at GABAA receptors is much less selective than that of benzodiazepines, which are relatively inactive at α4- or α6-containing GABAA receptors (4; 45; 46; 66).
3. Neurosteroid biosynthesis in corticolimbic neurons
A study of the neuronal localization of the neurosteroidogenic enzymes, 5α-reductase type I and 3α-hydroxysteroid dehydrogenase (3α-HSD), has recently showed that these enzymes are not expressed in GABAergic cortical interneurons or glial cells (67). Of note, 5α-reductase and 3α-HSD were highly expressed and co-localized in a region-specific way in primary GABAergic and glutamatergic neurons, including pyramidal neurons, granular cells, reticulo-thalamic neurons, medium spiny neurons of the striatum and nucleus accumbens, and Purkinje cells in the cerebellum (67). This suggested that allopregnanolone synthesized in glutamatergic cortical or hippocampal pyramidal neurons or in granular cells of the dentate gyrus may be secreted in: 1) a paracrine manner which would allow allopregnanolone to reach GABAA receptors located in the synaptic membranes of other cortical or hippocampal pyramidal neurons, or 2) an autocrine fashion which would allow allopregnanolone to act locally by binding post-synaptic or extra-synaptic GABAA receptors located on the same dendrites or cell bodies of the cortical or hippocampal pyramidal neuron in which it was produced (67). Alternatively, allopregnanolone might not be released, but may instead diffuse laterally into synaptosome membranes of the cell bodies or dendritic arborization of glutamatergic neurons in which it is produced to attain intracellular access to specific neurosteroid binding sites of GABAA receptors (67; 68). In the amygdala, for example, this would functionally baffle the effects of concomitant excitatory inputs to glutamatergic projection neurons during exposure to unconditioned stress during fear conditioning or to conditioned stressors during extinction.
On the other hand, allopregnanolone produced in primary output GABAergic neurons from the reticular thalamic nucleus may secrete allopregnanolone simultaneously with GABA to concomitantly act at post-synaptic GABAA receptors inserted in glutamatergic thalamocortical neurons (69). Very similarly, allopregnanolone synthesized by striatal medium spiny GABAergic neurons and cerebellar Purkinje cells may activate post-synaptic GABAA receptors located on cell bodies or dendrites of neurons in the deep cerebellar nuclei (67).
The clarification of allopregnanolone site of synthesis and action across several brain regions has been pivotal to our understanding of the possible mechanisms by which allopregnanolone is secreted and acts at GABAA receptors. These studies underscore the functional role of allopregnanolone in fine tuning the strength of GABAergic neurotransmission under physiological conditions and how deficits in allopregnanolone biosynthesis may result in abnormal behavior.
4. Social isolation induces a selective neuron-specific decrease of 5α-reductase in corticolimbic neurons
Exposure of rodents to protracted social isolation stress for 4-8 weeks induces a decrease in allopregnanolone biosynthesis in several corticolimbic structures as a result of a downregulation of the mRNA and protein expression of 5α-reductase type I (35; 70-73; reviewed in 38). Socially isolated mice show a 70% reduction in the synthesis rate of allopregnanolone and 5α-DHP biosynthesis compared to group-housed mice (35; 72).
Allopregnanolone and 5α-DHP are unevenly distributed and expressed in various brain structures (48; 74). The rodent olfactory bulb shows the highest concentrations of 5α-DHP and allopregnanolone followed by the frontal cortex, hippocampus, amygdala, striatum, and cerebellum (74). Interestingly, the largest decrease of 5α-reductase was found in brain regions regulating emotional behavior, including the amygdala and hippocampus, followed by the olfactory bulb and the frontal cortex (74). The expression of 5α-reductase failed to change in the cerebellum and striatum (74; 75). Decreased 5α-reductase was specifically found in cortical pyramidal neurons of layers V-VI, in hippocampal CA3 pyramidal neurons and glutamatergic granular cells of the dentate gyrus, and in the pyramidal-like neurons of the basolateral amygdala (75). However, 5α-reductase fails to change in GABAergic neurons of the reticular thalamic nucleus, central amygdala, cerebellum, and in the medium spiny neurons of the caudatus and putamen (75). In these brain areas, we confirmed that the decrease of 5α-reductase resulted in a reduction of allopregnanolone levels (74; 76; 77). Social isolation failed to change the expression of 3α-HSD, the mRNA expression of diazepam binding inhibitor, and the expression of the 18 kDa translocase protein (TSPO), which is involved in the transport of cholesterol across the inner mitochondrial membrane and activation of neurosteroidogenesis (reviewed in 72). Thus, the downregulation of 5α-reductase appears to be the main factor responsible for the reduction of corticolimbic allopregnanolone levels.
5. GABAergic neurotransmission deficits resulting from allopregnanolone downregulation
Allopregnanolone biosynthesis downregulation as a result of social isolation stress or pharmacological decrease of allopregnanolone induced by inhibiting 5α-reductase with the potent competitive 5α-reductase inhibitor SKF 105,111 decreases GABAergic neurotransmission as demonstrated by reduced loss of righting reflexes induced by GABAA receptor active ligands. The effects of SKF on the muscimol-, pentobarbital-, benzodiazepine-, or alcohol-induced loss of righting reflex loss can be reversed by the systemic or intracerebroventricular administration of allopregnanolone (43; 48). Likewise, social isolation or SKF-induced decrease of allopregnanolone results in facilitation of the seizure activity induced by several drugs that decrease GABAA receptor function, including picrotoxin (63). Administration of allopregnanolone at doses that have virtually no effects on group-housed control mice normalized the increased susceptibility to picrotoxin-induced seizures in SKF-treated or social isolated mice (63). The protracted social isolation or SKF treatment-induced allopregnanolone biosynthesis downregulation appeared to be the primary reason for the GABAA receptor signal transduction deficits observed in these mice. In fact, seizures induced by kainic acid or strychnine in socially isolated mice are similar to those induced by these agents in group housed mice.
6. Behavioral effects induced by allopregnanolone downregulation in corticolimbic areas
The decrease of allopregnanolone biosynthesis in socially isolated mice has been associated with several behavioral deficits that resemble behavioral abnormalities observed in patients with PTSD (16; 17; 30; 38). Hence, this mouse model can be used to study the behavioral responses elicited by treatment with neurosteroidogenic agents, the SBSSs. This new class of drugs includes the SSRI antidepressants that have been shown to elicit a potent neurosteroidogenic activity selectively at low doses as their principal action.
Allopregnanolone has emerged as an important biomarker of emotional behavioral deficits (16; 35-38; 72). This was demonstrated by experiments using socially isolated mice to induce a downregulation of allopregnanolone biosynthesis. We have established a fundamental role for allopregnanolone in the regulation of anxiety-like and aggressive behavior as well as contextual fear conditioning, (16; 37; 63; 74; 77). When mice are socially isolated for a period varying from one to eight weeks, there is a time-dependent increase in aggressive behavior over the first four weeks of isolation, which is inversely correlated with a time-dependent decrease of corticolimbic allopregnanolone levels (35). Likewise, socially isolated mice exposed to a classical fear conditioning paradigm showed enhanced conditioned contextual but not cued fear responses compared with group housed mice (74). The time-related increase of contextual fear responses correlated with the downregulation of 5α-reductase mRNA and protein expression observed in the frontal cortex, hippocampus, and amygdala (74). Socially isolated mice also exhibited impaired and incomplete fear extinction (74). Of note, socially isolated mice also exhibit higher levels of anxiety-like behavior, determined by the elevated plus maze and in the open field (16; 39).
Allopregnanolone plays a
7. Social isolation induces changes in GABAA receptor subunit expression
Postmortem studies suggest that altered corticolimbic GABAergic neurotransmission, GABA receptor binding and receptor subunit composition, as well as GABA synthesis and transport may be associated with various psychiatric disorders, including anxiety disorders, schizophrenia, and depression (85-88).
The regional distribution of GABAA receptor subunit subtypes plays an important role in the pharmacology of GABAA receptor ligands that bind to selective and specific GABAA receptor subunits (89-90). Recent studies showed that α1-containing GABAA receptors mediate the sedative properties of specific GABAergic ligands, such as diazepam, in the same way α2 and probably α3 subunits mediate the anxiolytic effects of benzodiazepines, and α5 subunits appear to be involved in learning and cognition (89; 90). High affinity binding of benzodiazepine to GABAA receptors requires the interaction of α and γ subunits (89; 90).
In socially isolated mice, we found changes in the mRNA and protein expression of several GABAA receptor subunits in the frontal cortex and hippocampus (91). The mRNA levels encoding α1, α2, and γ2 GABAA receptor subunit subtypes were reduced (~50%), while the mRNAs encoding α4 and α5 subunits were increased (~130%) compared to levels measured in group-housed mice (91). Protein levels of α1 and α5 determined in synaptic membrane preparations in the frontal cortex and hippocampus confirmed the former results. Using a laser microdissection technique coupled with nested RT-PCR amplification, we found that α1 mRNA levels were decreased by 50% in layer I neuropil, whereas the expression of α1 subunit mRNA in the pyramidal neurons of layer V was unchanged as a result of social isolation. Thus, changes in GABAA receptor subunits within one brain area are region-specific (91).
Changes in GABAA receptor subunit subtype composition are expected to result in altered pharmacological responses to various GABAA receptor ligands in socially isolated mice. As expected, socially isolated mice showed resistance to the sedative and anxiolytic properties of diazepam and zolpidem, positive allosteric GABAA receptor modulators that bind with high affinity to α1, α2, α3 or α5 subunit-containing GABAA receptors (diazepam) and to α1 subunit-containing GABAA receptors (zolpidem) (91). The α1 subunit of the GABAA receptor plays a primary role in mediating the sedative pharmacological effects of diazepam and zolpidem (92). Hence, their altered pharmacological response could result by a decrease in α1 subunit-containing GABAA receptors. Likewise, a decreased γ2 subunits support the formation of GABAA receptors in which this subunit might be substituted. Given that γ2 subunits are a necessary prerequisite for the formation of benzodiazepine-sensitive GABAA receptors (89; 90), the lack of anxiolytic activity of diazepam may result from the formation of benzodiazepine-insensitive GABAA receptors in neuronal circuits that regulate anxiety (39; 91).
Increases of 4 subunit-containing GABAA receptor expression in the frontal cortex appeared to be irrelevant to the behavioral or pharmacological alterations observed in socially isolated mice. GABA agonists such as THIP or the allosteric modulator, allopregnanolone, show selectivity and increased potency, respectively, for GABAA receptors containing 4/-subunits. These compounds comparably decrease locomotor activity in group-housed and socially isolated mice (91). In contrast to diazepam, allopregnanolone dose-dependently induces potent anxiolytic actions in socially isolated mice (16; 39).
Interestingly, the expression of GABAA receptor subunits is susceptible to changes in brain neurosteroid levels. In particular, expression of α4−containing subunits increases during progesterone withdrawal or following blockade of 5α−reductase (93). Likewise, in socially isolated mice, allopregnanolone levels decrease in several corticolimbic structures that concomitantly show changes in GABAA receptor subunit mRNA and protein expression. It would be important to determine whether social isolation directly affects the expression of GABAA receptor subunit composition or whether such changes are mediated by decreasing the levels of 5α−DHP and its binding at nuclear progesterone receptors or by allopregnanolone biosynthesis downregulation.
8. Selective brain steroidogenic stimulants (SBSSs) improve behavioral deficits in socially isolated mice
Behavioral deficits induced by social isolation in rodents include aggressive behavior (94-96). Aggression is correlated with the downregulation of corticolimbic allopregnanolone biosynthesis (35). Upregulation of allopregnanolone levels in socially isolated mice by systemic administration or local microinfusion of allopregnanolone induces a dose-dependent amelioration of aggressive behavior of a resident mouse to a same-sex intruder (35; 77). Thus, the decrease of corticolimbic allopregnanolone levels appears to be involved in the expression of aggression.
As indicated above, SSRI antidepressants potently increase the levels of allopregnanolone in rodents and depressed humans. The effects of paroxetine and fluoxetine on allopregnanolone levels were independent from pregnenolone or progesterone levels that failed to change (34; 76). Racemic fluoxetine (R- and S-isomers) normalized the righting reflex loss induced by pentobarbital in mice by increasing corticolimbic allopregnanolone levels (35-37). Of note, at the doses used, fluoxetine failed to change the behavior and allopregnanolone levels of group housed mice (35; 36). Importantly, inhibition of serotonin synthesis by treatment with p-chlorophenylalanine failed to block the behavioral effects of fluoxetine, suggesting that increasing corticolimbic allopregnanolone levels is part of the pharmacological actions of fluoxetine (76).
These observations led us to hypothesize that fluoxetine could improve the behavioral abnormalities of socially isolated mice by enhancing corticolimbic allopregnanolone biosynthesis rather than by inhibiting serotonin reuptake. This hypothesis was investigated using the R- and S-stereoisomers of fluoxetine and norfluoxetine as pharmacological tools. We expected that these drugs would stereospecifically upregulate corticolimbic allopregnanolone content but have no stereoselectivity with regard to inhibition of 5-HT reuptake. We additionally thought that doses of fluoxetine and norfluoxetine stereoisomers that increase corticolimbic allopregnanolone content might differ from those that inhibit 5-HT reuptake. Indeed (16; 35-38), fluoxetine dose-dependently and stereospecifically normalized the duration of pentobarbital-induced sedation and reduced aggressiveness, fear responses, and anxiety-like behavior at the same submicromolar doses that normalized the downregulation of brain allopregnanolone content in socially isolated mice. Interestingly, the S-stereoisomers of fluoxetine or norfluoxetine appeared to be 3 to 7 fold more potent than their respective R-stereoisomers and S-norfluoxetine was about 5-fold more potent than S-fluoxetine. Importantly, the effective concentrations (EC50s) of S-fluoxetine and S-norfluoxetine that normalize the brain allopregnanolone content are 10- (S-fluoxetine) and 50-fold (S-norfluoxetine) lower than their respective EC50s needed to inhibit 5-HT reuptake (35-38). Remarkably, the SSRI activity of S or R-fluoxetine and of S or R-norfluoxetine was devoid of stereospecificity (35; 36). Hence, this study demonstrated that neither the behavioral action nor the normalization of corticolimbic allopregnanolone content by S-fluoxetine and S-norfluoxetine is related to their intrinsic SSRI activity.
9. A novel promising therapy for anxiety disorders and PTSD
In the pathophysiology of depression and PTSD, a GABAergic neurotransmission dysfunction could at least in part be involved in the symptomatology of these disorders. Decreased GABA levels and reductions in GABAA and GABAB receptor binding and/or sensitivity have been found in depressed patients (97; 98). In PTSD, decreased frontal lobe benzodiazepine receptor binding (99; 100) and decreased plasma GABA levels (101) have been demonstrated. These changes were most consistently and profoundly observed among treatment resistant patients. Benzodiazepines have not been found to effectively treat PTSD (10-12) and SSRIs sertraline and paroxetine are the only medications currently approved by the Federal Drug Administration (FDA) for the treatment of PTSD. However, their effect sizes are modest (102-105), or even ineffective (106). In patients who cannot adequately synthesize allopregnanolone and in whom administration of an SSRI (or SBSS) is ineffective, the administration of an allopregnanolone analog (e.g. 107, 108), such as ganaxolone may offer a therapeutic alternative. A multisite Phase II trial of the efficacy and safety of ganaxolone in PTSD is currently been tested. Other medications that increase plasma allopregnanolone levels by a different mechanisms than the SSRIs also may be effective in PTSD (109-111).
The findings that the socially isolated mouse expresses decreased levels of allopregnanolone, as well as changes in the expression of several GABAA receptor subunits in corticolimbic structures that regulate cognition, anxiety, PTSD, and depression suggests that the
Hence, as in PTSD patients, the socially isolated mouse fails to respond to sedative and anxiolytic benzodiazepines. Our studies demonstrate that allopregnanolone or S-norfluoxetine -at nonserotonergic doses- infused into the basolateral amygdala potently increase allopregnanolone biosynthesis in target corticolimbic areas including the hippocampus, basolateral amygdala, and frontal cortex (77) and exert a strong anti-anxiety, anti-fear, and anti-aggression effect (35-38; 72; 77).
Neurosteroids lack GABAA receptor subunit selectivity and the functional GABAA receptor binding characteristics of benzodiazepines. Thus, this suggests that allopregnanolone, its analogs, or molecules that stimulate allopregnanolone biosynthesis might be advantageous over benzodiazepines in a scenario of neurosteroid downregulation and changes in GABAA receptor subunit subtypes. Despite benzodiazepines, allopregnanolone activates GABAA receptors incorporating α4, α6, and δ subunits in combination with γ and β subunits (64-66). Thus, allopregnanolone or SBSSs improve anxiety, fear, and aggressiveness when benzodiazepines fail. Of note and in contrast to benzodiazepines, both allopregnanolone and SBSS molecules decrease anxiety, fear, and aggression at concentrations that fail to be sedative (16; 35; 39; 77).
New SBSS molecules that fail to exert any significant SSRI activity but increase corticolimbic allopregnanolone levels and thereby improve behavioral symptoms in mouse models of anxiety and depression. The high potency and stereospecificity of these drugs in reducing behavioral deficits and in normalizing brain allopregnanolone content suggest that they may affect specific targets for regulating neurosteroidogenesis. The finding that protracted social isolation affects the expression of 5α-reductase in corticolimbic structures, but fails to change the expression of 3α-HSD, as well as the finding that brain progesterone levels don’t change in socially isolated mice suggest that a mechanism involving 5α-reductase is responsible for the decrease of corticolimbic allopregnanolone content. This is further supported by the fact that 5α-reductase is the rate-limiting step-enzyme in allopregnanolone biosynthesis from progesterone (73). Hence, these data suggest that fluoxetine and norfluoxetine mediate upregulation of corticolimbic allopregnanolone levels by a direct action on 5α-reductase. However,
Other feasible pharmacological targets to enhance neurosteroidogenesis include the translocase protein (18 kDa) or TSPO, previously called mitochondrial peripheral benzodiazepine receptor or PBR (114). TSPO represents the starting point and an important rate-limiting step in neurosteroidogenesis. It gives access to neurosteroids in the brain by regulating the entry of cholesterol into the inner mitochondrial membranes and its conversion to pregnenolone by P450scc, which is located in the inner mitochondrial membrane (29; 114). A cascade of enzymatic processes then take place in the cytosol, resulting in the production of neuroactive steroids, including pregnenolone sulfate, DHEAS (though apparently not in human brain (115)], THDOC, and allopregnanolone (reviewed in 31).
New molecules that bind with high affinity to TSPO have been recently investigated. These drugs are able to exert important anxiolytic effects but are devoid of the unwanted side effects associated with benzodiazepines, including over-sedation and tolerance (28; 29). In mouse models, TSPO agents have been shown to potently increase pregnenolone levels in the hippocampus and cortex, as well as to induce anxiolytic effects (116-119). TSPO ligands include XBD173 and etifoxine, which have proven to be highly efficacious anxiolytic and antidepressant drugs in a number of behavioral tests (29; 30). The anxiolytic and antidepressant effects of these agents were related their ability to increase neurosteroid biosynthesis, as confirmed by studies in which key enzyme blockers for neurosteroid biosynthesis, including finansteride and trilostane (56; 30), were used. TSPO ligands have recently showed promising therapeutic effects in clinical studies (29; 30).
10. Closing remarks
The new class of drugs, the SBSSs (selective brain steroidogenic stimulants) -whose mechanism of action involves the stimulation of neurosteroidogenesis with the goal of increasing brain allopregnanolone levels- has emerged as a new therapeutic strategy for the treatment of psychiatric disorders associated with a downregulation of brain allopregnanolone biosynthesis. These disorders include anxiety, depression, and PTSD.
In comparison to benzodiazepines, the SBSSs are more efficacious as well as devoid of the unwanted side-effects induced by benzodiazepines. Allopregnanolone pharmacology involves the allosteric modulation of GABA action at GABAA receptors, which is broader than that of benzodiazepines, which fail to modulate GABAA receptors containing α4 and α6 subunits. Hence, selective stimulation of allopregnanolone biosynthesis may avoid the therapeutic hindrances caused by the formation of benzodiazepine-resistant GABAA receptors with altered subunit composition, such as may occur in stress-related psychiatric disorders (reviewed 6in 120). Thus, novel SBSS drugs that specifically increase corticolimbic allopregnanolone biosynthesis appear to be a novel promising pharmacological class of future drugs for the treatment of anxiety disorders, depression, and PTSD.
This review was supported by the National Institute of Mental Health Grant MH 085999 (to G. Pinna).
Diagnostic Statistical Manual of Mental Disorders. 2000Washington DC: American Psychiatric Press;
Berton O. Nestler I. J. 2006New approaches to antidepressant drug discovery: beyond monoamines. ; 7 137 151
Kessler R. C. Berglund P. Demler O. Jin R. Merikangas K. R. Walters E. E. 2005Lifetime prevalence and age-of-onset distributions of DSM-IV disorders in the National Comorbidity Survey Replication. ; 62 593 602
Costa E. Guidotti A. 1996Benzodiazepines on trial: a research strategy for their rehabilitation. ; 17 192 200
Bandelow B. Zohar J. Hollander E. Kasper S. Möller H. J. et al. 2008World Federation of Societies of Biological Psychiatry (WFSBP) guidelines for the pharmacological treatment of anxiety, obsessive-compulsive and post-traumatic stress disorders- first revision. ; 9 248 312
Baldwin D. S. Anderson I. M. Nutt D. J. Bandelow B. Bond A. Davidson J. R. den J. A. Boer N. A. Fineberg M. Knapp J. Scott H. U. Wittchen 2005British Association for Psychopharmacology. Evidence-based guidelines for the pharmacological treatment of anxiety disorders: recommendations from the British Association for Psychopharmacology. ; 19 567 96
Rudolph U. Möhler H. 2006GABA-based therapeutic approaches: GABAA receptor subtype functions. ; 6 18 23
Nutt D. J. Ballenger J. C. Sheehan D. Wittchen H. U. 2002Generalized anxiety disorder: comorbidity, comparative biology and treatment. ; 5 315 25
Pinna G. Galici R. Schneider H. H. Stephens D. N. Turski L. 1997Alprazolam dependence prevented by substituting with the beta-carboline abecarnil. ; 94 2719 23
Viola J. Ditzler T. Batzer W. Harazin J. Adams D. Lettich L. Berigan T. 1997Pharmacological management of post-traumatic stress disorder: clinical summary of a five-year retrospective study, ; 162 616 9
Davidson J. R. 2004Use of benzodiazepines in social anxiety disorder, generalized anxiety disorder, and posttraumatic stress disorder. ; 65 29 33
Gelpin E. Bonne O. Peri T. Brandes D. Shalev A. Y. 1996Treatment of recent trauma survivors with benzodiazepines: a prospective study. ; 57 390 4
Atack J. R. 2003Anxioselective compounds acting at the GABA(A) receptor benzodiazepine binding site. ; 2 213 32
Costa E. 1998From GABAA receptor diversity emerges a unified vision of GABAergic inhibition. ; 38 321 50
Puia G. 1992Molecular mechanisms of the partial allosteric modulatory effects of bretazenil at gamma-aminobutyric acid type A receptor. ; 89 3620 4
Pinna G. Costa E. Guidotti A. 2006Fluoxetine and norfluoxetine stereospecifically and selectively increase brain neurosteroid content at doses that are inactive on 5-HT reuptake. ; 186 362 372
Rasmusson A. M. Pinna G. Paliwal P. Weisman D. Gottschalk C. Charney D. et al. 2006Decreased cerebrospinal fluid allopregnanolone levels in women with posttraumatic stress disorder. ; 60 704 13
Nappi R.E. Petraglia F. Luisi S. Polatti F. Farina C. Genazzani A.R. 2001. Serum allopregnanolone in women with postpartum “blues”. Obstet Gynecol ; 97: 77-80.
Ströhle A. Romeo E. di Michele F. Pasini A. Yassouridis A. Holsboer F. Rupprecht R. 2002. GABAA receptor-modulationg neuroactive steroid composition in patients with panic disorder before and during paroxetine treatment. Am J Psychiatry ; 159: 145-7.
Rapkin A. J. Morgan M. Goldman L. Brann D. W. Simone D. Mahesh V. B. 1997Progesterone metabolite allopregnanolone in women with presmenstrual syndrome. ; 90 709 14
34 1885 903 21. C.E. Marx, R.S. Keefe, R.W. Buchanan, R.M. Hamer, J.D. Kilts, D.W. Bradford, et al. 2009. Proof-of-concept trial with the neurosteroid pregnenolone targeting cognitive and negative symptoms in schizophrenia. Neuropsycopharmacology ; 34: 1885-903
Pearlstein T. 2010Premenstrual dysphoric disorder: out of the appendix. . ; 13 21 3
Amin Z. Mason G. F. Cavus I. Krystal J. H. Rothman D. L. Epperson C. N. 2007The interaction of neuroactive steroids and GABA in the development of neuropsychiatric disorders in women. ; 21 414 20
Bäckström T. Andreen L. Birzniece V. Björn I. et al. 2003.The role of hormones and hormonal treatments in premenstrual syndrome. CNS Drugs. ; 17: 325-42.
Bloch M. Schmidt P. J. Danaceau M. Murphy J. Nieman L. Rubinow D. R. 2000Effects of gonadal steroids in women with a history of postpartum depression. ; 157 924 30
Uzunova V. Sheline Y. Davis J. M. Rasmusson A. Uzunov D. P. Costa E. Guidotti A. 1998Increase in the cerebrospinal fluid content of neurosteroids in patients with unipolar major depression who are receiving fluoxetine or fluvoxamine. ; 95 3239 44
Romeo E. Ströhle A. Spalletta G. di Michele F. Hermann B. Holsboer F. Pasini A. Rupprecht R. 1998Effects of antidepressant treatment on neuroactive steroids in major depression. ; 155 910 3
Rupprecht R. Rammes G. Eser D. Baghai T.C. et al. 2009.Translocator protein (18 kDa) as target for anxiolytics without benzodiazepine-like side effects. Science. ; 325: 490-3.
Rupprecht R. Papadopoulos V. Rammes G. Baghai T. C. Fan J. Akula N. Groyer G. Adams D. Schumacher M. 2010Translocator protein (18 kDa) (TSPO) as a therapeutic target for neurological and psychiatric disorders. . ; 9 971 88
Schüle C. Eser D. Baghai T. C. Nothdurfter C. Kessler J. S. Rupprecht R. 2011Neuroactive steroids in affective disorders: target for novel antidepressant or anxiolytic drugs? Mar 23. [Epub ahead of print]
Costa B. Da E. Pozzo B. Chelli N. Simola M. Morelli M. Luisi M. Maccheroni S. Taliani F. Simorini F. Da Settimo. C. Martini 2011Anxiolytic properties of a 2phenylindolglyoxylamide TSPO ligand: Stimulation of in vitro neurosteroid production affecting GABAA receptor activity. ; 36; 463-472.
Agis-Balboa R. C. Guidotti A. Whitfield H. Pinna G. 2010Allopregnanolone biosynthesis is downregulated in the prefrontal cortex/ Brodmann’s area 9 (BA9) of depressed patients. Neuroscience Meeting Planner. San Diego, CA: ,
van Broekhoven F. Verkes R. J. 2003Neurosteroids in depression: a review. ; 165 97 110
Uzunov D. P. Cooper T. B. Costa E. Guidotti A. 1996Fluoxetine elicited changes in brain neurosteroid content measured by negative ion mass fragmentography. ; 93 12599 12604
Pinna G. Dong E. Matsumoto K. Costa E. Guidotti A. 2003.In socially isolated mice, the reversal of brain allopregnanolone down-regulation mediates the anti-aggressive action of fluoxetine. Proc Natl Acad Sci USA ; 100 2035 2040
Pinna G. Costa E. Guidotti A. 2004Fluoxetine and norfluoxetine stereospecifically facilitate pentobarbital sedation by increasing neurosteroids. ; 101 6222 6225
Pinna G. Costa E. Guidotti A. 2009SSRIs act as selective brain steroidogenic stimulants (SBSSs) at low doses that are inactive on 5-HT reuptake. ; 9 24 30
Pinna G. 2010In a mouse model relevant for post-traumatic stress disorder, selective brain steroidogenic stimulants (SBSS) improve behavioral deficits by normalizing allopregnanolone biosynthesis. . ; 21 438 50
Nin M. Schuler L. A. Martinez R. Thomas M. Nelson G. Pinna 2011Allopregnanolone and S-norfluoxetine decrease anxiety-like behavior in a mouse model of anxiety/depression. ; 83 215 216
Baulieu E. E. 1981Steroid hormones in the brain: several mechanisms. In: Steroid hormone regulation of the brain. Fuxe K, Gustafson JA, Wettenberg L (editors). ; Elmsford, NY: Pergamon; 3 14
Baulieu E. E. Robel P. Schumacher M. 2001Neurosteroids: beginning of the story. ; 46 1 32
Cheney D. L. Uzunov D. Costa E. Guidotti A. 1995Gas chromatographic-mass fragmentographic quantitation of 3α-hydroxy-5α-pregnan-20-one (allopregnanolone) and its precursors in blood and brain of adrenalectomized and castrated rats. ; 15 4641 4650
Guidotti A. Dong E. Matsumoto K. Pinna G. Rasmusson A. M. Costa E. 2001The socially-isolated mouse: a model to study the putative role of allopregnanolone and 5α-dihydroprogesterone in psychiatric disorders. ; 37 110 115
Stoffel-Wagner B. 2001Neurosteroid metabolism in the human brain. ; 145 669 679
Puia G. Santi M. R. Vicini S. Pritchett D. B. Purdy R. H. Paul S. M. Seeburg P. H. Costa E. 1990Neurosteroids act on recombinant human GABAA receptors. ; 4 759 765
Puia G. Vicini S. Seeburg P. H. Costa E. 1991Influence of recombinant gamma-aminobutyric acid-a receptor subunit composition on the action of allosteric modulators of gammaaminobutyricacid-gated Cl-currents. ; 39 691 696
Puia G. Mienville J. M. Matsumoto K. Takahata H. Watanabe H. Costa E. Guidotti A. 2003On the putative physiological role of allopregnanolone on GABAA receptor function. 44 49 55
Pinna G. Uzunova V. Matsumoto K. Puia G. Mienville J. M. Costa E. Guidotti A. 2000Brain allopregnanolone regulates the potency of the GABAA receptor agonist muscimol. ; 39 440 448
Lambert J. J. Belelli D. Peden D. R. Vardy A. W. Peters J. A. 2003Neurosteroid modulation of GABAA receptors. ; 71 67 80
Lambert J. J. Cooper M. A. Simmons R. D. Weir C. J. Belelli D. 2009Neurosteroids: endogenous allosteric modulators of GABAA receptors. ; 34 Suppl 1: S 48 58
Belelli D. Lambert J. J. 2005Neurosteroids: endogenous regulators of the GABAA receptor. ; 6 565 575
Majewska M. D. 1992Neurosteroids: endogenous bimodal modulators of the GABAA receptor. Mechanism of action and physiological significance. ; 38 379 395
D’Aquila P. S. Canu S. Sardella M. Spanu C. Serra G. Franconi F. 2010Dopamine is involved in the antidepressant-like effect of allopregnanolone in the forced swimming test in female rats. ; 21 21 8
Nin M. S. Salles F. B. Azeredo L. A. Frazon A. P. Gomez R. Barros H. M. 2008Antidepressant effect and changes of GABAA receptor gamma2 subunit mRNA after hippocampal administration of allopregnanolone in rats. ; 22 477 85
Rodríguez-Landa J. F. Contreras C. M. García-Ríos R. I. 2009Allopregnanolone microinjected into the lateral septum or dorsal hippocampus reduces immobility in the forced swim test: participation of the GABAA receptor. ; 20 614 622
Kita and Furukawa. 2008Involvement of neurosteroids in the anxiolytic-like effects of AC-5216 in mice. ; 89 171 8
Lonsdale and Burnham. 2007The anticonvulsant effects of allopregnanolone against amygdala-kindled seizures in female rats. ; 411 147 51
Mares P. Mikulecká A. Haugvicová R. Kasal A. 2006Anticonvulsant action of allopregnanolone in immature rats. ; 70 110 7
Martin-Garcia E. Pallares M. 2005The intrahippocampal administration of the neurosteroid allopregnanolone blocks the audiogenic seizures induced by nicotine. ; 1062 144 50
Jain N. S. Hirani K. Chopde C. T. 2005Reversal of caffeine-induced anxiety by neurosteroid 3-alpha-hydroxy-5-alpha-pregnane-20-one in rats. ; 48 627 38
Puia G. Vicini S. Seeburg P. H. Costa E. 1992Different sites of action of neurosteroids and benzodiazepines on natural and recombinant GABAA receptors. . ; 47 103 10
Puia G. Ducic I. Vicini S. Costa E. 1993Does neurosteroid modulatory efficacy depend on GABAA receptor subunit composition? . ; 1 135 42
Matsumoto K. Nomura H. Murakami Y. Taki K. Takahata H. Watanabe H. 2003Long-term social isolation enhances picrotoxin seizure susceptibility in mice: up-regulatory role of endogenous brain allopregnanolone in GABAergic systems. 75 831 835
Hosie A. M. Wilkins M. E. da H. M. Silva T. G. Smart 2006Endogenous neurosteroids regulate GABAA receptors through two discrete transmembrane sites. ; 444 486 9
Belelli D. Casula A. Ling A. Lambert J. J. 2002The influence of subunit composition on the interaction of neurosteroids with GABAA receptors. ; 43 651 61
Vicini S. 1991Pharmacologic significance of the structural heterogeneity of the GABAA receptor-chloride ion channel complex. ; 4 9 15
Agis-Balboa R. C. Pinna G. Zhubi A. Maloku E. Veldic M. Costa E. Guidotti A. 2006Characterization of brain neurons that express enzymes mediating neurosteroid biosynthesis. 103 14602 14607
Akk G. Shu H. J. Wang C. Steinbach J. H. Zorumski C. F. Covey D. F. Mennerick S. 2005Neurosteroid access to the GABAA receptor. ; 25 11605 11613
Pinault D. 2004The thalamic reticular nucleus: structure, function and ; 46 1 31
Serra M. Pisu M. G. Littera M. Papi G. Sanna E. Tuveri F. Usala L. Purdy R. H. Biggio G. 2000Social isolation-induced decreases in both the abundance of neuroactive steroids and GABA(A) receptor function in rat brain. . ; 75 732 40
Bortolato M. Devoto P. Roncada P. Frau R. Flore G. Saba P. Pistritto G. Soggiu A. Pisanu S. Zappala A. Ristaldi M. S. Tattoli M. Cuomo V. Marrosu F. Barbaccia M. L. 2011Isolation rearing-induced reduction of brain 5α-reductase expression: Relevance to dopaminergic impairments. 60(7-8):1301-8
Matsumoto K. Puia G. Dong E. Pinna G. 2007.GABAA receptor neurotransmission dysfunction in a mouse model of social isolation-induced stress: possible insights into a non-serotonergic mechanism of action of SSRIs in mood and anxiety disorders. Stress ; 10: 3-12.
Dong E. Matsumoto K. Uzunova V. Sugaya I. Costa E. Guidotti A. 2001Brain 5α-dihydroprogesterone and allopregnanolone synthesis in a mouse model of protracted social isolation. USA ; 98 2849 2854
Pibiri F. Nelson M. Guidotti A. Costa E. Pinna G. 2008Decreased allopregnanolone content during social isolation enhances contextual fear: a model relevant for posttraumatic stress disorder. ; 105 5567 5572
Agis-Balboa R. C. Pinna G. Kadriu B. Costa E. Guidotti A. 2007Downregulation of 5α-reductase type I mRNA expression in cortico-limbic glutamatergic circuits of mice socially isolated for four weeks. ; 104 18736 41
Matsumoto K. Uzunova V. Pinna G. Taki K. Uzunov D. P. Watanabe H. Mienvielle J. M. Guidotti A. Costa E. 1999Permissive role of brain allopregnanolone content in the regulation of pentobarbital-induced righting reflex loss. ; 38 955 963
Nelson M. Pinna G. 2011S-norfluoxetine infused into the basolateral amygdala increases allopregnanolone levels and reduces aggression in socially isolated mice. ; 60 1154 1159
Rodgers R. J. Johnson N. J. 1998Behaviorally selective effects of neuroactive steroids on plus-maze anxiety in mice. ; 59 221 32
Frye C. A. Rhode M. E. 2006Infusions of 5alpha-pregnan-3alpha-ol-20-one (3alpha,5alpha-THP) to the ventral tegmental area, but not the substantia nigra, enhance exploratory, anti-anxiety, social and sexual behaviours and concomitantly increase 3alpha,5alpha-THP concentrations in the hippocampus, diencephalon and cortex of ovariectomised oestrogen-primed rats. ; 18 960 75
Bitran D. Hilvers R. J. Kellogg C. K. 1991Anxiolytic effects of 3 alpha-hydroxy-5 alpha[beta]-pregnan-20-one: endogenous metabolites of progesterone that are active at the GABAA receptor. ; 561 157 61
Wieland S. Lan N. C. Mirasedeghi S. Gee K. W. 1991Anxiolytic activity of the progesterone metabolite 5 alpha-pregnan-3 alpha-o1-20-one. ; 565 263 8
Deo G. S. Dandekar M. P. Upadhya M. A. Kokare D. M. Subhedar N. K. 2010Neuropeptide Y Y1 receptors in the central nucleus of amygdala mediate the anxiolytic-like effect of allopregnanolone in mice: Behavioral and immunocytochemical evidences. ; 1318 77 86
Engin E. Treit D. 2007The anxiolytic-like effects of allopregnanolone vary as a function of intracerebral microinfusion site: the amygdala, medial prefrontal cortex, or hippocampus. ; 18 461 70
Frye C. A. Paris J. J. Rhodes M. E. 2009Increasing 3alpha,5alpha-THP following inhibition of neurosteroid biosynthesis in the ventral tegmental area reinstates anti-anxiety, social, and sexual behavior of naturally receptive rats. ; 137 119 28
Akbarian S. Huntsman M. M. Kim J. J. Tafazzoli A. Potkin S. G. Bunney W. E. J. Jones E. G. 1995GABAA receptor subunit gene expression in human prefrontal cortex: comparison of schizophrenics and controls. 5 550 60
Dean B. Hussain T. Hayes W. Scarr E. Kitsoulis S. Hill C. Opeskin K. Copolov D. L. 1999Changes in serotonin2A and GABA(A) receptors in schizophrenia: studies on the human dorsolateral prefrontal cortex. ; 72 1593 9
Lewis D. A. 2000GABAergic local circuit neurons and prefrontal cortical dysfunction in schizophrenia. . ; 31 270 6
Ishikawa M. Mizukami K. Iwakiri M. Hidaka S. Asada T. 2004. GABAA receptor gamma subunits in the prefrontal cortex of patients with schizophrenia and bipolar disorder. Neuroreport ; 15 1809 12
Rudolph U. Möhler H. 2004Analysis of GABAA receptor function and dissection of the pharmacology of benzodiazepines and general anesthetics through mouse genetics. . ; 44 475 98
Rudolph U. Crestani F. Benke D. Brünig I. Benson J. A. Fritschy J. M. Martin J. R. Bluethmann H. Möhler H. 1999Benzodiazepine actions mediated by specific gamma-aminobutyric acid(A) receptor subtypes. ; 401 796 800
Pinna G. Agis-Balboa R. C. Zhubi A. Matsumoto K. Grayson D. R. Costa E. Guidotti A. 2006Imidazenil and diazepam increase locomotor activity in mice exposed to protracted social isolation. ; 103 4275 4280
Crestani F. Martin J.R. Möhler H. Rudolph U. 2000. Mechanism of action of the hypnotic zolpidem in vivo. Br J Pharmacol. ; 131: 1251-4.
Smith S. S. Gong Q. H. Hsu F. C. Markowitz R. S. ffrench-Mullen J. M. Li X. 1998GABA(A) receptor alpha4 subunit suppression prevents withdrawal properties of an endogenous steroid. . ; 392 6679 926 30
Valzelli L. 1973The "isolation syndrome" in mice. . ; 31 305 20
Valzelli L. 1981Psychopharmacology of aggression: an overview. ; 16 39 48
Matsumoto K. Cai B. Satoh T. Ohta H. Watanabe H. 1991Desipramine enhances isolation-induced aggressive behavior in mice. ; 39 167 70
Sanacora G. Saricicek A. 2007GABAergic contributions to the pathophysiology of depression and the mechanism of antidepressant action. 6 127 140
Sanacora G. 2010Cortical Inhibition, gamma-aminobutyric acid, and major depression: there is plenty of smoke but is there fire? . ; 67 397 8
Bremner J. D. Innis R. B. Southwick S. M. Staib L. Zoghbi S. Charney D. S. 2000Decreased benzodiazepine receptor binding in prefrontal cortex in combat-related posttraumatic stress disorder. ; 157 1120 6
Fujita M. Southwick S. M. Denucci C. C. Zoghbi S. S. Dillon M. S. Baldwin R. M. Bozkurt A. Kugaya A. Verhoeff N. P. Seibyl J. P. 2004Innis RB: Central type benzodiazepine receptors in Gulf War veterans with posttraumatic stress disorder. ; 56 95 100
Vaiva G. Thomas P. Ducrocq F. Fontaine M. Boss V. Devos P. Rascle C. Cottencin O. Brunet A. Laffargue P. Goudemand M. 2004Low posttrauma GABA plasma levels as a predictive factor in the development of acute posttraumatic stress disorder. ; 55:250-4,
Brady K. Pearlstein T. Asnis G.M. Baker D. Rothbaum B. Sikes C. Farfel G.M. 2000. Efficacy and safety of sertraline treatment of posttraumatic stress disorder. JAMA ; 283 1837 1844
Davidson J. R. T. Rothbaum B. O. van der Kolk B. A. Sikes C. R. Farfel G. M. 2001Multicenter, double-blind comparison of sertraline and placebo in the treatment of posttraumatic stress disorder ; 58 485 492
104. R.D. Marshall, K.L. Beebe, M. Oldham, R. Zaninelli. 2001. Efficacy and safety of paroxetinetreatment for PTSD: A fixed-dose, placebo-controlled study. Am J Psychiatry ; 158:1982-1988
Tucker P. Zaninelli R. Yehuda R. Ruggiero L. Dillingham K. Pitts C. D. 2001Paroxetine in the treatment of chronic posttraumatic stress disorder: Results of a placebo-controlled, flexible-dosage study. ; 62 860 868
Friedman M. J. Marmar C. R. Baker D. G. Sikes C. R. Farfel G. M. 2007Randomized, double-blind comparison of sertraline and placebo for posttraumatic stress disorder in a Department of Veterans Affairs setting. ; 68 711 720
Gulinello M. Gong Q. H. Smith S. S. 2003Progesterone withdrawal increases the anxiolytic actions of gaboxadol: role of alpha4betadelta GABA(A) receptors. ; 14 43 46
Kaminski R. M. Livingood M. R. Rogawski M. A. 2004Allopregnanolone analogs that positively modulate GABA receptors protect against partial seizures induced by 6-Hz electrical stimulation in mice. ; 45 864 7
Marx C. E. Van Doren M. J. Duncan G. E. Lieberman J. A. Morrow A. L. 2003Olanzapine and clozapine increase the GABAergic neuroactive steroid allopregnanolone in rodents. ; 28 1 13
Genazzani A.D. Stomati M. Bernardi F. Pieri M. Rovati L. Genazzani A.R. 2003. Long-term low-dose dehydroepiandrosterone oral supplementation in early and late postmenopausal women modulates endocrine parameters and synthesis of neuroactive steroids. Fertility & Sterility ; 80: 1495-501.
Schmidt P.J. Daly R.C. Bloch M. Smith M.J. Danaceau M.A. Simpson L.S.C. Murphy J.H. Haq N. Rubinow D.R. 2005. Dehydroepiandrosterone monotherapy in midlife-onset major and minor depression. Arch Gen Psychiatry ; 62: 154-162.
Griffin L. D. Mellon S. H. 1999Selective serotonin reuptake inhibitors directly alter activity of neurosteroidogenic enzymes. ; 96 13512 7
Trauger J. W. Jiang A. Stearns B. A. Lo . P. V. Grasso 2002Kinetics of allopregnanolone formation catalyzed by human 3 alpha hydroxysteroid dehydrogenase Type III (AKR1C2). ; 41 13451 13459
Papadopoulos V. Baraldi M. Guilarte T. R. Knudsen T. B. Lacapère J. J. Lindemann P. Norenberg M. D. Nutt D. Weizman A. Zhang M. R. Gavish M. 2006Translocator protein (18kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function. ; 27 402 9
Compagnone N. A. Mellon S. H. 2000Neurosteroids: biosynthesis and function of these novel neuromodulators. ; 21 1 56
Reddy D. S. Kulkarni S. K. 1996Role of GABA-A and mitochondrial diazepam binding inhibitor receptors in the anti-stress activity of neurosteroids in mice. ; 128 280 92
Romeo E. Auta J. Kozikowski A. P. Ma Papadopoulos D. V. Puia G. Costa E. Guidotti A. 1992Aryl-3-indoleacetamides (FGIN-1): a new class of potent and specific ligands for the mitochondrial DBI receptor (MDR). ; 262 971 8
Korneyev A. Pan B. S. Polo A. Romeo E. Guidotti A. Costa E. 1993Stimulation of brain pregnenolone synthesis by mitochondrial diazepam binding inhibitor receptor ligands in vivo. ; 61 1515 24
Kita A. Kohayakawa H. Kinoshita T. Ochi Y. Nakamichi K. Kurumiya S. Furukawa K. Oka M. 2004Antianxiety and antidepressant-like effects of AC-5216, a novel mitochondrial benzodiazepine receptor ligand. ; 142 1059 72
Maguire J. Mody I. 2009Steroid hormone fluctuations and GABAA R plasticity. ; 34: Suppl 1: S 84 90