Effect of different formulations of mixed blue green algae on the growth of Paddy plants under greenhouse condition.
\r\n\tThe human microbiota consists of a wide variety of bacteria, viruses, fungi, and other single-celled animals that live in the body while microbiome is the name given to all of the genes inside these microbial cells. Recently, there has been renewed interest in the role played by microbiota and microbiome in both human health and human disease. A correct equilibrium between the human host and their microorganisms is important for an appropriate physiological function.
\r\n\tMicroorganisms have evolved alongside humans and form an integral part of life, carrying out a range of vital functions. They are implicated in both health and disease, and research has found links between bacterial populations, whether normal or disturbed, and the following diseases: asthma, cancer, diabetes, obesity, heart disease and, neurological and neurodegenerative diseases.
\r\n\tThe chapters of this book aim to present outstanding research on biochemical, genetics, clinical, molecular and behavioral fields about microbiota-gut-brain axis with emphasis in how neuropeptides such as brain derived factor (BDNF), substance P, calcitonin gene-related peptide and neuropeptide Y (NPY), vasoactive intestinal polypeptide, somatostatin and corticotropin-releasing factor are also likely to play a role in the bidirectional gut-brain communication. In this capacity they may influence the activity of the gastrointestinal microbiota and its interaction with the gut-brain axis.
\r\n\tIt will be shown evidence that neuropeptides represents a challenge in understanding the complex interactions between gut and brain. Although their precise role in the microbiota-gut-brain axis has not yet been defined, neuropeptides play an important role in this respect. For instance, a growing field of work is implicating the microbiota-microbiome in a variety of psychological processes and neuropsychiatric disorders. These include mood and anxiety disorders, neurodevelopmental disorders such as autism spectrum disorder and schizophrenia, and even neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. These brain disturbances have been linked to molecular and biochemical alterations in the course of neurodevelopment so, the research in this area has established different approaches (nutritional, immunological, energy homeostasis), to find the role played by the gut microbiota-microbiome in the etiology of the aforementioned brain disorders.
With the development of technologies such as industrial robots and computer image processing, a series of research and experiments on intelligent picking robots have been carried out in Japan and other related countries, such as tomato, apple, and grape picking robots [1]. Research on picking robots has focused on two parts: the first is a hardware device that can achieve rapid picking, that is, how to design a stable, efficient, and adaptable mechanical and visual sensing system, and the second is to design intelligent software for continuous operations, that is, to accurately identify, distinguish, and locate targets and to solve the problem of task planning and task scheduling during continuous picking operations.
\nIn the research field of picking robots, Kondo et al. [2] designed a tomato picking robot using a 7-degree-of-freedom manipulator. It used fingers and pneumatic nozzles in conjunction with a color camera to complete the picking operation. The experiment achieved a picking success rate of about 70% [3]. Tanigaki et al. [4] developed a cherry picking robot using a four-degree-of-freedom manipulator and a specially designed end effector with suction and shear functions. The visual part uses a light emitter, a photodetector, and a scanning device. The fruit was picked in 14 s, and the success rate was about 84%. The CROPS plan completed in 2014 was jointly completed by many European countries and units and aims to develop a modular picking robot system for different mission scenarios. The greenhouse bell pepper picking robot platform completed in the experiment in the Netherlands [5] used a 9-degree-of-freedom manipulator, two color CCD cameras and a depth-measuring camera, and its end effector was also equipped with a small camera to complete the picking with higher accuracy. Taqi et al. [6] have developed small household cherry tomato picking robots that can achieve very accurate picking tasks in specific environments.
\nThe continuous operation of the picking robot can make intelligent decisions on multi-task under multi-objective scenarios and plan the operation according to the picking needs. The research goal is that the picking robot system can intelligently select and pick fruits that meet the picking conditions, thereby greatly improving the degree of automation of the picking process and improving the quality of the harvested fruits. Because the picking robot is still in the laboratory research stage, the research on continuous operation is also very limited, and it is basically in its infancy. Japan’s Nagata et al. [7] used the shape to judge and classify strawberry quality. The accuracy in the experiment is acceptable, but the speed is much slower than artificial. Zhao et al. [8] investigated the visual recognition of apple maturity, using multi-spectral laser beams to complete fruit identification and positioning and ripeness judgment. Guo [9] and others judged strawberry maturity based on HIS color space algorithm. In the field test, the accuracy of the goal of picking fruits with maturity of 80% or more was more than 90%. Wang [10] and Ling et al. [11] carried out research on selective harvest information acquisition and path planning of tomato picking robots. Multi-sensor information fusion method was used for tomato quality detection and classification and selective picking decision. The appearance maturity of the fruit is detected by the H-means in the computer image, the fruits are classified in real time according to the agricultural industry standards, and the selective harvesting decision is made through the progressive identification of feature information and the fusion decision. At harvest time, path planning is performed on multiple targets through an optimization algorithm and then boxed by level.
\nThis chapter mainly focuses on developing an intelligent software system for the continuous operation of the dual-arm picking robot in a plant factory. Typically, the semi-structure environment of the greenhouse poses challenges for autonomous operations of the robot, and the complex tasks mainly include identification and positioning under variable light conditions, selective picking in multi-cluster growth environment, and complex multi-task programming. For the difficulty of developing the software system, a hierarchical modular software system framework is designed. Moreover, a scheduling method of functional modules is designed based on the idea of finite state machine for the complex multi-task planning problem of tomato continuous picking process. The task scheduling design based on the Finite State Machine (FSM) reduces the difficulty of development work and improves the efficiency of development.
\nThis chapter is organized as follows: Section 2 briefly describes the hardware structure of a dual-arm robot, and Section 3 presents the software framework with the highlights of deploying SMACH- and ViSP-based nodes in a ROS development environment. The experimental results are also included at the end of Section 3.
\nThe semi-structured operating environment in the plant factory is relatively complicated, such as the occlusion of fruit branches and leaves, the challenging grasping shape of the fruit, the changing light, and the variety of tasks, which requires to consider the multi-tasking ability of the robot when designing the hardware of the picking robot. At the same time, the complexity of the picking environment requires that the execution of the robot be robust to the environment in which the target is located, only in this way can it pick fruits in different states. Based on the above two design goals, we designed a dual-arm picking robot to simulate human picking operations.
\nThe mechanical structure of the robot body mimics human arms, and the left and right arms each have three joints: a vertical lift joint, a boom rotation joint, and a forearm rotation joint. The vertical lifting joint is driven by a servo motor to drive the roller screw. The actual effective stroke is about 300 mm. The big and small arm joints are driven by a servo motor connected to a harmonic reducer. There is a waist joint between the body and the base, which can provide 360° rotary motion. The single-arm movement of the robot is similar to that of the SCARA robot. The vertical positioning is achieved by the lifting joint, and the rotation of the large and small arms realizes the positioning in the plane. There are three degrees of freedom in motion. Each of the left and right forearms is designed with a mounting flange surface, and end effectors can be installed as required. The dual-arm robot base is installed on a mobile cart and is transported along the track to different picking points for picking operations.
\nDue to the limitation of the freedom of the robot body, for complex tomato picking environments, it is not enough to rely only on the freedom of the arms, so we can use the design of the end effector to increase the freedom of our robot and enable the robot to flexibly complete the picking operation. We designed a shearing end effector on the cutting hand of the dual-arm robot and designed a suction-type end sleeve on the auxiliary hand to fix the target tomato and assist the hand in picking (Figure 1).
\nThe structure of the robot body.
As shown in Figure 2, the world coordinate system \n
Robot coordinate system.
We use the tf function package [12] provided by ROS to maintain the transformation relationship between coordinate systems. After the robot model is built according to urdf, the system will automatically broadcast the transformation relationships between all the coordinate systems. The picking robot coordinate system is shown in Figure 3.
\nThe picking robot tf coordinate system under Rivz.
Based on the above coordinate system, using the rosrun tf view_frames command, we can view the tf tree of the dual-arm picking robot, as shown in Figure 4.
\nThe tf tree of robot.
The communication architecture of the dual-arm picking robot is shown in Figure 5. The motor driver is a Gold series motor driver produced by Elmo and is equipped with the same series of multi-axis controllers. The manufacturer has provided a complete motor driver to multi-axis controller communication protocol and communication protocol implementation and does not require customers to conduct secondary development. The multi-axis controller uses Modbus TCP communication as the communication between the lower computer and the industrial computer. It is connected to it through an Ethernet cable.
\nPicking robot communication architecture.
In the process of programming, we generally manually divide the tasks of the robot. Once the tasks are effectively divided, we can stack the tasks with the smallest functional components, so the difficulty of task division is how to use human prior knowledge to divide the minimum granularity of robot skills, which is a learning process. At the same time, how to easily and effectively combine the divided components is also an important part of completing the task simulation.
\nThe system architecture describes the functional structure of the subdivision and the topological relationship between them and a series of specifications that need to be set for subsequent development. The basic requirements of software engineering include modularity, code reuse, and function sharing. Using a common framework is helpful for decomposing development tasks and code migration. Robot software also follows the general rules of software engineering. Architecture is how you break up the robot’s functions and organize your code. A clear architecture that matches the project directly determines your development efficiency and even the success or failure of the final function. There are two main approaches to robot system architecture: SPA architecture and behavior-based architecture.
\nThe software system architecture is “Sense Plan Act”(SPA), as shown in Figure 6. The robot maps the external environment space through sensors and uses a certain modeling method to structure and model the perception information and then analyzes the model to plan the robot’s actions. Finally, the action instructions are executed in the environment to achieve a complete interactive process.
\nSPA work pattern.
The typical software architecture in a SPA robot system is a three-layer architecture: the perception layer, the planning layer, and the motion control layer, as shown in Figure 7. The perception layer receives and processes the sensor data, the planning layer plans the motion trajectory, and the motion control layer ensures the accurate execution of the movement. The SPA robot software system architecture pays more attention to the perception and modeling of the world because this is the basis for the accuracy of subsequent planning and movement.
\nSPA layered design.
The behavior-based software system architecture is a top-down software design. The small functions of each robot are packaged into individual small modules. All functional modules can be executed in parallel without prioritization. A robot task can be understood as an organic composition of functional modules (Figure 8).
\nAction-based software system architecture.
To a certain extent, all robot actions are responses to stimuli (inputs). This stress mode avoids the thinking logic in the SPA architecture and facilitates the rapid action response. In order to achieve the task, we can design a control scheme to change the stress level of the action. Therefore, we need a global controller to coordinate the choice of actions in order to achieve our intended purpose. The behavior-based software design framework has good flexibility, but it increases the difficulty of control. When multiple actions can affect the output, problems are easy to occur.
\nTherefore, combined with the SPA software architecture and behavior-based software architecture, we design a software framework that combines the advantages of both architectures for continuous operation of a dual-arm picking robot. Its characteristics are as follows:
Hierarchical modular design: The software architecture absorbs the advantages of the SPA architecture and also adopts a hierarchical design. The layered design is mainly logical, which makes it easier for users to understand the working mode of the robot. At the same time, it also absorbs the advantages of behavior-based architecture, that is, functional modularity. Based on the analysis and understanding of the robot’s internal architecture, we divide the functions of the robot into seven modules, each of which is functionally independent of each other. Combining the above two is a hierarchical modular design, which divides the functional modules into a specific layer according to the attributes of the functions, thus strengthening the logic of the system.
Finite state machine control: In order to solve the shortcomings of behavior-based architecture, we have designed a task planning module based on finite state machines, which is used to schedule and control the execution order of each functional module to complete a specific task.
Layering is an important concept in software design. The division of layers provides a framework for business decomposition and simplifies many thinking processes. Considering the design characteristics of the software system and the functional features of the robot, the entire software architecture can be divided into four layers: presentation layer, application layer, sense layer, and data layer. The hierarchical modular design architecture is shown in Figure 9.
Presentation layer: Presentation layer has more business logic requirements. We designed the presentation layer based on the QT architecture. The entire presentation layer includes several main components as shown in Figure 10: the RVIZ module displays the model of the robot and other visual information; the image module displays the video image information collected by the current robot; the node module monitors all current node information; and the DashBoard module provides users with a function module for manually operating the robot; shell module provides command line functions; console module displays all log information executed by the system; reconfigure module provides users with a convenient tool for changing model parameters; and diagnostic module provides real-time robot monitoring information.
Application layer: Application layer focuses on the task execution of a single robot. It is separated from the implementation of specific functions and uses a combination of function modules to coordinate a task.
Sense layer: Sense layer is responsible for the interaction between the software system and the hardware. There are both a visual module responsible for environmental perception and a motion module responsible for the motion control of the upper and lower computers. Sense layer is a description of the robot’s capabilities.
Data layer: Data layer serves the data generated by the system. Part of the data generated by the system is stored in a local database for real-time decision making of the system. One part is uploaded to the server through the network and is fused with data from other robots and other time dimensions to plan the continuous operation of the robot.
Layered modular architecture design.
Presentation layer.
The criteria for the division of functional modules are to reduce coupling, relatively independent functions, and high code repeatability. According to the robot’s task module, the software system can be divided into motion (motion_pkg), control (control_pkg), vision (vision_pkg), visualization (visualization_pkg), collaboration (coorperation_pkg), database (database_pkg), and network (network_pkg). Each function module is represented as a function package at the file system layer. There can be multiple nodes in a package, and different nodes can be written in different programming languages.
\nFinite state machine (FSM) is a mathematical model of computational science. The objects it represents can be in a limited number of states at any one time. When an external input occurs, the system responds to the external input, and the FSM can conditionally transition from one state to another. This process is called an action. In computer science, finite state machines are widely used for modeling application behavior, hardware circuit system design, software engineering, compilers, network protocols, and computation and language research. FSM can be defined by the present state, condition, action, and substate. The specific interpretation is as follows:
Present state: The current state.
Condition: The premise of triggering an action can also be considered as an event. When a condition is filled, an action will be triggered.
Action: The operation performed when the conditions are met and can be regarded as a unit of calculation or transaction processing. After the action is completed, it can be transferred to a new state, it can still be in the original state, or it can be terminated.
Substate: The state after the present state transition. When different actions occur and different conditions are generated, a state may transition to a different substate. Once the transition is completed, it becomes the present state.
As shown in Figure 11, a task can be represented by a state transition diagram.
\nFinite state machine.
SMACH [13, 14, 15], which refers to “State Machine,” is a powerful and scalable Python-based library for hierarchical state machines. The SMACH library does not depend on ROS and can be used in any Python project. The executive_smach stack, however, provides very nice integration with ROS, including smooth actionlib integration and a powerful SMACH viewer to visualize and introspect state machines. The SMACH core library is lightweight and mainly provides two interfaces: State and Container.
\nState: The state represents the state being executed. Each state has some potential outputs. The State class outputs the result by implementing the blocking function execute().
\nContainer: The container is a collection of one or more states that enforces some strategy. The simplest container is a State machine. A SMACH state machine can be viewed as a state flow graph, where each node is an execution state (the robot is performing a certain action), and the edges connecting the nodes represent transitions between states. The State machine itself can also be regarded as a state and has its own output, so they can be combined in layers to complete a complex task.
\n\nFigure 12 shows an example state machine [16].
\nSMACH state machine example.
SMACH uses action files to define communication protocols between different states. The structure of the action file is simple and clear, as shown in Figure 13. Three data definition areas are separated by three underscores. The first area defines the message format of the request, and the middle area defines the returned result (result message format, the bottom area defines the intermediate information feedback) message format. Each area can contain multiple data type, and the system will automatically compile the action file into three message files during the compilation process, so the message format for communication between states is actually the message format provided by ROS.
\nFile format of action.
SMACH provides a general state type to support invocation while providing a special state class SimpleActionState as a proxy for actionlib. During the construction of the SimpleActionState object, the corresponding actionlib client is started by default. The user can define a goal in the constructor and create a callback function to process the data returned by the actionlib server.
\nFor the picking robot to perform a continuous picking task, we can use the state transition process to describe it. The detailed description of the state transition is as follows: first enter the startup state, start the picking robot platform, wait for the initialization of each component, and perform a startup self-test. If any component fails to initialize and is in a fault state, the startup fails, and then the system is placed in the error state, stopping working and the task ends.
\nIf the startup is successful, the platform moves into the state, the mobile platform moves to the first picking point, and data collection state and the tomato scanning state are started at the same time. The data collection state collects tomato information in the current status and uploads it to the database of the server; the tomato scan state checks whether there are tomatoes suitable for picking in the viewing area. If not, restart the platform moving state and move the robot to the next picking point; if so, first analyze all the tomato position information and pass the spatial attitude information of the first target tomato to the kinematics solution state according to the predetermined rules, and then program performs kinematics calculation and motion planning. If the tomato is unreachable, the information of the next target tomato is passed to the kinematics solution state, and so on until the last tomato is reached. If it is determined that the tomato is unreachable, the platform is moved to the next picking point. If it is judged that the tomato is reachable, the calculated right arm motion information is transmitted to the robot motion state, and this state sends the trajectory information of the right arm motion to the lower computer and simultaneously detects the joint motion position information during the execution of the lower computer. After the right arm moves to the target position, it enters the suction state. The system starts the suction device to fix the tomatoes and move them to a suitable position, which is convenient for the left arm to cut hands. The kinematics solution state is started again, and the left arm motion information is solved and transmitted to the robot motion state to control the left arm to move near the target. The start of the visual servoing state is close to the target tomato precisely, and the cutting state is started after the arrival, the pneumatic shear transposition of the left arm is started, and the tomato is cut. After completing a tomato pick, pick the next goal planned. Repeat until the last picking point.
\nAccording to the task execution process and state transition process described above, the designed state transition diagram is shown in Figure 14.
\nContinuous picking status flow.
In SMACH, we use SimpleActionState to directly simulate the server side of actionlib and define a state machine with 10 states to control the robot to complete a comprehensive picking job.
DO_START: start state.
DO_MOVEBASE: mobile platform mobile status.
DO_TOMATO_SCAN: tomato scanning status.
DO_SPATIAL_TEMPORAL: data collection status.
DO_KINEMATICS: state of kinematics solution.
DO_MOVE_ROBOT: left and right arm movement status.
DO_MOVE_ENDEFFECTOR: end effector status.
DO_ERROR: fault status.
DO_STOP: emergency stop status.
DO_VISUALSERVOING: visual servoing status.
Based on the software framework design and operation requirements of the dual-arm picking robot, based on the functional division of each part, a vision module, an eye-hand coordination module, and a task planning module are mainly designed and installed in the industrial computer. Each module further refines the functions and can be divided into functional nodes. Nodes are the minimum functional modules in ROS, which regard as the ultimate goal of the design.
\nIn the dual-arm acquisition robot eye-hand system, the main component of the “eye” is the camera, including a binocular camera mounted on the robot’s head and a monocular camera mounted on the arm. Based on the ROS framework, we designed three nodes to complete the environment perception function: binocular camera image acquisition node (dual_eye_image_capture), monocular camera image acquisition node (single_eye_image_capture), and image processing node (image_processing). The actual recognition effect is shown in Figure 15. There are three valid tomatoes in the image. The system recognizes all tomatoes and marks the positions of the tomatoes in the picture that need to be picked first according to the rules.
\nTomato identification interface.
The binocular camera acquisition node uses the two original images collected by the left and right sensors of the Bumblebee2 camera to finally generate five images: left and right eye corrected color images, left and right eye corrected gray images, and 3D point clouds (Figure 16).
\nFive pictures generated by Bumblebee2 camera. 1. Gray image of left eye after correction. 2. Color image of left eye after correction. 3. Gray image of right eye after correction. 4. Color image of right eye after correction. 5. Point cloud image.
The collection process of the binocular camera is shown in Figure 17. The camera’s original data are read, and the data are packaged into a Bell template image; then, three color information is extracted from the Bell template image and assembled into the original color image. The eye image data are used to obtain corrected left and right eye color images and grayscale images. Next, the left and right eye images are used for stereo matching through the principle of triangulation to generate a 3D point cloud. In the end, all the five images generated were published, and the algorithm used in the image acquisition process was provided by the camera SDK.
\nBinocular image acquisition flow chart.
Based on the above process, we designed the binocular collection program UML as shown in Figure 18.
\nBinocular acquisition node UML design.
For monocular vision, we use a Daheng Mercury series industrial camera MER-500-7UC, which uses USB2.0 digital interface and provides free SDK and secondary development example source code under windows platform and Linux platform. We use the usb_camera package provided by ROS to collect monocular images, as shown in Figure 19.
\nImage captured by monocular camera.
The image processing node receives the collected planar image and point cloud image and provides different image processing function interfaces according to different business requirements. In the current task requirements, image processing nodes are required to complete the accurate two-dimensional recognition and accurate three-dimensional positioning of tomatoes. Therefore, the two-dimensional image dataset of the scene and the three-dimensional point cloud data are also required. Next, we introduce us from two directions. Image processing node design: first is the architecture design and functional flow of the image processing node as a functional interface, and the second is the specific implementation of related image processing algorithms.
\nThe entire software system is based on the C/S model architecture, using the actionlib function package provided by ROS, with the task planning node as the server, and requesting computing resources from the client of each functional unit. Image processing nodes are no exception. After receiving the image processing instructions and image data, the instructions are parsed to clarify the functional requirements, and then the required image data are extracted, input into the algorithm function for processing, and the results are finally returned to the server.
\nThe specific processing flow is shown in Figure 20. After initializing the node and actionlib server, start the service, wait for the goal sent by the client, and subscribe to the processing function. After receiving the instruction, analyze the source of the instruction. If the instruction originates from the spatial positioning of tomatoes, the processing steps are: first, use the tomato recognition algorithm based on image feature fusion to identify all tomatoes in the right eye image space of the binocular camera. If there are no tomatoes, return the results; if tomatoes are detected, plan the picking order. The rule is from bottom to top, left to right, and calculate the spatial position of the pick point, and finally return the result to the client. If the object recognition result triggers the harvesting task, the image collected by the monocular camera is used to extract the central image feature of the tomato.
\nImage processing node flow chart.
According to the above process, the design program UML is shown in Figure 21.
\nImage processing node UML design.
Our solution uses an eye-in-hand vision servo solution to achieve eye-hand coordination, as shown in Figure 22. The picking robot obtains the image information of the target fruit through a monocular camera installed on the picking hand, extracts the position information of the tomato features in the two-dimensional image, and makes a difference from the expected position information. The difference is used as the input of the visual servo control algorithm and then calculate the control output in real time, that is, the speed vector of the end effector, and then integrate this speed vector with time to calculate the next point that needs to reach the target position. Cycle back and forth to get a trajectory that gradually approaches the target position. The eye-hand correspondence is converted into the amount of motion of the joint, and the end of the robot arm moves accordingly to approach the target. The implementation process is shown in Figure 23.
\nVisual servo program of eye-in-hand.
Eye-hand coordination process.
ViSP [17] is an open source visual servo framework developed and maintained by the Lagadic team of the French National Institute of Information and Automation. It has the characteristics of hardware independence, scalability, and portability. In addition, ViSP also provides a complete library of basic functions, which can be combined with a variety of visual feature libraries; it also provides a simulation environment and interfaces with various hardware. Based on ViSP, we can complete functions such as visual tracking, fiducial marking, two-dimensional contour tracking, pose estimation, and so on. The goal of ViSP is to provide developers with a tool for rapid development of visual servo functions. The software framework of ViSP is shown in Figure 24. The entire framework is divided into three modules: one module provides vision models, vision servo control algorithms, and robot controller interfaces; the second module provides image processing algorithms, tracking algorithms and other machine vision algorithms; and the last module is a visualization module that provides a simulation and visual environment. All these features make ViSP very suitable for use as a core part of our module.
\nViSP software architecture.
The complete flowchart of eye-hand coordination is shown in Figure 25. After the node is initialized, the system initializes and starts the ‘/visual_servo’ actionlib service and subscribes to execute() to wait for the client to be awakened. After receiving the service request, start the visual servo loop. In the loop, program request the feature position of the tomato image from the vision module and make a difference from the expected position. If the difference exceeds the threshold Δs (Δs=2mm), the program will obtain the camera parameters, initialize the control model, and call the ViSP library function vpServo() to calculate the control output speed vector. Then, program integrates the velocity vector with time (t = 1s), motion module controls robot to move to the output position, and requests the tomato image feature position from the vision module again, then makes a difference with the desired position, and loops back and forth until the target image feature. The difference between the position and the desired image feature position is less than the threshold Δs, the visual servo loop is ended, and our execution result is returned.
\nEye-hand collaboration node flow chart.
\nFigure 26 shows the design of the eye-hand coordination node class. There are mainly two classes. The VisualServoCycleNode class is responsible for the loop and interaction with other modules. The VisualServoControlNode module is responsible for controlling the operation of the algorithm.
\nEye-hand coordination node class design UML diagram.
The task planning module mainly completes the design and implementation of a layered concurrent state machine for one pick, as shown in Figure 27:
\nTask planning node flow chart.
First we initialize the node, state machine, and user intermediate data, and then add the transformation relationship between the states of each state machine according to the state transition of the task design. Use the transition keyword to control the transition from the current state to the secondary state. At the same time, since each state is SimpleActionState, each state implements an actionlib client by default. You need to add an initialization function and a callback function callback() for each state. Start a state machine visualization service IntrospectionServer in the node, so that we can view the state transition diagram in SMACH_viewer and can monitor the state transition in real time. The data details of each state are shown in Figure 28.
\nFSM in SMACH_viewer.
\nFigure 29 shows that the running node diagram after all ROS nodes in the system is turned on. The node diagram is generated using the rqt_graph command. Each rectangular box represents a topic. The oval box represents a node, and the arrowed lines represent the subscription relationship between each other. Visualization of the node diagram makes the system architecture intuitive.
\nSystem function node diagram.
Since most of the eye-hand coordination and motion control are concurrent, the fluency of multitasks is verified under two plant factories and three greenhouses with different fruit status and illumination variations. The experimental results show that if total number of targets within the visual field is not more than three, the average picking time is less than 35 s.
\nThe contribution of this research mainly orients around the software engineering for manipulating the complex robot behavior. Although service robot leverages ROS for rapid development, classical tasks such as eye-hand coordination and continuous operation in an open scenario have not been systematically addressed. In this chapter, we advocate that if the complex robot behavior can be structured, then they can be modeled as Finite State Machines (FSM), and a “Sense Plan Act” (SPA) process can be implemented with a formal software architecture. Meanwhile, we demonstrate that ViSP and SMACH in ROS are beneficial frameworks for developing a dual-arm robot for autonomously harvesting the fruits in plant factory, which embodies the complexity of multi-task planning and scheduling in natural scenes. The experimental results show that the software engineering paradigm effectively improves the system reliability and scalability of the dual-arm harvesting robot.
\nThis work was supported by the National Natural Science Foundation of China (No. 51775333) and the Scientific Research Program of Shanghai Science and Technology Commission (No. 18391901000).
\nBlue green algae are present abundantly in rice fields and are important in helping to maintain rice fields fertility through nitrogen fixation. They are belongs to a group of ubiquitous photosynthetic prokaryotes which possessing the ability to synthesis Chlorophyll a and carryout an important role in nutrient recycling and the maintenance of organic matter in aquatic systems including lakes, rivers and wetland. Nitrogen fixing blue green algae are known to be a prominent component of the microbial population in wetland soils, especially rice fields, contributing significantly to the fertility as a natural bio-fertilizer.
Nitrogen fixation is one of the most important biological processes and, though, the atmosphere contains about 79% nitrogen, most of the plants cannot utilize it. They can utilize combined nitrogen, like ammonium, nitrate, nitrite; etc. This process is called biological nitrogen fixation.
Rice (Oryza sativa) is monocot plant, of the grass family (Poaceae). As a cereal grain, it is the most popular cereal worldwide, serving as a stable food for 39 countries and nearly half of the world’s population [1]. Globally rice is considered as dietary energy source providing 22% of total energy intake [2]. Rice is second highest worldwide produce and consumed stable food and increasing ratio of population demands more production of rice to meet its consumption [3].
Blue green algal species that thrived in rice field release small quantities of ammonia as the major fertilizing product, and small nitrogenous polypeptides during active growth, whereas most of the fixed products are made available mainly through autolysis and decomposition. They have an important role to play in crop production as promising biofertilizers. Here an attempt was made to study the different formulations of blue green algae from the paddy field with the following objectives: Isolation and mass culturing of blue green algae form the areas of selective southern districts of Tamil Nadu. The selective isolated blue green algae have been formulated with different adsorbent like alluvial soil, sand, charcoal, powdered paddy straw and analyzed the interaction effect of various for BGA on vegetative growth of paddy plant (Figure 1).
Effect of different formulations of mixed blue green algae on paddy plants under greenhouse condition.
The soil samples collected from the areas namely as Thiruvadanai, of Ramnad, Selugai and Amaravathipudur of Sivagangai and Sakkimangalam of Madurai (Figure 2). They were stored at room temperature and were used as samples for further research.
Sampling sites.
The BG 11 with nitrate and without nitrate medium was prepared and sterilized in autoclave for 121°C, 15 lb pressure for 20 min. After cooling, the samples were inoculated in the BG 11 medium for enrichment. The inoculated flasks were maintained at a temperature of 25°C and 12 h light and 12 h darkness (light intensity 3000 lux).
The blue green algal growth was observed and identifying the organisms under Labomed vision 2000 smart scope B6. The selective identified organisms were sub cultured in BG0 under lab and maintained for further analysis (Figure 3).
Sub culturing of isolated blue green algae.
The BGA mixture (10 ml of each Microcoleus, Microcystis, Phormidium and Gloecapsa) was added with 50 g of selective adsorbents (alluvial soil, sand, charcoal, powdered paddy straw). Then such combinations were shade dried under laboratory condition. After drying, such mixture was packed in polythene bags further study.
Seeds of Paddy variety CR-1009 were surface sterilized with hot water for 5 min and washed with sterile water repeatedly. Then these seeds were placed in hot water for 10 min to soften the seed coat. Sterile garden soil was used to fill the earthen pots 15 cm height; 52 cm diameter. About 5 kg of sterile soil were taken in each earthen pot which was mixed with different adsorbent formulated BGA. Seeds (15 Nos.) were sown in each pot and germinated seedlings were thinned out to 10 in each pot. The above experimental plants were maintained under greenhouse conditions. The sterilized tap water was used for irrigating the plants. Such experimental pots were assigned for the following treatments:
C—control (without organism)
T1—alluvial soil + mixed BGA
T2—sand + mixed BGA
T3—charcoal + mixed BGA
T4—powdered paddy straw + mixed BGA
The paddy plant vegetative growth (15th day) was measured with the following growth parameters.
The plant materials were cut into bits and weighed. Then they were dried in an oven at 90°C until the weight became constant.
The shoot and root lengths of the plants were measured using a meter-scale.
The number of leaves or leaflets was counted for each plant.
The experimental leaf tissue was estimated for chlorophyll by following the method of Arnon [4]. Fifty milligram of Leaf tissue was homogenized in 80% pre chilled acetone by using a mortar and pestle and centrifuged at 3000 rpm. The pellet was homogenized again with acetone and was centrifuged repeatedly till the pellet become pale. The collected supernatants were pooled and the absorbance of the supernatant was read at 645 and 663 nm.
The chlorophyll content (mg/g fr.wt) was calculated by using the following formula:
where l is the path of light length in cm (1 cm), V is the volume of the extract in ml and W is the fresh weight of the sample in g (Chlorophyll contents were expressed either as mg or μg for the plant samples).
The experimental fresh leaf tissue of the protein content was estimated by Lowry’s method [5]. About 50 mg of the leaf tissue was weighed and was homogenized in hot 80% ethanol and macerate in a mortar with pestle. The supernatant was discarded and the pellet was collected for the analysis purpose. The collected pellet was suspended in a suitable volume of 5% TCA in an ice-bath for 15 min. The pellet was re extracted once in hot absolute ethanol and twice with ethanol-ether mixture, every time discarding the supernatants after centrifugation. Such collected pellet contained proteins and nucleic acids.
The extracted protein sample was placed in 1 ml of sodium hydroxide at 100°C for 4–5 min. The alkaline copper reagent (5 ml) was added and allowed to stand at room temperature for 10 min. Then the folin phenol reagent (0.5 ml) was added rapidly and mixed immediately. After 30 min, the absorbance was measured at 750 nm in a UV–Visible Spectrophotometer. The quantity of protein in the sample was calculated with a standard curve prepared using bovine serum albumin of different concentrations.
The data collected in this study was subjected to statistical methods standard deviation bar charts and pie charts applied [6].
Blue green algae (cyanobacteria) play an important role in maintenance and build-up of soil fertility, consequently increasing rice growth and yield as a natural biofertilizer [7]. They are photosynthetic nitrogen fixers and are free living. Increase in water-holding capacity through their jelly structure [8].
Cyanobacteria are known to be one of the promising supplements to nitrogenous fertilizer, but the process biological nitrogen fixation, mediated through the enzyme nitrogenase may be inhibited in presence readily available nitrogen source. Supplementation of chemical fertilizer with blue green algae could conserve up to 30% of commercial fertilizer and it is generally believed that the nitrogen fixed by these organisms is made available to the rice plants through exudation or autolysis and microbial decomposition. Onkar et al. [9] in addition to contributing fixed nitrogen and adding organic matter to soil such blue green algae are also known to excrete growth promoting substances, solubilize insoluble phosphates, improve fertilizer use efficiency of crop plants and amend the physical and chemical properties of soils, increasing soil aggregate size, there by correcting soil compaction, reduce oxidizable matter of the soil and narrowing down the C:N ratio [10].
Nitrogen fixing filamentous cyanobacteria occurs in wide range of habitats mainly rice-field ecosystem and agricultural fields [11, 12]. In rice field among photosynthetic aquatic organisms, investigations have been emphasized more on isolation and identification of nitrogen fixing cyanobacterial populations in agro-ecosystems for sustainable agriculture.
Shelf-life of cyanobacteria biofertilizer can be augmented by selecting translucent packing material, dry mixing and paddy straw as a carrier [13]. Conventionally, soil has been used as a carrier for cyanobacterial biofertilizers whereas in one study it was reported that soil based inoculums have proved to be disadvantages due to poor inoculums loading, heavy contamination and its bulky nature [14, 15, 16]. Sugar cane waste; rice husk [17] and coconut coir [18] was developed as new carrier material [13]. Field trials conducted using straw based, soil based and multani mitti based BGA biofertilizer and it was reported that multani mitti based biofertilizer gave highest yield followed by straw based and soil based BGA inoculants [19].
In the present study the paddy field soil was collected from four different villages namely as Thiruvadanai of Ramnad, Selugai and Amaravathipudur of Sivagangai and Sakkimangalam of Madurai district and blue green algae were isolated as Microcoleus, Microcystis, Phormidium and Gloecapsa (Figure 4). These isolates were mixed and formulated in four different adsorbents—alluvial soil, sand, charcoal, and powdered paddy straw. The efficiency of such formulates blue green algae mixture on the morphological and physiological activity of paddy plant (15th day growth) was analyzed (Table 1). According to this all the formulated BGA (blue green algae) inoculated paddy plant showed progressive increase in shoot and root length, fresh and dry weight, number of leaves, chlorophyll and protein content when compared to control plant. Among these formulations the alluvial soil + BGA treated plants showed better growth by means of increase in chlorophyll and protein content which indicated that the photosynthetic and metabolic activity was enhanced due to this treatment. Blue green algae formulated with adsorbents influenced the paddy plant growth and also they contributed to improve the nitrogen fertility in soil.
Microscopic view of isolated blue green algae from soil samples of sampling paddy fields. (a) Microcoleus, (b) Microcystis, (c) Phormidium, and (d) Gloecapsa.
Growth parameters | Control | T1 | T2 | T3 | T4 |
---|---|---|---|---|---|
Shoot length (cm) | 13.17 ± 0.29 | 18.83 ± 0.29 | 15.5 ± 0.50 | 17.43 ± 0.40 | 18.13 ± 0.12 |
Root length (cm) | 2.13 ± 0.23 | 3.93 ± 0.12 | 3.37 ± 0.12 | 3.6 ± 0.10 | 3.87 ± 0.12 |
No of leaves | 2 | 3 | 2 | 3 | 3 |
Fresh weight (g) | 0.17 ± 0.00 | 0.21 ± 0.00 | 0.20 ± 0.00 | 0.207 ± 0.00 | 0.209 ± 0.001 |
Dry weight (g) | 0.043 ± 0.00 | 0.052 ± 0.00 | 0.0507 ± 0.00 | 0.043 ± 0.00 | 0.051 ± 0.00 |
Chlorophyll a (μg) | 0.0313 ± 0.001 | 0.485 ± 0.001 | 0.251 ± 0.002 | 0.388 ± 0.001 | 0.279 ± 0.001 |
Chlorophyll b (μg) | 0.0187 ± 0.001 | 0.1513 ± 0.001 | 0.074 ± 0.002 | 0.104 ± 0.001 | 0.080 ± 0.001 |
Effect of different formulations of mixed blue green algae on the growth of Paddy plants under greenhouse condition.
Values are mean of three replicates ± SD.
The shoot and root length and fresh and dry weight of the paddy plant treated with alluvial soil + Mixed BGA and powdered paddy straw+ Mixed BGA showed maximum (18.83 ± 0.29; 3.93 ± 0.12 cm and 0.21 ± 0.00; 0.052 ± 0.00 g & 18.13 ± 0.12; 3.87 ± 0.12 cm and 0.209 ± 0.001; 0.051 ± 0.00 g) growth when compared to control (13.17 ± 0.29; 2.13 ± 0.23 cm and 0.17 ± 0.00; 0.043 ± 0.00). The number of leaves in all treated plants including control was more or less same (2 or 3). But the chlorophyll a, b and total chlorophyll content was higher in (0.485 ± 0.001; 0.1513 ± 0.001; 0.1803 ± 0.001 μg) alluvial soil + Mixed BGA and charcoal + mixed BGA (0.388 ± 0.001; 0.104 ± 0.001; 0.140 ± 0.000 μg) compared to control plant (0.0313 ± 0.001; 0.0187 ± 0.001; 0.0377 ± 0.001 μg) (Table 1 and Figure 5). The other formulated BGA treated plants showed minimal chlorophyll contents. The protein content of treated paddy plant with alluvial soil (28%; 2.52 ± 0.02 mg) + Mixed BGA and charcoal + mixed BGA (29%; 2.52 ± 0.00 mg) was significantly maximum when compare the control (10%) paddy plant (0.873 ± 0.06 mg) (Figure 6).
Effect of different formulations of mixed blue green algae on the total chlorophyll (μg) content of Paddy plants under greenhouse condition. C, control (without organism); T1, alluvial soil + mixed BGA; T2, sand + mixed BGA; T3, charcoal + mixed BGA; T4, powdered paddy straw + mixed BGA.
Effect of different formulations of mixed blue green algae on the protein content (mg) of Paddy plants under greenhouse condition. C, control (without organism); T1, alluvial soil + mixed BGA; T2, sand + mixed BGA; T3, charcoal + mixed BGA; T4, powdered paddy straw + mixed BGA.
Katoh et al. [20] reported that Nostoc species are very useful in agricultural applications because of their nitrogen fixation activity, extracellular polysaccharide, photosynthetic system, and particularly desiccation tolerance ability and these properties help to improve the quality of nutrient poor soils. Wetland rice fields could provide an ideal condition for the growth of cyanobacteria, fixing 25–30 kg N ha−1 crop−1, and reducing the use of urea fertilizer in rice culture by 30% [21, 22]. Algalization of BGA in rice cultivation promotes organic forming without usage of chemical fertilizers and production of organic basmati rice has been reported to develop a potential export market in the country [23].
Cyanobacteria also improve soil characteristics by modifying texture size and subsequent aeration and enhancing carbon content and water holding capacity [24]. Such organisms are one of the major components of the nitrogen fixing biomass in paddy fields. The importance of cyanobacteria in agriculture for paddy cultivation is directly proportional to their ability to fix nitrogen and other positive effects for plants and soil. The nitrogen is the second limiting factor next to the water for plant growth in many fields and efficiency of this element is met by fertilizer [25].
Current study suggested that the efficiency of paddy plant growth was enhanced due to the application of formulated BGA with various adsorbents. Such blue green algae were generally applied as biofertilizers in agriculture for improving the soil fertility by the process of biological nitrogen fixation.
The blue green algae distributed in different environments. They are actively involved in the fixation of atmospheric nitrogen by the action of nitrogenase enzyme which is present in such organisms but not in plant cells. Microcoleus, Microcystis, Phormidium and Gloecapsa. were isolated from the paddy fields of Thiruvadanai, Selugai, Amaravathipudur, Sakkimangalam areas of Ramnad, Sivagangai and Madurai district. The isolated organisms were mass cultured under laboratory condition and mixed well. The BGA mixture formulated with alluvial soil, sand, charcoal and powdered paddy straw were treated on paddy plant showed significant growth compared to control plant. The present study concluded that the alluvial soil and powdered paddy straw formulated BGA promoted the plant growth by means of enhance the morphological growth but chlorophyll and protein content of the alluvial soil and charcoal formulated BGA treated plant showed was maximum. This indicated that the formulated BGA enhanced morphological and photosynthetic efficiency of the paddy plant under greenhouse condition. The application of such bio-mixture in agriculture for crop production not only increase crop yield which may maintain our environment eco-friendly.
The authors have expressed their sincere thanks to the President, Vice-President, Secretary, Principal, Head of the Department, Thiagarajar College, Madurai, Tamil Nadu, India for their encouragement, support and provided the necessary facilities for the successful completion of the research work. And also they expressed their sincere thanks to their family and friends for the successful supportive work.
Supporting women in scientific research and encouraging more women to pursue careers in STEM fields has been an issue on the global agenda for many years. But there is still much to be done. And IntechOpen wants to help.
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