DNA double-strand break (DSB) is considered most critical type of DNA damage. In eukaryote, DSB is repaired mainly through non-homologous end-joining (NHEJ) and homologous recombination (HR). Our understanding on the molecular mechanisms of these DNA repair mechanisms has been greatly deepened in the last two decades.
In NHEJ, DSB is first recognized by Ku protein (Fig.1 (1)), heterodimer consisting of Ku70 and Ku86 (also known as Ku80), which in turn recruits DNA-PK catalytic subunit (DNA-PKcs) (Fig.1 (2)). The comprex consisting of Ku70, Ku86 and DNA-PKcs is termed DNA-dependent protein kinase (DNA-PK). When the DSB are not readily ligatable, processing takes place prior to ligation (Fig.1 (3)). Processing might involve a number of enzymes depending on the shape of each DNA end and compatibility of two ends to be ligated: Artemis nuclease, DNA polymerase µ/λ, polynucleotide kinase/phosphatase (PNKP), Aprataxin (APTX) and Aprataxin and PNKP-like factor (APLF, also known as PALF, C2orf13 or Xip1). DSBs are finally joined by DNA ligase IV, which is in tight association with XRCC4 (Fig.1 (4)). XRCC4-like factor (XLF, also known as Cernunnos), is essential at this step, especially when two ends are not compatible.
In HR, a complex consisting of Mre11, Rad50 and Nbs1, termed MRN complex, is thought to play two important roles in the initial stage (Fig.1 (1’)): recruitment of ATM (Fig.1 (2’’)) and resection of one of the strands (Fig.1 (2’)). ATM is a protein kinase structurally similar to DNA-PKcs. Although ATM is thought to phosphorylate a great number of proteins as revealed by phosphoproteomic analyses, the phosphorylation of histone H2AX at Ser139 is thought one of the most important events, triggering signal transduction cascade involving mediator protein like MDC1 and ubiquitin ligases like RNF8 and RNF168. As Mre11 bears 5’-3’ exonuclease activity, MRN resects one of the DNA strands to generate single-stranded DNA (ssDNA), which serves as a probe for the search for homology. Replication protein A (RPA) binds to ssDNA (Fig.1 (2’)) and facilitate the formation of Rad51 filament in cooperation with BRCA2, PALB2, Rad52 and Rad51 paralogues (Fig.1 (3’)). RPA also recruites ATRIP, which in turn recruits ATR, another protein kinase structurally related to DNA-PK and ATM (Fig.1 (3’’)). ATR phosphorylate checkpoint kinase Chk1 to initiate signal transduction pathway leading to cell cycle checkpoints. Rad51 promotes strand exchange between homologous sequences (Fig.1 (4’)). Template-dependent strand synthesis is proceeded by replication machinery including PCNA and DNA polymerase δ and ε (Fig.1 (5’)). Finally, the junction of two DNA molecules (Holliday’s junction) are resolved by nucleases Mus81-Eme1, ERCC1-XPF or SLX1-SLX4 (Fig.1 (6’)). Alternatively, synthesized strand aneals with opposite end of DSB, detaches from the temprate strand, followed by synthesis and ligation of complementary strand (synthesis-dependent strand anealing; SDSA, not shown here).
Here, we will overview approaches to radiosensitization through the modification of DSB repair enzymes.
2. DNA-PK, ATM and ATR kinases as targets for radiosensitizer
DNA-PK was initially found in the extracts of HeLa cell, rabbit reticulocyte, Xenopus egg and sea urchin egg (Walker et al., 1985) and was purified from Hela cell nuclei as a 300-350 kDa protein, which is now called DNA-PKcs (Carter et al., 1990; Lees-Miller et al., 1990). Later it was found that Ku is an essential component of DNA-PK (Dvir et al., 1992, 1993; Gottlieb and Jackson, 1993). Furthermore, it was also shown that DNA-PK requires binding of DNA-PKcs to DNA ends via Ku to be activated, suggesting its possible role in sensoring DSBs (Gottlieb and Jackson, 1993). Ku86 was shown to be equivalent to XRCC5 (X-ray repair cross complementing) gene product, which is missing in X-ray sensitive rodent cell lines including
First reported selective inhibitor of DNA-PK is OK-1035, 3-cyano-5-(4-pyridyl)-6-hydrazonomethyl- 2-pyridone (Fig.2 A), which was found by screening of more than 10,000 microbial extracts and synthetic compounds (Take et al., 1995). IC50 (50% inhibitory concentration) on DNA-PK was 8 µM, which was more than 50-fold lower than that on other seven kinases examined, although it was reported to be much higher,
Sequence of DNA-PKcs revealed its similarity to phosphatidylinositol 3-kinase (PI3K) (Hartley et al., 1995). This study also that fungal metabolite wortmannin (Fig.2 B), which had been known as an inhibitor of PI3K, could inhibit DNA-PK (Hartley et al., 1995). IC50 of wortmannin is reported to be 0.016 µM and 0.12 µM (Sarkaria et al., 1998; Izzard et al., 1999). It was also shown that wortmannin binds covalently to DNA-PKcs and functions as non-competitive, irreversible inhibitor of DNA-PK (Sarkaria et al., 1998; Izzard et al., 1999). Expectedly, a number of studies have demonstrated radiosensitizing effects of wortmannin but there is a concern whether the observed radiosensitization was really due to inhibition of DNA-PK. In this regard, some studies showed that radiosensitization by these compounds could be observed even in DNA-PKcs-deficient cells (Rosenzweig, et al., 1997; Hosoi et al., 1998), indicating that radiosensitization by these compounds was not solely due to inhibition of DNA-PK. In addition to ATM discussed next, PI3K-Akt pathway, which might be even more sensitive to wortmannin, might be important to sustain cell survival after irradiation. On the other hand, there are also studies showing that radiosensitization was not observed in DNA-PKcs deficient cells (Chernikova, et al., 1999; Hashimoto, et al., 2003). These studies argue that, even if wortmannin affect PI3K or other kinase more potently than DNA-PK, the radiosensitizing effect might be mainly due to inhibition of DNA-PK.
Another PI3K inhibitor LY294002, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (Fig.2 C) was also shown to inhibit DNA-PK. In contrast to wortmannin, LY294002 competes with ATP (Izzard et al., 1999). IC50 of LY294002 is reported to be 6 µM (Izzard et al., 1999). LY294002 was used as a leading compound to explore more potent and selective inhibitors of DNA-PK. NU7026, 2-(morpholin-4-yl)-benzo[h]chromen4-one (Fig.2 D), was found as selective inhibitor of DNA-PK (Veuger et al., 2003). IC50 of NU7026 was 0.23 µM for DNA-PK, 13 µM for PI3K and >100 µM for ATM and ATR (Veuger et al., 2003). NU7026 sensitized cultured cells toward radiation in a manner dependent on DNA-PK (Veuger et al., 2003). Synthesis and screening of chromen-4-one library resulted in identification of NU7441 (Fig.2 E), 8-dibenzothiophen-4-yl- 2-morpholin-4-yl-chromen-4-one (Leahy et al., 2004; Hardcastle et al., 2005). IC50 of NU7441 was 0.014 µM for DNA-PK, 5.0 µM for PI3K and >100 µM for ATM and ATR (Leahy et al., 2004). NU7441 sensitized cultured cells toward radiation and etoposide in a manner dependent on DNA-PK at 0.5 µM (Zhao et al., 2006). Screening of the derivatives of LY294002 also lead to the identification of other selective inhibitors of DNA-PK; IC60211 (Fig.2 F, 2-Hydroxy-4-morpholin-4-yl-benzaldehyde, IC50: 0.43 µM), IC86621 (Fig.2 G, 1-(2-Hydroxy-4-morpholin-4-yl-phenyl) -ethanone, IC50: 0.12 - 0.17 µM), AMA37 (Fig.2 H, 1-(2-Hydroxy- 4-morpholin-4-yl-phenyl)-phenyl-methanone, IC50: 0.27 µM) (Kashishian et al., 2003; Knight et al., 2004).
It was recently reported that NVP-BEZ235 (Fig.2 I, 2-methyl-2-(4-(3-methyl-2-oxo-8-(quinolin-3-yl)-2,3-dihydro-1H -imidazo[4,5-c]quinolin-1-yl)phenyl)propanenitrile), which had been initially identified as a dual inhibitor for PI3K and mammalian target of rapamycin (mTOR) (Maira et al., 2008), inhibited DNA-PK, ATM and ATR and sensitizes cells to ionizing radiation (Toledo et al., 2011; Mukherjee et al., 2012). NVP-BEZ235 sensitized the cultured cells to radiation and inhibited DSB repair, as shown by persistence of 53BP1 foci, to a greater extent than NU7026 and KU55933 (Mukherjee et al., 2012). NVP-BEZ235 sensitized ATM-deficient cells, i.e., fibroblast from ataxia telangiectasia patient, and also DNA-PKcs-deficient human glioma cell M059J (Mukherjee et al., 2012), which could be due to dual inhibition of DNA-PK and ATM. Moreover, inhibition of
Screening of a three-substituted indoline-2-one library lead to identification of SU11752 (Fig.2 J) as selective DNA-PK inhibitor (IC50: 0.13 µM) (Ismail, et al., 2004). Vanillin, 4-hydroxy-3-methoxybenzoaldehyde (Fig.2 K), was found to inhibit DNA-PK albeit at a relatively high concentration, i.e., IC50 1500 µM (Durant and Karran, 2003). Screening of library of vanillin derivatives lead to finding of more potent inhibitors, 4,5-dimethoxy-2-nitrobenzaldehyde (DMNB, Fig.2 L) and 2-bromo-4,5- dimethoxybenzaldehyde (Fig.2 M), whose IC50 were 15 µM and 30 µM, respectively (Durant and Karran, 2003).
DNA-PK can be inhibited by homopolymeric phosphorythioate oligonucleotides, suramin and heparin (Hosoi et al., 2002). Inhibitory activities of homopolymeric phosphorothioate oligonucleotides on DNA-PK were independent of base composition but were dependent on length. IC50 decreased as length increased: 0.975 µM for 12 mer and 0.013 µM for 36 mer (Hosoi et al., 2002). IC50 of suramin and heparin were 1.7 µM and 0.27 µg ml-1, respectively (Hosoi et al., 2002). Suramin sensitized cultured human cancer cell toward ionizing radiation but not to ultraviolet radiation (Hosoi et al., 2004). Furthermore, suramin did not affect the radiation sensitivity of
Single chain antibody variable fragment (scFv) is another approach to achieve specific inhibition of DNA-PK. ScFv was initially generated from existing murine monoclonal antibody 18-2, expressed in
ATM, ataxia-telangiectasia mutated, was identified as the gene responsible for the genetic disorder ataxia telangiectasia, showing similarity to PI3K (Savitsky et al., 1995). Subsequently similarity between ATM and DNA-PKcs, suggesting ATM might also be a protein kinase rather than a lipid kinase (Hartley et al., 1995). ATM was shown to be a protein kinase, which is activated by DNA damage and phosphorylates p53 at Ser15 (Banin et al., 1998; Canman et al., 1998).
Like DNA-PK, ATM was also shown to be inhibited by wortmannin with IC50 of 0.15 µM (Sarkaria et al., 1998). It was also shown that administration of wortmannin to cultured cell phenocopies the defect of ataxia telangiectasia cell,
Selective inhibitors were found from the small molecule library of LY294002 derivatives (Hickson, et al., 2004; Hollick et al., 2007). Among them KU-55933, 2-morpholin-4-yl-6-thialanthren-1-yl-pyran-4-one (Fig.3 B) showed inhibition of ATM with IC50 of 0.013 µM (Hickson, et al., 2004). IC50 values for other PI3K-related kinases were greater than 1.8 µM, which is approximately 200-fold higher than that for ATM (Hickson, et al., 2004). As in the case of DNA-PK inhibitors, morpholine group is important for inhibitory activity, as KU-58050, in which morpholine group was replaced by piperidine group was much less effective: IC50 was 300 µM (Hickson, et al., 2004). KU-55933 inhibited
Modification of KU-55933 lead to identification of KU-60019 (Fig.3 C), 2-((2R, 6S)-2, 6-Dimethyl-morpholin-4-yl)-N-[5-(6-morpholin-4- yl-4-oxo-4H-pyran-2-yl)-9H-thioxanthen-2-yl]-acetamideas a more potent inhibitor of ATM (Golding et al., 2009). IC50 of KU-60019 for ATM was 0.0063 µM, whereas IC50 values for DNA-PKcs and ATR were 1.7 µM and >10 µM, respectively (Golding et al., 2009). KU-60019 mostly abolished ionizing radiation-induced phosphorylation of p53 at Ser15 and Chk2 at Thr68 at 1 to 3 µM, whereas > 10 µM concentration of KU-55933 was required to obtain similar extent of inhibition (Golding et al., 2009). KU-60019 at 1 µM showed similar extent of radiosensitization to KU-55933 at 10 µM (Golding et al., 2009).
Independent screening of chemical library lead to identification of CP466722, 2-(6,7-dimethoxyquinazolin-4-yl)-5- (pyridin-2-yl)-2H-1,2,4-triazole-3-amine, as a novel inhibitor of ATM (Rainey, et al., 2008). CPP466722 inhibited in cellolo phoshorylation of ATM at Ser1981, SMC1 at Ser957 and Chk2 at Thr68 but not affected the phosphorylation events, which are thought to be mediated through other PI3K-related kinases (Rainey, et al., 2008). CPP466722 sensitized cultured cells to radiation to a similar extent to KU-55933 (Rainey, et al., 2008).
CGK733 was reported to be a dual inhibitor of ATM and ATR, but the report was retracted thereafter because of fabrication. Even after the retraction, CGK733 was marketed as an inhibitor of ATM and ATR and several studies used CGK733 to show the involvement of ATM and/or ATR in response to DNA damage caused by a variety of agents. On the other hand, however, there is a report that this compound did not affect ATM and ATR kinase as shown, respectively, by ionizing radiation-induced phosphorylation of ATM at Ser1981 and Chk2 at Thr 68 and by ultraviolet radiation-induced phosphorylation of Chk1 at Ser317 (Choi et al., 2011, and references therein).
ATR was initially identified as a molecule structurally related to human ATM and yeast Rad3 (Cimprich et al, 1996; Keegan et al., 1996). ATR was then shown to be a protein kinase, which is capable of phosphorylating itself and p53 at Ser15 (Canman et al., 1998). ATR is thought to be a sensor of single-stranded DNA (ssDNA), binding to RPA (Replication Protein A) via ATRIP (ATR-interacting protein) (Zou and Elledge, 2003).
Despite of its structural similarity to DNA-PKcs, ATM and PI3K, ATR appeared refractory to wortmannin inhibition: IC50 of wortmannin for ATR was 1.8 µM, which was 10- to 100-fold higher than that for DNA-PKcs and ATM (Sarkaria et al., 1998). Selective inhibitors of ATR emerged recently.
Schisandrin B is an active ingredient of
Library of 623 compounds, which had exhibited some inhibitory effects on PI3K, was screened for their effects on
High throughput screening of ATR by
NU6027, 2,6-diamino-4-cyclohexyl-methyloxy-5-nitroso-pyrimidine, was initially developed as an inhibitor of cyclin- dependent kinases (CDKs) (Arris et al, 2000). NU6027 was recently found, however, to inhibit ATR more potently than CDK2 (Peasland et al., 2011). NU6027 inhibited
It might be added that p53-deficient cells, than p53-proficient cells, exhibited greater extent of sensitization toward ionizing radiation and other DNA damaging agents by ATR inhibitors NVP-BEZ235, ETP-46464 (Toledo et al., 2011), VE-821 (Reaper et al., 2011) and NU6027 (Peasland et al., 2011). This could be due to simultaneous inactivation of two checkpoint pathways mediated through ATM and ATR, respectively, the former of which involves p53. As most of cancer cells lose p53 function, inhibition of ATR might be a promising approach to achieve selective killing of cancer cells, minimizing the effects to surrounding normal cells.
3. Other DSB repair enzymes as targets for radiosensitizer
3.1. MRN complex
Mirin, Z-5-(4-hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one (Fig.5), was identified in a screen for smalll molecules inhibiting MRN-ATM pathway (Dupre et al., 2009). Restriction enzyme-digested plasmid was added to cell-free extract prepared from
3.2. DNA ligase IV
Inhibitors of DNA ligases were searched in a database of 1.5 million commercially available low molecular weight chemicals by computer-aided drug design approach based on crystal structure of DNA ligase I (Chen et al., 2008). In this approach, L82 ((E)-2-((2-(2-((3,5-dibromo-4-methylphenyl)amino)ethyl)hydrazono)methyl)-4-nitrophenol, Fig.6 A), inhibiting DNA ligase I, L67 ((E)-4-chloro-5-(2-(4-hydroxy-3-nitrobenzylidene)hydrazinyl)pyridazin-3(2H)-one, Fig.6 B), inhibiting DNA ligases I and III, and L189 ((E)-6-amino-5-(benzylideneamino)-2-mercaptopyrimidin-4-ol, Fig.6 C), inhibiting DNA ligases I, III and IV, were identified. None of them inhibited the activity of T4 ligase (Chen et al., 2008). Kinetic alalysis indicated that, whereas L82 is non-competitive inhibitor, L67 and L189 competes with DNA substrate (Chen et al., 2008). L67 sensitized cultured human cancer cells to methylmethansulfonate at 3 µM (Chen et al., 2008). Similarly, L189 sensitized cultured human cancer cells to ionizing radiation at 20 µM (Chen et al., 2008). It might be noted that the sensitizing effects of L67 and L189 were not observed in non-cancer cells, suggesting is selective effects on cancer cells (Chen et al., 2008). These compounds can be a leading compounds for the development of more potent and/or more selective inhibitors of DNA ligases.
3.3. DPYD as a new target
Gimeracil, 5-chloro-2,4-dihydroxypyridine (Fig.7), is an inhibitor of dihydropyrimidine dehydrogenease (DPYD) and used as a component of oral anti-cancer medicine S-1, in order to suppress degradation of 5-fluorouracil. The results of clinical trial of concurrent chemoradiotherapy using S-1 suggested possible radiosensitizing effect of S-1. Gimeracil increased radiosensitivity of cultured human cancer cells of various origin within 200 – 5,000 µM, being maximal within 1,000 – 5,000 µM range (Takagi et al., 2010). Cell lines deficient for DNA-PKcs or Ku86 were sensitized by gimeracil to radiation even to a greater extent than respective control cells (Takagi et al., 2010). On the other hand, radiosensitiztion was not observed in cell lines deficient for XRCC3, NBS1 or FANCD2 (Takagi et al., 2010). These observations collectively suggested that gimeracil exert radiosensitizing effects through inhibition of HR-mediated DSB repair. Gimeracil reduced the frequency of homologous recombination of chromosomal substrate including the restriction site of I-SceI by approximately 15% (Takagi et al., 2010). Gimeracil reduced the formation of ionizing radiation-induced foci of Rad51 and RPA but increased that of Nbs1, Mre11, Rad50 and FancD2 (Sakata et al., 2011). This observation suggested that gimeracil might have inhibited the step after strand resection by Mre11-Rad50-Nbs1 complex but before the loading of RPA and Rad51 onto single-stranded DNA. Although the role of DPYD in HR has not been described, treatment with siRNA for DPYD sensitized cells to ionizing radiation to a similar extent to gimeracil and also diminished the radiosensitization by gimeracil (Sakata et al., 2011). These results collectively indicate that gimeracil exerts radiosensitizing effects through inhibition of DPYD, which might have a novel role in HR.
4. Radiosensitization by hyperthermia
Hyperthermia, heating parts of body at 40 - 45 oC, has been used to treat cancer mostly combined with ionizing radiation. Hyperthemia is known to sensitize cells to ionizing radiation, inhibiting the repair of DNA damages including DSBs, but the molecular mechanism of radiosensitization by hyperthermia has remaind to be clarified.
Effects of hyperthermia on DNA polymerases α and β have been studied for a long time. These studies suggested that DNA polymerase β was sensitive to hyperthermia and its inactivation was correlated to radiosensitization as well as to cell killing (Spiro et al., 1982). Later it was reported that DNA polymerase β knocked out cells or overexpressed cells exhibited radiosensitization by hyperthermia indifferent from control cells (Raaphorst et al., 2004). Elucidation of DSB repair mechanisms through NHEJ and HR provided clues to the mechanisms of radiosensitization by hyperthermia.
Among essential factors in NHEJ, Ku is shown to be affected by hypethermia. Purified DNA-PK lost its activity upon incubation at 44°C for 5 - 30 min (Matsumoto et al., 1997). When DNA-PKcs and Ku were heated separately, heating of Ku, but not DNA-PKcs, lead to decrease in DNA-PK activity, suggesting that Ku, rather than DNA-PKcs, is heat sensitive component (Matsumoto et al., 1997). Inactivation of DNA-PK activity by hyperthermia was observed also
There is also accumulating studies on the effects of hyperthermia on MRN complex. It was initially found that Mre11, Rad50 and Nbs1 exported from nucleus to cytoplasm upon hyperthemia at 42.5°C or 45.5°C (Zhu et al., 2001; Seno and Dynlacht, 2004). This nuclear export of MRN complex increased when cells were irradiated prior to hyperthermia (Zhu et al., 2001; Seno and Dynlacht, 2004). Similar phonomenon was observed in mild hyperthermia at 41.1°C (Xu et al., 2002). However, in a recent study, inhibition of nuclear export of MRN complex by leptomycin B did not diminish radiosensitization by hyperthermia at 45.5°C for 10 min (Dynlacht et al., 2011). It was also shown, nevertheless, that ATLD cells, which have mutated in Mre11, did not show radiosensitization by hyperthermia at 41.5°C for 2 hrs or at 45.5°C for 10 min (Dynlacht et al., 2011). On the other hand, radiosensitization by hyperthermia was observed in NBS cells and Rad50-knocked down cells (Dynlacht et al., 2011). Exonuclease activity of Mre11 was decreased to ~10% by 42.5°C treatment for 15 min (Dynlacht et al., 2011). These results collectively indicate Mre11 as target for radiosensitization by hyperthermia.
Hyperthermia is shown to affect BRCA1 and BRCA2. Heating cultured human cancer cells at 42°C for 1 - 2 hrs or more decreased the amount of BRCA1 (Ma et al., 2003). It might be caused by protein degradation, but various inhibitors of proteases, so far as tested, failed to suppress the decrease of BRCA1 (Ma et al., 2003). Alternatively, it might be caused by protein aggregation and reduced solubility in aqueous buffers. It was also shown that BRCA1 deficient cells were sensitive to hyperthermia (Ma et al., 2003). Recent study reported the degradation of BRCA2 induced by mild hyperthermia at 41°C to 42.5°C (Krawczyk et al., 2011). Rad54-deficient ES cells and cells treated with XRCC3 siRNA were not radiosensitized by mild hyperthermia (Krawczyk et al., 2011). Furthermore, mild hyperthermia showed synthetic lethality with PARP-1 inhibitor oraparib, like BRCA2 deficient cancer cells (Krawczyk et al., 2011). These data collectively indicated BRCA2 as a major target of mild hyperthermia.
Obviously, hyperthermia inactivates many enzymes and induces aggregation of many proteins. In this regard, hyperthermia is not specific on certain enzyme, unlike inhibitors described above. However, susceptiblity to inactivation by hyperthermia might be greatly different among proteins. The extent of radiosensitization by hyperthermia can be greatly infuluenced by many factors,
5. Concluding remarks and future perspectives
Because of great advances in our understanding of the moleuclar mechanisms of DSB repair in past two decades, extensive studies have been done to achieve radiosensitization by modification of DSB repair molecules. Especially, a number of inhibitors have been developed for DNA-PK, ATM and ATR protein kinases. We saw here that preceeding studies on DNA-PK and on PI3K greatly facilitated the studies on ATM and ATR. It might be underscored that LY294002, preexisting inhibitor of PI3K, served as a leading compound and enabled the finding of potent and specific inhibitors like NU7441 and KU-55933.
Studies toward the clinical application of these compounds are underway. Preclinical studies of pharmacokinetics and metabolism in mice were conducted for NU7026 and NU7441. In the case of NU7026, the radiosensitizing effect on cultured cancer cell was marginal upon the treatment at 10 µM for 2 hrs and could be increased by extending the treatment time up to 24 hrs (Nutley et al., 2005). On the other hand, however, NU7026 underwent rapid plasma clearance in mice, presumably because of oxidation and ring opening of morpholino group (Nutley et al., 2005). It was estimated that NU7026 should be administered four times per day at 100 mg/kg intraperitoneally in order to obtain radiosensitization (Nutley et al., 2005). In the case of NU7441, the radiosensitizating effect on cultured cancer cell could be obtained by treatment at 1 µM for 1 hr (Zhao et al., 2006). The concentration of NU7441 required for radiosensitization could be maintained within tumor tissues for more than 4 hrs at nontoxic dose (Zhao et al., 2006). The administration of etoposide and NU7441 to mice bearing human tumor xenografts synergistically delayed tumor growth, indicating the chemosensitizing effect of NU7441
Search for inhibitors of enzymes other than protein kinases has been difficult due to the absence of assay system suitable for highthroughput screening. However, inhibitors of other enzymes,
In addition to use of each chemicals alone, use of two or more chemicals together to inhibit two pathways of DSB repair or one of them with other repair mechanisms, which is called synthetic lethality approach, will be promising. Successful example is shown in the treatment of cancers arisen in the carriers of BRCA2 mutation with PARP-1 inhibitors. When PARP-1, which is essential for single-strand break (SSB) repair, is inactivated, SSB is converted to DSB, which requires BRCA2 to be repaired. As BRCA2 mutation is heterozygotic, normal cells retain BRCA2 function. On the other hand cancer cells have lost BRCA2 function and, therefore, shows extreme sensitivity to increased sensitivity to converted DSBs. This is instructive also to find a means to discriminate cancer cells and normal cells. As described in the previous section, although hyperthemia is not an approach to target a certain molecule specifically, it did show synthetic lethal effects with PARP-1 inhibitor. These examples underscores the importance of the choice of agents based on the thorough consideration of biological characteristics and genetic background of each cancer and patient. In addition to continuing persuit for the new radiosensitizing agents, extensive studies would be necessary regarding combinatorial approach and personalized medicine.
We apologize for not citing many important literatures because of space limitation.
Our study was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sport, Science and Technology of Japan to YM. MKS is supported by Takeda Science Foundation, Japan Society for Promotion of Sciences and Tokyo Biochemistry Research Foundation.