Pollution of soils, sediments, and groundwater is a matter of concern at global level. Industrial waste effluents have damaged several environments; thus, pollutant removal has become a priority worldwide. Currently, bioremediation has emerged as an effective solution for these problems, and, indeed, the use of haloarchaea in bioremediation has been tested successfully. A bibliographic review is here presented to show the recent advances in bioremediation of polluted soil and wastewater using haloarchaea. Several aspects related to the publications in the context of bioremediation and the innovative proposal of using haloarchaea are also analyzed. The results obtained claim that most of the countries show an alarming contamination issue, which focuses the finance into research about environmental friendly remediation approaches to solve this problem. Concerning bioremediation, strategies to treat soils and polluted waters have been much more studied than bioremediation processes addressed to industrial residues. Publications about bioremediation mainly comes from USA, China, and India at the time of writing this work. However, works using haloarchaea in bioremediation came from Kuwait and Spain. Haloarchaea have been investigated as a potential tool for industrial and environmental purposes. Further research is needed to elucidate the optimal growth conditions and environmental parameters for this proposal.
- Haloferax mediterranei
- heavy metals
- green biotechnology
Last decades highlight the importance of the increasing water demand, the environmental pollution or the presence of excess nitrogen in the environment, causing serious alterations in soil, water, and atmosphere [1, 2]. The presence of heavy metals in the environment as well as the C:N ratio has been a major problem in soils and waters due to their toxicity; therefore, their elimination before being released into the media is relevant from environmental, economic, and social points of view . There are many methods to remove pollutants, involving physical, chemical, or biological approaches. During recent decades, the latter has received an increasing attention as a way of removing or neutralizing pollutants from a contaminated site using native or introduced microorganisms .
One of those biological strategies recently applied is the bioremediation, which is defined as the application of the metabolic capabilities of bacteria, fungi, yeast, algae, plants, and microbial mats to degrade harmful contaminants to living organisms or transform them into less toxic compounds by naturally enhancing degradation processes [3, 5]. Two types of bioremediation might be found according to the way of use: (i) intrinsic in situ bioremediation: natural degradation of remaining contaminants in the environment, without any external intervention; (ii) engineered in situ bioremediation: requires an external supply to increase the biodegradation rate by accelerating the growth of further organisms through the secretion of essential nutrients. In most of the situations, engineered bioremediation prevails over the intrinsic one except for those cases in which the natural occurrence of biodegradation is faster than the migration .
Engineered bioremediation includes two approaches: biostimulation and bioaugmentation. The addition of limiting nutrients to a contaminated environment where all the necessary natural microorganisms are assumed to be present refers to biostimulation. Bioaugmentation controls the quantity of microorganisms with the desired catalytic capability needed for biodegradation of specific compounds [7, 8]. A combination between these disciplines is suggested to be a promising strategy to speedup bioremediation . Nowadays, biological remediation is considered one of the best options for the treatment of contaminated environments  due to their numerous advantages such as in situ treatment of soils or waters, high efficiency, and no secondary pollution [5, 9].
1.1. Microorganisms and bioremediation: the case of haloarchaea
Microorganisms are the ones which oversee the bioremediation process, thanks to their ability of using environmental contaminants as source of carbon and energy and their small contact surface . Prokaryotes are the main biotechnological agents used for biodegradation of organic matter, although fungi, algae, and protozoa can be used for the same purpose but affecting differently the nature of the compound for the degradation . In addition, phytoremediation has been for a long time as a promising technique to degrade contaminants from soil sediment, surface, and groundwater [11, 12]. It was proposed the use of microbial consortium, such as phosphate solubilizing microbes, in association with phytoremediation to achieve a most trustworthy approach for the enhancement of the remediation of metal-contaminated soils .
More recently, some research has shown that archaea can be of high interest for biotechnological purposes including bioremediation. Archaea were first characterized as a group of single-celled prokaryotic microorganisms living in extremophile environments with elevated temperatures (thermophiles), low or high pH (acidophiles, alkalophiles), high salinity (halophiles), or strict anoxia . The ability of those species to grow under a wide range of extreme conditions equally to their mechanisms supporting genetic plasticity  make them good candidates for research in different fields, including biotechnology and, consequently, bioremediation [9, 14, 16].
Nowadays, there is an increasing interest in the optimization of bioremediation approaches in high salt environments, which are mostly influenced by the discharge of industrial effluents [2, 17]. The increase of salinity and nitrogen compounds in soils and ground waters during the last years is focusing attention in the physiological and molecular mechanisms involved in salt-stress tolerance and nitrate metabolism . Conventional microbiological processes are not capable of being executed at high salt concentrations, indicating that microbial bioremediation of hypersaline produced water requires halophiles [19, 20].
In this sense, haloarchaea have been successfully tested for biotechnological applications throughout the last decade [16, 21, 22]. This is because of the extreme properties of their enzymes: high thermostability and resistance to denaturing agents such as detergents, organic solvents, and extreme pH . Moreover, their proteins are rich in acidic amino acids, which allow the maintenance of the stable conformation and activity at high salt concentrations .
Most of the species from Halobacteriaceae and Haloferacaceae families can grow under anaerobic conditions, and they are more abundant and diverse than other microbes in environments containing increasing salt concentrations (more than 20–25% salts) [25, 26]. They are found in natural salty lakes and saltmarshes created by human beings, which are extremely alkalinized places, likewise in submarine brine pools and brine pockets within sea ice [23, 27].
General biochemical pathways related to nitrogen cycle, heavy metals, hydrocarbons, or aromatic compounds have been described in haloarchaea as efficient pathways. Consequently, they might be applied for bioremediation proposals in saline and hypersaline wastewater treatments, thanks to their high tolerance to salt, metals, and organic pollutants [3, 14, 25].
1.2. Haloferax mediterranei: a good model for viable bioremediation applications
Hfx. mediterranei is a haloarchaeon from the family Haloferacaceae , first isolated from seawater evaporation ponds near Alicante, Spain . It can grow in a broad range of NaCl concentrations (1.0–5.2 M) , thanks to its remarkable metabolic efficiency and genome stability at high salt concentrations [25, 30].
Hfx. mediterranei is also a denitrifying haloarchaeon based on its ability to reduce nitrate and nitrite under oxic and anoxic conditions [31, 32]. In the presence of oxygen, this haloarchaeon takes profit of nitrate and nitrite as nitrogen sources for its own proliferation  through assimilation pathway. Under oxygen depletion, it uses both ions as terminal electron acceptors in the respiratory chain via denitrification [32, 34]. Once nitrogenous compounds are consumed, brines become free of nitrogenous compounds such as nitrate, nitrite, or ammonium.
Owing to this specific metabolism, it was demonstrated that Hfx. mediterranei was capable of removing most of the nitrogen compounds present in a treated medium, specifically in anoxic conditions after the induction of the denitrification pathway . Its resistance to very high nitrate (up to 2 M) and nitrite (up to 50 mM) concentrations makes them the highest described from a prokaryotic microorganism [3, 35]. Furthermore, the respiratory nitrate reductase of this organism can reduce efficiently bromate and (per)chlorate thanks to the use of these compounds as terminal electron acceptors .
Because of its capability of growing faster than most of other members of the same family and their described characteristics, Hfx. mediterranei has served as a good model for haloarchaeal physiology and metabolic studies during several decades [30, 37]. Therefore, it represents a highly interesting model for research in potential development of bioremediation approaches in polluted soils, sediments, and waters.
We had reviewed on bioremediation of polluted salty soils and water using haloarchaea in our previous manuscript [3, 22, 25, 34, 36] and had introduced the importance of haloarchaea as a bioremediation tool. Thus, in this manuscript, we statistically investigated the recent trend of the study and estimated the characteristics.
2. Research method
The starting point for this work was the bibliographic-bibliometric review about the most interesting keywords related to the topic. To optimize the search of information, it was used “PubMed” (
The combination between these keywords was used to optimize the search for publications. All the keywords were grouped into two subclasses: the first one includes the terms “archaea,” “bioremediation,” “haloarchaea,” and “halophilic archaea”; the second class involves “contaminated soil,” “industrial raw,” “industrial waste,” “salted soil,” “salty soil,” and “wastewater.” These classes of keywords and their synonyms were crossed, obtaining a total pool of 27 concepts: “archaea and contaminated soil,” “archaea and bioremediation,” “archaea and industrial raw,” “archaea and industrial waste,” “archaea and salted soil,” “archaea and salty soil,” “archaea and wastewater,” “bioremediation and contaminated soil,” “bioremediation and haloarchaea,” “bioremediation and halophilic archaea,” “bioremediation and industrial raw,” “bioremediation and industrial waste,” “bioremediation and salted soil,” “bioremediation and salty soil,” “bioremediation and wastewater,” “haloarchaea and contaminated soil,” “haloarchaea and industrial raw,” “haloarchaea and industrial waste,” “haloarchaea and salted soil,” “haloarchaea and salty soil,” “haloarchaea and wastewater,” “halophilic archaea and contaminated soil,” “halophilic archaea and industrial raw,” “halophilic archaea and industrial waste,” “halophilic archaea and salted soil,” “halophilic archaea and salty soil,” and “halophilic archaea and wastewater.” The following fields were selected in the databases to delimit the results: “article title,” “abstract,” and “keywords.”
In April 2017, the search about number of publications per year was finished, using a range of 27 years (1990–2016). They were obtained regarding the following items: country, type of document, language, and publication date. The language and type of document were not indispensable for analyzing purposes. The research question was: what is the impact of haloarchaeal applications in bioremediation in research/science?
To assess the differences in the search of publications between the three mentioned databases, the analysis of variance (ANOVA) was performed using R-project statistics. ANOVA statistics was chosen, since it is usually employed for this kind of studies. The significant threshold for these analyses had a p value of 0.05 except for those cases where the transformation of the data was not efficient, and it was needed to change it (p = 0.01). In those cases where at least one of the levels was statistically different, it was performed a test to delimit the class which is different from the rest or differences between classes. The test used was Tukey’s HSD. For the average calculations and graphical representations, Microsoft Excel 2016 was employed.
3.1. Analysis of databases results regarding publications per year
First, a contrast between PubMed, Scopus, and Web of Science was performed regarding the number of publications per year. The purpose of this approach was to study if there were significant differences in the search concerning the database based on the keywords selected. To summarize the data, only publications from a range of years (1990–2016) were used in the statistical test of the total 10 keywords, including synonyms. The year 2017 was not considered in this interval due to the inaccuracy of the results and the unpredictable number of total publications at the end of the year.
An ANOVA test was performed to analyze the differences between the three databases (PubMed, Scopus, and Web of Science) and to compare them looking for statistical significance in the number of publications. The requirements for ANOVA test were corroborated as follows: independence of the data, normality of the results, and homogeneity of the variances. ANOVA statistics was done obtaining that at least one of the databases was different (p < 0.05), so Tukey’s test was performed to distinguish the variable class by comparing each level with the rest (Figure 1). Concerning the p value of the results, there are only significant differences between Web of Science and PubMed as it is displayed in Figure 1 by means of lines referring to similarities. The differences between the databases are remarked in the way PubMed is significantly different from Web of Science (p = 0.0018) and not for Scopus (p = 0.39). No significant differences were documented between Scopus and Web of Science regarding the number of total publications per year (p = 0.07). PubMed, as a service from the US National Library of Medicine (NLM), carries the highest number of publications. However, Web of Science is one of the most used search database, containing quality publications from editorials with high impact factor.
3.2. Study of the number of publications according to continents and countries
The location of the research related to the topic keywords according to continents and countries was also analyzed. Most of the countries in the world possess almost one publication along time. However, United States of America (USA) and China present the highest number of publications (111,392 and 89,849, respectively). The whole subset of countries with representativeness in the research about bioremediation using haloarchaea in soils, waters, and industrial raw is shown in Figure 2, with different intensities according to the abundance of publications. The language was not a representative deal since almost all the publications were written in English (97.4%). The diversity of the language in the different countries is remarkable despite of this, where other writing languages are German (0.5%), Spanish (0.4%), and Portuguese (0.4%).
As a further matter, all the data from the number of publications per country were grouped into continents for a more general view of the representativeness. The following results were obtained concerning total publications in the search: Asia (210,850), Europe (210,086), America (165,946), Oceania (19,038), Eurasia (18,752), and Africa (18,591). Africa has the lowest value of publications although the abundance of countries with positive results is large. Eurasia includes Turkey, Russia, Kazakhstan, and Georgia, which can be considered from Europe and Asia due to their geographical localization. Instead of including these countries on a single continent, a different class of continent is considered due to their high values of publications. The final values of Europe and Asia are so close with a difference of 800 publications, which implies a possible change of dominance in the next few years.
3.3. Impact of bioremediation using haloarchaea in literature
Haloarchaea were not directly related to bioremediation in literature until year 2007, and, even after this year, the number of publications has been low as can be noted in the percentage of occurrence of haloarchaea in bioremediation (0.06%). This value was calculated by analyzing the total publications per year regarding “bioremediation” as keyword in comparison to the combination of “bioremediation and haloarchaea or halophilic archaea.”
The main lines of investigation regarding bioremediation using haloarchaea are developed by Bonete MJ (University of Alicante), Al-Mailem DM (University of Kuwait), and Martínez-Espinosa RM (University of Alicante). Furthermore, University of Alicante, University of Kuwait, and University of East Anglia are the most important institutions in terms of developing research lines about haloarchaeal species applied in bioremediation. These affiliations may be duplicated due to the fact some publications can contain two or more institutions in collaboration. Many of the authors with active experimental approaches in this way belong to the University of Alicante in collaboration with University of East Anglia (approximately, 50% of publications from the University of Alicante are in collaboration; 100% of publications from University of East Anglia are made in collaboration with University of Alicante).
Besides, the influence of the keywords “bioremediation” and “haloarchaea or halophilic archaea” was analyzed to delimit the total number of publications per year in the chosen databases. These results are plotted in Figure 3. There is an increasing tendency of the publications, with higher quantities of results during last years both in bioremediation (Figure 3A) and haloarchaea (Figure 3B). The keyword “halophilic archaea” shows a higher representativeness than “haloarchaea” in the search due to the presence of the word “halophilic” which can be used for referring other species different from archaea (bacteria, microorganism, and plant; Figure 3B).
Both “bioremediation and haloarchaea” and “halophilic archaea” publications present a lower value of results (Figure 4). Since “haloarchaea” have a lower representativeness in literature than “bioremediation,” the results show a limitation in the number of publications per year. Increasing results appear since year 2007, although some publications have been documented before in the way of potential studies using haloarchaea. Below year 2005, no guaranteed results concerning the topic are shown due to the lower interest in haloarchaea and the probable appearance of other microorganisms because of the use of “halophilic” in the search.
The impact of bioremediation research by countries is shown in Figure 5. This representation remarks the abundance of publications in the top 10 countries, where the USA is the dominant followed by China and India. This is not surprising owing to the presence of those countries in the top three of publications regarding all the keywords. In Figure 6, the top 10 countries that investigate in bioremediation using haloarchaea are shown, with Kuwait and Spain dominating the volume of total publications. Although Kuwait is not on the top 10 countries in bioremediation publications, it is important to highlight its predominance in the study of haloarchaea as bioremediation tool.
Several areas of Kuwait are mainly characterized by hypersaline environments. Due to this fact, desalination is sometimes the only way of reliable water supply in most of the Arabian Gulf states. In South Kuwait, a desalination plant has showed a continued increase of salt, which can affect the local marine biodiversity. In 2011, it was suggested a distinct water quality approach to detect those issues without affecting the marine environment surrounding the water plant . Desalination plants manage a huge quantity of salty water, although they imply several negative impacts. The main cause of desalination plant pollution in the environment includes noise pollution, visual pollution, reduction in recreational fishing and swimming areas, emission of materials into the atmosphere, and brine discharge . Likewise, the climate is very like the one in Spain.
3.4. Bibliographic review of Haloferax mediterranei
Between years 2013 and 2016, there was an increase of interest for the study of this haloarchaeon and their possible applications. The highest number of publications concerning Hfx. mediterranei comes from Spain, with descriptions of different type of applications (industrial, environmental or medical) concerning this haloarchaeon. Authors like Bonete and Martínez-Espinosa (University of Alicante) or Pfeifer (Technische Universitat Darmstadt) appear as the first representative authors in this field, developing independent research lines in their institutions. In fact, the University of Alicante (Spain) is the first research institution in the ranking of number of publications about this haloarchaeon (Figure 7). Besides, those authors are studying the biochemistry, molecular biology, and potential applications of Hfx. mediterranei since its discovery. This work is a contribution to that research group, the most involved in the understanding of Hfx. mediterranei in natural contaminated water and further applications [3, 25, 31].
Globally, alternative remediation processes are gaining importance instead of chemical or solid methods in polluted compounds treatment. There is a global concern about wastewater and residue management due to the presence of toxic compounds ; thus, environmental friendly approaches are being financed by those countries with the higher rate of contamination, such as China or India . Bioremediation, biostimulation, and bioaugmentation are suggested to be suitable alternatives and, due to this fact, they have received a great deal of attention in recent years .
Extremophiles may represent a good option for bioremediation of contaminated sites due to their biochemical and molecular properties. Halophilic microorganisms, especially haloarchaea, represent a highly promising way for removing polluted compounds thanks to their effective metabolism under extreme conditions or even in the presence of highly toxic compounds [2, 42]. Previous studies show the potential use of haloarchaea in bioremediation [3, 22]. Thus, a bibliographic review was developed along this work for the sole purpose to show the recent advances in bioremediation using these microorganisms. The recent gain of interest in potential haloarchaeal innovations is also shown. The source of information was based on three databases, some of which present high-quality impact in publications: PubMed, Scopus, and Web of Science. It was surprising the volume of publications from PubMed and Scopus in contrast to Web of Science, a scientific database containing high-quality publications and worldwide used. In fact, Web of Science is considered the most important database in terms of research evaluation of standards in some institutions such as universities, research institutes, or other institutions involved in research management.
Regarding to the topic, several countries are increasing their efforts in developing environmental friendly methods for removing contaminants; thus, the number of publications concerning bioremediation is highly increasing since recent years. The severe contamination documented in China and India may explain the high number of publications in benefit of bioremediation. The need to find solutions to this problem has increased the public and private finance for such projects. Bioremediation might be the solution for soils and water problems of pollution. The concerning about natural water contamination has always been present, but there is an increasing attention of creating soil quality remediation plans in China. Serious pollution risks were confirmed by the Chinese authorities in April 2014. About 16% of soils in China and 34.9% of brownfields exceed national standards for pollution with heavy metals and pesticides . In India, in addition to the drinking water demand, there are evidences of groundwater contamination due to wastes of local industries. People experience symptoms of disease when drinking water from rivers, which reinforce the actual problem of water contamination .
Despite this, the diversity of metabolic pathways of Hfx. mediterranei, and in general haloarchaea, is greater than earlier assumed. The research about biochemical and molecular characteristics of haloarchaea will be a potential issue in biotechnological applications, especially on bioremediation of natural contaminated water. Furthermore, more investigation about the potential use of those microorganisms is needed to elucidate the optimum range of conditions for suitable bioremediation applications.