Anthocyanins are water-soluble pink/red/blue/purple pigments found abundantly in the flesh and skin of fruits, flowers, and roots of different varieties of plants. Compared to vegetative tissues in many plants, fruits have much higher contents of anthocyanins. In general, anthocyanins have antioxidant, anti-inflammatory, antimutagenic, and antiapoptotic activities that benefit human health. To date, anthocyanins in many different fruits have gained intensive studies in structures, biosynthesis, genetics, and genomics. Despite this, difficulties exist in identifying anthocyanins with similar structures and precisely estimating contents within fruit matrices. To improve this challenge, high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) based metabolomics has been shown a powerful technology to distinguish structure-similar anthocyanins. This chapter reviews, summarizes, and discusses the application of HPLC-MS/MS in the annotation or identification of anthocyanins in fruits.
Part of the book: High Performance Liquid Chromatography
Understanding in the evolutionary processes, endangered mechanisms, and adaptive evolution history are key scientific issues in conservation biology. During the past decades, advances in high-throughput sequencing and multi-disciplinary crossover have triggered the development of conservation genomics, which refers to the use of new genomic technologies and genomic information in solving the existing problems in conservation biology. Conservation genomics mainly focuses on the endangered mechanism and conservation strategies aiming at protection of survivability and diversity of endangered species. Application of conservation genomics into the study of endanger plant species has provided innovated protection concept for biologists and promoted the development of population-based conservation strategies. This chapter summarizes the studies of population genomics for agronomically and commercially important plants threatened and endangered, discusses the advantages of conservation genomics for the analysis of genetic diversity, inferences about the history of population dynamics, evaluation of natural forces on wild plant populations, and the establishment of effective conservation strategies. This chapter also presents the development trends in genomics for the conservation of endangered plant species.
Part of the book: Endangered Species