\r\n\tWe accept scientific papers which can be presented as original research papers and review papers. The required length of the full chapters is 10-20 pages and the chapters should be original works (not republished). \r\n\tAs a self-contained collection of scholarly papers, the book will target an audience of practicing researchers, academics, Ph.D. students and other scientists. Since it will be published as an Open Access publication, it will allow unrestricted online access to chapters with no reading or subscription fees.
",isbn:null,printIsbn:"979-953-307-X-X",pdfIsbn:null,doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!1,isSalesforceBook:!1,hash:"a8274c7a57830fae9cfa2dd00780184f",bookSignature:"Associate Prof. Arpit Sand",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/8505.jpg",keywords:"Applications, PEG, Biotechnical, Biomedical, Water Structure, Cell Fusion, DSC Measurement, Phase Diagram, NMR Spectroscopy, Protein Interaction, Grafted PEG Surface, Monte Carlo ,Protein Hybrid Catalyst, Nano Protein ,Metal Complex",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfDimensionsCitations:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 8th 2019",dateEndSecondStepPublish:"September 2nd 2019",dateEndThirdStepPublish:"November 1st 2019",dateEndFourthStepPublish:"January 20th 2020",dateEndFifthStepPublish:"March 20th 2020",remainingDaysToSecondStep:"3 years",secondStepPassed:!0,currentStepOfPublishingProcess:5,editedByType:null,kuFlag:!1,biosketch:null,coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"287032",title:"Associate Prof.",name:"Arpit",middleName:null,surname:"Sand",slug:"arpit-sand",fullName:"Arpit Sand",profilePictureURL:"https://mts.intechopen.com/storage/users/287032/images/system/287032.jpg",biography:"Dr. Arpit Sand is currently an associate professor in the Department of Chemistry, Manav Rachna University, Faridabad, India. He received his BSc in Science and MSc in Chemistry from the University of Allahabad, India, in 2004 and 2006, respectively. He received his Ph.D. in Chemistry from the same university in 2010. Dr. Sand is an editorial board member for Polymer Synthesis Journal by Cambridge Scholars Publishing UK and Journal of Polymer Science. He is an academic book editor and a reviewer for international journals including Carbohydrate Polymers, International Journal of Biological Macromolecules, and Fibers and Polymers, among others.\r\nDr. Sand’s previous roles include assistant professor (guest faculty) in the Department of Chemistry, University of Allahabad; research associate at the National Physical Laboratory (NPL) New Delhi; postdoctoral fellow at Gwangju Institute of Science & Technology (GIST), Korea; postdoctoral fellow at Karolinska Institutet, Sweden; junior researcher at the Brno University of Technology, Czech Republic; and researcher at Soongsil University, South Korea.\r\nHe has more than ten years of teaching and research experience. He is also a life member of the Indian Science Congress and Green Chemistry Network center. He has made significant contributions in the modification and characterization of graft copolymers and films decorated with chalcogenide quantum dots to tune the energy bandgap for solar energy harvesting applications. His research interests include polymer synthesis using different polymerization techniques. 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\n
1. History of continuous beer production
\n
The wish to feel good is the wish to feel good continuously. Transferring this to the consumption of beer may lead to the wish to produce beer continuously (Table 1).
\n
\n
\n
\n
\n
\n\n
\n
Steps in beer production
\n
\n
\n
Process
\n
Aims
\n
Batch
\n
Continuous
\n
\n\n\n
\n
Steeping
\n
Mixing
\n
Steep tank
\n
Screw conveyers
\n
\n
\n
Germination
\n
Mixing
\n
Malting box, tower malting
\n
Worm conveyers
\n
\n
\n
Kilning
\n
Mixing
\n
Floor kiln
\n
Air conveyers
\n
\n
\n
Milling
\n
Mixing
\n
\n
All mills
\n
\n
\n
Mashing
\n
Mixing
\n
Mash tun
\n
Tube heat exchanger
\n
\n
\n
Lautering
\n
Separation
\n
Lauter tun, mash filter
\n
Centrifuge, decanter, Nessie
\n
\n
\n
Wort boiling
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Mixing
\n
Copper
\n
Heat exchanger
\n
\n
\n
Wort clarification
\n
Separation
\n
Whirlpool, flotation
\n
Decanter, centrifuge
\n
\n
\n
Wort aeration
\n
Mixing
\n
Flotation
\n
Inline nozzle
\n
\n
\n
Fermentation
\n
Mixing
\n
Fermentation tub or tank
\n
Immobilised yeast
\n
\n
\n
Maturation
\n
Separation
\n
Storage tank
\n
Centrifuge
\n
\n
\n
Filtration
\n
Separation
\n
Dead-end cave filtration
\n
Crossflow filter
\n
\n
\n
Filling
\n
Avoiding contaminations and oxygen
\n
Tank lorries, kegs, stationary fillers
\n
Rotary-type machines for kegs and bottles
\n
\n\n
Table 1.
Main processes in beer making, their aims and the batchwise and continuous machines.
\n
During the last 100 years, more and more continuous steps have been integrated into the brewing process. Lots of energy can be saved during malting and wort boiling. Filling of the beer needs lots of expensive and labour-intensive machines, which work efficiently, if pursued in a continuous manner.
\n
Brewing processes are mainly mixing and clarification processes. During malting, mashing, wort boiling and fermentation, mixing can easily be performed in a continuous manner. Clarification and separation processes like lautering, wort clarification, yeast settling and maturation are traditionally performed by gravity and settling in a batch process. For the continuous process, these have to be altered into separation processes by decanters, centrifuges and crossflow filtrations. The intersection between a batch process and a continuous process needs buffer vats, tanks before and after the continuous process. Buffers are capacities that cost money, space and energy, have to be cleaned and maintained and limit the advantages of a pure continuous process. In bottling lines conveyer buffers need space before and after each machine, which cost a lot of money and need to be controlled by computers that link the aggregates.
\n
\n
\n
2. Pros and cons of continuous production versus batch process
\n
\n
\n
3. Continuous malting
\n
As malting needs lots of water and energy and only a few malts are produced in big amounts, continuous or semi continuous production is used more often than in brewing (Table 2). This means steeping, germination and kilning during the continuous transportation of the grains [3].
Machinery has less moving parts and less ware parts
\n
\n
\n
\n
\n
Higher degree of automatisation
\n
\n
\n
If things go wrong, it means less stress for the brewers (and yeasts)
\n
\n
\n\n
Table 2.
Continuous brewing processes versus batch processes—the advantages.
\n
In 1960 the Domalt system was built in Toronto (Toronto malting). Barley was transported by water with a pump into a slope malt conditioning screw. The barley is slowly transported upward against rinsing water. Then the barley fell onto an endless filter belt [4]. Water was sprayed on the barley and aerated, and the degree of steeping was adjusted. It fell on a conveyer situated below for the germination. The belt is moved at 0.7 m/h, the green malt being 0.9 m high. Stationary turning machines homogenised the germinating grains. Withering and kilning were also conducted on conveyers by tempered air blowing through the debris. Finally the malt was cooled down by air. Only one person controlled the process. Yields were 1–1.5% above per batch systems. Energy and water were saved [3]. Production size was 11,600 t/a. The steeping and germination time was 100–110 h for two rowed and 70–80 h with multi-rowed barley [4]. Advantages were the quick pregermination at less moisture, the better cytolysis, the shorter time, a bigger kernel volume after the kilning [4], less work labour, better automatisation and less water consumption at higher investment costs.
\n
A Soviet system called Bartnew worked with rotating slanted 1–2° slope long drums. The grains moved 20–60 mm per turn of the drum. In the end the grain had passed every germination stage and was kilned in a vertical kiln with conical chutes [3].
\n
The process could be regulated, 40–70 h for the steeping, 70–200 h for germination and 6–20 h for the kilning [3]. The German Democratic Republic (GDR) continuous malting needed a total of 73–105 h, producing 10,000 t/a. The water was reused for steeping with an addition of 0.2% caustic soda [3].
\n
Moving pile systems by Ostertag, Seeger and Lausmann were turning and moving the malt under spraying or aeration with tempered air. Today tower malting is a semi continuous malt production. The steps on the floors take 9–24 h [4]. The batches are synchronised to the lorry sizes which fetch the malt to the breweries, the production speed is in a weekly rhythm, the silos buffer the amount of the barley harvest, and the demand of malt is by the annual variability of beer production.
\n
\n
\n
4. Continuous brewhouse
\n
The important utilities consumed in the brewhouse are malt grist, hot water for mashing and lautering, steam and water as a cooling liquid. In a batch brewhouse, different batches are processed at the same time and consequently lead to crucial electricity, steam and water consumption peaks.
\n
Milling: usually milling is a continuous process, no matter if wet mill, roller or a hammer mill is used. The malt silo and the milling body are the buffers before and after the milling. Capacities can be reduced, if the mashing process is continuous and not needing the tons per brew in less than 20 min to assure equal treatment of the grains. The comminution degree is dependent on the lautering process following. If continuous the husks do not need to be maintained, like for lautering. This means more yield but less blank worts.
\n
Mashing: infusion mashing is easily to be performed continuously. Plate or tube heat exchangers vary temperature and speed of the mash. Plate heat exchangers have limited applications for products with particles and/or fibres, while tube heat exchangers have the lower energy recovery rates [5]. Mash cannot run against mash like in a plate heat exchanger. An intermedia water circuit is necessary to recuperate energy in the casing pipe. Mixing while heating has to assure equality of the treatment as much as possible.
\n
In 1998, Meura started the development of the continuous brewing concept. A complete pilot plant was installed in 1999. The first operation of the Meurabrew on an industrial scale of 200 hl/h wort (up to 20°P) took place in 2007. A similar order for a plant in Fuzhou, China, was obtained. The entire operation is managed by 45 people, with just 2 men per shift to run the brewing operation from raw material intake to filtered beer during the daytime [1].
\n
Different mash vessels are keeping mash at constant temperature with a specific holding time. A continuous flow passes these vessels. Three parallel filters assure a regular continuous filtration process [1].
\n
Lautering: after the mashing process, vacuum rotary filters [6] or decanters may be used to remove the insoluble parts from the wort (Figure 1).
\n
Figure 1.
The continuous step of lautering in a “Pablo Brewhouse” adaptation in 1968 [7].
\n
A process for the continuous production of wort was described by Harsanyi in 1968 [7]. It was substantially characterised by separating the mash continuously by centrifugal action in at least two stages, sparging the largely dehydrated solids fraction with controlled quantities of water removing the dehydrated solids automatically and subjecting the wort obtained to further clarification before delivering it to the brew kettle. The separation of the liquid mash fraction from the solids is accomplished by means of a special type of centrifuge. The centrifuge has a housing in which a conical, perforated drum rotates. The housing has a first chamber with two compartments and separate liquid cutlets and a second chamber for the removal of solids, the second chamber being arranged at the larger diameter drum end. The mash slurry to be separated is delivered into the centrifuge at its smaller diameter drum end. Disposed within the drum is a hollow shaft having two separate liquid passages to which jet pipes are connected. The shaft rotates at a speed slightly less than that of the perforated drum [7].
\n
Similarly, the continuous lautering system Nessie from Ziemann works, introduced to the public in 2016 [8]. The separation of the mash is carried out via four filter units in cascade arrangement, in which the rotary disc filters perform the separation of wort and spent grains. The sparging of the extract is carried out in parallel using a turbulent counterflow extraction [8]. The time saved is about 160 min (34%) per brew [9]. Worts are less blank and contain more fatty acids, more zinc and less polyphenols. This increases the fermentation speed and the flavour stability [10]. Continuously produced worts have different qualities compared to batchwise-produced worts [3].
\n
Wort boiling: the wort boiling process suits to obtain the following objectives [11]:
Extraction and isomerisation of hop components
Hot break formation mainly by coagulation of proteins
Formation of colourant substances
Formation of reducing aromatic compounds by Maillard reaction
Evaporation of undesired volatile aroma compounds
Decrease of the pH of the wort
Inspissation of the wort
Sterilisation of the wort
Inactivation of the malt enzyme fixation of the wort composition.
\n
This can be done continuous and faster at higher temperatures. Continuous high-temperature wort boiling (C) or high-temperature boiling (HTWB) is an alternative boiling system. The idea is quite old as Dummet described such a system in 1958 and Daris et al. in 1962 [20]. At high temperatures of 130 or 140°C, very satisfying wort analysis data could be obtained although very short boiling times of ca. 5 min were used. The wort was heated up in three steps. In the first two steps, vapour from the flash-off chambers was reused. Considerable energy savings could be obtained due to the short boiling time and energy recuperation. Alternative continuous systems have been developed [11]. A boiling aroma was a limiting factor to the temperature. Most breweries using high-temperature boiling were closed or had to reduce the boiling temperature, as some boiling tastes were not beer typical. Reducing the temperature declined the degree of energy recuperation from the vapours.
\n
Chantrell describes the process of a practical 300 hl HTWB in Great Britain, where HTW was popular in the end of the last century [12]. Wort heating and boiling are influenced by the degree of fouling, which is normal for a three-phase system with solid trub, liquid wort and steam [13]. This leads to flooring especially on the heating zone’s surfaces and to differences in the quality of the product in a series of batches. Wort quality changes over a series of brews without intermediate cleaning. The heat transport is decreased, and burnt aromas and trub affect the wort. The thermal load on the wort has to be increased because of the heating profile, which has to be adapted in order to compensate the decreasing heat transfer rate caused by the fouling layer [12, 23].
\n
The wort is collected in the existing kettle at approximately 72°C from the lauter tun. Hop addition and adjustments to colour and gravity are made at this stage. The wort is then pumped through the HTWB to the whirlpool separator. Inside heat exchangers the wort temperature is raised in three successive steps to 140°C. At this temperature, the wort is held for 3 min while passing through an insulated holding tube. The wort then passes into an expansion vessel where the pressure is reduced to a predetermined level. In a second expansion vessel, the pressure is reduced to atmospheric pressure. Energy recovery is achieved with the flash vapours of these two expansion stages. They are used to heat up the first two wort heat exchangers. Only the third heat exchanger requires an external steam supply for the trim heating of the highest temperature. Cleaning of the plant is performed by automatic control and involves three cycles: firstly, the weekly cleaning of the two expansion vessels via spray balls and, secondly, the cleaning of the vapour side of the first and second stage heat exchangers. This cycle is only operated every 2–3 weeks depending on throughput. The main cleaning cycle which follows the wort path through the plant is operated at the end of the 5-day brewing week. Initially, cleaning problems were encountered with the large-diameter holding tubes. These large tubes were difficult to fill, and the low detergent velocity provided no scrubbing action against the protein deposits. The problem has been overcome by the dosing of hydrogen peroxide into these tubes during the caustic cycle. Foaming agents ensure complete cleaning of this section of the plant. The dosage rate for the peroxide is 0.1% by volume of a 30% hydrogen peroxide. For the third stage the heat exchanger is equipped with an automatic self cleaning cycle, using steam to crack the layers usually after one or two thousand hectolitre [12].
\n
At the Meura brewhouse, continuous wort boiling was combined with a hop strainer, if natural hops were used [1]. Decanters or centrifuges were necessary to remove hot break and to avoid yeast slime, especially if continuous fermentation is following. The wort was heated up in-line to boiling temperature. The added hops were homogenised. An adapted agitator assured a sufficient mixing for the trub formation. For the chemical/biochemical reaction of turning the S-methylmethionine into dimethyl sulphide, an external agitation must be provided. Clarification is necessarily conducted prior to stripping to avoid fouling the column with hot trub. The wort-settling tank is needed to recover non-oxidised trub from the hot wort in a continuous way. From the clarification unit, the wort is then stripped by a single pass stripping column. The unwanted volatile components are stripped by counterflow clean steam. The wort is pumped continuously from the bottom of the stripping column through the wort coolers. Because fouling is unavoidable, two duplicate built wort coolers have to assure a continuous cooling of the wort; one wort cooler can be cleaned, while the other one is cooling the wort.
\n
Wort cooling can be done within 50–60 min generating a peak consumption during this period. Compared with batch brewhouses, the heat losses and peak of utilities are lower during continuous processing. While batches are pumped from vessel to vessel, air enters the vessels, pipes and valves at each transfer, thus cooling down the facilities. The transfer of batches also enhances the extract, water and energy losses since vessels are never emptied completely [1]. Water evaporates, and sugars, polyphenols and proteins concentrate, forming layers that have to be cleaned before biofilms come up. Continuous systems can be kept in a stable equilibrium for a longer time and need less cleaning, if kept in a hygienic status. This is even more important in the cold section of a brewery.
\n
\n
\n
5. Continuous fermentation
\n
Continuous wastewater treatments with aerobic and anaerobic microorganisms are big continuous fermentations and show the sensitivity of balancing the biological process. Continuous beer fermentation has to fulfil not only the metabolism of substrate. A system of continuous beer fermentation was patented in 1906 by Van Rijn [2]. In 1953 Morton Coutts patented a process known as continuous fermentation at the Waitemata Brewery in New Zealand, which eventually become DB Breweries [14].
\n
Ricketts (1971) referred to continuous beer fermentation systems which date from the end of the nineteenth century. During the 1960s, while introducing large uni-tanks, interest arose in permanent fermentations. Several systems were developed, and some reached the point of marketability. Some of the anticipated benefits of continuous fermentation were realised, but most breweries, with some notable exceptions, have continued to use the traditional batch approach, using large-capacity vessels [2].
\n
Coutts created a “wort stabilisation process” that clarified the wort and made it more consistent. He separated the main functions of the yeast into two stages, first the yeast growth and then the fermentation. By splitting these two functions, Coutts created a “continuous flow”. The brewers had to add raw materials continually to the first stage and draw off a steady gain of finished beer from the second stage, thus allowing the process to run constantly [14].
\n
The rate at which wort is produced must be sufficient to supply the needs of the yeast at all the time. Inevitably, this requires a prefermenter wort collection vessel. Downstream of the fermenter the brewery has to be capable of handling a continuous supply of green beer. Consequences of failure in a continuous fermenting system cause a serious threat to production. Emptying, cleaning, starting a new process and establishment of stable running conditions are long procedures that take at least 2 weeks in time [2].
\n
The reactors took the form of a coil or similar elongated form by using multiple tanks with a continuous wort flow. The open continuous culture system may also consist of a stirred reactor to which medium is introduced by an entry pipe [2]. The rate of medium addition can be altered by a frequency-controlled speed pump, which is controlled by sensors checking the state of fermentation by pH, density, turbidity or gas production. Culture yeast is removed from the reactor via a second pipe which is arranged in the form of a syphon.
\n
In 2013 Müller-Auffermann at the Technische Universität München installed a downward-facing pipe with two reaction zones in each tank. Four tanks could be filled and emptied continuously from the top part of the tanks. The tanks were combined to a reaction cascade. They were equipped inside with a central pipe, with open bottom. The bottom connection of the tank could hence be used to discharge yeast cells and other particles during the process [15]. Inoculation and growth medium were mixed at the point of entry and fed simultaneously and continuously into the reactor. Within a discrete “plug” is travelling through the reactor. A minimum of backward and forward mixing had to be assured. So batch growth proceeded. The reactor could be viewed as a continuum of batch cultures. The spatial location is related to culture age. The factors of temperature, inoculation rate and substrate concentration are also influential in a plug flow continuous culture, like in a batch culture. The composition of the culture issued from the reactor is a function of the flowrate. By careful regulation of these parameters, it is possible to establish a steady state at which the product is of a constant and desired composition [2]. Biomass recycling will be a further refinement to be introduced to a plug flow reactor. The biomass is returned to the entry point of the reactor where it is used as inoculum. Used in this way, the reactor requires only to be supplied with fresh substrate [2].
\n
The prolonged nature of continuous fermentation has inherent risks. Extended running times increase the opportunities for microbial contamination. Yeast “variants” may be selected with the concomitant risk of undesirable changes in beer quality. Continuous systems are more sophisticated than many brewery batch fermenters. Skilled personnel must be on-site, night and day, to provide technical support, if a deviation is indicated [2].
\n
In comparison to classical batch fermentation, only one or few fermenting vessels are needed. Furthermore, beer losses are reduced, less pitching yeast is needed, and detergents and sterilants are saved. As long as the process is stable, a consistent beer quality can be expected. Microbiological contaminations or yeast mutation leads to serious consequences [2] especially if no second production line or some beer for blending is available to keep up the delivery capacity.
\n
Continuous fermentation systems, based on immobilised cells, were condemned to failure for several reasons. Engineering problems like excess biomass, problems with CO2 removal, optimisation of operating conditions, clogging and channelling of the reactor, unbalanced beer flavour, altered cell physiology and cell ageing lead to unrealised cost disadvantages such as high carrier prices at complex and unstable operations [16]. Pilot-plant and full industrial-scale processes showed engineering problems. The carrier material, the reactor design, together with the effect of immobilisation on yeast physiology, and the risk of contamination end up in a hardly predictable flavour profile of the beer produced. Therefore, despite the economic advantages expected, the continuous process has so far been industrially applied only in beer maturation and alcohol-free beer production [16].
\n
The crucial step forward in continuous technology was certainly the development of commercial immobilised yeast reactors. This approach was of sufficient interest to form the subject of an entire European Brewing Convention Symposium “Immobilised Yeast Applications in the Brewing Industry” held in Finland in 1985 [17, 18]. The advantage of immobilised reactors is that very high yeast concentrations are achievable. This allows a very rapid process throughput which is of particular benefit when applied to rapid beer maturation. A single immobilised yeast reactor can eliminate the time-consuming warm conditioning step for diacetyl reduction at the end of a lager beer fermentation [2].
\n
The application of gel occlusion systems in the brewing process, even if associated with many advantages over conventional fermentation technology, has some important drawbacks, particularly diffusional limitations which impact negatively on yeast growth, metabolic activity and beer flavour, Masschelein et al. concluded at EBC Congress in 1984 [18]. Nakanishi et al. recognised that fermentation activity in continuous working fermenters fell gradually during continuous operation of the system. It could be maintained for 2 months by periodic aeration in which 290 mg/g-yeast (dry matter) of oxygen was supplied to the immobilised yeast [17].
\n
Continuous fermentation suits best in breweries making only one style of beer, because its time and capacity consume to stop the process and start up again with a new beer [14]. Immobilised yeast reactors have also found use in new fermentation processes, for example, in the production of low-alcohol or alcohol-free beers [2], where yeast has more clarification tasks than fermenting and propagation. The major strength of the batch system, using several vessels, is that it is able to cope with seasonal or shorter-term fluctuations in demand. It can easily be adapted to vary the spectrum of production of several different beer varieties and qualities. On the other hand, benefits of continuous fermentation are realised when the systems are operating at a stable status for a long period of time with minimum downtime for changes in beer quality [2].
\n
\n
\n
6. Maturation
\n
Some of these yeast metabolism byproducts (vicinal diketones, acetaldehyde, dimethyl sulphide) impart undesirable flavours to the green beer. The main aim of maturation is to reduce the concentration of such unfavourable flavour compounds in the green beer, to saturate the final beer with CO2 and to remove the haze-forming components from beer within 7–30 days [16]. Fumigation with CO2 under counter-pressure to avoid too much foam may strip the unwanted flavour. The flavour can be removed from the CO2 with active carbon so that the CO2 may be collected, compressed and used for further tasks. Continuous clarification is best done with centrifuges or decanters.
\n
\n
\n
7. Filtration
\n
Filtration and stabilisation of the beer are carried out in order to achieve microbial, colloidal and flavour stability so that no visible changes occur for a long time and the beer looks and tastes the same as when it was made [16]. Particular for higher amounts of yeast cells, tangential flow or crossflow filters are good prefilters before flash pasteurisation or membrane filtration. Although batch flushes can extend the continuous filtration of a crossflow filter, fouling layers will clog the membranes. A chemical recovery of the filter modules is necessary; the continuous process has an end. Usually bright beer tanks collect a batch for the final quality control, and they are the buffers for the following filling of the beer in bottles, cans, kegs or road tankers.
\n
\n
\n
8. Continuous bottling
\n
The most expensive and labour-intensive part of the value creation in breweries is the bottling part. Here most breweries produce continuously, as several machines are needed. Depalletisers for new or crated return bottles, washing machines or rinsers, inspection machines fillers, pasteurisers, labelling machines, packers, wrappers, shrink machines and palettisers run more or less continuously. Stops and interruptions have to be buffered by the conveyers, usually able to keep machines running, while the other needs time to repair so that the previous or following machines need not stop. Modern bottling lines have frequency-controlled conveyers and machinery so that the assembly, connected by system bus, can alter their speed to keep up the continuous production.
\n
The big challenge is changing of the products, the beer type, the labels or the shape and size of the bottles. In bottling, when the beer arrives filtered, sterile and stable, mostly physical deviations have to be handled. Product safety has to assure clean, not contaminated bottles. Camera systems or even gamma or X-ray is used to check the bottles, cans or kegs. Rejected containers have to be replaced by the following shipshape containers. Bottle burst leads to splinter showers, where open bottles have to be removed, eventually contaminated by sharp-edged glass.
\n
Mistakes in this process certainly propagate downstream, if not corrected immediately. A dirty bottle becomes a dirty filled and corked bottle, is labelled and packed and—in the worst case—is sold and consumed. Sensors and camera systems should check the system at the highest accuracy possible, as the process goes on and might lead to big amounts of unsafe products at the far end of the beer production and the intersection to the customers and consumers. The more precise the process is performed, the safer the product and the more the consumers’ expectations can be met.
\n
\n
\n
9. Inaccuracy of production, measuring, controlling and quality forecast
\n
One problem of continuous fluid dynamics is the dwell time in the system. Flow conditions ought to be simulated in flow models. These have to be simulated and calculated to predict rheological behaviour and chemical or biochemical reactions [13]. In production methods, biologically grown raw materials or process measurements have a certain inaccuracy or mistake, and results cannot be determined. They just can be estimated mathematically.
\n
The average remaining time in a system is not similar to the real remaining time in a system. Molecules or particles entering a system at the same time may have different remaining times [13]. If there are two or more phases, like fluid and solid or gas particles, the continuous phase (usually the fluid of the beer and the foam) may behave totally different from the solids and the gas phase (bubbles) [13]. Coalescence will be influenced by the collision frequency and the behaviour of the substances during the phases [13]. Biochemical processes have lots of influencing factors, like temperature, pH value, viscosity, surface tension, osmotic and hydrostatic pressure, concentration gradients, mechanical influences, electrical effects, zeta potential and many more. Especially microorganisms change their behaviour under different conditions. As evolution does not stop at the brewing vessels, genetic deviations may cause different behaviours of raw materials and microorganisms.
\n
If more than one species is present, synergetic or suppressing effects may end up in biofilms and uncontrollable developments. Working with just one species—mostly Saccharomyces—is quite predictable in its final products. As soon as more species come up, things get more and more unpredictable. Hygiene helps to keep processes under control. Dead zones may become lively areas, and biofilms have to be avoided. Hygienic difficulties in cleaning have to be respected, and in fact, continuous systems cannot be cleaned as often, as batch containers if used for a long time.
\n
This causes problems, especially if brands need to have a constant quality to fit to the consumers’ expectation which connected to the brand. Lots of homogenisation equipment during continuous production achieve a dense remaining time in the reactors. Energy, shear forces and moving parts are usually combined with abrasion of wear parts, which means a continuous change of quality of the machinery. Preventive maintenance needs to stop the processes and to start them again.
\n
\n
\n
10. Mathematical, physical, chemical and biological limits by error propagation in continuous production
\n
A lot of factors have an influence on each step of malting and brewing. This ends up in a broad range of quality factors. Especially biologically balanced equilibriums react to changing conditions by complicated, not predictable effects, which can hardly be measured or recognised by sensors (Table 3).
\n
\n
\n
\n
\n\n
\n
Gas phase
\n
Liquid phase (continuous)
\n
Solid phase
\n
\n\n\n
\n
Volume Pressure Temperature Gas equilibriums Bubble size Bubble form Molecular size Heat capacity
\n
Temperature Viscosity Surface tension Density Hydrophobic substances Hydrophilic substances pH Heat capacity
\n
Form Size Surface, roughness Density Zeta potential Hydrophobic liquids Hydrophilic liquids Heat capacity
\n
\n
\n
Altered/influenced by
\n
\n
\n
Temperature
\n
Temperature
\n
Temperature
\n
\n
\n
Pressure
\n
Shear forces
\n
Shear forces
\n
\n
\n
Microorganisms
\n
Microorganisms
\n
Microorganisms
\n
\n
\n
\n
Enzymes
\n
Enzymes
\n
\n
\n
Time
\n
Time
\n
Time
\n
\n\n
Table 3.
Influencing physical and microbiological factors during beer production.
\n
Each quality parameter and each sensor, used during the processes, have its specific standard deviation and impreciseness during measurement. This can be respected, when intermedia products are checked for their quality. Corrective arrangements can be used to reach the final quality aim. During continuous production error, propagation may lead to a huge deviation in quality, which is also caused by the given impreciseness of the sensors who should avoid this, especially if lots of sensors are used in following steps to automatically control the continuous production [21].
\n
An imprecise thermometer in the mash process will lead to different amounts of sugars or proteins, which can behave differently in the wort vessel than the wanted product. This may lead to different colours or yeast behaviours. The thermometers, used during fermentation, have a certain deviation as well, which might lead to different metabolisation products. They can be a favourable substance for other yeast or bacteria strains which also create unwanted flavour products. Sensors may detect but also have a deviation which allows unwanted processes. If sensors show a deviation to the quality aims, a continuous system should be able to adjust the process to the predetermined values. If this is not possible, the process has to be stopped. Analysis of the intermedia product may help to decide how the quality targets can be reached by the following process step, in the way batch processes are successfully managed.
\n
The deviations in a naturally given physical, chemical and biological concentrations sum up and propagate by each sensor deviation and error used in the process. Calibration might help to a certain extent, but the amount of sensors is growing in continuous processes, so the amount of possible imprecise information is steadily increasing with the amount of measurements and control valves and regulations, which also have a certain deviation. Measurable substances like diacetyl, gravity, conductivity and turbidity are useful, but they are just single parameters in a bunch of varying aroma particles, dependent on microbiological, chemical and physical balances.
\n
In statistics, “propagation of uncertainty” also called “propagation of error” is the effect of variable uncertainties combined. Errors, or more specifically random errors, result in an uncertainty that builds up during long-lasting consecutive processes. Measured values are necessary to control the process. All measurements have uncertainties due to limitations (Figure 2). Instrument precision and other deviations propagate due to the combination of variables in the function [19].
\n
Figure 2.
Mathematical estimation: example for additive error propagation [22].
\n
If biological changes occur in raw materials or microorganisms, errors do not add but multiply, or exponentiate, as several factors change: aromas, natural substances, pH, viscosity, chemical balances, concentrations, compositions, sublimation, dissolving, evaporating, etc. Respecting the rheologically unpredictable behaviour of altering three phases during rheological processes [13], a constant and planned quality can only be expected for a certain time; quality changes and emptying and cleaning of the system become necessary.
\n
Continuously changing products like mash, fermenting of green beer has to come to a stable and defined quality, which fits to the consumers’ expectation. This is traditionally achieved by cold stabilisation during maturation, which is also a possibility to check and adjust quality, to blend the beers, and it is a buffer before bottling and racking. Filtration and pasteurisation help to stop the most biological and enzymatic processes. Especially before bottling a quality check has to be conducted, to avoid faulty products being bottled. The product will be kept during distribution and at the consumer’s place for a certain time, where it should not change anymore. Finally the consumer’s expectation cannot be adjusted to an unpredictable quality (Figure 3).
\n
Figure 3.
Deviations in batch and continuous brewing processes.
\n
\n
\n
11. Conclusion
\n
Stepwise batch production has been added by continuous brewing processes for more than 100 years. Calculations may predict the results in biological processes to a more accurate degree, if computers obtain more and more data to use. The results in the end are estimations, relying on the number of factors in relation to their influencing parameters. The possibility to measure these factors need to have a known accuracy. This will stay the biggest challenge, as long as natural ingredients and microorganisms play a main role in malting and brewing. Fermenting with more than one yeast strain quality, prediction falls back to the times of spontaneous fermentation, where only few products were of a good taste by accident. The same thing occurs, if contaminations get into the continuous systems, when cleaning always implies a long interruption of the process, especially a long time to adjust the following process up to a stable equilibrium.
\n
Many substances in beer are known, many not. Some substances or physical properties are useful to define the quality of the beer and the intermedia production steps. Few parameters can be used to control the brewing process in the direction of a defined quality aim. All of the raw materials, the determination methods, the regulation equipment and the biological reactions of the microorganisms have deviations, some of them are not measurable or known yet. In a batch process interstage products like malt, wort or fermented beer can be checked, and the following processes can be used to adjust the quality goals. Reactions to the deviations in continuous processes should set the system back to the target conditions. Combining several steps to a continuous process with continuous adjustment can be controlled by a self-learning by fuzzy logic or artificial intelligence. As these computers need data from precise sensors to control precise valves, stirrers, pumps, etc., this will lead to a predominantly maintaining, calibrating and scrutinising reaction to the system instead of brewing and creating the quality of the beer.
\n
Nowadays a continuous step is followed by a batch step. The possibility in adjusting the quality with following processes should be given. Also blending needs buffer capacities to equalise the beer to consumer’s expectation. For offering different beer types, also blending beverages are necessary to be added. The continuously produced beer might supply a base beer, which is blended before filtration with other batch process beers or water, aromas and lemonades in a nontraditional way.
\n
The brewing process can be performed in steps, continuously and automatically. Perhaps one day continuous processes will be longer stable. The processes may also expire in constant quality results. But brewing is fun, fun that should not be left to the machines.
\n
\n\n',keywords:"continuous process, malting, brewing, fermentation, filtration, bottling, error progress, multisensor behaviour",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/67821.pdf",chapterXML:"https://mts.intechopen.com/source/xml/67821.xml",downloadPdfUrl:"/chapter/pdf-download/67821",previewPdfUrl:"/chapter/pdf-preview/67821",totalDownloads:1243,totalViews:0,totalCrossrefCites:0,totalDimensionsCites:1,totalAltmetricsMentions:1,impactScore:0,impactScorePercentile:36,impactScoreQuartile:2,hasAltmetrics:1,dateSubmitted:"March 8th 2019",dateReviewed:"May 18th 2019",datePrePublished:"June 24th 2019",datePublished:"February 5th 2020",dateFinished:"June 24th 2019",readingETA:"0",abstract:"Although the barley and hop harvest is a batch process, the ingredients are storable to a certain extent, so malting and brewing can be performed continuously. The more expensive machinery and energy are becoming, the more continuous production is becoming efficient. The advantages are smaller capacities, less energy consumption and more recuperation. Most filling processes run semi continuously, and energy-consuming processes like malting can also run continuously around the clock. Disadvantages are necessary buffers, microbiological contamination and less flexibility in producing different types of beer or adjusting the production to seasonal fluctuations. Mistakes and errors increase, even if accuracy and in-line sensors help to keep quality stable for some time. This article discusses benefits and limits of continuous technology.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/67821",risUrl:"/chapter/ris/67821",book:{id:"8244",slug:"new-advances-on-fermentation-processes"},signatures:"Mark Strobl",authors:[{id:"298220",title:"Dr.",name:"Mark",middleName:null,surname:"Strobl",fullName:"Mark Strobl",slug:"mark-strobl",email:"mark.strobl@hs-gm.de",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. History of continuous beer production",level:"1"},{id:"sec_2",title:"2. Pros and cons of continuous production versus batch process",level:"1"},{id:"sec_3",title:"3. Continuous malting",level:"1"},{id:"sec_4",title:"4. Continuous brewhouse",level:"1"},{id:"sec_5",title:"5. Continuous fermentation",level:"1"},{id:"sec_6",title:"6. Maturation",level:"1"},{id:"sec_7",title:"7. Filtration",level:"1"},{id:"sec_8",title:"8. Continuous bottling",level:"1"},{id:"sec_9",title:"9. Inaccuracy of production, measuring, controlling and quality forecast",level:"1"},{id:"sec_10",title:"10. Mathematical, physical, chemical and biological limits by error propagation in continuous production",level:"1"},{id:"sec_11",title:"11. Conclusion",level:"1"}],chapterReferences:[{id:"B1",body:'Bonacchelli B&HF. The Meurabrew the Brewhouse of the Future! [Online]. Available from: http://www.meura.com/uploads/pdf/Meurabrew%20Paper.pdf [Accessed: March 18, 2019]\n'},{id:"B2",body:'Buffalo_Brewing_Blog. Buffalo Brewing Blog » Brewery Convention [Online]. 2018. Available from: https://www.buffalobrewingstl.com/brewery-convention/continuous-fermentation.html [Accessed: March 17, 2019]\n'},{id:"B3",body:'Kunze W. In: Lebensmittelindustrie MfBIu, editor. Technologie Brauer und Mälzer. 5th ed. Leipzig: VEM Fachbuchverlag Leipzig; 1979\n'},{id:"B4",body:'Schuster WN. Die Bierbrauerei Erster Band Die Technologie der Malzbereitung. Stuttgart: Ferdinand Enke Verlag; 1976\n'},{id:"B5",body:'Krones. Krones.com. [Online]. 2019. Available from: https://www.krones.com/media/downloads/waermetauscher_en.pdf [Accessed: April 4, 2019]\n'},{id:"B6",body:'Narziß L. Back W. Die Bierbrauerei. Band 2: Die Technologie der Würzebereitung, 8th ed. Weinheim, USA: Wiley-VCH Verlag GmbH & Co. KGaA, John Wiley & Sons Inc; 2009\n'},{id:"B7",body:'Harsanyi DE. Kontinuierliche Schnellabläuterung nach dem Pablo-System. Brauwelt International. 1968;108(Nr. 45/46, 5./7):843-845\n'},{id:"B8",body:'Nessie Ziemann Holvrieka. Journal Beer [Online]. 2016. Available from: https://journal.beer/2016/11/14/nessie-revolutionizes-the-brewhouse-novel-mash-filtration-process-by-ziemann-holvrieka/ [Accessed: March 20, 2019]\n'},{id:"B9",body:'Klaus Wasmuht DE. Experience is the most Challenging Test. Brewing and Beverage Industry International. In: Sachon VW, editor. Mindelheim: Schloss Mindelburg; 2019\n'},{id:"B10",body:'Wasmuht K, Schwill-Miedaner A. With Omnium to Optimum. Rust, Baden: VLB Berlin; 2019\n'},{id:"B11",body:'Willaert RG, Baron GV. Wort boiling today—Boiling systems with low thermal stress in combination with volatile stripping. Cerevisia. Belgian Journal of Brewing and Biotechnology. 2001;26:217-230\n'},{id:"B12",body:'Chantrell NS. Practical experiences in the production of wort by continuous high temperature wort boiling. MBAA Technical Quarterly. 1984;21:166-170\n'},{id:"B13",body:'Kraume M. Verfahrenstechnik. 2nd ed. Berlin, Heidelberg: Springer Vieweg; 2012\n'},{id:"B14",body:'Brooks JR. Continuous Fermentation—Volume 29, Issue 4 [Online]. 2008. Available from: http://allaboutbeer.com/article/continuous-fermentation/ [Accessed: March 17, 2019]\n'},{id:"B15",body:'Auffermann-Müller K. More Sustainable Way to Brew Beer: Non-Stop Fermentation Saves Resources May 28, 2013, Technical University Munich. Read more at: https://phys.org/news/2013-05-sustainable-brew-beer-non-stop-fermentation.html#jCp [Online]. 2013. Available from: https://phys.org/news/2013-05-sustainable-brew-beer-non-stop-fermentation.html [Accessed: March 18, 2019]\n'},{id:"B16",body:'Brányik T, Vicente AA, Dostálek P, Teixeira JA. Continuous beer fermentation using immobilized yeast cell bioreactor systems. Biotechnology Progress. New York: American Institute of Chemical Engineers (AIChE); 2005;21(3):10\n'},{id:"B17",body:'Nakanishi K, Onaka TIT, Kubo S. A new immobilized yeast reactor system for rapid production of beer. In: European Brewery Convention Proceedings of the 20th Congress Helsinki, 1985. Helsinki: European Brewery Convention; 1985. pp. 331-338\n'},{id:"B18",body:'Masschelein CA, Carlier A, Ramos-Jeunehomme C. The effect of immobilization on yeast physiology and beer quality in a continuous and discontinuous system. In: Convention EB, editor. European Brewery Convention Proceedings of the 20th Congress Helsinki, 1985. European Brewery Convention; 1985. pp. 339-346\n'},{id:"B19",body:'Edu H. Physical Sciences 2 Harvard University, Fall 2007 [Online]. 2007. Available from: http://ipl.physics.harvard.edu/wp-uploads/2013/03/PS3_Error_Propagation_sp13.pdf [Accessed: April 7, 2019]\n'},{id:"B20",body:'Daris AD, Pllock JRA, Gough PE. Journal of Institute of Brewing. Weinheim, USA: John Wiley & Sons Inc.; 1962;68:309\n'},{id:"B21",body:'Belabbas B. DLR Institute of Communications and Navigation Multi-Sensor Integrity [Online]. Available from: https://www.dlr.de/kn/en/desktopdefault.aspx/tabid-7570/12813_read-32121/gallery-1/216_read-2/\n\n'},{id:"B22",body:'Gehlot T. Slide Share Error Analysis Statistics [Online]. 2014. Available from: https://www.slideshare.net/tarungehlot1/error-analysis-statistics [Accessed: March 22, 2019]\n'},{id:"B23",body:'Voigt DJ, Wasmuht K. Fouling during wort boiling—Effects on wort quality. Master Brewers Association of the Americas MBAA TQ. 2006;43:207-210\n'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Mark Strobl",address:"mark.strobl@hs-gm.de",affiliation:'
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1. Introduction
With the rapid development of human civilization, the consequent air pollution and greenhouse gas (GHG) emissions have threaten human being for years. Energy conservation and emission reduction is an increasing priority in the development of transportation industry.
A number of approaches can be used to improve energy efficiency and reduce CO2 emission, such as reducing aerodynamic resistance, transmission loss, tire rolling resistance, and weight [1]. Among these options, lightweight structure is currently considered as one of the most efficient solutions. In recent years, lightweighting has become a major research theme in the transport industry around the world.
Material substitution appears a promising option for lightweighting. Traditionally, steel, such as low-carbon steel and stainless steel, is used as main material to build major structure of transportation equipment. Titanium, aluminum and magnesium are promising lightweight metallic materials as alternatives to steel and cast iron. Among these materials, aluminum shows a balanced performance, such as light weight, good corrosion resistance, good formability, high specific strength and relatively low cost. Density of aluminum alloy is only one third of steel. Considering structural optimization due to material replacement, the overall weight of rail car-body is decreased by 50% when aluminum is used. This degree of reduction deserves an effort in engineering application.
Although aluminum accounts for ~8 wt% (by mass) of the earth’s crust, the high affinity of aluminum for oxygen, as well as the stability of aluminum oxides and silicates hindered its separation for a long time. For this reason, aluminum became economic for engineering applications only at the end of the nineteenth century [2].
Aluminum alloys was originally used in aviation industry which is an important part of the transportation industry. It was firstly used on Junker F13 fuselage with the invention of 2017-T4 alloy in 1920. It began to be used in the manufacture of train with increase of the train speed until 1980s. So far, it has been widely used in passenger cars with speeds above 200km/h, such as German ICE series high-speed EMU car-body, French ALSTOM double-decker TGV high-speed EMU car-body, Italy Pendolino(ETR) series pendulum high-speed EMU car-body, Japanese Shinkansen, and Chinese CRH high-speed EMU car-body and so on. Especially with the huge expansion of high-speed rail lines in China in the last 20 years, the development and application of aluminum alloy prospered.
A series of new manufacturing processes, such as friction stir welding and laser welding, were developed and applied. These technical progress promoted application of aluminum alloy not only in high speed trains but also urban rail vehicles, which continuously contributes to energy conservation and climate change. This chapter will introduce the application status and key technologies of aluminum alloy for manufacturing high speed train. It is expected that the technical analysis and summary will inspire and encourage material scientist and engineers to vigorously push technological innovation for comfortable travel experience in an environmental-friendly mode.
2. Characteristics of aluminum alloy
The special operating environment of trains and unique properties of aluminum alloy promotes the their combination, which favored the popularization of high speed train and in-depth application of aluminum alloy in transportation industry. For engineering application on rail transit vehicles, aluminum has the following advantages:
The net weight of the vehicle can be greatly reduced. The use of aluminum alloy can greatly reduce the net weight of rail passenger car while it meets the safety requirements in the aspect of strength and rigidity. Generally speaking, car-body made of aluminum alloy is 30%~50% lighter than that of steel. For high-speed and double-deck trains, the most effective way to make vehicles light is to increase the proportion of aluminum used in vehicles as much as possible.
Aluminum alloy has excellent fire resistance. Although the melting point of aluminum (660 °C) is much lower than that of steel (1530 °C), the fire-resistant of the car body is not only related to the melting point of the material, but also to the thermal conductivity of the material. Compared with steel, aluminum alloy has excellent thermal conductivity and better heat dissipation.
Aluminum alloy has good corrosion resistance. The surface of aluminum alloy is easy to form a layer of dense oxide film, which has a good anti-oxidation ability in the atmosphere. Therefore, car-body made of aluminum alloy has better corrosion resistance than that of steel, especially in the components that are not easy to be coated, such as the box structure and some of its internal beams and columns, aluminum counterpart show obvious advantages. At the same time, aluminum alloy surface can be colored, painted, sprayed, through chemical methods to greatly improve the corrosion resistance of the components, together with improved decorative effect.
Aluminum alloy is easy to process, manufacture and maintain. With the development and application of large hollow and complex section aluminum profiles, aluminum welding technology is constantly improving, and vehicle manufacturing technology is becoming more and more mature. Aluminum alloy parts is easy to be replaced, and suitable for all kinds of surface treatment. The workload need for manufacturing rail car-body is also greatly reduced than the steel one.
The price of aluminum alloy is moderate. The high price of aluminum material increases the manufacturing cost of vehicles, but aluminum alloy also makes vehicles light, which leads to the increase of transport capacity, reduction of energy consumption and reduction of maintenance costs. Taking the comparison of maintenance hours of vehicles leaving the factory at 10 A as an example, steel car is 100%, aluminum car is 52%.The recovery value of scrapped vehicles is 100% for steel vehicles and 480% for aluminum vehicles. From the perspective of comprehensive economic benefits, the use of aluminum vehicles is economical and reasonable. Therefore, the final cost of using aluminum alloy is moderate.
3. Typical aluminum alloys and their properties
Aluminum alloys commonly used in high-speed trains include 5000 series, 6000 series and 7000 series. 5000 series alloys show maximum strength and high corrosion resistance among the typical non-heat treatable alloy, which adapt to welded structure. 6000 series alloys show moderate strength and good corrosion resistance, together with perfect extrusion formability making complex and thin-wall hollow section possible. 7000 series alloys show excellent strength among age-hardening aluminum alloy, which provide wide space for weight reduction. Typical aluminum alloys for high speed train car-body are shown in Table 1.
Series
Designation
State
Mechanical properties
Application area
Note
Tensile strength/MPa
Yield strength/MPa
Elongation/%
5000
5083
O
≥275 ~ 350
125 ~ 200
≥16
Front skin
Plate
6000
6005A
T6
≥270
≥225
≥8
Underframe, sidewall, roof, end wall, beam
Plate, 3 ~ 5 mm
6005A
T6
≥255
≥215
≥6
Underframe, sidewall, roof, end wall, beam
Section, 3 ~ 5 mm
6082
T6
≥310
≥260
≥10
Underframe, end beam
Plate, 3 ~ 6 mm
6106
≥250
≥200
≥8
End wall
Section
7000
7B05
T4
≥315
≥195
≥11
Underframe, end beam
P, 2.9 ~ 6.5
7B05
T5
≥325
≥245
≥10
Underframe, end beam, traction beam
Section
Table 1.
Typical aluminum alloys for high speed train car-body [3, 4].
4. Detailed application
In railway vehicles, aluminum alloy is primarily used in manufacturing car-body and its ancillary structure. The car-body is a prolonged hexahedral structure. Aluminum is used to manufacture all parts of the structure, including roof, underframe, end wall and side wall. However, different aluminum designations are selected for different parts of the car-body based on their properties shown in section 3. Both extruded section and plate are used. Extruded profiles account for about 70% of the total weight of the aluminum alloy car-body, while the plates account for about 27%, and the castings and forgings account for about 3%. On the other hand, there is an emerging trend that aluminum alloy come into application to other structures such as gear-box and axle box than car-body. The detailed introduction is as follows.
4.1 Car-body
The development of aluminum alloy materials and large extruded profiles paves the way for the modernization and lightening of railway vehicles, In recent years, with the popularity of lightweight design concept for railway vehicles, as well as the requirement of simplified construction and maintenance, large integral thin plate and hollow complex thin wall profiles has been developed successfully. In Japan, 6N01(6005 alloy) alloy with better extrusion, welding and corrosion properties has been developed to produce porous complex thin-wall hollow profiles, widely replacing 7N01 and 7003 profiles as the floor, side plate and roof structure of the car-body. In Western Europe, aluminum alloy body is mainly made of 6005A extruded profiles, the main reason of which is that the extrusion performance of 6005A is better, the production process is more simplified, and the stress corrosion problem of 7000 series alloy can be avoided. The application of typical aluminum alloys on 300 series Shinkansen high speed train is shown in Figure 1. A complete car-body is shown in Figure 2. As is shown, the car-body is mainly composed of extruded section.
Figure 1.
Application of aluminum alloys on car-body of Shinkansen 300 series high speed train.
Figure 2.
Typical car-body of high speed train made of aluminum alloy.
The car-body can be easily welded automatically with through - length welds, as shown in Figure 3. The aluminum section profiles can be designed according to the section structure of the car-body, as shown in Figure 4. Typical extruded section profiles for high speed train are shown in Figure 5.
Figure 3.
Typical sidewall of high speed train composed of extruded sections.
Figure 4.
Part of a section structure of high speed train car-body.
Figure 5.
Typical extruded section profiles for high speed train.
However, when it comes to the head car, the structure is quite different. In order to achieve optimal aerodynamic performance running at high speed, a streamlined design was given to the head car, as shown in Figure 6. This unique shape make the it impossible to manufacture with relatively regular and straight sections. Therefore, beam and slab structure became the optimum option for head car. As shown in Figure 7, a framework is designed based on the requirement for stiffness and strength to support the front skin against plastic deformation. It is welded with hundreds of beam made of aluminum plates prior to skin fixation. Afterwards, the skin is divided into small pieces based on the principle of good workability. Each piece is deformed to specific shape based on the design profile. Then the piece is fixed on the framework one after another, shown in Figure 8.
Figure 6.
Streamlined head car of CRH 380A high speed train.
Figure 7.
Internal structure of the head car of high speed train.
Figure 8.
Head car of high speed train.
4.2 Gear box
For further reduction of the weight of the train, it is obviously not enough by reducing the weight of the car-body because the car-body accounts for only about 20% of the total mass of the train. Key components of bogie including traction motor, wheelsets, frame and braking system attracted attention of proponents of lightweighting. The lightweighting of gear box can help to reduce unsprung mass and wear or damage to rail. In this part, the application of aluminum alloy on gear box is introduced.
Gear box of high speed train is manufactured by casting aluminum rather than wrought alloy due to complex and unequal thickness. Low pressure casting is widely used in non-ferrous alloy casting because of its high feeding pressure and temperature gradient and stable filling, which can effectively improve the density of castings and product yield.
AlSi7MgA and AlCu4Ti are commonly used as casting materials for gear box due to good flowability, low thermal expansivity and shrinking percentage. Typical aluminum gear boxes on CRH series high speed train are shown in Figure 9.
Figure 9.
Typical aluminum gear boxes on CRH series high speed train.
4.3 Axle box
Axle box is one of the important bearing parts of trian bogie and transfer joint of motion. The left axle box part is installed on the axle journal through a rolling bearing, and the right swivel arm is connected with the positioning swivel seat on the frame through an elastic node. When the train is running, axle box bears the action of vertical force, longitudinal force and transverse force.Therefore, the bearing condition of the axle box body is complex, and its structure and performance stability are very important for the safe operation of the train. 7050 aluminum alloy forgings shows high strength and toughness, which can significantly reduce unsprung weight. The weight of forged aluminum product decrease 62.5% as compared to the traditional carbon steel one. Therefore, forging aluminum alloy axle box is widely used on high-speed train. 3D model of an aluminum alloy axle box is shown in Figure 10. A typical finished aluminum alloy axle box of highspeed train is shown in Figure 11.
Figure 10.
3D model of an aluminum alloy axle box.
Figure 11.
Finished aluminum alloy axle box of high-speed train.
5. Key manufacturing techniques
The engineering application of aluminum alloy in the field of rail transportation encounter a series of challenges, which promote the development of a series of key manufacturing techniques including casting, forming welding and anti-corrosion processes.
5.1 Casting
Casting is an important process for the manufacture of complex structures such as gear box. The main challenges of gearbox casting process are as follows:
Ensure there is no cold partition, porosity and other defects in the thin-walled area, so as to meet the requirements of casting appearance quality.
Ensure there is no excessive shrinkage porosity, shrinkage cavity and other defects on the box surface, flange, hanging and other key parts, so as to meet the internal quality requirements of castings radiographic inspection.
Ensure a high requirements for machining surface pinhole and non-machining surface, especially for aluminum alloy castings that are easy to produce pinhole defects.
In the production of aluminum alloy gearbox for high-speed EMU, the common casting defects include porosity, pinhole and shrinkage cavity.
In order to eliminate those pores, two measures need to be taken on the premise of controlling the air production content of molding sand. Firstly, the venting near the inner runner and riser should be improved by opening more air hole and hollowing out the loam core of the outer molding. Secondly, thickness of the coating should be guaranteed to decrease surface void on the sand (core) by using coating with high thermal conductivity such as zircon powder. The filling pressure and holding pressure are increased appropriately, so as to increase the resistance of gas entering the metal liquid.
The key of eliminating pinhole mainly relies on the control of hydrogen content in liquid aluminum. The refining process can reduce the oxidation inclusion and hydrogen content in liquid aluminum, and thus effectively reduce pinhole forming tendency.
Regarding to shrinkage cavity at the top of gear box, it is proved effective by simulating the solidification process with MAGMAsoft. Chilling block and riser locating can be optimized to ensure the feeding channel of the top riser unblocked under reasonable temperature distribution.
5.2 Forming process
There are four different technologies available to manufacture the front skin panels of the head car. The most commonly used one is the hammer press where a hammer machine is used conveniently to produce the target shapes. However, the dimensional tolerance of the produced product heavily depends on the worker’s experience. After installing the panels on the structural frame, any further modifications of the geometrical features can only be completed by using the hand tools such as hammer. Such a manual process renders the high repeatability of manufactured components almost impossible. The second technique is the expanding-stretching process. It is applicable for the panels with curved profile but only to a certain extent. Additionally, the rotating press machine, which mainly aims for manufacturing a panel with small and uniform curvature, is used, while a process called mould press that uses mould to produce the target shape is employed for the panel with complicated and small curvature.
As shown in Figure 12, the front skin of a typical CRH 380A high speed train is divided into around 70 small pieces which are joined together through a total of 170 meters long welding line. As the length of each panel is limited to only 1 meter, the manufacturing process becomes time consuming and low efficient for producing a considerable amount of small components. The product quality of the front panel is also compromised due to the increased residual stress resulted from the uneconomical and complicated assembling process.
Figure 12.
Front panel of CRH 380A high speed train.
In order to ensure the assembly precision of each piece, a commercial finite element analysis simulating the skin drawing and springback process based on flexible multi-point die is necessary. In order to improve the computational efficiency and obtain satisfactory computational accuracy, the dynamic explicit algorithm is used to simulate the drawing process, and the static implicit algorithm is used to calculate the springback. Figure 13 shows the Distribution of MISES stress and equivalent plastic strain for forming of a single piece. The forming process is developed based on the simulation results which can save experimental time and improve adaptability to different products, shown in Figure 14.
Figure 13.
Distribution of MISES stress and equivalent plastic strain for forming of a single piece.
Figure 14.
Skin drawing based on flexible multi-point die.
5.3 Welding
Due to special thermal physical properties and welding characteristics of aluminum alloy, such as low melting point, thermal conductivity, high electrical conductivity and the expansion and shrinkage, high content of alloy elements as compared to carbon steel and stainless steel [5, 6], it is much more easily to produce pores, crack, lack of penetration, incomplete fusion, large welding deformation, bite edge and slag when aluminum alloys are welded. The assembly precision, quality and performance can be severely affected by welding process. Therefore, the welding process is quite crucial in the manufacture of high speed train.
The following aspects should be considered as the basis of selecting welding method:
Aluminum alloy is coated with a dense oxide film which can easily adsorb moisture and bring hydrogen to molten pool. The aluminum can also become oxidate slag existing in the weld which affecting the performance. Therefore, it is very important to remove the oxide on the base metal and groove surface of aluminum alloy before welding.
The thermal conductivity of aluminum alloy is five times that of low carbon steel. High power or energy concentrated welding heat source should be a preferential option. And preheating is necessary for thick plate welding.
The thermal expansion coefficient and cooling shrinkage rate of aluminum alloy are two times that of steel. Therefore, aluminum alloy melting welding deformation is serious. Deformation control measures such as reverse deformation and reinforcement constraint should be considered.
Aluminum alloy material has different kinds of alloying elements, and the loss of alloying elements during fusion welding is easy to lead to the decrease of joint strength and corrosion resistance, and the weld metal and heat affected zone are easy to produce intergranular cracks. Cracking susceptibility should be considered.
In this section, three typical welding processes which are widely used in manufacturing process of high speed train are introduced.
5.3.1 Arc welding MIG
Pulse MIG welding is the most widely used and developed method of aluminum alloy welding which is characterized by large thermal power, high linear energy, good energy concentration and good protection effect. The above features are suitable for welding aluminum alloy based on its unique thermal physical properties. Pulsed MIG welding can be used to control wire melting and droplet transition, improve arc stability and achieve droplet jet transition with small average current, thus suitable for all-position welding.
Considering the cathodic atomization effect of MIG welding on removing aluminum oxide film, DCEP (Direct Current Electrode Positive) is commonly used for pulse MIG welding. Semi-automatic pulse MIG welding are fit for irregular short welds while regular long straight welds are usually automatically MIG welded with laser tracking, shown in Figure 15.
Figure 15.
Automatically MIG welding process.
Typical defect includes poor formability, burning through, excessive penetration, cracking, pore and slag, as shown in Figure 16.
Figure 16.
Typical defect in aluminum alloy weld.
5.3.2 FSW welding
In addition to the traditional MIG welding, friction stir welding(FSW) has been widely used. This process and MIG welding process are not compatible on the design groove. Groove designed according to MIG welding process cannot be used for friction stir welding, so the promotion of this technology is subject to certain restrictions. However, FSW shows unique advantages as follows:
Low manufacture cost. No consumable welding materials, such as electrode, wire, flux and protective gas, are required during the welding process. Welding stirring head is the only consumption. It is not necessary to remove the oxide film before welding, which reduce cleaning time and improve production efficiency.
Good welding quality. The temperature of friction stir welding is relatively low, so the microstructure change of the heat affected zone is negligible, and the residual stress is low leading to a low deformation. The joint efficiency is high as compared to MIG weld.
Environment-friendly. The welding process is safe. There is no pollution, no smoke, no radiation, etc.
Less energy consumption. Because friction stir welding solely depends on the welding head rotation and movement, it saves more energy than fusion welding or even conventional friction welding.
High welding efficiency. It can complete the welding of long weld, large section and different position at one time.
The above advantages promote its application in rail transportation field, shown in Figure 17. It can be seen that FSW showed flat weld than MIG which requires little post-processing (Figure 18).
Figure 17.
FSW process of a sleeper beam.
Figure 18.
A comparison between MIG and FSW welds.
The defects in friction stir welding joints mainly include holes, unwelded joints, flaps and grooves. Defects are mainly caused by the fact that in the welding process, different parts of the weld metal have undergone different thermomechanical processes, and thus bring overheating or insufficient flow of plastic materials. The top of the weld is subjected to the strong friction and stirring effect of the stirring needle and the shaft shoulder at the same time, even if the welding speed is very high or the stirring head speed is not high enough, it can still ensure a certain heat input and form a defect free connection; In the middle of the weld, the heat input is less than the top, but the heat output is also less than the top and bottom, so the total heat absorption is greater than the top and bottom, and the material softening degree is the highest. The heat input at the bottom of the welding seam is the least and the output is the largest. so the welding defects will appear at the bottom of the welding seam when the process parameters are not properly selected or the size of the welding tool is not appropriate.
5.3.3 Laser-MIG welding
Laser-MIG welding is a new welding technology, which has a wide development prospect. The laser -MIG hybrid welding technology combines the laser welding technology and MIG welding technology organically, which overcomes each other\'s shortcomings, and thus favor to obtain high quality welding joint.
Laser -MIG welding uses both laser beam and arc, which has the characteristics of high welding speed, stable welding process, high thermal efficiency and allowing greater welding assembly clearance. The laser -MIG welding pool is smaller than that of MIG welding. As compared to MIG welding, laser-MIG welding shows lower heat input, smaller heat affected zone and smaller work deformation.
Based on the characteristics of concentrated heat source, strong penetration and arc wire filling welding, a new design of joint and groove of laser-MIG hybrid welding was carried out through experimental optimization and verification. Compared with the traditional MIG welding, the upper groove angle is smaller, the depth is smaller for laser-MIG hybrid welding.
The wide application range and high efficiency of laser-MIG welding enhance its competitiveness in terms of investment cost, reduced production time, reduced production cost and improved productivity. Currently It is in the stage of small-scale application in the manufacture of high speed train and relevant component, shown in Figure 19.
Figure 19.
Lase-MIG weld machine for roof of aluminum car-body of high speed train.
Figure 20 shows a comparison between MIG and laser-MIG hybrid welds. The laser-MIG hybrid welds is flat which reflect a good formability.
Figure 20.
A comparison between MIG and laser-MIG hybrid welds.
Figure 21.
Coated outer surface and internal surface of aluminum car-body.
5.4 Anti-corrosion process
The increasing operation speed of train make it experience the baptism of different environments within hours. The environmental change is quite complex when the train goes from inland to coastal cities, from high to low latitudes, from low to high altitude. The weather may change dramatically from sunny to rainy. The temperature may change from subzero to 40°C, The humidity may change from very dry to very damp (~100%). The air may change from fresh and clean to polluted. It may contain dust, oxysulfide, oxynitride, or chloridion. These ingredients would lead to corrosion to aluminum alloy which is detrimental to the safety and long-term reliability of the train, especially when it’s running at speed higher than 200 km/h.
As is known, aluminum shows good corrosion performance since it can form passive film in atmosphere. However, the corrosion resistance is also threatened by alloying elements and aggressive environmental factors. Pitting, galvanic and stress corrosion are common types of corrosion for engineering structure made of aluminum used in atmospheric environment.
For rail vehicle, an organic coating system is used to protect aluminum against corrosion. In order to deal with different environments, the coating system for outer surface is different from internal surface. The outer coating system is used to fight against harsh natural environment while the internal coating tackle the condensing water and leaking water from washing room. Therefore, it has higher requirement for the outer coating system. It needs to be evaluated by a series of accelerated corrosion test including salt spray test, damp heat test and high-low temperature test. The outer coating system consists of sand blasting pretreatment, epoxy primer, polyurethane putty, polyurethane interlayer, polyurethane finishing coat and varnish. The internal coating system consists of cleaning, etch coating, rust inhibiting primer and polyurethane top-coat. In case aluminum component joints with other alloys, a surface pre-treatment accompanied by rust inhibiting primer is necessary to ensure physical isolation from each other and against galvanic corrosion (Figure 21).
6. Conclusion
In this chapter, the latest applications of aluminum alloys in rail transportation field is introduced. The typical high-strength aluminum alloys used on high speed train was illustrated combined with the unique characteristics of aluminum alloys. The detailed application on key part of rail vehicle including car-body, gear box and axle box tie rod, were introduced. The main challenges and engineering experience were also mentioned. The key manufacturing techniques, such as casting, forming, welding, and anti-corrosion were analyzed. Hopefully, the chapter can promote the development and application of advanced materials, especially aluminum alloy, and continuously contribute to sustainable development of human civilization through technological innovation.
Acknowledgments
Authors would like to thank Mr. LIU Xuezhi, Mr. ZHANG Shilei and Mr. LI Shuaizhen from CRRC Qingdao Sifang Co., Ltd for providing figures of the chapter.
Conflict of interest
There is no conflict of interest regarding to the content of this chapter.
\n',keywords:"aluminum alloy, application, rail transportation",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/75650.pdf",chapterXML:"https://mts.intechopen.com/source/xml/75650.xml",downloadPdfUrl:"/chapter/pdf-download/75650",previewPdfUrl:"/chapter/pdf-preview/75650",totalDownloads:344,totalViews:0,totalCrossrefCites:0,dateSubmitted:"March 27th 2020",dateReviewed:"February 5th 2021",datePrePublished:"May 31st 2021",datePublished:"October 6th 2021",dateFinished:"March 10th 2021",readingETA:"0",abstract:"This chapter focus on the latest applications of aluminum alloys in rail transportation field. The typical high-strength aluminum alloys used on high speed train is introduced. The unique properties of aluminum alloys are analyzed. The detailed application is illustrated including car-body, gear box and axle box tie rod. The main challenges encountered in the application are also mentioned. The key manufacturing techniques, such as casting, forming, welding, are analyzed. Finally, the future improvement directions for better application is summarized. It is expected to set up a bridge for materials providers, equipment manufacturers and end-users, thereby promoting the advance of manufacturing technology and application of aluminum alloys in wider fields.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/75650",risUrl:"/chapter/ris/75650",signatures:"Xiaoguang Sun, Xiaohui Han, Chaofang Dong and Xiaogang Li",book:{id:"9912",type:"book",title:"Advanced Aluminium Composites and Alloys",subtitle:null,fullTitle:"Advanced Aluminium Composites and Alloys",slug:"advanced-aluminium-composites-and-alloys",publishedDate:"October 6th 2021",bookSignature:"Leszek A. 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Introduction",level:"1"},{id:"sec_2",title:"2. Characteristics of aluminum alloy",level:"1"},{id:"sec_3",title:"3. Typical aluminum alloys and their properties",level:"1"},{id:"sec_4",title:"4. Detailed application",level:"1"},{id:"sec_4_2",title:"4.1 Car-body",level:"2"},{id:"sec_5_2",title:"4.2 Gear box",level:"2"},{id:"sec_6_2",title:"4.3 Axle box",level:"2"},{id:"sec_8",title:"5. Key manufacturing techniques",level:"1"},{id:"sec_8_2",title:"5.1 Casting",level:"2"},{id:"sec_9_2",title:"5.2 Forming process",level:"2"},{id:"sec_10_2",title:"5.3 Welding",level:"2"},{id:"sec_10_3",title:"5.3.1 Arc welding MIG",level:"3"},{id:"sec_11_3",title:"5.3.2 FSW welding",level:"3"},{id:"sec_12_3",title:"5.3.3 Laser-MIG welding",level:"3"},{id:"sec_14_2",title:"5.4 Anti-corrosion process",level:"2"},{id:"sec_16",title:"6. Conclusion",level:"1"},{id:"sec_17",title:"Acknowledgments",level:"1"},{id:"sec_20",title:"Conflict of interest",level:"1"}],chapterReferences:[{id:"B1",body:'Kawajiri, K.; Kobayashi, M.; Sakamoto, K., Lightweight materials equal lightweight greenhouse gas emissions?: A historical analysis of greenhouse gases of vehicle material substitution. Journal of Cleaner Production. 2020;253:119805. DOI: 10.1016/j.jclepro.2019.119805'},{id:"B2",body:'Ashkenazi, D., How aluminum changed the world: A metallurgical revolution through technological and cultural perspectives. Technological Forecasting and Social Change, 2019; 143: 101-113. DOI: https://doi.org/10.1016/j.techfore.2019.03.011'},{id:"B3",body:'GB/T 32182-2015 Aluminium and aluminium alloy plates and sheets for railway application'},{id:"B4",body:'GB/T 26494-2011 Aluminium alloys extruded profiles used for structural material of railway vehicle carbodies'},{id:"B5",body:'Mallick, P. K., Designing lightweight vehicle body. In Materials, Design and Manufacturing for Lightweight Vehicles, 2021; pp 405-432'},{id:"B6",body:'Mallick, P. K., Joining for lightweight vehicles. In Materials, Design and Manufacturing for Lightweight Vehicles, 2021; pp 321-371'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Xiaoguang Sun",address:"sunx_sf@126.com;, sunxiaoguang@ustb.edu.cn",affiliation:'
CRRC Qingdao Sifang Co., Ltd, China
Corrosion and Protection Center, University of Science and Technology Beijing, China
Corrosion and Protection Center, University of Science and Technology Beijing, China
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The company was founded in Vienna in 2004 by Alex Lazinica and Vedran Kordic, two PhD students researching robotics. While completing our PhDs, we found it difficult to access the research we needed. So, we decided to create a new Open Access publisher. A better one, where researchers like us could find the information they needed easily. The result is IntechOpen, an Open Access publisher that puts the academic needs of the researchers before the business interests of publishers.
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We started by publishing journals and books from the fields of science we were most familiar with - AI, robotics, manufacturing and operations research. Through our growing network of institutions and authors, we soon expanded into related fields like environmental engineering, nanotechnology, computer science, renewable energy and electrical engineering, Today, we are the world’s largest Open Access publisher of scientific research, with over 4,200 books and 54,000 scientific works including peer-reviewed content from more than 116,000 scientists spanning 161 countries. Our authors range from globally-renowned Nobel Prize winners to up-and-coming researchers at the cutting edge of scientific discovery.
\\n\\n
In the same year that IntechOpen was founded, we launched what was at the time the first ever Open Access, peer-reviewed journal in its field: the International Journal of Advanced Robotic Systems (IJARS).
\\n\\n
The IntechOpen timeline
\\n\\n
2004
\\n\\n
\\n\\t
Intech Open is founded in Vienna, Austria, by Alex Lazinica and Vedran Kordic, two PhD students, and their first Open Access journals and books are published.
\\n\\t
Alex and Vedran launch the first Open Access, peer-reviewed robotics journal and IntechOpen’s flagship publication, the International Journal of Advanced Robotic Systems (IJARS).
\\n
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2005
\\n\\n
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IntechOpen publishes its first Open Access book: Cutting Edge Robotics.
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2006
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IntechOpen publishes a special issue of IJARS, featuring contributions from NASA scientists regarding the Mars Exploration Rover missions.
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2008
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Downloads milestone: 200,000 downloads reached
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2009
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Publishing milestone: the first 100 Open Access STM books are published
\\n
\\n\\n
2010
\\n\\n
\\n\\t
Downloads milestone: one million downloads reached
\\n\\t
IntechOpen expands its book publishing into a new field: medicine.
\\n
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2011
\\n\\n
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Publishing milestone: More than five million downloads reached
\\n\\t
IntechOpen publishes 1996 Nobel Prize in Chemistry winner Harold W. Kroto’s “Strategies to Successfully Cross-Link Carbon Nanotubes”. Find it here.
\\n\\t
IntechOpen and TBI collaborate on a project to explore the changing needs of researchers and the evolving ways that they discover, publish and exchange information. The result is the survey “Author Attitudes Towards Open Access Publishing: A Market Research Program”.
\\n\\t
IntechOpen hosts SHOW - Share Open Access Worldwide; a series of lectures, debates, round-tables and events to bring people together in discussion of open source principles, intellectual property, content licensing innovations, remixed and shared culture and free knowledge.
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2012
\\n\\n
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Publishing milestone: 10 million downloads reached
\\n\\t
IntechOpen holds Interact2012, a free series of workshops held by figureheads of the scientific community including Professor Hiroshi Ishiguro, director of the Intelligent Robotics Laboratory, who took the audience through some of the most impressive human-robot interactions observed in his lab.
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\\n\\n
2013
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IntechOpen joins the Committee on Publication Ethics (COPE) as part of a commitment to guaranteeing the highest standards of publishing.
\\n
\\n\\n
2014
\\n\\n
\\n\\t
IntechOpen turns 10, with more than 30 million downloads to date.
\\n\\t
IntechOpen appoints its first Regional Representatives - members of the team situated around the world dedicated to increasing the visibility of our authors’ published work within their local scientific communities.
\\n
\\n\\n
2015
\\n\\n
\\n\\t
Downloads milestone: More than 70 million downloads reached, more than doubling since the previous year.
\\n\\t
Publishing milestone: IntechOpen publishes its 2,500th book and 40,000th Open Access chapter, reaching 20,000 citations in Thomson Reuters ISI Web of Science.
\\n\\t
40 IntechOpen authors are included in the top one per cent of the world’s most-cited researchers.
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Thomson Reuters’ ISI Web of Science Book Citation Index begins indexing IntechOpen’s books in its database.
\\n
\\n\\n
2016
\\n\\n
\\n\\t
IntechOpen is identified as a world leader in Simba Information’s Open Access Book Publishing 2016-2020 report and forecast. IntechOpen came in as the world’s largest Open Access book publisher by title count.
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\\n\\n
2017
\\n\\n
\\n\\t
Downloads milestone: IntechOpen reaches more than 100 million downloads
\\n\\t
Publishing milestone: IntechOpen publishes its 3,000th Open Access book, making it the largest Open Access book collection in the world
We started by publishing journals and books from the fields of science we were most familiar with - AI, robotics, manufacturing and operations research. Through our growing network of institutions and authors, we soon expanded into related fields like environmental engineering, nanotechnology, computer science, renewable energy and electrical engineering, Today, we are the world’s largest Open Access publisher of scientific research, with over 4,200 books and 54,000 scientific works including peer-reviewed content from more than 116,000 scientists spanning 161 countries. Our authors range from globally-renowned Nobel Prize winners to up-and-coming researchers at the cutting edge of scientific discovery.
\n\n
In the same year that IntechOpen was founded, we launched what was at the time the first ever Open Access, peer-reviewed journal in its field: the International Journal of Advanced Robotic Systems (IJARS).
\n\n
The IntechOpen timeline
\n\n
2004
\n\n
\n\t
Intech Open is founded in Vienna, Austria, by Alex Lazinica and Vedran Kordic, two PhD students, and their first Open Access journals and books are published.
\n\t
Alex and Vedran launch the first Open Access, peer-reviewed robotics journal and IntechOpen’s flagship publication, the International Journal of Advanced Robotic Systems (IJARS).
\n
\n\n
2005
\n\n
\n\t
IntechOpen publishes its first Open Access book: Cutting Edge Robotics.
\n
\n\n
2006
\n\n
\n\t
IntechOpen publishes a special issue of IJARS, featuring contributions from NASA scientists regarding the Mars Exploration Rover missions.
\n
\n\n
2008
\n\n
\n\t
Downloads milestone: 200,000 downloads reached
\n
\n\n
2009
\n\n
\n\t
Publishing milestone: the first 100 Open Access STM books are published
\n
\n\n
2010
\n\n
\n\t
Downloads milestone: one million downloads reached
\n\t
IntechOpen expands its book publishing into a new field: medicine.
\n
\n\n
2011
\n\n
\n\t
Publishing milestone: More than five million downloads reached
\n\t
IntechOpen publishes 1996 Nobel Prize in Chemistry winner Harold W. Kroto’s “Strategies to Successfully Cross-Link Carbon Nanotubes”. Find it here.
\n\t
IntechOpen and TBI collaborate on a project to explore the changing needs of researchers and the evolving ways that they discover, publish and exchange information. The result is the survey “Author Attitudes Towards Open Access Publishing: A Market Research Program”.
\n\t
IntechOpen hosts SHOW - Share Open Access Worldwide; a series of lectures, debates, round-tables and events to bring people together in discussion of open source principles, intellectual property, content licensing innovations, remixed and shared culture and free knowledge.
\n
\n\n
2012
\n\n
\n\t
Publishing milestone: 10 million downloads reached
\n\t
IntechOpen holds Interact2012, a free series of workshops held by figureheads of the scientific community including Professor Hiroshi Ishiguro, director of the Intelligent Robotics Laboratory, who took the audience through some of the most impressive human-robot interactions observed in his lab.
\n
\n\n
2013
\n\n
\n\t
IntechOpen joins the Committee on Publication Ethics (COPE) as part of a commitment to guaranteeing the highest standards of publishing.
\n
\n\n
2014
\n\n
\n\t
IntechOpen turns 10, with more than 30 million downloads to date.
\n\t
IntechOpen appoints its first Regional Representatives - members of the team situated around the world dedicated to increasing the visibility of our authors’ published work within their local scientific communities.
\n
\n\n
2015
\n\n
\n\t
Downloads milestone: More than 70 million downloads reached, more than doubling since the previous year.
\n\t
Publishing milestone: IntechOpen publishes its 2,500th book and 40,000th Open Access chapter, reaching 20,000 citations in Thomson Reuters ISI Web of Science.
\n\t
40 IntechOpen authors are included in the top one per cent of the world’s most-cited researchers.
\n\t
Thomson Reuters’ ISI Web of Science Book Citation Index begins indexing IntechOpen’s books in its database.
\n
\n\n
2016
\n\n
\n\t
IntechOpen is identified as a world leader in Simba Information’s Open Access Book Publishing 2016-2020 report and forecast. IntechOpen came in as the world’s largest Open Access book publisher by title count.
\n
\n\n
2017
\n\n
\n\t
Downloads milestone: IntechOpen reaches more than 100 million downloads
\n\t
Publishing milestone: IntechOpen publishes its 3,000th Open Access book, making it the largest Open Access book collection in the world
\n
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Shohel"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},subject:{topic:{id:"300",title:"Genesiology",slug:"genesiology",parent:{id:"25",title:"Veterinary Medicine and Science",slug:"veterinary-medicine-and-science"},numberOfBooks:2,numberOfSeries:0,numberOfAuthorsAndEditors:73,numberOfWosCitations:52,numberOfCrossrefCitations:34,numberOfDimensionsCitations:79,videoUrl:null,fallbackUrl:null,description:null},booksByTopicFilter:{topicId:"300",sort:"-publishedDate",limit:12,offset:0},booksByTopicCollection:[{type:"book",id:"7233",title:"New Insights into Theriogenology",subtitle:null,isOpenForSubmission:!1,hash:"74f4147e3fb214dd050e5edd3aaf53bc",slug:"new-insights-into-theriogenology",bookSignature:"Rita Payan-Carreira",coverURL:"https://cdn.intechopen.com/books/images_new/7233.jpg",editedByType:"Edited by",editors:[{id:"38652",title:"Dr.",name:"Rita",middleName:null,surname:"Payan-Carreira",slug:"rita-payan-carreira",fullName:"Rita Payan-Carreira"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5105",title:"Insights from Animal Reproduction",subtitle:null,isOpenForSubmission:!1,hash:"25cd16b683d1f098bc304cbbdb3206cd",slug:"insights-from-animal-reproduction",bookSignature:"Rita Payan Carreira",coverURL:"https://cdn.intechopen.com/books/images_new/5105.jpg",editedByType:"Edited by",editors:[{id:"38652",title:"Dr.",name:"Rita",middleName:null,surname:"Payan-Carreira",slug:"rita-payan-carreira",fullName:"Rita Payan-Carreira"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:2,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"49736",doi:"10.5772/62053",title:"Chromosome Abnormalities in Domestic Animals as Causes of Disorders of Sex Development or Impaired Fertility",slug:"chromosome-abnormalities-in-domestic-animals-as-causes-of-disorders-of-sex-development-or-impaired-f",totalDownloads:4132,totalCrossrefCites:7,totalDimensionsCites:20,abstract:"Cytogenetic evaluation is an important step in the diagnosis of infertile or sterile animals. Moreover, the analysis of sex chromosomes is crucial for a proper classification of disorders of sex development (DSD). For many years, chromosome studies mainly addressed the livestock species, while recently, increasing interest in such analysis in companion animals is observed. New molecular and cytogenetic tools and techniques have given opportunities for a precise identification of chromosome mutations. Among them, fluorescence in situ hybridization, besides chromosome banding, has become a gold standard. In this chapter, recent advances in the cytogenetic diagnosis of cattle, pigs, horses, dogs and cats are presented.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Izabela Szczerbal and Marek Switonski",authors:[{id:"177030",title:"Prof.",name:"Marek",middleName:null,surname:"Switonski",slug:"marek-switonski",fullName:"Marek Switonski"},{id:"177045",title:"Dr.",name:"Izabela",middleName:null,surname:"Szczerbal",slug:"izabela-szczerbal",fullName:"Izabela Szczerbal"}]},{id:"49857",doi:"10.5772/62207",title:"Germ Cell Determinant Transmission, Segregation, and Function in the Zebrafish Embryo",slug:"germ-cell-determinant-transmission-segregation-and-function-in-the-zebrafish-embryo",totalDownloads:2278,totalCrossrefCites:4,totalDimensionsCites:11,abstract:"Animals specify primordial germ cells (PGCs) in two alternate modes: preformation and epigenesis. Epigenesis relies on signal transduction from the surrounding tissues to instruct a group of cells to acquire PGC identity. Preformation, thought to be the more derived PGC specification mode, is instead based on the maternal inheritance of germ cell-determining factors. We use the zebrafish as a model system, in which PGCs are specified through maternal inheritance of germ plasm, to study this process in vertebrates. In zebrafish, maternally inherited germ plasm ribonucleoparticles (RNPs) have co-opted the cytoskeletal machinery to reach progressive levels of multimerization, resulting in the formation of four large masses of aggregated germ plasm RNPs. At later stages, germ plasm masses continue to use components of the cell division machinery, such as the spindles, centrosomes, and/or subcellular organelles to segregate asymmetrically during cell division and subsequently induce germ cell fate. This chapter discusses the current knowledge of germ cell specification focusing on the zebrafish as a model system. We also provide a comparative analysis of the mechanism for germ plasm RNP segregation in zebrafish versus other known vertebrate systems of germ cell preformation, such as in amphibian and avian models.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Celeste Eno and Francisco Pelegri",authors:[{id:"177209",title:"Prof.",name:"Francisco",middleName:null,surname:"Pelegri",slug:"francisco-pelegri",fullName:"Francisco Pelegri"}]},{id:"62171",doi:"10.5772/intechopen.79106",title:"Intraoviductal Instillation of a Solution as an Effective Route for Manipulating Preimplantation Mammalian Embryos in vivo",slug:"intraoviductal-instillation-of-a-solution-as-an-effective-route-for-manipulating-preimplantation-mam",totalDownloads:1124,totalCrossrefCites:8,totalDimensionsCites:9,abstract:"Preimplantation embryos of mammals are enclosed by a translucent layer called zona pellucida (ZP), which is composed of glycoproteins. ZP is important for protecting against infection by virus and bacteria, and to prevent attachment of embryos to the oviductal epithelia. Due to the presence of ZP, it has been difficult to transfect preimplantation embryos existing within the oviductal lumen, with exogenous nucleic acids, such as DNA and mRNA. However, intraoviductal instillation of nucleic acids, and subsequent in vivo electroporation in pregnant females, enables transfection of these embryos, leading to the production of gene-modified animals. This new method for production of genetically modified animals does not require any ex vivo handling of embryos, which has been essential for traditional transgenesis. In this article, we describe recent advances in the in vivo transfection of preimplantation mammalian embryos, and also the possibility of simple transfection of these embryos through intraoviductal instillation of a solution, alone.",book:{id:"7233",slug:"new-insights-into-theriogenology",title:"New Insights into Theriogenology",fullTitle:"New Insights into Theriogenology"},signatures:"Masahiro Sato, Masato Ohtsuka and Shingo Nakamura",authors:[{id:"177440",title:"Dr.",name:"Masato",middleName:null,surname:"Ohtsuka",slug:"masato-ohtsuka",fullName:"Masato Ohtsuka"},{id:"177444",title:"Dr.",name:"Shingo",middleName:null,surname:"Nakamura",slug:"shingo-nakamura",fullName:"Shingo Nakamura"},{id:"245795",title:"Prof.",name:"Masahiro",middleName:null,surname:"Sato",slug:"masahiro-sato",fullName:"Masahiro Sato"}]},{id:"50061",doi:"10.5772/62470",title:"Sperm Motility Regulatory Proteins: A Tool to Enhance Sperm Quality",slug:"sperm-motility-regulatory-proteins-a-tool-to-enhance-sperm-quality",totalDownloads:2126,totalCrossrefCites:2,totalDimensionsCites:6,abstract:"Sperm forward motility is an essential parameter in mammalian fertilization. Studies from our laboratory have identified and characterized a few unique sperm motility regulatory proteins/glycoproteins from the male reproductive fluids and mammalian blood serum. The purified sperm motility-initiating protein (MIP) from caprine epididymal plasma as well as the forward motility-stimulating factor (FMSF) and motility-stimulating protein (MSP) from buffalo and goat serum, respectively, have high efficacy to initiate or increase motility in nonmotile or less motile sperm. Antibody of sperm motility inhibitory factor (MIF-II) has the high potential to enhance sperm vertical velocity and forward motility by increasing intracellular cyclic adenosine monophosphate (cAMP) level. The appearance and disappearance of D-galactose–specific lectin and its receptor along the epididymis has been reported to be involved in motility regulation in spermatozoa. A novel synthetic cryopreservation method and role of lipid to protect membrane damage during cryopreservation have been demonstrated. Motility-promoting proteins may be extremely useful for improving cattle breeding and breeding of endangered species, thereby helping in enhanced production of animal products as well as in the conservation of animals. Isolated proteins and developed cryopreservation technology may also be beneficial in human infertility clinics to increase the chance of fertilization.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Sandhya R. Dungdung, Arpita Bhoumik, Sudipta Saha, Prasanta\nGhosh, Kaushik Das, Sandipan Mukherjee, Debjani Nath, Jitamanyu\nChakrabarty, Chanakyanath Kundu, Bijay Shankar Jaiswal, Mahitosh\nMandal, Arunima Maiti, Saswati Banerjee, Madhumita\nRoychowdhury, Debleena Ray, Debdas Bhattacharyya and Gopal C.\nMajumder",authors:[{id:"50052",title:"Dr.",name:"Mahitosh",middleName:null,surname:"Mandal",slug:"mahitosh-mandal",fullName:"Mahitosh Mandal"},{id:"177044",title:"Dr.",name:"Sandhya",middleName:null,surname:"Dungdung",slug:"sandhya-dungdung",fullName:"Sandhya Dungdung"},{id:"177920",title:"Dr.",name:"Arpita",middleName:null,surname:"Bhoumik",slug:"arpita-bhoumik",fullName:"Arpita Bhoumik"},{id:"177921",title:"Dr.",name:"Sudipta",middleName:null,surname:"Saha",slug:"sudipta-saha",fullName:"Sudipta Saha"},{id:"177922",title:"MSc.",name:"Prasanta",middleName:null,surname:"Ghosh",slug:"prasanta-ghosh",fullName:"Prasanta Ghosh"},{id:"177923",title:"Dr.",name:"Kaushik",middleName:null,surname:"Das",slug:"kaushik-das",fullName:"Kaushik Das"},{id:"177924",title:"MSc.",name:"Sandipan",middleName:null,surname:"Mukherjee",slug:"sandipan-mukherjee",fullName:"Sandipan Mukherjee"},{id:"177925",title:"Dr.",name:"Debjani",middleName:null,surname:"Nath",slug:"debjani-nath",fullName:"Debjani Nath"},{id:"177927",title:"Dr.",name:"Jitamanyu",middleName:null,surname:"Chakrabarty",slug:"jitamanyu-chakrabarty",fullName:"Jitamanyu Chakrabarty"},{id:"177928",title:"Dr.",name:"Chanakyanath",middleName:null,surname:"Kundu",slug:"chanakyanath-kundu",fullName:"Chanakyanath Kundu"},{id:"177929",title:"Dr.",name:"Bijay Shankar",middleName:null,surname:"Jaiswal",slug:"bijay-shankar-jaiswal",fullName:"Bijay Shankar Jaiswal"},{id:"177930",title:"Dr.",name:"Arunima",middleName:null,surname:"Maiti",slug:"arunima-maiti",fullName:"Arunima Maiti"},{id:"177931",title:"Dr.",name:"Saswati",middleName:null,surname:"Banerjee",slug:"saswati-banerjee",fullName:"Saswati Banerjee"},{id:"177932",title:"Dr.",name:"Madhumita",middleName:null,surname:"Roychowdhury",slug:"madhumita-roychowdhury",fullName:"Madhumita Roychowdhury"},{id:"177933",title:"MSc.",name:"Debleena",middleName:null,surname:"Ray",slug:"debleena-ray",fullName:"Debleena Ray"},{id:"177934",title:"Dr.",name:"Debdas",middleName:null,surname:"Bhattacharyya",slug:"debdas-bhattacharyya",fullName:"Debdas Bhattacharyya"},{id:"177935",title:"Dr.",name:"Gopal Chandra",middleName:null,surname:"Majumder",slug:"gopal-chandra-majumder",fullName:"Gopal Chandra Majumder"}]},{id:"63404",doi:"10.5772/intechopen.80229",title:"Subclinical Endometritis in Dairy Cattle",slug:"subclinical-endometritis-in-dairy-cattle",totalDownloads:1800,totalCrossrefCites:1,totalDimensionsCites:5,abstract:"Subclinical endometritis is recognized as a cause of poor reproductive performance in dairy cows. Inflammation of the endometrium persisting after postpartum uterine involution has been related with prolonged calving-conception intervals and low fertility in dairy cows. The subclinical nature of this condition makes it necessary in the use of endometrial cytology or biopsy for diagnosing it. There are some controversies among authors in relation to the postpartum period from which a physiological endometrial inflammation should be considered a pathological subclinical endometritis. Therefore, depending on the sampling period after calving, different studies establish a different degree of polymorphonuclear leukocyte infiltration as cutoff point to diagnose subclinical endometritis. Controversies also exist regarding the pathogenesis of the disease and its consequences on the fertility of dairy cattle. The aim of this chapter was to review the current knowledge on this uterine pathology.",book:{id:"7233",slug:"new-insights-into-theriogenology",title:"New Insights into Theriogenology",fullTitle:"New Insights into Theriogenology"},signatures:"Luis Angel Quintela Arias, Marcos Vigo Fernández, Juan José\nBecerra González, Mónica Barrio López, Pedro José García Herradón\nand Ana Isabel Peña Martínez",authors:[{id:"243272",title:"Prof.",name:"Luis Angel",middleName:null,surname:"Quintela Arias",slug:"luis-angel-quintela-arias",fullName:"Luis Angel Quintela Arias"},{id:"243886",title:"Prof.",name:"Ana Isabel",middleName:null,surname:"Peña Martínez",slug:"ana-isabel-pena-martinez",fullName:"Ana Isabel Peña Martínez"},{id:"243887",title:"Prof.",name:"Pedro",middleName:null,surname:"García Herradón",slug:"pedro-garcia-herradon",fullName:"Pedro García Herradón"},{id:"243888",title:"Prof.",name:"Juan José",middleName:null,surname:"Becerra González",slug:"juan-jose-becerra-gonzalez",fullName:"Juan José Becerra González"},{id:"256852",title:"Dr.",name:"Mónica",middleName:null,surname:"Barrio López",slug:"monica-barrio-lopez",fullName:"Mónica Barrio López"},{id:"256854",title:"Dr.",name:"Marcos",middleName:null,surname:"Vigo Fernández",slug:"marcos-vigo-fernandez",fullName:"Marcos Vigo Fernández"}]}],mostDownloadedChaptersLast30Days:[{id:"49736",title:"Chromosome Abnormalities in Domestic Animals as Causes of Disorders of Sex Development or Impaired Fertility",slug:"chromosome-abnormalities-in-domestic-animals-as-causes-of-disorders-of-sex-development-or-impaired-f",totalDownloads:4132,totalCrossrefCites:7,totalDimensionsCites:20,abstract:"Cytogenetic evaluation is an important step in the diagnosis of infertile or sterile animals. Moreover, the analysis of sex chromosomes is crucial for a proper classification of disorders of sex development (DSD). For many years, chromosome studies mainly addressed the livestock species, while recently, increasing interest in such analysis in companion animals is observed. New molecular and cytogenetic tools and techniques have given opportunities for a precise identification of chromosome mutations. Among them, fluorescence in situ hybridization, besides chromosome banding, has become a gold standard. In this chapter, recent advances in the cytogenetic diagnosis of cattle, pigs, horses, dogs and cats are presented.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Izabela Szczerbal and Marek Switonski",authors:[{id:"177030",title:"Prof.",name:"Marek",middleName:null,surname:"Switonski",slug:"marek-switonski",fullName:"Marek Switonski"},{id:"177045",title:"Dr.",name:"Izabela",middleName:null,surname:"Szczerbal",slug:"izabela-szczerbal",fullName:"Izabela Szczerbal"}]},{id:"50144",title:"Proliferative Endometrial Lesions Hidden behind the Feline Pyometra",slug:"proliferative-endometrial-lesions-hidden-behind-the-feline-pyometra",totalDownloads:2456,totalCrossrefCites:0,totalDimensionsCites:4,abstract:"The literature refers to pyometra as the most important pathology in the feline uterus, which is often associated with cystic endometrial disease (cystic endometrial hyperplasia/pyometra complex or CEH-Pyo). The etiology of pyometra is complex and probably multifactorial, but hormonal influences are suggested to play an important role in the pathogenesis. Progestagen-based contraceptives may be risk factors for the CEH-Pyo syndrome, for endometrial adenocarcinoma and also to mammary tumors in this species.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Maria dos Anjos Pires, Hugo Vilhena, Sónia Miranda, Miguel\nTavares Pereira, Fernanda Seixas and Ana Laura Saraiva",authors:[{id:"41065",title:"Dr.",name:"Sónia",middleName:null,surname:"Miranda",slug:"sonia-miranda",fullName:"Sónia Miranda"},{id:"161556",title:"Dr.",name:"Maria Dos Anjos",middleName:null,surname:"Pires",slug:"maria-dos-anjos-pires",fullName:"Maria Dos Anjos Pires"},{id:"179547",title:"MSc.",name:"Hugo",middleName:null,surname:"Vilhena",slug:"hugo-vilhena",fullName:"Hugo Vilhena"},{id:"179548",title:"MSc.",name:"Miguel",middleName:null,surname:"Tavares Pereira",slug:"miguel-tavares-pereira",fullName:"Miguel Tavares Pereira"},{id:"179549",title:"Prof.",name:"Fernanda",middleName:null,surname:"Seixas",slug:"fernanda-seixas",fullName:"Fernanda Seixas"},{id:"179550",title:"Prof.",name:"Ana Laura",middleName:null,surname:"Saraiva",slug:"ana-laura-saraiva",fullName:"Ana Laura Saraiva"}]},{id:"49944",title:"The Use of Reproductive Technologies to Produce Transgenic Goats",slug:"the-use-of-reproductive-technologies-to-produce-transgenic-goats",totalDownloads:2411,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"Recombinant DNA technology has revolutionized the production of therapeutic proteins. Thus, genes of a great number of human proteins have already been identified and cloned. The use of farm animals as bioreactors may be the better choice to produce recombinant therapeutic proteins. For this activity, the term “pharming” was created, referring to the use of genetic engineering to obtain a transgenic or genetically modified animal. Considering the advantages and disadvantages of livestock species, goats appear as a very good model. In addition, the first human commercially approved biological drug (antithrombin (AT)) was produced from the milk of transgenic goats. The aim of this chapter is to present various reproductive technologies used to obtain transgenic goats secreting recombinant proteins in milk. Initially, this chapter presents the methods for embryo production (in vivo and in vitro) to realize the DNA microinjection in pronuclear embryos. Thus, the techniques of superovulation of donors (in vivo embryo production) and ovarian stimulation for oocyte recovery (in vitro embryo production) are described. Also, the methods for DNA microinjection and embryo transfer are detailed in this chapter. Finally, this chapter describes the reproductive procedures used for obtaining transgenic goats by cloning.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Vicente J. F. Freitas, Luciana M. Melo, Dárcio I.A. Teixeira, Maajid H.\nBhat, Irina A. Serova, Lyudmila E. Andreeva and Oleg L. Serov",authors:[{id:"177122",title:"Dr.",name:"Vicente",middleName:null,surname:"Freitas",slug:"vicente-freitas",fullName:"Vicente Freitas"},{id:"177194",title:"Dr.",name:"Luciana",middleName:null,surname:"Melo",slug:"luciana-melo",fullName:"Luciana Melo"},{id:"177195",title:"Dr.",name:"Dárcio",middleName:null,surname:"Teixeira",slug:"darcio-teixeira",fullName:"Dárcio Teixeira"},{id:"177196",title:"Dr.",name:"Maajid",middleName:null,surname:"Bhat",slug:"maajid-bhat",fullName:"Maajid Bhat"},{id:"185365",title:"Dr.",name:"Irina",middleName:null,surname:"Aleksandrovna SEROVA",slug:"irina-aleksandrovna-serova",fullName:"Irina Aleksandrovna SEROVA"},{id:"185366",title:"Dr.",name:"Lyudmila",middleName:null,surname:"Evgenievna ANDREEVA",slug:"lyudmila-evgenievna-andreeva",fullName:"Lyudmila Evgenievna ANDREEVA"},{id:"185367",title:"Dr.",name:"Oleg",middleName:null,surname:"Leonidovich SEROV",slug:"oleg-leonidovich-serov",fullName:"Oleg Leonidovich SEROV"}]},{id:"63404",title:"Subclinical Endometritis in Dairy Cattle",slug:"subclinical-endometritis-in-dairy-cattle",totalDownloads:1800,totalCrossrefCites:1,totalDimensionsCites:5,abstract:"Subclinical endometritis is recognized as a cause of poor reproductive performance in dairy cows. Inflammation of the endometrium persisting after postpartum uterine involution has been related with prolonged calving-conception intervals and low fertility in dairy cows. The subclinical nature of this condition makes it necessary in the use of endometrial cytology or biopsy for diagnosing it. There are some controversies among authors in relation to the postpartum period from which a physiological endometrial inflammation should be considered a pathological subclinical endometritis. Therefore, depending on the sampling period after calving, different studies establish a different degree of polymorphonuclear leukocyte infiltration as cutoff point to diagnose subclinical endometritis. Controversies also exist regarding the pathogenesis of the disease and its consequences on the fertility of dairy cattle. The aim of this chapter was to review the current knowledge on this uterine pathology.",book:{id:"7233",slug:"new-insights-into-theriogenology",title:"New Insights into Theriogenology",fullTitle:"New Insights into Theriogenology"},signatures:"Luis Angel Quintela Arias, Marcos Vigo Fernández, Juan José\nBecerra González, Mónica Barrio López, Pedro José García Herradón\nand Ana Isabel Peña Martínez",authors:[{id:"243272",title:"Prof.",name:"Luis Angel",middleName:null,surname:"Quintela Arias",slug:"luis-angel-quintela-arias",fullName:"Luis Angel Quintela Arias"},{id:"243886",title:"Prof.",name:"Ana Isabel",middleName:null,surname:"Peña Martínez",slug:"ana-isabel-pena-martinez",fullName:"Ana Isabel Peña Martínez"},{id:"243887",title:"Prof.",name:"Pedro",middleName:null,surname:"García Herradón",slug:"pedro-garcia-herradon",fullName:"Pedro García Herradón"},{id:"243888",title:"Prof.",name:"Juan José",middleName:null,surname:"Becerra González",slug:"juan-jose-becerra-gonzalez",fullName:"Juan José Becerra González"},{id:"256852",title:"Dr.",name:"Mónica",middleName:null,surname:"Barrio López",slug:"monica-barrio-lopez",fullName:"Mónica Barrio López"},{id:"256854",title:"Dr.",name:"Marcos",middleName:null,surname:"Vigo Fernández",slug:"marcos-vigo-fernandez",fullName:"Marcos Vigo Fernández"}]},{id:"79909",title:"Cryopreservation Methods and Frontiers in the Art of Freezing Life in Animal Models",slug:"cryopreservation-methods-and-frontiers-in-the-art-of-freezing-life-in-animal-models",totalDownloads:159,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"The development in cryobiology in animal breeding had revolutionized the field of reproductive medicine. The main objective to preserve animal germplasm stems from variety of reasons such as conservation of endangered animal species, animal diversity, and an increased demand of animal models and/or genetically modified animals for research involving animal and human diseases. Cryopreservation has emerged as promising technique for fertility preservation and assisted reproduction techniques (ART) for production of animal breeds and genetically engineered animal species for research. Slow rate freezing and rapid freezing/vitrification are the two main methods of cryopreservation. Slow freezing is characterized by the phase transition (liquid turning into solid) when reducing the temperature below freezing point. Vitrification, on the other hand, is a phenomenon in which liquid solidifies without the formation of ice crystals, thus the process is referred to as a glass transition or ice-free cryopreservation. The vitrification protocol applies high concentrations of cryoprotective agents (CPA) used to avoid cryoinjury. This chapter provides a brief overview of fundamentals of cryopreservation and established methods adopted in cryopreservation. Strategies involved in cryopreserving germ cells (sperm and egg freezing) are included in this chapter. Last section describes the frontiers and advancement of cryopreservation in some of the important animal models like rodents (mouse and rats) and in few large animals (sheep, cow etc).",book:{id:"10664",slug:null,title:"Animal Reproduction",fullTitle:"Animal Reproduction"},signatures:"Feda S. Aljaser",authors:null}],onlineFirstChaptersFilter:{topicId:"300",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"79909",title:"Cryopreservation Methods and Frontiers in the Art of Freezing Life in Animal Models",slug:"cryopreservation-methods-and-frontiers-in-the-art-of-freezing-life-in-animal-models",totalDownloads:167,totalDimensionsCites:0,doi:"10.5772/intechopen.101750",abstract:"The development in cryobiology in animal breeding had revolutionized the field of reproductive medicine. The main objective to preserve animal germplasm stems from variety of reasons such as conservation of endangered animal species, animal diversity, and an increased demand of animal models and/or genetically modified animals for research involving animal and human diseases. Cryopreservation has emerged as promising technique for fertility preservation and assisted reproduction techniques (ART) for production of animal breeds and genetically engineered animal species for research. Slow rate freezing and rapid freezing/vitrification are the two main methods of cryopreservation. Slow freezing is characterized by the phase transition (liquid turning into solid) when reducing the temperature below freezing point. Vitrification, on the other hand, is a phenomenon in which liquid solidifies without the formation of ice crystals, thus the process is referred to as a glass transition or ice-free cryopreservation. The vitrification protocol applies high concentrations of cryoprotective agents (CPA) used to avoid cryoinjury. This chapter provides a brief overview of fundamentals of cryopreservation and established methods adopted in cryopreservation. Strategies involved in cryopreserving germ cells (sperm and egg freezing) are included in this chapter. Last section describes the frontiers and advancement of cryopreservation in some of the important animal models like rodents (mouse and rats) and in few large animals (sheep, cow etc).",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Feda S. Aljaser"},{id:"79782",title:"Avian Reproduction",slug:"avian-reproduction",totalDownloads:151,totalDimensionsCites:0,doi:"10.5772/intechopen.101185",abstract:"There are about 10,400 living avian species belonging to the class Aves, characterized by feathers which no other animal classes possess and are warm-blooded vertebrates with four-chamber heart. They have excellent vision, and their forelimbs are modified into wings for flight or swimming, though not all can fly or swim. They lay hard-shelled eggs which are a secretory product of the reproductive system that vary greatly in colour, shape and size, and the bigger the bird, the bigger the egg. Since domestication, avian species have been basically reared for eggs, meat, pleasure and research. They reproduce sexually with the spermatozoa being homogametic and carry Z-bearing chromosomes, and the blastodisk carries either Z-bearing or W-bearing chromosomes, hence, the female is heterogametic, and thus, determines the sex of the offspring. The paired testes produce spermatozoa, sex hormones and the single ovary (with a few exceptions) produces yolk bearing the blastodisk and sex hormones. Both testis and ovary are the primary sex organs involved in sexual characteristics development in avian. In avian reproduction, there must be mating for fertile egg that must be incubated to produce the young ones. At hatch, hatchling sex is identified and reared to meet the aim of the farmer.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Kingsley Omogiade Idahor"},{id:"78802",title:"Intraovarian Gestation in Viviparous Teleosts: Unique Type of Gestation among Vertebrates",slug:"intraovarian-gestation-in-viviparous-teleosts-unique-type-of-gestation-among-vertebrates",totalDownloads:184,totalDimensionsCites:0,doi:"10.5772/intechopen.100267",abstract:"The intraovarian gestation, occurring in teleosts, makes this type of reproduction a such complex and unique condition among vertebrates. This type of gestation of teleosts is expressed in special morphological and physiological characteristic where occurs the viviparity and it is an essential component in the analysis of the evolutionary process of viviparity in vertebrates. In viviparous teleosts, during embryogenesis, there are not development of Müllerian ducts, which form the oviducts in the rest of vertebrates, as a result, exclusively in teleosts, there are not oviducts and the caudal region of the ovary, the gonoduct, connects the ovary to the exterior. The lack of oviducts defines that the embryos develop into the ovary, as intraovarian gestation. The ovary forms the oocytes which may develop different type of oogenesis, according with the storage of diverse amount of yolk, variation observed corresponding to the species. The viviparous gestation is characterized by the possible intimate contact between maternal and embryonic tissues, process that permits their metabolic interchanges. So, the nutrients obtained by the embryos could be deposited in the oocyte before fertilization, contained in the yolk (lecithotrophy), and may be completed during gestation by additional provisioning from maternal tissues to the embryo (matrotrophy). Then, essential requirements for viviparity in poeciliids and goodeids are characterized by: a) the diversification of oogenesis, with the deposition of different amount of yolk in the oocyte; b) the insemination, by the transfer of sperm to the female gonoduct and their transportation from the gonoduct to the germinal region of the ovary where the follicles develop; c) the intrafollicular fertilization; d) the intraovarian gestation with the development of embryos in intrafollicular gestation (as in poeciliids), or intraluminal gestation (as in goodeids); and, e) the origin of embryonic nutrition may be by lecithotrophy and matrotrophy. The focus of this revision compares the general and specific structural characteristics of the viviparity occurring into the intraovarian gestation in teleosts, defining this reproductive strategy, illustrated in this review with histological material in a poeciliid, of the species Poecilia latipinna (Lesueur, 1821) (Poeciliidae), and in a goodeid, of the species Xenotoca eiseni (Rutter, 1896) (Goodeidae).",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Mari-Carmen Uribe, Gabino De la Rosa-Cruz, Adriana García-Alarcón and Juan Carlos Campuzano-Caballero"},{id:"78617",title:"Doppler Ultrasound in the Reproduction of Mares",slug:"doppler-ultrasound-in-the-reproduction-of-mares",totalDownloads:123,totalDimensionsCites:0,doi:"10.5772/intechopen.98951",abstract:"Doppler ultrasonographic (US) is a method that provides real-time information on vascular architecture and hemodynamic aspects of blood vessels. It can determine the presence, direction, and speed of blood flow, being subdivided into the categories of color Doppler (color flow and power flow) and pulsed Doppler. The objective of this chapter was to compile data from several studies addressing the use of US Doppler correlated with pathophysiological phenomena of equine reproduction. Initially we decided to describe the technique, advantages, and disadvantages of each Doppler mode. Then the applicability of US Doppler in mares related to equine reproduction. Thus, within this chapter, you will find the form of use and descriptions of studies carried out on vascular perfusion of the follicular dynamics, the corpus luteum, the uterine segments, which we have divided into post-insemination evaluation, endometritis diagnosis and pregnancy diagnosis. So, we hope that this chapter will expand the knowledge about US Doppler and increase the number of veterinarians who will introduce the technique into their practical routine.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Camila Silva Costa Ferreira and Rita de Cássia Lima Morais"},{id:"78202",title:"Stimulatory Effects of Androgens on Eel Primary Ovarian Development - from Phenotypes to Genotypes",slug:"stimulatory-effects-of-androgens-on-eel-primary-ovarian-development-from-phenotypes-to-genotypes",totalDownloads:141,totalDimensionsCites:0,doi:"10.5772/intechopen.99582",abstract:"Androgens stimulate primary ovarian development in Vertebrate. Japanese eels underwent operation to sample the pre- and post-treated ovarian tissues from the same individual. Ovarian phenotypic or genotypic data were mined in a pair. A correlation between the initial ovarian status (determined by kernel density estimation (KDE), presented as a probability density of oocyte size) and the consequence of androgen (17MT) treatment (change in ovary) has been showed. The initial ovarian status appeared to be important to influence ovarian androgenic sensitivity. The initial ovary was important to the outcomes of androgen treatments, and ePAV (expression presence-absence variation) is existing in Japanese eel by analyze DEGs; core, unique, or accessory genes were identified, the sensitivities of initial ovaries were correlated with their gene expression profiles. We speculated the importance of genetic differential expression on the variations of phenotypes by 17MT, and transcriptomic approach seems to allow extracting multiple layers of genomic data.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Yung-Sen Huang and Chung-Yen Lin"},{id:"78116",title:"Embryo Transfer",slug:"embryo-transfer",totalDownloads:258,totalDimensionsCites:1,doi:"10.5772/intechopen.99683",abstract:"Assisted reproductive technologies (ART) have made tremendous advances, in last years. Artificial insemination is a method for achieving slow genetic progress in populations of animals. Many large and small ruminants are bred by AI, and more than a half million embryos are transferred every year around the world. Most of the ruminants sires used for artificial insemination were derived from embryo transfer. Improvements of reproductive biotechnologies of controlling the estrous cycle and ovulation have resulted in more effective programs for AI, superovulation of donor, and the management of ET. In the ruminants, ET procedure is a timely alternative that can allow good conception rates to be obtained constant in a year. There have been great advances of this biotechnique with on aimed to intensify the genetic progress between generations of farm. The gains is possible with the development of advanced reproductive biotechnique. The best current strategy in applying biotechnology to farmers is to use AI with sexed semen, so farmers will enjoy and benefit. The use of ET together with cryopreserved sexed embryos has a very specific potential for donor replacement and genetic improvement of the herd. In this chapter, procedures of the MOET protocol were described step by step.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Ștefan Gregore Ciornei"}],onlineFirstChaptersTotal:7},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:98,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:287,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:10,numberOfPublishedChapters:103,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:10,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"24",title:"Sustainable Development",doi:"10.5772/intechopen.100361",issn:null,scope:"
\r\n\tTransforming our World: the 2030 Agenda for Sustainable Development endorsed by United Nations and 193 Member States, came into effect on Jan 1, 2016, to guide decision making and actions to the year 2030 and beyond. Central to this Agenda are 17 Goals, 169 associated targets and over 230 indicators that are reviewed annually. The vision envisaged in the implementation of the SDGs is centered on the five Ps: People, Planet, Prosperity, Peace and Partnership. This call for renewed focused efforts ensure we have a safe and healthy planet for current and future generations.
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\r\n\tThis Series focuses on covering research and applied research involving the five Ps through the following topics:
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\r\n\t1. Sustainable Economy and Fair Society that relates to SDG 1 on No Poverty, SDG 2 on Zero Hunger, SDG 8 on Decent Work and Economic Growth, SDG 10 on Reduced Inequalities, SDG 12 on Responsible Consumption and Production, and SDG 17 Partnership for the Goals
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\r\n\t2. Health and Wellbeing focusing on SDG 3 on Good Health and Wellbeing and SDG 6 on Clean Water and Sanitation
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\r\n\t3. Inclusivity and Social Equality involving SDG 4 on Quality Education, SDG 5 on Gender Equality, and SDG 16 on Peace, Justice and Strong Institutions
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\r\n\t
\r\n
\r\n\t4. Climate Change and Environmental Sustainability comprising SDG 13 on Climate Action, SDG 14 on Life Below Water, and SDG 15 on Life on Land
\r\n
\r\n\t
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\r\n\t5. Urban Planning and Environmental Management embracing SDG 7 on Affordable Clean Energy, SDG 9 on Industry, Innovation and Infrastructure, and SDG 11 on Sustainable Cities and Communities.
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\r\n\tThe series also seeks to support the use of cross cutting SDGs, as many of the goals listed above, targets and indicators are all interconnected to impact our lives and the decisions we make on a daily basis, making them impossible to tie to a single topic.
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His research interest focuses on computational chemistry and molecular modeling of diverse systems of pharmacological, food, and alternative energy interests by resorting to DFT and Conceptual DFT. He has authored a coauthored more than 255 peer-reviewed papers, 32 book chapters, and 2 edited books. He has delivered speeches at many international and domestic conferences. He serves as a reviewer for more than eighty international journals, books, and research proposals as well as an editor for special issues of renowned scientific journals.",institutionString:"Centro de Investigación en Materiales Avanzados",institution:{name:"Centro de Investigación en Materiales Avanzados",country:{name:"Mexico"}}},{id:"76477",title:"Prof.",name:"Mirza",middleName:null,surname:"Hasanuzzaman",slug:"mirza-hasanuzzaman",fullName:"Mirza Hasanuzzaman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/76477/images/system/76477.png",biography:"Dr. Mirza Hasanuzzaman is a Professor of Agronomy at Sher-e-Bangla Agricultural University, Bangladesh. He received his Ph.D. in Plant Stress Physiology and Antioxidant Metabolism from Ehime University, Japan, with a scholarship from the Japanese Government (MEXT). Later, he completed his postdoctoral research at the Center of Molecular Biosciences, University of the Ryukyus, Japan, as a recipient of the Japan Society for the Promotion of Science (JSPS) postdoctoral fellowship. He was also the recipient of the Australian Government Endeavour Research Fellowship for postdoctoral research as an adjunct senior researcher at the University of Tasmania, Australia. Dr. Hasanuzzaman’s current work is focused on the physiological and molecular mechanisms of environmental stress tolerance. Dr. Hasanuzzaman has published more than 150 articles in peer-reviewed journals. He has edited ten books and written more than forty book chapters on important aspects of plant physiology, plant stress tolerance, and crop production. According to Scopus, Dr. Hasanuzzaman’s publications have received more than 10,500 citations with an h-index of 53. He has been named a Highly Cited Researcher by Clarivate. He is an editor and reviewer for more than fifty peer-reviewed international journals and was a recipient of the “Publons Peer Review Award” in 2017, 2018, and 2019. He has been honored by different authorities for his outstanding performance in various fields like research and education, and he has received the World Academy of Science Young Scientist Award (2014) and the University Grants Commission (UGC) Award 2018. He is a fellow of the Bangladesh Academy of Sciences (BAS) and the Royal Society of Biology.",institutionString:"Sher-e-Bangla Agricultural University",institution:{name:"Sher-e-Bangla Agricultural University",country:{name:"Bangladesh"}}},{id:"187859",title:"Prof.",name:"Kusal",middleName:"K.",surname:"Das",slug:"kusal-das",fullName:"Kusal Das",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSBDeQAO/Profile_Picture_1623411145568",biography:"Kusal K. Das is a Distinguished Chair Professor of Physiology, Shri B. M. Patil Medical College and Director, Centre for Advanced Medical Research (CAMR), BLDE (Deemed to be University), Vijayapur, Karnataka, India. Dr. Das did his M.S. and Ph.D. in Human Physiology from the University of Calcutta, Kolkata. His area of research is focused on understanding of molecular mechanisms of heavy metal activated low oxygen sensing pathways in vascular pathophysiology. He has invented a new method of estimation of serum vitamin E. His expertise in critical experimental protocols on vascular functions in experimental animals was well documented by his quality of publications. He was a Visiting Professor of Medicine at University of Leeds, United Kingdom (2014-2016) and Tulane University, New Orleans, USA (2017). For his immense contribution in medical research Ministry of Science and Technology, Government of India conferred him 'G.P. Chatterjee Memorial Research Prize-2019” and he is also the recipient of 'Dr.Raja Ramanna State Scientist Award 2015” by Government of Karnataka. He is a Fellow of the Royal Society of Biology (FRSB), London and Honorary Fellow of Karnataka Science and Technology Academy, Department of Science and Technology, Government of Karnataka.",institutionString:"BLDE (Deemed to be University), India",institution:null},{id:"243660",title:"Dr.",name:"Mallanagouda Shivanagouda",middleName:null,surname:"Biradar",slug:"mallanagouda-shivanagouda-biradar",fullName:"Mallanagouda Shivanagouda Biradar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243660/images/system/243660.jpeg",biography:"M. S. Biradar is Vice Chancellor and Professor of Medicine of\nBLDE (Deemed to be University), Vijayapura, Karnataka, India.\nHe obtained his MD with a gold medal in General Medicine and\nhas devoted himself to medical teaching, research, and administrations. He has also immensely contributed to medical research\non vascular medicine, which is reflected by his numerous publications including books and book chapters. Professor Biradar was\nalso Visiting Professor at Tulane University School of Medicine, New Orleans, USA.",institutionString:"BLDE (Deemed to be University)",institution:{name:"BLDE University",country:{name:"India"}}},{id:"289796",title:"Dr.",name:"Swastika",middleName:null,surname:"Das",slug:"swastika-das",fullName:"Swastika Das",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/289796/images/system/289796.jpeg",biography:"Swastika N. Das is Professor of Chemistry at the V. P. Dr. P. G.\nHalakatti College of Engineering and Technology, BLDE (Deemed\nto be University), Vijayapura, Karnataka, India. She obtained an\nMSc, MPhil, and PhD in Chemistry from Sambalpur University,\nOdisha, India. Her areas of research interest are medicinal chemistry, chemical kinetics, and free radical chemistry. She is a member\nof the investigators who invented a new modified method of estimation of serum vitamin E. She has authored numerous publications including book\nchapters and is a mentor of doctoral curriculum at her university.",institutionString:"BLDEA’s V.P.Dr.P.G.Halakatti College of Engineering & Technology",institution:{name:"BLDE University",country:{name:"India"}}},{id:"248459",title:"Dr.",name:"Akikazu",middleName:null,surname:"Takada",slug:"akikazu-takada",fullName:"Akikazu Takada",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248459/images/system/248459.png",biography:"Akikazu Takada was born in Japan, 1935. After graduation from\nKeio University School of Medicine and finishing his post-graduate studies, he worked at Roswell Park Memorial Institute NY,\nUSA. He then took a professorship at Hamamatsu University\nSchool of Medicine. In thrombosis studies, he found the SK\npotentiator that enhances plasminogen activation by streptokinase. He is very much interested in simultaneous measurements\nof fatty acids, amino acids, and tryptophan degradation products. By using fatty\nacid analyses, he indicated that plasma levels of trans-fatty acids of old men were\nfar higher in the US than Japanese men. . He also showed that eicosapentaenoic acid\n(EPA) and docosahexaenoic acid (DHA) levels are higher, and arachidonic acid\nlevels are lower in Japanese than US people. By using simultaneous LC/MS analyses\nof plasma levels of tryptophan metabolites, he recently found that plasma levels of\nserotonin, kynurenine, or 5-HIAA were higher in patients of mono- and bipolar\ndepression, which are significantly different from observations reported before. In\nview of recent reports that plasma tryptophan metabolites are mainly produced by\nmicrobiota. He is now working on the relationships between microbiota and depression or autism.",institutionString:"Hamamatsu University School of Medicine",institution:{name:"Hamamatsu University School of Medicine",country:{name:"Japan"}}},{id:"137240",title:"Prof.",name:"Mohammed",middleName:null,surname:"Khalid",slug:"mohammed-khalid",fullName:"Mohammed Khalid",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/137240/images/system/137240.png",biography:"Mohammed Khalid received his B.S. degree in chemistry in 2000 and Ph.D. degree in physical chemistry in 2007 from the University of Khartoum, Sudan. He moved to School of Chemistry, Faculty of Science, University of Sydney, Australia in 2009 and joined Dr. Ron Clarke as a postdoctoral fellow where he worked on the interaction of ATP with the phosphoenzyme of the Na+/K+-ATPase and dual mechanisms of allosteric acceleration of the Na+/K+-ATPase by ATP; then he went back to Department of Chemistry, University of Khartoum as an assistant professor, and in 2014 he was promoted as an associate professor. In 2011, he joined the staff of Department of Chemistry at Taif University, Saudi Arabia, where he is currently an assistant professor. His research interests include the following: P-Type ATPase enzyme kinetics and mechanisms, kinetics and mechanisms of redox reactions, autocatalytic reactions, computational enzyme kinetics, allosteric acceleration of P-type ATPases by ATP, exploring of allosteric sites of ATPases, and interaction of ATP with ATPases located in cell membranes.",institutionString:"Taif University",institution:{name:"Taif University",country:{name:"Saudi Arabia"}}},{id:"63810",title:"Prof.",name:"Jorge",middleName:null,surname:"Morales-Montor",slug:"jorge-morales-montor",fullName:"Jorge Morales-Montor",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/63810/images/system/63810.png",biography:"Dr. Jorge Morales-Montor was recognized with the Lola and Igo Flisser PUIS Award for best graduate thesis at the national level in the field of parasitology. He received a fellowship from the Fogarty Foundation to perform postdoctoral research stay at the University of Georgia. He has 153 journal articles to his credit. He has also edited several books and published more than fifty-five book chapters. He is a member of the Mexican Academy of Sciences, Latin American Academy of Sciences, and the National Academy of Medicine. He has received more than thirty-five awards and has supervised numerous bachelor’s, master’s, and Ph.D. students. Dr. Morales-Montor is the past president of the Mexican Society of Parasitology.",institutionString:"National Autonomous University of Mexico",institution:{name:"National Autonomous University of Mexico",country:{name:"Mexico"}}},{id:"217215",title:"Dr.",name:"Palash",middleName:null,surname:"Mandal",slug:"palash-mandal",fullName:"Palash Mandal",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217215/images/system/217215.jpeg",biography:null,institutionString:"Charusat University",institution:null},{id:"49739",title:"Dr.",name:"Leszek",middleName:null,surname:"Szablewski",slug:"leszek-szablewski",fullName:"Leszek Szablewski",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49739/images/system/49739.jpg",biography:"Leszek Szablewski is a professor of medical sciences. He received his M.S. in the Faculty of Biology from the University of Warsaw and his PhD degree from the Institute of Experimental Biology Polish Academy of Sciences. He habilitated in the Medical University of Warsaw, and he obtained his degree of Professor from the President of Poland. Professor Szablewski is the Head of Chair and Department of General Biology and Parasitology, Medical University of Warsaw. Professor Szablewski has published over 80 peer-reviewed papers in journals such as Journal of Alzheimer’s Disease, Biochim. Biophys. Acta Reviews of Cancer, Biol. Chem., J. Biomed. Sci., and Diabetes/Metabol. Res. Rev, Endocrine. He is the author of two books and four book chapters. He has edited four books, written 15 scripts for students, is the ad hoc reviewer of over 30 peer-reviewed journals, and editorial member of peer-reviewed journals. Prof. Szablewski’s research focuses on cell physiology, genetics, and pathophysiology. He works on the damage caused by lack of glucose homeostasis and changes in the expression and/or function of glucose transporters due to various diseases. He has given lectures, seminars, and exercises for students at the Medical University.",institutionString:"Medical University of Warsaw",institution:{name:"Medical University of Warsaw",country:{name:"Poland"}}},{id:"173123",title:"Dr.",name:"Maitham",middleName:null,surname:"Khajah",slug:"maitham-khajah",fullName:"Maitham Khajah",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/173123/images/system/173123.jpeg",biography:"Dr. Maitham A. Khajah received his degree in Pharmacy from Faculty of Pharmacy, Kuwait University, in 2003 and obtained his PhD degree in December 2009 from the University of Calgary, Canada (Gastrointestinal Science and Immunology). Since January 2010 he has been assistant professor in Kuwait University, Faculty of Pharmacy, Department of Pharmacology and Therapeutics. His research interest are molecular targets for the treatment of inflammatory bowel disease (IBD) and the mechanisms responsible for immune cell chemotaxis. He cosupervised many students for the MSc Molecular Biology Program, College of Graduate Studies, Kuwait University. Ever since joining Kuwait University in 2010, he got various grants as PI and Co-I. He was awarded the Best Young Researcher Award by Kuwait University, Research Sector, for the Year 2013–2014. He was a member in the organizing committee for three conferences organized by Kuwait University, Faculty of Pharmacy, as cochair and a member in the scientific committee (the 3rd, 4th, and 5th Kuwait International Pharmacy Conference).",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"195136",title:"Dr.",name:"Aya",middleName:null,surname:"Adel",slug:"aya-adel",fullName:"Aya Adel",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/195136/images/system/195136.jpg",biography:"Dr. Adel works as an Assistant Lecturer in the unit of Phoniatrics, Department of Otolaryngology, Ain Shams University in Cairo, Egypt. Dr. Adel is especially interested in joint attention and its impairment in autism spectrum disorder",institutionString:"Ain Shams University",institution:{name:"Ain Shams University",country:{name:"Egypt"}}},{id:"94911",title:"Dr.",name:"Boulenouar",middleName:null,surname:"Mesraoua",slug:"boulenouar-mesraoua",fullName:"Boulenouar Mesraoua",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94911/images/system/94911.png",biography:"Dr Boulenouar Mesraoua is the Associate Professor of Clinical Neurology at Weill Cornell Medical College-Qatar and a Consultant Neurologist at Hamad Medical Corporation at the Neuroscience Department; He graduated as a Medical Doctor from the University of Oran, Algeria; he then moved to Belgium, the City of Liege, for a Residency in Internal Medicine and Neurology at Liege University; after getting the Belgian Board of Neurology (with high marks), he went to the National Hospital for Nervous Diseases, Queen Square, London, United Kingdom for a fellowship in Clinical Neurophysiology, under Pr Willison ; Dr Mesraoua had also further training in Epilepsy and Continuous EEG Monitoring for two years (from 2001-2003) in the Neurophysiology department of Zurich University, Switzerland, under late Pr Hans Gregor Wieser ,an internationally known epileptologist expert. \n\nDr B. Mesraoua is the Director of the Neurology Fellowship Program at the Neurology Section and an active member of the newly created Comprehensive Epilepsy Program at Hamad General Hospital, Doha, Qatar; he is also Assistant Director of the Residency Program at the Qatar Medical School. \nDr B. Mesraoua's main interests are Epilepsy, Multiple Sclerosis, and Clinical Neurology; He is the Chairman and the Organizer of the well known Qatar Epilepsy Symposium, he is running yearly for the past 14 years and which is considered a landmark in the Gulf region; He has also started last year , together with other epileptologists from Qatar, the region and elsewhere, a yearly International Epilepsy School Course, which was attended by many neurologists from the Area.\n\nInternationally, Dr Mesraoua is an active and elected member of the Commission on Eastern Mediterranean Region (EMR ) , a regional branch of the International League Against Epilepsy (ILAE), where he represents the Middle East and North Africa(MENA ) and where he holds the position of chief of the Epilepsy Epidemiology Section; Dr Mesraoua is a member of the American Academy of Neurology, the Europeen Academy of Neurology and the American Epilepsy Society.\n\nDr Mesraoua's main objectives are to encourage frequent gathering of the epileptologists/neurologists from the MENA region and the rest of the world, promote Epilepsy Teaching in the MENA Region, and encourage multicenter studies involving neurologists and epileptologists in the MENA region, particularly epilepsy epidemiological studies. \n\nDr. Mesraoua is the recipient of two research Grants, as the Lead Principal Investigator (750.000 USD and 250.000 USD) from the Qatar National Research Fund (QNRF) and the Hamad Hospital Internal Research Grant (IRGC), on the following topics : “Continuous EEG Monitoring in the ICU “ and on “Alpha-lactoalbumin , proof of concept in the treatment of epilepsy” .Dr Mesraoua is a reviewer for the journal \"seizures\" (Europeen Epilepsy Journal ) as well as dove journals ; Dr Mesraoua is the author and co-author of many peer reviewed publications and four book chapters in the field of Epilepsy and Clinical Neurology",institutionString:"Weill Cornell Medical College in Qatar",institution:{name:"Weill Cornell Medical College in Qatar",country:{name:"Qatar"}}},{id:"282429",title:"Prof.",name:"Covanis",middleName:null,surname:"Athanasios",slug:"covanis-athanasios",fullName:"Covanis Athanasios",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/282429/images/system/282429.jpg",biography:null,institutionString:"Neurology-Neurophysiology Department of the Children Hospital Agia Sophia",institution:null},{id:"190980",title:"Prof.",name:"Marwa",middleName:null,surname:"Mahmoud Saleh",slug:"marwa-mahmoud-saleh",fullName:"Marwa Mahmoud Saleh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/190980/images/system/190980.jpg",biography:"Professor Marwa Mahmoud Saleh is a doctor of medicine and currently works in the unit of Phoniatrics, Department of Otolaryngology, Ain Shams University in Cairo, Egypt. She got her doctoral degree in 1991 and her doctoral thesis was accomplished in the University of Iowa, United States. Her publications covered a multitude of topics as videokymography, cochlear implants, stuttering, and dysphagia. She has lectured Egyptian phonology for many years. Her recent research interest is joint attention in autism.",institutionString:"Ain Shams University",institution:{name:"Ain Shams University",country:{name:"Egypt"}}},{id:"259190",title:"Dr.",name:"Syed Ali Raza",middleName:null,surname:"Naqvi",slug:"syed-ali-raza-naqvi",fullName:"Syed Ali Raza Naqvi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259190/images/system/259190.png",biography:"Dr. Naqvi is a radioanalytical chemist and is working as an associate professor of analytical chemistry in the Department of Chemistry, Government College University, Faisalabad, Pakistan. Advance separation techniques, nuclear analytical techniques and radiopharmaceutical analysis are the main courses that he is teaching to graduate and post-graduate students. In the research area, he is focusing on the development of organic- and biomolecule-based radiopharmaceuticals for diagnosis and therapy of infectious and cancerous diseases. Under the supervision of Dr. Naqvi, three students have completed their Ph.D. degrees and 41 students have completed their MS degrees. He has completed three research projects and is currently working on 2 projects entitled “Radiolabeling of fluoroquinolone derivatives for the diagnosis of deep-seated bacterial infections” and “Radiolabeled minigastrin peptides for diagnosis and therapy of NETs”. He has published about 100 research articles in international reputed journals and 7 book chapters. Pakistan Institute of Nuclear Science & Technology (PINSTECH) Islamabad, Punjab Institute of Nuclear Medicine (PINM), Faisalabad and Institute of Nuclear Medicine and Radiology (INOR) Abbottabad are the main collaborating institutes.",institutionString:"Government College University",institution:{name:"Government College University, Faisalabad",country:{name:"Pakistan"}}},{id:"58390",title:"Dr.",name:"Gyula",middleName:null,surname:"Mozsik",slug:"gyula-mozsik",fullName:"Gyula Mozsik",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/58390/images/system/58390.png",biography:"Gyula Mózsik MD, Ph.D., ScD (med), is an emeritus professor of Medicine at the First Department of Medicine, Univesity of Pécs, Hungary. He was head of this department from 1993 to 2003. His specializations are medicine, gastroenterology, clinical pharmacology, clinical nutrition, and dietetics. His research fields are biochemical pharmacological examinations in the human gastrointestinal (GI) mucosa, mechanisms of retinoids, drugs, capsaicin-sensitive afferent nerves, and innovative pharmacological, pharmaceutical, and nutritional (dietary) research in humans. He has published about 360 peer-reviewed papers, 197 book chapters, 692 abstracts, 19 monographs, and has edited 37 books. He has given about 1120 regular and review lectures. He has organized thirty-eight national and international congresses and symposia. He is the founder of the International Conference on Ulcer Research (ICUR); International Union of Pharmacology, Gastrointestinal Section (IUPHAR-GI); Brain-Gut Society symposiums, and gastrointestinal cytoprotective symposiums. He received the Andre Robert Award from IUPHAR-GI in 2014. Fifteen of his students have been appointed as full professors in Egypt, Cuba, and Hungary.",institutionString:"University of Pécs",institution:{name:"University of Pecs",country:{name:"Hungary"}}},{id:"277367",title:"M.Sc.",name:"Daniel",middleName:"Martin",surname:"Márquez López",slug:"daniel-marquez-lopez",fullName:"Daniel Márquez López",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/277367/images/7909_n.jpg",biography:"Msc Daniel Martin Márquez López has a bachelor degree in Industrial Chemical Engineering, a Master of science degree in the same área and he is a PhD candidate for the Instituto Politécnico Nacional. His Works are realted to the Green chemistry field, biolubricants, biodiesel, transesterification reactions for biodiesel production and the manipulation of oils for therapeutic purposes.",institutionString:null,institution:{name:"Instituto Politécnico Nacional",country:{name:"Mexico"}}},{id:"196544",title:"Prof.",name:"Angel",middleName:null,surname:"Catala",slug:"angel-catala",fullName:"Angel Catala",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/196544/images/system/196544.jpg",biography:"Angel Catalá studied chemistry at Universidad Nacional de La Plata, Argentina, where he received a Ph.D. in Chemistry (Biological Branch) in 1965. From 1964 to 1974, he worked as an Assistant in Biochemistry at the School of Medicine at the same university. From 1974 to 1976, he was a fellow of the National Institutes of Health (NIH) at the University of Connecticut, Health Center, USA. From 1985 to 2004, he served as a Full Professor of Biochemistry at the Universidad Nacional de La Plata. He is a member of the National Research Council (CONICET), Argentina, and the Argentine Society for Biochemistry and Molecular Biology (SAIB). His laboratory has been interested for many years in the lipid peroxidation of biological membranes from various tissues and different species. Dr. Catalá has directed twelve doctoral theses, published more than 100 papers in peer-reviewed journals, several chapters in books, and edited twelve books. He received awards at the 40th International Conference Biochemistry of Lipids 1999 in Dijon, France. He is the winner of the Bimbo Pan-American Nutrition, Food Science and Technology Award 2006 and 2012, South America, Human Nutrition, Professional Category. In 2006, he won the Bernardo Houssay award in pharmacology, in recognition of his meritorious works of research. Dr. Catalá belongs to the editorial board of several journals including Journal of Lipids; International Review of Biophysical Chemistry; Frontiers in Membrane Physiology and Biophysics; World Journal of Experimental Medicine and Biochemistry Research International; World Journal of Biological Chemistry, Diabetes, and the Pancreas; International Journal of Chronic Diseases & Therapy; and International Journal of Nutrition. He is the co-editor of The Open Biology Journal and associate editor for Oxidative Medicine and Cellular Longevity.",institutionString:"Universidad Nacional de La Plata",institution:{name:"National University of La Plata",country:{name:"Argentina"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",slug:"francisco-javier-martin-romero",fullName:"Francisco Javier Martin-Romero",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",biography:"Francisco Javier Martín-Romero (Javier) is a Professor of Biochemistry and Molecular Biology at the University of Extremadura, Spain. He is also a group leader at the Biomarkers Institute of Molecular Pathology. Javier received his Ph.D. in 1998 in Biochemistry and Biophysics. At the National Cancer Institute (National Institute of Health, Bethesda, MD) he worked as a research associate on the molecular biology of selenium and its role in health and disease. After postdoctoral collaborations with Carlos Gutierrez-Merino (University of Extremadura, Spain) and Dario Alessi (University of Dundee, UK), he established his own laboratory in 2008. The interest of Javier's lab is the study of cell signaling with a special focus on Ca2+ signaling, and how Ca2+ transport modulates the cytoskeleton, migration, differentiation, cell death, etc. He is especially interested in the study of Ca2+ channels, and the role of STIM1 in the initiation of pathological events.",institutionString:null,institution:{name:"University of Extremadura",country:{name:"Spain"}}},{id:"217323",title:"Prof.",name:"Guang-Jer",middleName:null,surname:"Wu",slug:"guang-jer-wu",fullName:"Guang-Jer Wu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217323/images/8027_n.jpg",biography:null,institutionString:null,institution:null},{id:"148546",title:"Dr.",name:"Norma Francenia",middleName:null,surname:"Santos-Sánchez",slug:"norma-francenia-santos-sanchez",fullName:"Norma Francenia Santos-Sánchez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/148546/images/4640_n.jpg",biography:null,institutionString:null,institution:null},{id:"272889",title:"Dr.",name:"Narendra",middleName:null,surname:"Maddu",slug:"narendra-maddu",fullName:"Narendra Maddu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/272889/images/10758_n.jpg",biography:null,institutionString:null,institution:null},{id:"242491",title:"Prof.",name:"Angelica",middleName:null,surname:"Rueda",slug:"angelica-rueda",fullName:"Angelica Rueda",position:"Investigador Cinvestav 3B",profilePictureURL:"https://mts.intechopen.com/storage/users/242491/images/6765_n.jpg",biography:null,institutionString:null,institution:null},{id:"88631",title:"Dr.",name:"Ivan",middleName:null,surname:"Petyaev",slug:"ivan-petyaev",fullName:"Ivan Petyaev",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Lycotec (United Kingdom)",country:{name:"United Kingdom"}}},{id:"423869",title:"Ms.",name:"Smita",middleName:null,surname:"Rai",slug:"smita-rai",fullName:"Smita Rai",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Integral University",country:{name:"India"}}},{id:"424024",title:"Prof.",name:"Swati",middleName:null,surname:"Sharma",slug:"swati-sharma",fullName:"Swati Sharma",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Integral University",country:{name:"India"}}},{id:"439112",title:"MSc.",name:"Touseef",middleName:null,surname:"Fatima",slug:"touseef-fatima",fullName:"Touseef Fatima",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Integral University",country:{name:"India"}}},{id:"424836",title:"Dr.",name:"Orsolya",middleName:null,surname:"Borsai",slug:"orsolya-borsai",fullName:"Orsolya Borsai",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca",country:{name:"Romania"}}},{id:"422262",title:"Ph.D.",name:"Paola Andrea",middleName:null,surname:"Palmeros-Suárez",slug:"paola-andrea-palmeros-suarez",fullName:"Paola Andrea Palmeros-Suárez",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Guadalajara",country:{name:"Mexico"}}}]}},subseries:{item:{id:"17",type:"subseries",title:"Metabolism",keywords:"Biomolecules Metabolism, Energy Metabolism, Metabolic Pathways, Key Metabolic Enzymes, Metabolic Adaptation",scope:"Metabolism is frequently defined in biochemistry textbooks as the overall process that allows living systems to acquire and use the free energy they need for their vital functions or the chemical processes that occur within a living organism to maintain life. Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. Thus all studies on metabolism will be considered for publication.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11413,editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",slug:"yannis-karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",biography:"Yannis Karamanos, born in Greece in 1953, completed his pre-graduate studies at the Université Pierre et Marie Curie, Paris, then his Masters and Doctoral degree at the Université de Lille (1983). He was associate professor at the University of Limoges (1987) before becoming full professor of biochemistry at the Université d’Artois (1996). He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. 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