\r\n\tThe application of these complex statistical models provides a greater understanding of the complex processes and mechanisms. Complex statistical models are a powerful tool that enables researchers in the health and medical sciences to increase their knowledge and understanding of complex systems and thus advance knowledge.
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The aquafeed market is estimated to account for USD 50.6 billion in 2020 and with compound annual growth rate of 7.2%, it is projected to reach USD 71.6 billion by 2025 [1]. Two main factors amplify such a lucrative revenue in aquafeed market including increase in global seafood consumption (122% from 1990 to 2018) and fast grow rate in aquaculture production (527% from 1990 to 2018) [2]. In fact, the annual growth rate of aquaculture industry was about 10% during the 1990s and about 5.8% annually from 2000 to 2018 indicating aquaculture is the fastest growing food production sector in the world [3]. According to Food and Agriculture Organization of the United Nations [2] the aquaculture accounted for 52% (54.3 million tonnes (MT)) of global fish production that means this industry supplied 17% of total animal proteins for the global population [2]. About 70% of aquaculture production relied on the aquafeeds, which is the main expenditure (∼ 40%–70% of the total expenses) and the largest input in this industry [4, 5]. Thus, the development of the aquafeed industry along with the improvement of feed efficiency are prerequisite to achieve the projected aquaculture production. On the other hand, aquafeed production industries mainly depend on marine-derived ingredients namely fish meal (FM) and fish oil (FO), but increasing demands for these marine-derived feedstuffs along with overexploitation and/or static tendency in capture fisheries of small pelagic fish resulted in uncertain supply and the inflation of their prices that adversely affect the profitability margins of aquaculture [6, 7]. It has been reported that about 70% to 80% of all produced FM is used in aquafeed industry [3]. Thus, seeking out environmentally friendly and economic alternative ingredients for substitution of FM and FO in aquafeeds formulation is a fundamental goal for aquaculture sustainability [7].
An alternative feedstuff for FM should possess some properties such as high availability, commercial competitive cost as well as ease of shipping and storage [8]. In addition, by considering nutritional aspects, an alternative protein source (APS) should have low quantities of fiber (< 6%), carbohydrates including starch (< 20%) and non-starch polysaccharides (NSP)(< 8%), anti-nutritional factors (ANF), high digestible protein level (≥ 48%), appropriate essential amino acid (EAA) profile (arginine > 3, lysine > 3.5, methionine > 1.5, threonine > 2.2% of total AA profile) and suitable palatability [6, 8]. On the other hand, it has been predicted that the application of FM in aquafeeds will be dropped to under 10% in some most popular aquaculture species such as omnivorous (e.g. carp, catfish and tilapia, 1-2% FM) and carnivorous fish species (e.g. marine fish, salmon and trout, 5-10% FM) [9]. Such tremendous shift from FM to APS in aquafeeds has resulted in new challenges in performance, feed utilization, welfare, and final product quality of cultured aquatic animal species [6].
Among alternative plant protein sources (APPS), legumes are the most abundant protein rich ingredients for applying in aquafeeds [10]. However, these APPS have several drawbacks such as a wide range of protein contents, EAA imbalances or inadequacies (e.g. sulfur amino acids including cysteine, methionine and taurine), low bioavailability of some minerals or microelements as they bounded with phytic acid (e.g. phosphorous, iodine, calcium and selenium) and the presence of high amounts of various ANF (e.g. antitrypsin factors, phytates, saponins, and polyphenols), which consequently impose some challenges in their use in aquafeeds formulations [8, 11]. Although some processed protein derivatives of legumes such as soy protein concentrate, soy protein isolate or pea protein isolate contain high protein levels (65-90%) and have low ANF concentrations, but they are too expensive to be used in most aquafeeds. In addition, the agriculture industry also restricted to develop production of these APPS without putting extra stress on land, water, and phosphorous resources. In this chapter it was aimed to highlight the opportunities and challenges in application of legumes as APPS and providing some strategies for enhancing their efficiency in aquafeeds.
2. Legumes, alternative protein sources for aquafeeds
The family Fabaceae (Leguminosae) or commonly named legumes are the third largest family of flowering plants with almost 770 genera and over 19,500 species [12, 13]. The largest and the most important economically subfamily of the legumes, are the Faboideae, which are the source of primary crops including dry seeds (e.g. lentils, broad beans, beans and peas), flavoring plants (e.g. carob and lupins), fodder plants (e.g. alfalfa) and oilseeds (e.g. peanut or groundnut and soybeans). The seeds are the most important part of the legumes that used as ingredients for aquafeeds manufacturing. In this section, it has been tried to introduce the most important legumes used in the aquaculture industry. In 2020, the global production of some legumes such as peanut, dry pea, chick pea, cow pea, lentil and lupins were 48.757, 48.75, 14.184, 14.246, 8.786, 5.734 and 1.01 MT, respectively [14].
2.1 Soybean (Glycine max)
Soybean (Glycine max) is the leading oilseed crop in the world and occupies the first place as a global APS for FM due to its large amounts of protein (∼ 40%) and reasonable EAA profile as well as its most availability in the global market [8]. Moreover, owing to genetic engineering technologies and plant biotechnology available for the crop, the output traits of soybean also improved for aquaculture purposes [15]. The global production of soybean was estimated to be 336.7 MT in 2020 and projected to reach to 371.3 MT in 2030 with annual growth rate of 1.8% from 2020 to 2030. A huge amount of this crop used for oil extraction that yield a cake with high levels of protein that will be processed to a wide range of soy products including soy flour, soybean meal (SBM), full-fat SBM, soy protein concentrate (SPC), soy protein isolate (SPI), soy protein hydrolysate (SPH) and fermented SBM (FSBM).
2.2 Soybean meal
Unprocessed SBM contains 30-50% protein, about 30% carbohydrates including oligosaccharides (e.g. stachyose and raffinose), starch and NSP (e.g. cellulose, hemicellulose, and pectins; Table 1) [16, 17, 18]. Except for cystine, the amounts of the EAA (mainly lysine, methionine and threonine; Table 2), taurine and tyrosine in SBM are generally lower than FM [8]. The protein level and EAA content can be enhanced by chemical processing of soy flakes to SPC and SPI or through hydrolysation and fermentation of SBM. In addition, high levels of ANF in SBM can be reduced by conventional processing (e.g. heat, autoclaving, and use of solvents).
Protein sources | Bioactive component |
---|
Oxidized and polymerized lipids | Histamine and putrescine | Nitosamines | Protease/trypsin inhibitors | Amylase inhibitors | Saponins | Phytate | Glucosinolates | Sterols | Oxalate | Anti-vitamins | Polyphenols/Tannins | Lectins | Phytoestrogens | Alkaloids | Cyanogens | NSP | Oligosacharide | Antigenic proteins |
---|
Fish meal | ● | ● | ● | | | | | | | | | | | | | | | | |
Soybean | ● | | | ● | | ● | ● | ● | ● | | ● | | ● | ● | | | ● | ● | ● |
Faba bean | | | | ● | | ● | ● | | ● | | | ● | ● | | | | | | ● |
Lupin | | | | ● | | ● | ● | | ● | | | ● | | ● | ● | ● | ● | ● | |
Peas | | | | ● | ● | ● | ● | | ● | ● | ● | ● | ● | | | ● | | ● | |
Pea nut | | | | ● | ● | | ● | | ● | | | ● | | | ● | | | | |
Alfalfa | | | | | | ● | | | ● | | ● | ● | | ● | ● | | | | |
carob seed germ meal | | | | ● | | ● | | | ● | | | ● | | | | | | | |
Soy/Lupin/Pea/Faba/Alfalfa PC | | | | | | ● | ● | | ● | | | ● | | | | | | | |
Soy/Lupin/Pea PI | | | | | | ● | | | | | | | | | | | | | |
Novel varieties | | | | ● | ● | ● | ● | | ● | | ● | | | | | | ● | ● | ● |
Table 1.
Bioactive component present in legumes and means of alleviation.
PC: protein concentrate; PI: Protein Isolate.
Protein sources | Protein content(%) | Lys(CS) | Met(CS) | Cys | Arg(CS) | Trp | Leu(CS) | Ile(CS) | Phe | His | Thr(CS) | Tyr | Val | Arg/Lys(CS) | Met/Lys(CS) |
---|
g.100 g−1 protein |
---|
Fish meal | 70 | 7.5 | 2.7 | 0.8 | 6.2 | 0.6 | 7.2 | 4.2 | 3.8 | 2.4 | 4.1 | 2.7 | 4.9 | 0.83 | 0.36 |
Faba bean | 29.0 | 6.3 (16↓) | 0.8(70.4↓) | 1.2 | 9.0(45.2↑) | 0.3 | 7.1(1.4↓) | 4.1(2.4↓) | 4.0 | 2.6 | 3.5(14.6↓) | 1.2 | 4.6 | 1.43(72.3↑) | 0.13(64↓) |
Faba bean PC | 63 | 6.63(11.6↓) | 0.71(73.7↓) | 1.1 | 8.68(40↑) | 1 | 7.57( | 4.1(2.4↓) | 4.35 | 2.5 | 3.49(14.9↓) | 3.35 | 4.48 | 1.31(57.8↑) | 0.11(69.4↓) |
Lupin (Lupinnus angustifolius) | 33.8 | 4.7(37.3↓) | 0.7(74.1↓) | 1.5 | 11.0(77.4↑) | | 6.9(4.2↓) | 4.2(═) | 4.0 | 2.7 | 3.4(17.1↓) | 3.6 | 3.9 | 2.34(182↑) | 0.15(58.3↓) |
dehulled lupin (Lupinus albus) meal | 42 | 5(33.3↓) | 0.8(70.4↓) | 1.6 | 11.3(82.3↑) | | 7.3(1.4↑) | 4.2(═) | 3.9 | 2.3 | 3.8(7.3↓) | 4.8 | 4 | 2.26(172.3↑) | 0.16(55.6↓) |
Lupin PI | 61 | 3.44(54.1↓) | 0.6(77.8↓) | 0.33 | 9.02(45.5↑) | | 5.25(27.1↓) | 2.46(41.4↓) | 2.95 | 1.97 | 2.62(36.1↓) | 3.11 | 2.29 | 2.62(215.7↑) | 0.17(52.8↓) |
Fermented Lupin | 40 | 5.57(25.7↓) | 0.82(69.6↓) | 1.4 | 9.75(57.3↑) | 0.83 | 7.3(1.4↑) | 4.6(9.5↑) | 4.25 | 3.3 | 4.1(═) | 4.25 | 4.75 | 1.75(110.8↑) | 0.15(58.3↓) |
Pea seed | 23.9 | 7.2(4↓) | 1.0(63↓) | 1.4 | 8.4(35.5↑) | | 7.1(1.4↓) | 4.2(═) | 4.7 | 2.5 | 3.8(7.3↓) | 3.1 | 4.8 | 1.17(41↑) | 0.14(61.1↓) |
Filed pea PC | 46.5 | 7.61(1.5↑) | 0.9(66.7↓) | 1.25 | 8.32(34.2↑) | 0.99 | 7.31(1.5↓) | 4.26(1.4↑) | 4.95 | 2.39 | 3.51(14.4↓) | 3.31 | 4.73 | 1.1(32.5↑) | 0.12(66.7↓) |
Pea PI | 80 | 6(20↓) | 0.78(71.1↓) | 0.25 | 7.4(19.4↑) | | 7.13(1↓) | 3(28.6↓) | 4.75 | 2 | 3.13(23.7↓) | 3.25 | 3.5 | 1.23(48↑) | 0.78(116.7↑) |
Bambara nut | | 8(6.7↑) | 0.64(76.3↓) | 2.41 | 7.48(20.6↑) | 0.6 | 10.2(41.7↑) | 5.45(29.6↑) | 7.69 | 3.86 | 4.43(8↑) | 3.13 | 6.24 | 0.94(13↑) | 0.08(77.8↓) |
Full fat soy bean meal | 36 | 6.3(16↓) | 1.29(52.2↓) | | 7.44(20↑) | 1.44 | 7.08(1.7↓) | 5.31(26.4↑) | 5.2 | 2.58 | 4.18(2↑) | | 4.97 | 1.18(42↑) | 0.20(44.4↓) |
Soy bean (expeller) | 43.5–49.3 | 6.3(16↓) | 1.4(48.2↓) | 1.6 | 7.5(21↑) | 1.2 | 7.7(6.9↑) | 4.6(9.5↑) | 5.1 | 2.7 | 3.7(9.8↓) | 3.5 | 4.5 | 1.19(43.4↑) | 0.22(38.9↓) |
Soybean (dehulled) | 53.5 | 6.3(16↓) | 1.4(48.2↓) | 1.6 | 7.3(17.7↑) | 1.4 | 7.7(6.9↑) | 4.6(9.5↑) | 5.1 | 2.7 | 3.8(7.3↓) | 3.5 | 4.8 | 1.16(39.8↑) | 0.22(39.8↓) |
Soy PC | 67–72 | 6.3(16↓) | 1.3(51.9↓) | 1.25 | 7.3(17.7↑) | 1.5 | 7.9(9.7↑) | 4.6(9.5↑) | 5.1 | 2.6 | 4.3(4.9↑) | 3.5 | 4.8 | 1.16(39.8↑) | 0.21(41.7↓) |
Soy PI | 90–92 | 6.0(20↓) | 1.0(63↓) | | 7.2(16.1↑) | 1.2 | 7.8(8.3↑) | 4.5(7.1↑) | 5.2 | 2.5 | 3.5(14.6↓) | | 4.6 | 1.2(44.6↑) | 0.17(52.8↓) |
Fermented soy bean | 48.9 | 6.2(17.3↓) | 1.6(40.74↓) | | 7.8(25.8↑) | 1.23 | 8.2(13.9↑) | 4.91(16.9↑) | 5.5 | 2.66 | 4.1(═) | | 4.1 | 1.3(56.6↑) | 0.26(37.8↓) |
Pea nut meal (corticated-decorticated) | 32–46.5 | 2.29(69.5↓) | 0.53(80.4↓) | | 5.28(14.8↓) | 0.48 | 3.18(55.8↓) | 1.80(57↓) | 2.37 | 1.26 | 1.40(65.9↓) | | 2.08 | 2.3(177↑) | 0.23(36.1↓) |
Pea nut meal expeller (corticated-decorticated) | 34.1–46.5 | 2.46(67.2↓) | 0.43(84.1↓) | | 5.01(19.2↓) | 0.37 | 3.03(57.9↓) | 1.58(62.4↓) | 2.32 | 1.05 | 1.27(69↓) | | 2.06 | 3.43(313↑) | 0.29(19.4↓) |
(Chloroplastic) alfalfa leaf PC | 53.22 | 4.57(39.1↓) | 0.3(88.9↓) | | 5(19.3↓) | 4 | 8(11.1↑) | 4.57(8.8↑) | 5.2 | 2.4 | 4.5(9.8↑) | | 5.2 | 1.1(32.5↑) | 0.07(80.6↓) |
Cytoplasmic alfalfa leaf PC | 69.24 | 5.3(29.3↓) | 0.35(87↓) | | 5.8(6.4↓) | 4 | 9(25↑) | 5.25(25↑) | 5.88 | 2.97 | 5.2(26.8↑) | | 5.8 | 1.1(32.5↑) | 0.07(80.6↓) |
carob seed germ meal | 34.8 | 6.49(13.5↓) | 1.41(47.8↓) | | 14.1(127↑) | | 7.1 | 3.88(7.6↓) | 3.64 | 3.5 | 4.14(1↑) | | 4.31 | 2.17(161.4↑) | 0.22(38.9↓) |
Table 2.
Amino acid content of legumes products in comparison to fish meal.
PC: protein concentrate; PI: Protein Isolate; Lys: lysine; Met: methionine; Cys: cysteine; Arg: arginine; Trp: tryptophan; Leu: leucine; Ile: Isoleucine; Phe: phenylalanine; His: histidine; Thr: threonine; Tyr: tyrosine; Val: valine.
2.3 Soy protein concentrate
Soy protein concentrate is commonly obtained by fractionation of SBM through aqueous alcohol that not only enhance protein content (∼ 70%) [19, 20, 21] by extracting carbohydrates but also remove ANF (e.g. saponin), soluble oligosaccharides and fiber [21, 22, 23] that eventually increase its palatability and protein digestibility [24]. Soy protein concentrate has been getting more attention in aquafeed industry because of its well-balanced AA profile compared to other APPS [23, 25].
2.4 Soy protein isolate
Soy protein isolate is produced by refinement of SBM through a series of aqueous extractions with different pH levels that increase its protein content over 78% [26]. As, the processing of SPI does not have the aqueous alcohol extraction step, the saponin content of SPI (∼0.8%) is higher than that in SPC (∼0%) [27, 28]. Because of high protein content in SPI, the inclusion levels of this ingredient in aquafeeds could be lower than SBM and SPC that eventually reduce the total ANF input in aquafeed. On the other hand, it has been confirmed that the nutrients digestibility of SPI was higher than that of SPC, when it was replaced by 40% of FM in feed for rainbow trout (Oncorhynchus mykiss) [29].
2.5 Soy protein hydrolysates
Soy protein hydrolysate is produced by restricted enzymatic hydrolysis of soy products that consequently improves their nutritional and practical characteristics [30]. The enzymes generally use for hydrolysis of soy protein are mainly endo and exopeptidases (e.g. leucine aminopeptidase) that derived from fermentation of selected strains of bacteria (e.g. Bacillus licheniformis) [30]. The most important characteristics of SPH are the maximized digestibility of protein, excellent protein solubility and minimized ANF that ultimately enhance its efficiency. Significant solubility of the SPH is mainly due to the formation of short chain hydrophilic polypeptides and the elimination of insoluble fractions through a sedimentation step by a centrifugation process [31]. In addition, SPH has numerous bioactive low molecular weights peptides with health improving properties such as antioxidative and immunostimulatory compounds [32, 33, 34].
2.6 Fermented soybean meal
The fermentation process in aquaculture usually uses for enhancing the nutritional value and reducing ANF in alternative protein sources for incorporating into aquafeeds [35]. Fermentation of SBM by yeast (e.g. Saccharomyces cerevisiae), fungus (e.g. Aspergillus niger) or bacterial strains (e.g. Bacillus spp., L. plantarum P8, Pediococcus acidilacticstrains) can improve its nutritional quality and digestibility by providing low molecular weight peptides, increasing bioavailability of minerals and reducing its ANF (e.g. trypsin inhibitors) [35, 36, 37]. Moreover, fermented SBM has more protein content (∼10%) than SBM with negligible change of its EAA profile [37]. In addition, fermented SBM provide probiotic characteristics and can increase efficiency of aquafeeds by elevating trypsin and fibrinolytic enzymes activities [35].
2.7 Transgenic soybean
“Genetically modified plants are typically created by the addition or deletion of existing innate genes in the plant’s own genome or transferring external non-host genome through DNA splicing” [38]. Genetic approaches were applied for creating a prototype soybean that synthetize and accumulate a n-3 long chain polyunsaturated fatty acid (i.e. eicosapentaenoic acid, EPA) and a carotenoid (i.e. astaxanthin) in the seed [15]. Soybean contains very low levels of lutein (10 μg/g seed), but the expression of the phytoene synthase gene in transgenic soybean increases the accumulation of ß-carotene up to 800 μg/g seed and significantly reduces lutein content (∼29%). The expression of fatty acid elongases and Δ5 desaturase in transgenic soybean increase the synthesis of EPA up to 5%, but EPA content need to be improve in transgenic soybean to better reflect FO fatty acid profile [15]. It should be mentioned that the annual sales of astaxanthin reaches to over USD 200 million and inclusion of such trait in transgenic soybean can impressively enhance the attractiveness of such product especially for incorporating in aquafeeds for salmon, trout and shrimp [39, 40].
2.8 Pea protein
Pea (Pisum sativum) is another promising APPS for aquaculture species with highly digestible protein and energy levels and it is a good source of digestible starch (∼40-50%) [41, 42]. This legume contains low levels of ANF (e.g. tannins) and does not have trypsin inhibitors, but contain high levels of saponins. The protein (∼21-25%) and methionine contents in peas also lower compared to soybean. Pea protein concentrate (PPC) is a pea derived product that produced by fine grinding dehulled peas and air processing to remove fiber and carbohydrates. The PPC contains higher protein and lower ANF compared to unprocessed pea meal, thus it is more suitable APPS for aquafeeds. Like other plant protein derived products, extrusion and micronizing processes improve protein and energy digestibility of pea meals [41].
2.9 Peanut
Peanut (Arachis hypogaea) is the fourth largest oilseed crop in the world and peanut pulp that remain after the oil extraction can be used as an APPS in aquafeeds [43, 44]. Peanut meal (PNM) is a residue after solvent extraction of whole shelled peanuts and considered as a great APPS due to its higher protein content (∼47.8%) than SBM, higher palatability and the same cost as SBM [45, 46]. However, the protein quality of PNM is inferior compared to SBM and it contains lower levels of lysine and methionine than SBM, but a higher level of arginine [43, 45]. Lysine and methionine deficiencies in PNM can be met by using crystalline amino acids.
2.10 Lupines
Lupines include many legumes species with a considerable protein (∼ 35%) but low lipid (∼8–10%) levels [21, 47]. About 80% of lupines species the can be used as feed ingredients, particularly Lupinus angustifolius, is produced in Australia. Among different lupines, Andean lupin (L. mutabilis) seed contain ∼50% protein (dry matter) and its derivatives such as dehulled, deoiled and lupine protein concentrate contain higher protein content (∼61%) [48]. Although lupines have low levels of lysine and sulfur amino acids, they contain more arginine content than soybean [49]. Four species of lupines including L. angustifolius, L. albus, L. luteus and L. mutabilis named as “sweet lupins” as they contains low levels of alkaloids and because of their high protein contents they have great potential as APPS [50]. However, using lupins in aquafeeds are still limited because of their low protein digestibility and the presence of various ANF [51].
2.11 Faba bean
Faba bean, (Vicia faba L.) is a legume with high amount of protein (∼ 20 to 41%), carbohydrate (∼51% to 68%), B-vitamins and minerals depending on its variety [52, 53]. Its protein composition is mainly consisted of albumins (20%) and globulins (80%) and rich in glutamic and aspartic acids. But, the levels of sulfur amino acids and tryptophan residues are low [54]. The main carbohydrates in faba been are starch (∼41–53%), low molecular weight carbohydrates (e.g. raffinose , stachyose, and verbascose), and fiber mainly hemicellulose [52, 55]. Faba bean contains some ANF such as trypsin inhibitors and lectins, condensed tannin, phytic acid, vicine and convicine [56]. Processing of faba bean protein does provide ingredients with higher protein contents such as faba bean protein concentrate (∼55% crude protein) and faba bean isolate (∼80% crude protein) that contain lower levels of ANF [57, 58, 59].
2.12 Other protein sources
Carob seed (Ceratonia siliqua) germ does have a high protein content (∼45–50% crude protein) and it is cheaper than SBM [60]. Carob seed germ meal is produced from the germ of the carob seed after the separation of the gums and the fibrous [61, 62, 63]. However, it contains high levels of tannins [64].
Alfalfa (Medicago sativa) protein concentrate is another APPS that produced by pressing fresh alfalfa foliage (mainly leaves and stems) to make a protein-rich juice which is centrifuged and heated to fractionate proteins from the juice [65]. This byproduct contains reasonable protein level (∼52% crude protein) with high amounts of lysine, threonine, and methionine. It also contains high levels of vitamins and antioxidants such as carotenoids, but low content of fiber and ANF (e.g. phytic acid or lectins) [65, 66].
3. Anti-nutritional factors in legumes
The ANF are defined as compounds that disturb feed utilization and can affect the health condition and production of livestock [67]. Legumes contain various ANF such as saponins, tannins, phytic acid, gossypol, lectins, protease inhibitors, amylase inhibitors, antivitamin factors, metal binding ingredients, goitrogens, etc. (Table 1) that combine with nutrients and reduce bioavailability of them in aquafeeds [8]. Some ANF such as protease inhibitors and phytates abate digestibility of proteins and energy as well as reduce mineral absorption that consequently results in malnutrition and microelements deficiencies.
The ANF can be divided into four classes [66]:
Substances that affect dietary protein utilization (e.g. protease inhibitors, tannins and lectins).
Substances that influence dietary mineral utilization (e.g. phytates, gossypol, oxalates and glucosinolates)
Antivitamins
Miscellaneous (e.g. non-starch polysaccharides, mycotoxins, alkaloids, pyrimidine glycosides, phytoestrogens and saponins).
4. Improvement of legumes efficiency in aquafeeds
Digestibility of an ingredient is a pivotal parameter for determining its potential for use in the aquafeeds [68]. In order to validate the nutritional quality of a feedstuff, determination of apparent digestibility coefficient (ADC) of its dry matter and nutrients is necessary. As previously mentioned, generally legumes contain high amounts of starch and NSP and ANF [66] that negatively affect ADC in most fish and shrimp species. Carnivorous fish species are more susceptible to legumes. The ADC of crude protein of legumes are generally over 0.80, indicating high quality of protein provided by these APS. However, the ADC of gross energy in these research showed great fluctuations from 0.5 to 0.7 [69]. It has been reported that the ADC of legumes in diet mainly depends on fish species. Thus, ADC of legumes in omnivorous species such as Nile tilapia is higher than carnivorous fish such as rainbow trout [69].
Several strategies were applied for improving nutrients digestibility in legumes such as processing techniques (e.g. dehulling, soaking, extrusion cooking, fermentation etc.), using novel and new variety of plant protein sources (e.g. transgenic legumes), nutritional programming and selective breeding of fish to be more adapted to legumes in aquafeeds, modulation of gut microbiota (e.g. probiotics and short chain fatty acids), and inclusion of additives (e.g. acidifiers, CAA, phospholipids etc.) in aquafeeds [53, 70]. Here the most efficient strategies for reducing ANF in APPS were described:
4.1 Conventional strategies
Several physical processing strategies applied for removing, inactivating or reducing ANF (e.g. trypsin inhibitors, glucosinolates, tannins and saponins) contents in APPS including heat and/or soaking in water, dehulling and germination, roasting or autoclaving as well as extrusion and micronizing (infrared heat) [71] (Table 3). These conventional methods positively improve digestibility of legumes; however, these strategies are not conclusive in eradicating the adverse influences of ANF in legumes [72]. Moreover, some strategies such as heat damage lead to loss of some amino acids and adversely affect quality of proteins and carbohydrates through Malliard reactions [73, 74]. Furthermore, soaking in water may result in leaching water-soluble nutrients by this process.
Reduction strategies | Bioactive component |
---|
Protease/trypsin inhibitors | Amylase inhibitors | Saponins | Phytate | Glucosinolates | Oxalate | Anti-vitamins | Polyphenols/Tannins | Sterols | Lectins | Phytoestrogens | Alkaloids | Cyanogens | NSP | Oligosacharide | Antigenic proteins |
---|
Milling | | | | ● | | | | ● | | ● | | | | | | |
De-hulling | | | | | | | | ● | | | | ● | | | | |
Soaking | ● | | | ● | | | | ● | | | ● | ● | | | | ● |
Heat/Autoclaved | ● | ● | ● | ● | ● | ● | ● | ● | | ● | ● | | | | | ● |
Solvent extraction | | | ● | ● | | | ● | | ● | | ● | ● | | | ● | ● |
Germination | ● | | | ● | | | | ● | | | | | | | | ● |
Acid addition | ● | | ● | ● | | | ● | | | | | | | ● | ● | |
Alkalin addition | | | | | | | ● | ● | | | | ● | | ● | ● | ● |
enzymes addition | ● | ● | | ● | | | | | | | | | | ● | | ● |
Fermentation | ● | | ● | ● | | ● | | ● | | | ● | ● | ● | ● | ● | ● |
Micronutrient addition | ● | | | ● | | | | | ● | ● | | | ● | | | |
New Variates | | | | ● | ● | | | | | | | ● | | | | |
Table 3.
Legume bioactive compounds neutralizing strategies.
4.2 Exogenous enzymes and phytase
It has been confirmed that inclusion of carbohydrase exogenous enzymes such as xylanae, ß-glucanase and cellulase as well as phytase in aquafeeds can reduce the negative effects of NSP and phytate on digestion [75, 76]. Exogenous carbohydrases by facilitating carbohydrate digestion and reducing feed polymerization degree is going to decrease its viscosity and liberate carbohydrate oligomers [77]. In addition, carbohydrases by neutralizing NSP can increase the digestibility of energy, macronutrients and bioavailability of minerals because NSP reduce accessibility of enzymes to substrates and there is a relationship between phytate and NSP in PPS [75, 78]. In addition, carbohydrases may improve host’s gut health by supporting the propagation of beneficial microbiota in the gut that can facilitate fermentation of NSP and consequently increase the amounts of organic acids and especially short chain fatty acids production [78, 79].
4.3 Acidifiers
A plethora of studies confirmed that high amounts of dietary FM could be substituted with APPS by supplementing diet with short-chain fatty acids and acidifiers [80, 81]. In fact, acidification of plant protein based aquafeeds with acidifiers increase the bioavailability of minerals and trace elements and they neutralize or alleviate the negative impacts of ANF on nutrients digestibility [60, 82, 83, 84]. In addition, acidifiers by reducing the chyme pH through the gut can induce the pepsin activity [85]. Moreover, reduction of the chyme pH triggers the release of gastrointestinal hormones (e.g. secretin and cholecystokinin) that stimulate secretion of pancreatic digestive enzymes, which in turn elevates the digestibility of protein and minerals. Furthermore, it has been reported that acidifiers by controlling the appetite through the parasympathetic nerve system including orexigenic neurotransmitters that increase feed efficiency [86].
4.4 Gut microbiota as ANF biodegrading agent in fish
The application of the gut “indigenous” microbiota as probiotics can improve feed digestibility by supplying exogenous enzymes (e.g. cellulase, phytase, tannase and xylanases) and by eradicating and/or reducing ANF of the plant protein ingredients in the fish gut [87]. A plethora of studies have recognized cellulase-producing bacteria such as Citrobacter sp. C. freundii, Enterobacter sp. Bacillus coagulans, B. cereus, B. subtilis P6, B. velesensis P11, B. pumilus, B. tequilensis (KF640219), B. megaterium (KF640220) and B. altitudinis from the gut of various cultured fish species including Chinese carps [88, 89], Indian major carps [90, 91], tilapia (Oreochromis mossambica) [92], bata fish [93], murrels (Channa punctatus) [94], pacu (Piaractus mesopotamicus) and piaucom-pinta (Leporinus friderici) [95]. Using the above mentioned microorganisms as potential probiotics in aquafeeds or applying these microorganisms for fermentation of plant protein ingredients can provide great potential for eradicating ANF and improving their nutritional quality by boosting up EAA, minerals and vitamins bioavailability and increasing digestibility of protein and energy.
4.5 Other functional feed additives
Supplementing PP-based aquafeeds with additives can improve the digestibility of feed’s nutrients. In this context, it has been reported that supplementation of a diet contained high levels of legumes including SPC and pea protein (32%) with phosphatidylcholine pronouncedly improved lipid digestibility in Atlantic salmon [96].
On the other hand, it has been confirmed that the replacement of FM with PP sources could reduce cholesterol content in aquafeeds that may disturb bile acids synthesis in fish and result in low digestibility of lipid [97]. In this regard, it has been reported that supplementing SBM-based diets with cholesterol remarkably improved growth in channel catfish [98], turbot (Scophthalmus maximus) [85] and rainbow trout [99].
As mentioned earlier legumes are deficient in taurine or its precursors (i.e. cysteine and methionine) and some aquatic animal species especially marine fish unable, or have low ability to synthesize taurine [100]. Taurine is the main component of bile acids and increase the bile-salt dependent lipase activity in fish [101]. It has been proved that supplementation of soy protein-based aquafeeds with taurine improved growth performance, lipid metabolism, palatability, digestibility and overall nutritional quality of feeds in marine fish species such as common dentex (Dentex dentex) [101] and European sea bass larvae [102] and juveniles [103, 104].
Moreover, it has been confirmed that replacement of FM with plant protein sources with high levels of ANF (i.e. saponins, oligosaccharides, fibers and high molecular weight proteins) disturb bile metabolism in fish and may adversely affect fish productivity [105]. Bile acids as an emulsifier enhance digestion and absorption of lipid and lipid soluble nutrients through emulsification of lipids and activation of bile salt dependent lipase [105]. It also facilitates the excretion of cholesterol and toxic metabolites. The ANF in PP sources may induce gut inflammation that reduce resorption of bile acids or they may bind with bile salts and trigger extra excretion of bile acids into gut [105]. Thus, supplementing legume protein-based diet with bile acids may improve their efficiency and alleviate their negative effects on fish performance. For example, supplementing SBM-based diet with 1.5% bovine bile salts significantly improved growth rate in rainbow trout [106].
4.6 Nutritional programming and selective breeding
In recent years some studies were carried out on early nutritional programming of fish for increasing the acceptance of their offspring to new ingredients in aquafeeds. For instance, it has been reported that substitution of dietary FM and FO with vegetal feedstuffs through nutritional programming in brooders elevated the acceptance of vegetal ingredients and PP-based diets in rainbow trout [107] and gilthead seabream [108] offspring. In this regard, it has been reported that early nutritional programming in Atlantic salmon with a plant-based aquafeed enhanced growth rate and feed efficiency for 24% and 23%, respectively compared to those fed a diet contained FM and FO and then challenged with a plant-based aquafeed [109].
Recently, a new strain of rainbow trout (ARS-KO) was created by the US department of Agriculture by selective breeding over the course of two decades and this strain can grow better when fed with soy protein-based diets and does not develop enteritis [110, 111]. More research are required to be carried out in these genetic engineering to these novel techniques be advantageous and applicable at commercial stage.
5. Conclusions
Over the course of the past four decades, a great amount of knowledge has been gained in application of legumes as APS in aquafeeds, leading to a better comprehension regarding the impacts of these APPS on overall performance of different cultured species. Herbivorous and omnivorous fish and crustacean species have a great potential in utilization of legumes in their diets. Moreover, carnivorous species have mostly adapted to legumes-protein rich aquafeeds. However, in order to enhance the efficiency of legumes-protein based aquafeeds for carnivorous fish, further innovations and development is required by considering the cultured animal species and feed ingredients for increasing adaptability of cultured aquatic species to legumes. These innovations can be carried out in different aspects such as use of novel feedstuffs, eradication and/or reducing ANF, application of feed additives and use of precise feed formulations. The application of genetic engineering in legumes could result in the production of strains with low levels of ANF and make them appropriate for legumes-protein based aquafeeds. In addition, supplementing aquafeeds with functional feed additives can improve the efficiency of legumes for aquaculture nutrition. Using nutritional programming and applying genetic engineering also other novel strategies to provide new fish and crustacean strains with high capacity in acceptance and utilization of legumes in aquafeeds. Further studies are required to increase the efficiency of legumes in aquafeeds to support growth, health and welfare of cultured species.
\n',keywords:"additives, anti-nutritional factors, aquaculture, nutrients digestibility, essential amino acids",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/79107.pdf",chapterXML:"https://mts.intechopen.com/source/xml/79107.xml",downloadPdfUrl:"/chapter/pdf-download/79107",previewPdfUrl:"/chapter/pdf-preview/79107",totalDownloads:141,totalViews:0,totalCrossrefCites:0,dateSubmitted:"June 17th 2021",dateReviewed:"August 3rd 2021",datePrePublished:"October 25th 2021",datePublished:null,dateFinished:"October 25th 2021",readingETA:"0",abstract:"Aquaculture produce a great portion of aquatic derived proteins for human in the world. It has the highest and the fastest growth rate among the protein producing industries. Fish meal (FM) is the main and the most expensive ingredient for aquafeeds production. It provides protein, essential amino acids, energy, minerals and vitamins in aquafeeds. Given the current rapid development of aquaculture industry the competition for limited global supplies of FM may reduce its availability and elevate its price. Thus, finding high quality, economic and environmentally friendly alternative protein sources (APS) for aquafeeds production is vital for sustainability of the aquaculture industry. Among various APS, legumes have been proved to be promising APS because they have medium protein content with suitable amino acid profile, high digestible protein and energy levels, and appropriate minerals and vitamins for the most cultured aquatic species. They also are cost-effective and highly accessible. However, they contain various anti-nutritional factors that may reduce feed palatability and may negatively affect growth and health of cultured aquatic animal species. This chapter provide information regarding legumes and their derivatives as APS, their nutritional quality and their potential drawbacks. In addition, strategies for increasing the efficiency of legumes in aquafeeds are reviewed and discussed.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/79107",risUrl:"/chapter/ris/79107",signatures:"Fateme Hekmatpour and Mansour Torfi Mozanzadeh",book:{id:"12236",type:"book",title:"Legumes - Volume 2",subtitle:null,fullTitle:"Legumes - Volume 2",slug:null,publishedDate:null,bookSignature:"Dr. Jose Carlos Jimenez-Lopez and Dr. Alfonso Clemente",coverURL:"https://cdn.intechopen.com/books/images_new/12236.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-80356-915-4",printIsbn:"978-1-80356-914-7",pdfIsbn:"978-1-80356-916-1",isAvailableForWebshopOrdering:!0,editors:[{id:"33993",title:"Dr.",name:"Jose Carlos",middleName:null,surname:"Jimenez-Lopez",slug:"jose-carlos-jimenez-lopez",fullName:"Jose Carlos Jimenez-Lopez"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Legumes, alternative protein sources for aquafeeds",level:"1"},{id:"sec_2_2",title:"2.1 Soybean (Glycine max)",level:"2"},{id:"sec_3_2",title:"2.2 Soybean meal",level:"2"},{id:"sec_4_2",title:"2.3 Soy protein concentrate",level:"2"},{id:"sec_5_2",title:"2.4 Soy protein isolate",level:"2"},{id:"sec_6_2",title:"2.5 Soy protein hydrolysates",level:"2"},{id:"sec_7_2",title:"2.6 Fermented soybean meal",level:"2"},{id:"sec_8_2",title:"2.7 Transgenic soybean",level:"2"},{id:"sec_9_2",title:"2.8 Pea protein",level:"2"},{id:"sec_10_2",title:"2.9 Peanut",level:"2"},{id:"sec_11_2",title:"2.10 Lupines",level:"2"},{id:"sec_12_2",title:"2.11 Faba bean",level:"2"},{id:"sec_13_2",title:"2.12 Other protein sources",level:"2"},{id:"sec_15",title:"3. Anti-nutritional factors in legumes",level:"1"},{id:"sec_16",title:"4. Improvement of legumes efficiency in aquafeeds",level:"1"},{id:"sec_16_2",title:"4.1 Conventional strategies",level:"2"},{id:"sec_17_2",title:"4.2 Exogenous enzymes and phytase",level:"2"},{id:"sec_18_2",title:"4.3 Acidifiers",level:"2"},{id:"sec_19_2",title:"4.4 Gut microbiota as ANF biodegrading agent in fish",level:"2"},{id:"sec_20_2",title:"4.5 Other functional feed additives",level:"2"},{id:"sec_21_2",title:"4.6 Nutritional programming and selective breeding",level:"2"},{id:"sec_23",title:"5. Conclusions",level:"1"}],chapterReferences:[{id:"B1",body:'[Markets, Markets . https://wwwmarketsandmarketscom/new-reportshtml. 2021.]'},{id:"B2",body:'[FAO. Sustainability in action. State of World Fisheries and Aquaculture Rome. 2020;200.]'},{id:"B3",body:'[FAO. The State of World Fisheries and Aquaculture 2018—Meeting the sustainable development goals. Rome. Licence: CC BY-NC-SA 3.0 IGO. 2018.]'},{id:"B4",body:'[Henry M, Gasco L, Piccolo G, Fountoulaki E. 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DOI: 10.1111/are.13847.]'},{id:"B100",body:'[Reyshari A, Mohammadiazarm H, Mohammadian T, Torfi Mozanzadeh M. Effects of sodium diformate on growth performance, gut microflora, digestive enzymes and innate immunological parameters of Asian sea bass (Lates calcarifer) juveniles. Aquaculture Nutrition. 2019;25(5):1135-44. DOI: 10.1111/anu.12929.]'},{id:"B101",body:'[Sarker SA, Satoh S, Kiron V. Supplementation of citric acid and amino acid-chelated trace element to develop environment-friendly feed for red sea bream, Pagrus major. Aquaculture. 2005;248(1-4):3-11. DOI: 10.1016/j.aquaculture.2005.04.012.]'},{id:"B102",body:'[Liu Y, Chen Z, Dai J, Yang P, Xu W, Ai Q, et al. Sodium butyrate supplementation in high-soybean meal diets for turbot (Scophthalmus maximus L.): effects on inflammatory status, mucosal barriers and microbiota in the intestine. Fish & shellfish immunology. 2019;88:65-75. DOI: 10.1016/j.fsi.2019.02.064.]'},{id:"B103",body:'[Krome C, Schuele F, Jauncey K, Focken U. 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Effect of fermentation on the nutritive value of sesame seed meal in the diets for rohu, Labeo rohita (Hamilton), fingerlings. 1999. DOI: 10.1046/j.1365-2095.1999.00101.x.]'},{id:"B107",body:'[Lazzarotto V, Corraze G, Leprevost A, Quillet E, Dupont-Nivet M, Médale F. Three-year breeding cycle of rainbow trout (Oncorhynchus mykiss) fed a plant-based diet, totally free of marine resources: consequences for reproduction, fatty acid composition and progeny survival. PloS one. 2015;10(2):e0117609. DOI: 10.1371/journal.pone.0117609.]'},{id:"B108",body:'[Izquierdo M, Turkmen S, Montero D, Zamorano M, Afonso J, Karalazos V, et al. Nutritional programming through broodstock diets to improve utilization of very low fishmeal and fish oil diets in gilthead sea bream. Aquaculture. 2015;449:18-26. DOI: 10.1016/j.aquaculture.2015.03.032.]'},{id:"B109",body:'[Clarkson M, Migaud H, Metochis C, Vera LM, Leeming D, Tocher DR, et al. Early nutritional intervention can improve utilisation of vegetable-based diets in diploid and triploid Atlantic salmon (Salmo salar L.). British Journal of Nutrition. 2017;118(1):17-29. DOI: 10.1017/S0007114517001842.]'},{id:"B110",body:'[Overturf K, Barrows FT, Hardy RW. Effect and interaction of rainbow trout strain (Oncorhynchus mykiss) and diet type on growth and nutrient retention. Aquaculture Research. 2013;44(4):604-11. DOI: 10.1111/j.1365-2109.2011.03065.x.]'},{id:"B111",body:'[Abernathy J, Overturf K. Expression of antisense long noncoding RNAs as potential regulators in rainbow trout with different tolerance to plant-based diets. Animal biotechnology. 2019;30(1):87-94. DOI: 10.1080/10495398.2017.1401546.]'}],footnotes:[],contributors:[{corresp:null,contributorFullName:"Fateme Hekmatpour",address:null,affiliation:'- South Iran Aquaculture Research Centre, Iranian Fisheries Science Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Ahwaz, Iran
'},{corresp:"yes",contributorFullName:"Mansour Torfi Mozanzadeh",address:"m.torfi@areeo.ac.ir;, mansour.torfi@gmail.com",affiliation:'- South Iran Aquaculture Research Centre, Iranian Fisheries Science Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Ahwaz, Iran
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\n\nOut of all of the publishing options available to researchers, why choose to contribute your research to an IntechOpen Edited Volume? The reasons are simple. IntechOpen has worked exceptionally hard over the past years to fine tune the Open Access book publishing process and we continue to work hard to deliver the best for all of our contributors. The quality of published content is of utmost importance to us, followed closely by speed, and of course, availability and accessibility. To view current Open Access book projects that are Open for Submissions visit us here.
\n\nQUALITY CONTENT
\n\nOver the years we have learned what is important. What makes a difference to the researchers that work with us, what they value. Something that is very high not only on their lists, but our own, is the quality of the published content.
\n\nOur books contain scientific content written by two Nobel Prize winners, two Breakthrough Prize winners and 73 authors who are in the top 1% Most Cited.
\n\nWith regular submission for coverage in the single most important database, the Book Citation Index in the Web of Science™ Core Collection (BKCI), and no rejected submissions to date, over 43% of all Open Access books indexed in the BKCI are IntechOpen published books.
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\n\nThe need for up to date information available at the click of a mouse is one thing that sets IntechOpen apart. By developing our own technologies in order to streamline the publishing process, we are able to minimize the amount of time from initial submission of a manuscript to its final publication date, without compromising the rigor of the editorial and peer review process. This means that the research published stays relevant, and in this fast paced world, this is very important.
\n\nYOUR WORK, YOUR COPYRIGHT
\n\nThe utilization of CC licenses allow researchers to retain copyright to their work. Researchers are free to use, adapt and share all content they publish with us. You will never have to pay permission fees to reuse a part of an experiment that you worked so hard to complete and are free to build upon your own research and the research of others. The Edited Volume helps bring together research from all over the world and compiles that research into one book - accessible for all. The research presented in chapter one can inspire the author of chapter three to take his or her research to the next level. It is about sharing ideas, insights and knowledge.
\n\nCan collaboration be inspired by a publishing format? At IntechOpen, the answer is yes. The way the research is published, the way it is accessed, it’s all part of our mission to help academics make a greater impact by giving readers free access to all published work.
\n\nOur Open Access book collection includes:
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\n\nTo view current Open Access book projects that are Open for Submissions visit us here.
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Activation of cardiovascular CB1 receptors leads to depressed cardiac contractility and hypotension. Conversely, in most studies, the CB1 receptor antagonists are cardioprotective against ischemia–reperfusion injury, myocardial ischemia, heart failure, and cardiomyopathies. Evidence to date indicates that CB2 receptor activation is cardioprotective. CB2 receptor-mediated effects such as anti-inflammation and anti-fibrosis may be in part opposite to the actions of the CB1 receptor. The aim of this review is to up-date on recent experimental findings and controversies on the role of endocannabinoid system in the myocardial injury with emphasis on pathophysiological processes such as left ventricular remodeling, cardiac fibrosis, hypertrophy, and endothelial dysfunction. Recent experimental studies employing genetic deficiency of CB1 and CB2 receptors and endocannabinoid anandamide metabolizing enzymes are reviewed. Moreover, the protective mechanisms which are mediated by cannabinoid receptors during ischemic preconditioning as well as in the early and late phase after myocardial infarction are discussed in the context of possible therapeutic implications.",book:{id:"5222",slug:"cannabinoids-in-health-and-disease",title:"Cannabinoids in Health and Disease",fullTitle:"Cannabinoids in Health and Disease"},signatures:"Elena Kaschina",authors:[{id:"32266",title:"Dr.",name:"Elena",middleName:null,surname:"Kaschina",slug:"elena-kaschina",fullName:"Elena Kaschina"}]},{id:"50397",doi:"10.5772/62498",title:"Dietary Omega-6/Omega-3 and Endocannabinoids: Implications for Brain Health and Diseases",slug:"dietary-omega-6-omega-3-and-endocannabinoids-implications-for-brain-health-and-diseases",totalDownloads:2521,totalCrossrefCites:6,totalDimensionsCites:10,abstract:"Omega-3 (ω-3) and omega-6 (ω-6) are polyunsaturated fatty acids (PUFAs) that play critical role in human health and have to be provided by food. In the brain, PUFAs are also precursors of endocannabinoids. The aim of this chapter is to review the existing literature on how dietary PUFAs impact on the endocannabinoid system in the brain and what are the consequences for brain function and dysfunction. In this chapter, we will first describe how PUFAs enter the brain, what are their metabolism processes and roles in brain function. We will describe the pathways from PUFAs to endocannabinoid production. Then, we will review the literature on how dietary ω-6/ω-3 ratio impacts the endocannabinoid system, in terms of endocannabinoid levels, proteins and endocannabinoid-dependent synaptic plasticity. In the next part, we will describe what we know about the interactions between PUFAs and endocannabinoids in neurological and neuropsychiatric disorders. Finally, we will conclude on the possible implications of the interactions between dietary PUFAs and endocannabinoids in the normal and pathological brain. In particular, we will discuss how dietary PUFAs, as homeostatic regulators of endocannabinoids, can constitute interesting therapeutic strategies for the prevention and/or treatment of neurological disorders with endocannabinoids impairment.",book:{id:"5222",slug:"cannabinoids-in-health-and-disease",title:"Cannabinoids in Health and Disease",fullTitle:"Cannabinoids in Health and Disease"},signatures:"Clémentine Bosch-Bouju and Sophie Layé",authors:[{id:"178351",title:"Dr.",name:"Sophie",middleName:null,surname:"Layé",slug:"sophie-laye",fullName:"Sophie Layé"}]},{id:"50674",doi:"10.5772/63214",title:"Endocannabinoid Signaling in Neural Circuits of the Olfactory and Limbic System",slug:"endocannabinoid-signaling-in-neural-circuits-of-the-olfactory-and-limbic-system",totalDownloads:1600,totalCrossrefCites:1,totalDimensionsCites:8,abstract:"The endocannabinoid system with cannabinoid receptors, specifically cannabinoid receptor type 1 (CB1R), and their endogenous activators, the endocannabinoids, has emerged as an important neuromodulator system. Our understanding of the endocannabinoid system has significantly advanced in limbic system areas such as the hippocampus and the amygdala. However, the study of this signaling system in the olfactory pathway is still in its infancy. Here, we review the role of endocannabinoids as signaling molecules in activity-dependent regulation of dynamically changing neural networks in the limbic and olfactory system and the relevance of the endocannabinoid system for synaptic plasticity. We highlight the prospects for cannabinoid-based therapies in the treatment of various brain disorders and the role of endocannabinoids as neuroprotective agents. An increased understanding of cannabinoid signaling has the potential to pave the way for developing cannabis-related substances as medications.",book:{id:"5222",slug:"cannabinoids-in-health-and-disease",title:"Cannabinoids in Health and Disease",fullTitle:"Cannabinoids in Health and Disease"},signatures:"Thomas Heinbockel, Ze-Jun Wang, Edward A. Brown and Paul T.\nAustin",authors:[{id:"70569",title:"Dr.",name:"Thomas",middleName:null,surname:"Heinbockel",slug:"thomas-heinbockel",fullName:"Thomas Heinbockel"},{id:"185616",title:"Dr.",name:"Ze-Jun",middleName:null,surname:"Wang",slug:"ze-jun-wang",fullName:"Ze-Jun Wang"},{id:"185617",title:"Mr.",name:"Edward",middleName:null,surname:"Brown",slug:"edward-brown",fullName:"Edward Brown"},{id:"185618",title:"Mr.",name:"Paul",middleName:null,surname:"Austin",slug:"paul-austin",fullName:"Paul Austin"}]},{id:"50166",doi:"10.5772/62438",title:"Cannabinoids and Motor Control of the Basal Ganglia: Therapeutic Potential in Movement Disorders",slug:"cannabinoids-and-motor-control-of-the-basal-ganglia-therapeutic-potential-in-movement-disorders",totalDownloads:1585,totalCrossrefCites:1,totalDimensionsCites:4,abstract:"Cannabinoid receptors in the brain appear to be intimately involved in the motor control. Cannabinoid CB1 receptors are densely located in the basal ganglia (BG), a forebrain system that integrates cortical information to coordinate motor activity regulating signals. In fact, the administration of plant-derived, synthetic or endogenous cannabinoids produces several effects on motor function. These effects are paralleled to changes in the levels of different neurotransmitters in the BG, including GABA, dopamine and glutamate, all of which are important players in movement control.",book:{id:"5222",slug:"cannabinoids-in-health-and-disease",title:"Cannabinoids in Health and Disease",fullTitle:"Cannabinoids in Health and Disease"},signatures:"Teresa Morera-Herreras, Cristina Miguelez, Asier Aristieta, María Torrecilla, José Ángel Ruiz-Ortega and Luisa Ugedo",authors:[{id:"178735",title:"Dr.",name:"Teresa",middleName:null,surname:"Morera-Herreras",slug:"teresa-morera-herreras",fullName:"Teresa Morera-Herreras"},{id:"179364",title:"Dr.",name:"Maria",middleName:null,surname:"Torrecilla",slug:"maria-torrecilla",fullName:"Maria Torrecilla"},{id:"179365",title:"Dr.",name:"Cristina",middleName:null,surname:"Miguelez",slug:"cristina-miguelez",fullName:"Cristina Miguelez"},{id:"179366",title:"Dr.",name:"Asier",middleName:null,surname:"Aristieta",slug:"asier-aristieta",fullName:"Asier Aristieta"},{id:"179367",title:"Dr.",name:"Jose Angel",middleName:null,surname:"Ruiz-Ortega",slug:"jose-angel-ruiz-ortega",fullName:"Jose Angel Ruiz-Ortega"},{id:"179368",title:"Prof.",name:"Luisa",middleName:null,surname:"Ugedo",slug:"luisa-ugedo",fullName:"Luisa Ugedo"}]}],mostDownloadedChaptersLast30Days:[{id:"48746",title:"Anticancer Plants in Islamic Traditional Medicine",slug:"anticancer-plants-in-islamic-traditional-medicine",totalDownloads:2135,totalCrossrefCites:6,totalDimensionsCites:14,abstract:"Islamic Traditional Medicine (ITM) is a holistic and comprehensive medical school that has antecedents over 12 centuries ago.",book:{id:"4625",slug:"complementary-therapies-for-the-body-mind-and-soul",title:"Complementary Therapies for the Body, Mind and Soul",fullTitle:"Complementary Therapies for the Body, Mind and Soul"},signatures:"Behjat Javadi, Milad Iranshahy and Seyed Ahmad Emami",authors:[{id:"46265",title:"Dr.",name:"Seyed Ahmad",middleName:null,surname:"Emami",slug:"seyed-ahmad-emami",fullName:"Seyed Ahmad Emami"},{id:"177141",title:"Dr.",name:"Behjat",middleName:null,surname:"Javadi",slug:"behjat-javadi",fullName:"Behjat Javadi"},{id:"177142",title:"Dr.",name:"Milad",middleName:null,surname:"Iranshahy",slug:"milad-iranshahy",fullName:"Milad Iranshahy"}]},{id:"48731",title:"Animal Assisted Intervention for Rehabilitation Therapy and Psychotherapy",slug:"animal-assisted-intervention-for-rehabilitation-therapy-and-psychotherapy",totalDownloads:3239,totalCrossrefCites:1,totalDimensionsCites:3,abstract:"Animal-assisted Intervention (AAI) is a goal-oriented intervention that intentionally includes or incorporates animals in health, education, and human service for the purpose of therapeutic gains in humans. AAI incorporates human-animal teams in formal human service such as Animal-assisted Therapy (AAT) or Animal-assisted Education (AAE). Animal-assisted Activity (AAA) is the informal AAI often conducted on a volunteer basis by the human-animal team for motivational, educational, and recreational purposes. AAI could be used for rehabilitation therapy and psychotherapy for patients with various symptoms. AAI uses animals, mostly dogs, to aid in healing patients holistically. Dogs have an overwhelming gratitude and exuberance for life and this effect on people is astounding. Furthermore, AAI has been researched and its effectiveness on patients’ outcomes and healing is documented. With a soaring trend of the incorporation of complementary therapies into the mainstream of therapy and health care, animal-facilitated therapy has become a popular interest for the therapy team to integrate into a patient’s plan of therapy.",book:{id:"4625",slug:"complementary-therapies-for-the-body-mind-and-soul",title:"Complementary Therapies for the Body, Mind and Soul",fullTitle:"Complementary Therapies for the Body, Mind and Soul"},signatures:"Okjin Kim, Sunhwa Hong, Hyun-A Lee, Yung-Ho Chung and Si-Jong\nLee",authors:[{id:"174303",title:"Prof.",name:"Okjin",middleName:null,surname:"Kim",slug:"okjin-kim",fullName:"Okjin Kim"},{id:"174309",title:"Prof.",name:"Sunhwa",middleName:null,surname:"Hong",slug:"sunhwa-hong",fullName:"Sunhwa Hong"},{id:"174310",title:"Prof.",name:"Hyun-A",middleName:null,surname:"Lee",slug:"hyun-a-lee",fullName:"Hyun-A Lee"},{id:"175622",title:"Prof.",name:"Yung-Ho",middleName:null,surname:"Chung",slug:"yung-ho-chung",fullName:"Yung-Ho Chung"},{id:"175623",title:"Prof.",name:"Si-Jong",middleName:null,surname:"Lee",slug:"si-jong-lee",fullName:"Si-Jong Lee"}]},{id:"48527",title:"Role of Acupuncture in the Treatment of Drug Addiction",slug:"role-of-acupuncture-in-the-treatment-of-drug-addiction",totalDownloads:1692,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"This review systematically assessed the clinical evidence for and against acupuncture as a treatment for drug addiction. The existing scientific rationale and possible mechanisms for the effectiveness of acupuncture on drug addiction were also evaluated. We used computerized literature searches in English and Chinese and examined texts written before these computerized databases existed. We also used search terms of treatment and neurobiology for drug abuse and dependence. Acupuncture showed evidence for relevant neurobiological mechanisms in the treatment of drug addiction. Although positive findings regarding the use of acupuncture to treat drug dependence have been reported by many clinical studies, the data do not allow us to make conclusions that acupuncture was an effective treatment for drug addiction, given that many studies reviewed here were hampered by small numbers of patients, insufficient reporting of randomization and allocation concealment methods, and strength of the inference. 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A Bioenergetic Perspective",slug:"why-is-qi-invigorating-therapy-in-chinese-medicine-suitable-for-mitochondrial-diseases-a-bioenergeti",totalDownloads:2210,totalCrossrefCites:1,totalDimensionsCites:2,abstract:"The central player in bioenergetics is the mitochondrion. Bioenergetic dysfunction is emerging as a cornerstone for understanding the pathophysiology of mitochondrial diseases. Accompanying the depth of mitochondrial research and the rapid development of mitochondrial medicine, however, is rapid amplification of the number of mitochondrial diseases; mitochondrial dysfunction would undermine the function of cells, tissues, and organs, thereby causing cancer, diabetes, obesity, strokes, cardiovascular diseases, neurodegenerative diseases, and ageing, etc. Currently, there are no effective treatments; Western medicine is in crisis when it comes to mitochondrial diseases.",book:{id:"4625",slug:"complementary-therapies-for-the-body-mind-and-soul",title:"Complementary Therapies for the Body, Mind and Soul",fullTitle:"Complementary Therapies for the Body, Mind and Soul"},signatures:"Xing-Tai Li, Hai-Xue Kuang and Jia Zhao",authors:[{id:"44740",title:"Prof.",name:"Haixue",middleName:null,surname:"Kuang",slug:"haixue-kuang",fullName:"Haixue Kuang"},{id:"73821",title:"Dr.",name:"Xing-Tai",middleName:null,surname:"Li",slug:"xing-tai-li",fullName:"Xing-Tai Li"},{id:"122689",title:"Dr.",name:"Jia",middleName:null,surname:"Zhao",slug:"jia-zhao",fullName:"Jia Zhao"}]}],onlineFirstChaptersFilter:{topicId:"992",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:98,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:287,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:10,numberOfPublishedChapters:103,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:10,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"24",title:"Sustainable Development",doi:"10.5772/intechopen.100361",issn:null,scope:"\r\n\tTransforming our World: the 2030 Agenda for Sustainable Development endorsed by United Nations and 193 Member States, came into effect on Jan 1, 2016, to guide decision making and actions to the year 2030 and beyond. 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\r\n\r\n\t
\r\n\r\n\t2. Health and Wellbeing focusing on SDG 3 on Good Health and Wellbeing and SDG 6 on Clean Water and Sanitation
\r\n\r\n\t
\r\n\r\n\t3. Inclusivity and Social Equality involving SDG 4 on Quality Education, SDG 5 on Gender Equality, and SDG 16 on Peace, Justice and Strong Institutions
\r\n\r\n\t
\r\n\r\n\t4. Climate Change and Environmental Sustainability comprising SDG 13 on Climate Action, SDG 14 on Life Below Water, and SDG 15 on Life on Land
\r\n\r\n\t
\r\n\r\n\t5. Urban Planning and Environmental Management embracing SDG 7 on Affordable Clean Energy, SDG 9 on Industry, Innovation and Infrastructure, and SDG 11 on Sustainable Cities and Communities.
\r\n\r\n\t
\r\n\r\n\tThe series also seeks to support the use of cross cutting SDGs, as many of the goals listed above, targets and indicators are all interconnected to impact our lives and the decisions we make on a daily basis, making them impossible to tie to a single topic.
",coverUrl:"https://cdn.intechopen.com/series/covers/24.jpg",latestPublicationDate:"May 19th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:0,editor:{id:"262440",title:"Prof.",name:"Usha",middleName:null,surname:"Iyer-Raniga",slug:"usha-iyer-raniga",fullName:"Usha Iyer-Raniga",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRYSXQA4/Profile_Picture_2022-02-28T13:55:36.jpeg",biography:"Usha Iyer-Raniga is a professor in the School of Property and Construction Management at RMIT University. Usha co-leads the One Planet Network’s Sustainable Buildings and Construction Programme (SBC), a United Nations 10 Year Framework of Programmes on Sustainable Consumption and Production (UN 10FYP SCP) aligned with Sustainable Development Goal 12. The work also directly impacts SDG 11 on Sustainable Cities and Communities. She completed her undergraduate degree as an architect before obtaining her Masters degree from Canada and her Doctorate in Australia. Usha has been a keynote speaker as well as an invited speaker at national and international conferences, seminars and workshops. Her teaching experience includes teaching in Asian countries. She has advised Austrade, APEC, national, state and local governments. She serves as a reviewer and a member of the scientific committee for national and international refereed journals and refereed conferences. She is on the editorial board for refereed journals and has worked on Special Issues. Usha has served and continues to serve on the Boards of several not-for-profit organisations and she has also served as panel judge for a number of awards including the Premiers Sustainability Award in Victoria and the International Green Gown Awards. Usha has published over 100 publications, including research and consulting reports. Her publications cover a wide range of scientific and technical research publications that include edited books, book chapters, refereed journals, refereed conference papers and reports for local, state and federal government clients. She has also produced podcasts for various organisations and participated in media interviews. She has received state, national and international funding worth over USD $25 million. Usha has been awarded the Quarterly Franklin Membership by London Journals Press (UK). Her biography has been included in the Marquis Who's Who in the World® 2018, 2016 (33rd Edition), along with approximately 55,000 of the most accomplished men and women from around the world, including luminaries as U.N. Secretary-General Ban Ki-moon. In 2017, Usha was awarded the Marquis Who’s Who Lifetime Achiever Award.",institutionString:null,institution:{name:"RMIT University",institutionURL:null,country:{name:"Australia"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:9,paginationItems:[{id:"91",title:"Sustainable Economy and Fair Society",coverUrl:"https://cdn.intechopen.com/series_topics/covers/91.jpg",editor:{id:"181603",title:"Dr.",name:"Antonella",middleName:null,surname:"Petrillo",slug:"antonella-petrillo",fullName:"Antonella Petrillo",profilePictureURL:"https://mts.intechopen.com/storage/users/181603/images/system/181603.jpg",biography:"Antonella Petrillo is a Professor at the Department of Engineering of the University of Naples “Parthenope”, Italy. She received her Ph.D. in Mechanical Engineering from the University of Cassino. Her research interests include multi-criteria decision analysis, industrial plant, logistics, manufacturing and safety. She serves as an Associate Editor for the International Journal of the Analytic Hierarchy Process. She is a member of AHP Academy and a member of several editorial boards. She has over 160 Scientific Publications in International Journals and Conferences and she is the author of 5 books on Innovation and Decision Making in Industrial Applications and Engineering.",institutionString:null,institution:{name:"Parthenope University of Naples",institutionURL:null,country:{name:"Italy"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"179628",title:"Prof.",name:"Dima",middleName:null,surname:"Jamali",slug:"dima-jamali",fullName:"Dima Jamali",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSAIlQAO/Profile_Picture_2022-03-07T08:52:23.jpg",institutionString:null,institution:{name:"University of Sharjah",institutionURL:null,country:{name:"United Arab Emirates"}}},{id:"170206",title:"Prof.",name:"Dr. Orhan",middleName:null,surname:"Özçatalbaş",slug:"dr.-orhan-ozcatalbas",fullName:"Dr. Orhan Özçatalbaş",profilePictureURL:"https://mts.intechopen.com/storage/users/170206/images/system/170206.png",institutionString:null,institution:{name:"Akdeniz University",institutionURL:null,country:{name:"Turkey"}}},{id:"250347",title:"Associate Prof.",name:"Isaac",middleName:null,surname:"Oluwatayo",slug:"isaac-oluwatayo",fullName:"Isaac Oluwatayo",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRVIVQA4/Profile_Picture_2022-03-17T13:25:32.jpg",institutionString:null,institution:{name:"University of Venda",institutionURL:null,country:{name:"South Africa"}}},{id:"141386",title:"Prof.",name:"Jesús",middleName:null,surname:"López-Rodríguez",slug:"jesus-lopez-rodriguez",fullName:"Jesús López-Rodríguez",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRBNIQA4/Profile_Picture_2022-03-21T08:24:16.jpg",institutionString:null,institution:{name:"University of A Coruña",institutionURL:null,country:{name:"Spain"}}},{id:"208657",title:"Dr.",name:"Mara",middleName:null,surname:"Del Baldo",slug:"mara-del-baldo",fullName:"Mara Del Baldo",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRLMUQA4/Profile_Picture_2022-05-18T08:19:24.png",institutionString:"University of Urbino Carlo Bo",institution:null}]},{id:"92",title:"Health and Wellbeing",coverUrl:"https://cdn.intechopen.com/series_topics/covers/92.jpg",editor:{id:"348225",title:"Prof.",name:"Ann",middleName:null,surname:"Hemingway",slug:"ann-hemingway",fullName:"Ann Hemingway",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035LZFoQAO/Profile_Picture_2022-04-11T14:55:40.jpg",biography:"Professor Hemingway is a public health researcher, Bournemouth University, undertaking international and UK research focused on reducing inequalities in health outcomes for marginalised and excluded populations and more recently focused on equine assisted interventions.",institutionString:null,institution:{name:"Bournemouth University",institutionURL:null,country:{name:"United Kingdom"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"169536",title:"Dr.",name:"David",middleName:null,surname:"Claborn",slug:"david-claborn",fullName:"David Claborn",profilePictureURL:"https://mts.intechopen.com/storage/users/169536/images/system/169536.jpeg",institutionString:null,institution:{name:"Missouri State University",institutionURL:null,country:{name:"United States of America"}}},{id:"248594",title:"Ph.D.",name:"Jasneth",middleName:null,surname:"Mullings",slug:"jasneth-mullings",fullName:"Jasneth Mullings",profilePictureURL:"https://mts.intechopen.com/storage/users/248594/images/system/248594.jpeg",institutionString:"The University Of The West Indies - Mona Campus, Jamaica",institution:null},{id:"331299",title:"Prof.",name:"Pei-Shan",middleName:null,surname:"Liao",slug:"pei-shan-liao",fullName:"Pei-Shan Liao",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000032Fh2FQAS/Profile_Picture_2022-03-18T09:39:41.jpg",institutionString:"Research Center for Humanities and Social Sciences, Academia Sinica, Taiwan",institution:null}]},{id:"93",title:"Inclusivity and Social Equity",coverUrl:"https://cdn.intechopen.com/series_topics/covers/93.jpg",editor:{id:"210060",title:"Prof. Dr.",name:"Ebba",middleName:null,surname:"Ossiannilsson",slug:"ebba-ossiannilsson",fullName:"Ebba Ossiannilsson",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6LkBQAU/Profile_Picture_2022-02-28T13:31:48.png",biography:'Professor Dr. Ebba Ossiannilsson is an independent researcher, expert, consultant, quality auditor and influencer in the fields of open, flexible online and distance learning (OFDL) and the "new normal". Her focus is on quality, innovation, leadership, and personalised learning. She works primarily at the strategic and policy levels, both nationally and internationally, and with key international organisations. She is committed to promoting and improving OFDL in the context of SDG4 and the future of education. Ossiannilsson has more than 20 years of experience in her current field, but more than 40 years in the education sector. She works as a reviewer and expert for the European Commission and collaborates with the Joint Research Centre for Quality in Open Education. Ossiannilsson also collaborates with ITCILO and ICoBC (International Council on Badges and Credentials). She is a member of the ICDE Board of Directors and has previously served on the boards of EDEN and EUCEN. Ossiannilsson is a quality expert and reviewer for ICDE, EDEN and the EADTU. She chairs the ICDE OER Advocacy Committee and is a member of the ICDE Quality Network. She is regularly invited as a keynote speaker at conferences. She is a guest editor for several special issues and a member of the editorial board of several scientific journals. She has published more than 200 articles and is currently working on book projects in the field of OFDL. Ossiannilsson is a visiting professor at several international universities and was recently appointed Professor and Research Fellow at Victoria University of Wellington, NZ. Ossiannilsson has been awarded the following fellowships: EDEN Fellows, EDEN Council of Fellows, and Open Education Europe. She is a ICDE OER Ambassador, Open Education Europe Ambassador, GIZ Ambassador for Quality in Digital Learning, and part of the Globe-Community of Digital Learning and Champion of SPARC Europe. On a national level, she is a quality developer at the Swedish Institute for Standards (SIS) and for ISO. She is a member of the Digital Skills and Jobs Coalition Sweden and Vice President of the Swedish Association for Distance Education. She is currently working on a government initiative on quality in distance education at the National Council for Higher Education. She holds a Ph.D. from the University of Oulu, Finland.',institutionString:"Swedish Association for Distance Education, Sweden",institution:null},editorTwo:null,editorThree:null,editorialBoard:[{id:"320585",title:"Ph.D.",name:"Deborah",middleName:null,surname:"Young",slug:"deborah-young",fullName:"Deborah Young",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00002vZLcTQAW/Profile_Picture_2022-05-10T08:30:47.jpg",institutionString:"Empowering Communities Globally",institution:null},{id:"348038",title:"Associate Prof.",name:"Feyza",middleName:null,surname:"Bhatti",slug:"feyza-bhatti",fullName:"Feyza Bhatti",profilePictureURL:"https://mts.intechopen.com/storage/users/348038/images/system/348038.jpg",institutionString:"Girne American University",institution:{name:"Girne American University",institutionURL:null,country:{name:"Cyprus"}}},{id:"128665",title:"Prof.",name:"Man-Chung",middleName:null,surname:"Chiu",slug:"man-chung-chiu",fullName:"Man-Chung Chiu",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bR9OrQAK/Profile_Picture_2022-03-09T08:36:59.JPG",institutionString:null,institution:{name:"Beijing Normal University",institutionURL:null,country:{name:"China"}}}]},{id:"95",title:"Urban Planning and Environmental Management",coverUrl:"https://cdn.intechopen.com/series_topics/covers/95.jpg",editor:{id:"181079",title:"Dr.",name:"Christoph",middleName:null,surname:"Lüthi",slug:"christoph-luthi",fullName:"Christoph Lüthi",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRHSqQAO/Profile_Picture_2022-04-12T15:51:33.png",biography:"Dr. Christoph Lüthi is an urban infrastructure planner with over 25 years of experience in planning and design of urban infrastructure in middle and low-income countries. 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He is also a faculty member in the Molecular Oncology Program. He obtained his MSc and Ph.D. at Oregon State University and Texas Tech University, respectively. He pursued his postdoctoral studies at Rutgers University Medical School and the National Institutes of Health (NIH/NIDDK), USA. His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRNVJQA4/Profile_Picture_2022-03-07T13:23:04.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals. His research interests include biochemistry, oxidative stress, reactive species, antioxidants, lipid peroxidation, inflammation, reproductive hormones, phenolic compounds, female infertility.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Associate Prof.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. He has many years of experience in research and teaching in various fields of medicine, is an author/co-author of more than 200 scientific publications, 13 patents, 15 medical books/chapters, including Chapter in Book «Metabolomics», IntechOpen, 2020 «Metabolomic Discovery of Microbiota Dysfunction as the Cause of Pathology».",institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"199461",title:"Prof.",name:"Natalia V.",middleName:null,surname:"Beloborodova",slug:"natalia-v.-beloborodova",fullName:"Natalia V. Beloborodova",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/199461/images/system/199461.jpg",biography:'Natalia Vladimirovna Beloborodova was educated at the Pirogov Russian National Research Medical University, with a degree in pediatrics in 1980, a Ph.D. in 1987, and a specialization in Clinical Microbiology from First Moscow State Medical University in 2004. She has been a Professor since 1996. Currently, she is the Head of the Laboratory of Metabolism, a division of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russian Federation. N.V. Beloborodova has many years of clinical experience in the field of intensive care and surgery. She studies infectious complications and sepsis. She initiated a series of interdisciplinary clinical and experimental studies based on the concept of integrating human metabolism and its microbiota. Her scientific achievements are widely known: she is the recipient of the Marie E. Coates Award \\"Best lecturer-scientist\\" Gustafsson Fund, Karolinska Institutes, Stockholm, Sweden, and the International Sepsis Forum Award, Pasteur Institute, Paris, France (2014), etc. Professor N.V. Beloborodova wrote 210 papers, five books, 10 chapters and has edited four books.',institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"354260",title:"Ph.D.",name:"Tércio Elyan",middleName:"Azevedo",surname:"Azevedo Martins",slug:"tercio-elyan-azevedo-martins",fullName:"Tércio Elyan Azevedo Martins",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/354260/images/16241_n.jpg",biography:"Graduated in Pharmacy from the Federal University of Ceará with the modality in Industrial Pharmacy, Specialist in Production and Control of Medicines from the University of São Paulo (USP), Master in Pharmaceuticals and Medicines from the University of São Paulo (USP) and Doctor of Science in the program of Pharmaceuticals and Medicines by the University of São Paulo. Professor at Universidade Paulista (UNIP) in the areas of chemistry, cosmetology and trichology. Assistant Coordinator of the Higher Course in Aesthetic and Cosmetic Technology at Universidade Paulista Campus Chácara Santo Antônio. Experience in the Pharmacy area, with emphasis on Pharmacotechnics, Pharmaceutical Technology, Research and Development of Cosmetics, acting mainly on topics such as cosmetology, antioxidant activity, aesthetics, photoprotection, cyclodextrin and thermal analysis.",institutionString:null,institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"334285",title:"Ph.D. Student",name:"Sameer",middleName:"Kumar",surname:"Jagirdar",slug:"sameer-jagirdar",fullName:"Sameer Jagirdar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334285/images/14691_n.jpg",biography:"I\\'m a graduate student at the center for biosystems science and engineering at the Indian Institute of Science, Bangalore, India. I am interested in studying host-pathogen interactions at the biomaterial interface.",institutionString:null,institution:{name:"Indian Institute of Science Bangalore",country:{name:"India"}}},{id:"329795",title:"Dr.",name:"Mohd Aftab",middleName:"Aftab",surname:"Siddiqui",slug:"mohd-aftab-siddiqui",fullName:"Mohd Aftab Siddiqui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329795/images/15648_n.jpg",biography:"Dr. Mohd Aftab Siddiqui is currently working as Assistant Professor in the Faculty of Pharmacy, Integral University, Lucknow for the last 6 years. He has completed his Doctor in Philosophy (Pharmacology) in 2020 from Integral University, Lucknow. He completed his Bachelor in Pharmacy in 2013 and Master in Pharmacy (Pharmacology) in 2015 from Integral University, Lucknow. He is the gold medalist in Bachelor and Master degree. He qualified GPAT -2013, GPAT -2014, and GPAT 2015. His area of research is Pharmacological screening of herbal drugs/ natural products in liver and cardiac diseases. He has guided many M. Pharm. research projects. He has many national and international publications.",institutionString:"Integral University",institution:null},{id:"255360",title:"Dr.",name:"Usama",middleName:null,surname:"Ahmad",slug:"usama-ahmad",fullName:"Usama Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255360/images/system/255360.png",biography:"Dr. Usama Ahmad holds a specialization in Pharmaceutics from Amity University, Lucknow, India. He received his Ph.D. degree from Integral University. Currently, he’s working as an Assistant Professor of Pharmaceutics in the Faculty of Pharmacy, Integral University. From 2013 to 2014 he worked on a research project funded by SERB-DST, Government of India. He has a rich publication record with more than 32 original articles published in reputed journals, 3 edited books, 5 book chapters, and a number of scientific articles published in ‘Ingredients South Asia Magazine’ and ‘QualPharma Magazine’. He is a member of the American Association for Cancer Research, International Association for the Study of Lung Cancer, and the British Society for Nanomedicine. Dr. Ahmad’s research focus is on the development of nanoformulations to facilitate the delivery of drugs that aim to provide practical solutions to current healthcare problems.",institutionString:"Integral University",institution:{name:"Integral University",country:{name:"India"}}},{id:"30568",title:"Prof.",name:"Madhu",middleName:null,surname:"Khullar",slug:"madhu-khullar",fullName:"Madhu Khullar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/30568/images/system/30568.jpg",biography:"Dr. Madhu Khullar is a Professor of Experimental Medicine and Biotechnology at the Post Graduate Institute of Medical Education and Research, Chandigarh, India. She completed her Post Doctorate in hypertension research at the Henry Ford Hospital, Detroit, USA in 1985. She is an editor and reviewer of several international journals, and a fellow and member of several cardiovascular research societies. Dr. Khullar has a keen research interest in genetics of hypertension, and is currently studying pharmacogenetics of hypertension.",institutionString:"Post Graduate Institute of Medical Education and Research",institution:{name:"Post Graduate Institute of Medical Education and Research",country:{name:"India"}}},{id:"223233",title:"Prof.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/223233/images/system/223233.png",biography:"Xianquan Zhan received his MD and Ph.D. in Preventive Medicine at West China University of Medical Sciences. He received his post-doctoral training in oncology and cancer proteomics at the Central South University, China, and the University of Tennessee Health Science Center (UTHSC), USA. He worked at UTHSC and the Cleveland Clinic in 2001–2012 and achieved the rank of associate professor at UTHSC. Currently, he is a full professor at Central South University and Shandong First Medical University, and an advisor to MS/PhD students and postdoctoral fellows. He is also a fellow of the Royal Society of Medicine and European Association for Predictive Preventive Personalized Medicine (EPMA), a national representative of EPMA, and a member of the American Society of Clinical Oncology (ASCO) and the American Association for the Advancement of Sciences (AAAS). He is also the editor in chief of International Journal of Chronic Diseases & Therapy, an associate editor of EPMA Journal, Frontiers in Endocrinology, and BMC Medical Genomics, and a guest editor of Mass Spectrometry Reviews, Frontiers in Endocrinology, EPMA Journal, and Oxidative Medicine and Cellular Longevity. He has published more than 148 articles, 28 book chapters, 6 books, and 2 US patents in the field of clinical proteomics and biomarkers.",institutionString:"Shandong First Medical University",institution:{name:"Affiliated Hospital of Shandong Academy of Medical Sciences",country:{name:"China"}}},{id:"297507",title:"Dr.",name:"Charles",middleName:"Elias",surname:"Assmann",slug:"charles-assmann",fullName:"Charles Assmann",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/297507/images/system/297507.jpg",biography:"Charles Elias Assmann is a biologist from Federal University of Santa Maria (UFSM, Brazil), who spent some time abroad at the Ludwig-Maximilians-Universität München (LMU, Germany). He has Masters Degree in Biochemistry (UFSM), and is currently a PhD student at Biochemistry at the Department of Biochemistry and Molecular Biology of the UFSM. His areas of expertise include: Biochemistry, Molecular Biology, Enzymology, Genetics and Toxicology. He is currently working on the following subjects: Aluminium toxicity, Neuroinflammation, Oxidative stress and Purinergic system. Since 2011 he has presented more than 80 abstracts in scientific proceedings of national and international meetings. Since 2014, he has published more than 20 peer reviewed papers (including 4 reviews, 3 in Portuguese) and 2 book chapters. He has also been a reviewer of international journals and ad hoc reviewer of scientific committees from Brazilian Universities.",institutionString:"Universidade Federal de Santa Maria",institution:{name:"Universidade Federal de Santa Maria",country:{name:"Brazil"}}},{id:"217850",title:"Dr.",name:"Margarete Dulce",middleName:null,surname:"Bagatini",slug:"margarete-dulce-bagatini",fullName:"Margarete Dulce Bagatini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217850/images/system/217850.jpeg",biography:"Dr. Margarete Dulce Bagatini is an associate professor at the Federal University of Fronteira Sul/Brazil. She has a degree in Pharmacy and a PhD in Biological Sciences: Toxicological Biochemistry. She is a member of the UFFS Research Advisory Committee\nand a member of the Biovitta Research Institute. She is currently:\nthe leader of the research group: Biological and Clinical Studies\nin Human Pathologies, professor of postgraduate program in\nBiochemistry at UFSC and postgraduate program in Science and Food Technology at\nUFFS. She has experience in the area of pharmacy and clinical analysis, acting mainly\non the following topics: oxidative stress, the purinergic system and human pathologies, being a reviewer of several international journals and books.",institutionString:"Universidade Federal da Fronteira Sul",institution:{name:"Universidade Federal da Fronteira Sul",country:{name:"Brazil"}}},{id:"226275",title:"Ph.D.",name:"Metin",middleName:null,surname:"Budak",slug:"metin-budak",fullName:"Metin Budak",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/226275/images/system/226275.jfif",biography:"Metin Budak, MSc, PhD is an Assistant Professor at Trakya University, Faculty of Medicine. He has been Head of the Molecular Research Lab at Prof. Mirko Tos Ear and Hearing Research Center since 2018. His specializations are biophysics, epigenetics, genetics, and methylation mechanisms. He has published around 25 peer-reviewed papers, 2 book chapters, and 28 abstracts. He is a member of the Clinical Research Ethics Committee and Quantification and Consideration Committee of Medicine Faculty. His research area is the role of methylation during gene transcription, chromatin packages DNA within the cell and DNA repair, replication, recombination, and gene transcription. His research focuses on how the cell overcomes chromatin structure and methylation to allow access to the underlying DNA and enable normal cellular function.",institutionString:"Trakya University",institution:{name:"Trakya University",country:{name:"Turkey"}}},{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",slug:"anca-pantea-stoian",fullName:"Anca Pantea Stoian",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",biography:"Anca Pantea Stoian is a specialist in diabetes, nutrition, and metabolic diseases as well as health food hygiene. She also has competency in general ultrasonography.\n\nShe is an associate professor in the Diabetes, Nutrition and Metabolic Diseases Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. She has been chief of the Hygiene Department, Faculty of Dentistry, at the same university since 2019. Her interests include micro and macrovascular complications in diabetes and new therapies. Her research activities focus on nutritional intervention in chronic pathology, as well as cardio-renal-metabolic risk assessment, and diabetes in cancer. She is currently engaged in developing new therapies and technological tools for screening, prevention, and patient education in diabetes. \n\nShe is a member of the European Association for the Study of Diabetes, Cardiometabolic Academy, CEDA, Romanian Society of Diabetes, Nutrition and Metabolic Diseases, Romanian Diabetes Federation, and Association for Renal Metabolic and Nutrition studies. She has authored or co-authored 160 papers in national and international peer-reviewed journals.",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",country:{name:"Romania"}}},{id:"279792",title:"Dr.",name:"João",middleName:null,surname:"Cotas",slug:"joao-cotas",fullName:"João Cotas",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279792/images/system/279792.jpg",biography:"Graduate and master in Biology from the University of Coimbra.\n\nI am a research fellow at the Macroalgae Laboratory Unit, in the MARE-UC – Marine and Environmental Sciences Centre of the University of Coimbra. My principal function is the collection, extraction and purification of macroalgae compounds, chemical and bioactive characterization of the compounds and algae extracts and development of new methodologies in marine biotechnology area. \nI am associated in two projects: one consists on discovery of natural compounds for oncobiology. The other project is the about the natural compounds/products for agricultural area.\n\nPublications:\nCotas, J.; Figueirinha, A.; Pereira, L.; Batista, T. 2018. An analysis of the effects of salinity on Fucus ceranoides (Ochrophyta, Phaeophyceae), in the Mondego River (Portugal). Journal of Oceanology and Limnology. in press. DOI: 10.1007/s00343-019-8111-3",institutionString:"Faculty of Sciences and Technology of University of Coimbra",institution:null},{id:"279788",title:"Dr.",name:"Leonel",middleName:null,surname:"Pereira",slug:"leonel-pereira",fullName:"Leonel Pereira",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279788/images/system/279788.jpg",biography:"Leonel Pereira has an undergraduate degree in Biology, a Ph.D. in Biology (specialty in Cell Biology), and a Habilitation degree in Biosciences (specialization in Biotechnology) from the Faculty of Science and Technology, University of Coimbra, Portugal, where he is currently a professor. In addition to teaching at this university, he is an integrated researcher at the Marine and Environmental Sciences Center (MARE), Portugal. His interests include marine biodiversity (algae), marine biotechnology (algae bioactive compounds), and marine ecology (environmental assessment). Since 2008, he has been the author and editor of the electronic publication MACOI – Portuguese Seaweeds Website (www.seaweeds.uc.pt). He is also a member of the editorial boards of several scientific journals. Dr. Pereira has edited or authored more than 20 books, 100 journal articles, and 45 book chapters. He has given more than 100 lectures and oral communications at various national and international scientific events. He is the coordinator of several national and international research projects. In 1998, he received the Francisco de Holanda Award (Honorable Mention) and, more recently, the Mar Rei D. Carlos award (18th edition). He is also a winner of the 2016 CHOICE Award for an outstanding academic title for his book Edible Seaweeds of the World. In 2020, Dr. Pereira received an Honorable Mention for the Impact of International Publications from the Web of Science",institutionString:"University of Coimbra",institution:{name:"University of Coimbra",country:{name:"Portugal"}}},{id:"61946",title:"Dr.",name:"Carol",middleName:null,surname:"Bernstein",slug:"carol-bernstein",fullName:"Carol Bernstein",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/61946/images/system/61946.jpg",biography:"Carol Bernstein received her PhD in Genetics from the University of California (Davis). She was a faculty member at the University of Arizona College of Medicine for 43 years, retiring in 2011. Her research interests focus on DNA damage and its underlying role in sex, aging and in the early steps of initiation and progression to cancer. In her research, she had used organisms including bacteriophage T4, Neurospora crassa, Schizosaccharomyces pombe and mice, as well as human cells and tissues. She authored or co-authored more than 140 scientific publications, including articles in major peer reviewed journals, book chapters, invited reviews and one book.",institutionString:"University of Arizona",institution:{name:"University of Arizona",country:{name:"United States of America"}}},{id:"182258",title:"Dr.",name:"Ademar",middleName:"Pereira",surname:"Serra",slug:"ademar-serra",fullName:"Ademar Serra",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/182258/images/system/182258.jpeg",biography:"Dr. Serra studied Agronomy on Universidade Federal de Mato Grosso do Sul (UFMS) (2005). He received master degree in Agronomy, Crop Science (Soil fertility and plant nutrition) (2007) by Universidade Federal da Grande Dourados (UFGD), and PhD in agronomy (Soil fertility and plant nutrition) (2011) from Universidade Federal da Grande Dourados / Escola Superior de Agricultura Luiz de Queiroz (UFGD/ESALQ-USP). Dr. Serra is currently working at Brazilian Agricultural Research Corporation (EMBRAPA). His research focus is on mineral nutrition of plants, crop science and soil science. Dr. Serra\\'s current projects are soil organic matter, soil phosphorus fractions, compositional nutrient diagnosis (CND) and isometric log ratio (ilr) transformation in compositional data analysis.",institutionString:"Brazilian Agricultural Research Corporation",institution:{name:"Brazilian Agricultural Research Corporation",country:{name:"Brazil"}}}]}},subseries:{item:{id:"14",type:"subseries",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). 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Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,series:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983"},editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",slug:"ana-isabel-flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",slug:"christian-palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",slug:"francisco-javier-martin-romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},onlineFirstChapters:{paginationCount:17,paginationItems:[{id:"81647",title:"Diabetes and Epigenetics",doi:"10.5772/intechopen.104653",signatures:"Rasha A. 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