Comparison of Means and Standard Deviations of Age and Treatment Period in the Root Resorption and Control Groups
\r\n\t(i) Quantum dots of very high-quality optical applications, Quantum dot light-emitting diodes (QD-LED) and ‘QD-White LED’, Quantum dot photodetectors (QDPs), Quantum dot solar cells (Photovoltaics).
\r\n\r\n\t(ii) Quantum Computing (quantum bits or ‘qubits’), (vii) The Future of Quantum Dots (broad range of real-time applications, magnetic quantum dots & graphene quantum dots), Superconducting Loop, Quantum Entanglement, Quantum Fingerprints.
\r\n\r\n\t(iii) Biomedical and Environmental Applications (to study intracellular processes, tumor targeting, in vivo observation of cell trafficking, diagnostics and cellular imaging at high resolutions), Bioconjugation, Cell Imaging, Photoelectrochemical Immunosensor, Membranes and Bacterial Cells, Resonance Energy-Transfer Processes, Evaluation of Drinking Water Quality, Water and Wastewater Treatment, Pollutant Control.
",isbn:"978-1-80356-594-1",printIsbn:"978-1-80356-593-4",pdfIsbn:"978-1-80356-595-8",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,hash:"0dd5611c62c91569bd2819e68852002a",bookSignature:"Prof. Jagannathan Thirumalai",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11756.jpg",keywords:"LED, Organic LEDs, Dyes & Pigments, Solar Cells, Laser Photonics, Electronic Switching Devices, Qubits, Josephson Junction, Bioconjugation, Cell Imaging, Photoelectrochemical Immunosensor, Membranes, and Bacterial Cells",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 16th 2022",dateEndSecondStepPublish:"May 27th 2022",dateEndThirdStepPublish:"July 26th 2022",dateEndFourthStepPublish:"October 14th 2022",dateEndFifthStepPublish:"December 13th 2022",remainingDaysToSecondStep:"10 days",secondStepPassed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"Dr. J. Thirumalai received his Ph.D. from Alagappa University, Karaikudi, He was also awarded the Post-doctoral Fellowship from Pohang University of Science and Technology (POSTECH), the Republic of Korea. His research interests focus on luminescence, self-assembled nanomaterials, and thin-film optoelectronic devices. He has published more than 60 SCOPUS/ISI indexed papers and 11 book chapters, edited 4 books, and member of several national and international societies like RSC, OSA, etc. His h-index is 19.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"99242",title:"Prof.",name:"Jagannathan",middleName:null,surname:"Thirumalai",slug:"jagannathan-thirumalai",fullName:"Jagannathan Thirumalai",profilePictureURL:"https://mts.intechopen.com/storage/users/99242/images/system/99242.png",biography:"Dr. J. Thirumalai received his Ph.D. from Alagappa University, Karaikudi in 2010. He was also awarded the Post-doctoral Fellowship from Pohang University of Science and Technology (POSTECH), Republic of Korea, in 2013. He worked as Assistant Professor of Physics, B.S. Abdur Rahman University, Chennai, India (2011 to 2016). Currently, he is working as Senior Assistant Professor of Physics, Srinivasa Ramanujan Centre, SASTRA Deemed University, Kumbakonam (T.N.), India. His research interests focus on luminescence, self-assembled nanomaterials, and thin film opto-electronic devices. He has published more than 60 SCOPUS/ISI indexed papers and 11 book chapters, edited 4 books and member in several national and international societies like RSC, OSA, etc. Currently, he served as a principal investigator for a funded project towards the application of luminescence based thin film opto-electronic devices, funded by the Science and Engineering Research Board (SERB), India. As an expert in opto-electronics and nanotechnology area, he has been invited as external and internal examiners to MSc and PhD theses, invited to give talk in some forum, review papers for international and national journals.",institutionString:"SASTRA University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"10",totalChapterViews:"0",totalEditedBooks:"6",institution:null}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"17",title:"Nanotechnology and Nanomaterials",slug:"nanotechnology-and-nanomaterials"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"347258",firstName:"Marica",lastName:"Novakovic",middleName:null,title:"Ms.",imageUrl:"//cdnintech.com/web/frontend/www/assets/author.svg",email:"marica@intechopen.com",biography:null}},relatedBooks:[{type:"book",id:"5348",title:"Luminescence",subtitle:"An 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However, an undesirable and unexpected result of orthodontic treatment is the orthodontically induced tooth resorption (Fig. 1). The development of excessive root resorption during orthodontic treatment is considered an adverse side effect of the orthodontic tooth movement. Many factors have been investigated to explain the orthodontically induced root resorption observed among orthodontic patients. However, there are still controversies over this issue.
An undesirable and unexpected result of orthodontic treatment (A) Before treatment, (B) After treatment.
Davidovitch et al. [1] hypothesized that individuals who have a history of immune diseases including allergy, asthma, and systemic diseases may be at a high level of risk for developing excessive root resorption during the course of orthodontic treatment. To confirm the association between allergy and the orthodontically induced root resorption, we designed the epidemiological clinical study. Moreover, to extrapolate the direct effect of allergy on the orthodontically induced root resorption, which is difficult to prove in the clinical study design, we conducted experimental animal study using rats.
In this chapter, we refer at the beginning to the current knowledge of root resorption and then focus on the risk factors inherent in individuals to the external root resorption (ERR), especially allergic diseases as a possible risk factor of ERR.
The presence of root resorption is common after orthodontic treatment [2]. Prevalence of root resorption following orthodontic treatment has been reported from 0% to 100% [3-5]. Thus, the prevalence varies widely due to differing methods of reporting root resorption, differing radiographic technique and interpretation, and individual susceptibility [2]. Close radiographic examination revealed that some loss of root length occurred in nearly every orthodontic patient. Moderate to severe apical root resorption (>2 mm, <1/3 of the root length) has been found in 10-17% of orthodontically treated patients [6,7] and excessive root resorption (>1/3 of the root length) in 1-5% [8,9]. Phillips [10] stated apical root resorption exceeding one-fourth of the original root length was shown in 1.5 % of maxillary central incisors and in 2.2 % of lateral incisors.
Regardless of patient-related or treatment-related factors, the maxillary incisors likely indicated more root resorption than the other teeth, followed by the mandibular incisors and first molars [11]. However, every tooth is capable of root resorption during orthodontic treatment.
Many studies have shown a distinct positive correlation between the applied orthodontic force and the amount of root resorption in both animal studies and clinical studies [12-16]. Owman-Mall et al. [17,18] reported that there were large individual variations in the amount of root resorption even when the same degree of orthodontic force was applied. They speculated that root resorption would not be very force-sensitive.
Several investigations revealed that the duration of force was one of the risk factors of orthodontically induced root resorption [19,20]. Some claimed that the duration of force application was an even more critical factor than the degree of orthodontic force [21,22]. Interaction between magnitude and duration of force would play an important role for orthodontic treatment-related risk factors. Large individual variations still existed even in well-controlled research design [17,18].
Besides orthodontic treatment-related factors, patient-related risk factors have also been discussed previously. This includes systemic diseases [23], nutrition [24,25], age [26], trauma [10,27], previous root resorption [28,29], gender [20,28], nail biting habits [30], root morphology [31], endodontically treated teeth [32], gingivitis [9], allergy [33-35], and medications [36]. Al-Qawasmi et al. [37] identified an interleukin (IL)-1β polymorphism in orthodontically treated individuals as having a role in the genetic influence on external apical root resorption.
Teeth are relocated under the dynamic balance of bone metabolism. The role of bone cells, including osteocytes, osteoblasts, and osteoclasts, are to resorb alveolar bone in sites of mechanically compressed tissues, thus enabling dental roots in these locations to move in the direction of the applied orthodontic force [38]. In these sites of tissue compression, one finds infiltrations of inflammatory cells followed by multinucleated osteocalsts and odontoclasts engaged in resorbing the dental roots [39,40]. Osteoclasts and their mononucleated progenitors are derived from the monocyte/macrophage lineage [41]. Osteoclasts and odontoclasts have a similar histochemical appearance; we defined here the multinucleated cells faced to tooth root as odontoclasts.
Patients with asthma and rhinitis share common physiology including heightened bronchial hyperresponsiveness and heightened reactivity to a variety of stimuli. Immunopathology of allergic rhinitis is also similar with a predominance of T-helper type 2 inflammation and tissue eosinophilia [42]. Allergy and asthma trigger the various associated biological, cellular, and molecular events with inflammation, such as increased vascular permeability, vasodilatation, cellular migration, increased mucus secretion, bronchoconstriction, structural changes of airway architecture, decline in pulmonary functions, release of intracellular mediators, increased formation of reactive oxygen species, cartilage degradation, and loss of function [43].
During orthodontic tooth movement, inflammation appears to be the main mechanism whereby immune cells and blood plasma reach the periodontal ligament of mechanically loaded teeth [44]. Apparently, strained sensory nerve endings in close proximity to periodontal ligament small blood vessels and capillaries release signal molecules such as substance P, vasoactive intestinal peptide, and calcitonin gene-related peptide [45]. Substance P stimulates endothelial cells to adhere to circulating leukocytes and promote their migration to the extravascular space, where they secrete a variety of cytokines, such as interleukins and tumor necrosis factors (TNFs).
TNF-α and IL-1β play an important role in bone pathophysiology [46,47] and have been suggested to be involved in orthodontic tooth movement [48]. These inflammatory cytokines induce local expression of receptor activator of nuclear factor-κB ligand (RANKL), which is critical for the terminal differentiation of osteoclast precursor cells [49].
Furthermore, leukotrienes, which are metabolites of arachidonic acid, are potent lipid mediators that play an important role in a variety of allergic and inflammatory reactions and comprise several products of the 5-lipoxygenase (5-LOX) pathway [50–52]. Leukotrienes are produced by activated leukocytes in inflammatory diseases such as bronchial asthma and rheumatoid arthritis [53,54]. LTB4 is known to play an important role in the allergic reactions induced by ovalbumin (OVA) in rodents [55] and has been suggested to modulate bone metabolism by increasing osteoclastic bone resorption [56].
In light of these facts, we hypothesize that patients with allergic diseases such as food allergy, allergic asthma, allergic rhinitis, and allergic conjunctivitis are susceptible to orthodontic root resorption. We show the scheme to explain the hypothesis of orthodontically induced root resorption due to the risk factor of allergy (Fig. 2).
Scheme of possible explanation of the orthodontically induced root resorption due to the risk factor of allergy.
At first, we report the possible association between excessive root resorption during the orthodontic tooth movement and allergy using clinical study design. Next, we extrapolate the effect of allergy on orthodontically induced root resorption using animal models.
The records of 3500 patients were obtained from the Section of Orthodontics, Kyushu University Hospital, Fukuoka, Japan. All these patients have completed their course of orthodontic treatment. In this sample, 100 individuals were found to have developed excessive resorption of dental roots. In the root resorption group, 29 subjects were males and 71 were females (Table 1). A control group was selected from the remaining patients of this sample who did not display any significant radiographic evidence for dental root resorption in the light of root morphology at the end of their orthodontic treatment. Each individual in the control group was pair-matched with one in the root resorption group based on age, gender, treatment period, and the type of malocclusion (Table 1).
\n\t\t\t | \n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t
Root resorption group | \n\t\t\t\n\t\t\t | \n\t\t\t | \n\t\t |
Male | \n\t\t\t29 | \n\t\t\t18.9 ± 5.4 | \n\t\t\t2.9 ± 0.8 | \n\t\t
Female | \n\t\t\t71 | \n\t\t\t17.3 ± 6.5 | \n\t\t\t3.1 ± 1.1 | \n\t\t
Total | \n\t\t\t100 | \n\t\t\t17.8 ± 6.2 | \n\t\t\t3.1 ± 1.1 | \n\t\t
Control group | \n\t\t\t\n\t\t\t | \n\t\t\t | \n\t\t |
Male | \n\t\t\t29 | \n\t\t\t17.9 ± 6.1 | \n\t\t\t2.8 ± 0.8 | \n\t\t
Female | \n\t\t\t71 | \n\t\t\t18.2 ± 4.8 | \n\t\t\t2.9 ± 0.8 | \n\t\t
Total | \n\t\t\t100 | \n\t\t\t18.1 ± 5.2 | \n\t\t\t2.9 ± 0.8 | \n\t\t
Comparison of Means and Standard Deviations of Age and Treatment Period in the Root Resorption and Control Groups
Determination of the root resorption status of each patient was established by comparing the dental panoramic radiographs that had been taken before and after treatment. All teeth were measured along the tooth’s longitudinal axis for root length (defined as cement-enamel junction to apex of root). This method was similar to that used by Linge & Linge [7]. Individuals were assigned to the root resorption group when it was disclosed that one or more of their dental roots had been shortened by more than 25% of the original root length in the time that had elapsed between the two radiographs. The root shape was recorded subjectively as normal or abnormal (shortened, blunt, eroded, pointed, bent, bottle shaped) (Fig. 3) by examining dental panoramic radiographs that had been taken before the onset of treatment.
Morphology classification of the root (A) Normal, (B) Shortened, (C) Pointed, (D) Blunt, (E) Eroded, (F) Bent, (G) Bottle shaped.
The questionnaire sought information on the following subjects: personal details, medical history, dental history, and oral habits. Information on medical history included local or systemic diseases, details on medication uptake, hospitalizations, allergies, health during gestation and first year of life, childhood diseases, other conditions, and developmental events in infancy and childhood. Dental history questions referred to details on previous dental treatment and information about past oral injuries. Habits listed in the questionnaire were nail biting, tongue thrusting, and mouth breathing.
The validity of our hypothesis was tested by the logistic regression analysis using the Stat View 5.0 pro- gram (SAS Institute Inc, Cary, NC). Logistic regression produces odds ratios associated with each predictor variables (root anomaly, extraction, trauma, gingivitis, allergy, asthma, systemic disease, medication use, thumb sucking, nail biting, tongue thrusting, mouth breathing).
The prevalence of excessive root resorption was 7.8%. The distribution of each risk factor in the root resorption and the control groups is shown in Figure 4. The logistic regression analysis is shown in Table 2. The incidence of allergy and asthma was significantly higher in the root resorption group (P = 0.005), with a mean odds ratio of 2.54 and 95% confidence interval of 1.34–4.92. The incidence of root anomaly was significantly higher in the root resorption group (P = 0.001), with a mean odds ratio of 2.95 and 95% confidence interval of 1.53–5.83.
Prevalence of each risk factor in the root resorption and control groups.
\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t
Root anomaly | \n\t\t\t0.001 | \n\t\t\t2.95 | \n\t\t\t1.53-5.83 | \n\t\t
Extraction | \n\t\t\t0.168 | \n\t\t\t1.56 | \n\t\t\t0.83-2.98 | \n\t\t
Trauma | \n\t\t\t0.473 | \n\t\t\t1.59 | \n\t\t\t0.45-5.97 | \n\t\t
Gingivitis | \n\t\t\t0.120 | \n\t\t\t0.62 | \n\t\t\t0.34-1.13 | \n\t\t
Allergy | \n\t\t\t0.005 | \n\t\t\t2.54 | \n\t\t\t1.34-4.92 | \n\t\t
Systemic disease | \n\t\t\t0.900 | \n\t\t\t0.93 | \n\t\t\t0.32-2.70 | \n\t\t
Medication use | \n\t\t\t0.344 | \n\t\t\t2.31 | \n\t\t\t0.45-17.2 | \n\t\t
Nail biting | \n\t\t\t0.311 | \n\t\t\t1.57 | \n\t\t\t0.66-3.84 | \n\t\t
Mouth breathing | \n\t\t\t0.539 | \n\t\t\t0.76 | \n\t\t\t0.31-1.81 | \n\t\t
Logistic Regression Analysis of Each Risk Factor
This clinical study showed that allergy might be an aetiological factor in increased root resorption. The same association was found in earlier studies [1,57]. However, those studies were primarily performed on Caucasian subjects. Our finding, derived from an examination of the clinical records of Japanese subjects, supports the hypothesis that allergy is a high risk factor for the development of excessive root resorption during orthodontic treatment.
Our results also indicate that abnormal root shape is probably associated with excessive root resorption (p=0.056). This finding is in agreement with the results of the previous study [23,58-60]. If the same orthodontic force is applied to the dental crown, the root apex is exposed to increasing stress as the root becomes shorter. Additionally, when the same orthodontic force is applied to the root apex, the distribution of the stress is different according to the types of root anatomy, and the dental root with pointed or bent shape may be exposed to larger stress than roots with normal morphological features. These increased stresses may traumatize the apical periodontal ligament, followed by an inflammatory/repair process, which includes resorption of the root apex.
Patients with periodontal disease have circulating primed monocytes, and sera of patients with periodontal disease contain high levels of proinflammatory cytokines [8]. In this study, however, gingivitis did not have a significant association to orthodontic root resorption. This is most likely due to the sample selection of young patients; mean age of 17.8 ± 6.2 years, and the method used to detect the status of periodontal health; examination of intra-oral photographs.
In cases requiring tooth extraction, the remaining teeth are usually moved relatively great distance, particularly when maxillary incisors are retracted in order to reduce large overjet [58,59,61]. Additionally, biologically, tooth extraction and the ensuing wound healing attract vast numbers of immune cells to the extraction site. These inflammatory cells may directly spread from the wound site to tissues surrounding adjacent teeth or, indirectly, produce large amounts of cytokines that enter the circulation and exit into the extravascular space in the periodontal ligament of neighboring mechanically stressed teeth, which regulate remodeling activities not only at extraction site but also in tissues surrounding adjacent teeth. However, in this study, we have not found a significant association between extraction of permanent teeth and orthodontic root resorption. Therefore, we conclude that healing of extraction sites is primarily a local event which does not promote the resorption of adjacent teeth.
One limitation of this study was using the panoramic radiographs in order to evaluate the amount of root resorption. Although this method of examination makes it possible to view the whole dentition, its main drawback is distortion of the tooth image, predominantly in the incisor region. Periapical radiographs would be preferable to determine the size and shape of dental roots, but these radiographs were not available to us this time.
In the experimental animal study, we used Brown–Norway (BN) rats, which are known to produce high levels of IgE after sensitization with OVA. BN rats have been used extensively as animal models of allergic asthma [62–64], atopic dermatitis [65], and food allergies [66,67].
This study aimed to determine whether systemic allergic inflammation had adverse effects on orthodontic tooth movement and bone and root resorption and, if so, to investigate the possible mechanisms of the acceleration. Furthermore, we examined the ability of aspirin, which has been reported to improve bone mineral density (BMD) in human epidemiological studies [68,69], to reverse tooth root resorption.
Six-week-old male BN rats (weighing 110–140 g) were sensitized by a subcutaneous injection of saline (1 mL) containing 1 mg of OVA (grade V; Sigma-Aldrich, St. Louis, MO, USA) and 200 mg of aluminum hydroxide. A
Maxillary first molar (M1) was moved medially with Ni-Ti Coil spring (0.1N) (Murata et al., 2013).
The animals were divided into four groups: OVA with OF group, OVA alone group (no OF), OF alone group (no OVA), and control group (no OVA or OF).
After 7 or 14 days of OF (at days 7 and 14, respectively), the animals were perfused transcardially. The two halves of the maxilla were decalcified in 10% ethylenediamine-tetraacetic acid and cut into 8-μm-thick parasagittal sections. The sections were stained for tartrate-resistant acid phosphatase (TRAP) (Sigma-Aldrich) and counterstained with toluidine blue.
We measured the area of ERR as well as the number of odontoclasts and osteoclasts within a defined area according to the method of Mavragani et al. [70]. TRAP-positive cells in resorption lacunae that faced the tooth roots were counted as odontoclasts, and other TRAP-positive cells (containing more than two nuclei) were counted as osteoclasts.
Proinflammatory cytokines were measured in the periodontal tissues, including the bone surrounding M1, after 24 hours of OF. The levels of TNF-α, IL-1β, and IL-6 were evaluated using a TNF-αELISA Kit (Shibayagi), IL-1β ELISA Kit, and IL-6 ELISA Kit (Biosource, Camarillo, CA, USA), respectively, according to the manufacturers’ protocols.
The lipid components were extracted in 100% methanol at –30°C overnight.
The periodontal tissues around M1 after 24 hours of OF were rapidly frozen in liquid nitrogen and homogenized. After mRNA isolation, quantitative real-time PCR or conventional RT-PCR was performed with gene-specific primer sets as described previously [71].
Micro-CT images were acquired at a resolution of 9 µm using a SkyScan 1076 (SkyScan, Antwerp, Belgium) to assess the degree of OF. At day 14 (10 weeks of age), the hemi-maxilla samples were excised. The hemi-maxilla samples were directed parallel to the occlusal plane and scanned for 2.70 mm. The distance from the distal surface of M1 to the mesial surface of the second molar (M2) was measured using DataViewer software (ver. 1.4.3; SkyScan).
Aspirin dissolved in feed water (250 μg/kg/day) was administered orally to OVA-sensitized rats with orthodontic force application. For histologic experiments, aspirin was administered from the initiation of OF. For ELISA and RT-PCR analyses, aspirin was administered from 24 hours before OF.
The total serum IgE was compared using Student’s
At day 14, the degree of tooth movement in the OVA with OF group was significantly larger than that in the OF alone group. Without orthodontic force application, no osteoclasts and/or odontoclasts were observed on the medial surface of the alveolar bone in any of the groups (Fig. 6A, 6B). On day 7, marked ERR was observed on the pressured side in the OF alone group, especially in the OVA with OF group (Figs. 7A, 7B). On day 14, these tendencies became further significant in both the OF alone and the OVA with OF groups (Figs. 8A, 8B). The OVA with OF group exhibited significantly broader and deeper TRAP-stained areas compared to the OF alone group on days 7 as well as 14 (Fig. 9A). The number of odontoclasts and osteoclasts was significantly greater in the OVA with OF group than in the OF alone group on day 7 (Figs. 9B, 9C). The number of osteoclasts peaked on day 7 in the OVA with OF group and then decreased by day 14. While the area of ERR increased from day 7 to day 14, the number of odontoclasts was maintained through days 7 to 14 (Figs. 9A, 9C). The alveolar bone resorption and osteoclast number were significantly greater in the OVA with OF group than in the OF alone group on days 7 and 14 (Figs. 9D, 9E).
Histologic sections of periodontal tissues around the distopalatal root of the first molar stained for TRAP. (A) Control group (no treatment), (B) OVA-alone group (Murata et al., 2013).
Histologic sections of periodontal tissues around the distopalatal root of the first molar stained for TRAP on day 7. (A) OVA with OTM group, (B) OTM-alone group (Murata et al., 2013).
Histologic sections of periodontal tissues around the distopalatal root of the first molar stained for TRAP on day 14. (A) OVA with OTM group, (B) OTM-alone group (Murata et al., 2013).
The numbers of osteoclasts/odontoclasts and amounts of root/bone resorption on the pressured side after tooth movement. (A) Area of root resorption (×103 μm2), (B) Number of osteoclasts, (C) Number of odontoclasts, (D) Eroded surface per alveolar bone surface (ES/ BS), (E) Number of osteoclasts per alveolar bone surface (N.Oc/BS). *:
The expression levels of RANKL, TNF-α, and IL-1β were significantly higher in the OVA with OF group than in the other groups after 24 hours of OF (Figs. 10A–10C), while the difference in IL-6 was not significant (Fig. 10D). Although no significant differences were observed, the OF alone group showed tendencies toward higher levels of TNF-α and IL-1β (Figs. 10B, 10C).
Concentrations of RANKL and inflammatory cytokines in rat periodontal tissues with or without OVA sensitization, after 24 h of orthodontic force application, and after aspirin administration. (A) RANKL, (B) TNF-α, (C) IL-1β, (D) IL-6. *:
The LTB4 level and mRNA levels of the leukotriene synthases LTA4h were significantly increased in the OVA with orthodontic force group after 24 hours of orthodontic force compared with the other groups (Figs. 11A, 11B).
Effects of OVA sensitization, OF application for 24 h, and aspirin administration on the leukotriene synthases. (A) LTB4, (B) LTA4h. *:
Aspirin administration reversed the degree of tooth movement in the OVA with OF group to a similar level of the OF alone group on day 14. Histologic examinations revealed that the area of ERR and the numbers of odontoclasts and osteoclasts were decreased to the levels observed in the OF alone group (Figs. 9A–9E). The expression levels of RANKL mRNA and TNF-α, IL-1β, and IL-6 proteins were also suppressed in the levels detected in the OF alone group (Figs. 10A–10D). Furthermore, the expression levels of LTB4 and LTA4h were suppressed by aspirin treatment (Fig. 11).
We demonstrated that allergies affect ERR during orthodontic treatment using an animal model. It is notable that the degree of OF in the present study was greater in the OVA-sensitized animals, suggesting that allergies can affect OF. In the histological observations, we found that the OVA-sensitized rats showed increased numbers of odontoclasts and osteoclasts on the pressured side during OF. The present findings suggested that allergies enhanced both odontoclastogenesis and osteoclastogenesis under the condition of orthodontic force.
TNF-α, IL-1β, and IL-6, which are well-known proinflammatory cytokines, were detected at high levels in the periodontal tissues from the OVA with OF group. Recently, Polzer et al. [72] suggested that IL-1β contributes to TNF-mediated inflammatory osteopenia, reporting that TNF-α transgenic IL-1β-deleted mice were protected against bone loss. The elevated expression levels of TNF-α and IL-1β, together with RANKL as an essential cytokine for osteoclastogenesis, suggested boosts in the rates and magnitudes of ERR as well as orthodontic tooth movement due to the differentiation of osteoclasts and odontoclasts.
Lipid mediators derived from arachidonic acid through the lipoxygenase and cyclooxygenase pathways are known to act as important modulators of inflammation. Increased levels of leukotrienes are found in inflammatory diseases such as asthma, arthritis, and periodontal disease [73-75]. LTB4 has been reported to stimulate osteoclast formation and bone resorption in the mouse calvaria [55] and to inhibit osteogenesis [76]. In our model, the expression levels of the leukotriene synthases LTB4 and LTA4h were increased in the periodontal tissues in OVA-sensitized rats with OF. The fact that mechanical loading with OVA sensitization elevated the expression of leukotriene synthases reveals a potential role for leukotrienes in bone and root resorption. Interestingly, these effects of mechanical OF suggest the involvement of the lipoxygenase pathway in response to mechanical loading. We assume that the present findings are compatible with our previous epidemiologic observations [33,34].
We further found that oral administration of aspirin was able to reverse the increases in ERR and degree of OF in the allergic model. The dose of aspirin in the present study, 250 μg/kg/day, was much lower than that for general use as a painkiller. A recent study showed that low-dose aspirin inhibited ovariectomy-induced osteoporosis by inhibiting T cell activation [77]. Our findings raise the possibility that low-dose aspirin administration inhibits osteoclastogenesis under inflammatory conditions.
Through the epidemiological and animal model studies, we have demonstrated that allergies would be possible risk factors of ERR in the course of orthodontic treatments. In the clinical study, the evidence would be strengthened using a prospective study involving a much larger sample, such as the randomized controlled test. Moreover, we need to investigate the unsolved mechanism of ERR, and the elucidation of the pharmacological targets for ERR during orthodontic tooth movement would be warranted.
In the clinical study, we found that allergy and root morphology abnormalities may be considered high-risk factors for the development of excessive root resorption during the course of orthodontic treatment.
We have proposed a model for studying the effects of allergen-induced inflammation and/or mechanical stress on ERR during OTM. This process was affected by pro-inflammatory cytokines, together with lipid mediators. Furthermore, our findings suggest that pro-inflammatory cytokines and leukotrienes, together with low-dose aspirin, may represent pharmacological targets for ERR during orthodontic treatment with allergic conditions.
This work was supported by grants-in-aid for Scientific Research (C) (no. 18592238 to H.I), Scientific Research (C) (no. 21592600 to H.I), and Scientific Research (C) (no. 24593095 to H.I) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We would like to express our sincere gratitude to Professor Ze’ev Davidovitch and Professor Amy L. Counts for continuing support to our research.
Effluents of industrial wastes are the important source of water pollution that are toxic in the environment and are becoming the biggest problem that influence the survival of human beings and other creatures in the environment. The demand of clean water is increasing with the rapid growth of global population; in the present, several treatment techniques that include biodegradation, membrane process, coagulation, adsorption, precipitation, sonochemical degradation, micellar-enhanced ultrafiltration, and advanced oxidation process (AOP) have been utilized to remove pollutants from wastewater [1]. Among them, AOP is an efficient method to remove the contaminants that are not degradable by means of biological processes. Since these processes involve the production of very reactive oxygen species (ROS), it can be able to destroy a wide range of organic compounds. A wide range of semiconductor photocatalysts have been utilized in AOPs. The first photocatalyst, TiO2, discovered by Fujima and Honda in 1972 was found as gold standard due to its significant characteristics that include high chemical stability, nontoxicity, and relatively low price [2]. However, it only absorbs ultraviolet irradiation from sunlight due to its wide bandgap. To overcome this problem, several studies are focusing on finding ways to extend the absorption wavelength range of TiO2. In this regard, metal or nonmetal elements doped onto TiO2 and visible light response photocatalysts are being developed as the future generation. Among which transition metal chalcogenide (TMC) materials gained worldwide attention in recent decades because of their significant characteristics, such as excellent optical absorption due to its tunable indirect bandgap energies (1–2 eV) [3]. Moreover TMCs with graphene oxides [4], carbon nitride [5], metal oxides [6], and metals [7] were found to increase the conductivity of electrons, provide active sites, and effectively separate the electron and hole pairs generated by the semiconductor photocatalysts [4]. This chapter explores the synthesis, characterization, and the applications of TMCs.
\nTransition metal chalcogenides are considered as emerging candidates due to their unique physical and chemical properties and are being researched for use in lithium-ion batteries, solar cells, hydrogen evolution, and photocatalytic degradation due to their indirect bandgaps, optoelectronic behavior, and stability. In addition, nanodots (quantum dots)/nanostructures of these metal chalcogenides show stronger edge effects, and the quantum confinement effect makes it possible to utilize under solar-simulated irradiation [8, 9].
\nThere are several methods to synthesize transitional metal chalcogenides, including ultrasonic chemical method, hydrothermal method, simple template-free one-pot method, an ion-exchange and precipitation methods, simple microwave-assisted solvothermal process, and surface modification method [5, 6, 7, 8, 9, 10, 11, 12]. The following sections explore the most common preparation methods of TMCs.
\nHydrothermal reaction usually occurs in the reaction vessel of stainless steel autoclave. The presence of aqueous solution or mineralizers under high pressure and low temperature encourages interaction of precursor materials during the process. This method has the feature to make high crystalline nanostructures or products at relatively low temperature [13, 14]. A range of TMCs that include NiS [15], CuS, FeS2, NiS2 [10], etc. have been synthesized using hydrothermal treatment method due to low temperature requirement (150°C), short time duration (~4 h), and high yield (>90%). In addition, there is no need to use any complexing agents or no need to control the pH [10] (Figure 1).
\nSchematic illustration of the hydrothermal synthesis of FeS2, CuS, and NiS2 powders. Reproduced with permission from [
Synthesis of CdS nanosheet-RGO hybrid. Reproduced with permission from [
Further, hydrothermal method has been utilized to synthesize TMC-doped metal [16], metal oxides [17], or carbonaceous [18] materials. The following schematic illustration represents the preparation of CdS nanosheet-RGO hybrid material using hydrothermal treatment method (Figure 2).
\nApart from these, microwave-assisted synthesis method has also been used to prepare TMCs with controllable size and shape compared with conventional heating method due to the homogeneous heating process. Further, this method can promote nucleation and, thus, reduce the time required for synthesis [18].
\nSimple template-free one-pot method is a simple synthetic approach that was used to develop certain metal oxide hollow spheres based on direct solid evacuation arising from Ostwald ripening, the Kirkendall effect, or chemically induced self-transformation [19].
\nFigure 3 illustrates the mechanism for the simple template-free one-pot method to prepare hollow sphere, Ni-doped CdS material. Herein, biomolecular glutathione was used as sulfur and bubble source [7].
\nMechanism for the formation of Ni-doped CdS. Reproduced with permission from [
The essential techniques used to characterize the transition metal chalcogenide materials are discussed in this section.
\nX-ray diffraction (XRD) spectroscopy is a nondestructive method used to analyze the crystallinity, crystal structure, crystallite size, and phase composition of the photocatalytic materials which presents in powder form or thin film.
\nXRD pattern for MoS2 on g-C3N4 in different amounts. Reproduced with permission from [
In the study by Li and co-workers, MoS2 nanosheets coupled into the carbon nitride to form MoS2/C3N4 heterostructures synthesized by a facile ultrasonic chemical method were characterized by using XRD. All the samples prepared showed similar patterns, and the peaks at 32.6 and 58.3° were assigned to the (100) and (110) crystal plane formation of MoS2. Peak at 27.4° indicated to the (002) stacking layered structure, while the 13.0° peak corresponds to the (100) in-plane repeated units. The observation from XRD confirms the formation of MoS2/C3N4 heterostrucuture [5] (Figure 4).
\nIn another study, NiS nanoparticles prepared hydrothermally were characterized by powder XRD method.
\nFigure 5 illustrates the XRD patterns of as-prepared nanostructured NiS samples. The patterns were indexed to rhombohedral structured NiS with the space group of R3m. The cell parameters were found to be a = 9.61 Å and c = 3.16 Å [15].
\nXRD for synthesized NiS. Reproduced with permission from [
XRD pattern (Figure 6) confirmed the formation of CdS, and Ni-doped CdS materials prepared by one-pot synthesis method show similar pattern and are assigned to hexagonal phase. It was also found that the peak intensity increases with the increase in Ni2+ doping concentration. Average crystallite size was estimated based on the broadening of the (002) peak, and it was found that the crystallite size also increased with the %Ni doped on CdS [7].
\nXRD for Ni doped on CdS. Reproduced with permission from [
X-ray powder diffraction patterns of different metal sulfides were reported by Ali and co-workers as illustrated in Figure 7. The diffraction patterns of the samples are indexed to pyrite FeS2, covellite CuS, and vaesite NiS2. The purity of the phases was confirmed by the absence of additional peaks in their XRD. XRD patterns of cubic FeS2 and NiS2 were found to be isostructural, whereas CuS was found in a hexagonal symmetry. The crystallite sizes of the materials were estimated as 50, 45, and 22 nm for FeS2, CuS, and NiS2, respectively [10].
\nXRD diffraction patterns and schematic representations of the crystalline structure of (a) FeS2, (b) CuS, and (c) NiS2. Reproduced with permission from [
Scanning electron microscopy (SEM) is one of the electron microscopic techniques that images the sample surface using high beam electrons. In this technique, beam electrons strike the surface of the specimen and interact with atoms and form the secondary electrons, which characterized the information about the morphology of sample surface in high-resolution images colored in black and white. SEM is used to view dispersion of nanoparticles in different nanostructures, such as nanotubes, nanoclays, nanofillers, and nanofibers. In addition, the method used X-ray to identify elemental composition known as energy-dispersive ray method (EDX). The transmission electron microscopy (TEM) images form when beam of electron is transmitted through the sample and magnified by objective lens. This technique is the most preferred to measure the particle size and thickness of sample. In particular, for the morphology of materials, monitoring morphology and dispersion is very crucial compared to scanning electron microscopy [20].
\nFigure 8(a) shows SEM images of carnation flowerlike morphology with a diameter of about 500–700 nm. Figure 8(b)
(a–c) SEM images of SnS2 flowerlike structure, (d–e) TEM images of SnS2 flowerlike structure, and (f) SEM images of SnS2 nanoparticles. Reproduced with permission from [
SEM and EDS analysis of (a, b) FeS2, (c, d) CuS, and (e, f) NiS2. Reproduced with permission from [
In the green synthesis of earth-abundant metal sulfides by Ali and co-workers, the samples prepared exhibited uniform morphology and particle size distribution, which is due to a controlled growth during the hydrothermal synthesis. The presence of iron, copper, nickel, sulfur, and carbon and its purity obtained using SEM analysis was found to be in good agreement with the phases observed by XRD. In addition, the atomic content of Fe, Cu, Ni, and S in the samples had a good correlation with theoretical atomic% of all the phases. The morphological analysis confirmed that as-prepared sulfides are in the form of agglomerated particles. In addition, polyhedral particles with the size of ~676 ± 44 nm were obtained for FeS2 sample, whereas CuS and NiS2 exhibited irregular- and spherical-shaped particles with the size of ~783 ± 53 and 933 ± 68 nm, respectively [10] (Figure 9).
\nLi et al. studied the morphology and microstructure of the as-prepared samples of WS2 by using SEM and TEM. The detailed structural information of the WS2/Bi2MoO6 composite was obtained by TEM analysis, and an irregular platelet-shaped nanostructure was observed with the length ranging from 500 nm to 2 μm. The results obtained from TEM were in good agreement with SEM analysis. The HRTEM of the prepared materials further confirm the nanojunction structure. Figure 10 illustrates two sets of different lattice images with the lattice fringe of 0.27 and 0.46 nm obtained from TEM corresponded to the (100) plane of WS2 and (120) plane of Bi2MoO6 resulted from the XRD [17].
\nTEM and HRTEM images of the hierarchical WS2/Bi2MoO6 composite (5 wt% of WS2). Reproduced with permission from [
XPS or X-ray photoelectron spectroscopy is used as surface analytic technique based on the photoelectric effect. XPS is mainly used to find the composition of elements in the surface of the materials and to determine the valence band structure and chemical state of components.
\nFigure 11(a) illustrates the XPS molybdenum 3d spectra for MoSe2, which exhibit peaks on 228.84 and 232.01 eV indicating binding energy of Mo 3d5/2 and Mo 3d3/2 for Mo4+ ions, respectively. In addition, Figure 11(c) depicts the XPS of MoSeX (at 120°C) indicating additional peaks at 232.07 and 229.45 eV which can be attributed to the binding energy of Mo5+ ions. The results confirmed the synthesis at 120°C, which resulted in two chemical states (IV and V) of molybdenum. Further, it was found that the amorphous MoSe2 showed a very broad Se 3d peak than the crystalline MoSe2. The Se 3d5/2 and Se 3d3/2 peaks for amorphous and crystal samples of MoSe2 were found to lie between the range of 52 and 57 eV; however, the intensity of the peaks varied in amorphous sample. Peak-fitting analysis showed that the ratio of Mo to Se in the amorphous MoSex sample was 1:3:1 [3].
\n(a, b) XPS for MoSe2, (c, d) XPS for MoSeX. Reproduced with permission from [
Figure 12(a) shows the schematic of PtSe2 in monolayer format, in that one sublayer of Pt atoms is sandwiched between two Se sublayers. Figure 12(b) shows the XPS measurements for Se during the growth of PtSe2 at 270°C. The peaks at 55.68 and 54.80 eV indicate the binding energy of Se(0) chemical state at 25°C, and at 270°C, the peaks were found at 55.19 and 54.39 eV corresponding to the binding energy of Se2− chemical state; in between that, at 200°C, the curve indicates the coexistence of Se(0) and Se2− states. The binding energies of Se confirmed the formation of PtSe2 at 270°C [22].
\n(a) PtSe2 monolayer and (b) XPS for PtSe2. Reproduced with permission from [
The XPS survey spectrum of SnS2-AP (Figure 13) confirmed the presence of Sn and S components, whereas Cr, C, and O were found to be the contaminants. High-resolution XPS spectra of Sn 3d and S 2p core levels proved that the binding energies of Sn 3d and S 2p of SnS2-AP were very close to those of SnS2-(c). The binding energies of Sn 3d5/2 and S 2p3/2 of SnS2-AP and SnS2-(c) were 486.61 and 486.65, 161.68, and 161.74 eV, respectively. It was also found that the binding energies of Sn 3d5/2 and S 2p3/2 of SnS2-AP and SnS2-(c) were of close proximity with the reference data of Sn4+ and S2− in SnS2.
\nXPS spectra of SnS2-AP and SnS2-(c). Reproduced with permission from [
Apart from these, a peak attained at 577.36 eV is due to the binding energy of Cr 2p3/2 corresponding to Cr (III) in Cr(OH)3. The hydrolysis-precipitation of Cr(III) ion leads to the formation of Cr(OH)3 on the surface of SnS2-AP. Cr(III) were generated from the photocatalytic reduction of adsorbed Cr(VI) [23].
\nPersistent organic pollutants (POPs) are of global concern because of their potential for long-range transport, persistence in the environment, ability to biomagnify and bioaccumulate in ecosystems, as well as possess negative effects on human health and the environment. In particular, the agrochemicals and textile effluents involve processes that produce compounds that are very toxic to the environment. Thus, it is necessary to remove these colored pollutants from the environment. Several catalysts have been utilized to remove the pollutants from the polluted environment, and the transition metal chalcogenide has recently gained much attention in this regard due to their significant characteristic properties.
\nRepeated tests using recycled CdS 0.03(MoS2/0.01rGO) composite catalyst for photocatalytic 4-nitrophenol reduction. Reproduced with permission from [
Wen-chao Peng et al. synthesized MoS2/reduced graphene oxide hybrid with CdS nanoparticle for photocatalytic reduction of nitroaromatic compounds to aromatic amines under visible light irradiation in the presence of sacrificial agent. It was also noted that composite of CdS-0.03 (MoS2/0.01rGO) exhibited remarkable enhancement on the 4-nitrophenol reduction due to the separation of electron-hole pairs generated by CdS nanoparticles [4] (Figure 14). In addition, it was found that the photocatalyst appeared to be stable confirmed by recycling study as illustrated in the following figure.
\nIn another study, Peitao Liu and co-workers worked on prepared N-doped MoS2 nanoflowers (specific surface area of 114.2 m2 g−1) and α-Fe2O3@N-doped MoS2 heterostructures for decoloring or the removal of the Rhodamine B (RhB) under visible light irradiation. When comparing the activity of these two materials, N-doped MoS2 nanoflowers showed optimum rate constant as 0.0928 min−1, which was 26.4 times greater than that of MoS2 nanosheets. Further, 20 mg of these catalysts completely degraded the 50 mL of 30 mgL−1 RhB within 70 min [24] (Figure 15).
\nPhotocatalytic degradation of RhB by different photocatalysts (N-doped MoS2 nanoflower, without light, bulk N-doped MoS2, and MoS2 nanosheets) under visible light irradiation, where C and C0 are the RhB concentrations at time t min. And 0 min, respectively. Reproduced with permission from [
MoS2 nanosheets were coated into carbon nitride synthesized by facile ultrasonic chemical method to form heterostructures. These materials were used to explore the photocatalytic degradation of the dyes, RhB and MO, and the optimum reaction rate constant was found to be 301 min−1 with the 0.05 wt% MoS2 [5].
\nYangyang Liu and co-workers synthesized ZnO nanosheet doped with P by using conventional chemical vapor transport and condensation (CVTC) method and then coated with an atomic layer of MoS2 on it (Figure 16).
\nMechanism of photolysis on ZnO decorated with MoS2. Reproduced with permission from [
The photocatalytic activity of the catalyst was tested by degradation of organic dyes, such as methylene blue (MB) and Rhodamine B (RhB), under natural sunlight. It was found that 95% of organic dyes were degraded within 6 min and photocatalytic rate constant was found to be 1.413 min−1, which was 3.4 times better than that of P25 due to the enhanced light adsorption efficiency obtained by synergetic effect of MoS2 [6].
\nSchematic illustration of photo-charge separation through 1 T@2H-MoS2/Ag under visible light irradiation. Reproduced with permission from [
In another study, Haiyang Liu et al. worked on 1 T@2H-MoS2/Ag composite synthesized by microwave-hydrothermal and photoreduction methods for photocatalytic degradation of conventional dyes and photocatalytic reduction of Cr(VI) under visible light irradiation. It was observed that 1 T@2H-MoS2/Ag effectively enhanced photocatalytic activity compared with 2H-MoS2 due to the enhancement in the light response range and charge separation by using Ag quantum dots and 1 T phase. In addition, 1 T@2H-MoS2 showed 81 and 41% of increment in photocatalytic reduction of Cr(VI) and photocatalytic degradation of methylene blue, respectively [16] (Figure 17).
\nIn a different study, Xi Yang et al. worked on amorphous and crystalline molybdenum selenide synthesized by facile low-temperature hydrothermal method for adsorption and degradation of Rhodamine and methylene blue under dark and visible light irradiation. Due to the excellent optical absorption and narrow bandgap (1–2 eV), molybdenum selenide (MoSe2) is preferred for the photocatalytic degradation of pollutants. Hole and free radical studies concluded that the photocatalytic effect of the amorphous samples was higher than that of the crystalline samples due to the different active substances. The active substances, superoxide radicals, in particular, predominated the process of degrading the dye. When comparing the amorphous and crystalline materials, amorphous molybdenum selenide showed better performance in adsorption and degradation process due to the formation of superoxide radical (˙O2−) and more unsaturated atoms and greater specific surface area than crystalline structure; further, the holes and hydroxyl radicals were found to be the main active substances [3].
\nHongxu Guo et al. synthesized NiS nanoparticles for photocatalytic degradation and adsorption of Congo red under visible light irradiation. NiS nanoparticles were synthesized by hydrothermal method, and it exhibited efficient photocatalytic activity. 30 mg/L of Congo red was completely degraded after illumination of 210 min. In addition, OH radicals were identified by using fluorescence technique, and those highly reactive radicals were found to be the major factor for the photocatalytic process [15].
\nIn a different study, Aniruddha Molla et al. synthesized Ag-In-Ni-S nanocomposites with different shapes for the degradation of methylene blue under dark and visible light irradiation. It is noteworthy to mention here that 20 mg of the catalyst showed a complete degradation of methylene blue within 12 min, when there was no light exposure. Notably, the degradation took only 2 min, when visible light was exposed with a 100 W lamp source. Further, the reactive oxygen species generated during the process was confirmed in their study [2].
\nCdS and Ni-doped CdS hollow spheres were used by Man Luo and his group for the degradation or removal of RhB and phenol. These materials were synthesized by simple template-free one-pot method. 1.2 mol% of Ni-doped CdS hollow spheres exhibited better performance in the removal of organic pollutants due to the reduce recombination rate of the electrons and hole pairs [7]. Figure 18 illustrates the UV-absorbance profile for the degradation of RhB dye with the 1.2 mol% of Ni-doped CdS.
\nUV-visible absorption spectra of RhB in the presence of 1.2 Mol% of Ni-doped CdS. Reproduced with permission from [
Earth-abundant transition metal sulfides, such as FeS2, CuS, and NiS2, were synthesized by fast and low-cost hydrothermal synthesis method and utilized for photocatalytic hydrogen evolution and photocatalytic degradation of indigo carmine dye under visible light irradiation. Different sacrificial agents, such as Na2S/Na2SO3, EDTA, and ethanol, were used when the H2 evolution was tested. FeS2 showed higher activity for the hydrogen evolution (32 μmolg−1 h−1), and an 88% of dye (indigo carmine) degradation was attained due to their suitable electronic and optical properties. The better activity was credited to a larger crystallite size, smaller particle size, and lower recombination rate of FeS2 than other materials that include CuS and NiS2. In addition, the presence of Na2S/Na2SO3 exhibited higher enhancement in the activity for the production of hydrogen among the other scavenging reagents used in this study [10].
\nIn another study, Yongje Zhao and co-workers utilized the bimetallic chalcogenides material, FeCoS2, synthesized by using a modulated hydrothermal method for photodegradation of methylene blue and anode materials for Na-ion batteries under UV radiation. The photocatalytic activity of FeCoS2 which was hydrothermally treated at 190°C was found to be a better candidate with the degradation efficiency of 90% (in 150 min) under UV irradiation. The better photocatalytic activity was related with the microstructure, specific surface area, and charge separation of catalytic materials [25].
\nAlireza et al. prepared NiS-clinoptilolite zeolite as a catalyst via an ion-exchange and precipitation methods. The catalyst was utilized for photodegradation of furfural in aqueous solution over UV irradiation. An optimum photocatalytic efficiency was observed when 330 mg L−1 of the catalyst was used at pH 5 in 6 mM of furfural solution. In addition, it was found that the degradation rate increased with increasing amount of hydrogen peroxide and potassium bromate in the solution [12].
\nXi Li et al. used SnS2 and CdS for photocatalytic degradation of different types of organic dyes, such as methyl orange (MO), Rhodamine B (RhB), Congo red (CR), orange II (OII), malachite green (MG), and methylene blue (MB), under visible light irradiation, and the activity of these two materials were compared. SnS2 showed higher activity in the degradation of MO and lower activity in the degradation of RhB-like organic dyes, when compared to CdS material. In the degradation of azo dyes (dyes containing N〓N double bond), SnS2 followed a reduction mechanism with photoelectrons via the SnIV/SnII transition, whereas an oxidation mechanism was observed with both SnS2 and CdS for photodegradation of organic dyes which do not consist of N〓N double bond. ˙O2− and ˙OH radicals were found to be the major contributors for the oxidation mechanism. Since the reduction of dye molecules was faster than migration of the radicals, SnS2 exhibited much higher activity than the CdS for the dyes containing N〓N double bond. However, SnS2 showed lower efficiency than CdS in the photocatalytic degradation of other organic dyes without N〓N double bond, due to the slow production of reactive oxidative species mentioned above. Additional experiments were carried out in the presence of the scavengers NO, N2, O2, and IPA [26]. Figure 19 depicts the rate of degradation of both catalysts against the azo and non-azo dyes.
\nPlots of normalized concentration (C0/C) of MB vs. different time intervals (a) MO and (b) RhB photocatalytic degradation. Reaction conditions: 20 mg (a) or 50 mg (b) photocatalyst, 50 mL 6.0 × 10−5 Mol/L organic dyes, a 300 W xenon lamp(>420 nm), reaction temperature = 30°C. Reproduced with permission from [
In a different study, 3D carnation flowerlike hexagonal SnS2 nanostructures were synthesized by a simple microwave-assisted solvothermal process for photocatalytic degradation of Rhodamine (RhB) and phenol under visible light irradiation. The 3D carnation flowerlike hexagonal SnS2 nanostructures exhibited enhanced photocatalytic activity compared to SnS2 nanoparticles due to its high BET surface area, high surface-to-volume ratios, and increased light absorbance of hierarchical mesoporous structures [21].
\nYong Cai Zhang et al. synthesized SnS2 nanoflakes from SnCl2.H2O for photocatalytic degradation of methyl orange (MO) under both the visible light and real sunlight irradiation. The SnS2 material synthesized at 200°C exhibited high photocatalytic efficiency than others (i.e., 100% of degradation of MO after 60 min in the first cycle and 86% in the fifth cycle). The reason for the better degradation efficiency correlated with the combined action of several factors that include bandgap (2.21–2.25 eV), size, dispersibility, suspensibility, surface area, crystallinity, crystal defects, and photochemical stability. Almost all the materials showed better efficiency than the P25 material used for the comparison purpose [27].
\nPhotodegradation of Cr(VI) in the presence of SnS2. Reproduced with permission from [
SnS2 nanocrystals with adjustable sizes were synthesized by Yong Cai Zhang and co-workers by utilizing hydrothermal method with the precursor of SnCl4.5H2O. The materials were tested for the reduction of aqueous Cr(VI) under visible light irradiation (Figure 20).
\nIt was found that the reduction of aqueous Cr(VI) depends on their hydrothermal conditions. Particularly, SnS2, synthesized at 150°C for 12 hours, showed highest photocatalytic activity in reducing aqueous Cr(VI) [10]. In a separate study, the same group worked on size-controlled synthesis of SnS2 by using hydrothermal method, and their photocatalytic activity tested against the degradation of methyl orange under visible light irradiation [23].
\nZhenyi Zhang et al. synthesized nanosheets of SnS2 on g-C3N4 as 2D/2D heterojunction photocatalyst for the photocatalytic degradation of organics including dye (RhB) and phenols over visible light irradiation. It was found that the heterosamples exhibited an enhancement in the photocatalytic degradation of organics than pure g-C3N4 and SnS2 nanosheets. In this study, a better efficiency was attained for the catalyst loaded with 5.0 wt% SnS2 (rate constant of ∼0.2 min−1) for the RhB photodegradation [28].
\nAtkin and co-workers utilized two-dimensional tungsten disulfide (WS2) nanoflakes, hybridized with carbon dots prepared using two-step method for photocatalytic degradation of organic dyes. This material shows photocatalytic efficacy for the degradation of dyes. Twelve percent of degradation was obtained against the Congo red dye when 0.24 mg/L photocatalyst was utilized [29].
\nMechanism for the photocatalytic activity of WS2/Bi2MoO6 in the presence of organic pollutants. Reproduced with permission from [
In another study, few layered WS2/Bi2MoO6 heterojunction composites were prepared by Xiang Li et al. using hydrothermal method for photodegradation of organics under visible light irradiation. A higher degradation efficiency was attained by the materials further proved by the percentage removal of 99.5 and 91.7% for Rhodamine B (RhB), 98.9 and 89.8% for ciprofloxacin (CIP), 76.0 and 67.8% for methylene blue (MB), and 69.3 and 58.6% for methimazole (MMI), respectively. The reason for the removal efficiency was correlated with the structure of the materials, which may provide larger contact area for interfacial charge transfer and can shorten the migration distance of charge transfer [17] (Figure 21).
\nHua-Bin Fang and co-workers used WS2 nanosheets on manganese oxide (MnOX) prepared by photo-deposition in MnSO4 solution. Here, manganese oxide (MnOX) was used as a hole-trapping material. MnOX/WS2 showed better performance in the degradation of RhB under visible light than pure WS2, because of the efficient charge separation in the composite promoted by MnOx [30].
\nPrabhakar Vattikuti et al. synthesized 1D Bi2S3 nanorod/2D e-WS2 nanosheet heterojunction by hydrothermal method for the degradation of methyl orange (MO) dye in aqueous solution and evolution of hydrogen under visible light irradiation. The material showed enhanced photocatalytic activity due to the presence of strong surface active sites and fast transfer of electron–hole pairs in the heterostructure of the composite. In addition, 84.4% of methyl orange was degraded after 90 min of exposure of visible light irradiation when 3% of Bi2S3/e-WS2 composite was used [31].
\nRajesh Bera et al. synthesized CdS nanosheet (0D, 1D, and 2D) on graphene oxide composite by surface modification method. The catalysts prepared were utilized for the photocatalytic degradation of 4-aminothiophenol under visible light. The photocatalytic activity of CdS nanosheet/RGO composite was found to nearly 4 times, 3.4 times, and 2.5 times higher than CdS nanoparticle/RGO, CdS nanorod/RGO, and pure CdS nanosheet samples, respectively. In addition, 2D-2D nanoarchitecture was found to more effective than 0D-2D and 1D-2D hybrid systems due to its better ability for harvesting of photon from sunlight and transport of excitons to their reaction sites than others [18].
\nSohrabnezhad et al. worked on CoS nanoparticles supported on Al-MCM-41 material, which was synthesized by ion-exchange method. The materials were utilized for photocatalytic degradation of basic blue 9 or methylene blue (MB). Seven percent loading of CoS on the mesoporous support exhibited maximum efficiency for the photocatalytic degradation of methylene blue, and the optimum pH was found to be 9. The degradation followed pseudo-first-order reaction with the rate constant (k) of 0.0361. The effect of pH, amount of photocatalyst, and initial concentration of dye were also examined in their studies [11].
\nMetal sulfides/selenites (metal chalcogenides) have attracted considerable interest due to their electronic and optical applications. In addition, most of the common metal sulfides are nontoxic with narrow bandgap. Due to these characteristic features, metal chalcogenides have been utilized as potential candidates for photocatalysis. This chapter reviewed the most common synthetic protocol of several transition metal chalcogenides that include metal sulfides and metal selenites. Further, a brief section covered the basic characterization techniques, which have been commonly used to characterize the chalcogenide materials. Most importantly, the application of different metal chalcogenides toward environmental remediation applications, i.e., degradation of organic pollutants, is also explored herein.
\nThe authors would like to thank the Department of Chemistry, University of Jaffna, Jaffna, Sri Lanka.
\nThe authors declare no conflict of interests.
"Open access contributes to scientific excellence and integrity. It opens up research results to wider analysis. It allows research results to be reused for new discoveries. And it enables the multi-disciplinary research that is needed to solve global 21st century problems. Open access connects science with society. It allows the public to engage with research. To go behind the headlines. And look at the scientific evidence. And it enables policy makers to draw on innovative solutions to societal challenges".
\n\nCarlos Moedas, the European Commissioner for Research Science and Innovation at the STM Annual Frankfurt Conference, October 2016.
",metaTitle:"About Open Access",metaDescription:"Open access contributes to scientific excellence and integrity. It opens up research results to wider analysis. It allows research results to be reused for new discoveries. And it enables the multi-disciplinary research that is needed to solve global 21st century problems. Open access connects science with society. It allows the public to engage with research. To go behind the headlines. And look at the scientific evidence. And it enables policy makers to draw on innovative solutions to societal challenges.\n\nCarlos Moedas, the European Commissioner for Research Science and Innovation at the STM Annual Frankfurt Conference, October 2016.",metaKeywords:null,canonicalURL:"about-open-access",contentRaw:'[{"type":"htmlEditorComponent","content":"The Open Access publishing movement started in the early 2000s when academic leaders from around the world participated in the formation of the Budapest Initiative. They developed recommendations for an Open Access publishing process, “which has worked for the past decade to provide the public with unrestricted, free access to scholarly research—much of which is publicly funded. Making the research publicly available to everyone—free of charge and without most copyright and licensing restrictions—will accelerate scientific research efforts and allow authors to reach a larger number of readers” (reference: http://www.budapestopenaccessinitiative.org)
\\n\\nIntechOpen’s co-founders, both scientists themselves, created the company while undertaking research in robotics at Vienna University. Their goal was to spread research freely “for scientists, by scientists’ to the rest of the world via the Open Access publishing model. The company soon became a signatory of the Budapest Initiative, which currently has more than 1000 supporting organizations worldwide, ranging from universities to funders.
\\n\\nAt IntechOpen today, we are still as committed to working with organizations and people who care about scientific discovery, to putting the academic needs of the scientific community first, and to providing an Open Access environment where scientists can maximize their contribution to scientific advancement. By opening up access to the world’s scientific research articles and book chapters, we aim to facilitate greater opportunity for collaboration, scientific discovery and progress. We subscribe wholeheartedly to the Open Access definition:
\\n\\n“By “open access” to [peer-reviewed research literature], we mean its free availability on the public internet, permitting any users to read, download, copy, distribute, print, search, or link to the full texts of these articles, crawl them for indexing, pass them as data to software, or use them for any other lawful purpose, without financial, legal, or technical barriers other than those inseparable from gaining access to the internet itself. The only constraint on reproduction and distribution, and the only role for copyright in this domain, should be to give authors control over the integrity of their work and the right to be properly acknowledged and cited” (reference: http://www.budapestopenaccessinitiative.org)
\\n\\nOAI-PMH
\\n\\nAs a firm believer in the wider dissemination of knowledge, IntechOpen supports the Open Access Initiative Protocol for Metadata Harvesting (OAI-PMH Version 2.0). Read more
\\n\\nLicense
\\n\\nBook chapters published in edited volumes are distributed under the Creative Commons Attribution 3.0 Unported License (CC BY 3.0). IntechOpen upholds a very flexible Copyright Policy. There is no copyright transfer to the publisher and Authors retain exclusive copyright to their work. All Monographs/Compacts are distributed under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Read more
\\n\\nPeer Review Policies
\\n\\nAll scientific works are Peer Reviewed prior to publishing. Read more
\\n\\nOA Publishing Fees
\\n\\nThe Open Access publishing model employed by IntechOpen eliminates subscription charges and pay-per-view fees, enabling readers to access research at no cost. In order to sustain operations and keep our publications freely accessible we levy an Open Access Publishing Fee for manuscripts, which helps us cover the costs of editorial work and the production of books. Read more
\\n\\nDigital Archiving Policy
\\n\\nIntechOpen is committed to ensuring the long-term preservation and the availability of all scholarly research we publish. We employ a variety of means to enable us to deliver on our commitments to the scientific community. Apart from preservation by the Croatian National Library (for publications prior to April 18, 2018) and the British Library (for publications after April 18, 2018), our entire catalogue is preserved in the CLOCKSS archive.
\\n\\nOpen Science is transparent and accessible knowledge that is shared and developed through collaborative networks.
\\n\\nOpen Science is about increased rigour, accountability, and reproducibility for research. It is based on the principles of inclusion, fairness, equity, and sharing, and ultimately seeks to change the way research is done, who is involved and how it is valued. It aims to make research more open to participation, review/refutation, improvement and (re)use for the world to benefit.
\\n\\nOpen Science refers to doing traditional science with more transparency involved at various stages, for example by openly sharing code and data. It implies a growing set of practices - within different disciplines - aiming at:
\\n\\nWe aim at improving the quality and availability of scholarly communication by promoting and practicing:
\\n\\n\\n"}]'},components:[{type:"htmlEditorComponent",content:'
The Open Access publishing movement started in the early 2000s when academic leaders from around the world participated in the formation of the Budapest Initiative. They developed recommendations for an Open Access publishing process, “which has worked for the past decade to provide the public with unrestricted, free access to scholarly research—much of which is publicly funded. Making the research publicly available to everyone—free of charge and without most copyright and licensing restrictions—will accelerate scientific research efforts and allow authors to reach a larger number of readers” (reference: http://www.budapestopenaccessinitiative.org)
\n\nIntechOpen’s co-founders, both scientists themselves, created the company while undertaking research in robotics at Vienna University. Their goal was to spread research freely “for scientists, by scientists’ to the rest of the world via the Open Access publishing model. The company soon became a signatory of the Budapest Initiative, which currently has more than 1000 supporting organizations worldwide, ranging from universities to funders.
\n\nAt IntechOpen today, we are still as committed to working with organizations and people who care about scientific discovery, to putting the academic needs of the scientific community first, and to providing an Open Access environment where scientists can maximize their contribution to scientific advancement. By opening up access to the world’s scientific research articles and book chapters, we aim to facilitate greater opportunity for collaboration, scientific discovery and progress. We subscribe wholeheartedly to the Open Access definition:
\n\n“By “open access” to [peer-reviewed research literature], we mean its free availability on the public internet, permitting any users to read, download, copy, distribute, print, search, or link to the full texts of these articles, crawl them for indexing, pass them as data to software, or use them for any other lawful purpose, without financial, legal, or technical barriers other than those inseparable from gaining access to the internet itself. The only constraint on reproduction and distribution, and the only role for copyright in this domain, should be to give authors control over the integrity of their work and the right to be properly acknowledged and cited” (reference: http://www.budapestopenaccessinitiative.org)
\n\nOAI-PMH
\n\nAs a firm believer in the wider dissemination of knowledge, IntechOpen supports the Open Access Initiative Protocol for Metadata Harvesting (OAI-PMH Version 2.0). Read more
\n\nLicense
\n\nBook chapters published in edited volumes are distributed under the Creative Commons Attribution 3.0 Unported License (CC BY 3.0). IntechOpen upholds a very flexible Copyright Policy. There is no copyright transfer to the publisher and Authors retain exclusive copyright to their work. All Monographs/Compacts are distributed under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Read more
\n\nPeer Review Policies
\n\nAll scientific works are Peer Reviewed prior to publishing. Read more
\n\nOA Publishing Fees
\n\nThe Open Access publishing model employed by IntechOpen eliminates subscription charges and pay-per-view fees, enabling readers to access research at no cost. In order to sustain operations and keep our publications freely accessible we levy an Open Access Publishing Fee for manuscripts, which helps us cover the costs of editorial work and the production of books. Read more
\n\nDigital Archiving Policy
\n\nIntechOpen is committed to ensuring the long-term preservation and the availability of all scholarly research we publish. We employ a variety of means to enable us to deliver on our commitments to the scientific community. Apart from preservation by the Croatian National Library (for publications prior to April 18, 2018) and the British Library (for publications after April 18, 2018), our entire catalogue is preserved in the CLOCKSS archive.
\n\nOpen Science is transparent and accessible knowledge that is shared and developed through collaborative networks.
\n\nOpen Science is about increased rigour, accountability, and reproducibility for research. It is based on the principles of inclusion, fairness, equity, and sharing, and ultimately seeks to change the way research is done, who is involved and how it is valued. It aims to make research more open to participation, review/refutation, improvement and (re)use for the world to benefit.
\n\nOpen Science refers to doing traditional science with more transparency involved at various stages, for example by openly sharing code and data. It implies a growing set of practices - within different disciplines - aiming at:
\n\nWe aim at improving the quality and availability of scholarly communication by promoting and practicing:
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His studies in robotics lead him not only to a PhD degree but also inspired him to co-found and build the International Journal of Advanced Robotic Systems - world's first Open Access journal in the field of robotics.",institutionString:null,institution:{name:"TU Wien",country:{name:"Austria"}}},{id:"441",title:"Ph.D.",name:"Jaekyu",middleName:null,surname:"Park",slug:"jaekyu-park",fullName:"Jaekyu Park",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/441/images/1881_n.jpg",biography:null,institutionString:null,institution:{name:"LG Corporation (South Korea)",country:{name:"Korea, South"}}},{id:"465",title:"Dr",name:"Christian",middleName:null,surname:"Martens",slug:"christian-martens",fullName:"Christian Martens",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:null},{id:"479",title:"Dr.",name:"Valentina",middleName:null,surname:"Colla",slug:"valentina-colla",fullName:"Valentina Colla",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/479/images/358_n.jpg",biography:null,institutionString:null,institution:{name:"Sant'Anna School of Advanced Studies",country:{name:"Italy"}}},{id:"494",title:"PhD",name:"Loris",middleName:null,surname:"Nanni",slug:"loris-nanni",fullName:"Loris Nanni",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/494/images/system/494.jpg",biography:"Loris Nanni received his Master Degree cum laude on June-2002 from the University of Bologna, and the April 26th 2006 he received his Ph.D. in Computer Engineering at DEIS, University of Bologna. 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After finishing his P. hD degree in 1992, he served in the Industry as a Scientific Officer and continued his academic career as a visiting scholar for a number of educational institutions. In 1996 he joined National University of Science & Technology Pakistan (NUST) as an Associate Professor; NUST is one of the top few universities in Pakistan. In 1999 he joined an International Company Lineo Inc, Canada as Manager Compiler Group, where he headed the group for developing Compiler Tool Chain and Porting of Operating Systems for the BLACKfin processor. The processor development was a joint venture by Intel and Analog Devices. In 2002 Lineo Inc., was taken over by another company, so he joined Aalborg University Denmark as an Assistant Professor.\nProfessor Akbar has truly a multi-disciplined career and he continued his legacy and making progress in many areas of his interests both in teaching and research. 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Recently, bioinspired systems have been successfully employing biomechanics to develop and improve assistive technology and rehabilitation devices. The research topic "Bioinspired Technology and Biomechanics" welcomes studies reporting recent advances in bioinspired technologies that contribute to individuals\' health, inclusion, and rehabilitation. Possible contributions can address (but are not limited to) the following research topics: Bioinspired design and control of exoskeletons, orthoses, and prostheses; Experimental evaluation of the effect of assistive devices (e.g., influence on gait, balance, and neuromuscular system); Bioinspired technologies for rehabilitation, including clinical studies reporting evaluations; Application of neuromuscular and biomechanical models to the development of bioinspired technology.',annualVolume:11404,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/8.jpg",editor:{id:"144937",title:"Prof.",name:"Adriano",middleName:"De Oliveira",surname:"Andrade",fullName:"Adriano Andrade",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRC8QQAW/Profile_Picture_1625219101815",institutionString:null,institution:{name:"Federal University of Uberlândia",institutionURL:null,country:{name:"Brazil"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"49517",title:"Prof.",name:"Hitoshi",middleName:null,surname:"Tsunashima",fullName:"Hitoshi Tsunashima",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYTP4QAO/Profile_Picture_1625819726528",institutionString:null,institution:{name:"Nihon University",institutionURL:null,country:{name:"Japan"}}},{id:"425354",title:"Dr.",name:"Marcus",middleName:"Fraga",surname:"Vieira",fullName:"Marcus Vieira",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003BJSgIQAX/Profile_Picture_1627904687309",institutionString:null,institution:{name:"Universidade Federal de Goiás",institutionURL:null,country:{name:"Brazil"}}},{id:"196746",title:"Dr.",name:"Ramana",middleName:null,surname:"Vinjamuri",fullName:"Ramana Vinjamuri",profilePictureURL:"https://mts.intechopen.com/storage/users/196746/images/system/196746.jpeg",institutionString:"University of Maryland, Baltimore County",institution:{name:"University of Maryland, Baltimore County",institutionURL:null,country:{name:"United States of America"}}}]},{id:"9",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering",keywords:"Biotechnology, Biosensors, Biomaterials, Tissue Engineering",scope:"The Biotechnology - Biosensors, Biomaterials and Tissue Engineering topic within the Biomedical Engineering Series aims to rapidly publish contributions on all aspects of biotechnology, biosensors, biomaterial and tissue engineering. We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics can include but are not limited to: Biotechnology such as biotechnological products and process engineering; Biotechnologically relevant enzymes and proteins; Bioenergy and biofuels; Applied genetics and molecular biotechnology; Genomics, transcriptomics, proteomics; Applied microbial and cell physiology; Environmental biotechnology; Methods and protocols. Moreover, topics in biosensor technology, like sensors that incorporate enzymes, antibodies, nucleic acids, whole cells, tissues and organelles, and other biological or biologically inspired components will be considered, and topics exploring transducers, including those based on electrochemical and optical piezoelectric, thermal, magnetic, and micromechanical elements. Chapters exploring biomaterial approaches such as polymer synthesis and characterization, drug and gene vector design, biocompatibility, immunology and toxicology, and self-assembly at the nanoscale, are welcome. Finally, the tissue engineering subcategory will support topics such as the fundamentals of stem cells and progenitor cells and their proliferation, differentiation, bioreactors for three-dimensional culture and studies of phenotypic changes, stem and progenitor cells, both short and long term, ex vivo and in vivo implantation both in preclinical models and also in clinical trials.",annualVolume:11405,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/9.jpg",editor:{id:"126286",title:"Dr.",name:"Luis",middleName:"Jesús",surname:"Villarreal-Gómez",fullName:"Luis Villarreal-Gómez",profilePictureURL:"https://mts.intechopen.com/storage/users/126286/images/system/126286.jpg",institutionString:null,institution:{name:"Autonomous University of Baja California",institutionURL:null,country:{name:"Mexico"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"35539",title:"Dr.",name:"Cecilia",middleName:null,surname:"Cristea",fullName:"Cecilia Cristea",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYQ65QAG/Profile_Picture_1621007741527",institutionString:null,institution:{name:"Iuliu Hațieganu University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"40735",title:"Dr.",name:"Gil",middleName:"Alberto Batista",surname:"Gonçalves",fullName:"Gil Gonçalves",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYRLGQA4/Profile_Picture_1628492612759",institutionString:null,institution:{name:"University of Aveiro",institutionURL:null,country:{name:"Portugal"}}},{id:"211725",title:"Associate Prof.",name:"Johann F.",middleName:null,surname:"Osma",fullName:"Johann F. 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