\\n\\n
IntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\\n\\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\\n\\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\\n\\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\\n\\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\\n\\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\\n\\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\\n\\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\\n\\nFeel free to share this news on social media and help us mark this memorable moment!
\\n\\n\\n"}]',published:!0,mainMedia:{caption:"",originalUrl:"/media/original/237"}},components:[{type:"htmlEditorComponent",content:'
After years of being acknowledged as the world's leading publisher of Open Access books, today, we are proud to announce we’ve successfully launched a portfolio of Open Science journals covering rapidly expanding areas of interdisciplinary research.
\n\n\n\nIntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\n\nBy listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
\n\nAll three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\n\n"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\n\n"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\n\nIn conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\n\n“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\n\nWe invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\n\nFeel free to share this news on social media and help us mark this memorable moment!
\n\n\n'}],latestNews:[{slug:"intechopen-supports-asapbio-s-new-initiative-publish-your-reviews-20220729",title:"IntechOpen Supports ASAPbio’s New Initiative Publish Your Reviews"},{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"}]},book:{item:{type:"book",id:"10459",leadTitle:null,fullTitle:"Light-Emitting Diodes and Photodetectors - Advances and Future Directions",title:"Light-Emitting Diodes and Photodetectors",subtitle:"Advances and Future Directions",reviewType:"peer-reviewed",abstract:"This book provides a detailed overview of the most recent advances in the fascinating world of light-emitting diodes (LEDs), organic light-emitting diodes (OLEDs), and photodetectors (PDs). Chapters in Section 1 discuss the different types and designs of LEDs/OLEDs and their use in light output, color rendering, and more. Chapters in Section 2 examine innovative structures, emerging materials, and physical effects of PDs. This book is a useful resource for students and scientists working in the field of photonics and advanced technologies.",isbn:"978-1-83968-556-9",printIsbn:"978-1-83968-555-2",pdfIsbn:"978-1-83968-564-4",doi:"10.5772/intechopen.92474",price:119,priceEur:129,priceUsd:155,slug:"light-emitting-diodes-and-photodetectors-advances-and-future-directions",numberOfPages:206,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"3e9039cfb58370a6596abf61dfaf4973",bookSignature:"Maurizio Casalino and Jagannathan Thirumalai",publishedDate:"September 29th 2021",coverURL:"https://cdn.intechopen.com/books/images_new/10459.jpg",numberOfDownloads:2530,numberOfWosCitations:1,numberOfCrossrefCitations:4,numberOfCrossrefCitationsByBook:1,numberOfDimensionsCitations:5,numberOfDimensionsCitationsByBook:1,hasAltmetrics:0,numberOfTotalCitations:10,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"August 25th 2020",dateEndSecondStepPublish:"September 22nd 2020",dateEndThirdStepPublish:"November 21st 2020",dateEndFourthStepPublish:"February 9th 2021",dateEndFifthStepPublish:"April 10th 2021",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"106767",title:"Dr.",name:"Maurizio",middleName:null,surname:"Casalino",slug:"maurizio-casalino",fullName:"Maurizio Casalino",profilePictureURL:"https://mts.intechopen.com/storage/users/106767/images/system/106767.jpg",biography:'Dr. Maurizio Casalino earned a Ph.D. in Electrical Engineering from the University “Mediterranea” of Reggio Calabria, Italy, after obtaining a laurea (summa cum laude) in Electrical Engineering from University of Naples \\"Federico II.” In 2010, he joined the Institute of Applied Sciences and Intelligent Systems, Naples, as a researcher. He taught Optoelectronics at the University of Calabria from 2009 to 2012, physics of the semiconductors and devices and analog electronics from 2013 to 2021, and from 2020 to 2021, respectively, at the University of Campania “Luigi Vanvitelli”. \n\nHe is the author of more than ninety scientific articles focused on the development of optoelectronic and photonic devices. His research activities have been funded by CNR (National Research Council of Italy), public and external organizations, and the European Community.',institutionString:"National Research Council",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"3",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"National Research Council",institutionURL:null,country:{name:"Italy"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:{id:"99242",title:"Prof.",name:"Jagannathan",middleName:null,surname:"Thirumalai",slug:"jagannathan-thirumalai",fullName:"Jagannathan Thirumalai",profilePictureURL:"https://mts.intechopen.com/storage/users/99242/images/system/99242.png",biography:"Dr. J. Thirumalai received his Ph.D. from Alagappa University, Karaikudi in 2010. He was also awarded the Post-doctoral Fellowship from Pohang University of Science and Technology (POSTECH), Republic of Korea, in 2013. He worked as Assistant Professor of Physics, B.S. Abdur Rahman University, Chennai, India (2011 to 2016). Currently, he is working as Senior Assistant Professor of Physics, Srinivasa Ramanujan Centre, SASTRA Deemed University, Kumbakonam (T.N.), India. His research interests focus on luminescence, self-assembled nanomaterials, and thin film opto-electronic devices. He has published more than 60 SCOPUS/ISI indexed papers and 11 book chapters, edited 4 books and member in several national and international societies like RSC, OSA, etc. Currently, he served as a principal investigator for a funded project towards the application of luminescence based thin film opto-electronic devices, funded by the Science and Engineering Research Board (SERB), India. As an expert in opto-electronics and nanotechnology area, he has been invited as external and internal examiners to MSc and PhD theses, invited to give talk in some forum, review papers for international and national journals.",institutionString:"SASTRA University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"10",totalChapterViews:"0",totalEditedBooks:"6",institution:null},coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"755",title:"Photonics",slug:"electrical-and-electronic-engineering-photonics"}],chapters:[{id:"77274",title:"Conducting Polymer-Based Emissive Layer on Efficiency of OLEDs",doi:"10.5772/intechopen.98652",slug:"conducting-polymer-based-emissive-layer-on-efficiency-of-oleds",totalDownloads:265,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Many changes have arisen in the world of display technologies as time has passed. In the vast area of display technology, Organic light-emitting diode is a recent and exciting discovery. Organic light-emitting diodes (OLEDs) have received a lot of curiosity among the researcher in recent years as the next generation of lighting and displays due to their numerous advantages, such as superior efficiency, mechanical flexibility and stability, chemical versatility, ease of fabrication, and so on. It works on the theory of electroluminescence, which is a mechanism in which electrical energy converts to light energy. Organic LEDs have a thickness of 100 to 500 nanometers or 200 times that of human hair. In OLEDs, organic material can be used in two or three layers. The emissive layer plays a key role in OLEDs. Polymers are used in the emissive layer to enhance the efficiency of OLEDs at the same time self-luminescence materials are used in OLEDs. In displays, this self-illuminating property removes the need for backlighting. Compared to LEDs and LCDs, OLED displays are smaller, lighter, and more portable.",signatures:"Debashish Nayak and Ram Bilash Choudhary",downloadPdfUrl:"/chapter/pdf-download/77274",previewPdfUrl:"/chapter/pdf-preview/77274",authors:[{id:"334427",title:"Ph.D.",name:"Debashish",surname:"Nayak",slug:"debashish-nayak",fullName:"Debashish Nayak"},{id:"415407",title:"Prof.",name:"Ram Bilash",surname:"Choudhary",slug:"ram-bilash-choudhary",fullName:"Ram Bilash Choudhary"}],corrections:null},{id:"74673",title:"Economic Applications for LED Lights in Industrial Sectors",doi:"10.5772/intechopen.95412",slug:"economic-applications-for-led-lights-in-industrial-sectors",totalDownloads:355,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:0,abstract:"After the Introduction, which discuss the main advantages and disadvantage of LED from Economics angle, the entire Chapter is presented in three sections. The first section discusses the economic benefits of replacing different types and rating of outdoor HID lights, typically installed in an industrial plant, with LED lighting. The section determines important economic indicators to evaluate direct and indirect benefits that can be achieved from using LED lights. In second section an efficient, safe and cost effective design to automate LED lighting system used for long roads with low-traffic is provided. The section provides smart control using image recognition for cost saving of road lighting operation and gives economic analysis for this lighting system. In third section, design of intelligent daylight utilization to achieve efficient indoor lighting intensity control for LED lights that are used in industrial building is provided. Comprehensive evaluation of the lighting system economics is discussed.",signatures:"Muhammad M.A.S. Mahmoud",downloadPdfUrl:"/chapter/pdf-download/74673",previewPdfUrl:"/chapter/pdf-preview/74673",authors:[{id:"150046",title:"Prof.",name:"Muhammad M.A.S.",surname:"Mahmoud",slug:"muhammad-m.a.s.-mahmoud",fullName:"Muhammad M.A.S. Mahmoud"}],corrections:null},{id:"76115",title:"Passive and Active Topologies Investigation for LED Driver Circuits",doi:"10.5772/intechopen.97098",slug:"passive-and-active-topologies-investigation-for-led-driver-circuits",totalDownloads:391,totalCrossrefCites:1,totalDimensionsCites:1,hasAltmetrics:0,abstract:"In this chapter, a survey of LED driver circuits is presented. The driver circuit is a crucial component in the LED light system. It provides the correct voltage and current values for the best brightness and long life. Furthermore, the driver circuits contribute to obtaining high efficiency and reliability light system. Several lighting applications need different driver topologies that meet the use requirement and the energy sources available. In actual applications, passive and active circuits are implemented to satisfy the LED driver electrical requirements and cost-effective demands. The LED driver circuits investigation evaluate the issues and the solutions in the LED lighting systems connected to a DC source such as a battery or AC line. The AC line connection requisites such as the power factor correction and the harmonic distortion are dealt with both the driver topology and control optimization. Also, the volume reduction need is examined in the circuitry choice. Moreover, the different topologies of the power converters isolated and not isolated used in the driver circuits based on both the power request and supply source are described and critically evaluated.",signatures:"Salvatore Musumeci",downloadPdfUrl:"/chapter/pdf-download/76115",previewPdfUrl:"/chapter/pdf-preview/76115",authors:[{id:"335428",title:"Ph.D.",name:"Salvatore",surname:"Musumeci",slug:"salvatore-musumeci",fullName:"Salvatore Musumeci"}],corrections:null},{id:"78120",title:"Near-Infrared Schottky Silicon Photodetectors Based on Two Dimensional Materials",doi:"10.5772/intechopen.99625",slug:"near-infrared-schottky-silicon-photodetectors-based-on-two-dimensional-materials",totalDownloads:220,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Since its discovery in 2004, graphene has attracted the interest of the scientific community due to its excellent properties of high carrier mobility, flexibility, strong light-matter interaction and broadband absorption. Despite of its weak light optical absorption and zero band gap, graphene has demonstrated impressive results as active material for optoelectronic devices. This success pushed towards the investigation of new two-dimensional (2D) materials to be employed in a next generation of optoelectronic devices with particular reference to the photodetectors. Indeed, most of 2D materials can be transferred on many substrates, including silicon, opening the path to the development of Schottky junctions to be used for the infrared detection. Although Schottky near-infrared silicon photodetectors based on metals are not a new concept in literature the employment of two-dimensional materials instead of metals is relatively new and it is leading to silicon-based photodetectors with unprecedented performance in the infrared regime. This chapter aims, first to elucidate the physical effect and the working principles of these devices, then to describe the main structures reported in literature, finally to discuss the most significant results obtained in recent years.",signatures:"Teresa Crisci, Luigi Moretti, Mariano Gioffrè and Maurizio Casalino",downloadPdfUrl:"/chapter/pdf-download/78120",previewPdfUrl:"/chapter/pdf-preview/78120",authors:[{id:"106767",title:"Dr.",name:"Maurizio",surname:"Casalino",slug:"maurizio-casalino",fullName:"Maurizio Casalino"},{id:"346015",title:"Dr.",name:"Teresa",surname:"Crisci",slug:"teresa-crisci",fullName:"Teresa Crisci"},{id:"346017",title:"Prof.",name:"Luigi",surname:"Moretti",slug:"luigi-moretti",fullName:"Luigi Moretti"},{id:"346018",title:"Dr.",name:"Mariano",surname:"Gioffrè",slug:"mariano-gioffre",fullName:"Mariano Gioffrè"}],corrections:null},{id:"74722",title:"Photo-Detectors Based on Two Dimensional Materials",doi:"10.5772/intechopen.95559",slug:"photo-detectors-based-on-two-dimensional-materials",totalDownloads:301,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"2D materials like transition metal dichalcogenides, black phosphorous, silicene, graphene are at the forefront of being the most potent 2D materials for optoelectronic applications because of their exceptional properties. Several application-specific photodetectors based on 2D materials have been designed and manufactured due to a wide range and layer-dependent bandgaps. Different 2D materials stacked together give rise to many surprising electronic and optoelectronic phenomena of the junctions based on 2D materials. This has resulted in a lot of popularity of 2D heterostructures as compared to the original 2D materials. This chapter presents the progress of optoelectronic devices (photodetectors) based on 2D materials and their heterostructures.",signatures:"Mubashir A. Kharadi, Gul Faroz A. Malik and Farooq A. Khanday",downloadPdfUrl:"/chapter/pdf-download/74722",previewPdfUrl:"/chapter/pdf-preview/74722",authors:[{id:"331214",title:"Dr.",name:"Mubashir A.",surname:"Ahmad",slug:"mubashir-a.-ahmad",fullName:"Mubashir A. Ahmad"},{id:"332399",title:"Mr.",name:"Gul-Faroz Ahmad",surname:"Malik",slug:"gul-faroz-ahmad-malik",fullName:"Gul-Faroz Ahmad Malik"},{id:"335891",title:"Dr.",name:"Farooq Ahmad",surname:"Khanday",slug:"farooq-ahmad-khanday",fullName:"Farooq Ahmad Khanday"}],corrections:null},{id:"75114",title:"Two-Dimensional Group-10 Noble-Transition-Metal Dichalcogenides Photodetector",doi:"10.5772/intechopen.95883",slug:"two-dimensional-group-10-noble-transition-metal-dichalcogenides-photodetector",totalDownloads:467,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"2D Transition-Metal Dichalcogenides (TMDs) have been widely considered as a promising material for future optoelectronics due to the strong light-matter interaction, fantastic electronic properties and environmental stability. However, the relatively large bandgap and low mobility of conventional TMDs (such as MoS2 and WS2) limit their applications in infra optoelectronics and high-speed photodetection. In this chapter, we introduce a new type of group-10 noble TMDs (NTMDs), which exhibit outstanding properties such as unique structural phase, widely tunable energy gap and high mobility. Till now, various NTMDs-based photodetectors have been realized with ultrabroad detection waveband (200 nm to 10.6 μm), fast response time, high responsivity and detectivity, and polarization sensitivity. NTMDs have been excellent potential candidates for next-generation photodetection devices with high-performance, wafer-scalability and flexibility.",signatures:"Haoran Mu, Jian Yuan and Shenghuang Lin",downloadPdfUrl:"/chapter/pdf-download/75114",previewPdfUrl:"/chapter/pdf-preview/75114",authors:[{id:"329900",title:"Prof.",name:"Shenghuang",surname:"Lin",slug:"shenghuang-lin",fullName:"Shenghuang Lin"},{id:"344332",title:"Dr.",name:"Haoran",surname:"Mu",slug:"haoran-mu",fullName:"Haoran Mu"},{id:"344333",title:"Dr.",name:"Jian",surname:"Yuan",slug:"jian-yuan",fullName:"Jian Yuan"}],corrections:null},{id:"74602",title:"Group III-Nitrides and Their Hybrid Structures for Next-Generation Photodetectors",doi:"10.5772/intechopen.95389",slug:"group-iii-nitrides-and-their-hybrid-structures-for-next-generation-photodetectors",totalDownloads:290,totalCrossrefCites:1,totalDimensionsCites:2,hasAltmetrics:0,abstract:"In the last few decades, there has been a phenomenal rise and evolution in the field of III–Nitride semiconductors for optoelectronic applications such as lasers, sensors and detectors. However, certain hurdles still remain in the path of designing high-performance photodetectors (PDs) based on III-Nitride semiconductors considering their device performance. Recently, a lot of progress has been achieved in devices based on the high quality epilayers grown by molecular beam epitaxy (MBE). Being an ultra-high vacuum environment based-technique, MBE has enabled the realization of high-quality and highly efficient PDs which have exhibited competitive figures of merit to that of the commercial PDs. Moreover, by combining the novel properties of 2D materials with MBE-grown III-Nitrides, devices with enhanced functionalities have been realized which would pave a way towards the next-generation photonics. In the current chapter, the basic concepts about photodetection have been presented in detail, followed by a discussion on the basic properties of the III-Nitride semiconductors, and the recent advancements in the field of MBE-grown III-Nitrides-based PDs, with an emphasis on their hybrid structures. Finally, an outlook has been provided highlighting the present shortcomings as well as the unresolved issues associated with the present-day devices in this emerging field of research.",signatures:"Deependra Kumar Singh, Basanta Kumar Roul, Karuna Kar Nanda and Saluru Baba Krupanidhi",downloadPdfUrl:"/chapter/pdf-download/74602",previewPdfUrl:"/chapter/pdf-preview/74602",authors:[{id:"196960",title:"Dr.",name:"Basanta",surname:"Roul",slug:"basanta-roul",fullName:"Basanta Roul"},{id:"328314",title:"Emeritus Prof.",name:"Saluru Baba",surname:"Krupanidhi",slug:"saluru-baba-krupanidhi",fullName:"Saluru Baba Krupanidhi"},{id:"331999",title:"Mr.",name:"Deependra Kumar",surname:"Singh",slug:"deependra-kumar-singh",fullName:"Deependra Kumar Singh"},{id:"332001",title:"Prof.",name:"Karuna Kar",surname:"Nanda",slug:"karuna-kar-nanda",fullName:"Karuna Kar Nanda"}],corrections:null},{id:"74765",title:"Studying a LW-VCSEL-Based Resonant Cavity Enhanced Photodetector and Its Application in Microwave Photonics Circuits",doi:"10.5772/intechopen.95560",slug:"studying-a-lw-vcsel-based-resonant-cavity-enhanced-photodetector-and-its-application-in-microwave-ph",totalDownloads:246,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"A detailed comparative experimental study was carried out to pursue advanced performances corresponding to the key parameters of two photodetectors based on vertical cavity surface emitting laser (VCSEL) operating in free-running or optically injection locked mode, as well as an inherent pin-photodetector. During the preliminary study, the key static and dynamic parameters were quantitatively determined and the optimal operating modes were derived for the both versions of VCSEL-based photodetectors as separate microwave-photonics circuit elements. Based on them, a final experiment was conducted to evaluate the processing quality, when one of the versions of VCSEL-based photodetectors or a inherent pin-photodetector is implemented as an optical-to-electrical converter for a typical microwave-photonics circuit that processes 120-Mbps 16-position quadrature amplitude modulated signal on the radio frequency carrier of 1–6 GHz. As a result, it was confirmed that better processing quality, i.e. Error Vector Magnitude value of less than 4%, could be obtained by using the free-running VCSEL-based photodetector version.",signatures:"Mikhail E. Belkin, Leonid I. Zhukov, Dmitriy A. Fofanov, Mikhail G. Vasil’ev and Alexander S. 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The number of people with type 2 diabetes mellitus (T2DM) is growing rapidly worldwide and has already exceeded 530 million in 2021 [1]. Because of the severe consequences of T2DM for patients and the enormous burden on the healthcare system, a lot of research is focused on understanding the development of T2DM. The healthy pancreatic beta cells secrete insulin at a basal rate throughout the day and increase secretion in response to stimulation with nutrients, especially glucose, and other neurohormonal secretagogues, such as acetylcholine and GLP-1, after a meal [2, 3, 4]. Understanding the pathophysiology of early phases of glucose tolerance disruption associated with morphological and functional beta cell changes present in insulin-resistant people susceptible to the development of T2DM is especially important and has been the focus of many research groups [5, 6, 7, 8, 9]. However, to date very limited information is available about the ultrastructural alterations of beta cells during the early stages of T2DM development [10, 11, 12, 13].
Thus, the aim of our chapter is to present the importance and illustrate the usefulness of transmission electron microscopy (TEM) in the research field of pancreas physiology through specific and easily reproducible examples. TEM is a technique used to obtain ultrahigh-resolution images of different samples. The prototype of the transmission electron microscope was developed by Ernst Ruska and Max Knoll in 1931. Since then, TEM has been extensively used in biomedical research, helping us deepen our knowledge about the ultrastructure of cells and understanding the cellular processes. TEM was first used to identify specific features of pancreatic endocrine and exocrine cells in the mid-1950s by Paul Lacy [14] and George Palade [15], respectively. TEM exploits the wavelength properties of electrons to provide greater spatial resolution than the resolution achieved using photons in light microscopy (LM). In TEM, a beam of high-voltage electrons is emitted by the electron gun and then passed through the sample. The most challenging part of TEM is the preparation of samples that are thin and robust enough to allow electrons to penetrate the sample on the one hand and survive the damage caused by the electron beam on the other. In this chapter, TEM is implemented as an appropriate research method that allows us to detect very subtle structural changes in pancreas cells of mice fed with a western diet (WD). Here, we focus on sample preparation, microscopy, and quantitative analysis of ultrastructure, together with some representative results, to illustrate the utility and emphasize the importance of TEM in pancreas research. A comprehensive analysis of ultrastructural changes in WD-fed mice with partly compensated diabetes mellitus will be present in detail elsewhere.
In the first part of this chapter, we briefly summarize the basic anatomical features of the pancreas in humans and mice and describe the main physiological characteristics of the endocrine and exocrine pancreas. Next, we describe the development of T2DM, focusing on the role of obesity and key pathophysiological events. In the central part of this chapter, we discuss the ultrastructural morphology and describe the methodology used for ultrastructural morphometry of the exocrine and endocrine pancreas during the early stages of WD-induced T2DM development.
The pancreas is an unpaired gland of the alimentary tract with two different but complementary functions: the production of digestive enzymes and alkaline fluid in exocrine cells that help with the breakdown of energy-rich nutrients and the synthesis by endocrine cells of hormones needed to control the storage and usage of energy-rich nutrients [16]. In humans, the pancreas is a well-defined organ with three major parts: the head, the body, and the tail, extending from the duodenum to the spleen [17, 18]. In mice, the pancreas is not as well-defined as in humans but is rather diffusely distributed in a dendritic manner while it is still composed of three major parts: the duodenal lobe, the largest splenic lobe, and the smallest gastric lobe [19]. A fibrous capsule surrounds the pancreas, and the connective tissue extending into the gland divides the parenchyma into larger lobes and smaller lobules [18, 20]. Each lobule is composed of acini that consists of pyramid-shaped acinar cells [21]. The exocrine part accounts for 96–99% and the endocrine islets of Langerhans for the remaining 1–4% of total pancreas parenchyma [22, 23]. In mice, the islets are mainly interlobular, while in humans they are usually intralobular, mostly on the edge of lobules [24, 25]. Despite some differences [26], islets of Langerhans from mice and men possess many structural and functional similarities [27, 28]. They are round to oval, vary in size, and range from a few to several thousand endocrine cells. Importantly, the islet size distributions are similar in humans and mice (and also many other species) [22]. At least five different types of endocrine cells can be found in islets in both species [27]. Most numerous are the beta cells that synthesize and secrete insulin. Alpha cells secrete glucagon, while delta cells and PP cells release somatostatin and pancreatic polypeptide, respectively. Finally, epsilon cells are the least abundant, synthesizing and secreting ghrelin [23, 24, 25].
As mentioned above, the exocrine pancreas plays a crucial role in the enzymatic digestion of carbohydrates, proteins, and lipids and secretes a bicarbonate-rich fluid [29, 30, 31]. The enzymes are stored in an inactive proenzyme form in the so-called zymogene granules located at the apical membrane of acinar cells and are released in response to stimulation by neurohormonal secretagogues, such as acetylcholine and cholecystokinin, via exocytosis. The fusion of granules with the apical plasma membrane releases the enzymes first into the acinar lumen, from where they pass via the ductal tree into the small intestine [32]. In contrast, islets release their hormones into the numerous islet blood capillaries that drain into the portal venous system. In the liver and other key target tissues, such as the skeletal muscle and adipose tissue, insulin acts as a key anabolic hormone, promoting glycogenesis, glycolysis, lipogenesis, and proteinogenesis, and suppressing gluconeogenesis, lipolysis, and proteolysis [33, 34, 35]. On the other hand, glucagon acts mainly in the liver, where it promotes glycogenolysis and gluconeogenesis, and inhibits glycolysis and glycogenesis [36, 37].
The decrease in insulin action indicates insulin resistance, the main hallmark of T2DM [38]. Several factors play a role in the development of insulin resistance, with obesity probably being the most important one [39]. During the last three decades, obesity has reached an epidemic stage in all age groups [40]. Increased intake of energy-dense foods containing a high percentage of fat and carbohydrates, combined with a lack of physical activity, leads to a net positive energy balance and represents the primary cause of obesity. The first stages of obesity are hypertrophy and hyperplasia, where adipocytes try to meet the demand to store excessive energy. When levels of free fatty acids and triglycerides exceed the metabolic capacity of adipose tissue, they accumulate as ectopic fat in non-adipose tissue such as the liver, pancreas, skeletal muscles, and heart [41]. The regional distribution of adipose tissue is particularly important for the development of disorders of glucose and lipid metabolism [42, 43]. The fat infiltration in the liver and pancreas in the absence of excess alcohol intake is termed non-alcoholic fatty liver disease (NAFLD) and non-alcoholic fatty pancreas disease (NAFPD), respectively [44]. Due to fat accumulation in hepatocytes, NAFLD leads to inflammation, fibrosis, cirrhosis, and liver cancer and critically affects insulin sensitivity in the liver [44, 45, 46]. NAFPD plays a similar role in the dysfunction of the endocrine and exocrine pancreas leading to exacerbation of acute pancreatitis and increasing the susceptibility to pancreatic cancer [46, 47, 48, 49, 50]. Although the concept of the NAFPD was introduced only a few years ago [51], the correlation between fat infiltration and pancreas was first described almost a century ago on obese cadavers having larger pancreata compared to non-obese cadavers [52, 53]. Importantly, over the last decade, it has been demonstrated convincingly that most of the morphological, functional, and clinical features of T2DM are reversible with sufficient weight loss and that these positive changes depend on the level of hepatic and pancreatic fat reduction [54, 55, 56].
Although insulin resistance is one of the main features of T2DM, it is not sufficient for the development of T2DM. Despite increasing insulin resistance, present long before the onset of diabetes, individuals with preserved beta cell capacity can stay normoglycemic for several years due to compensatory insulin hypersecretion. Furthermore, a certain proportion of individuals with insulin resistance never develop T2DM. The idea has been put forward that there may be a personal fat threshold or an individual level of susceptibility to developing T2DM at a given body mass index, with the main mechanisms behind this attractive hypothesis being varying degrees of fat accumulation in the liver and the pancreas, together with varying individual responses to this accumulation [57]. An obese subject with insulin resistance can secrete 2–5 times more insulin compared to lean non-diabetic individuals in response to a glycemic load, but when the adaptive capacity of the beta cells fails, T2DM occurs [58]. Several studies have shown that the first phase of insulin secretion is primarily affected, resulting in impaired glucose tolerance. During the progression of T2DM, the second phase of insulin secretion is further lost. Post-translational defects in insulin synthesis also occur, resulting in increased proinsulin secretion, and by the time the diagnosis of T2DM is made, the beta cell function is already typically reduced by 80% [59]. This beta cell failure is responsible for transitioning from an insulin-resistant compensated state to overt T2DM and remains to be elucidated in details. It probably involves an initially inadequate beta cell mass and an insufficiently increased response of the existing beta cells to increased insulin demand [60]. Noteworthy, the susceptibility of beta cells themselves to developing insulin resistance may play an important pathophysiological role [61]. Individuals with T2DM can display changes in beta cell mass, either due to a decrease in beta cell proliferation or/and an increase in cell apoptosis [62]. In T2DM patients, the apoptosis rate is increased severalfold compared to normoglycemic individuals [63], a phenomenon confirmed by ultrastructural analysis using TEM [64]. Furthermore, during T2DM gradual dedifferentiation of beta cells occurs in animal models, while the role of dedifferentiation during the development of human T2DM is controversial and less well-studied [63, 65, 66, 67, 68]. Chronic exposure of islets to elevated levels of glucose, fatty acids, and amino acids results in ER stress due to increased insulin synthesis and secretion [69]. The protein folding capacity of ER becomes exceeded, which leads to the activation of the so-called unfolded protein response (UPR) and consequently to the inhibition of protein translation [70, 71]. Gluco- and lipotoxicity cause a vicious cycle of continuous deterioration of the glucometabolic state and eventually impair insulin secretion and increase apoptosis. In T2DM, not only the islet cells are affected, but also the pancreatic acinar cell viability and growth decrease, leading to increased apoptosis and replacement by fat [72, 73]. Importantly, intracellular fat in acinar cells may affect beta cell function in a paracrine manner through the release of adipokines. It is also likely to play a role in pancreatic carcinogenesis, seems to be associated with changes in innervation, and may initiate an acinar-to-adipocyte trans-differentiation [56].
Glucose-induced insulin secretion is based on oxidative metabolism, thus mitochondrial function is of crucial importance for beta cells [74]. Mutations in mitochondrial DNA result in reduced insulin secretion and hyperglycemia [75]. The genetic background is not the only factor affecting mitochondrial function. The diabetic environment, with chronic hyperglycemia, can also affect mitochondria [76]. Such a milieu could be responsible for the progression of T2DM and the reduced capacity of insulin release in these patients. Mitochondria appear round-shaped and hypertrophic in endocrine cells from T2DM islets [76, 77, 78]. Besides mitochondria, the structure of other cellular organelles is also altered [79]. The volume density of the endoplasmic reticulum is typically increased, and autophagic vacuoles are present [78, 80, 81]. TEM is also a very useful technique that enables us to identify apoptotic cells [82].
Several studies showed that TEM is suitable to detect morphological characteristics of the diabetic pancreas, but only a few studies have reported results in quantitative terms. Due to the large variability and plasticity of cells and their organelles, especially mitochondria, qualitative evaluations are not sufficient [83]. Furthermore, quantitative morphometry is needed to evaluate subtle structural changes early during the progression of the metabolic syndrome that may precede overt functional changes occurring during the development of T2DM.
Since mouse models of T2DM exhibit comparable developmental features and can provide significant insight into the mechanisms of T2DM development in humans [84], they are widely used in these studies. From several animal models, C57Bl/6 J mice have been the most susceptible to the development of the metabolic syndrome and diet-induced diabetes [85, 86, 87, 88, 89]. We performed the morphological and morphometric evaluation of pancreatic endocrine and exocrine tissue obtained from C57Bl/6J mice fed with control diet (CD) or western diet (WD). During 8 weeks on WD, these mice developed obesity, hyperglycemia, and hyperinsulinemia (data not shown). Pancreatic tissue samples were taken in parallel with the preparation of acute pancreas tissue slices intended for electrophysiological studies, confocal calcium imaging, and expression analyses. Although the functional response to glucose in terms of intracellular calcium concentration changes and sensitivity of the exocytotic machinery to calcium were well preserved in WD mice (data not shown), TEM revealed severe morphological changes in the endocrine and exocrine part of the pancreas. In the following section, we demonstrate how to use image segmentation methods and ultrastructural morphometry to analyze and quantify structural changes in the rough endoplasmic reticulum, mitochondria, and exocytotic vesicles and vacuolization in the endocrine and endocrine part of the pancreas from control animals and animals fed with WD. These ultrastructural lesions presented below can be detected early during the progression of the metabolic syndrome and precede cell apoptosis, necrosis, fat infiltration, and overt functional changes. Therefore, TEM enables a more direct assessment of the functional characteristics of the pancreatic tissue and is therefore a suitable and probably the crucial method for detecting alterations associated with early pancreas dysfunction.
The study protocol was approved by the Administration for Food Safety, Veterinary Sector and Plant Protection, Ministry of agriculture, Forestry and Food Republic of Slovenia (approval number: U34401–12/2015/3). The study was conducted in strict accordance with all national and European recommendations pertaining to care and work with laboratory animals, and every effort was made to minimize animal suffering.
Experiments were performed on 20–22-week-old male C57BL/6 J (RRID: IMSR_JAX:000664) mice purchased from Charles River (Figure 1A). Upon weaning, mice were fed with a standard rodent diet (CD, R70, Lantmännen, Stockholm, Sweden) with 72% of kcal from carbohydrates, 10% from fat, and 18% from protein until 12 weeks of age. From 12 to 20 weeks of age, a control group continued to be fed with CD while the second group was fed with WD (D12079B, Research diets inc., New Jersey, USA) containing 43% kcal from carbohydrates, 40% from fats and 17% from proteins (Figure 1B). Water was available
Study design (A) timeline of the study. The part of the project shown in yellow is presented in this chapter. (B) Energy profile of control diet (CD) and western diet (WD).
Acute pancreas tissue slices from CD and WD mice were prepared as described previously [90, 91, 92]. For light microscopy (LM) and transmission electron microscopy (TEM), a small piece of the pancreatic splenic lobe was clamped using hemostatic forceps to avoid the leakage of agarose into this part of the pancreas. After removal from the body, small fragments of the pancreas were fixed in 2.45% glutaraldehyde and 2.45% paraformaldehyde in a 0.1M sodium cacodylate buffer (pH 7.4) at room temperature for 3 h, and at 4°C for 12 h. The tissue was washed in a 0.1 M sodium cacodylate buffer (pH 7.4) at room temperature for 4 h and post-fixed with 2% OsO4 at room temperature for 2 h. After washing in a 0.1 M sodium cacodylate buffer (pH 7.4) at room temperature for 3 hours, the tissue was dehydrated in a graded series of ethanol (50%, 70%, 90%, 96%, and 100%, each for 30 minutes at room temperature). The pieces of the tissue were embedded in TAAB embedding resin (Agar Scientific Ltd., Essex, England). For LM, semithin sections (2 μm) were stained with 0.5% toluidine blue in an aqueous solution. For TEM, ultrathin sections (70–75 nm) of the tissue were stained with uranyl acetate and lead citrate and analyzed with a Zeiss EM 902 transmission electron microscope.
The pancreas is composed of exocrine and endocrine tissue and this functional specialization can readily be recognized microscopically (Figure 2).
Mice are fed with control diet (CD). (A) and (B) Semithin sections of the pancreas. (A) Exocrine part of the pancreas is composed of acinar cells (one cell marked by the yellow dotted line), organized in acini (one acinus marked by the green dotted line) and lobules (one lobule marked by the red dotted line). (B) Endocrine cells forming a Langerhans islet are seen as brighter stained cells (red dotted line). (C–F) Ultrathin sections of the pancreas. (C) Exocrine cells are easily recognized by their large size, typical shape, by abundant rough endoplasmic reticulum, and secretory granules. (D) The majority of secretory granules are found in the apical part of the cell. (E) and (F) endocrine cells can be distinguished by the fine structure and size of their secretory granules. In all endocrine cells, well-developed rough endoplasmic reticulum, Golgi apparatus, and numerous mitochondria are seen. AC, acinar cell; α, alpha cell; β, beta cell; δ, delta cell; D, duct; IL, islet of Langerhans; GA, Golgi apparatus; M, mitochondrion; N, nucleus; nu, nucleolus; RER, rough endoplasmic reticulum; SG, secretory granules. Scale bars: (A) and (B) 50 μm; (C) 2.5 μm; (D) 2 μm; (E) 5 μm; (F) 2.5 μm.
The majority of tissue consists of pyramid-shaped exocrine cells (Figure 2A–C). These cells form clusters or acini around small ducts and are organized in lobes with thin fibrous tissue. The exocrine cells produce inactive digestive enzymes, seen in the cytoplasm packed in secretory vesicles (Figure 2C and D), and secrete them into the intercalated ducts which they surround (Figure 2D). In each acinus, the exocrine cells are located around the intercalated ducts, with their narrow apical parts oriented to the duct (Figure 2C and D). The exocrine cells have a round or oval nucleus (Figure 2C), located basally. The most prominent structure of the exocrine cells is the rough endoplasmic reticulum (Figure 2C) which is present in all different parts of the cell. Numerous round electron-dense secretory vesicles are seen in the perinuclear and apical cytoplasm (Figure 2C and D). The oval mitochondria (Figure 2C and D) are found in different parts of the cell.
The endocrine cells are distributed throughout the pancreas (Figure 2B) as interlobular positioned clusters of cells termed islets of Langerhans. In the islets of Langerhans (Figure 2B) alpha, beta and delta cells can be distinguished (Figure 2E and F). They are characterized by numerous secretory vesicles. Glucagon granules of alpha cells are large dense-core vesicles and some of them have a pale halo. The core of the granule is of a similar diameter to insulin granules, but the whole granule is smaller by at least 50% (200 nm vs. 350 nm). Insulin-containing vesicles in beta cells are the largest with large clear peripheral halos. Somatostatin-containing granules of delta cells in mice are the smallest and lozenge-shaped (Figure 2E and F) [93, 94, 95, 96]. In the cytoplasm of all types of endocrine cells, abundant rough endoplasmic reticulum, Golgi apparatus, and many oval mitochondria are present (Figure 2E and F) [97].
Comparing the ultrastructure of pancreatic cells in control mice (CD; Figure 2) and mice fed the western diet (WD; Figures 3A and B, 4B, 5E, 7E, and 8D), we observed many important differences. In the exocrine pancreas of WD mice many necrotic cells (Figures 3A and 4B) are present. In some acinar cells, lipid droplets are seen in the cytoplasm (Figure 3A). In many cells, numerous autophagic structures, i.e., autophagosomes, autolysosomes (Figure 3B), and residual bodies are found. The mitochondria (Figure 4B) and rough endoplasmic reticulum (Figure 5E) seem to be disorganized, therefore these structures were analyzed in detail.
Mice are fed with western diet (WD). (A) and (B) Ultrathin sections of the pancreas. (A) A necrotic acinar cell containing a lipid droplet in the cytoplasm. (B) Autophagosomes and autolysosomes in the cytoplasm of an acinar cell. AL, autolysosome; AP, autophagosome; L, lipid droplet; M, mitochondrion; N, nucleus; SG, secretory granules; RER, rough endoplasmic reticulum. Scale bars: (A) 2 μm; (B) 500 nm.
Shape descriptors. (A) Circularity versus roundness. (B) Sketch of a mitochondrion (gray) with an overlay of the best fit ellipse (yellow), the major axis of the best fit ellipse (blue), and the minor axis of the best fit ellipse (red). The major axis is used in determining roundness (
Analysis of the mitochondria from a CD and a WD mouse. Labeled mitochondria in the image of the exocrine pancreas from the (A) CD and (B) WD mouse. Analysis of the (C) surface area, (D) circularity, (E) roundness, (F) aspect ratio and (G) solidity of an image from CD and WD mouse. Data were pooled from the following number of ROIs from the CD/WD image: 14/16. Data were analyzed using the Mann–Whitney U test, p values are indicated on graphs.
The structure of the islets of Langerhans in mice fed by WD is non-compact, inhomogeneous, containing more extracellular spaces than in mice fed by CD. There are many necrotic cells in different parts of the islets. In the cytoplasm of endocrine cells, some lipid droplets and many autophagic structures, i.e., autophagosomes, autolysosomes, and residual bodies can be found. Structural differences are also seen in the mitochondria and rough endoplasmic reticulum.
Structural characteristics of mitochondria, rough endoplasmic reticulum, zymogen granules, and vacuoles were studied. To accurately analyze various cell compounds, it is necessary to select TEM images taken at the same magnification.
Since mitochondria are crucial for normal beta cell stimulus-secretion coupling and their ultrastructure is altered during the development of T2DM, we analyzed them in more detail. First, we outlined all the mitochondria in the visual field and calculated the surface area in nm2. To quantitatively assess the condition of mitochondria, we measured the following shape descriptors: circularity, roundness, aspect ratio of the best fit ellipse, and solidity using Fiji software (NIH) [98].
Circularity (C) is a shape parameter that can mathematically indicate the degree of similarity to a perfect circle. A value of 1.0 indicates a perfect circle. When the circularity value approaches 0.0, the shape becomes less and less circular. Circularity is defined by the equation
Roundness (R) on the other hand, characterized by
is similar to circularity, but it is insensitive to irregular borders along the perimeter of the mitochondria and takes into account the major axis of the best fit ellipse. For an illustrative explanation of the differences between circularity and roundness, see Figure 6A.
Quantitative analysis of the RER. Representative TEM images from a CD (A) and a WD (E) mouse. The quantification pipeline involves segmentation (B and F), a binary mask depicting RER cisternae in black and cytosol in white), followed by RER determination using particle analysis on the segmented image (C and G). Overlay of the binary masks on the TEM images (D and H), RER cisternae in red, and cytosol in green). Exemplary percentages of the area covered by the RER cisternae are shown in the top right (CD mouse) and bottom right (WD mouse.
From the best fit ellipse fit to each mitochondrion, the major and minor axes were determined, and the aspect ratio (AR) was calculated by the following equation:
AR measures the ratio of an object’s height to its width (Figure 6B). Therefore, the aspect ratio is equal to one for a perfect circle and increases with an increase in deformation.
At the end, solidity (S) was measured using the same Fiji software. Solidity describes the extent to which shape is convex or concave. Taking the area within the mitochondrion and dividing it by the area enclosed by a convex hull provides information about the solidity of the shape (Figure 6C). Solidity of a perfectly convex structure is 1, but when the structure becomes more concave, the solidity will deviate from 1. The solidity is defined by
In the bullet points that follow, we describe each analysis step in detail.
Open an image in Fiji.
Select the straight line tool from the navigation pane and draw a straight line across the scale bar. The straight line must be precisely the same length as the scale bar.
In the “Analyze” menu, select “Set Scale”. When the set scale dialog box opens, enter the value of a scale bar into the “Knowing Distance” box, and in the “Unit of length” box, determine the unit of the scale bar from the image. Click the “OK” button. Now you have set the scale for this particular image.
Use the “freehand selection” tool from the toolbar and encircle the first mitochondrion on the image. After the right mouse click within the selected encircled mitochondrion, choose “Add to ROI Manager” from the dialog box.
Now encircle the next mitochondrion and click “Add” in the ROI Manager. In the same way, encircle all the mitochondria in the image.
In the “Analyze” menu, select “Set measurements” and thick the desired parameters from the dialog box. For the analysis of mitochondria, we selected “Area” and “Shape descriptors”. Click the “OK” button.
Now everything is set to analyze all the mitochondria from the image. Select “Measure” in the “Analyze” menu, and a new window with results will pop out. Save the results by selecting “Save as” from the “File” menu of the “Results” window.
Now you can analyze the new image in the same way. Remember that the scale must be set again when analyzing the next image.
In Figure 4 one can observe the results of the above analysis on mitochondria of two representative images of the exocrine pancreas from a CD (Figure 4A) and a WD (Figure 4B) mouse.
Quantitative analysis of the surface area covered by the RER cisternae on TEM images can provide valuable insight into functional changes in both acinar and endocrine cells. Quantification of the TEM data, in general, involves manual annotation of structures of interest. This approach proved to be an extremely time-consuming process for quantification of the RER data, as the organelle forms a complex and interconnected network of cisternae. To overcome this issue, Trainable Weka Segmentation (TWS, available as Fiji/ImageJ plugin) provides a machine learning tool capable of automated segmentation [99]. A limited number of manual annotations on a sample (training) TEM image produces a classifier that can be applied to the remaining data to segment images automatically. The following pipeline describes steps for RER analysis:
Image segmentation: TWS produces a segmented image that reliably separates RER cisternae from the cytosol (Figure 5B and F, compare with Figure 5A and E, respectively).
Particle analysis: subsequent particle analysis on the segmented image excludes unwanted objects based on the size exclusion criterion that removes small objects that were detected mostly within RER cisternae (Figure 5C and G). If needed, manually delete unwanted objects.
Quantification of data: the resulting binary mask improves partitioning compared to TWS segmentation alone (Figure 5D and H). The resulting image segments allow for quantification of RER abundance, expressed as the relative area covered by RER cisternae (% RER area). This approach detected a large increase in % RER area in WD mice compared with CD mice in the given cell (42% vs. 18%) (Figure 5).
Zymogen granules form a reserve pool of digestive enzymes that are secreted from acinar cells after stimulation. The granules are well visible on TEM images and detecting a change in a number of granules per cell, cumulative granule area per cell, or granule size could suggest a functional change in their physiology. Manual counting of granules is the simplest method to quantify zymogen content; however, it relies on an assumption of typical granule size, and it is a relatively time-consuming approach. Alternatively, manual annotation of individual granules would provide data also on the granule size, but it is an even more time-consuming method that would effectively hamper analysis or limit it to a few cells only. To at least partially automate the quantification, we took advantage of the fact that zymogen granules have typical properties: (i) granules are spherical structures, and (ii) they appear electron-dense (i.e. dark) on TEM images (Figure 7A and E).
Quantitative analysis of the surface area of zymogen granules. Representative TEM images from CD (A) and WD (E) mice. Image thresholding and watershed separation produce a binary mask (B and E), zymogen granules in black, inset depicts exemplary partitioning of two touching granules). Individual granules are determined using particle analysis on the segmented images (C and G). Panels D and H depict an overlay of the granules on the grayscale TEM image (vesicles in red). Quantification of the granule cross-section area shown in the panel I for a given cell from the CD and the WD mouse. Data were pooled from the following number of ROIs from the CD/WD image: 35/27. Data were analyzed using the Mann–Whitney U test, p values are indicated on graphs.
The following brief instructions describe a useful procedure to partition the granules on a TEM image:
Image thresholding and separation: Since granules provide good contrast, thresholding the TEM image produces a rough binary mask of granules. Inherently, the thresholding procedure detects two or more granules in close proximity as uniform structures. We can reliably separate the touching granules using the watershed separation method [100]. This method interprets the input image as a topographical surface that is flooded with water, placing water sources in the local topographical minima, and placing dams where water from different sources meets. These dams constitute the watershed (i.e., a border between touching granules, Figure 7B inset). The method works best for smooth convex objects that do not overlap too much, and it efficiently separates granules after the initial thresholding (Figure 7B and E).
Particle analysis: Employ particle analysis to remove unwanted small objects in the cytosol that were detected with the thresholding. Limiting the minimal object size effectively removes the background noise (Figure 7C and G).
Quantification of the zymogen granules: The resulting binary mask detects individual granules (see the overlay in the Figure 7D and H). The segmentation data generally allow for a straightforward counting of granules, assessing the cumulative granule area (case the entire cell is imaged, example data not shown), and granule size. The latter decreased by 22% in the analyzed acinar cell from a WD treated mouse (Figure 7I).
Cytoplasmic vacuolization is an ultrastructural change associated with pathological alterations in pancreatic cells [101]. The vacuoles appear electron-lucent (i.e., bright) and inhomogeneous on TEM images, very similar to other structures, and this does not allow the use of image thresholding as a technique to effectively detect vacuoles. Since the number of vacuoles per cell is limited, manual annotation of the structures is feasible.
Manual detection of the vacuoles: Use Fiji/ImageJ in combination with the built-in ROI manager to outline the vacuoles, which results in a binary mask of the visible structures on the image (Figure 8B demonstrating an empty mask, since no vacuoles were visible on the image, and 8E shows several vacuoles that were seen within a cell). Overlaying the structures can serve as visual feedback for quality assessment (Figure 8C and F).
Quantitative analysis of the vacuoles. Representative TEM images from CD (A) and WD (E) mice. Manual annotation of vacuoles resulted in a binary mask (B and E), vacuoles in black; please note that no vacuoles were present in A. Panels C and D depict an overlay of the vacuole binary mask on the grayscale TEM image (vacuoles in red, cytoplasm in green). Percentages of vacuole cross-section areas are shown on the right for the CD (blue) and the WD (red) mouse.
Quantification of the vacuoles: Use data from the above step to measure the relative area covered by the vacuoles by dividing the cumulative vacuole area by the visible cytoplasm surface. Using this approach, we demonstrated that vacuoles are abundant in the acinar cell of WD treated mice (27% vs. 0% in WD vs. CD mice, Figure 8 right).
There are only a handful of studies on pancreatic exocrine and endocrine ultrastructure in mice under diabetogenic conditions. One of the reasons for this relative underrepresentation of such a translationally relevant topic in the literature may be the rather complex study design typically accompanying work with genetic or dietary mouse models and electron microscopy. An even more important reason may often be a lack of a toolbox to easily, objectively, and reproducibly analyze ultrastructural changes in exocrine and endocrine cells in a quantitative manner. In this chapter, we tried to specifically address this problem by providing the readers with a robust step-by-step approach and detailed instructions on how to quantify changes in the ultrastructure of mitochondria, rough endoplasmic reticulum, and secretory vesicles, as well as the presence of vacuoles, by means of shape descriptors, thresholding, manual selection, and machine-learning supported image segmentation, followed by different quantification steps employed in the open-source Fiji software. With some field-specific modifications, our analyses shall also be useful for many other life scientists.
We would like to express our gratitude to colleagues at the Institute of Physiology for their support and to Prof. Gerd Leitinger (Medical University Graz) for critical reading of the manuscript.
This research was funded by the SLOVENIAN RESEARCH AGENCY, grant number P3-0396, I0-0029, N3-0133, and N3-0170.
IntechOpen implements a robust policy to minimize and deal with instances of fraud or misconduct. As part of our general commitment to transparency and openness, and in order to maintain high scientific standards, we have a well-defined editorial policy regarding Retractions and Corrections.
",metaTitle:"Retraction and Correction Policy",metaDescription:"Retraction and Correction Policy",metaKeywords:null,canonicalURL:"/page/retraction-and-correction-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\\n\\n1. RETRACTIONS
\\n\\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\\n\\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\\n\\nPublishing of a Retraction Notice will adhere to the following guidelines:
\\n\\n1.2. REMOVALS AND CANCELLATIONS
\\n\\n2. STATEMENTS OF CONCERN
\\n\\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\\n\\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\\n\\n3. CORRECTIONS
\\n\\nA Correction will be issued by the Academic Editor when:
\\n\\n3.1. ERRATUM
\\n\\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\\n\\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n3.2. CORRIGENDUM
\\n\\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n4. FINAL REMARKS
\\n\\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\\n\\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\\n\\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
\\n\\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
\\n\\nPolicy last updated: 2017-09-11
\\n"}]'},components:[{type:"htmlEditorComponent",content:'IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\n\n1. RETRACTIONS
\n\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\n\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\n\nPublishing of a Retraction Notice will adhere to the following guidelines:
\n\n1.2. REMOVALS AND CANCELLATIONS
\n\n2. STATEMENTS OF CONCERN
\n\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\n\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\n\n3. CORRECTIONS
\n\nA Correction will be issued by the Academic Editor when:
\n\n3.1. ERRATUM
\n\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\n\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n3.2. CORRIGENDUM
\n\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n4. FINAL REMARKS
\n\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\n\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\n\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
\n\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
\n\nPolicy last updated: 2017-09-11
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Maghraby"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"8437",title:"Fusion Energy",subtitle:null,isOpenForSubmission:!1,hash:"ae4950c5b74da69a166ed0405f3f5ade",slug:"fusion-energy",bookSignature:"Aamir Shahzad",coverURL:"https://cdn.intechopen.com/books/images_new/8437.jpg",editedByType:"Edited by",editors:[{id:"288354",title:"Dr.",name:"Aamir",middleName:null,surname:"Shahzad",slug:"aamir-shahzad",fullName:"Aamir Shahzad"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6607",title:"Ion Beam Applications",subtitle:null,isOpenForSubmission:!1,hash:"53c2938c2e40ea953ca3cb4a686d348c",slug:"ion-beam-applications",bookSignature:"Ishaq Ahmad and Malik Maaza",coverURL:"https://cdn.intechopen.com/books/images_new/6607.jpg",editedByType:"Edited by",editors:[{id:"204045",title:"Dr.",name:"Ishaq",middleName:null,surname:"Ahmad",slug:"ishaq-ahmad",fullName:"Ishaq Ahmad"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6149",title:"Ionizing Radiation Effects and Applications",subtitle:null,isOpenForSubmission:!1,hash:"9d3bc531cb8e2ffbe4a436ab42b70653",slug:"ionizing-radiation-effects-and-applications",bookSignature:"Boualem Djezzar",coverURL:"https://cdn.intechopen.com/books/images_new/6149.jpg",editedByType:"Edited by",editors:[{id:"18189",title:"Prof.",name:"Boualem",middleName:null,surname:"Djezzar",slug:"boualem-djezzar",fullName:"Boualem Djezzar"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5451",title:"New Insights on Gamma Rays",subtitle:null,isOpenForSubmission:!1,hash:"0fe8c3174bbb6d68493d39220cdec7ca",slug:"new-insights-on-gamma-rays",bookSignature:"Ahmed M. Maghraby",coverURL:"https://cdn.intechopen.com/books/images_new/5451.jpg",editedByType:"Edited by",editors:[{id:"102209",title:"Dr.",name:"Ahmed M.",middleName:null,surname:"Maghraby",slug:"ahmed-m.-maghraby",fullName:"Ahmed M. Maghraby"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5094",title:"Neutron Scattering",subtitle:null,isOpenForSubmission:!1,hash:"8c7f3fac75e54e8345b01ca5cb1a4e68",slug:"neutron-scattering",bookSignature:"Waldemar Alfredo Monteiro",coverURL:"https://cdn.intechopen.com/books/images_new/5094.jpg",editedByType:"Edited by",editors:[{id:"118821",title:"Dr.",name:"Waldemar Alfredo",middleName:null,surname:"Monteiro",slug:"waldemar-alfredo-monteiro",fullName:"Waldemar Alfredo Monteiro"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1616",title:"Particle Physics",subtitle:null,isOpenForSubmission:!1,hash:"29e08be0c8877548d8d9daa55a06fe3b",slug:"particle-physics",bookSignature:"Eugene Kennedy",coverURL:"https://cdn.intechopen.com/books/images_new/1616.jpg",editedByType:"Edited by",editors:[{id:"101837",title:"Dr.",name:"Eugene",middleName:null,surname:"Kennedy",slug:"eugene-kennedy",fullName:"Eugene Kennedy"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1590",title:"Gamma Radiation",subtitle:null,isOpenForSubmission:!1,hash:"30f1336f3c9399366ea01d1f1a33f920",slug:"gamma-radiation",bookSignature:"Feriz Adrovic",coverURL:"https://cdn.intechopen.com/books/images_new/1590.jpg",editedByType:"Edited by",editors:[{id:"106756",title:"Prof.",name:"Feriz",middleName:null,surname:"Adrovic",slug:"feriz-adrovic",fullName:"Feriz Adrovic"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:8,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"32842",doi:"10.5772/34901",title:"Sterilization by Gamma Irradiation",slug:"sterilization-by-gamma-irradiation",totalDownloads:74812,totalCrossrefCites:37,totalDimensionsCites:85,abstract:null,book:{id:"1590",slug:"gamma-radiation",title:"Gamma Radiation",fullTitle:"Gamma Radiation"},signatures:"Kátia Aparecida da Silva Aquino",authors:[{id:"102109",title:"Dr.",name:"Katia",middleName:"Aparecida Da S.",surname:"Aquino",slug:"katia-aquino",fullName:"Katia Aquino"}]},{id:"58998",doi:"10.5772/intechopen.73234",title:"Ionizing Radiation-Induced Polymerization",slug:"ionizing-radiation-induced-polymerization",totalDownloads:1812,totalCrossrefCites:8,totalDimensionsCites:17,abstract:"Ionizing radiation can induce some kinds of reactions, other than polymerization, such as dimerization, oligomerization, curing, and grafting. These reactions occur through a regular radical chain causing growth of polymer by three steps, namely, initiation, propagation, and termination. To understand ionizing radiation-induced polymerization, the water radiolysis must be taken into consideration. This chapter explores the mechanism of water molecules radiolysis paying especial attention to the basic regularities of solvent radicals’ interaction with the polymer molecules for forming the crosslinked polymer. Water radiolysis is the main engine of the polymerization processes, especially the “free-radical polymerization.” The mechanisms of the free-radical polymerization and crosslinking will be discussed in detail later. Since different polymers respond differently to radiation, it is useful to quantify the response, namely in terms of crosslinking and chain scission. A parameter called the G-value is frequently used for this purpose. It represents the chemical yield of crosslinks, scissions and double bonds, etc. For the crosslinked polymer, the crosslinking density increases with increasing the radiation dose, this is reflected by the swelling degree of the polymer while being immersed in a compatible solvent. If crosslinking predominates, the crosslinking density increases and the extent of swelling decreases. If chain scission predominates, the opposite occurs. A further detailed discussion of these aspects is presented throughout this chapter.",book:{id:"6149",slug:"ionizing-radiation-effects-and-applications",title:"Ionizing Radiation Effects and Applications",fullTitle:"Ionizing Radiation Effects and Applications"},signatures:"Mohamed Mohamady Ghobashy",authors:[{id:"212371",title:"Dr.",name:"Mohamed",middleName:null,surname:"Mohamady Ghobashy",slug:"mohamed-mohamady-ghobashy",fullName:"Mohamed Mohamady Ghobashy"}]},{id:"53504",doi:"10.5772/66925",title:"Applications of Ionizing Radiation in Mutation Breeding",slug:"applications-of-ionizing-radiation-in-mutation-breeding",totalDownloads:3501,totalCrossrefCites:9,totalDimensionsCites:13,abstract:"As a predicted result of increasing population worldwide, improvements in the breeding strategies in agriculture are valued as mandatory. The natural resources are limited, and due to the natural disasters like sudden and severe abiotic stress factors, excessive floods, etc., the production capacities are changed per year. In contrast, the yield potential should be significantly increased to cope with this problem. Despite rich genetic diversity, manipulation of the cultivars through alternative techniques such as mutation breeding becomes important. Radiation is proven as an effective method as a unique method to increase the genetic variability of the species. Gamma radiation is the most preferred physical mutagen by plant breeders. Several mutant varieties have been successfully introduced into commercial production by this method. Combinational use of in vitro tissue culture and mutation breeding methods makes a significant contribution to improve new crops. Large populations and the target mutations can be easily screened and identified by new methods. Marker assisted selection and advanced techniques such as microarray, next generation sequencing methods to detect a specific mutant in a large population will help to the plant breeders to use ionizing radiation efficiently in breeding programs.",book:{id:"5451",slug:"new-insights-on-gamma-rays",title:"New Insights on Gamma Rays",fullTitle:"New Insights on Gamma Rays"},signatures:"Özge Çelik and Çimen Atak",authors:[{id:"147362",title:"Dr.",name:"Özge",middleName:null,surname:"Çelik",slug:"ozge-celik",fullName:"Özge Çelik"},{id:"147364",title:"Prof.",name:"Çimen",middleName:null,surname:"Atak",slug:"cimen-atak",fullName:"Çimen Atak"}]},{id:"32846",doi:"10.5772/36950",title:"Current Importance and Potential Use of Low Doses of Gamma Radiation in Forest Species",slug:"current-importance-and-potential-use-of-low-doses-of-gamma-radiation-in-forest-species",totalDownloads:5300,totalCrossrefCites:2,totalDimensionsCites:13,abstract:null,book:{id:"1590",slug:"gamma-radiation",title:"Gamma Radiation",fullTitle:"Gamma Radiation"},signatures:"L. G. Iglesias-Andreu, P. Octavio-Aguilar and J. Bello-Bello",authors:[{id:"110581",title:"Dr.",name:"Lourdes",middleName:null,surname:"Iglesias-Andreu",slug:"lourdes-iglesias-andreu",fullName:"Lourdes Iglesias-Andreu"}]},{id:"58410",doi:"10.5772/intechopen.72074",title:"Radiation-Induced Degradation of Organic Compounds and Radiation Technologies for Purification of Aqueous Systems",slug:"radiation-induced-degradation-of-organic-compounds-and-radiation-technologies-for-purification-of-aq",totalDownloads:1435,totalCrossrefCites:8,totalDimensionsCites:13,abstract:"Environmental application of radiation technologies is an important part of radiation processing. Radiation treatment of aqueous systems contaminated with organic compounds is a promising method of water and wastewater purification and corresponding technologies are being developed. In this chapter, the following aspects of radiation treatment process are considered: sources of contamination and major contaminants of water and wastewater; primary processes in aqueous systems initiated by ionizing radiation; principal ways of contaminant conversion as consequences of primary processes (complete mineralization of organic compounds, partial decomposition of organic molecules resulted in detoxification, decolorization, disinfection of polluted water, and improvement in biological degradation of contaminant, polymerization of monomers’ contaminants, oxidation-reduction processes, and coagulation of colloids); sources of ionizing radiation; and main equipment applied in radiation technologies of aqueous system purification.",book:{id:"6149",slug:"ionizing-radiation-effects-and-applications",title:"Ionizing Radiation Effects and Applications",fullTitle:"Ionizing Radiation Effects and Applications"},signatures:"Igor E. Makarov and Alexander V. Ponomarev",authors:[{id:"213652",title:"Dr.",name:"Igor",middleName:null,surname:"Makarov",slug:"igor-makarov",fullName:"Igor Makarov"},{id:"213657",title:"Dr.",name:"Alexander",middleName:null,surname:"Ponomarev",slug:"alexander-ponomarev",fullName:"Alexander Ponomarev"}]}],mostDownloadedChaptersLast30Days:[{id:"32842",title:"Sterilization by Gamma Irradiation",slug:"sterilization-by-gamma-irradiation",totalDownloads:74818,totalCrossrefCites:37,totalDimensionsCites:85,abstract:null,book:{id:"1590",slug:"gamma-radiation",title:"Gamma Radiation",fullTitle:"Gamma Radiation"},signatures:"Kátia Aparecida da Silva Aquino",authors:[{id:"102109",title:"Dr.",name:"Katia",middleName:"Aparecida Da S.",surname:"Aquino",slug:"katia-aquino",fullName:"Katia Aquino"}]},{id:"32837",title:"Environmental Gamma-Ray Observation in Deep Sea",slug:"environmental-gamma-ray-observation-in-deep-sea-",totalDownloads:2931,totalCrossrefCites:4,totalDimensionsCites:6,abstract:null,book:{id:"1590",slug:"gamma-radiation",title:"Gamma Radiation",fullTitle:"Gamma Radiation"},signatures:"Hidenori Kumagai, Ryoichi Iwase, Masataka Kinoshita, Hideaki Machiyama, Mutsuo Hattori and Masaharu Okano",authors:[{id:"108174",title:"Dr.",name:"Hidenori",middleName:null,surname:"Kumagai",slug:"hidenori-kumagai",fullName:"Hidenori Kumagai"},{id:"108237",title:"Dr.",name:"Masa",middleName:null,surname:"Kinoshita",slug:"masa-kinoshita",fullName:"Masa Kinoshita"},{id:"137650",title:"Dr.",name:"Ryoichi",middleName:null,surname:"Iwase",slug:"ryoichi-iwase",fullName:"Ryoichi Iwase"},{id:"137656",title:"Dr.",name:"Hideaki",middleName:null,surname:"Machiyama",slug:"hideaki-machiyama",fullName:"Hideaki Machiyama"},{id:"146918",title:"Dr.",name:"Mutsuo",middleName:null,surname:"Hattori",slug:"mutsuo-hattori",fullName:"Mutsuo Hattori"},{id:"146919",title:"Dr.",name:"Masaharu",middleName:null,surname:"Okano",slug:"masaharu-okano",fullName:"Masaharu Okano"}]},{id:"58998",title:"Ionizing Radiation-Induced Polymerization",slug:"ionizing-radiation-induced-polymerization",totalDownloads:1820,totalCrossrefCites:8,totalDimensionsCites:17,abstract:"Ionizing radiation can induce some kinds of reactions, other than polymerization, such as dimerization, oligomerization, curing, and grafting. These reactions occur through a regular radical chain causing growth of polymer by three steps, namely, initiation, propagation, and termination. To understand ionizing radiation-induced polymerization, the water radiolysis must be taken into consideration. This chapter explores the mechanism of water molecules radiolysis paying especial attention to the basic regularities of solvent radicals’ interaction with the polymer molecules for forming the crosslinked polymer. Water radiolysis is the main engine of the polymerization processes, especially the “free-radical polymerization.” The mechanisms of the free-radical polymerization and crosslinking will be discussed in detail later. Since different polymers respond differently to radiation, it is useful to quantify the response, namely in terms of crosslinking and chain scission. A parameter called the G-value is frequently used for this purpose. It represents the chemical yield of crosslinks, scissions and double bonds, etc. For the crosslinked polymer, the crosslinking density increases with increasing the radiation dose, this is reflected by the swelling degree of the polymer while being immersed in a compatible solvent. If crosslinking predominates, the crosslinking density increases and the extent of swelling decreases. If chain scission predominates, the opposite occurs. A further detailed discussion of these aspects is presented throughout this chapter.",book:{id:"6149",slug:"ionizing-radiation-effects-and-applications",title:"Ionizing Radiation Effects and Applications",fullTitle:"Ionizing Radiation Effects and Applications"},signatures:"Mohamed Mohamady Ghobashy",authors:[{id:"212371",title:"Dr.",name:"Mohamed",middleName:null,surname:"Mohamady Ghobashy",slug:"mohamed-mohamady-ghobashy",fullName:"Mohamed Mohamady Ghobashy"}]},{id:"53780",title:"Gamma-Ray Spectrometry and the Investigation of Environmental and Food Samples",slug:"gamma-ray-spectrometry-and-the-investigation-of-environmental-and-food-samples",totalDownloads:2529,totalCrossrefCites:1,totalDimensionsCites:1,abstract:"Gamma radiation consists of high‐energy photons and penetrates matter. This is an advantage for the detection of gamma rays, as gamma spectrometry does not need the elimination of the matrix. The disadvantage is the need of shielding to protect against this radiation. Gamma rays are everywhere: in the atmosphere; gamma nuclides are produced by radiation of the sun; in the Earth, the primordial radioactive nuclides thorium and uranium are sources for gamma and other radiation. The technical enrichment and use of radioisotopes led to the unscrupulously use of radioactive material and to the Cold War, with over 900 bomb tests from 1945 to 1990, combined with global fallout over the northern hemisphere. The friendly use of radiation in medicine and for the production of energy at nuclear power plants (NPPs) has caused further expositions with ionising radiation. This chapter describes in a practical manner the instrumentation for the detection of gamma radiation and some results of the use of these techniques in environmental and food investigations.",book:{id:"5451",slug:"new-insights-on-gamma-rays",title:"New Insights on Gamma Rays",fullTitle:"New Insights on Gamma Rays"},signatures:"Markus R. Zehringer",authors:[{id:"311750",title:"Dr.",name:"Markus R.",middleName:null,surname:"Zehringer",slug:"markus-r.-zehringer",fullName:"Markus R. Zehringer"}]},{id:"54118",title:"Gamma Rays from Space",slug:"gamma-rays-from-space",totalDownloads:2089,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"An overview of gamma rays from space is presented. We highlight the most powerful astrophysical explosions, known as gamma-ray bursts. The main features observed in detectors onboard satellites are indicated. In addition, we also highlight a chronological description of the efforts made to observe their high energy counterpart at ground level. Some candidates of the GeV counterpart of gamma-ray bursts, observed by Tupi telescopes, are also presented.",book:{id:"5451",slug:"new-insights-on-gamma-rays",title:"New Insights on Gamma Rays",fullTitle:"New Insights on Gamma Rays"},signatures:"Carlos Navia and Marcel Nogueira de Oliveira",authors:[{id:"189908",title:"Dr.",name:"Carlos",middleName:null,surname:"Navia",slug:"carlos-navia",fullName:"Carlos Navia"},{id:"243084",title:"MSc.",name:"Marcel",middleName:null,surname:"De Oliveira",slug:"marcel-de-oliveira",fullName:"Marcel De Oliveira"}]}],onlineFirstChaptersFilter:{topicId:"227",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82739",title:"Experimental Breeder Reactor II",slug:"experimental-breeder-reactor-ii",totalDownloads:11,totalDimensionsCites:0,doi:"10.5772/intechopen.105800",abstract:"The Experimental Breeder Reactor II (EBR-II) operated from 1964 to 1994. EBR-II was a sodium-cooled fast reactor operating at 69 MWth producing 19 MWe. Rather than using a loop approach for the coolant, EBR-II used a pool arrangement where the reactor core, primary coolant piping, and primary reactor coolant pumps were contained within the pool of sodium. Also contained within the pool was a heat exchanger where primary coolant, which is radioactive, transferred heat to secondary, nonradioactive, sodium. The nuclear power plant included a sodium boiler building where heat from the secondary sodium generated superheated steam, which was delivered to a turbine/generator for electricity production. EBR-II fuel was metallic uranium alloyed with various metals providing significant performance and safety enhancements over oxide fuel. The most significant EBR-II experiments occurred in April 1986. Relying on inherent physical properties of the reactor, two experiments were performed subjecting the reactor to loss of primary coolant flow without reactor SCRAM and loss of the secondary system heat removal without reactor SCRAM. In both experiments, the reactor experienced no damage. This chapter provides a description of the most important design features of EBR-II along with a summary of the landmark reactor safety experiments.",book:{id:"10982",title:"Nuclear Reactors - Spacecraft Propulsion, Research Reactors, and Reactor Analysis Topics",coverURL:"https://cdn.intechopen.com/books/images_new/10982.jpg"},signatures:"Chad L. Pope, Ryan Stewart and Edward Lum"},{id:"82712",title:"Idaho State University AGN-201 Low Power Teaching Reactor: An Overlooked Gem",slug:"idaho-state-university-agn-201-low-power-teaching-reactor-an-overlooked-gem",totalDownloads:10,totalDimensionsCites:0,doi:"10.5772/intechopen.105799",abstract:"A category of reactors called university research and teaching reactors, includes relatively high-power pool-type and low-power solid-core reactors. Many high-power university reactors are largely used for irradiations and isotope production. Their almost constant operation tends to impede student access. A university reactor can be particularly relevant to the university’s mission of preparing well-rounded students who have theoretical knowledge, reinforced by focused laboratory reactor experience. The solid-core Idaho State University Aerojet General Nucleonics (AGN) model 201 reactor operates at such a low power (5 W maximum) that it is not useful for isotope production activities. However, the AGN-201 reactor is well suited for teaching and research activities. The solid-core AGN-201 reactor requires no active cooling system, uses a simple shielding arrangement, and the very low operating power results in trivial burnup providing an operating lifetime exceeding many decades. It is thus worthwhile to examine the Idaho State University AGN-201 nuclear reactor more closely because it offers a wide range of research and teaching capabilities while being widely available to students.",book:{id:"10982",title:"Nuclear Reactors - Spacecraft Propulsion, Research Reactors, and Reactor Analysis Topics",coverURL:"https://cdn.intechopen.com/books/images_new/10982.jpg"},signatures:"Chad L. Pope and William Phoenix"},{id:"81424",title:"Core Reload Analysis Techniques in the Advanced Test Reactor",slug:"core-reload-analysis-techniques-in-the-advanced-test-reactor",totalDownloads:13,totalDimensionsCites:0,doi:"10.5772/intechopen.103896",abstract:"Since becoming a national user facility in 2007, the type of irradiation campaigns the Advanced Test Reactor (ATR) supports has become much more diverse and complex. In prior years, test complexity was limited by the computational ability to analyze the tests’ influence on the fuel. Large volume tests are irradiated in flux traps which are designed to receive excess neutrons from the surrounding fuel elements. Typically, fuel elements drive the test conditions, not vice versa. The computational tool, PDQ, was used for core physics analysis for decades. The PDQ code was adequate so long as the diffusion approximation between test and fuel element remained valid. This paradigm changed with the introduction of the Ki-Jang Research Reactor—Fuel Assembly Irradiation (KJRR-FAI) in 2015. The KJRR-FAI was a prototypic fuel element for the KJRR research reactor project in the Republic of Korea. The KJRR-FAI irradiation presented multiple modeling and simulation challenges for which PDQ was ill suited. To demonstrate that the KJRR-FAI could be irradiated and meet safety requirements, the modern neutron transport codes, HELIOS and MCNP, were extensively verified and validated to replace PDQ. The hybrid 3D/2D methodology devised with these codes made analysis of the ATR with KJRR-FAI possible. The KJRR-FAI was irradiated in 2015-2016.",book:{id:"10982",title:"Nuclear Reactors - Spacecraft Propulsion, Research Reactors, and Reactor Analysis Topics",coverURL:"https://cdn.intechopen.com/books/images_new/10982.jpg"},signatures:"Samuel E. Bays and Joseph W. Nielsen"},{id:"81555",title:"Nuclear Thermal Propulsion",slug:"nuclear-thermal-propulsion",totalDownloads:113,totalDimensionsCites:0,doi:"10.5772/intechopen.103895",abstract:"This chapter will cover the fundamentals of nuclear thermal propulsion systems, covering basic principles of operation and why nuclear is a superior option to chemical rockets for interplanetary travel. It will begin with a historical overview from early efforts in the early 1950s up to current interests, with respect to fuel types, core materials, and ongoing testing efforts. An overview will be provided of reactor types and design elements for reactor concepts or testing systems for nuclear thermal propulsion, followed by a discussion of nuclear thermal design concepts. A section on system design and modeling will be presented to discuss modeling and simulation of driving phenomena: neutronics, materials performance, heat transfer, and structural mechanics, solved in a tightly coupled multiphysics system. Finally, it will show the results of a coupled physics model for a conceptual design with simulation of rapid startup transients needed to maximize hydrogen efficiency.",book:{id:"10982",title:"Nuclear Reactors - Spacecraft Propulsion, Research Reactors, and Reactor Analysis Topics",coverURL:"https://cdn.intechopen.com/books/images_new/10982.jpg"},signatures:"Mark D. DeHart, Sebastian Schunert and Vincent M. Labouré"},{id:"81013",title:"Cyber-Informed Engineering for Nuclear Reactor Digital Instrumentation and Control",slug:"cyber-informed-engineering-for-nuclear-reactor-digital-instrumentation-and-control",totalDownloads:32,totalDimensionsCites:0,doi:"10.5772/intechopen.101807",abstract:"As nuclear reactors transition from analog to digital technology, the benefits of enhanced operational capabilities and improved efficiencies are potentially offset by cyber risks. Cyber-Informed Engineering (CIE) is an approach that can be used by engineers and staff to characterize and reduce new cyber risks in digital instrumentation and control systems. CIE provides guidance that can be applied throughout the entire systems engineering lifecycle, from conceptual design to decommissioning. In addition to outlining the use of CIE in nuclear reactor applications, this chapter provides a brief primer on nuclear reactor instrumentation and control and the associated cyber risks in existing light water reactors as well as the digital technology that will likely be used in future reactor designs and applications.",book:{id:"10982",title:"Nuclear Reactors - Spacecraft Propulsion, Research Reactors, and Reactor Analysis Topics",coverURL:"https://cdn.intechopen.com/books/images_new/10982.jpg"},signatures:"Shannon Eggers and Robert Anderson"},{id:"79671",title:"Fault Detection by Signal Reconstruction in Nuclear Power Plants",slug:"fault-detection-by-signal-reconstruction-in-nuclear-power-plants",totalDownloads:105,totalDimensionsCites:0,doi:"10.5772/intechopen.101276",abstract:"In this work, the recently developed auto associative bilateral kernel regression (AABKR) method for on-line condition monitoring of systems, structures, and components (SSCs) during transient process operation of a nuclear power plant (NPP) is improved. The advancement enhances the capability of reconstructing abnormal signals to the values expected in normal conditions during both transient and steady-state process operations. The modification introduced to the method is based on the adoption of two new approaches using dynamic time warping (DTW) for the identification of the time position index (the position of the nearest vector within the historical data vectors to the current on-line query measurement) used by the weighted-distance algorithm that captures temporal dependences in the data. Applications are provided to a steady-state numerical process and a case study concerning sensor signals collected from a reactor coolant system (RCS) during start-up operation of a NPP. 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Radiotherapy and Nuclear Medicine Technology has always been my aspiration and my life. As years passed I accumulated a tremendous amount of skills and knowledge in Radiotherapy and Nuclear Medicine, Conventional Radiology, Radiation Protection, Bioinformatics Technology, PACS, Image processing, clinically and lecturing that will enable me to provide a valuable service to the community as a Researcher and Consultant in this field. My method of translating this into day to day in clinical practice is non-exhaustible and my habit of exchanging knowledge and expertise with others in those fields is the code and secret of success.",institutionString:null,institution:{name:"Majmaah University",country:{name:"Saudi Arabia"}}},{id:"313277",title:"Dr.",name:"Bartłomiej",middleName:null,surname:"Płaczek",slug:"bartlomiej-placzek",fullName:"Bartłomiej Płaczek",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/313277/images/system/313277.jpg",biography:"Bartłomiej Płaczek, MSc (2002), Ph.D. (2005), Habilitation (2016), is a professor at the University of Silesia, Institute of Computer Science, Poland, and an expert from the National Centre for Research and Development. His research interests include sensor networks, smart sensors, intelligent systems, and image processing with applications in healthcare and medicine. He is the author or co-author of more than seventy papers in peer-reviewed journals and conferences as well as the co-author of several books. He serves as a reviewer for many scientific journals, international conferences, and research foundations. Since 2010, Dr. Placzek has been a reviewer of grants and projects (including EU projects) in the field of information technologies.",institutionString:"University of Silesia",institution:{name:"University of Silesia",country:{name:"Poland"}}},{id:"35000",title:"Prof.",name:"Ulrich H.P",middleName:"H.P.",surname:"Fischer",slug:"ulrich-h.p-fischer",fullName:"Ulrich H.P Fischer",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/35000/images/3052_n.jpg",biography:"Academic and Professional Background\nUlrich H. P. has Diploma and PhD degrees in Physics from the Free University Berlin, Germany. He has been working on research positions in the Heinrich-Hertz-Institute in Germany. Several international research projects has been performed with European partners from France, Netherlands, Norway and the UK. He is currently Professor of Communications Systems at the Harz University of Applied Sciences, Germany.\n\nPublications and Publishing\nHe has edited one book, a special interest book about ‘Optoelectronic Packaging’ (VDE, Berlin, Germany), and has published over 100 papers and is owner of several international patents for WDM over POF key elements.\n\nKey Research and Consulting Interests\nUlrich’s research activity has always been related to Spectroscopy and Optical Communications Technology. Specific current interests include the validation of complex instruments, and the application of VR technology to the development and testing of measurement systems. He has been reviewer for several publications of the Optical Society of America\\'s including Photonics Technology Letters and Applied Optics.\n\nPersonal Interests\nThese include motor cycling in a very relaxed manner and performing martial arts.",institutionString:null,institution:{name:"Charité",country:{name:"Germany"}}},{id:"341622",title:"Ph.D.",name:"Eduardo",middleName:null,surname:"Rojas Alvarez",slug:"eduardo-rojas-alvarez",fullName:"Eduardo Rojas Alvarez",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/341622/images/15892_n.jpg",biography:null,institutionString:null,institution:{name:"University of Cuenca",country:{name:"Ecuador"}}},{id:"215610",title:"Prof.",name:"Muhammad",middleName:null,surname:"Sarfraz",slug:"muhammad-sarfraz",fullName:"Muhammad Sarfraz",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/215610/images/system/215610.jpeg",biography:"Muhammad Sarfraz is a professor in the Department of Information Science, Kuwait University. His research interests include computer graphics, computer vision, image processing, machine learning, pattern recognition, soft computing, data science, intelligent systems, information technology, and information systems. Prof. Sarfraz has been a keynote/invited speaker on various platforms around the globe. He has advised various students for their MSc and Ph.D. theses. He has published more than 400 publications as books, journal articles, and conference papers. He is a member of various professional societies and a chair and member of the International Advisory Committees and Organizing Committees of various international conferences. Prof. Sarfraz is also an editor-in-chief and editor of various international journals.",institutionString:"Kuwait University",institution:{name:"Kuwait University",country:{name:"Kuwait"}}},{id:"32650",title:"Prof.",name:"Lukas",middleName:"Willem",surname:"Snyman",slug:"lukas-snyman",fullName:"Lukas Snyman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/32650/images/4136_n.jpg",biography:"Lukas Willem Snyman received his basic education at primary and high schools in South Africa, Eastern Cape. He enrolled at today's Nelson Metropolitan University and graduated from this university with a BSc in Physics and Mathematics, B.Sc Honors in Physics, MSc in Semiconductor Physics, and a Ph.D. in Semiconductor Physics in 1987. After his studies, he chose an academic career and devoted his energy to the teaching of physics to first, second, and third-year students. After positions as a lecturer at the University of Port Elizabeth, he accepted a position as Associate Professor at the University of Pretoria, South Africa.\r\n\r\nIn 1992, he motivates the concept of 'television and computer-based education” as means to reach large student numbers with only the best of teaching expertise and publishes an article on the concept in the SA Journal of Higher Education of 1993 (and later in 2003). The University of Pretoria subsequently approved a series of test projects on the concept with outreach to Mamelodi and Eerste Rust in 1993. In 1994, the University established a 'Unit for Telematic Education ' as a support section for multiple faculties at the University of Pretoria. In subsequent years, the concept of 'telematic education” subsequently becomes well established in academic circles in South Africa, grew in popularity, and is adopted by many universities and colleges throughout South Africa as a medium of enhancing education and training, as a method to reaching out to far out communities, and as a means to enhance study from the home environment.\r\n\r\nProfessor Snyman in subsequent years pursued research in semiconductor physics, semiconductor devices, microelectronics, and optoelectronics.\r\n\r\nIn 2000 he joined the TUT as a full professor. Here served for a period as head of the Department of Electronic Engineering. Here he makes contributions to solar energy development, microwave and optoelectronic device development, silicon photonics, as well as contributions to new mobile telecommunication systems and network planning in SA.\r\n\r\nCurrently, he teaches electronics and telecommunications at the TUT to audiences ranging from first-year students to Ph.D. level.\r\n\r\nFor his research in the field of 'Silicon Photonics” since 1990, he has published (as author and co-author) about thirty internationally reviewed articles in scientific journals, contributed to more than forty international conferences, about 25 South African provisional patents (as inventor and co-inventor), 8 PCT international patent applications until now. Of these, two USA patents applications, two European Patents, two Korean patents, and ten SA patents have been granted. A further 4 USA patents, 5 European patents, 3 Korean patents, 3 Chinese patents, and 3 Japanese patents are currently under consideration.\r\n\r\nRecently he has also published an extensive scholarly chapter in an internet open access book on 'Integrating Microphotonic Systems and MOEMS into standard Silicon CMOS Integrated circuitry”.\r\n\r\nFurthermore, Professor Snyman recently steered a new initiative at the TUT by introducing a 'Laboratory for Innovative Electronic Systems ' at the Department of Electrical Engineering. The model of this laboratory or center is to primarily combine outputs as achieved by high-level research with lower-level system development and entrepreneurship in a technical university environment. Students are allocated to projects at different levels with PhDs and Master students allocated to the generation of new knowledge and new technologies, while students at the diploma and Baccalaureus level are allocated to electronic systems development with a direct and a near application for application in industry or the commercial and public sectors in South Africa.\r\n\r\nProfessor Snyman received the WIRSAM Award of 1983 and the WIRSAM Award in 1985 in South Africa for best research papers by a young scientist at two international conferences on electron microscopy in South Africa. He subsequently received the SA Microelectronics Award for the best dissertation emanating from studies executed at a South African university in the field of Physics and Microelectronics in South Africa in 1987. In October of 2011, Professor Snyman received the prestigious Institutional Award for 'Innovator of the Year” for 2010 at the Tshwane University of Technology, South Africa. This award was based on the number of patents recognized and granted by local and international institutions as well as for his contributions concerning innovation at the TUT.",institutionString:null,institution:{name:"University of South Africa",country:{name:"South Africa"}}},{id:"317279",title:"Mr.",name:"Ali",middleName:"Usama",surname:"Syed",slug:"ali-syed",fullName:"Ali Syed",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/317279/images/16024_n.png",biography:"A creative, talented, and innovative young professional who is dedicated, well organized, and capable research fellow with two years of experience in graduate-level research, published in engineering journals and book, with related expertise in Bio-robotics, equally passionate about the aesthetics of the mechanical and electronic system, obtained expertise in the use of MS Office, MATLAB, SolidWorks, LabVIEW, Proteus, Fusion 360, having a grasp on python, C++ and assembly language, possess proven ability in acquiring research grants, previous appointments with social and educational societies with experience in administration, current affiliations with IEEE and Web of Science, a confident presenter at conferences and teacher in classrooms, able to explain complex information to audiences of all levels.",institutionString:null,institution:{name:"Air University",country:{name:"Pakistan"}}},{id:"75526",title:"Ph.D.",name:"Zihni Onur",middleName:null,surname:"Uygun",slug:"zihni-onur-uygun",fullName:"Zihni Onur Uygun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/75526/images/12_n.jpg",biography:"My undergraduate education and my Master of Science educations at Ege University and at Çanakkale Onsekiz Mart University have given me a firm foundation in Biochemistry, Analytical Chemistry, Biosensors, Bioelectronics, Physical Chemistry and Medicine. After obtaining my degree as a MSc in analytical chemistry, I started working as a research assistant in Ege University Medical Faculty in 2014. In parallel, I enrolled to the MSc program at the Department of Medical Biochemistry at Ege University to gain deeper knowledge on medical and biochemical sciences as well as clinical chemistry in 2014. In my PhD I deeply researched on biosensors and bioelectronics and finished in 2020. Now I have eleven SCI-Expanded Index published papers, 6 international book chapters, referee assignments for different SCIE journals, one international patent pending, several international awards, projects and bursaries. In parallel to my research assistant position at Ege University Medical Faculty, Department of Medical Biochemistry, in April 2016, I also founded a Start-Up Company (Denosens Biotechnology LTD) by the support of The Scientific and Technological Research Council of Turkey. Currently, I am also working as a CEO in Denosens Biotechnology. The main purposes of the company, which carries out R&D as a research center, are to develop new generation biosensors and sensors for both point-of-care diagnostics; such as glucose, lactate, cholesterol and cancer biomarker detections. My specific experimental and instrumental skills are Biochemistry, Biosensor, Analytical Chemistry, Electrochemistry, Mobile phone based point-of-care diagnostic device, POCTs and Patient interface designs, HPLC, Tandem Mass Spectrometry, Spectrophotometry, ELISA.",institutionString:null,institution:{name:"Ege University",country:{name:"Turkey"}}},{id:"267434",title:"Dr.",name:"Rohit",middleName:null,surname:"Raja",slug:"rohit-raja",fullName:"Rohit Raja",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/267434/images/system/267434.jpg",biography:"Dr. Rohit Raja received Ph.D. in Computer Science and Engineering from Dr. CVRAMAN University in 2016. His main research interest includes Face recognition and Identification, Digital Image Processing, Signal Processing, and Networking. Presently he is working as Associate Professor in IT Department, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur (CG), India. He has authored several Journal and Conference Papers. He has good Academics & Research experience in various areas of CSE and IT. He has filed and successfully published 27 Patents. He has received many time invitations to be a Guest at IEEE Conferences. He has published 100 research papers in various International/National Journals (including IEEE, Springer, etc.) and Proceedings of the reputed International/ National Conferences (including Springer and IEEE). He has been nominated to the board of editors/reviewers of many peer-reviewed and refereed Journals (including IEEE, Springer).",institutionString:"Guru Ghasidas Vishwavidyalaya",institution:{name:"Guru Ghasidas Vishwavidyalaya",country:{name:"India"}}},{id:"246502",title:"Dr.",name:"Jaya T.",middleName:"T",surname:"Varkey",slug:"jaya-t.-varkey",fullName:"Jaya T. Varkey",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246502/images/11160_n.jpg",biography:"Jaya T. Varkey, PhD, graduated with a degree in Chemistry from Cochin University of Science and Technology, Kerala, India. She obtained a PhD in Chemistry from the School of Chemical Sciences, Mahatma Gandhi University, Kerala, India, and completed a post-doctoral fellowship at the University of Minnesota, USA. She is a research guide at Mahatma Gandhi University and Associate Professor in Chemistry, St. Teresa’s College, Kochi, Kerala, India.\nDr. Varkey received a National Young Scientist award from the Indian Science Congress (1995), a UGC Research award (2016–2018), an Indian National Science Academy (INSA) Visiting Scientist award (2018–2019), and a Best Innovative Faculty award from the All India Association for Christian Higher Education (AIACHE) (2019). She Hashas received the Sr. Mary Cecil prize for best research paper three times. She was also awarded a start-up to develop a tea bag water filter. \nDr. Varkey has published two international books and twenty-seven international journal publications. She is an editorial board member for five international journals.",institutionString:"St. Teresa’s College",institution:null},{id:"250668",title:"Dr.",name:"Ali",middleName:null,surname:"Nabipour Chakoli",slug:"ali-nabipour-chakoli",fullName:"Ali Nabipour Chakoli",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/250668/images/system/250668.jpg",biography:"Academic Qualification:\r\n•\tPhD in Materials Physics and Chemistry, From: Sep. 2006, to: Sep. 2010, School of Materials Science and Engineering, Harbin Institute of Technology, Thesis: Structure and Shape Memory Effect of Functionalized MWCNTs/poly (L-lactide-co-ε-caprolactone) Nanocomposites. Supervisor: Prof. Wei Cai,\r\n•\tM.Sc in Applied Physics, From: 1996, to: 1998, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Determination of Boron in Micro alloy Steels with solid state nuclear track detectors by neutron induced auto radiography, Supervisors: Dr. M. Hosseini Ashrafi and Dr. A. Hosseini.\r\n•\tB.Sc. in Applied Physics, From: 1991, to: 1996, Faculty of Physics & Nuclear Science, Amirkabir Uni. of Technology, Tehran, Iran, Thesis: Design of shielding for Am-Be neutron sources for In Vivo neutron activation analysis, Supervisor: Dr. M. Hosseini Ashrafi.\r\n\r\nResearch Experiences:\r\n1.\tNanomaterials, Carbon Nanotubes, Graphene: Synthesis, Functionalization and Characterization,\r\n2.\tMWCNTs/Polymer Composites: Fabrication and Characterization, \r\n3.\tShape Memory Polymers, Biodegradable Polymers, ORC, Collagen,\r\n4.\tMaterials Analysis and Characterizations: TEM, SEM, XPS, FT-IR, Raman, DSC, DMA, TGA, XRD, GPC, Fluoroscopy, \r\n5.\tInteraction of Radiation with Mater, Nuclear Safety and Security, NDT(RT),\r\n6.\tRadiation Detectors, Calibration (SSDL),\r\n7.\tCompleted IAEA e-learning Courses:\r\nNuclear Security (15 Modules),\r\nNuclear Safety:\r\nTSA 2: Regulatory Protection in Occupational Exposure,\r\nTips & Tricks: Radiation Protection in Radiography,\r\nSafety and Quality in Radiotherapy,\r\nCourse on Sealed Radioactive Sources,\r\nCourse on Fundamentals of Environmental Remediation,\r\nCourse on Planning for Environmental Remediation,\r\nKnowledge Management Orientation Course,\r\nFood Irradiation - Technology, Applications and Good Practices,\r\nEmployment:\r\nFrom 2010 to now: Academic staff, Nuclear Science and Technology Research Institute, Kargar Shomali, Tehran, Iran, P.O. Box: 14395-836.\r\nFrom 1997 to 2006: Expert of Materials Analysis and Characterization. Research Center of Agriculture and Medicine. Rajaeeshahr, Karaj, Iran, P. O. Box: 31585-498.",institutionString:"Atomic Energy Organization of Iran",institution:{name:"Atomic Energy Organization of Iran",country:{name:"Iran"}}},{id:"248279",title:"Dr.",name:"Monika",middleName:"Elzbieta",surname:"Machoy",slug:"monika-machoy",fullName:"Monika Machoy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/248279/images/system/248279.jpeg",biography:"Monika Elżbieta Machoy, MD, graduated with distinction from the Faculty of Medicine and Dentistry at the Pomeranian Medical University in 2009, defended her PhD thesis with summa cum laude in 2016 and is currently employed as a researcher at the Department of Orthodontics of the Pomeranian Medical University. She expanded her professional knowledge during a one-year scholarship program at the Ernst Moritz Arndt University in Greifswald, Germany and during a three-year internship at the Technical University in Dresden, Germany. She has been a speaker at numerous orthodontic conferences, among others, American Association of Orthodontics, European Orthodontic Symposium and numerous conferences of the Polish Orthodontic Society. She conducts research focusing on the effect of orthodontic treatment on dental and periodontal tissues and the causes of pain in orthodontic patients.",institutionString:"Pomeranian Medical University",institution:{name:"Pomeranian Medical University",country:{name:"Poland"}}},{id:"252743",title:"Prof.",name:"Aswini",middleName:"Kumar",surname:"Kar",slug:"aswini-kar",fullName:"Aswini Kar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/252743/images/10381_n.jpg",biography:"uploaded in cv",institutionString:null,institution:{name:"KIIT University",country:{name:"India"}}},{id:"204256",title:"Dr.",name:"Anil",middleName:"Kumar",surname:"Kumar Sahu",slug:"anil-kumar-sahu",fullName:"Anil Kumar Sahu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204256/images/14201_n.jpg",biography:"I have nearly 11 years of research and teaching experience. I have done my master degree from University Institute of Pharmacy, Pt. Ravi Shankar Shukla University, Raipur, Chhattisgarh India. I have published 16 review and research articles in international and national journals and published 4 chapters in IntechOpen, the world’s leading publisher of Open access books. I have presented many papers at national and international conferences. I have received research award from Indian Drug Manufacturers Association in year 2015. My research interest extends from novel lymphatic drug delivery systems, oral delivery system for herbal bioactive to formulation optimization.",institutionString:null,institution:{name:"Chhattisgarh Swami Vivekanand Technical University",country:{name:"India"}}},{id:"253468",title:"Dr.",name:"Mariusz",middleName:null,surname:"Marzec",slug:"mariusz-marzec",fullName:"Mariusz Marzec",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/253468/images/system/253468.png",biography:"An assistant professor at Department of Biomedical Computer Systems, at Institute of Computer Science, Silesian University in Katowice. Scientific interests: computer analysis and processing of images, biomedical images, databases and programming languages. He is an author and co-author of scientific publications covering analysis and processing of biomedical images and development of database systems.",institutionString:"University of Silesia",institution:{name:"University of Silesia",country:{name:"Poland"}}},{id:"212432",title:"Prof.",name:"Hadi",middleName:null,surname:"Mohammadi",slug:"hadi-mohammadi",fullName:"Hadi Mohammadi",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/212432/images/system/212432.jpeg",biography:"Dr. Hadi Mohammadi is a biomedical engineer with hands-on experience in the design and development of many engineering structures and medical devices through various projects that he has been involved in over the past twenty years. Dr. Mohammadi received his BSc. and MSc. degrees in Mechanical Engineering from Sharif University of Technology, Tehran, Iran, and his PhD. degree in Biomedical Engineering (biomaterials) from the University of Western Ontario. He was a postdoctoral trainee for almost four years at University of Calgary and Harvard Medical School. He is an industry innovator having created the technology to produce lifelike synthetic platforms that can be used for the simulation of almost all cardiovascular reconstructive surgeries. He’s been heavily involved in the design and development of cardiovascular devices and technology for the past 10 years. He is currently an Assistant Professor with the University of British Colombia, Canada.",institutionString:"University of British Columbia",institution:{name:"University of British Columbia",country:{name:"Canada"}}},{id:"254463",title:"Prof.",name:"Haisheng",middleName:null,surname:"Yang",slug:"haisheng-yang",fullName:"Haisheng Yang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/254463/images/system/254463.jpeg",biography:"Haisheng Yang, Ph.D., Professor and Director of the Department of Biomedical Engineering, College of Life Science and Bioengineering, Beijing University of Technology. He received his Ph.D. degree in Mechanics/Biomechanics from Harbin Institute of Technology (jointly with University of California, Berkeley). Afterwards, he worked as a Postdoctoral Research Associate in the Purdue Musculoskeletal Biology and Mechanics Lab at the Department of Basic Medical Sciences, Purdue University, USA. He also conducted research in the Research Centre of Shriners Hospitals for Children-Canada at McGill University, Canada. Dr. Yang has over 10 years research experience in orthopaedic biomechanics and mechanobiology of bone adaptation and regeneration. He earned an award from Beijing Overseas Talents Aggregation program in 2017 and serves as Beijing Distinguished Professor.",institutionString:null,institution:{name:"Beijing University of Technology",country:{name:"China"}}},{id:"89721",title:"Dr.",name:"Mehmet",middleName:"Cuneyt",surname:"Ozmen",slug:"mehmet-ozmen",fullName:"Mehmet Ozmen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/89721/images/7289_n.jpg",biography:null,institutionString:null,institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"265335",title:"Mr.",name:"Stefan",middleName:"Radnev",surname:"Stefanov",slug:"stefan-stefanov",fullName:"Stefan Stefanov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/265335/images/7562_n.jpg",biography:null,institutionString:null,institution:{name:"Medical University Plovdiv",country:{name:"Bulgaria"}}},{id:"242893",title:"Ph.D. Student",name:"Joaquim",middleName:null,surname:"De Moura",slug:"joaquim-de-moura",fullName:"Joaquim De Moura",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/242893/images/7133_n.jpg",biography:"Joaquim de Moura received his degree in Computer Engineering in 2014 from the University of A Coruña (Spain). In 2016, he received his M.Sc degree in Computer Engineering from the same university. He is currently pursuing his Ph.D degree in Computer Science in a collaborative project between ophthalmology centers in Galicia and the University of A Coruña. His research interests include computer vision, machine learning algorithms and analysis and medical imaging processing of various kinds.",institutionString:null,institution:{name:"University of A Coruña",country:{name:"Spain"}}},{id:"294334",title:"B.Sc.",name:"Marc",middleName:null,surname:"Bruggeman",slug:"marc-bruggeman",fullName:"Marc Bruggeman",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/294334/images/8242_n.jpg",biography:"Chemical engineer graduate, with a passion for material science and specific interest in polymers - their near infinite applications intrigue me. \n\nI plan to continue my scientific career in the field of polymeric biomaterials as I am fascinated by intelligent, bioactive and biomimetic materials for use in both consumer and medical applications.",institutionString:null,institution:null},{id:"255757",title:"Dr.",name:"Igor",middleName:"Victorovich",surname:"Lakhno",slug:"igor-lakhno",fullName:"Igor Lakhno",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255757/images/system/255757.jpg",biography:"Igor Victorovich Lakhno was born in 1971 in Kharkiv (Ukraine). \nMD – 1994, Kharkiv National Medical Univesity.\nOb&Gyn; – 1997, master courses in Kharkiv Medical Academy of Postgraduate Education.\nPh.D. – 1999, Kharkiv National Medical Univesity.\nDSC – 2019, PL Shupik National Academy of Postgraduate Education \nProfessor – 2021, Department of Obstetrics and Gynecology of VN Karazin Kharkiv National University\nHead of Department – 2021, Department of Perinatology, Obstetrics and gynecology of Kharkiv Medical Academy of Postgraduate Education\nIgor Lakhno has been graduated from international training courses on reproductive medicine and family planning held at Debrecen University (Hungary) in 1997. Since 1998 Lakhno Igor has worked as an associate professor in the department of obstetrics and gynecology of VN Karazin National University and an associate professor of the perinatology, obstetrics, and gynecology department of Kharkiv Medical Academy of Postgraduate Education. Since June 2019 he’s been a professor in the department of obstetrics and gynecology of VN Karazin National University and a professor of the perinatology, obstetrics, and gynecology department. He’s affiliated with Kharkiv Medical Academy of Postgraduate Education as a Head of Department from November 2021. Igor Lakhno has participated in several international projects on fetal non-invasive electrocardiography (with Dr. J. A. Behar (Technion), Prof. D. Hoyer (Jena University), and José Alejandro Díaz Méndez (National Institute of Astrophysics, Optics, and Electronics, Mexico). He’s an author of about 200 printed works and there are 31 of them in Scopus or Web of Science databases. Igor Lakhno is a member of the Editorial Board of Reproductive Health of Woman, Emergency Medicine, and Technology Transfer Innovative Solutions in Medicine (Estonia). He is a medical Editor of “Z turbotoyu pro zhinku”. Igor Lakhno is a reviewer of the Journal of Obstetrics and Gynaecology (Taylor and Francis), British Journal of Obstetrics and Gynecology (Wiley), Informatics in Medicine Unlocked (Elsevier), The Journal of Obstetrics and Gynecology Research (Wiley), Endocrine, Metabolic & Immune Disorders-Drug Targets (Bentham Open), The Open Biomedical Engineering Journal (Bentham Open), etc. He’s defended a dissertation for a DSc degree “Pre-eclampsia: prediction, prevention, and treatment”. Three years ago Igor Lakhno has participated in a training course on innovative technologies in medical education at Lublin Medical University (Poland). Lakhno Igor has participated as a speaker in several international conferences and congresses (International Conference on Biological Oscillations April 10th-14th 2016, Lancaster, UK, The 9th conference of the European Study Group on Cardiovascular Oscillations). His main scientific interests: are obstetrics, women’s health, fetal medicine, and cardiovascular medicine. \nIgor Lakhno is a consultant at Kharkiv municipal perinatal center. He’s graduated from training courses on endoscopy in gynecology. He has 28 years of practical experience in the field.",institutionString:null,institution:null},{id:"244950",title:"Dr.",name:"Salvatore",middleName:null,surname:"Di Lauro",slug:"salvatore-di-lauro",fullName:"Salvatore Di Lauro",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0030O00002bSF1HQAW/ProfilePicture%202021-12-20%2014%3A54%3A14.482",biography:"Name:\n\tSALVATORE DI LAURO\nAddress:\n\tHospital Clínico Universitario Valladolid\nAvda Ramón y Cajal 3\n47005, Valladolid\nSpain\nPhone number: \nFax\nE-mail:\n\t+34 983420000 ext 292\n+34 983420084\nsadilauro@live.it\nDate and place of Birth:\nID Number\nMedical Licence \nLanguages\t09-05-1985. Villaricca (Italy)\n\nY1281863H\n474707061\nItalian (native language)\nSpanish (read, written, spoken)\nEnglish (read, written, spoken)\nPortuguese (read, spoken)\nFrench (read)\n\t\t\nCurrent position (title and company)\tDate (Year)\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. Private practise.\t2017-today\n\n2019-today\n\t\n\t\nEducation (High school, university and postgraduate training > 3 months)\tDate (Year)\nDegree in Medicine and Surgery. University of Neaples 'Federico II”\nResident in Opthalmology. Hospital Clinico Universitario Valladolid\nMaster in Vitreo-Retina. IOBA. University of Valladolid\nFellow of the European Board of Ophthalmology. Paris\nMaster in Research in Ophthalmology. University of Valladolid\t2003-2009\n2012-2016\n2016-2017\n2016\n2012-2013\n\t\nEmployments (company and positions)\tDate (Year)\nResident in Ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl.\nFellow in Vitreo-Retina. IOBA. University of Valladolid\nVitreo-Retinal consultant in ophthalmology. Hospital Clinico Universitario Valladolid. Sacyl. National Health System.\nVitreo-Retinal consultant in ophthalmology. Instituto Oftalmologico Recoletas. Red Hospitalaria Recoletas. \n\t2012-2016\n2016-2017\n2017-today\n\n2019-Today\n\n\n\t\nClinical Research Experience (tasks and role)\tDate (Year)\nAssociated investigator\n\n' FIS PI20/00740: DESARROLLO DE UNA CALCULADORA DE RIESGO DE\nAPARICION DE RETINOPATIA DIABETICA BASADA EN TECNICAS DE IMAGEN MULTIMODAL EN PACIENTES DIABETICOS TIPO 1. Grant by: Ministerio de Ciencia e Innovacion \n\n' (BIO/VA23/14) Estudio clínico multicéntrico y prospectivo para validar dos\nbiomarcadores ubicados en los genes p53 y MDM2 en la predicción de los resultados funcionales de la cirugía del desprendimiento de retina regmatógeno. Grant by: Gerencia Regional de Salud de la Junta de Castilla y León.\n' Estudio multicéntrico, aleatorizado, con enmascaramiento doble, en 2 grupos\nparalelos y de 52 semanas de duración para comparar la eficacia, seguridad e inmunogenicidad de SOK583A1 respecto a Eylea® en pacientes con degeneración macular neovascular asociada a la edad' (CSOK583A12301; N.EUDRA: 2019-004838-41; FASE III). Grant by Hexal AG\n\n' Estudio de fase III, aleatorizado, doble ciego, con grupos paralelos, multicéntrico para comparar la eficacia y la seguridad de QL1205 frente a Lucentis® en pacientes con degeneración macular neovascular asociada a la edad. (EUDRACT: 2018-004486-13). Grant by Qilu Pharmaceutical Co\n\n' Estudio NEUTON: Ensayo clinico en fase IV para evaluar la eficacia de aflibercept en pacientes Naive con Edema MacUlar secundario a Oclusion de Vena CenTral de la Retina (OVCR) en regimen de tratamientO iNdividualizado Treat and Extend (TAE)”, (2014-000975-21). Grant by Fundacion Retinaplus\n\n' Evaluación de la seguridad y bioactividad de anillos de tensión capsular en conejo. Proyecto Procusens. Grant by AJL, S.A.\n\n'Estudio epidemiológico, prospectivo, multicéntrico y abierto\\npara valorar la frecuencia de la conjuntivitis adenovírica diagnosticada mediante el test AdenoPlus®\\nTest en pacientes enfermos de conjuntivitis aguda”\\n. National, multicenter study. Grant by: NICOX.\n\nEuropean multicentric trial: 'Evaluation of clinical outcomes following the use of Systane Hydration in patients with dry eye”. Study Phase 4. Grant by: Alcon Labs'\n\nVLPs Injection and Activation in a Rabbit Model of Uveal Melanoma. Grant by Aura Bioscience\n\nUpdating and characterization of a rabbit model of uveal melanoma. Grant by Aura Bioscience\n\nEnsayo clínico en fase IV para evaluar las variantes genéticas de la vía del VEGF como biomarcadores de eficacia del tratamiento con aflibercept en pacientes con degeneración macular asociada a la edad (DMAE) neovascular. Estudio BIOIMAGE. IMO-AFLI-2013-01\n\nEstudio In-Eye:Ensayo clínico en fase IV, abierto, aleatorizado, de 2 brazos,\nmulticçentrico y de 12 meses de duración, para evaluar la eficacia y seguridad de un régimen de PRN flexible individualizado de 'esperar y extender' versus un régimen PRN según criterios de estabilización mediante evaluaciones mensuales de inyecciones intravítreas de ranibizumab 0,5 mg en pacientes naive con neovascularización coriodea secunaria a la degeneración macular relacionada con la edad. CP: CRFB002AES03T\n\nTREND: Estudio Fase IIIb multicéntrico, randomizado, de 12 meses de\nseguimiento con evaluador de la agudeza visual enmascarado, para evaluar la eficacia y la seguridad de ranibizumab 0.5mg en un régimen de tratar y extender comparado con un régimen mensual, en pacientes con degeneración macular neovascular asociada a la edad. CP: CRFB002A2411 Código Eudra CT:\n2013-002626-23\n\n\n\nPublications\t\n\n2021\n\n\n\n\n2015\n\n\n\n\n2021\n\n\n\n\n\n2021\n\n\n\n\n2015\n\n\n\n\n2015\n\n\n2014\n\n\n\n\n2015-16\n\n\n\n2015\n\n\n2014\n\n\n2014\n\n\n\n\n2014\n\n\n\n\n\n\n\n2014\n\nJose Carlos Pastor; Jimena Rojas; Salvador Pastor-Idoate; Salvatore Di Lauro; Lucia Gonzalez-Buendia; Santiago Delgado-Tirado. Proliferative vitreoretinopathy: A new concept of disease pathogenesis and practical\nconsequences. Progress in Retinal and Eye Research. 51, pp. 125 - 155. 03/2016. DOI: 10.1016/j.preteyeres.2015.07.005\n\n\nLabrador-Velandia S; Alonso-Alonso ML; Di Lauro S; García-Gutierrez MT; Srivastava GK; Pastor JC; Fernandez-Bueno I. Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures.Experimental Eye Research. 185, 17/05/2019. DOI: 10.1016/j.exer.2019.05.011\n\nSalvatore Di Lauro; Maria Teresa Garcia Gutierrez; Ivan Fernandez Bueno. Quantification of pigment epithelium-derived factor (PEDF) in an ex vivo coculture of retinal pigment epithelium cells and neuroretina.\nJournal of Allbiosolution. 2019. ISSN 2605-3535\n\nSonia Labrador Velandia; Salvatore Di Lauro; Alonso-Alonso ML; Tabera Bartolomé S; Srivastava GK; Pastor JC; Fernandez-Bueno I. Biocompatibility of intravitreal injection of human mesenchymal stem cells in immunocompetent rabbits. Graefe's archive for clinical and experimental ophthalmology. 256 - 1, pp. 125 - 134. 01/2018. DOI: 10.1007/s00417-017-3842-3\n\n\nSalvatore Di Lauro, David Rodriguez-Crespo, Manuel J Gayoso, Maria T Garcia-Gutierrez, J Carlos Pastor, Girish K Srivastava, Ivan Fernandez-Bueno. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Molecular Vision. 2016 - 22, pp. 243 - 253. 01/2016.\n\nSalvatore Di Lauro. Classifications for Proliferative Vitreoretinopathy ({PVR}): An Analysis of Their Use in Publications over the Last 15 Years. Journal of Ophthalmology. 2016, pp. 1 - 6. 01/2016. DOI: 10.1155/2016/7807596\n\nSalvatore Di Lauro; Rosa Maria Coco; Rosa Maria Sanabria; Enrique Rodriguez de la Rua; Jose Carlos Pastor. Loss of Visual Acuity after Successful Surgery for Macula-On Rhegmatogenous Retinal Detachment in a Prospective Multicentre Study. Journal of Ophthalmology. 2015:821864, 2015. DOI: 10.1155/2015/821864\n\nIvan Fernandez-Bueno; Salvatore Di Lauro; Ivan Alvarez; Jose Carlos Lopez; Maria Teresa Garcia-Gutierrez; Itziar Fernandez; Eva Larra; Jose Carlos Pastor. Safety and Biocompatibility of a New High-Density Polyethylene-Based\nSpherical Integrated Porous Orbital Implant: An Experimental Study in Rabbits. Journal of Ophthalmology. 2015:904096, 2015. DOI: 10.1155/2015/904096\n\nPastor JC; Pastor-Idoate S; Rodríguez-Hernandez I; Rojas J; Fernandez I; Gonzalez-Buendia L; Di Lauro S; Gonzalez-Sarmiento R. Genetics of PVR and RD. Ophthalmologica. 232 - Suppl 1, pp. 28 - 29. 2014\n\nRodriguez-Crespo D; Di Lauro S; Singh AK; Garcia-Gutierrez MT; Garrosa M; Pastor JC; Fernandez-Bueno I; Srivastava GK. Triple-layered mixed co-culture model of RPE cells with neuroretina for evaluating the neuroprotective effects of adipose-MSCs. Cell Tissue Res. 358 - 3, pp. 705 - 716. 2014.\nDOI: 10.1007/s00441-014-1987-5\n\nCarlo De Werra; Salvatore Condurro; Salvatore Tramontano; Mario Perone; Ivana Donzelli; Salvatore Di Lauro; Massimo Di Giuseppe; Rosa Di Micco; Annalisa Pascariello; Antonio Pastore; Giorgio Diamantis; Giuseppe Galloro. Hydatid disease of the liver: thirty years of surgical experience.Chirurgia italiana. 59 - 5, pp. 611 - 636.\n(Italia): 2007. ISSN 0009-4773\n\nChapters in books\n\t\n' Salvador Pastor Idoate; Salvatore Di Lauro; Jose Carlos Pastor Jimeno. PVR: Pathogenesis, Histopathology and Classification. Proliferative Vitreoretinopathy with Small Gauge Vitrectomy. Springer, 2018. ISBN 978-3-319-78445-8\nDOI: 10.1007/978-3-319-78446-5_2. \n\n' Salvatore Di Lauro; Maria Isabel Lopez Galvez. Quistes vítreos en una mujer joven. Problemas diagnósticos en patología retinocoroidea. Sociedad Española de Retina-Vitreo. 2018.\n\n' Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor Jimeno. iOCT in PVR management. OCT Applications in Opthalmology. pp. 1 - 8. INTECH, 2018. DOI: 10.5772/intechopen.78774.\n\n' Rosa Coco Martin; Salvatore Di Lauro; Salvador Pastor Idoate; Jose Carlos Pastor. amponadores, manipuladores y tinciones en la cirugía del traumatismo ocular.Trauma Ocular. Ponencia de la SEO 2018..\n\n' LOPEZ GALVEZ; DI LAURO; CRESPO. OCT angiografia y complicaciones retinianas de la diabetes. PONENCIA SEO 2021, CAPITULO 20. (España): 2021.\n\n' Múltiples desprendimientos neurosensoriales bilaterales en paciente joven. Enfermedades Degenerativas De Retina Y Coroides. SERV 04/2016. \n' González-Buendía L; Di Lauro S; Pastor-Idoate S; Pastor Jimeno JC. Vitreorretinopatía proliferante (VRP) e inflamación: LA INFLAMACIÓN in «INMUNOMODULADORES Y ANTIINFLAMATORIOS: MÁS ALLÁ DE LOS CORTICOIDES. RELACION DE PONENCIAS DE LA SOCIEDAD ESPAÑOLA DE OFTALMOLOGIA. 10/2014.",institutionString:null,institution:null},{id:"243698",title:"Dr.",name:"Xiaogang",middleName:null,surname:"Wang",slug:"xiaogang-wang",fullName:"Xiaogang Wang",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243698/images/system/243698.png",biography:"Dr. Xiaogang Wang, a faculty member of Shanxi Eye Hospital specializing in the treatment of cataract and retinal disease and a tutor for postgraduate students of Shanxi Medical University, worked in the COOL Lab as an international visiting scholar under the supervision of Dr. David Huang and Yali Jia from October 2012 through November 2013. Dr. Wang earned an MD from Shanxi Medical University and a Ph.D. from Shanghai Jiao Tong University. 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