Effect of calcium hydroxide and sodium hydroxide with different doses on the vigor of the seeds in different storage times.
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More than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\\n\\nOur breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\\n\\n“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\\n\\nAdditionally, each book published by IntechOpen contains original content and research findings.
\\n\\nWe are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
\\n\\n\\n\\n
\\n"}]',published:!0,mainMedia:null},components:[{type:"htmlEditorComponent",content:'
Simba Information has released its Open Access Book Publishing 2020 - 2024 report and has again identified IntechOpen as the world’s largest Open Access book publisher by title count.
\n\nSimba Information is a leading provider for market intelligence and forecasts in the media and publishing industry. The report, published every year, provides an overview and financial outlook for the global professional e-book publishing market.
\n\nIntechOpen, De Gruyter, and Frontiers are the largest OA book publishers by title count, with IntechOpen coming in at first place with 5,101 OA books published, a good 1,782 titles ahead of the nearest competitor.
\n\nSince the first Open Access Book Publishing report published in 2016, IntechOpen has held the top stop each year.
\n\n\n\nMore than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\n\nOur breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\n\n“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\n\nAdditionally, each book published by IntechOpen contains original content and research findings.
\n\nWe are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
\n\n\n\n
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In engineering education, laboratories represent an important academic resource as they provide practical training in addition to the fundamental theories taught in lectures. At laboratories, engineering students have the opportunity to learn how to properly use the equipment and tools with which they will be interacting later in a professional environment, and therefore, they gain practical experience and familiarize with that equipment. For this reason, engineering schools seek to provide properly equipped laboratories. However, the maintenance of the equipment and the acquisition of new machinery imply a large investment that only a limited number of public universities can afford.
The limited budgets and the large amount of students at engineering schools make insufficient the available resources at the laboratories and consequently, it is very difficult for universities to provide quality experimental training to all engineering students. Taking advantage of new communication technologies such as the Internet and computing tools such as virtual instrumentation, the available resources can be shared, developing and implementing collaborative schemes and e-learning environments that allow the access to practical training to a larger amount of students, regardless their location.
Several collaborative schemes, aimed at engineering education, have been developed in recent years for the remote execution of experiments in distance laboratories and on different engineering fields. For example, the remote laboratory system described in Ref. [1], where experiments conducted at control engineering laboratories can be remotely operated through the Internet. On the same trend, a more recent work [2] reports on the remote control of a nonlinear system as a tool for teaching several engineering subjects. Likewise, experiments with analog electronic circuits have been studied [3] with the aid of remote laboratories based on an existent and previously validated platform. This system aims the use of nonproprietary solutions in order to promote sharing among institutions; it also performs remote measurements over real instruments in an effort to make it as reliable as a hands-on laboratory.
Remote laboratories for experimental training on mechatronics have also been implemented, as the one reported in Ref. [4], where experiments using a two-degree of freedom robot and a servomotor can be carried out remotely. Other works have also reported the development of remote experiments implemented as web-based systems in other engineering fields such as fluid mechanics [5] and electrical engineering [6].
In general, remote laboratories deal with manipulation of real equipment and experiments, and although they make use of tools such as virtual instruments (VI) and e-learning environments, they should not be confused with either simulations or virtual laboratories. There are systems, such as the one reported in Ref. [7], using different approaches, more on the style of virtual laboratories, that involve the use of expensive equipment and topologies for the emulation of sophisticated laboratories, which are rarely available in public institutions.
Several computing tools have been employed to develop remote laboratories for engineering education. As an example, Matlab and Easy Java Simulations were used for the implementation of control system experiments in the work reported in Ref. [8]. However, since early works on distance laboratories [9, 10], LabVIEW® virtual instrumentation has been the primary used tool, and this software has become a powerful resource for the development of e-learning environments, particularly at the development stage.
Although much work has been done for several years in the field of remote laboratories, there are still many challenges before they can be considered a quotidian resource for teaching and training in engineering. Some of these challenges and proposed solutions have already been considered [11, 12], but there is still much work left to do.
The remote mechatronic systems presented in this chapter follow the outcomes of a previous work [13] and aim to provide practical training to a larger amount of students in a public university with engineering schools in five campuses, looking to overcome current limitations such as the lack of economic resources, the consequent insufficiency of laboratory equipment, and the limited flexibility of laboratory scheduling. Three experiments for training on different engineering fields have been implemented in a web-based learning environment so that they can be remotely operated over the Internet. The web-based system was developed using LabVIEW® virtual instrumentation, and its configuration allows multiple students to access the experiments simultaneously, making the proposed system suitable for teaching and practical training.
Traditionally, students who are physically at the laboratories are able to carry out experiments by making all the required connections of the laboratory equipment, and then they manually operate tools and machinery in order to execute experimental exercises. Frequently, they also measure and calculate some parameters so as to better understand the theoretical knowledge. In contrast, remote laboratories are implemented as web-based system, replacing manual operation of the experiments and enabling remote access by means of software, such as LabVIEW® virtual instrumentation, and additional hardware.
A particular web-based system has been developed for the implementation of several experiments in three different remote laboratories. The general characteristics of the system, namely overall structure, virtual instrumentation, and data acquisition (DAQ) system, are described in this section.
The overall outline of the developed system is shown in Figure 1. The required laboratory equipment to perform the experiment is already connected at the laboratory and thanks to the use of virtual instrumentation and additional hardware the experiment can be controlled through a computer interface and over the Internet.
Overall structure of the developed web-based system.
A laptop computer equipped with LabVIEW® software is located at the actual laboratory where the experiment is performed. This is the server computer where the virtual instruments are developed for the control of the experiment execute. As stated before, additional hardware was employed for the implementation of the system, namely a data acquisition (DAQ) board and a webcam, both connected to USB ports of the server computer, as well as signal conditioning circuits for the conversion of software instructions into the required power actions on the laboratory equipment. The webcam allows video communication with the laboratory, displaying images through the computer interface in order to monitor the experiment. Using the LabVIEW® web server, the experiment is available on the Internet, and it can be accessed from anywhere providing that the user has a computer (client computer) with an Internet connection.
In order to accomplish every required task of the experiment through the computer interface, LabVIEW® software was employed to develop a set of virtual instruments. This software uses a graphical programming language in which each node is a virtual instrument, or VI, and the lines or wires connecting the nodes determine the flow of data. A virtual instrument consists of a front panel and a block diagram. The front panel represents the virtual interface for the user, and it accommodates indicators, or output nodes displaying data, and controls, or input nodes, in which data can be read from. The block diagram contains the source programming code, linking nodes with wires according to the desired flow of data. Programming structures are also represented graphically in the form of blocks with iterations or cases. The software is able to read external data in many ways, particularly through data acquisition boards and, hence, providing an excellent platform for the monitoring of real-time signals, a feature that makes LabVIEW® a very useful tool for engineering applications.
Three different main virtual instruments were developed, each of them for the implementation of one of the proposed experiments. In general, the front panels of these virtual instruments show graphical indicators, allow the control of certain parameters, and display images in real time of the actual laboratory equipment controlled. Through these VI, each experiment can be controlled from the laptop computer, allowing the same practical procedures that are conventionally made by hand, e.g., pushing start/stop buttons, measuring voltages, noticing alert signals, and registering output data. Additionally, virtual instrumentation allows operations that are not easy to perform by hand, such as obtaining graphical information in real time, registering data directly into datasheet files, or recording videos of the actual experiments. It is also possible to develop tutorials using virtual instrumentation, in order to train the users in the procedures of the experiments, before they can work with the real equipment.
The data acquisition system comprising the DAQ board and the signal conditioning circuits is necessary to acquire signals sent by the laboratory equipment to the computer and to drive the actual control of the equipment after interpreting software instructions.
In order to carry out the data acquisition process, a 16-bit National Instruments multifunction DAQ board was employed for the implementation of each of the experiments, and owing to the fact that this board does not support the electric current values sent by the laboratory equipment nor it sources the required current for driving the control of the experiments, different signal conditioning circuits connected to the inputs and outputs of the DAQ board were used for each of the experiments, according to the needs. In general, these circuits were used to amplify the electrical signals sent by the digital outputs of the DAQ board and to protect this board from high voltages when receiving the electrical signals sent by the laboratory equipment. The DAQ Assistant Express VI was used in LabVIEW® software to configure the digital and/or analog ports of the DAQ board, as it was required by each experiment. Further explanation of required circuitry and DAQ board set-up is given below.
Programming virtual instruments only to control the experiments, using a computer near to the equipment in the laboratory, may have some advantages, as the ones mentioned in Section 2.2. Nevertheless, the relevant feature of the developed systems reported in this work is their ability to be accessed from anywhere through the Internet.
The LabVIEW® web server was employed in order to remotely access, over the Internet, the developed virtual environments. Using this server, the front panel of the virtual instrument can be accessed in real time and simultaneously from different locations. However, even though multiple students can access the front panel at the same time, only one of them can control the front panel and perform the experiment at a time. This is one of the most important features of the system since this allows teachers or instructors to carry out demonstrations of the experiments in real time, while the students can monitor the experiment from their own computer. This characteristic makes the system suitable as a teaching aid, in addition to its training purposes.
How remote engineering laboratories have become a powerful tool for teaching and learning has been noticed since reviewing the perception of students about the remote operation of experiments reported in Ref. [14], and it has also been shown in Ref. [15] that remote laboratories do provide educational benefits, when comparing the remote approach with the traditional hands-on laboratories.
The procedure for the configuration of the Web server and the creation of a Web site where the virtual instruments with the laboratory experiments can be accessed remotely is explained in the following paragraphs.
Before students can access the front panel and remotely control the experiment, a proper configuration of the LabVIEW® server is required. In this configuration, the Web server needs to be enabled; then, it is necessary to determine which HTTP port will be used to access the virtual instrument and who will have access to it. Once this configuration has been done, the next step is the publication of the virtual instrument in a web page by creating a HTML file. For this aim, the web publishing tool, available within LabVIEW® software, was used. Using this tool, the URL of the web page is provided with the IP address of the server computer. This URL is the one used to access the front panel from a web browser.
For the developed system, the port HTTP 80 was used since the server computer runs Windows operating system. The browser access was configured in order to enable access to the front panel to all users, i.e., from any IP address, allowing them to view and control the experiments. An important part of this configuration is the control time limit; with this option in can be specified how long a student can remotely control the front panel until that control is granted to another student who is requesting or has previously booked the control of the virtual instrument.
For the creation of the HTML file, the virtual instrument to be published was selected and the option Embedded was chosen. Here, the option Enable IMAQ Support must be selected as this allows the acquisition of images required by the virtual instruments. Besides, the option Request control when connection is established was also enabled so that when a student access the front panel through a web browser, the control of the virtual instrument will be automatically requested. Then, the name of the HTML file is given, and it is saved. Finally, the URL is provided with the name of the file and the IP address of the computer.
As stated before, several students can view remotely the front panel of the virtual instrument, but only one of them at a time can have the control of the experiment. LabVIEW® software does not need to be installed at the client computer in order to have access to the front panel through a web browser. Instead, the applications LabVIEW Run-Time Engine and Vision Run-Time Engine, from the same version of the LabVIEW® software running the virtual instruments, are necessary.
When a student accesses the front panel via the Internet, the control of the panel is requested. However, if another student is controlling the experiment at that time, the server will queue the request and the control of the experiment will be granted to the next student on the queue list, only when the student currently having the control decides to release that control or when the time limit established is reached. To release the control, students must right-click anywhere on the front panel and select the option Release Control of VI. If a student who already released the control wants to have it again, the control must be requested by right-clicking and selecting Request Control of VI. Only the user at the server computer, who could be the teacher or instructor, is able to regain the control of the front panel at any time and he or she can also check the queue list of users.
Three different experiments were implemented in the web-based system developed. The experiments proposed are the control of an electropneumatic system, the control of AC motors, and the manipulation of residential electrical circuits. The first one is performed at the industrial automation laboratory of the main campus, whereas the other two are conducted at the electric machines laboratory. A laptop computer, a DAQ board, and a webcam were used for each of the experiments. The laboratory equipment and additional hardware required for each experiment are permanently installed at the laboratories of the main campus, but they can be accessed by students from campuses in other cities, sharing the available resources.
The first of the experiments operated from the web-based environment is the control of an electropneumatic system. The experiment represents the automatic operation of an industrial process for stamping of parts, and it is aimed at students taking an undergraduate course on industrial automation.
Pneumatic equipment is employed to perform this experiment, and for the control of the process, an electropneumatic circuit was designed and connected using double-acting cylinders, electrically actuated valves, and proximity sensors. The motion sequence followed by the cylinders executes the stamping process. Proximity sensors are used to detect the position (retracted of extended) of the cylinders, and valves provide compressed air to the cylinders according to the programming code.
The front panel of the virtual instrument developed for the programmed control of this experiment is shown in Figure 2, as seen from the web browser. This virtual interface consists of different buttons that allow students to control several parameters such as the number of parts to be stamped, the drying time for each part, and the activation of the air supply. Other buttons are used to start or stop the stamping process. Indicative LEDs are also placed in this panel to show which proximity sensors are activated, and graphical indicators show an animation of the stamping process. Real-time views of the actual laboratory equipment controlled are displayed at the top right corner.
Front panel of the main virtual instrument for the control of the electropneumatic system [16].
The block diagram, shown in Figure 3, contains the programming code, in the LabVIEW® graphical programming language, called “G,” of the main virtual instrument. It consists of graphical icons that are wired to determine the flow of data, and for this application, it is composed of global variables and three subroutines (known as subVIs) whose functions are the control of the system, the image acquisition, and the virtual connections of the system. Global variables pass data among the subVIs and the main virtual instrument when they are running at the same time.
Block diagram of the main virtual instrument for the control of the electropneumatic system [16].
For this experiment, only digital input and output ports of the DAQ board were used. The digital inputs acquire signals sent by the proximity sensors in the electropneumatic system, whereas the digital output ports generate the signals sent to the electrically actuated valves. Communication of input and output signals, however, is not direct because the DAQ board handles only low-voltage digital signals, while the components in the electropneumatic system work with higher voltage levels. For that reason, signal conditioning circuits, mounted on a breadboard, were placed between the control section and the laboratory equipment in order to amplify the electrical signals sent by the digital outputs of the DAQ board and then drive the current needed by the valves to be activated. Likewise, another circuit was used to protect the equipment when acquiring the current values from the sensors. Further details of the remote operation of this experiment are described in Ref. [16].
The second experiment implemented in a web-based laboratory is the control of two AC motors. In this experiment, two three-phase squirrel-cage induction motors can be controlled in three different ways, i.e., manually (using a virtual button), automatically, and sequentially. Students attending an undergraduate course on AC motors are the more likely to perform this experiment.
Figure 4 shows the front panel of the virtual instrument developed in LabVIEW® for the control of this experiment. Two graphical displays for voltage and current waveforms and several numeric indicators are placed in the front panel. These indicators provide useful information for students who traditionally need to measure and/or calculate parameters such as the voltage, current and real, apparent and reactive power for each phase of the tested motors. They can also visualize waveforms and phase sequence without connecting additional equipment such as oscilloscopes. For the starting of the induction motors, different buttons were placed in the pages of a tab control, according to the type of control that the students wish to perform. A camera shows the operation of the controlled motors through a real-time video image. There is also a stop button available, and all the actual equipment can be monitored in real time through the virtual interface.
Front panel of the main virtual instrument developed for the control of two AC induction motors [17].
Three different signal conditioning circuits were required for this experiment: one for the conditioning of the current signals acquired, a second one for the conditioning of the voltage signals acquired, and a third one for the amplification of the signals sent in order to start and stop the motors. In the case of the voltage and current signals, these must be reduced to the proper values supported by the DAQ board. The electrical signals required to start/stop the motors were sent by two different digital ports of the DAQ board, while six analog ports of the board were employed for the acquisition of the current and voltage signals. Detailed description of the implementation of this experiment can be found in the work reported in Ref. [17].
The third experiment is directed to students of an electrical engineering program for training on residential electrical wiring and circuits. This experiment represents the electrical circuits installed around a house to deliver electricity for the lights and other appliances, and it allows turning on/off and monitoring the electrical circuits.
The proposed electrical wiring for a home consists of two separate circuits, both of them connected to the mains. The laboratory equipment employed for this experiment, namely cables, fluorescent and incandescent lamps, switches and other loads such as a domestic and an industrial ventilator were connected to a board already available for training on wiring at the laboratory.
The front panel of the developed virtual instrument for the control of this experiment is shown in Figure 5. This interface contains the controls and indicators used for turning on/off the circuits and for monitoring the electrical parameters. The manual (using a virtual button) or automatic control of the circuits can be chosen. If the automatic form is selected, time and date for turning on and off the circuits must be specified. Voltage and current values of each circuit are displayed graphically and numerically. The values of active power and reactive power, together with the power factor, are also shown, and LEDs are used to indicate whether the circuits are turned on or not. Last but not least, images of the real equipment are displayed in real time through this virtual interface.
Front panel of the virtual instrument for the control and monitoring of residential electrical circuits [18].
The graphical programming code for this virtual instrument consists of five while loop structures. These structures execute the following functions: two of them perform the turning on/off of the circuits, a third one is for the image acquisition, another one for the acquisition of the electrical signals, namely current and voltage of the circuits, and the last one is for changing the background color of the front panel.
Four analog input ports of the NI-USB 6211 DAQ board were used to acquire voltage and current signals of the two circuits, while only two digital output ports of the same DAQ board were employed to generate the signals sent in order to turn on (or off) those circuits. As in the previous ones, signal conditioning circuits were also necessary for the implementation of this experiment. Further description of the experiment and the virtual environment is described in Ref. [18].
For the implementation of the proposed experiments in the web-based system developed, the equipment and components required for each experiment were connected at the corresponding laboratory. For each experiment, a NI-USB 6211 multifunctional DAQ board and a webcam were connected to a laptop computer equipped with LabVIEW® software and placed at the laboratories, as well as the required signal conditioning circuits. Figure 6 shows the whole experimental setup in the laboratory for the remote control of AC motors. Figure 7 shows the components, equipment, laptop computer, and power interfaces for the remote experiment on residential electrical circuits. Finally, the experimental setup for the practical exercises in the electropneumatic laboratory is shown in Figure 8.
Experimental setup for the control of AC motors [17].
Experimental setup for the manipulation of residential electrical circuits [18].
Experimental setup for the control of an electropneumatic system [16].
Perhaps, the most important part of the project was the evaluation of these mechatronic systems when they were tested by engineering students. However, a complete evaluation of remote laboratories should cover many different aspects, from engineering and technical issues to the educational and pedagogical concerns. To this aim, standards have been defined and some studies have been reported, such as the one in Ref. [19], which have tried to cover at least part of the quality properties of remote laboratories for teaching and training in engineering.
In the case of the remote laboratories described in this chapter, the virtual instruments developed for the implementation of the experiments in the web-based system were evaluated locally, at a first stage, and they worked as expected performing every required task. After this validation stage of the virtual environments, the experiments were performed over the Internet by a sample of students of the main campus in order to evaluate the remote operation of the systems. This evaluation stage was conducted in two different ways: first, by one student at a time and then simultaneously from different sites.
Students expressed their experiences when using this remote approach for experimental training and positive feedback was obtained, showing how the remote operation of the proposed experiments is a useful learning tool not only for practical experimentation but also for teaching in the engineering fields. The students were particularly satisfied with the virtual interfaces since, as they suggested, they are easy to use and they also liked the simultaneous operation of the system as a teaching aid because, as they expressed, it is possible to remotely follow the experiments while the professor performs the demonstrations.
A web-based system for the remote operation of laboratory experiments has been presented. Three mechatronics experiments for practical training on different engineering fields were implemented in the web-based system developed, and they can be remotely performed from anywhere and at any time. The experiments are as follows: the control of two three-phase squirrel-cage induction motors, the manipulation of residential electrical circuits, and the control of an electropneumatic system. For the implementation of these remote laboratories, a virtual environment for each of the experiments was developed using LabVIEW® virtual instrumentation, and the required laboratory equipment for the execution of the experiments was connected at the laboratories, together with additional hardware for the interfacing between the virtual instruments in the computers and the components and equipment in the laboratories. The experiments can be monitored in real time over the Internet and through the computer interface.
Remote laboratories are highly beneficial for engineering education. Making the experiments available over the web, students have access to practical training without time or location restrictions. Remote operation of the laboratories makes sharing the available resources within different campuses of one university possible, and it also allows the establishment of collaborative schemes between institutions. In particular, engineering schools in developing countries find this approach highly advantageous because it permits the use of local resources, which are often very limited, making them accessible through the Internet to a much higher number of students located in other campuses. Furthermore, under the establishment of proper arrangements, engineering students in developing countries can obtain access to laboratories in first world institutions, improving so the global levels of education, in accordance with the Sustainable Development Goals of the United Nations [20]. In any case, remote sharing of engineering laboratories for training and teaching among different institutions will impact by increasing the practical activities of their students, and therefore improving their learning goals, even if there are many differences between systems and protocols in universities and restrictions from one country to another, as remarked in Ref. [21].
Another important strength of the system lies in its characteristic of being accessible by several students at the same time, allowing it to be used as a teaching aid for the demonstration in real time of the experiments. In this way, the knowledge and experience of an instructor can benefit not only the local students as it occurs in conventional laboratory schemes.
A management system for the registration and authentication of students to remotely access the experiments has been previously proposed [22], and it is expected to be implemented soon with each of the remote laboratories presented. This web access system will allow students to register and schedule the usage of the experiments in order to perform the experiments by their own.
As commented before, the evaluation of the web-based laboratories extends to several different areas, and it is still in progress. At a first stage, the system was evaluated by a sample of students of the main campus in order to obtain preliminary results, but the remote laboratories are expected to be implemented as complementary exercises for different undergraduate courses offered by the schools of engineering in different campuses. At the end of each term, a poll is conducted in order to obtain feedback to determine educational achievements and usefulness of the system so as to improve its performance.
Further work is focused on the implementation in the web-based system developed of new mechatronic experiments for engineering education aimed at different fields such as fluid mechanics, electronics, and civil engineering, improving the proposed system by adding new characteristics such as audio communication and other features currently typical of the Internet of things.
Fruit trees play an important role in human nutrition; and among these highlights, the papaya, this is a crop of tropical climate, widely appreciated for being one of the few fruit that provide continuous production throughout the year after the start of fruiting, to possess fruits with a high nutritional value, and to achieve high yields; generating good income to the families dedicated to their cultivation, due to the high prices that reaches in the market [1].
\nCarica papaya Linn belonging to family Caricaceae is known as papaya in France, United Kingdom, Mexico, Cuba, etc., papita in India, tree melon in Holland, paw paw in Australia and United Kingdom, and mamao in Brazil. The plant is native of tropical America. The properties of papaya fruit and other parts of the plant are also well known in traditional system of medicine. During the last few decades, considerable progress has been achieved regarding the biological activity and medicinal application of papaya and now it is considered as valuable nutraceutical fruit plant [2].
\nThe world production of papaya occurs in more than 60 countries, according to FAO, for the year 2010, a production of 11,568,346 tons of the fruit was registered, with the main producing countries of the highest to lowest volume: India, Brazil, Indonesia, Nigeria, Mexico, Ethiopia, Colombia, Thailand, and Guatemala [3].
\nAs a crop is an important source of employment, has a good yield, earliness to enter into production, and guarantees staggered crops throughout the year. Despite all these advantages, it does not reach the maximum productive potential, this is due in large measure to the problematic that it manifests in terms of the quality of seed [4]. Is consider one of the tropical fruits more appreciated for fresh consumption and for industrialization [5]. In Mexico like in Cuba, Maradol variety is the more cultivated, is a Cuban variety.
\nDifferent varieties of papaya are commercially propagated through seed an easy management and low cost, without taking into account the heterogeneity caused by crossed polinization [5].
\nIn the sowing of papaya, it is best to use freshly harvested seeds, because while increasing the storage time of the seed, the germination rate decreases [6]. The majority part of the papaya sowing is done with stored dry seeds, and in this condition, the seed germination is erratic, asynchronous, slow, and incomplete [7], which diminishes the germination percent [5]. The desiccation produces stress in the papaya seeds when the moisture content lowers to 8.0% [8], being the cause of the seed dormancy or metabolic quiescence [9, 10]. In Taiwan, the papaya industry is limited by seed germination rates [11]. This is attributed to the presence of inhibitors as the phenolic compounds in the sarcotesta and seed coat [12, 13], and in some cases, the seeds lack embryos [6].
\nThe seed is enclosed in a gelatinous sarcotesta (aril or seed coat), which is formed from the outer integument [14]. The sarcotesta can delay germination, and also dormancy is observed in seeds from which the aril has been removed [15].
\nPapaya, like many plants, presents as one of its main problems in its reproduction, the dormancy of the seed which influences the quality of it; because by reaching its maximum point of maturity, it initiates a period of latency produced by internal and external factors. It is normally interrupted when the natural conditions suitable for germination are present or when treatments are used that help to propitiate these ideal conditions and increase the percentages of germination [16], but in vitro conditions favored germination of papaya more than in vivo environment [17].
\nThe seed of papaya is characterized by being bitegumented, since the internal tegument originates the tegumen and the external one to the testa, which is multiplicative up to 60 layers and 3 distinctive strata: endotesta, mesotesta, and exotesta (sarcotesta). This last one of semipermeable consistency, high humidity and concentrate phenolic compounds that, as a whole, induce latency. This causes the inhibition of fluid and gas exchange, delayed dehydration and colonization of pathogenic microorganisms. In [18] are mentioned others researchers who investigated this problem [19, 20].
\nOne of the ways to break the latency of the seeds and make them have a good quality overall, increasing the percentages of germination is using the different methods of scarification.
\nThe methods of scarification include physical, mechanical, and biological treatments such as dry heat, the rupture of the testa, the soaking in water, and chemical solutions that promote the germination of the seeds, where any treatment that destroys or reduces the impermeability is called scarification, so in some cases, it is only enough to destroy a single point of the cover to produce the imbibitions and exchange of gases and thus initiate the germination [21].
\nApparently, latency is a survival mechanism in the presence of certain climatic conditions: very low temperatures, alternations of dry and humid times, and desert climates. The exact causes of the latency phenomenon are unknown, and on the other hand, when the latency is due to testa conditions, the lethargy ends at the moment that it cracks or weakens by mechanical or chemical actions or by effect of the environment [22, 23].
\nDifferent seed treatments to promote germination and to reduce germination time are mentioned in [24], sowing seeds and at warm temperatures, exposing dry seeds to 10°C prior to sowing, drying seeds and soaking seeds in distilled water, potassium nitrate, thiourea, sodium thiosulfate, tannic acid or ferulic acid. The same authors described contradictory results using gibberellins on papaya seed germination. But in your research, they demonstrated that dehydration to 5.3% or 6.9% and 6.8% moisture content, followed by exposure to subzero temperatures and treatment with GA3, were the most favorable combined treatments to enhance papaya seed germination.
\nThe used of smoke water on seed germination and seedling growth of papaya, cultivar Tainung No. 2 consistently and significantly increased the percentage of nitrogen in roots and shoots and significantly increased the percentage of magnesium in shoots. In these experiments, smoke-water showed potent germination promotion at low concentrations and promoted multiple growth attributes such as chlorophyll content and seedling vigor index at all concentrations in papaya seedling production [11].
\nPregerminative treatments are used to break the latency status of the seeds. In [25] are mentioned stratification and scarification. Scarification is any process that breaks, scratches, mechanically alters or softens the covers of the seeds to make them permeable to water and gases.
\nSeed scarification methods have been developed and modified over time to make these more practical and effective. Important methods of seed scarification include heat, freeze–thaw, mechanical, and acid scarification [16].
\nIn the scientific literature, some types of scarification are described, such as mechanical [26, 27, 28], physical [29, 30, 31], chemical [32, 33], and biological. Mechanical, physical, and biological scarification have disadvantages in relation to chemical scarification, because they require more time, are laborious and inadequate to condition large quantities of seed; while chemical scarification still requires more research [18], especially with calcium hydroxide.
\nThere are chemical substances used to scarify seeds, among the most used are the sulfuric acid [34], sodium hydroxide, and hydrochloric acid [7, 18, 20, 31]. The positive effect of the use of NaOH in the benefit of papaya seed is that simulates natural degradation of sarcotesta and improves the conditions of the seed, so it is a viable alternative for use in conditioning seed [18]. Other results have been demonstrated that the combination of NaOH treatment and stratification is an effective practice to break Iris lactea var. chinensis seeds dormancy and improve germination percentage [35].
\nChemistry scarification is considered as one of the most effective scarification methods used for seed scarification. Sulfur acid is the most popular and effective chemical product for acid scarification. The effectiveness of acid scarification depends on concentration of acid duration of scarification and species and cultivars used [16, 36].
\nTraditionally, it has been used to separate the mucilage from the papaya ferment the seeds in water at different time intervals and the sunny one for 2 or 3 h [37].
\nThe objective of the research was to evaluate the scarifying effect of calcium hydroxide on the germination and vigor quality of papaya seeds, Maradol variety.
\nThe experiment was carried out in the Laboratory of Seed Test of the plant of Benefit Manuel Espinosa Ramírez of the business unit of seeds base Granma, belonging to the company producer and marketer of seeds, using seed of papaya ‘Red Maradol’, collected in areas of the Experimental Station Jucaibama of the Agricultural Research Institute Jorge Dimitrov, Bayamo, Granma province.
\nThe sample consisted of 60 randomly selected fruits of hermaphroditic plants in a commercial production lot of approximately 1 ha (2222 plants), showing commercial maturity (two strips), of homogeneous size (±2 kg). The seeds were extracted, and the batch was homogenized; 200 g of fresh dough were deposited with 500 ml plastic flasks representing each experimental unit.
\nThe treatments were composed of the solution of calcium hydroxide (CaOH2) at three doses (60, 80, and 120 g l-1 of water) by dipping the seed for a period of 24 h, the standardized sodium hydroxide (NaOH) at 25% with a 15-min immersion time, for a total of four treatments plus the control and six replicates. The control consisted in fermenting in running water the seed for 24 h.
\nTo eliminate the sarcotesta (aril), the seeds were rubbed between two jute cloths where the time needed to remove all the aril of the material was evaluated, being the optimal time to use of 15 s for each treatment given the amount of material to process. Immediately, they were rinsed three times with running water, spread over a sieve in the shade and at room temperature (28 ± 1°C) for drying for 48 h.
\nThe physical quality of the seed was determined by the effectiveness of the product, physical appearance, and mechanical damage within 3 days of the treatment, compared to the control; the physiological quality was determined by the germination percentage 7 days after sowing (vigor), being valued by the germination rate [38], and 28 days for final germination (as indicated in the germination standard, with the method in sand). The seed was soaked for a term of 24 h and placed in previously disinfested aluminum trays at a temperature of 100°C.
\nThe incubation was carried out in the germinating chamber Paul Polikeit, Model HALLE S. A, with 80% of relative humidity, 40 ± 2°C of temperature and natural light; for the sanitary quality, it was determined by assembling all the treatments with the method between paper (BP), evaluating by observing the evidences of the development of microflora on the seed, during the germination test.
\nTo carry out this test, three repetitions of 25 seeds were used by extraction, it was put to incubated in water the seeds for a time of 24 h, after the time elapsed, each seed was sectioned in longitudinal form leaving the cotyledons visible placed in culture tubes wrapped with aluminum foil adding a solution of 2, 3.5-triphenyl chloride tetrazolium to 1%, and the tubes were placed in an incubator at 35 ± 1°C for a time of 2 h.
\nThe experimental design used was completely randomized with bifactorial arrangement and six replicates. The variables assessed were vigor (vigor, 120, 240, and 360 days of conservation at 4–8°C, in percent); germination (germination, 120, 240, and 360 days of conservation at 4–8°C, in percent); the time needed to eliminate aril (s), mechanical damage, MD (%), and abnormal plants, AP (%), according to ISTA Methodology [38].
\nThe data for each measured variable were statistically processed to check compliance with the normal distribution of the data (Kolmogorov-Smirnov test) and the homogeneity of the variances (Bartlett test). These two premises of the analysis of variance were not met, even after testing several data transformation equations, so we proceeded to the application of nonparametric variance analysis through Kruskal-Wallis, to demonstrate the existence or not of variability between treatments with a probability level of 0.05. The averages (aver.) of the treatments, the standard deviation (sd) of the mean, and the significance are shown. The multiple comparisons between treatments were made through the differences between the averages of the ranges [39].
\nWe also performed analysis of partial correlations between variables with the use of Spearman correlating coefficient, with the aim of determining the existence of linear relationship between selected variables. Those variables that could have a direct or inverse relationship were selected in relation to the vigor and germination in their different times used as the aril, the mechanical damages, and the percentage of abnormal seeds.
\nStatistical processing was carried out with the use of statistical packages MINITAB 13 [40] for the test of homogeneity of variances and Infostat 2017 [41] for the rest of the statistical analyses.
\nSignificant differences were found between the different treatments in the vigor of the seeds (Table 1). The highest level of vigor of the seed was due to the use of calcium hydroxide, more than sodium hydroxide and fermentation. This tendency was maintained during the different storage times of the seeds at a constant temperature. Significant although not shown statistically, there is evidence of a decrease in the seeds vigor for all the evaluated treatments by increasing the conservation time of papaya seeds, which suggests that it is more efficient to apply calcium hydroxide in order to improve the response of papaya seeds with a minimum storage time.
\nVigor (%) | \n|||||
---|---|---|---|---|---|
Compound | \nDose | \n0 days | \n120 days | \n240 days | \n360 days | \n
Aver. ± SD | \nAver. ± SD | \nAver. ± SD | \nAver. ± SD | \n||
Ca(OH)2 | \n60 g l−1 | \n80.7ab ± 2.8 | \n83.7a ± 2.7 | \n77.3b ± 2.3 | \n71.7ab ± 5.7 | \n
Ca(OH)2 | \n80 g l−1 | \n81.7ab ± 1.6 | \n81.2ab ± 1.2 | \n79.3ab ± 2.3 | \n60.0bc ± 6.8 | \n
Ca(OH)2 | \n100 g l−1 | \n89.3a ± 1.2 | \n82.3a ± 2.2 | \n82.3a ± 1.7 | \n75.7a ± 3.6 | \n
NaOH | \n25 g l−1 | \n50.3c ± 4.2 | \n42.7c ± 3.7 | \n26.5c ± 3.4 | \n6.7c ± 0.5 | \n
Fermentation \n | \n75.3bc ± 1.9 | \n75.8bc ± 3.4 | \n73.5bc ± 3.5 | \n71.7ab ± 4.8 | \n
Effect of calcium hydroxide and sodium hydroxide with different doses on the vigor of the seeds in different storage times.
Different letters indicate significant differences to p ≤ 0.05 through the differences between the average of the ranges.
The variability in the response of calcium hydroxide could be a consequence of the fact that some seeds within the same batch have a more persistent dormancy than others and that small and large seeds can be found in the same batch [42].
\nSodium hydroxide reached lower percentages, even less than 60%, which is the minimum value established for Cuba for this crop [43, 44].
\nThe physiological quality of the papaya seed is characterized by a high sensitivity to several factors with respect to germination and vigor, considering that there are integrating elements of great importance at the plantation level [45].
\nFor the germination (Table 2), the application of calcium hydroxide obtained the best results, with percentages over the 80% in comparison with the rest of the treatments. Germination was more affected when the seeds were treated with sodium hydroxide and to the extent that the storage time of the seed was increased. Like the vigor, the germination percentage decreases in the treatments when the conservation time increased.
\nGermination (%) | \n|||||
---|---|---|---|---|---|
Compound | \nDose | \n0 days | \n120 days | \n240 days | \n360 days | \n
Aver. ± SD | \nAver. ± SD | \nAver. ± SD | \nAver. ± SD | \n||
Ca(OH)2 | \n60 g l−1 | \n88.0ab ± 2.18 | \n87.3b ± 1.6 | \n80.2bc ± 1.73 | \n89.3ab ± 6.6 | \n
Ca(OH)2 | \n80 g l−1 | \n89.0ab ± 1.9 | \n89.0ab ± 1.6 | \n81.8ab ± 1.2 | \n70.5bc ± 8.2 | \n
Ca(OH)2 | \n100 g l−1 | \n94.0a ± 2.9 | \n92.3a ± 2.0 | \n85.3a ± 1.8 | \n88.8a ± 4.3 | \n
NaOH | \n25 g l−1 | \n65.3c ± 4.2 | \n56.0c ± 3.2 | \n42.0d ± 3.0 | \n15.5c ± 3.1 | \n
Fermentation \n | \n83.7bc ± 2.3 | \n83.7bc ± 2.7 | \n76.3cd ± 2.9 | \n84.3ab ± 6.08 | \n
Effect of calcium hydroxide and sodium hydroxide with different doses in germination percentage of seeds.
Different letters indicate significant differences to p ≤ 0.05 through the differences between the average of the ranges.
Physiologically, these results could be interpreted as a sequence of events of deterioration that begins with problems of functionality in the seminal membranes, which causes an excessive flow of cellular constituents, evidenced this by the high absorbance values and consequent loss of metabolites, the magnitude of which can restrict the germinative process [46].
\nCold stored papaya seeds maintained significantly higher germination and better seedling vigor than the room stored seeds. With the increase in the duration of storage seed germination decreased after 20 mo. at room temperature, it declined marginally during the same period when kept in cold storage. Irrespective of the storage conditions, seeds kept in sealed polythene bags or plastic bottles had better germination and seedling vigor than those on paper and cloth bags. Shoot length and dry weight decreased significantly with the increase in the duration of storage. Viability of papaya seeds can be maintained considerably at room temperature up to 8 mo. by storing the seed in sealed, preferably airtight, polythene bags or plastic bottles. Cold storage using polythene bags or plastic bottle is recommended [47].
\nThe storage conditions are very important. According to the classification of seed storage behavior, the papaya seed is classified as recalcitrant seed [48], others in intermediate seed [49]. In storage behavior ambient conditions, the papaya seeds survive for a short period of time [50] and are considered intermediate between recalcitrant and orthodox attribute and deteriorate rapidly at higher storage temperatures and relative humidity. Fresh seeds give higher germination rate and seedling vigor that will decline with increasing the storage time [51] and consider that the best conditions for papaya seed storage is when containing 6.0% moisture and stored at 0°C gave higher percentage of germination, lower dormancy, and seed death.
\nIn [18], the treatment that most affected the germination was the application of sodium hydroxide and the higher incidence of microorganisms, with high percentages of plants affected by fungi, which remained even below the germination approved for marketing, which requires, more than 60% [43].
\nIn [52], described some pre-sowing treatments with the finality of increase the germination, like preconditioned papaya seed at 24°C before transfer to 32°C, soaked in KNO3 for 30 min, soaking in gibberellic acid (GA3; 200 ppm) for 24 h resulted in highest germination percentage in soil compared to α-naphthalene acetic acid (NAA) and KNO3 treatment, and using a protocol of sterilization and germination of papaya seeds in response to light emitting diodes and got 100% of sterilization and 100% of germination.
\nThe efficiency of the different scarification methods has been demonstrated in several investigations to favor the germinative process. In [53] were evaluated several methods of scarification of pacain seeds (Chamaedorea sp.), where the germination results indicated that the highest percentage was 77.8% and that corresponded to cold water treatment for 15 days (physical scarification), followed by treatment with mechanical scarification by hammer blow with 77.17% compared to the absolute control that obtained a 5.67% of germination.
\nWith chemical scarification methods also obtained positive results [54], and verified germination percentages of 97 and 94%, respectively, in seeds of Centrosema macrocarpum for 10 min immersion.
\nThe treatments used release the aril at different time intervals and there are differences between them in relation to the mechanical damage and the quantity of abnormal seeds of the Maradol variety (Table 3). The best results were obtained when calcium hydroxide was applied at the 100 l−1 dose and with sodium hydroxide. Those compounds only needed an average time of 12 s to achieve an efficiency in the detachment of the aril, while with the application of the control dose, the worst results were obtained, with an average of 38 s that in some cases reached reaching more than 420 s to achieve the release of the aril, so that with this result, it is inferred that to work 25 kg of wet seeds, it would take approximately 38 min if treated with sodium hydroxide or calcium hydroxide in 100 l dose and approximately 106 min for the case fermented only with water.
\nCompound | \nDose | \nAril (s) | \nMD (%) | \nAP (%) | \n
---|---|---|---|---|
Aver. ± SD | \nAver. ± SD | \nAver. ± SD | \n||
Ca(OH)2 | \n60 g l−1 | \n21.2cd ± 1.3 | \n1.0a ± 0.9 | \n1.5ab ± 1.5 | \n
Ca(OH)2 | \n80 g l−1 | \n17.7cd ± 2.5 | \n1.0a ± 0.9 | \n0.7a ± 1.0 | \n
Ca(OH)2 | \n100 g l−1 | \n13.3ab ± 1.4 | \n0.5a ± 0.6 | \n0.4a ± 0.7 | \n
NaOH | \n25 g l−1 | \n12.0a ± 0.9 | \n0.5a ± 0.2 | \n3.3b ± 1.0 | \n
Fermentation \n | \n37.7d ± 6.3 | \n9.2b ± 1.2 | \n0.8a ± 0.9 | \n
Effect of calcium hydroxide and sodium hydroxide with different doses in the time (s) of detachment of the seed’s aril.
Different letters indicate significant differences to p ≤ 0.05 through the differences between the average of the ranges.
When carrying out an essay [55], with different methods to improve the germination of four forage shrubs legumes [Tagasaste (Cytisus proliferus) and three species of Teline (Genista)], obtained the most encouraging results for the elimination of the aril in the treatment with concentrated sulfuric acid for 30 min, demonstrating the effectiveness of chemical compounds in the scarification of seeds.
\nRefs. [7, 20] observed a negative effect on the papaya germination due to the presence in the aril or sarcotesta of inhibitory substances. It is also said that the marked decrease in germination, in the presence of sarcotesta is due to the low oxygenation of the seeds, which is why it is recommended to remove it. Likewise, in a study carried out in Honduras with seeds of the papaya, Maradol variety, higher percentages of germination were obtained with freshly harvested and oared seeds (83%), while fresh seeds with burned arils showed a lower percentage of germination, with 75% [56].
\nSimilar results presented by [18, 20] which treated seeds with very corrosive products not only the germination is affected, but that several plants emerged with problems fundamentally in the radicle and hypocotyl, assuming the influence of other factors such as fluctuation in the moisture of the seed in the time of conservation.
\nThe variables that were selected for the multiple correlation analyses (Table 4) showed that the release of aril seed correlated significantly with germination and vigor 360 days, positively and in a mean size. No correlations were found between this variable and the rest of the values of germination and vigor evaluated. Candiani et al. [57] concluded that the germination of Michelia champaca L. seeds is hindered probably due to the presence of inhibiting substances in the aril, is considered as endogenous causes of seed dormancy which include factors such as phytohormones, or by interference with water uptake [58]. Abscisic acid (ABA) is the key inhibitor of germination in Taxus yunnanensis seeds during wet sand storage [59].
\nCorrelations analysis | \n|||
---|---|---|---|
\n | Aril | \nMD | \nAdnor | \n
Ger 0 | \n0.13 ns | \n0.07 ns | \n−0.74* | \n
Ger120 | \n0.24 ns | \n0.15 ns | \n−0.71* | \n
Ger240 | \n0.25 ns | \n0.18 ns | \n−0.72* | \n
Ger360 | \n0.46* | \n0.34 ns | \n−0.70* | \n
Vigor | \n0.13 ns | \n0.08 ns | \n−0.73* | \n
Vigor120 | \n0.28 ns | \n0.19 ns | \n−0.70* | \n
Vigor240 | \n0.30 ns | \n0.22 ns | \n−0.69* | \n
Vigor360 | \n0.46* | \n0.34 ns | \n−0.70* | \n
Spearman correlations coefficient between variables.
Indicate significant differences to p ≤ 0.005.
Ger is germination, MD is mechanical damage, and adnor is adnormal plants.
The mechanical damage caused to the seeds during this process in this experiment did not correlate with the different levels of germination and vigor that were studied; however, the percentages of abnormal plants in the different time intervals evaluated showed a significant correlation, with medium to high values, but inverse and indicates that as the percentage of abnormal plants increases in a seed lot, the number of seeds germinates decreases and the vigor. Germination vigor is driven by the ability of the plant embryo, embedded within the seed, to resume its metabolic activity in a coordinated and sequential manner.
\nWas analyzed the vigor tests on lettuce (Lactuca sativa L.) seeds and their correlation with emergence [60], demonstrated that saturated salt accelerated aging and digital image analysis were the best laboratory tests for lettuce seed vigor evaluation, especially for seed lots to be used for plug seedling production. In some case, the use of seed vigor tests is used to predict field emergence in plants, like lucerne (Medicago sativa) [61].
\nIn studies [62] about studied the correlations of seed germination percent of two sweet corn hybrids (Zea mays L.) with field emergence and some measured traits related to yield, the results of correlation analysis indicated that there was a high positive correlation between seed germination ability and vigor with seedling field emergence and most of the measured traits, as the percent of the radicle emergence.
\nThe germination vigor depends on multiple biochemical and molecular variables. Their characterization is expected to deliver new markers of seed quality that can be used in breeding programs and/or in biotechnological approaches to improve crop yields [63].
\nCalcium hydroxide has great potential to be used as biocide in agriculture, because it has the advantage of not being phytotoxic, is economic and easy to use and is harmless to the environment and to humans. Decreased Ca levels in the nutrient medium reduced soybean leaf dry matter during seed fill, seed production, seed Ca concentration, and seed germination and increased the incidence of seedling disorders such as watery hypocotyl and epicotyl necrosis [64].
\nIn [65] performed standard germination tests, germination and growth rate, accelerated aging, electrical conductivity, respiration rate and ATP content, to evaluate the vigor of the seeds of Bromus biebersteinii Roem & Schult and determined that the correlations with the behavior in the field of that forage were accelerated aging, respiration rate, and ATP content. The cited authors conclude that a vigor test alone is not adequate to measure the quality of seed lots. A combination of tests which measure both physiological and biochemical aspects should be used.
\nSome researches [66] showed that seed size is an important factor for germination and seedling vigor, establishing that larger seeds produce more vigorous seedlings but with slower emergence. So it can be argued that by using the largest scarified seeds that we have, we can help considerably to decrease the percent of abnormal plants and at the same time increase the vigor and the percentage of germination, variables evaluated in the present investigation.
\nTo correlate characters related to seed germination, is important to investigate the effects of environmental factors prevailing during seed maturation under controlled conditions to understand exact reasons for unusual seed dormancy and germination requirements, for example, the germination of Citrullus colocynthis (Cucurbitaceae) is very sensitive to light and incubation temperature as well as to the environmental conditions associated with the time of seed maturation [67] and seed dormancy is a temporary failure of a viable seed to complete germination under normally favorable physical environmental conditions [33].
\nSeed germination tests assess the ability of the seed to produce a healthy plant when placed under favorable environmental conditions. Germination tests are conducted for a prescribed time period under laboratory conditions that assure optimum moisture, temperature, and light. Unfortunately, these conditions are seldom encountered in the field, and field emergence may be overestimated by standard germination tests. Seed lots that have low germination also are less vigorous due to seed deterioration. As seeds deteriorate, loss of vigor precedes loss of viability, so seeds with low germination usually will be less vigorous. Hence, in seed lots with poor germination, those seeds that do germinate often produce weaker seedlings with reduced yield potential. However, some species (such as many native grasses) have inherently low germination potential and cannot be assumed to have poor vigor due to low germination. Seed vigor usually cannot be assessed by the consumer. Germination is a good indicator of seed vigor [63, 68].
\nUsing efficient methods to scarify papaya seeds can increase the germination percentages of seeds, only if there is good control of environmental factors, because papaya seed germination is affected by light, temperature, oxygen, pH, and the moisture of the substrate [7].
\nIt is necessary to conduct researches about the biometric and morphological characteristics of fruit and seeds, aiming at the maximum germination capacity and seed vigor [69], because biometric studies of seeds and their phenotypical correlations allow the quantitative evaluation of a character’s relevance in relation to another [70]. It continues to investigate the correlations between the different indicators that can characterize the quality of the seeds. In adaptive correlations between seed size and germination time [71], present a model for the coevolution of seed size and germination time within a season when both affect the ability of the seedlings to compete for space and show that even in the absence of a morphological or physiological constraint between the two traits, a correlation between seed size and germination time is nevertheless likely to evolve.
\nSeed germination is a complex process and we need to understand the underlying molecular, hormonal, and mechanical aspects [72]. The environment during seed production has major impacts on the behavior of progeny seeds [73]. For that reason, the seed biology is considered the principal research topic for food security take into consideration the climate change [72].
\nNowadays, there are advances in the propagation of papaya by biotechnological methods. Efficient micropropagation of papaya has become crucial for the multiplication of specific sex types of papaya and in the application of genetic transformation technologies. Significant progress has been achieved using organogenesis and somatic embryogenesis as the shoot tip, axillary bud and single node culture, organogenesis, anther and ovule culture, and regeneration from protoplasts, callus induction and somatic embryogenesis and the mass propagation by ex vitro rooting and acclimatation [74].
\nIn natural conditions, the germination of papaya seeds has difficult by the presence of aril (sarcotesta) that become in a physical barrier which limits the diffusion of water and gases into the seeds and by the effect of phytohormones which preventing germination of seeds, causing dormancy, limiting the development of the embryos and causes a low and variable germination affecting the final percentage. This problem can be solved with the scarification of papaya seed. The results of this research showed that the dormancy by the presence of aril is produced in the papaya seed can be broken with the use of NaOH; but higher results were achieved with the use of calcium hydroxide, Ca(OH)2. The results suggested that chemical scarification with calcium hydroxide can improve germination percentage and vigor of the papaya seeds, take into account that the seed is considered the major essential input in crop production. If the seed quality parameters (vigor, germination rate) decrease, the yields are affected. The scarification of papaya seeds with the use of calcium hydroxide for its proven effect in this research on the benefit of papaya seeds and easy acquisition and will reduce the costs of seed. But a good germination of the papaya seedlings depends on the many environmental factors and excellent agronomy practice. Finally, the effectiveness of scarification methods could change among cultivars within the papaya specie.
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She performed research in perioperative autotransfusion and obtained the degree of PhD in 1993 publishing Peri-operative autotransfusion by means of a blood cell separator.\nBlood transfusion had her special interest being the president of the Haemovigilance Chamber TRIP and performing several tasks in local and national blood bank and anticoagulant-blood transfusion guidelines committees. Currently, she is working as an associate professor and up till recently was the dean at the Albert Schweitzer Hospital Dordrecht. She performed (inter)national tasks as vice-president of the Concilium Anaesthesia and related committees. \nShe performed research in several fields, with over 100 publications in (inter)national journals and numerous papers on scientific conferences. \nShe received several awards and is a member of Honour of the Dutch Society of Anaesthesia.",institutionString:null,institution:{name:"Albert Schweitzer Hospital",country:{name:"Gabon"}}},{id:"83089",title:"Prof.",name:"Aaron",middleName:null,surname:"Ojule",slug:"aaron-ojule",fullName:"Aaron Ojule",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Port Harcourt",country:{name:"Nigeria"}}},{id:"295748",title:"Mr.",name:"Abayomi",middleName:null,surname:"Modupe",slug:"abayomi-modupe",fullName:"Abayomi Modupe",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/no_image.jpg",biography:null,institutionString:null,institution:{name:"Landmark University",country:{name:"Nigeria"}}},{id:"94191",title:"Prof.",name:"Abbas",middleName:null,surname:"Moustafa",slug:"abbas-moustafa",fullName:"Abbas Moustafa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94191/images/96_n.jpg",biography:"Prof. Moustafa got his doctoral degree in earthquake engineering and structural safety from Indian Institute of Science in 2002. He is currently an associate professor at Department of Civil Engineering, Minia University, Egypt and the chairman of Department of Civil Engineering, High Institute of Engineering and Technology, Giza, Egypt. He is also a consultant engineer and head of structural group at Hamza Associates, Giza, Egypt. Dr. Moustafa was a senior research associate at Vanderbilt University and a JSPS fellow at Kyoto and Nagasaki Universities. He has more than 40 research papers published in international journals and conferences. He acts as an editorial board member and a reviewer for several regional and international journals. His research interest includes earthquake engineering, seismic design, nonlinear dynamics, random vibration, structural reliability, structural health monitoring and uncertainty modeling.",institutionString:null,institution:{name:"Minia University",country:{name:"Egypt"}}},{id:"84562",title:"Dr.",name:"Abbyssinia",middleName:null,surname:"Mushunje",slug:"abbyssinia-mushunje",fullName:"Abbyssinia Mushunje",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of Fort Hare",country:{name:"South Africa"}}},{id:"202206",title:"Associate Prof.",name:"Abd Elmoniem",middleName:"Ahmed",surname:"Elzain",slug:"abd-elmoniem-elzain",fullName:"Abd Elmoniem Elzain",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Kassala University",country:{name:"Sudan"}}},{id:"98127",title:"Dr.",name:"Abdallah",middleName:null,surname:"Handoura",slug:"abdallah-handoura",fullName:"Abdallah Handoura",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"École Supérieure des Télécommunications",country:{name:"Morocco"}}},{id:"91404",title:"Prof.",name:"Abdecharif",middleName:null,surname:"Boumaza",slug:"abdecharif-boumaza",fullName:"Abdecharif Boumaza",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Abbès Laghrour University of Khenchela",country:{name:"Algeria"}}},{id:"105795",title:"Prof.",name:"Abdel Ghani",middleName:null,surname:"Aissaoui",slug:"abdel-ghani-aissaoui",fullName:"Abdel Ghani Aissaoui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/105795/images/system/105795.jpeg",biography:"Abdel Ghani AISSAOUI is a Full Professor of electrical engineering at University of Bechar (ALGERIA). He was born in 1969 in Naama, Algeria. He received his BS degree in 1993, the MS degree in 1997, the PhD degree in 2007 from the Electrical Engineering Institute of Djilali Liabes University of Sidi Bel Abbes (ALGERIA). He is an active member of IRECOM (Interaction Réseaux Electriques - COnvertisseurs Machines) Laboratory and IEEE senior member. He is an editor member for many international journals (IJET, RSE, MER, IJECE, etc.), he serves as a reviewer in international journals (IJAC, ECPS, COMPEL, etc.). He serves as member in technical committee (TPC) and reviewer in international conferences (CHUSER 2011, SHUSER 2012, PECON 2012, SAI 2013, SCSE2013, SDM2014, SEB2014, PEMC2014, PEAM2014, SEB (2014, 2015), ICRERA (2015, 2016, 2017, 2018,-2019), etc.). His current research interest includes power electronics, control of electrical machines, artificial intelligence and Renewable energies.",institutionString:"University of Béchar",institution:{name:"University of Béchar",country:{name:"Algeria"}}},{id:"99749",title:"Dr.",name:"Abdel Hafid",middleName:null,surname:"Essadki",slug:"abdel-hafid-essadki",fullName:"Abdel Hafid Essadki",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"École Nationale Supérieure de Technologie",country:{name:"Algeria"}}},{id:"101208",title:"Prof.",name:"Abdel Karim",middleName:"Mohamad",surname:"El Hemaly",slug:"abdel-karim-el-hemaly",fullName:"Abdel Karim El Hemaly",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/101208/images/733_n.jpg",biography:"OBGYN.net Editorial Advisor Urogynecology.\nAbdel Karim M. A. El-Hemaly, MRCOG, FRCS � Egypt.\n \nAbdel Karim M. A. El-Hemaly\nProfessor OB/GYN & Urogynecology\nFaculty of medicine, Al-Azhar University \nPersonal Information: \nMarried with two children\nWife: Professor Laila A. Moussa MD.\nSons: Mohamad A. M. El-Hemaly Jr. MD. Died March 25-2007\nMostafa A. M. El-Hemaly, Computer Scientist working at Microsoft Seatle, USA. \nQualifications: \n1.\tM.B.-Bch Cairo Univ. June 1963. \n2.\tDiploma Ob./Gyn. Cairo Univ. April 1966. \n3.\tDiploma Surgery Cairo Univ. Oct. 1966. \n4.\tMRCOG London Feb. 1975. \n5.\tF.R.C.S. Glasgow June 1976. \n6.\tPopulation Study Johns Hopkins 1981. \n7.\tGyn. Oncology Johns Hopkins 1983. \n8.\tAdvanced Laparoscopic Surgery, with Prof. Paulson, Alexandria, Virginia USA 1993. \nSocieties & Associations: \n1.\t Member of the Royal College of Ob./Gyn. London. \n2.\tFellow of the Royal College of Surgeons Glasgow UK. \n3.\tMember of the advisory board on urogyn. FIGO. \n4.\tMember of the New York Academy of Sciences. \n5.\tMember of the American Association for the Advancement of Science. \n6.\tFeatured in �Who is Who in the World� from the 16th edition to the 20th edition. \n7.\tFeatured in �Who is Who in Science and Engineering� in the 7th edition. \n8.\tMember of the Egyptian Fertility & Sterility Society. \n9.\tMember of the Egyptian Society of Ob./Gyn. \n10.\tMember of the Egyptian Society of Urogyn. \n\nScientific Publications & Communications:\n1- Abdel Karim M. El Hemaly*, Ibrahim M. Kandil, Asim Kurjak, Ahmad G. Serour, Laila A. S. Mousa, Amr M. Zaied, Khalid Z. El Sheikha. \nImaging the Internal Urethral Sphincter and the Vagina in Normal Women and Women Suffering from Stress Urinary Incontinence and Vaginal Prolapse. Gynaecologia Et Perinatologia, Vol18, No 4; 169-286 October-December 2009.\n2- Abdel Karim M. El Hemaly*, Laila A. S. Mousa Ibrahim M. Kandil, Fatma S. El Sokkary, Ahmad G. Serour, Hossam Hussein.\nFecal Incontinence, A Novel Concept: The Role of the internal Anal sphincter (IAS) in defecation and fecal incontinence. Gynaecologia Et Perinatologia, Vol19, No 2; 79-85 April -June 2010.\n3- Abdel Karim M. El Hemaly*, Laila A. S. Mousa Ibrahim M. Kandil, Fatma S. El Sokkary, Ahmad G. Serour, Hossam Hussein.\nSurgical Treatment of Stress Urinary Incontinence, Fecal Incontinence and Vaginal Prolapse By A Novel Operation \n"Urethro-Ano-Vaginoplasty"\n Gynaecologia Et Perinatologia, Vol19, No 3; 129-188 July-September 2010.\n4- Abdel Karim M. El Hemaly*, Ibrahim M. Kandil, Laila A. S. Mousa and Mohamad A.K.M.El Hemaly.\nUrethro-vaginoplasty, an innovated operation for the treatment of: Stress Urinary Incontinence (SUI), Detursor Overactivity (DO), Mixed Urinary Incontinence and Anterior Vaginal Wall Descent. \nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/ urethro-vaginoplasty_01\n\n5- Abdel Karim M. El Hemaly, Ibrahim M Kandil, Mohamed M. Radwan.\n Urethro-raphy a new technique for surgical management of Stress Urinary Incontinence.\nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/\nnew-tech-urethro\n\n6- Abdel Karim M. El Hemaly, Ibrahim M Kandil, Mohamad A. Rizk, Nabil Abdel Maksoud H., Mohamad M. Radwan, Khalid Z. El Shieka, Mohamad A. K. M. El Hemaly, and Ahmad T. El Saban.\nUrethro-raphy The New Operation for the treatment of stress urinary incontinence, SUI, detrusor instability, DI, and mixed-type of urinary incontinence; short and long term results. \nhttp://www.obgyn.net/urogyn/urogyn.asp?page=urogyn/articles/\nurethroraphy-09280\n\n7-Abdel Karim M. El Hemaly, Ibrahim M Kandil, and Bahaa E. El Mohamady. Menopause, and Voiding troubles. \nhttp://www.obgyn.net/displayppt.asp?page=/English/pubs/features/presentations/El-Hemaly03/el-hemaly03-ss\n\n8-El Hemaly AKMA, Mousa L.A. Micturition and Urinary\tContinence. Int J Gynecol Obstet 1996; 42: 291-2. \n\n9-Abdel Karim M. El Hemaly.\n Urinary incontinence in gynecology, a review article.\nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/abs-urinary_incotinence_gyn_ehemaly \n\n10-El Hemaly AKMA. Nocturnal Enuresis: Pathogenesis and Treatment. \nInt Urogynecol J Pelvic Floor Dysfunct 1998;9: 129-31.\n \n11-El Hemaly AKMA, Mousa L.A.E. Stress Urinary Incontinence, a New Concept. Eur J Obstet Gynecol Reprod Biol 1996; 68: 129-35. \n\n12- El Hemaly AKMA, Kandil I. M. Stress Urinary Incontinence SUI facts and fiction. Is SUI a puzzle?! http://www.obgyn.net/displayppt.asp?page=/English/pubs/features/presentations/El-Hemaly/el-hemaly-ss\n\n13-Abdel Karim El Hemaly, Nabil Abdel Maksoud, Laila A. Mousa, Ibrahim M. Kandil, Asem Anwar, M.A.K El Hemaly and Bahaa E. El Mohamady. \nEvidence based Facts on the Pathogenesis and Management of SUI. http://www.obgyn.net/displayppt.asp?page=/English/pubs/features/presentations/El-Hemaly02/el-hemaly02-ss\n\n14- Abdel Karim M. El Hemaly*, Ibrahim M. Kandil, Mohamad A. Rizk and Mohamad A.K.M.El Hemaly.\n Urethro-plasty, a Novel Operation based on a New Concept, for the Treatment of Stress Urinary Incontinence, S.U.I., Detrusor Instability, D.I., and Mixed-type of Urinary Incontinence.\nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/urethro-plasty_01\n\n15-Ibrahim M. Kandil, Abdel Karim M. El Hemaly, Mohamad M. Radwan: Ultrasonic Assessment of the Internal Urethral Sphincter in Stress Urinary Incontinence. The Internet Journal of Gynecology and Obstetrics. 2003. Volume 2 Number 1. \n\n\n16-Abdel Karim M. El Hemaly. Nocturnal Enureses: A Novel Concept on its pathogenesis and Treatment.\nhttp://www.obgyn.net/urogynecolgy/?page=articles/nocturnal_enuresis\n\n17- Abdel Karim M. El Hemaly. Nocturnal Enureses: An Update on the pathogenesis and Treatment.\nhttp://www.obgyn.net/urogynecology/?page=/ENHLIDH/PUBD/FEATURES/\nPresentations/ Nocturnal_Enuresis/nocturnal_enuresis\n\n18-Maternal Mortality in Egypt, a cry for help and attention. The Second International Conference of the African Society of Organization & Gestosis, 1998, 3rd Annual International Conference of Ob/Gyn Department � Sohag Faculty of Medicine University. Feb. 11-13. Luxor, Egypt. \n19-Postmenopausal Osteprosis. The 2nd annual conference of Health Insurance Organization on Family Planning and its role in primary health care. Zagaziz, Egypt, February 26-27, 1997, Center of Complementary Services for Maternity and childhood care. \n20-Laparoscopic Assisted vaginal hysterectomy. 10th International Annual Congress Modern Trends in Reproductive Techniques 23-24 March 1995. Alexandria, Egypt. \n21-Immunological Studies in Pre-eclamptic Toxaemia. Proceedings of 10th Annual Ain Shams Medical Congress. Cairo, Egypt, March 6-10, 1987. \n22-Socio-demographic factorse affecting acceptability of the long-acting contraceptive injections in a rural Egyptian community. Journal of Biosocial Science 29:305, 1987. \n23-Plasma fibronectin levels hypertension during pregnancy. The Journal of the Egypt. Soc. of Ob./Gyn. 13:1, 17-21, Jan. 1987. \n24-Effect of smoking on pregnancy. Journal of Egypt. Soc. of Ob./Gyn. 12:3, 111-121, Sept 1986. \n25-Socio-demographic aspects of nausea and vomiting in early pregnancy. Journal of the Egypt. Soc. of Ob./Gyn. 12:3, 35-42, Sept. 1986. \n26-Effect of intrapartum oxygen inhalation on maternofetal blood gases and pH. Journal of the Egypt. Soc. of Ob./Gyn. 12:3, 57-64, Sept. 1986. \n27-The effect of severe pre-eclampsia on serum transaminases. The Egypt. J. Med. Sci. 7(2): 479-485, 1986. \n28-A study of placental immunoreceptors in pre-eclampsia. The Egypt. J. Med. Sci. 7(2): 211-216, 1986. \n29-Serum human placental lactogen (hpl) in normal, toxaemic and diabetic pregnant women, during pregnancy and its relation to the outcome of pregnancy. Journal of the Egypt. Soc. of Ob./Gyn. 12:2, 11-23, May 1986. \n30-Pregnancy specific B1 Glycoprotein and free estriol in the serum of normal, toxaemic and diabetic pregnant women during pregnancy and after delivery. Journal of the Egypt. Soc. of Ob./Gyn. 12:1, 63-70, Jan. 1986. Also was accepted and presented at Xith World Congress of Gynecology and Obstetrics, Berlin (West), September 15-20, 1985. \n31-Pregnancy and labor in women over the age of forty years. Accepted and presented at Al-Azhar International Medical Conference, Cairo 28-31 Dec. 1985. \n32-Effect of Copper T intra-uterine device on cervico-vaginal flora. Int. J. Gynaecol. Obstet. 23:2, 153-156, April 1985. \n33-Factors affecting the occurrence of post-Caesarean section febrile morbidity. Population Sciences, 6, 139-149, 1985. \n34-Pre-eclamptic toxaemia and its relation to H.L.A. system. Population Sciences, 6, 131-139, 1985. \n35-The menstrual pattern and occurrence of pregnancy one year after discontinuation of Depo-medroxy progesterone acetate as a postpartum contraceptive. Population Sciences, 6, 105-111, 1985. \n36-The menstrual pattern and side effects of Depo-medroxy progesterone acetate as postpartum contraceptive. Population Sciences, 6, 97-105, 1985. \n37-Actinomyces in the vaginas of women with and without intrauterine contraceptive devices. Population Sciences, 6, 77-85, 1985. \n38-Comparative efficacy of ibuprofen and etamsylate in the treatment of I.U.D. menorrhagia. Population Sciences, 6, 63-77, 1985. \n39-Changes in cervical mucus copper and zinc in women using I.U.D.�s. Population Sciences, 6, 35-41, 1985. \n40-Histochemical study of the endometrium of infertile women. Egypt. J. Histol. 8(1) 63-66, 1985. \n41-Genital flora in pre- and post-menopausal women. Egypt. J. Med. Sci. 4(2), 165-172, 1983. \n42-Evaluation of the vaginal rugae and thickness in 8 different groups. Journal of the Egypt. Soc. of Ob./Gyn. 9:2, 101-114, May 1983. \n43-The effect of menopausal status and conjugated oestrogen therapy on serum cholesterol, triglycerides and electrophoretic lipoprotein patterns. Al-Azhar Medical Journal, 12:2, 113-119, April 1983. \n44-Laparoscopic ventrosuspension: A New Technique. Int. J. Gynaecol. Obstet., 20, 129-31, 1982. \n45-The laparoscope: A useful diagnostic tool in general surgery. Al-Azhar Medical Journal, 11:4, 397-401, Oct. 1982. \n46-The value of the laparoscope in the diagnosis of polycystic ovary. Al-Azhar Medical Journal, 11:2, 153-159, April 1982. \n47-An anaesthetic approach to the management of eclampsia. Ain Shams Medical Journal, accepted for publication 1981. \n48-Laparoscopy on patients with previous lower abdominal surgery. Fertility management edited by E. Osman and M. Wahba 1981. \n49-Heart diseases with pregnancy. Population Sciences, 11, 121-130, 1981. \n50-A study of the biosocial factors affecting perinatal mortality in an Egyptian maternity hospital. Population Sciences, 6, 71-90, 1981. \n51-Pregnancy Wastage. Journal of the Egypt. Soc. of Ob./Gyn. 11:3, 57-67, Sept. 1980. \n52-Analysis of maternal deaths in Egyptian maternity hospitals. Population Sciences, 1, 59-65, 1979. \nArticles published on OBGYN.net: \n1- Abdel Karim M. El Hemaly*, Ibrahim M. Kandil, Laila A. S. Mousa and Mohamad A.K.M.El Hemaly.\nUrethro-vaginoplasty, an innovated operation for the treatment of: Stress Urinary Incontinence (SUI), Detursor Overactivity (DO), Mixed Urinary Incontinence and Anterior Vaginal Wall Descent. \nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/ urethro-vaginoplasty_01\n\n2- Abdel Karim M. El Hemaly, Ibrahim M Kandil, Mohamed M. Radwan.\n Urethro-raphy a new technique for surgical management of Stress Urinary Incontinence.\nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/\nnew-tech-urethro\n\n3- Abdel Karim M. El Hemaly, Ibrahim M Kandil, Mohamad A. Rizk, Nabil Abdel Maksoud H., Mohamad M. Radwan, Khalid Z. El Shieka, Mohamad A. K. M. El Hemaly, and Ahmad T. El Saban.\nUrethro-raphy The New Operation for the treatment of stress urinary incontinence, SUI, detrusor instability, DI, and mixed-type of urinary incontinence; short and long term results. \nhttp://www.obgyn.net/urogyn/urogyn.asp?page=urogyn/articles/\nurethroraphy-09280\n\n4-Abdel Karim M. El Hemaly, Ibrahim M Kandil, and Bahaa E. El Mohamady. Menopause, and Voiding troubles. \nhttp://www.obgyn.net/displayppt.asp?page=/English/pubs/features/presentations/El-Hemaly03/el-hemaly03-ss\n\n5-El Hemaly AKMA, Mousa L.A. Micturition and Urinary\tContinence. Int J Gynecol Obstet 1996; 42: 291-2. \n\n6-Abdel Karim M. El Hemaly.\n Urinary incontinence in gynecology, a review article.\nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/abs-urinary_incotinence_gyn_ehemaly \n\n7-El Hemaly AKMA. Nocturnal Enuresis: Pathogenesis and Treatment. \nInt Urogynecol J Pelvic Floor Dysfunct 1998;9: 129-31.\n \n8-El Hemaly AKMA, Mousa L.A.E. Stress Urinary Incontinence, a New Concept. Eur J Obstet Gynecol Reprod Biol 1996; 68: 129-35. \n\n9- El Hemaly AKMA, Kandil I. M. Stress Urinary Incontinence SUI facts and fiction. Is SUI a puzzle?! http://www.obgyn.net/displayppt.asp?page=/English/pubs/features/presentations/El-Hemaly/el-hemaly-ss\n\n10-Abdel Karim El Hemaly, Nabil Abdel Maksoud, Laila A. Mousa, Ibrahim M. Kandil, Asem Anwar, M.A.K El Hemaly and Bahaa E. El Mohamady. \nEvidence based Facts on the Pathogenesis and Management of SUI. http://www.obgyn.net/displayppt.asp?page=/English/pubs/features/presentations/El-Hemaly02/el-hemaly02-ss\n\n11- Abdel Karim M. El Hemaly*, Ibrahim M. Kandil, Mohamad A. Rizk and Mohamad A.K.M.El Hemaly.\n Urethro-plasty, a Novel Operation based on a New Concept, for the Treatment of Stress Urinary Incontinence, S.U.I., Detrusor Instability, D.I., and Mixed-type of Urinary Incontinence.\nhttp://www.obgyn.net/urogyn/urogyn.asp?page=/urogyn/articles/urethro-plasty_01\n\n12-Ibrahim M. Kandil, Abdel Karim M. El Hemaly, Mohamad M. Radwan: Ultrasonic Assessment of the Internal Urethral Sphincter in Stress Urinary Incontinence. The Internet Journal of Gynecology and Obstetrics. 2003. Volume 2 Number 1. \n\n13-Abdel Karim M. El Hemaly. Nocturnal Enureses: A Novel Concept on its pathogenesis and Treatment.\nhttp://www.obgyn.net/urogynecolgy/?page=articles/nocturnal_enuresis\n\n14- Abdel Karim M. El Hemaly. 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