Most commonly used for the treatment of Parkinson’s disease (PD), the deep brain stimulation (DBS) is a new neurosurgical method whose other applications are still under development. Neuroimaging has a variety of main roles in DBS including evaluating the final electrode contact position, localizing the target nucleus, and detecting complications. Despite being a neurosurgical method, successful DBS intervention is highly dependent on an appropriate neuroimaging technique. For achieving satisfying clinical results, DBS needs the presence of neuroradiologists. In this chapter, we have reviewed the role of neuroimaging in all stages of deep brain stimulation as well as the underlying mechanism in this domain.