Range of exons for different methods
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Working as a clinical research fellow in Warwick Medical School he obtained the MPhil form University of Warwick and was elected for a research fellowship to University of California Los Angeles (UCLA). \r\nHis research interests include blood flow to the hip, failure of hip resurfacing, designing new hip prosthesis, and surgical approaches to the hip. \r\nHe has over 30 international publications and presentations and several book chapter. \r\nHe also works as a reviewer for international orthopedic journals and has reviewed over 35 papers and is a member of the editorial board of Sri Lanka Journal of Surgery.",institutionString:"University of Warwick Science Park",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"3",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"University of Warwick Science Park",institutionURL:null,country:{name:"United Kingdom"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:[{id:"73224",title:"Management of Early Osteoarthritis",slug:"management-of-early-osteoarthritis",totalDownloads:113,totalCrossrefCites:0,authors:[null]},{id:"71336",title:"Non-Surgical Regional Therapy for Osteoarthritis: An Update and Review of the Literature",slug:"non-surgical-regional-therapy-for-osteoarthritis-an-update-and-review-of-the-literature",totalDownloads:182,totalCrossrefCites:0,authors:[{id:"77195",title:"Dr.",name:"Charles",surname:"Mackworth-Young",slug:"charles-mackworth-young",fullName:"Charles Mackworth-Young"}]},{id:"72715",title:"Simultaneous Bilateral Joint Arthroplasties in Treatment of Osteoarthritis",slug:"simultaneous-bilateral-joint-arthroplasties-in-treatment-of-osteoarthritis",totalDownloads:121,totalCrossrefCites:1,authors:[null]},{id:"75062",title:"Bone Cancer Pain, Mechanism and Treatment",slug:"bone-cancer-pain-mechanism-and-treatment",totalDownloads:37,totalCrossrefCites:0,authors:[null]},{id:"75735",title:"Osteosarcoma",slug:"osteosarcoma",totalDownloads:23,totalCrossrefCites:0,authors:[null]}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"247865",firstName:"Jasna",lastName:"Bozic",middleName:null,title:"Ms.",imageUrl:"https://mts.intechopen.com/storage/users/247865/images/7225_n.jpg",email:"jasna.b@intechopen.com",biography:"As an Author Service Manager, my responsibilities include monitoring and facilitating all publishing activities for authors and editors. 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The DNA is isolated from blood through a series of different procedures including heat shock, thermal change and applications of different chemicals etc. DNA sequence contains chromosomes which further contains genes over them. The genes have regions which could translate to protein and the regions which don’t perform any contribution in protein production. Both kinds of regions are made-up of nucleotides characterized as Adenine, Thymine, Cytosine and Guanine. The order of these nucleotides determines the traits, habits and livings of all species. Since with the exponential growth of biological data, there is an enormous amount of such data that needs to be translated to protein. A successful translation would result in knowing important information about species.
Comparative analysis of computational techniques employed over genetic datasets has given very interesting results. We are able to identify species from each other on the behalf of DNA properties. A true correct conversion takes to fruitful results. Literature has shown that direct comparative analysis is not as useful as approximate estimation. So far, there is no compact solution available that could outperform for a robust translation from DNA to RNA.
It is a common phenomenon that nucleotide sequences in DNA perform a period three property [3, 11] due to codon composition and structure in the strand. This fundamental characteristic can be exploited to predict the codon regions that help in determination of RNA sequences in DNA. This finding is of immense importance as cell growth and function is determined by the type of protein the cell produces and helps in drug design and revealing genetic disorders as a result of mutation in structure of nucleotide bases (order in which they appear over chain). Many approaches have been proposed in literature that addresses this open optimization problem in computational biology.
Discrete Fourier Transforms [6, 7, and 8] normally result in spectral leakage that doesn’t preview the optimal power spectral density estimation. On the other hand, the Short Time Fourier Transforms [2, 4] minimize the leakage but are considered useful when we desire to have the frequency contents with location information. It can plot the components for time, amplitude and frequency of a genetic signal.
Digital Filters [5, 7, and 13] present the spectral contents of signal around the periodicity property of coding regions but don’t specify the frequency time relationship with amplitude.
Dosay-Akbulut [14] emphasized the classification of introns in two groups based on RNA secondary structure and self splicing ability in variant species using PCR.
A. Parent et al., [15] describe the importance of coordination between transcription and RNA processing that carboxy-terminal domain of RNA polymerase II acts as a common link in both.
Al Wadi et al. [16] used wavelet transforms for forecasting volatility in experimental results. M. Hashemi et al. [17] provided Identification of
A. Ali et al. [18] have presented a Histopathological Study for development of a model for Tumor Lung Cancer Assessing Anti-neoplastic Effect of PMF in Rodents.
J. Singh et al. [19] proposed a technique for Prediction of in vitro Drug Release Mechanisms from Extended Release Matrix Tablets.
The proposed approach consists of a series of components that analyze the DNA signal and enhances the prediction accuracy of genic regions over DNA sequence. The major steps of proposed approach are,
Conversion of target DNA stretch to a digital pattern employing an indicator sequence
Decomposition of signal using wavelet transforms
Calculations of approximate coefficients of signal at level three
Calculations of detail coefficients of signal
Density estimation of signal
Signal analysis for denosing
Depiction of original and synthesized signal at level three
Histogram estimations of signal
Signal extension to a desired length
Shannon entropy calculation of signal
Magnitude and power estimation of signal
Calculation of discrimination measure for PSD analysis
Exon and intron boundaries’ estimation
As an elaboration, the DNA sequence is passed through a filter that transforms it into a digital pattern. This phase is accomplished employing an indicator sequence with the following weights for nucleotides,
The corresponding transform becomes
Indicator sequence
The signal is decomposed employing the wavelet transforms of order three at level three
3rd order wavelet decomposition
The wavelet decomposition passes the signal into a series of low and high pass filters that decompose and synthesize the signal for reducing flicker noise (pink noise).
The signal is then convoluted with a window function (Kaiser Window) defined below,
Kaiser window of length 351 bp
Each section of the signal is traversed for calculation of absolute and power values. Each segment is plotted over the power spectral graph keeping the period three property maintained at each step. The exon boundaries appear as sharp peaks. The final discrimination measure depicts the degree of relevance in exon and introns.
A specimen gene pattern S.cerevisiae chromosome III (AF099922) has been taken for experiments over proposed approach. The gene is passed through the series of steps defined,
At processing stage, the dataset is passed through two kinds of filters. First filter refines the data and outputs a data file that purely contains nucleotide characters. Second filter operates on output file obtained from first filter application and generates a file that contains numeric data. This data is fed into central engine for further processing.
Figure 1 Shows dataset that contains nucleotide characters and some other characters. This is first necessary step because this input when fed into our engine will badly degrade the performance and brings false results.
Preprocessed dataset
Figure 2 represents a data glimpse that contains pure nucleotide characters.
Refined dataset
The EIIP indicator sequence transforms the nucleotides in numeric values as per its definition. A part of signal is described in Figure 3 below using EIIP indicator sequence as,
Numeric translation of gene F56F11.5 (AF099922)
The binary indicator sequence is formed by replacing the individual nucleotides with values either 0 or 1. 1 stands for presence and 0 for absence of a particular nucleotide in specified location in DNA signal,
Figure 4 describes the glimpse of binary indicator sequence which is the one of four parts of translation of gene file. Only 1\'s and 0\'s are visible in this sequence.
Binary indicator sequence
The complex indicator sequence is defined by replacing the nucleotide with 1, -1, iota and -iota values.
Figure 5 shows a portion of gene AF099922 after application of complex indicator sequence.
Complex indicator sequence applied to gene
The complex indicator sequence transforms the sequence into four digital patterns with associated weights. It is worth mentioning that this indicator sequence provided close range estimation for nucleotides in the literature.
This signal is then passed through the steps of windowed STFT for exonic prediction spectral analysis. This helps to extend the length of the signal to a target length so that perfect analysis could be performed over the signal.
Figure 6 shows that signal has been extended to a desired length. The length of signal was 8000 patterns. The convolution method suggests that to perform a better approximation, the signal should be extended to 8192 patterns. The signal should be mapped employing Kaiser Window of length 351 base pairs. The previous power of two shows a numerical value 4096 which truncates the signal from its original length. Truncation phenomenon can degrade the results and may bring faulty approximation that would lead to differ from the standard range of exons.
Figure 7 depicts the wavelet sketch for db3 wavelet. Scaling and wavelet functions have been described. Decomposition of low pass filter and high pass filters have been identified, similarly signal synthesis for low and high pass filters have been shown. This sketch demonstrates that signal should be passed through these defined filters to further analyze it for denoising and enhancement. The upward and downward curves self explains the convolution of signal with the window function at desired location of nucleotides.
Signal extension to desired length
Wavelet of db3 sketch
Figure 8 shows a wavelet tree for Shannon entropy of signal. There is a tree structure for the nodes depicting different position factors. Colored coefficients for terminal nodes can be observed. The first rectangle at the right top shows the analyzed signal at different nucleotides places (diffusion of bases at DNA strand). Calculation of Shannon entropy would assist in further identification of boundary values for individual nucleotides at power spectral density estimation graphs.
The digital signal passes through refinement stages. First, the sequence was obtained as a raw data which was purified to access only nucleotides bases without degrading factor. This is termed as an important process because any kind of unwanted characters may lead to different set of nucleotides values that would be away from actual results.
The digital signal under discussions contains 8000 base pairs. The same dataset was used extensively in literature by other researchers and it is being used as a bench mark. The spectral estimation graph reveals that it contains five exonic regions at different nucleotides ranges. Identification of these ranges close to standard range demands to denoise the signal and selection of an appropriate window function that could be used for perfect convolution. The standard convolution requires to multiply the signal with a portion of window function, this is the reason that signal was extended to a power of two to make it to desired length. Each frame of the signal is calculated numerically equal sized so that power spectral graph is uniform in all characteristics.
For discrete wavelet transforms of order three, the signal is decomposed and synthesized. These db3 performs the quick vanishing of coefficients for approximate and detail patterns.
Figure 9 shows a glimpse of original signal. There are 8000 base pairs shown in the form of a digital pattern. Cumulative histogram of signal shows different range of weight values assigned to nucleotides base pairs. It can be seen that nucleotides with numerals higher than 0.25 have high frequency while those between 0.1 and less than 0.25 have lower frequency. The individual histogram also shows three separate characterizations of nucleotide weight values. The standard deviation has been found to be 0.09037, median of absolute deviation is 0.11 while mean absolute deviation is 0.07843. The maximum range is 0.375 while minimum range is 0.125 and the average range is depicted as 0.25.
Wavelet tree for Shannon entropy
Original signal at level 3
The histogram calculates the frequency of nucleotides bases in the signal. Since the signal was mapped with an enhanced indicator sequence which assigns perfect weights to nucleotides bases, histogram of such a signal is uniform. It is observed that almost half of the signal is diffused in first band and half in the second band. First half band shows the smaller histogram values (frequency components) while second half band depicts some larger histogram values.
The individual histogram components dependant over the individual nucleotide bases, for instance, the numeric value of Adenine is 0.260, which is plotted against the other numeric values for bases in individual histogram. Depending over the weights assigned to Thymine and cytosine, the histogram shape may change.
It is also important to note that histogram of frequency components present the redundancy of bases in the digital pattern. This repetition depends over the order of nucleotides in DNA sequence which defines the habits, traits and other characteristics of species.
Figure 10 shows the synthesized signal at level three. Like the original signal, the synthesized signal owns the same histogram characteristics. There are 8000 base pairs shown in the form of a digital pattern. Cumulative histogram of signal shows different range of weight values assigned to nucleotides base pairs. It can be seen that nucleotides with numerals higher than 0.25 have high frequency while those between 0.1 and less than 0.25 have lower frequency. The individual histogram also shows three separate characterizations of nucleotide weight values. The standard deviation has been found to be 0.09037, median of absolute deviation is 0.11 while mean absolute deviation is 0.07843. The maximum range is 0.375 while minimum range is 0.125 and the average range is depicted as 0.25.
Synthesized signal at level 3
The synthesized signal shows the same histogram even after its decomposition. The synthesized signal is perfectly reconstructed by employing discrete wavelet transforms. The approximate and detail coefficients of signal are obtained in passing through a series of filters. These digital filters have been defined and constructed using Matlab. The decomposed signal is addition of approximate and detail coefficients at level three along with detail coefficients at level two and level one.
As for as, we decompose the signal, the components are loosely packed.
Figure 11 depicts the signal decomposition into approximate and detail coefficients. Symbol s represents the original signal. Approximate and detail coefficient at level three show the reduced complexity in the signal.
Signal decomposition
Figure 12 presents the histogram for approximate and detail coefficients. At level one, the concentration of components is less than other levels. Level two shows that signal components are more concentrated. At level three, the signal components are more closely packed. Likewise, the histogram for approximate coefficients presents the same phenomenon. At level one, the concentration of components is less than other levels. Level two shows that signal components are more concentrated. At level three, the signal components are more closely packed. It can be observed that original signal and synthesized signal contain the same number of components. The concentrations of signal components are uniform over these histograms’ plots, which depict the perfect reconstruction of digital signal.
Histogram of signal
Figure 13 shows the density estimation of approximate and detail coefficients. The density estimate of original signal shows the numerals for nucleotides present in the signal in digital format as a general. The approximate coefficients at level three presents a sharp peak at some 0.25 points. The signal remains uniform through the course except at another peak value ranging from 0.37 to the end of the signal. The density estimation for detail coefficients at level one shows the same sharp peak around 0.27 points. The same peak can be observed around 0.40 at level two. At level three, the phenomenon is same but the signal components are loosely packed than level two. At granular level, the components are more packed at level one than other levels.
Density estimation of signal
Figure 14 shows the resultant denoised signal. It is obvious that preview of detail coefficients at level three shows the loosely packed signal components. The original signal is represented in red color. The threshold coefficients are shown in vertical bars for all nucleotide range (8000 base pairs). The coefficients at detail level depicts a hierarchy of packed, loosely packed and more loosely packed components, which shows a gradual improvement in the signal for denosing.
Denoised signal
Figure 15 shows the approximate coefficients at level three. A sharp gradual change can be observed in a commutative histogram. The peaks are more pronounced at from point one to onwards. In another histogram, the peaks are not much visible around first 0.6 points, there is a sharp gradual increment in the bars reaching the maximum of 0.07 points then a gradual decrement is observed leading it to point one. The peaks are less pronounced after this point. The coefficients of approximation at level three show the signal as loosely packed components.
It can be observed that detail coefficient at level one are packed showing more concentration of nucleotides while detail coefficients at level two are loosely packed. The coefficients at level three are more significant than other levels, which represents that the signal is filtered for refinement. The signal was passed through a series of filters for the wavelet db3 which denoised the signal as a result of reconstruction of signal.
Approximation coefficients at level 3
Figure 16 shows the detail coefficients at level three. A sharp gradual change can be observed in a commutative histogram. The peaks are more pronounced at from point one to onwards. In another histogram, the peaks are not much visible around first 0.6 points, there is a sharp gradual increment in the bars reaching the maximum of 0.07 points then a gradual decrement is observed leading it to point one. The peaks are less pronounced after this point. The coefficients of detail at level three show the signal as loosely packed components.
Coefficients of detail at level 3
It can be observed that detail coefficient at level one are packed showing more concentration of nucleotides while detail coefficients at level two are loosely packed. The coefficients at level three are more significant than other levels, which represents that the signal is filtered for refinement. The signal was passed through a series of filters for the wavelet db3 which denoised the signal as a result of reconstruction of signal.
Table 1 presents the nucleotide range for exons. Clear differences can be observed as a comparative analysis of various approaches. Binary and EIIP methods show a wide range difference compared with the standard range. Complex method results are better than the first two approaches. Digital filters behave accordingly. The proposed approach has more significant results than other prevailing approaches.
\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t\t\n\t\t\t\t | \n\t\t
Binary Method | \n\t\t\t656-1206 | \n\t\t\t2406-3106 | \n\t\t\t3806-4406 | \n\t\t\t5306-5806 | \n\t\t\t7106-7706 | \n\t\t
EIIP Method | \n\t\t\t706-1206 | \n\t\t\t2206-2906 | \n\t\t\t3906-4406 | \n\t\t\t5206-5806 | \n\t\t\t7206-7706 | \n\t\t
Complex Method | \n\t\t\t750-1100 | \n\t\t\t2600-2906 | \n\t\t\t3600-4406 | \n\t\t\t5206-5706 | \n\t\t\t7106-7600 | \n\t\t
Filter 1 (Anti-notch) | \n\t\t\t656-1206 | \n\t\t\t2450-3106 | \n\t\t\t3806-4450 | \n\t\t\t5306-5850 | \n\t\t\t7106-7750 | \n\t\t
Filter 2 (Multistage) | \n\t\t\t706-1250 | \n\t\t\t2206-2950 | \n\t\t\t3906-4450 | \n\t\t\t5206-5850 | \n\t\t\t7206-7706 | \n\t\t
proposed Method | \n\t\t\t750-1050 | \n\t\t\t2450-2906 | \n\t\t\t3950-4380 | \n\t\t\t5206-5600 | \n\t\t\t7220-7680 | \n\t\t
NCBI Range | \n\t\t\t928-1039 | \n\t\t\t2528-2857 | \n\t\t\t4114-4377 | \n\t\t\t5465-5644 | \n\t\t\t7255-7605 | \n\t\t
Range of exons for different methods
Bioinformatics is a very rapidly emerging field of research. The genome sequence analysis is an interesting and challenging task that needs great attention. The analysis brings very promising relevance between species. The proposed approach provides a way to better identify the genetic regions in mixture of exon-intron noise. The focus directed to minimize the leakage of frequency contents by adoption of an optimal indicator sequence. We also reduced the signal noise by using Kaiser Window function with length 351 base pairs. The spectral density estimation was enhanced with application of wavelet transforms. The proposed dimensions reduced the noise and increased the sharp peaks of exons in density graphs. We have observed significant improvement in results as a comparative analysis between existing techniques and compared the results with strands NCBI range.
In 1948, the World Health Organization (WHO) defined health as ‘a state of complete physical, mental, and social well-being and not merely the absence of disease or infirmity’. WHO supports the idea about the integration of conventional and complementary practices to reach the best results for the patient and society [1] Traditional, Integrative, and Complementary Medicine practices are being adopted in several countries’ members of WHO. Acupuncture was the most common form of practice, closely followed by herbal medicines and indigenous traditional medicine. Homeopathy and traditional Chinese medicine came in next, each used by 100 Member States [2].
In 2006, Brazil the Health Ministry launched the National Policy of Integrative and Complementary Practices (PNPIC, MS ruling no. 971/2006) [3] with ensures to the population partial access to Complementary and Alternative Medicine. Under PNPIC, patients are provided with traditional Chinese medicine/acupuncture, homeopathy, botanicals, and herbal medicine cares free-of-charge, among others at Health Basic Units (HBU) and Family Health Support Units (NASF). Yoga was included in 2017 [4], and finally, apitherapy with the other 9 practices was included in 2018 [5]. According to the Health Ministry from Brazil, in 2019, 1,4 million of individual queries were done in these practices, with acupuncture in the leadership. Integrative practices are present in 9350 services and 3173 cities, from this 88% of the public health care system.
Considered the last frontier to be crossed in Hippocratic medicine, vibrational medicine aims to shed light on the understanding of the bodily system in its most subtle aspect of energy transference and systemic functioning regarding the physical-etheric interface of bodies. The Cartesian and mechanistic division of science and the world of the 20th century, which undoubtedly drove the great technological advance that the world finds today, as postulated by Einstein, Heisenberg, and colleagues, overcame this Newtonian fragmentation and led us back to the idea of unity, expressed in ancient Greece and Eastern philosophies [6].
Albert Einstein was one of the greatest physicists in recent history and, from him, there was the recognition that all matter is energy, and one can assume the understanding that the human body is a dynamic energy system. Energy and matter are two different manifestations of the same universal substance from which we are all formed, atoms. It is known that the movement between the constituent parts of the atom (electrons, neutrons, protons and smaller particles) forces that lead them, to generate energy. As the atoms are in constant motion, what can be measured is vibration. Each atom is unique because the distribution of positive and negative charges, combined with the speed of rotation, creates a specific vibration and a personalized frequency pattern [7]. The biophysical aspects of some therapies such as acupuncture, yoga, homeopathy among others, in general, are not many in publications in the area, due to the limited instrumental validation available.
The electromagnetic force is the fundamental reason for life. Atoms are sets of electrons and protons, molecules are sets of atoms, biopolymers are groups of macromolecules, and in the same way, life is an interactive congregation of biopolymers and macromolecules. The living being can then be considered an electromagnetic entity, which responds to a given electric or magnetic signal, as an expected result based on the laws of physics. Experiments have shown a level of electromagnetic organization in living organisms; intrinsic electrical characteristics and their sensitivity to external electromagnetic fields [8]. In this way, the human body can be considered a “resonance box”, capable of responding to various sources of stimuli (mechanical, sound, light, olfactory and tactile). Thus, everything that acts in its electromagnetic field or that comes into contact in the form of vibration in its physiological ensemble is liable to mobilize and be transformed into energy. It is from understanding the connection between the subatomic universe and physiology that we can understand how acupuncture, yoga, homeopathy, and why not apitherapy, that are capable of triggering various biochemical activities already mapped by science with their purely vibrational aspect. Considering the importance of the integrative and complementary medicine for health and aiming to contribute to the data already available in the scientific literature, this chapter aims to present some concepts about acupuncture, yoga, homeopathy, and apitherapy from the vibrational point of view, an important approach to understand the functioning of these impacting traditional medicines in human life.
Acupuncture, one of the tools of Traditional Chinese Medicine (TCM), in its more than 3000 years of existence, appeared intuitively in China, however it has had progressive scientific validation since the middle of the 18th century and, until today, it proposes to do the integration of the physical, psychic and spiritual bodies through the management of vital energy (
According to Lipton, the behavior of energy waves is important for biomedicine because vibrational frequencies can change the chemical and physical properties of an atom [11]. The knowledge of the links between the material body and its subtle form can enable a greater understanding of how it is possible to expand health in a preventive way in a global, affordable, and personalized environment [12]. We will not stick to the descriptions of the meridians paths, treatments, or explanation of the syndromes that are treated by acupuncture, but the biophysical events as a link that provides the effectiveness of the treatments of this ancient method.
According to Chinese philosophical thought, there is a concept of energy that permeates everything around us. It influences from the simplest form of life and even the movement of the planets, in a constant flux of renewal and expansion. This force/energy is part of the essence of the universe and involves all the entities that exist in it are called TAO [13]. In this philosophy there is a very particular view that correlates all the movements of the universe, macrocosm with the microcosm that represents everyone, in this way, everything is part of a great set. In this logic, the body is a macrocosm with countless microcosms, transforming and expanding with each movement of the individual. With this position, when contemplating a garden or a beautiful landscape, human being knows that the same energy that sustains and nourishes nature also exists within him; it manages to amplify its capacity for self-understanding and to value the importance of life cycles, confirms that it can establish itself in the face of the elements of existence [14]. From this point of view, the individual relates better and more respectfully to everything around him, perceives and contemplates the beauty of cycles, such as the arrival and the end of a season, harmoniously moving creation and destruction, and above all, he perceives himself to be actively acting in a macrocosm full of microcosms. Energy flows can be compared to the movement of the oceans. The currents have different characteristics, one is hot and fast, the other cold and slow, they vary according to the depth, and when they move they generate a lot of energy in this ecosystem [6].
In the millennial Huang Ti Nei Jing, known as Principles of Internal Medicine of the Yellow Emperor, we find the following statement: “the whole universe is an oscillation of
For the quality of life and health maintenance, acupuncture treatments provide information for understanding the circulation of vital energy (
In Oriental philosophy, the absence of health is seen as a momentary resource of the body in the search to reorganize itself, being a system of adaptation of this organism to anomalous environmental stimuli, pollutants, toxic agents, conflicts, and a decrease in vital energy.
The oldest written reference on acupuncture meridians is present in the book Huang Ti Nei Jing and contains precise descriptions of its principles and we can assume that it is the result of observing the beginnings of Chinese medicine. It contains information that, when stimulating the points on the map associated with certain organs or viscera (
Exploring the developments resulting from the relationships between them is not the goal of this chapter but understanding how the dermatomes connect with the meridians serves to complete the understanding of the invisible systemic tracks that run through the organism. To understand the vibrational vision in acupuncture, and which supports it scientifically, the first step is to know the studies that can validate this specialty over time, through the embryogenesis of acupuncture meridians.
It is known that meridians are a distinct morphological pathway. This finding was possible in humans through the findings of Frenchman Pierre de Vernejoul and his collaborators, who injecting metastable radioactive technetium 99 (99mTC) into the acupuncture points, observed the progression of the isotope in the mapped meridian lines, covering a distance of 30 cm in 4 to 6 minutes. When 99mTC was injected at random points on the skin, there was no similar result compared to the injection in the cuff [10].
Vernejoul based the work of Korean Kim Bong Han in the 1960s. By visualizing the path of the isotope phosphorus 32 (P32) injected into a rabbit’s acupuncture point, Kim observed the absorption of the isotope in a tubular system with about 0,5 to 1.5 μ (microns) in diameter with the use of micro auto-radiography; this path corresponded to the acupuncture meridian tracing of a given point; little or no activity was noticed in the meridional line when the isotope was injected into a vessel adjacent to the point. Through the histological study of these tubules, Kim’s group found that there were superficial and deep ducts that “floats” freely over the inside of the lymphatic vessels and vascular tissue, penetrating the vessel walls at specific points of entry and exit. The fluid present in these tubules moved in the same direction of blood and lymph flow and also in the opposite direction to them, which suggests that its origin is probably independent and previous chronologically to the embryogenesis of blood and lymphatic vessels.
According to him, as long as blood vessels develop, they grow around the meridians. The sequence of these findings was followed by the description of other tubular systems: The Intra-external Ducts, which appear to be arranged along the surface of Organs internal organs and flow independently from the blood, lymph, and nervous vessels. Superficial Ducts are found on the skin; and, finally, the Neural Ducts, distributed in the central and peripheral nervous system. All Duct systems are interconnected by Terminal Ducts of the various ductal systems and these reach the nucleus of the cell. Kim has also done numerous experiments that confirm the continuous flow of these ducts and the fluid contained in them. Within these ducts were found high concentrations of DNA, RNA, amino acids, nucleic acids, sixteen types of free nucleotides, adrenaline, corticosteroids, estrogen, and other hormonal substances at levels different from those found in the bloodstream. This indicated the path of understanding the interrelationship of acupuncture meridians and the regulation of the endocrine system [10]. Kim’s findings, according to Richard Gerber (2007), were associated with those of Yale’s neuroanatomist Harold S. Burr. Burr deeply studied the energy fields surrounding plants and living animals. He mapped the electric fields in the salamanders at an early stage of embryogenesis and found that the electrical axis that was aligned with the animal’s brain and spine originated in the unfertilized egg. Kim, consulting Dr. Burr’s findings, found that in the chicken embryo, the meridian ducts formed within 15 hours of fertilization. At that time, not even the most rudimentary organs were formed. With this, he can suggest that the functioning of the acupuncture meridian system influences the migration and the spatial orientation of internal organs. Burr and Kim proposed that meridians form a physical-etheric interface, where bioenergetic information and vital energy flow from the etheric body to the cellular level through these tracks, nourishing the bodily systems [10].
We can connect these studies with those of molecular biologist Bruce Lipton (2007), who, through a quantum perspective, reveals that the universe is a set of integrated and interdependent energy fields. The physical part and the energy fields that make up matter describes a non-linear or holistic flow of permanent emanation. According to him, the specific frequencies and patterns of electromagnetic radiation in the environment can regulate DNA, RNA, protein synthesis, alter the function and shape of proteins, control genes, cell division, their differentiation, morphogenesis (a process in which cells group together to form organs and tissues), hormonal secretion, growth and nerve functions [8]. In acupuncture treatment, the insertion of needles into acupoints, known in Chinese literature as the arrival of the sensation of
We have already seen that the meridians are not like a vascular system and neither are nerves, but they can be the neural and neurohormonal links between the central nervous system (CNS) and the peripheral nervous system, influencing it through the connective tissue when stimulated through their paths. In the nervous system, communication takes place through electrical action potentials. Information is transmitted through changes in the frequencies of action potential discharges. The rate of nerve electrical discharge per second generates a code, which will have a certain reading depending on the nerve that is communicating with the brain sensory region. The discovery that the systems made up of glial and Schwann cells - which previously only served to nourish the surrounding nerves also has the function of an electrical nature [10], is an important link for understanding this communication system physical-etheric. The most recent research indicates that the glial cell network can transmit information through slow changes in direct current potentials. It is possible that with the vibration of acupoint stimulation, which is characterized by a location on the skin with low electrical potential, an input is created in the nervous system, influencing the direct-current potentials of the glial cell network, which follows the path of the nerves [10]. When an action potential discharge begins in a nerve cell, a sequence of events is triggered, which passes through the entire sensory nerve fiber until reaching its synaptic ends. The electrical impulse, which carries a message to the brain, undergoes an energetic transformation in the synaptic cleft, which is converted into the release of neurotransmitters. The electrical potential in the cell membrane determines the reaction of each neuron to release neurotransmitter packages. Each nerve cell is in contact with many others forming a network, thus “spreading” the information for the modulation of the CNS and, consequently, the harmonization of regions that are unbalanced in the body system. It can be assumed in this way that the dual wave-particle behavior of subatomic particles is producing a set of “information” that, in essence, generates vibrations (waves) that seem to make the connection between the material and immaterial systems of bodies [12]. The acupuncture meridians and the nervous system operate in a complementary manner and, when adequately supplied with information and nutrition, they will promote higher energetic phenomena translated into harmonic cellular physiological patterns and also immunity, organized by the individual and unequivocal model of functioning present in each individual in the pituitary.
With the change in the energy environment of glial cells, the meridian system becomes capable of influencing the bioelectronic systems of growth and regeneration. We conclude with this that the effects of neurochemical releases, associated with changes in direct currents that are slowly transmitted along the perineural pathways, are not primary, but a secondary effect of the fluctuation of energy fields located in the vicinity of nerves and glial cells. Surrounding them from the vibrational stimulation of the needles in the dermis.
It is common knowledge that vibration triggers the action potential (AP) in neural cells, which originates through a disturbance of the resting state of the cellular membrane, with a consequent flow of ions, through the membrane and alteration of the ionic concentration intracellular and extracellular media [18]. Thus, the main cause of the resting potential would be the unequal distribution of ions in solution on both sides of the membrane, compartmented actively or passively by the selective mechanisms of transmembrane ion transport. The membrane, therefore, acts with a capacitor, storing energy in this spatial distribution of electrically charged ions; this potential electrical energy is available to be recovered quickly, in addition to stabilizing the membrane preventing this system from being disturbed by any minor factor. The aqueous medium fills most of the intracellular and extracellular spaces, and it is where almost all molecules (soluble, of course) interact to animate the intermediate metabolism, the mobilization of energy and nutrient sources, and the maintenance processes are suspended, and molecular and cellular repair [19]. The important fact is that all living cells have some differences in electrical potential between the cytoplasm and the extracellular space, being generally negative on the inside (resting potential of the cells). Some cells, however, can leave this situation of rest, propagating, throughout their membranes, disturbances that cause transmembrane ionic currents throughout the cell, and that can even invert the electrical profile concerning rest, even leaving it for some time the cytoplasm positive concerning the exterior: these are called excitable cells, and include neurons, muscle cells, and endocrine secreting cells. Some of these nerve endings release outgoing transmitters that tend to trigger an impulse; others are inhibitors and reduce the nerve’s tendency to fire. The impulse trigger will depend on the balance between the exciting and inhibiting influences of hundreds of synapses [15]. This mechanism and all known developments in the Western are responsible for the vibrational potential produced by acupuncture (Figure 1).
Neuroendocrine modulation from acupuncture stimulus (Source: Gerber, 2007).
The trigger above explains the
It is in the transformation of
The greater the understanding of the biophysical aspects of the energy generation, information, nutrition, and defense production processes in the body system, the better and more comprehensive the direction of integrative and complementary therapies in current and future integrative medicine will be.
Yoga is one of the six most important philosophies in India and to understand it from a vibrational point of view, first we will present some important concepts. As well as several other holistic knowledges, there are indications that Yoga has existed for at least 3000 years. It is a timeless philosophy that comprises a set of moral and ethical values, as well as some techniques to harmonize body, mind, and spirit. And although it is based on teachings contained in the “Vedas”, texts that form the basis of Hinduism, they have no religious connection. In Sanskrit, a language considered sacred in India, Yoga means to “unite”. It refers to the union of the being with the whole, through the study of oneself (self-knowledge) and the understanding that mind, body, and spirit do not separate.
For centuries, the teachings of Yoga were passed on orally, directly from master to disciple. Until, around 200 BC, a great Indian philosopher, “Patañjali”, codified them in 196 aphorisms, constituting what is known today as one of the most important texts of Yoga, the “Yoga Sutras” [20]. It should be noted that among these aphorisms, two of them, the III-53 and IV-33 deal with theories where the nature of time and matter would be discontinued, in line with current discoveries of quantum physics, scientifically proven, that irradiation of light is not continuous [21]. Patañjali describes eight steps that the Yoga practitioner must follow. The first two stages,
The “Yogis” believe that the physical body is only one of the five different bodies (layers), which constitute the being and that must be in perfect harmony to reach the state of totality. The five bodies, or
According to this philosophy, there is an energy originated in the Sun, which penetrates the Earth’s atmosphere through the sun’s rays and is responsible for all life on the planet. This energy is called Prana and has been reported by different peoples around the world for millennia. They gave these energy different names, but it is the same vital energy. In China, it is known as
The sacred texts also speak of 72,000
The analogy between these two different structures, one based on neurophysiology and the other on a tradition of ancestral wisdom, has been the focus of several scientific studies. They aim to understand how this complex and extensive energy network is related to the nervous system, endocrine glands and blood and its relationship with the health-disease process [27, 28, 29, 30, 31, 32].
Neuropsychiatrist S. Karagulla deepened her research on the interaction of the etheric body and
It is also worth mentioning the research carried out by Motoyama, Ph.D. in Philosophy and Clinical Psychology, which tends to confirm the existence of
Another relevant study was carried out by Dr. Valerie Hunt, which were used electrodes electromyography devices connected to telemetry equipment, to capture changes in the skin, in the region corresponding to the location of the
According to the Yoga philosophy, the principle of everything that exists in the universe is propagated through vibrations. Thus, it is believed that thoughts, words, feelings, food, and everything else that exists on this planet, has a vibratory pattern that can affect people’s health in a positive or negative way.
The balance between the five bodies and the healthy functioning of the
Recently, scientific studies have been published to assess the benefits of
The vibrational benefits of Yoga are also promoted by chanting
Another technique widely used in Yoga, are the
The five fingers of the hands represent the five elements. Fire (
There are several reports on the “powers” acquired through the practice of Yoga. Motoyama recounts his experiences in the book “Theory of the
However, Yoga is a philosophy that uses techniques to manipulate and harmonize vital energies, unlock the channels and energy centers, encouraging the practitioner to (re)connect with the vibration that exists in everything. Induces the practitioner to disconnect from the external agitation and dive into the internal vibration, becoming aware of the energetic reality emerging in their bodies and minds, and thus, letting go of
Homeopathic therapy was created by the German physician Samuel Hahnemann in 1796, due to his dissatisfaction with the therapy used at that time. Hahnemann made his proposal for this new therapy after seeing that the symptomatic picture of malaria, which had quina bark (
This concept had already been presented by Hippocrates, considered the father of Medicine, who describes that “the disease is produced by the similar and, through the similar, the patient returns to health”. Although this proposal was previously presented, it was Hahnemann who was responsible for demonstrating it clinically, based on his fundamentals, endowed with information in the Experimental Medical Matter and with an exclusive Pharmacotechnics [47].
In addition to the presented principle that “the similar heals other similar”, Hahnemann introduced the concept of minimum doses, explaining that these were chosen after their effectiveness was noticed, since, by reducing the concentration of substances through their dilution, the same continued to have a therapeutic effect, without harming the symptoms of intoxication [48].
The art of studying the toxic effect of substances on animals already existed, but it was necessary to make a similar study in man, so that Hahnemann developed this experimentation, elaborating the homeopathic medical materials from these observations. To explain this fundament, the father of Homeopathy quotes in his book considered being the greatest work [49],
Another basis of homeopathy presents the proposal of using a single medicine, capable of covering the entire symptomatic of the individual, which is, a medicine whose pathogenesis best coincides with the manifestations presented by the patient, this being his simillimum [47], that is, a medicine that when used, will act considering the organism as a whole, having all its integral parts, in order to re-establish the harmony of the system.
From his experiences, Hahnemann proposed a therapy based on the vital force, explained by him in the 6th edition of Organon [50] by the words
And then, according to the principle of Vital Force, under the Law of Similars proposal, and following the fundamentals of experimentation in a healthy man, minimum doses and single medicine, it was published by Hahnemann, in 1976, in the Journal of Practical Medicine, an article in which states that “disease can be cured by drugs that cause symptoms similar to the disease” [47].
To exemplify the law of similars, Bellavite et al. [49], presented some schematic representations of two examples of the application of the law of similars and of diluted and dynamized substances, in inflammatory models. The example presented was obtained with the medicine
The scheme presented represents the results of Poitevin et al. [50] and were based on the use of bee venom (
As shown in Figure 2a and b, the sources of the medical material
a - Activation of normal basophil degranulation caused by IgE antibodies. b - Inhibition of basophil degranulation by the use of homeopathic drugs (ultra-diluted and dynamized) such as
The mechanisms by which Hahnemann’s proposal is based is not yet clearly demonstrated, and most likely due to the technological limitation for such energetic measures involved to be made. A look from a vibrational point of view may shed light on a possible way in which homeopathic medicines act, especially when you think about the concept of a single medicine and its fundaments that the similar heals other similar.
Homeopathic medicines come from a solution or mother tincture produced from products of plant, animal, or mineral origin. All of these have atoms, protons, electrons, neutrons and smallest particles, and for that reason, they are in constant vibration, and the energy/matter duality, just like humans, having the premise of Einstein’s theory, in which matter is composed of particles, which ultimately consist on light/energy, in the wave/particle duality. Analyzing the Hahnemannian point of view on the vital energy, someone could suggest that the vital energy is the vibration that needs to be preponderant for the state of health. When diseases change this vibrational frequency, they need something that offers the dosage of energy/frequency necessary for this vibration return the original vital energy.
In the process of health and illness, it could be said that the individual’s energetic/vibrational state has been altered, and in order to restore the original energy, a vibrational energy medication compatible with that necessary for the restoration of the individual’s vital energy, is necessary. Thus, and taking as a reference the homeopathic process of dynamization of the medicines, which follows a dilution and a succussion (rhythmic shaking of the bottle containing a medicine), aiming to awaken the medicinal energy of the substance, in water, and considering that the raw materials of departure have their own vibration, it is possible to suggest that homeopathic medicines, already without quantifiable matter by chemical methods, considering the number of Avogadro, have only the vibration of the original substance, the subtle energy, which will exercise its function of the ethereal subtle energy of the sick individual.
One hypothesis is that the drug energy of a substance that causes a symptomatic picture identical to that provoked by the disease, presents the same frequency as it, and thus, the frequencies cancel each other out, returning the vibration of the normal individual to preponderate. It is not uncommon in the homeopathic healing process, the individual presents an aggravation of the symptoms, and this could be the consequence of the initial summation effect of the medication vibration, added to the energetic picture of the disease, which highlights the symptomatic picture to stimulate the immune response to restore the individual’s energetic/vibrational state of balance.
After the Albert Einstein presentation about the revolutionary theory of energy and matter, using the very famous equation: E = mc2, which establishes the quantitative equivalence of the matter’s transformation into energy or vice versa, and the comprehension of each atom is composed by electrons, neutrons and protons that are under constant vibration, emitting energies with different frequencies, and that humans are, in the last level, composed by atoms (equivalent to energy), it is much easier to understand that each individual possesses your own vibrational standards. When this vibrational standard state of ‘normal’ level is modified by one disease, something needs to happen to re-establish the energy to the ‘normal level’ again (Vital Energy,
To clarify this statement, imagine the electrons that are in specific space regions known as orbitals. Each orbital presents a specific characteristic of energy and frequency, according to the atom and its molecular weight. In order to change the position of one electron to another next superior orbital, it is necessary to transmit the energy of a certain frequency. Only one quantum of exact energy necessary will stimulate the jump of the electron to the next superior orbital. Through the resonance process, the energy of appropriate frequency will excite the electron and cause it to pass to the highest energy level in its orbit around the nucleus. Maybe human behavior could be compared to the electron change of energy level, if we consider (as an analogy) the existence of ‘orbitals’ of health and disease, for example with different vibrational levels (vibration/frequency). In the case of human beings whose energetic systems are in a disease orbit, only a subtle energy dose with the appropriate frequency can be accepted and cause the body to move into a new orbit or steady state of health. Vibrational remedies could inject the quantity necessary of subtle energy in the human system through the induction of resonance. This etheric energy changes the vibrational level of disease to the orbital of health [10].
The energy that gives life is called ‘vital energy’ in homeopathy, ‘prana’ in yoga and
During this chapter, it was possible to present until now, 3 different types of treatments, all based on vibrational medicine, with different techniques: acupuncture, yoga, and homeopathy. Considering all this information, now it is time to talk about apitherapy and how bees can help during the health process.
Apitherapy or “bee therapy”, from Latin
Ancient civilizations with their millennial therapies recognize and use the bee products as valuable resources in their medical practices. The history of medicines of the Chinese, Tibetan, Egyptian and also the Greco-Roman civilizations are quite rich, containing in its records, dated for a long time (more than 6000 years in ancient Egypt), hundreds of recipes, including among the main ingredients, honey, propolis, bee larvae and eventually the bees themselves to cure or prevent diseases [53].
Hippocrates (460–370 BC), Aristotle (384–332 BC), and Galen (130–200 AD) prescribed the use of honey and bee venom as a cure for baldness. Hippocrates recognized the healing virtues of bee venom for treating arthritis and other joint problems, also used propolis for healing sores and ulcers internally and externally. Today growing scientific evidence suggests that various bee products promote healing by improving circulation, decreasing inflammation, and stimulating a healthy immune response [51].
Nowadays, apitherapy is adopted in some countries as Complementary and Integrative Medicine with a specific regulation, as is the case of Brazil for example [5], but some others deserve special attention since they possess an important tradition in the apitherapy use and/or Organizations of Apitherapy running some job in each country in this field. In Brazil, honey and propolis possess widespread use by the population. Apis Flora Company introduced propolis extract in the Brazilian market at the beginning of the 80s, and from this moment, several other innovations appeared as the natural syrups based on mixtures of honey with propolis and others bee products, and/or with herbal extract and/or essential oils, vaporizers with the same type of mixtures. Extracts, lozenges, tablets, capsules, and others can be found in European, American and Asiatic countries, and they can be found in pharmacies, drugstores, natural product houses, supermarkets, etc. [54]. Besides honey and propolis, royal jelly, bee pollen, and apitoxin can also be found, however with less expression than the firsts.
For understanding the apitherapy importance as integrative medicine and in order to propose some connection with vibrational medicines, it could be useful to present some small information about what each bee product is, its traditional use, composition, and benefits according to clinical trial and/or systematic review available until now. As the literature about each bee product is quite large, and the deeper description about “in vitro” and “in vivo” protocols about mechanisms involved already done, are not our focus in this chapter, only some information will be provided in order to try to propose an innovative way about thinking apitherapy with vibrational medicine information. But first, let’s know something about the vibrational communication between bees.
As could be seen in the text published by Scheider & Lewis [55] the vibration signal produced by bees is used as a modulatory communication signal, as it is involved in several actions executed in the beehive. The frequencies, high or low, the pulses short or long, besides other characteristics in the vibration not only activate but also are involved in the stop of some functions. Von Frisch [56] compared this warning sounds with the vibrations produced by honeybees during “buzzing dances” and the buzzing tones. Stop signals are short, high-frequency signals that occur under rather different conditions. Nieh [57] described that short pulses of body vibrations were performed by foraging bees, offering a warning to the others about a dangerous food experience. The literature available proposes that different signals communicate different things, activating, or stopping actions. Not surprisingly, many different terms have been used to characterize these different short pulses (e.g., piping, begging, buzzing, shaking, whooping).
Besides the communication actions developed, Schneider, Huang & Lewis discovered that the juvenile hormone titers of workers 15–30 min after receiving vibration signals are slightly, but significantly higher than those of non-vibrated control bees that are matched for age, location in the nest and initial activity levels [55]. One parallel that it is possible to do in this topic is that the evidence already described the higher concentrations of some hormones like adrenalin, estrogens, corticosteroids, besides others, in the meridians used as key points in acupuncture, in comparison with the concentration in the blood, show evidence about the connection of these points with the body endocrines glands [10]. So, the vibration modified by the application with the needles in the acupuncture in these meridians can modify the endocrine glands’ behavior, affecting some physical behaviors in the body. Here it is possible to meet some convergence with what vibration provoked by bees can do in the production of some hormones of other bees.
It let us consider that vibration produced by bees can stimulate higher production of some compounds in the bee organism. And the question that one could do is: Can vibration produced by bees energize bee products produced in the beehive as royal jelly, bee venom, honey, propolis, etc.? Is there some characteristic vibration of bee products that can affect the etheric vibration of the humans? Of course, this hypothesis needs to be validated, but let’s do an exercise with bee products in order to evaluate this hypothesis.
Honey has been used in the human diet since 25,000 years ago when the first tangible demonstration was found [58]. Besides its use for nutrition and mainly as a sweetener, its use as a traditional medicine in several cultures worldwide was very well documented [59, 60, 61]. The use of honey for therapeutic purposes dates back to Egyptian papyrus, and it was also mentioned in Chinese medicine and Hindu documents [62].
The chemical composition of honey is based on a supersaturated sugar solution, especially rich in fructose and glucose, which also present more than 181 substances containing minor quantities of minerals, vitamins, phenolic acids, flavonoids, enzymes, organic and amino acids, proteins, and a huge variety of essential oils according to the different flowers visited by honey bees [63, 64]. The honey composition will never be the same since it depends on the source-type, soil, climate, and genetic factors and procedures methods involved. In this sense, the feeling and sensation promoted by honey as colors, flavors, smells, and tastes difficult will be the same [65, 66].
Several biological properties were already demonstrated for honey as antioxidant action that contributes to the prevention of several diseases as cardiovascular problems, cancer, diabetes, and others, especially by the protection against free radicals and other oxidative substances [67, 68, 69]. Besides antioxidant action, antimicrobial and wound healing are other two properties very well recognized in the literature and it is mainly attributed to high osmolality and sugar concentration, low pH, aromatic acid, volatile substances, and peroxides composition [70, 71, 72, 73]. Antiviral and fungicide activities were also demonstrated and linked to some flavonoids found in honey as chrysin, acacetin, and apigenin [74].
Riera et al. evaluated the Cochrane systematic reviews and protocols for complementary medicine in order to check several new therapies approved in Brazilian regulation. These studies demonstrated that honey dressings for partial healing of wound burns and honey to reduce coughing among children with acute coughs were effectively able to demonstrate scientifically and with the scrutiny of rigor science, the important safety and efficacy of honey (26 randomized clinical trials, with around 3100 individuals), supporting apitherapy introduction in the therapies proposed by the Brazilian Government [75].
Although several biological actions under Newtonian methods were already demonstrated, to think about the influence of the vibrations produced by bees when they are communicating with each other (“buzz”) and its impact on the bee products under vibrational level, the question could be: could honey be ‘carrying’ the energies of the flowers that were visited by bees during the collection of nectar and pollination? Flowers as the humans are composed of atoms, protons, electrons, and neutrons, and also, vital energy, and consequently, also possess their own frequencies. Besides it, flowers are rich in essential oils, volatile compounds that can be energized during the flights of the bees with the vibrational movements and sounds. Could honey be acting in the healthy restoration by modifying the etheric Humans Vibration? (in the level of Vital Energy,
The word propolis means ‘in defense of the city’, and it is derived from Greek
The literature already demonstrated several important biological activities for propolis as an antioxidant, anti-inflammatory, immune modulator, antibacterial, antifungal [76, 77, 78, 79, 80] and several many others [76]. Propolis was evaluated in some clinical trials with important good results in preventing respiratory tract infections in children in a randomized, double-blind, placebo-controlled, multicenter study [81], as a supplement as an adjuvant in asthmatic patients [82] and as a prophylactic for immune stimulation [83].
As it happens in the case of honey, propolis also is variable according to the region, botanical sources, climate, etc. that bees collect the exudates to transform into propolis. This was an important remarkable point for regulatory agencies such as the European Medicines Agency (EMA) to criticize the approval of propolis as a medicine, due to the lack of standardization [84]. In this sense, it was presented in 2012, a propolis standardized extract called EPP-AF® [79]. Propolis EPP-AF® was already studied according to its safety and efficacy in animal and human protocols for several applications [79, 85, 86, 87, 88, 89]. Of remarkable interest was the clinical trial done with EPP-AF® (500 mg/kg/day) in kidneys damage protocol during 12 months of research and monitoring of elderly patients, with impacting results in the reduction of proteinuria biomarkers [90]. Also, and not less impacting was the demonstration of the lack of interaction evaluated in a clinical study with hepatic enzymes CYPs and the transport protein PgP. Propolis EPP-AF® demonstrated safety at this level using WHO protocols [91]. Finally, Berretta et al. [80] published a review article supporting the use of propolis to reduce the damages and aggressiveness of SARS-CoV-2 in the COVID-19 patients. But, besides the Cartesian mechanisms already demonstrated above to propolis effects, could propolis also act by vibrational medicine? After looking forward to finding some toxicity data in animal and clinical trials for propolis, no impacting results were found, except by sparse cases of an allergic reaction [92, 93]. Some patients can be allergic to propolis due to a topical application, inhalation, or ingestion. Curiously, diluted propolis applied by inhalation reduced allergic airway inflammation system [94], besides the several studies demonstrating that propolis possesses anti-inflammatory results in several models with a huge range of dosages. In this example about allergy, Does the small dosage inhaled, could be acting following the principle of homeopathy? Could propolis be “energized” by the vibration produced by bees? This is another hypothesis to be tested if we can attribute vibrational effects to apitherapy.
But, if we consider that propolis was produced by bees after the collection of plant exudates, rich in chemical substances as flavonoids, phenolics, essential oils, etc., that possess vital energy and a vibrational standard, and suffer vibration during this process, could we postulate the hypothesis that propolis could be acting in the frequency modification on an etheric level as a vibrational medicine? Newtonian evidence already demonstrated the pharmacological effects, but could something more be happening?
Some European countries as Germany and Slovenia are using as apitherapy beehive air inhalation (“apirespiration”) to promote the health of respiratory systems. The inhaled aroma produced in beehives has been suggested to be beneficial to the health of human beings [95]. The best results usually were related to immune disorders such as allergies, chronic sinusitis, and susceptibility to infections, for patients of all ages [96].
In the beehive environment, the air is constantly circulating due to the vibration of the wings of thousands of bees. The air is saturated with water vapor, particles of isoprene, terpene, essential oils, hormones, feromones, liquid wax, alcohol, bee saliva secretions, propolis, trace elements, enzymes, choline, phytohormones, etc. By natural respiration, these substances are applied in the human body, having a positive effect on various diseases, whether as prevention, cure, or stabilization [51]. “Apirespiration” sum the benefits of the substances produced in the beehive with the vibrational energy of the flight of the bees, associated with the breathing of the pleasantly warm bee air (around 36°C). Excellent results of apirespiration have been proven with bronchitis, asthma, allergies, diphtheria, chronic inflammation, impaired immunity, migraines, and depression [51].
Several systems were proposed to this type of apitherapy, as a respiratory system connected directly to the honeycomb (Figure 3) and others completer and more planned in well-structured rooms were already suggested, the ApiHouses.
Apirespiration system.
The inhaled aroma produced in beehives has been suggested to be beneficial to the health of human beings. This integrative medicine is still an incipient field of study, and research projects in rich countries have improving its current results despite the inherent difficulties by the high number of bee species and consequently the quantitative and qualitative differences in the chemical composition of the honeybees and the concentration of their by-products (Figure 4).
Some ApiHouses proposed by [
The different models and classifications used in each case for ApiHouses were related to different kinds of experience. Some more related to the api respiration properly said, the inhalation of the volatile substances and the vibration offered by bees, but also the interaction and peace obtained with the vibration of nature and its sounds.
In the cases of ApiHouse, the question could be if the vibration/sounds produced by bees were affecting the etheric vibration of the patient modifying it to the ‘normal’ level, or if the vibration was “energizing” the volatile substances emitted by the beehive, or both.
Bee venom importance was highlighted with the publication of the First scientific paper by Desjardins, a French physician on the successful treatment and curative properties of bee venom for rheumatic disease. Filip Terc (1888) was considered as the “Father of Apitherapy”, he applied around 39,000 bee stings to over 500 rheumatic patients, was the first one to use systematically this type of therapy culminating with the publication “Report about a peculiar connection between bee stings and rheumatism” [96].
Bee venom is produced by bees for two glands (acid and alkaline glands) associated with the sting apparatus of worker bees [96]. It is a complex mixture from honey bees, which possesses various peptides including melittin, apamin, adolapin, apamin and mast cell degranulation peptide, enzymes (PLA2, hyaluronidase, acid phosphomonesterase, α-D-glucosidase, and lysophospholipase), biologically activity amines, and non-peptide components such as histamine, dopamine, and norepinephrine [97].
Bee venom therapy can be applied in some different ways, (i) live bee sting, (ii) bee venom injection, and (iii) bee venom acupuncture. The scientific data suggests that bee venom acupuncture is more effective than the application of live bee sting, bee venom injection, and acupuncture alone [96, 97]. The sum of bee venom and acupuncture offers the benefits of the chemical and pharmacological effects of the substances found in the bee venom and the mechanical application in the acupoints [97].
The number of studies already published with bee venom is too large. In order to show some application of its bee product, some interesting results will be present. Curiously, bee venom and acupuncture are very connected. Khalil et al. [98] demonstrated that bee venom acupuncture at Yanglingquan acupoint (GB34) improved locomotor behavior significantly, reduced several central amines, reduced pro-inflammatory cytokines, and neuronal apoptosis, demonstrating strong evidence of a neuroprotective effect of this therapy. Bee venom and acupuncture also demonstrated good results in idiopathic Parkinson’s disease [99]. In rheumatoid arthritis, the effects occur beginning with an anti-inflammatory action, reducing the expression of some cytokines and the level of glucocorticoids, protease activity, and reactive oxygen species (ROS) [97].
Besides the innumerous animal studies with bee venom, only some clinical trials were already performed, however, with a relatively small number of patients, for the lumbar disc disease treatments, knee osteoarthritis, rheumatoid arthritis, adhesive capsulitis, lateral epicondylitis, neuropathic pain, Parkinson disease, stroke, depression. An interesting mini-review about the use of bee venom under the point of view of safety and efficacy in acupuncture puts the question “to bee or not to be” in this topic, since the risks about the allergic reactions can be very high and so, a good evaluation of the ‘costs’ and benefits needs to be done in order to take this decision - “To bee or not to be, is that question” [100].
Now, under the point of view of vibrational therapy, definitely, the tiny quantities of the bee venom necessary to produce the effect let us know about the effect of homeopathic medicine, especially thinking about the potential toxicological effects. Would the Bees vibration offer a homeopathic characteristic to this product? The high incidence of successful results applying bee venom in the acupoints really calls attention. The substances present in bee venom, in a tiny concentration, can offer different pharmacological types of effects, including important damages. The mechanical application of needles in specific acupoints can stimulate vital energy
Other bee products could be presented here as royal jelly, bee wax, bee pollen, however, with the examples presented above it was possible to know something about apitherapy and its importance for humanity, and open the mind to think about new possibilities for the influence of bee products in living beings. If these hypotheses are true, we will only know if appropriate techniques are used. So far, it is only speculation to be tested.
It is noted that, although more comprehensive, the new concept of health adopted by the World Health Organization is still incomplete, not considering the “individual” in its entirety, which includes the vibrational and energetic aspects addressed in this chapter. Biologists have long been impressed with the ability of living things to maintain their own stability. The idea that a disease is cured by natural powers, by “
Starting from the essential unit of matter, these authors presented the biophysical links in common and specific methods for the search for homeostasis. The search for the integral man, full of its capacity for self-regulation, goes through the path of effective and progressively more natural preventive health.
Launching a vibrational look at ancient therapies (acupuncture, yoga and apitherapy), as well as for the youngest among them, homeopathy, represents contemplating and renewing the ancestral bonds that connect the three parts of the human being: body, mind and spirit. An innovative way of think in this sense were presented for homeopathy and some new hypotheses were formulated, especially regarding the function of bees as a vibrational instrument for each bee product known.
It is possible that many readers will be concerned, because the larger the body of evidence, the more it will be possible to provoke a closer look within the countless therapies presented in this book. The greater the understanding of the biophysical aspects of the energy generation process, the distribution of information, the use of nutrition for the production of defense in the body system, the more accessible and comprehensive the integrative and complementary therapies in current and future medicine will be.
The authors would like to express their gratitude to Apis Flora Indl. Coml. Ltda. for financial support to this chapter and for all efforts in demonstrating the therapeutic effects of propolis and other bee products. And also to be thankful to the QuantaVita Systemic and Oriental Therapies, Ribeirão Preto/SP, Brazil, for collaboration as a space for active observation of vibrational medicine and for providing the fundings to the lead author of this chapter (JMMG), and to CAPES (Coordination for the Improvement of Higher Education Personnel - Brazil) that provided the scholarship to the author (MCFR).
We also thank all the researchers’ morphic fields that inspired the authors with their work and hypotheses. To the masters who, since immemorial times, have contemplated nature, experienced its benefits, and experienced its assumptions. Without them, this chapter would not be possible. Thanks to the family systems we belong to, thanks to them and their evolutionary efforts, we have produced these links for an increasingly inclusive complementary medicine.
The authors declare that they have no conflict of interests.
WHO | World Health Organization |
PNPIC | National Policy of Integrative and Complementary Practices |
MS | Ministério da Saúde/Health Ministery |
HBU | Health Basics Units |
NASF | Family Health Support Units |
TCM | Traditional Chinese Medicine |
CNS | Central Nervous System |
AP | Action Potential |
SP | Spleen and Pancreas meridian |
LU | Lung meridian |
K | Kidney meridian |
L | Liver meridian |
HT | Heart meridian |
TE | Triple Burner meridian |
SI | Small Intestine meridian |
LI | Large Intestine meridian |
EMA | European Medicines Agency |
EPP-AF | Propolis Standardized Extract – Apis Flora® |
CYPs | Cytochromes |
PgP | Glicoproteína-P |
PLA2 | Phospholipase A2 |
SARS-CoV-2 | Severe acute respiratory syndrome |
COVID-19 | Coronaviruses |
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