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",isbn:"978-1-83969-467-7",printIsbn:"978-1-83969-466-0",pdfIsbn:"978-1-83969-468-4",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,hash:"a6f32d3f2227df637fffd969a0cb5ed7",bookSignature:"Dr. Peter A. Clark",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/10878.jpg",keywords:"Preimplantation Genetic Diagnosis, Medical Futility, Definition of Death, Extraordinary/Ordinary Means, Need for New Antibiotics, Role of Big Pharma, Uterine Transplants, Face Transplants, Confidentiality, Ethical Decision Making, Harm Reduction Theory, Safe Injection Sites",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"February 8th 2021",dateEndSecondStepPublish:"March 8th 2021",dateEndThirdStepPublish:"May 7th 2021",dateEndFourthStepPublish:"July 26th 2021",dateEndFifthStepPublish:"September 24th 2021",remainingDaysToSecondStep:"7 days",secondStepPassed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"A faculty member for medical residents, medical students, and undergraduate students and a researcher in issues that challenge the national and global arenas. 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From chapter submission and review, to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"69220",title:"Nanoplasmonic Arrays with High Spatial Resolutions, Quality, and Throughput for Quantitative Detection of Molecular Analytes",doi:"10.5772/intechopen.89064",slug:"nanoplasmonic-arrays-with-high-spatial-resolutions-quality-and-throughput-for-quantitative-detection",body:'The optical properties of metal nanostructures and nanoarrays have been widely investigated in the context of exploiting particle plasmons to derive high performance within chemical and biosensing devices. These include sensing of range of analytes to diagnose the medical status of an individual [1]; detect the presence of chemical or biowarfare agents [2, 3], toxins, or adulterants in food [4]; or assess/monitor air, water, and soil quality in the environment [2, 5]. Plasmonic sensors offer a range of advantages over other analytical tools including high analytical and calibration sensitivity, quick response times, label-free detection opportunities, ease of integration within different sensor form factors, and the need for simple and portable instrumentation. Plasmonic sensors rely on metal nanostructures that act as nanoantennae to concentrate and enhance the electromagnetic field close to surface [6]. The enhanced EM field can be leveraged within different configurations, namely, localized surface plasmon resonance sensors (LSPR) that follow analyte-induced changes to local refractive index or report vibrational Raman or fluorescence intensities with high signal-to-noise ratios using surface-enhanced Raman spectroscopy (SERS) and metal-enhanced fluorescence (MEF), respectively. In all these cases, the performance of the sensor is critically linked to the optical properties of the plasmonic structures, which in turn correlates with their geometric attributes, namely, size, shape, aspect ratios, separation, distribution, and roughness. Control over geometries is the key to engineering profiles and intensities of the electromagnetic field around plasmonic nanostructures, which in turn determines the performance of the plasmonic transducer. The typical length scales for characteristic dimensions of plasmonic nanostructures correspond to spatial resolutions of few nanometers to few tens of nanometers. Geometric features such as pointed structures or nanoscale gaps have shown to concentrate and enhance EM field, thus acting as EM hot spots, as a function of decreasing radius of curvature or gap distances, with length scales down to sub-10 nm regime [7]. Enhanced EM fields at gap hot spots have shown to result in enhancement factors of the order of a million- to billion-fold in practice. A sizeable contribution to observe SERS signals (24% of the overall intensity) was observed to be rising out of <100 molecules per million when these molecules are positioned in EM hot spots with EM enhancement factors of the order of 109–1010 [8]. The EM hot spots help enhance the sensitivity of different plasmonic sensors, and especially those based on enhanced spectroscopies, namely, SERS and MEF. Gaining high sensitivity would thus be largely determined by the quality and number of EM hot spots [9]. However, addressing the production of EM hot spots with length scales of the order of only a few nanometers pose a profound and non-trivial challenge for nanofabrication. Traditionally, such hot spots have been attained as a natural consequence of stochastic growth or deposition processes [10, 11], including electrochemical growth or roughening, de-wetting of nanoparticles or salts from solution phase [12, 13, 14], and de-wetting of thin films on the surface. The stochastic processes, by nature, result in a broad standard deviation in geometries, which reduces the number of most efficient hot spots and also leads to greater spot-to-spot variability in signal intensities [15, 16]. The randomness in geometry makes it particularly hard to predict response, to identify the source of issues, and to adopt a rational approach to optimize performance. Geometries with improved definition have remained forte of top-down lithography tools, for example, E-beam lithography, focused ion-beam milling, and X-ray interference lithography, which are all very time-consuming and also quite expensive [17, 18, 19]. The throughput and cost of fabrication is not only an issue for manufacturing but also reduces the efficacy of research due to the limitation in the number of samples available for investigations [20] (Figure 1). From the manufacturing perspective, the throughput of the process would be a key determinant of the cost, which is driven to <$5 per sensor chip for point-of-care applications [28].
Schematic representation of the trade-off between resolution, quality, and throughput in fabricating plasmonic nanoarrays with high spatial resolutions, showing the comparative advantages and limits of (a) nanoparticle assemblies [21] (b) top-down-bottom-up control over nanoparticle assemblies [19], (c) direct-write techniques [22], (d) nanostencil lithography [23], (e) nanoimprint lithography [24], (f) photolithography [25], (g) porous anodized alumina templates [10], and (h) stochastic, random structures, including island films [11, 26], and (i) stochastic chemical growth processes [27].
Techniques such as nanoimprint lithography and polymeric or colloidal self-assembly techniques allow enhancing throughput, albeit, at the cost of defects. The ability of self-assembly techniques in catering to the parallel fabrication of nanopatterns across arbitrarily large areas at low cost, as well as the several handles it offers toward tunability of structure dimensions down to molecular level, is unmatched by conventional lithography tools. However, self-assembly-based approaches carry limitations that demand careful attention to ensure their usefulness: (a) Poor uniformity, which is often a result of poor process optimization and in some cases due to the susceptibility of process parameters to environmental variables. This can be addressed by mapping the impact of environmental variables and factoring them within the process optimization. (b) Large standard deviations, either inherent to the primary templates produced by the technique or those that may creep in during different stages of processing. (c) Feature shape: Typically, self-assembled template patterns have a circular feature cross section. Rectangular, triangular, or other feature shapes with lower symmetry are uncommon. (d) In the absence of any external guidance, self-assembly techniques typically lead to a polycrystalline 2D hexagonal order. Long-range ordering, square, or other lattice types besides hexagonal symmetry remain uncommon and can be attained through guidance from a top-down lithographic tool [29, 30, 31, 32, 33, 34]. The lack of long-range order and presence of point or line defects are better tolerated by plasmonic sensing applications, so long as the averaged properties are consistent and reproducible with low standard deviations. The chapter will present our approach to plasmonic nanoarrays with high spatial resolutions relying on hierarchical self-assembly of amphiphilic di-block copolymers into soft colloids and their subsequent quasiperiodic organization when deposited on a planar surface [35]. The approach results in well-defined organic templates on the surface with nanometric control over the width, topography, and pitch, realized by control over parameters of molecular self-assembly. By understanding the impact of the different process parameters on the resulting geometric outcome, it is possible to deliver templates with high reproducibility and uniformity on full wafers, with a yield >90% [36]. These templates are translated into highly sensitive SERS-based plasmonic sensors, with control over metal nanogaps down to sub-10 nm regime.
Amphiphilic di-block copolymers can self-assemble into reverse micelles when dissolved in a solvent that selectively dissolves only the apolar block of the copolymer. These reverse micelles can be obtained or be induced into attaining a spherical morphology, with a feature size determined by their aggregation number. The micelle aggregation number is not only a function of the molecular weight and composition of the copolymer but also the quality of the solvent used and the presence of additives. As a consequence, the size can be varied independent of the molecular weight, using solvent quality and concentration of additives as useful handles to fine-tune template geometries and eventually those of resulting plasmonic arrays. The spherical micelles are solvent-laden colloidal structures which can be readily organized on a variety of surfaces to yield a 2D hexagonally ordered dot-array templates. These templates are subjected to physical or chemical means of pattern transfer to produce nanopatterns of desired materials. Pattern formation on surfaces using copolymer reverse micelle approach is fundamentally different from that of microphase separation in block copolymer thin films [37, 38, 39]. The size of reverse micelles in solution, which eventually determines the feature sizes on the surface, is independently variable by engineering the solvent quality or the use of additives. The standard deviation of the templates on the surface is determined by that of the micelles in solution phase before deposition, which in turn is governed by the intermicellar exchange process. The exchange process is slow due to the slower diffusion for larger molecular weight polymers or when solvent with high selectivity to the corona-forming blocks is employed [40]. During the spin-coating process, the solvent-laden micelles in solution deform on the surface to assume an ellipsoidal shape, with partial fusion of corona from adjacent reverse micelles resulting in the globally continuous organic film presenting periodic contrast in topography with an ultrathin film (<5 nm) in the background. The pitch of the ensuing pattern on the surface can be varied in steps <5% of its mean value, through control over evaporation rate or the concentration of the micelles in solution. The evaporation rate can be controlled using spin or dip coating speeds. The ability to vary the lattice periodicity within a certain window is attributed to a range of distances for which the PS blocks from the corona of adjacent micelles can still meet upon deformation and film formation. When this condition is not met, namely, at low solution concentrations or high spin speeds, the reverse micelles are spaced too far apart, resulting in patchy coverage. On the other hand, at high solution concentrations or at low spin speeds, the excess concentration beyond what is necessary toward a monolayer appears as multilayers [36]. The topography of the reverse micelle film is a variable that can be determined by the relative humidity in the ambient environment during the coating process. This is attributed to the change in moisture that is likely to concentrate at the polar core-forming PVP and consequently increasing the interfacial tension resulting in resistance to collapse and as a result a higher topography. Under optimal conditions of coating, micelle arrays can be produced with a standard deviation of <10% in geometric attributes across a complete wafer.
In a specific example shown in Figure 2, reverse micelles of polystyrene-block-poly (2-vinylpyridine) (PS-b-P2VP) are obtained from dilute solutions of m-xylene. Here, a copolymer with a molecular weight of 81.5 kDa and a PDI of 1.10, at a concentration of 0.5% w/w in m-xylene, is spin-coated on a clean silicon surface at 5500 rpm resulting in a hexagonally ordered array with feature heights of 20 nm and pitch of 66 nm with typical standard deviations <15% in all geometric variables. During the spin-coating process, the solvent-laden micelles in solution deform on the surface to assume an ellipsoidal shape, with the corona from adjacent reverse micelles coming together resulting in the globally continuous thin film presenting periodic topography and ultrathin film (<5 nm) in the background. The center-to-center distance or the pitch of the template arrays could be systematically decreased in steps of <5 nm between 45 and 60 nm by increasing the solution concentrations from 0.6 to 1% at a fixed spin-speed of 5000 rpm or decreasing spin-speeds from 9000 to 2000 rpm at a fixed concentration of 0.7%. The ranges were found to constitute an optimal window of conditions where a continuous uniform film was obtained.
Schematic representation of the self-assembly of amphiphilic di-block copolymer into reverse micelles and their subsequent assembly to form two-dimensional arrays on the surface.
Among key limitations encountered by self-assembly-based approaches, in general, is the scaling up to practically large areas while ensuring high consistency and reproducibility. The issues of reproducibility arise mainly due to the sensitivity of the process outcome to environmental parameters. Such sensitivity also limits process scalability, due to inconsistencies encountered when coating large areas like full wafers and to limited batch-to-batch reproducibility. These issues are true also for the case of self-assembly of amphiphilic copolymers, and this can be addressed only by adequate investigations directed at mapping the impact of different environmental variables on the process outcomes. Several sources of variability were identified and addressed for the self-assembly of amphiphilic copolymers, including the presence of moisture and contaminants in solution; history of preparation (agitation and incubation), temperature, and humidity; differences in surface roughness or surface energy (e.g., due to organic or particulate contaminants on the surface); changes to solution concentrations due to solvent evaporation during use; and inadequate mixing of polymer. Under optimal conditions, the assemblies of reverse micelle feature exhibit standard deviations lower than 15% across full wafers.
A specific outcome of optimization of the templates represented in Figure 2c on a 100 mm silicon wafer can be seen in Figure 3, which shows the distribution for height, diameter, pitch, and nearest neighbors, at different regions of the wafer. Typical characterization involves AFM topography for heights and diameters (within errors of AFM tip-convolution) and SEM top view and image analysis using ImageJ or MATLAB. A representative AFM image recorded in the tapping mode is shown in Figure 3b. The characterization is critical for each batch of samples, and it is possible to scale the process to several batches of wafers [41]. Points A, B, C, and D correspond to four positions representing systematically increasing radial distances from the center to the edge of the wafer. AFM measurements at these points show standard deviation <15% for geometric variables and <10% variation of their mean values across the full wafer. Voronoi analysis of the AFM images shows a predominance of six nearest neighbors as expected for hexagonal packing, which is uniformly maintained across the wafer (Figure 3e,f). The outcome clearly demonstrates the feasibility for reliable scaling up of the technique to cater to nanostructures over large areas.
Demonstration of scalability combined with uniformity on full wafers of optimized coatings: (a) Schematic of radially separated points from center to edge of 100 mm wafer, (b) where tapping mode AFM measurements are performed (image indicated for point a), (c) the distribution of height and pitch across full wafer, (d) with mean values plotted as function of radial distance from center, and error bars showing standard deviation in corresponding feature dimensions at a single point, (e) histogram of nearest neighbors showing predominantly six nearest neighbors as expected for hexagonal assembly, maintained across the wafer, and (f) representation of nearest neighbors using Voronoi analysis, with the features colored corresponding to the number of nearest neighbors as indicated in (e).
Fabrication of plasmonic nanoarrays starting from organic templates relies on pattern transfer approaches, for example, template-guided growth, deposition, or etching, to define noble metal nanoarrays with the conservation of pitch from the original template. The pattern transfer approaches offer large flexibility in the geometry of the individual features, thus making it possible to fabricate plasmonic nanoarrays of different types, for example, nanoparticle cluster arrays, nanopillars arrays, nanorods, or nanodiscs. The pattern transfer parameters provide independent control over the size, shape, and aspect ratio of the features and should be optimized to ensure that they do not affect the spatial arrangement, uniformity, and reproducibility from the original template. Although the pattern transfer approaches are common in semiconductor fabrication, extending them to work at the scale of few nanometers requires rigorous optimization and quality assurance to ensure low standard deviations and reproducibility in geometries. Further in this section different pattern transfer methods to reach such three different plasmonic nanoarrays have been discussed in detail (Figure 4).
Fabrication of plasmonic nanoarrays with different profiles, size, and distributions of metal nanostructures can be attained by control over pattern transfer processes. In all cases, the advantages of the original templates including the uniformity and scalability are preserved [42, 43, 44].
Clusters (used interchangeably with “aggregates” in this report) of metal nanoparticles behave differently from their isolated counterparts due to the collective optical behavior arising out of plasmonic coupling between the constituent nanoparticles [45, 46, 47, 48, 49, 50]. Clusters of nanoparticles are known to behave as hot particles, with significantly enhanced electromagnetic fields at the inter-particle junctions [51, 52, 53, 54]. Consequently, clusters exhibit higher extinction cross sections, with hot spots that can be excited at lower energies than the isolated particles. The aggregation-induced color change of gold nanoparticle suspensions caused by the analyte of interest has been the basis of several biological assays [55]. Such random aggregation typically results in a large distribution in the number of particles per cluster, with a distinct lack of control over those numbers. To achieve clusters with desired optical properties, it is essential to be able to produce them with a narrow distribution in the size, shape, and spatial arrangement between nanoparticles within the cluster and between clusters in an array. Such clusters were demonstrated using templates fabricated by electron- beam lithography (EBL) [45, 46, 47, 53, 56, 57, 58] and controlling the size of the template to obtain dimers, trimers, quadrumers, and multimers. Since EBL is time-consuming and expensive to achieve high-resolution patterns spanning large areas, other low-cost means to achieve controlled nanoparticle aggregates using template-assisted means have been reported in the literature. These include the use of DNA, [59] surfactants, [60] block copolymers [60, 61, 62], carbon nanotubes [61], cylindrical micelles [62], or microorganisms like bacteria or viruses [63] as templates to attach nanoparticles. While the template-assisted cluster formation allows creating clusters with desired size (or a number of particles per cluster) and shape, they often fall short of abilities to control inter-cluster arrangement within an array. Such an arrangement is important to ensure reproducible inter-cluster plasmonic interactions as well as an ability to engineer them to achieve desired optical properties. In this direction, block copolymers are an excellent solution, as they allow effective control over the spatial arrangement of eventual clusters. Microphase separation of block copolymers (BCP) in thin films, as well as BCP reverse micelles, has been utilized as templates to attain clusters of metal nanoparticles on surfaces. These techniques have exploited the BCP templates either to organize one or more particles from solution phase [64, 65, 66] or to drive selective complexation of metal ions and in situ reduction to form clusters [67]. However, these fall short of opportunities for the rational design of the cluster properties. The approach based on copolymer reverse micelle template allows preparing clusters based on electrostatic self-assembly of preformed gold nanoparticles from the solution phase on to the features on the surface. The cluster dimensions (the number of nanoparticles per cluster) and the inter-cluster separation were controlled by control over the template size and their separation. The low standard deviation of the template enables low standard deviation in the geometric attributes of the clusters as well (Figure 5).
(a) Schematic of the fabrication of gold nanoparticle cluster arrays by electrostatic attraction of negatively charged Au nanoparticles to positively charged templates on surface, (b) zeta potential titration showing the template acquiring positive potential for pH below 8.3, (c) systematic control over the size (pitch kept a constant), and (d) separation (Sc) between the nanoparticle clusters (size kept a constant at N~18) which is shown by the histograms and AFM measurements, respectively [42].
In a typical experiment, the reverse micelle arrays prepared from PS-b-PVP with a molecular weight of 114 kDa and PDI of 1.1 were spin coated from m-xylene solutions and subsequently exposed to an aqueous suspension of gold nanoparticles. Due to the presence of the pyridyl groups of PVP in the core of the features, the reverse micelle features are positively charged and attract negatively charged citrate-stabilized gold nanoparticles from the suspension. The electrostatic attraction between the nanoparticles occurs locally on the features and not in between them, therefore resulting in a patterned array of nanoparticle clusters. The number of nanoparticles in each feature is a function of its size. The inter-cluster separations are controlled by the control over the pitch of the template. Extinction spectra of the nanoparticle clusters showed a peak at 620 nm, which was over 100 nm red-shifted from the plasmon resonance band of isolated Au nanoparticles. This is attributed to the strong plasmonic coupling between the nanoparticles within the cluster. Further, the absence of a strong contribution at 520 nm would confirm the geometric observation of the absence of isolated nanoparticles (Figure 6). Finite-difference time-domain (FDTD) simulations show inter-particle as well as inter-cluster hot spots. The 3D shape of the clusters was found to be necessary for the inter-cluster plasmonic coupling, and an increase in EM field at inter-particle and inter-cluster hot spots was found to correlate with decreasing inter-cluster separations. The expectations from the geometry and optical properties of the nanoparticle cluster arrays were validated by SERS measurements of a probe molecule. Three different probe molecules were tested, namely, crystal violet, naphthalene thiol, and 2, 2′-bipyridine. In all three cases, the SERS intensities were found to be highest for the largest clusters with the smallest separations. The SERS intensities were higher for crystal violet than for smaller molecules due to resonance Raman effects, enabling SERS enhancement factors over 108. SERS quantitative assays showed the lowest detection limits for naphthalene thiol to be an order of magnitude lower than 2,2′- bipyridine. This is attributed to the higher density of molecules for naphthalene thiol on the surface due to the covalent bonding of the thiols to the gold surface at a given concentration. The low standard deviations of the cluster arrays enabled signal intensity variations below 10% on several square millimeters on the surface.
(a) Graph shows the shift in the extinction peak of the nanoparticle clusters with change in separation, Sc, and change in no. of nanoparticles/cluster, N. (b) SERS spectra of CV shows the increase in the peak intensity at 1625 cm−1 with change in the separations, Sc, and no. of nanoparticles/cluster, N. (c) and (d) SERS intensity vs. concentration showing linear relationship for 1-NT and BPE, respectively [42].
Nanopillar shapes offer a unique opportunity to control and enhance EM field profiles and enhancements [68]. Nanopillar arrays for plasmonic sensing have been reported previously in the literature, for detection of disease markers and identification of bacteria as well as environmental pollutants [43, 69, 70, 71]. Such configurations can be obtained by metal deposition of optimal thickness on top of high aspect ratio dielectric pillars that can be produced by different approaches, namely, VLS growth [72], pulsed laser deposition [73], black Si production by RIE with gas plasmas [74], and patterned wet or dry etching with templates produced by other means, for example, photolithography, soft-lithography, EBL, NIL, NSL, and molecular self-assembly [75, 76, 77, 78, 79, 80]. Nanopattern of pillars offers an advantage over stochastically arranged counterparts due to a better definition of the spatial relationship between the pillars that help rational enhancement to sensing performance. The stochastic arrangements, despite promising results, rely largely on empirical optimization and suffer from difficulty in identifying issues when they arise. Another aspect to consider is the stability of the pillar arrays, which is especially a concern for high aspect ratio structures [81, 82, 83, 84]. High aspect ratio pillars can capture analyte between the pillars when they collapse by drying that may offer interesting means to trap analyte potentially at EM hot spots [85, 86]; the approach, however, gives less control over the collapse nor the concentration of analyte that is trapped, thus offering limited opportunity for rational design. The irreversible nature of such collapse would make it difficult to subject the pillars to solvent-based washing and drying steps, limiting them to a single time or single-step usage. Pillars of smaller aspect ratios would solve many of these issues,; however, it will need to be produced with high spatial resolutions to ensure a large number of EM hot spots and preferably with good geometric definition allowing rational optimization toward both enhancing EM hot spots and analyte capture on the surface. An approach based on colloids of amphiphilic copolymers allows the possibility to generate nanopillars that are stable and reusable while allowing significant opportunities to rationally engineer the optical properties toward high plasmonic sensing performance.
Organic reverse micelle templates can be transferred into the underlying Si by adopting nanolithography processes based on reactive ion etching with halogen gas plasmas. Given the low template thickness (typically less than 30 nm) and small widths (sub-100 nm), nanolithography using these templates requires significant process optimization to ensure high selectivity to the underlying substrate, minimum undercut, and high anisotropy. It is possible to substitute the organic templates with a harder inorganic template to improve the selectivity and durability. This is achieved by transferring the organic template pattern into a thin dielectric film to generate harder dielectric masks for the next step of pattern transfer into Si. We had earlier shown a high degree of control over the incorporation of the organometallic precursor by using atomic layer deposition, with the resulting oxide nanostructures enabling nanolithography down to sub-10 nm regime [35]. This was followed by several other investigations in literature focusing on vapor phase incorporation of metal-organic precursors within different block copolymer domains using ALD processes [87, 88, 89, 90, 91]. It is important that the nanolithography process is optimized to not widen standard deviations between the features or across the wafer. The optimization processes across the full wafer level should take into account the “loading effects,” which impacts the outcome of the reactive ion etching process depending on the proportion of exposed surface area available for etching.
Nanopillar arrays that are shorter and closer allows all advantages of the other reported approaches, while in addition also conserving the metal to be deposited. Nanopillar arrays of silicon obtained by molecular self-assembly approach (Figure 8) can subsequently be coated with gold or silver to prepare plasmonic nanopillar arrays. The conditions of the coating, including the choice of evaporation versus sputtering, the respective deposition parameters are critical to the structure and property of the resulting plasmonic arrays. The thickness of the films deposited in relation to the geometry of the underlying silicon pillars would be a key process parameter that determines the aspect ratio and the separation between the metal pillars. Both aspect ratio and the feature diameters increase, while the feature separations decrease systematically as a function of the thickness of metal deposited. The observed film growth is strongly anisotropic with slow growth laterally as compared with vertically. The growth on top of the silicon pillars correlates well with the thickness of the metal deposited, while the increase in diameter occurs much slower. The challenge in the processing includes the conformal deposition of metal, with good step edge coverage, especially when the deposition is made on top of closely separated high aspect ratio nanopillars.
In a specific example, organic templates prepared using PS-b-PVP with a molecular weight of 80.5 kDa was spin-coated on a 100mm Si wafer consisting of a 25 nm film of thermally grown SiO2 layer (Figure 7a). The coating is exposed to brief O2 plasma to remove the thin residual layer between the template features and expose the substrate beneath. The resulting organic template is then transferred into an underlying SiO2 thin film using C4F8/CH4 gas plasma. The resulting SiO2 islands provide high selectivity in etching underlying Si using SF6/C4F8 plasma to yield Si nanopillars. The RIE conditions employed resulted in silicon pillars with a positively tapered profile with a feature size of 40 nm (at half-width) and pitch of 78 nm and height of 120 nm, as measured by SEM (Figure 7b). The RIE process conditions can be varied to obtain pillars with other shapes as well (Figure 7c). The pillar arrays were found to be uniform throughout the wafer, as qualified by uniform color, with a variation of <10% as measured by reflectance spectroscopy [35]. Figure 8 shows another case of similarly obtained nanopillars arrays prepared using a different PS-b-P2VP (114 kDa, PDI - 1.1, fPS ~0.5) system. The Si pillar arrays are converted to plasmonic nanoarrays bycoating with thin layer of Cr, followed by systematically increasing thicknesses of gold by electron beam evaporation. The nanopillars coated with 5 nm Cr/120 nm of Au were found to result in gold pillars with separations below 10 nm. The optical modeling of the nanopillars proved EM hot spots with increasing intensity and with increasing metal thickness, as expected. This was correlated well with SERS experiments of 1-naphthalene thiol, where the evolution in SERS intensity was found to saturate at 100 nm. SERS-based plasmonic assays of naphthalene thiol show analytical sensitivity down to 74 ppb, low standard deviations in SERS intensities for different concentrations, and feasibility for quantification with large dynamic range. The approach established clear control over structure, property, and function to optimize the final performance of the plasmonic sensor.
(a) Fabrication process involved in pattern transfer from polymer template to fabricate silicon nanopillar arrays. (b) SEM image taken from a random position on the wafer with nanopillar arrays. (c) SEM images of different shapes of nanopillars obtained by varying etching conditions [35].
(Top) Silicon nanopillar arrays with increasing thickness of gold (top, insets) show evolution in electromagnetic field profiles (bottom, left) and SERS spectra of 1-naphthalene thiol as function of metal pillar geometry (bottom, right) concentration dependence of intensity of the peak at 1371 cm−1 showing quantitative detection with low detection limits [43].
The uniform templates obtained using reverse micelle approach is well-suited as masks for nanolithography to produce Si nanopillar arrays. These nanopillar arrays are highly interesting for exploitation as high-resolution molds for nanoimprint lithography (NIL) [44]. NIL is a convenient top-down patterning tool that allows replication of surface relief structures down to sub-10 nm feature sizes present in a mold into the polymer substrate [24, 92]. Replication using the NIL process is achieved by pressing the mold against a molten polymer film, followed by solidifying the polymer either by cooling below its Tg or by cross-linking, before removing the mold. Among the several top-down techniques known, NIL has particularly recognized as manufacturing compatible, scalable and low-cost solution for fabricating nanoscale templates of high resolution. The use of the silicon nanopillar arrays as NIL molds offers a distinct advantage of producing multiple copies of templates with identical pitch and width as the original pillars. Since these templates are used to produce metal arrays in the next step, it is possible to attain asymmetric metal features, with reproducibility in optical properties within and between samples. In a specific example, full wafers consisting of Si nanopillar arrays with a height of 120nm were fabricated as described in Section 3.1. They were subsequently diced into smaller pieces and used as NIL molds to replicate the pillar arrays into a thin film of PMMA (100 nm thick) coated on Si substrate. Prior to imprinting, the pillar arrays are functionalized with a perfluorosilane layer to enable anti-stiction property. The NIL process results in a nanoporous PMMA film with geometric characteristics that match with the Si pillar array mold (Figure 9). The pores were found to be ~120 nm deep, with a residual layer of ~10 nm thickness present beneath the pores. The residual layer was removed using a controlled O2 plasma exposure and treated with HF in order to expose the bare Si substrate beneath. The porous template with through-holes was subsequently employed to guide the growth of metal to achieve gold nanorods from the surface through an electroless deposition process. The electroless deposition of gold was performed by galvanic displacement reaction where the oxidation of silicon substrate by HF provides electrons to reduce the Au(III) ions to Au(0), in a process earlier shown by Aizawa et al. [93, 94]. Exposure of the nanoporous template to an electroless plating bath consisting of 0.9% HF and 2.3 mM of HAuCl4 for a duration of 1 min was sufficient to grow the nanorods within the pores. The selectivity of the process to the Si substrate ensured the absence of any non-specific metal deposition in unintended areas. The nanoporous PMMA template was then removed using an O2 plasma exposure. The Au nanorod arrays were found to be 80 nm in height with a pitch identical with that of the NIL mold used. Upon annealing the nanorod array at 200°C for 2 h, a transformation in morphology into nanodiscs was observed. Such transformation that was observed to occur significantly below the melting point of bulk gold metal is likely due to compacting of nanoparticulate and porous gold features obtained upon electroless deposition. The nanoparticulate nature of nanorods is evident from the SEM measurements. The nanodiscs exhibit an ellipsoidal shape, with a diameter of 55.2 (± 4) nm and height of 35.3(± 5.4) nm, as measured using SEM and AFM. TEM cross section of the nanodisc arrays shows that the discs were present within depressions on the surface with a depth of ~5 nm. The depression below the nanodisc is presumably formed by substrate etching, due to the presence of HF in the electroless chemical bath. The transformation of the rod to disc morphology was found necessary in order to ensure mechanical stability of the metal arrays when exposed to solvents. It was found that the nanorod arrays disintegrated when dipped in aqueous solutions, while the nanodiscs remained undisturbed. Since our study primarily intended application of these arrays within a SERS-based sensor in liquid media, the nanorod arrays could not be considered further. Despite this instability, the nanorods could still find a use for plasmonic sensors functioning in the gas phase, or alternatively, if their mechanical stability can be improved through deposition of an overcoating of a dielectric, for example, alumina as shown earlier in literature [95]. The nanodisc arrays were evaluated for SERS performance against three different molecules, namely, naphthalene thiol that represented a covalently bound analyte, 2,2′-bipyridyl that represented non-covalently bound analyte, and crystal violet which represented the possibility of resonance Raman effect. The SERS performance was benchmarked against commercially available Klarite substrates and was proved better for all three molecules. The approach demonstrated an inherently reproducible approach to the fabrication of plasmonic arrays, as several copies can be derived starting from the same nanopillars NIL stamp. The performance of these arrays nevertheless has scope for improvements by further EM enhancements by reducing feature separations. The inability to deliver low feature separations is a limitation of the approach, which can be overcome by post-processing of the porous templates obtained after NIL, for instance, via pore-widening approaches.
Process steps involved in production of (a, b) high-resolution molds for NIL produced from self-assembled polymeric templates, (c, d) followed by replication onto PMMA thin film to form nanopore arrays and (e, f) selected area growth of gold nanorods within the pores and their subsequent transformation into nanodiscs [44].
Molecular self-assembly using amphiphilic copolymers and colloids derived thereof can deliver nanoplasmonic interfaces with high spatial resolutions, with control over geometric variables in steps of only a few nanometers. The approach enables metal nanoarrays with spatial coherence between features, orthogonal control over the different geometric attributes, and standard deviations below 10%. These characteristics can be leveraged to better understand and predict the optical properties of these arrays, allowing rational routes to maximize plasmonic sensing performance. The self-assembly parameters at both the template production and pattern transfer stages could be rigorously controlled to ensure high uniformity, reproducibility, and scalability of the resulting plasmonic arrays on full wafers. The correlation of the geometry ⇔ optical ⇔ SERS performance was demonstrated with a combination of experiments and numerical simulations. Plasmonic nanoarrays presenting a large number of gap hot spots, with gap distances down to sub-10 nm length scale, are possible to obtain in case of nanoparticle cluster arrays and nanopillar arrays. The homogeneously distributed hot spots over large areas present an opportunity to not only detect but also quantify the concentration of analytes, with large dynamic range with promisingly low limits of detection. Among the key challenges for future developments is to identify configurations that naturally drive the co-localization of analytes with EM hot spots to achieve maximize plasmonic signal enhancements. Further, the efforts to enhance EM fields solve only a part of the sensing challenge. In addition to maximizing the EM enhancements, the surface needs to be tailored to maximize analyte interactions and their concentrations on the surface.
Yet another challenge is the application of plasmonic arrays for biosensing. The high spatial resolutions sought for maximizing EM enhancements at gap or curvature hot spots are not compatible with the spatial requirements to accommodate large biomolecules like proteins. Further, the sensitivity of the plasmonic sensor extends typically to only a few nanometers from the surface. This is a challenge considering that the size of biomolecular interactions can already be a few tens of nanometers, for example, for an immunosandwich assay. Further, the plasmonic sensor needs to be adapted to work in complex media, for which the surface functionalization to avoid non-specific binding would also consume part of the sensitive space above the plasmonic interface. While the plasmonic sensors have held high promise for highly sensitive, fast responding, and portable configurations, they need significant transversal development cutting across topics beyond fabrication, physics, and photonics, to ensure reliable devices that address emerging analytical challenges.
Funding received from National Research Fund of Luxembourg (FNR) via the project PLASENS (C15/MS/10459961) and FNR-PRIDE (FNR PRIDE/15/10935404) is gratefully acknowledged.
Postharvest spoilage of fruits can be caused by a large number of bacterial species. Some of the most important are Enterobacter cloacae, Erwinia herbicola, Lelliottia amnigena, Pantoea ananatis, Pantoea agglomerans, Pantoea allii, Enterobacter aerogenes, Pseudomonas fluorescens and Streptomyces sp. [1, 2, 3, 4, 5, 6]. A wide range of fungal species is similarly involved [2, 7, 8, 9].
\nIf adequate postharvest handling and storage practices are not employed, postharvest decays of fruit and vegetables can cause losses of 50% or more [7]. The main triggers for invasion by microorganisms are physiological changes that activate ethylene synthesis or that cause changes to the cuticle or cell walls (loosening), or declines in natural antifungal compounds or high contents of carbohydrates and other nutrients and water. These changes usually occur naturally during ripening [10, 11, 12].
\nPostharvest contamination of fruit by human pathogens can be another key issue in the supply chain. The most commonly reported human pathogen contaminants causing disease outbreaks are bacteria such as Escherichia coli (E. coli), Salmonella spp., Mycobacterium spp., Brucella spp. and Pseudomonas aeruginosa (P. aeruginosa). However, good manufacturing and handling practices can significantly reduce these contaminations [13, 14].
\nBecause of the behavior of microbial populations, including fungi and bacteria, an initial infection may originate new infection foci that appear near the primary one, so increasing disease incidence and/or severity [15, 16]. Quality deterioration and loss of fresh fruit and vegetables during storage have an exceptionally high economic impact because by this stage high costs have been incurred in harvesting, grading, packaging, freighting and storage. All these reasons emphasize the importance of defining new practices to reduce populations of the postharvest microorganisms.
\nSilver nanoparticles (AgNPs) offer oligodynamic action which is also of low toxicity and broad spectrum [17, 18, 19]. Moreover, compared with synthetic biocides, there is also only a low chance that microbial resistance might develop. These AgNPs have been exploited against Gram-negative bacteria, such as Acinetobacter, Escherichia, Pseudomonas, Salmonella and Vibrio, and against Gram-positive bacteria including Bacillus, Clostridium, Enterococcus, Listeria, Staphylococcus and Streptococcus [20]. A number of research reports have demonstrated that their antimicrobial nature depends on the surface-capping agent and the size and shape of the nanoparticle [21, 22].
\nThe effectiveness of AgNPs also depends on bacterial dose [23]. Silver nanoparticles affect the growth of bacteria in a dose-dependent manner. In a study conducted by Agnihotri et al. [23], concentrations of 10 and 20 μg/ml Ag (10 nm) caused reductions of ~18 and ~53% in E. coli, respectively. Meanwhile, AgNP concentrations at 30 and 40 μg/ml eliminated all bacterial growth.
\nSilver nanoparticles smaller than 100 nm, and containing between 10,000 and 15,000 silver atoms, are effective as antibacterial agents [20]. The AgNPs’ antibacterial potential increases as size decreases. This effect is more pronounced for AgNPs of size <10 nm, because contact with the bacterial cell is direct [24].
\nResearch into the antimicrobial activity of AgNPs against Gram-positive and Gram-negative bacteria shows Gram-negative bacteria are more sensitive to AgNPs than Gram-positive ones [23, 25], although their relative sensitivity cannot be explained based only on a difference in the composition of the cell membrane.
\nIn studies using discs impregnated with AgNP in culture media with bacteria, the formation of a clear zone of inhibition around the impregnated discs is an indicator of bactericidal potential of AgNP > 15 nm [21]. Bacteria are unable to survive in this area, possibly because of the release of silver in the form of nanoparticles or of silver ions.
\nIn addition, nanoparticle silver can be released by the mobility of small size AgNPs through the semisolid agar, whereby a zone of inhibition is observed.
\nIn a previous study conducted by Biao et al. [21], chitosan was combined with silver nanoparticles to form composites. They found that chitosan-silver colloid has a high inhibition ratio against the prokaryotes E. coli and Staphylococcus aureus (S. aureus) and the eukaryote Candida albicans (C. albicans). They concluded that the chitosan-silver colloid had a broad spectrum of antimicrobial activity.
\nA way to transport active silver cations to the bacteria can occur on the cell membrane or within the cell. When combined with protonated chitosan, the positively charged AgNPs bind well to the negatively charged bacterial membrane proteins through electrostatic attraction [23].
\nThe first bacterial contact with AgNP can trigger an antibacterial mechanism by facilitating the entry of AgNPs into the bacterial cells. This is followed by an explosive release of silver ions inside the bacterial cells causing the bactericidal effect.
\nThe nature of the AgNP, bacteria interaction and its antibacterial effect have been analyzed by a number of methods. Bacteria exposed to AgNPs show high protein leakage and morphological changes [26]. As an example, E. coli treated with AgNPs (∼10 nm) appeared to shrink and develop an irregular shape. Micrographs show AgNPs on the cell membrane attached to the lipopolysaccharide layer of the cell wall, and a proportion of AgNPs were found inside the bacterial cell [23].
\nBiao et al. [21] noticed that bacterial strains have intact membranes and smooth surfaces in the absence of silver colloid, whereas after exposure to chitosan-silver colloid, the cell membrane and surface become shriveled, invaginated and disrupted. This cell membrane damage indicates the mode of action of chitosan-silver colloid. Its bactericidal effect is attributed to the release of silver cation from AgNPs and to alteration of the bacterial cell wall structure and associated physicochemical changes.
\nOsmoregulation of the bacterial cell can also be affected causing extrusion of intracellular material and hence cell death. The deformed or wrinkled cell wall can also cause leakage of cytoplasmic contents.
\nIn addition, AgNPs can penetrate bacterial membranes, facilitating internalization. The rupture of perforation of the cell wall is an evidence of internalization of AgNP and of uncontrolled transport through the cytoplasm resulting in cell death [27] (Figure 1).
\nMode of action of silver nanoparticles in the bacterial cell.
Multiple pathways of AgNP can occur after internalization. Silver atoms in nanoparticles are characterized by a high affinity with sulfur and phosphorus-containing compounds such as DNA. In this way, they readily combine with cell constituents and so destroy the cell.
\nSilver ions can also inhibit bacterial replication by binding and denaturing bacterial DNA. Silver ions react with the thiol groups of enzymes, followed by DNA condensation resulting in cell death [28, 29].
\nBlocking of respiration is also a result of the interaction with cell membranes [30].
\nDisruption of biofilms is another effect of AgNPs. The anti-biofilm action of ~8 nm AgNPs on Gram-negative bacteria has been demonstrated [31]. The outer membrane of Gram-negative contains aquaporins (water-filled channels) which are involved in the transport of Ag ions into the cell where they exert their antibacterial effects [32].
\nFlow cytometry (FCM) is a well-established and powerful analytical tool that has led to many revolutionary discoveries in cell biology and cellular-molecular disease diagnosis and, more recently, has been used to analyze physiological responses of bacteria [33, 34]. In FCM, cells are first introduced to a high-speed (up to 5–20 m/s) laminar flow stream, and after being focused into single file, they are subjected to laser-induced fluorescence, and/or forward and sideways scattered light is detected using photodetector arrays with spectral filters. More recently, FCM has been used to characterize distinct physiological conditions in bacteria including their responses to antibiotics and other cytotoxic chemicals [33]. Once the control of bacterial cells or fungal conidia has been applied, an accurate technique is required to measure the effectiveness of the silver nanoparticles. Flow cytometry is one of the most reliable techniques for detecting and counting living cells and to measure their viability.
\nWhen studying response to antibacterial agents such as silver nanoparticles, viability can be evaluated as an indicator of antibiotic susceptibility. There are now reagents available that allow assays of membrane permeability and potential by measuring the production of a fluorescent metabolite from a nonfluorescent precursor [33, 34].
\nBesides monitoring susceptibility to antibacterial activity, information can be obtained using FCM that can establish mechanisms of antibacterial drug activity [35, 36, 37, 38, 39, 40]. Traditional culture-based techniques cannot do this [41]. The use of fluorescent probes to detect specific cell changes provides a unique tool for interrogating bacteria permeability and changes in membrane potential [42] (Figure 2). DNA content and metabolic activity [42] are useful indicators of cell viability and thus of antibiotic susceptibility.
\nFluorescent probes to detect specific bacterial cell changes as an indicator of cell viability.
The accuracy of cell counting depends on fluorescent staining. The choice of a fluorescent dye should take into account factors such as membrane permeability, photostability, pH and sensitivity to temperature [43, 44]. The total bacterial count is a key quality criterion for food or beverages [45] and a useful tool for detecting the presence of microbes within matrices. Williams et al. [46] used this technique to detect E. coli O157:H7 in raw spinach. The presence of plant pathogens during crop growth has been investigated by several authors. Day et al. [47] used FCM to detect and quantify Phytophthora infestans sporangia. A study of colonization of root-associated bacteria in rice was carried out by Valdameri et al. [48]. Otherwise, Golan et al. [49] counted Pectobacterium carotovorum subsp. carotovorum cells tagged with green fluorescent protein (GFP) in Ornithogalum dubium seedlings to detect resistant cultivars. The application of FCM is useful to create the bases for predictive models of spore germination, infection and disease development.
\nCell viability assays can distinguish between live and dead cell populations and so correlate with other cell functions or treatments. Many companies offer a wide range of viability dyes, including fixable and non-fixable types and ones specific to bacterial or yeast viability tests. FCM can be applied to monitor the efficacy of treatments to reduce contamination of water [43] and foods and beverages [45, 50] by determining the viability of residual microorganisms. In agriculture FCM can be used to test the effectiveness of antibiotics and antifungals against plant pathogens. The advantage of live FCM cell counts compared to plate counts is that FCM allows the determination of several different morbidity stages between living and dead cells. Some of these are membrane integrity, esterase activity, membrane potential, electron transport, total cells, GFP expression, active/dead, mitochondrial activity, intracellular pH and carotenoid content [51, 52, 53].
\nThe Food and Agriculture Organization of the United Nations predicts that, globally, about 1.3 billion tons of food is lost per year. A large proportion of this loss is caused by postharvest microbial action. Much of this loss could be averted if more effective procedures and protocols were developed and adopted. Nanotechnology offers a range of novel tools with application in the fight against microbial food spoilage. Silver nanoparticles can act at cell level affecting from the cell wall or finely affecting the DNA. They offer a viable alternative to more traditional methods for the bacterial control. Once bacterial control is achieved using silver nanoparticles, continual bacterial monitoring becomes a critical component of the supply chain. For this, flow cytometry offers an accurate, novel and versatile technology through which to survey bacterial viability in assays of various bacterial control strategies.
\nWe are indebted to Carolina Alvarado Gonzalez for the artwork.
\nThe authors declare there is no conflict of interest regarding the publication of this chapter. This chapter has not previously been published and is not being considered for publication elsewhere. The authors certify that neither the manuscript nor its main contents have already been published or submitted for publication in a scientific journal.
\nIntechOpen implements a robust policy to minimize and deal with instances of fraud or misconduct. As part of our general commitment to transparency and openness, and in order to maintain high scientific standards, we have a well-defined editorial policy regarding Retractions and Corrections.
",metaTitle:"Retraction and Correction Policy",metaDescription:"Retraction and Correction Policy",metaKeywords:null,canonicalURL:"/page/retraction-and-correction-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\\n\\n1. RETRACTIONS
\\n\\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\\n\\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
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\\n\\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\\n\\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\\n\\n3. CORRECTIONS
\\n\\nA Correction will be issued by the Academic Editor when:
\\n\\n3.1. ERRATUM
\\n\\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\\n\\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n3.2. CORRIGENDUM
\\n\\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\\n\\n4. FINAL REMARKS
\\n\\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\\n\\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
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\\n\\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
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\\n"}]'},components:[{type:"htmlEditorComponent",content:'IntechOpen’s Retraction and Correction Policy has been developed in accordance with the Committee on Publication Ethics (COPE) publication guidelines relating to scientific misconduct and research ethics:
\n\n1. RETRACTIONS
\n\nA Retraction of a Chapter will be issued by the Academic Editor, either following an Author’s request to do so or when there is a 3rd party report of scientific misconduct. Upon receipt of a report by a 3rd party, the Academic Editor will investigate any allegations of scientific misconduct, working in cooperation with the Author(s) and their institution(s).
\n\nA formal Retraction will be issued when there is clear and conclusive evidence of any of the following:
\n\nPublishing of a Retraction Notice will adhere to the following guidelines:
\n\n1.2. REMOVALS AND CANCELLATIONS
\n\n2. STATEMENTS OF CONCERN
\n\nA Statement of Concern detailing alleged misconduct will be issued by the Academic Editor or publisher following a 3rd party report of scientific misconduct when:
\n\nIntechOpen believes that the number of occasions on which a Statement of Concern is issued will be very few in number. In all cases when such a decision has been taken by the Academic Editor the decision will be reviewed by another editor to whom the author can make representations.
\n\n3. CORRECTIONS
\n\nA Correction will be issued by the Academic Editor when:
\n\n3.1. ERRATUM
\n\nAn Erratum will be issued by the Academic Editor when it is determined that a mistake in a Chapter originates from the production process handled by the publisher.
\n\nA published Erratum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n3.2. CORRIGENDUM
\n\nA Corrigendum will be issued by the Academic Editor when it is determined that a mistake in a Chapter is a result of an Author’s miscalculation or oversight. A published Corrigendum will adhere to the Retraction Notice publishing guidelines outlined above.
\n\n4. FINAL REMARKS
\n\nIntechOpen wishes to emphasize that the final decision on whether a Retraction, Statement of Concern, or a Correction will be issued rests with the Academic Editor. The publisher is obliged to act upon any reports of scientific misconduct in its publications and to make a reasonable effort to facilitate any subsequent investigation of such claims.
\n\nIn the case of Retraction or removal of the Work, the publisher will be under no obligation to refund the APC.
\n\nThe general principles set out above apply to Retractions and Corrections issued in all IntechOpen publications.
\n\nAny suggestions or comments on this Policy are welcome and may be sent to permissions@intechopen.com.
\n\nPolicy last updated: 2017-09-11
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