The most common kinds of light sources.
\r\n\tDiagnostic tools are advancing: micro-and nano-diagnostics, advanced molecular genetics, and diagnosis of the aberrant clotting factor synthesis development and the options for the staging of the genetic abnormality - severe, moderate, and mild expression.
\r\n\tTreatment developments and advances start with prevention, intra-uterine approaches, genetic manipulation, genetic engineering, the high specificity of replacement factors, and recombinant technology.
\r\n\tIn addition to the above, the book will provide an update on the prevention of transmission of pathogens and potentially toxic substances used to stabilize and preserve treatment commodities. The role of big data and artificial intelligence through both machine learning and the application of deep learning and digital footprinting will also be addressed.
\r\n\tIn the developing world, there is an urgent need to collect, preserve and process plasma for the manufacturing of high yield, safe, and stabilized cryoprecipitate, or pharmaceutical fractionation of purified and specific clotting factors, as well as improvement on diagnostic and sociomedical approaches with an emphasis on patient and family care, and management of bleeding episodes.
Spectroscopy is the study of interactions between matter and different forms of electromagnetic radiation; when practiced to quantitative analysis, the term spectrometry is used.
Atomic spectroscopy includes the techniques of atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), atomic fluorescence spectroscopy (AFS), X-ray fluorescence (XRF), and inorganic mass spectroscopy (MS). AAS, AES, and AFS exploit interactions between UV-visible light and the valence electrons of free gaseous atoms. In XRF, high-energy charged particles collide with inner-shell electrons of atom, initiating transitions with eventual emission of X-ray photons. For inorganic MS, ionized analyte atoms are separated in a magnetic field according to their mass to charge (m/z) ratio [1].
Every element has a characteristic atomic structure, with a small, positively charged nucleus surrounded by a sufficient number of electrons necessary to maintain neutrality. Electrons settle into orbitals within an atom and one of the electrons can also jump from one energy level to the higher level by acquiring the necessitated energy (Figure 1). This energy is provided by colliding with other atoms, such as heating-AES, photons derived from light-AAS and AFS, or high-energy electrons-XRF. Possible transitions happen, when the required energy reaches to the difference between two energy states (ΔE). A neutral atom may exist at a low energy shell or ground state (E0), or at any of a group of excited states depending on how many electrons have been jumped to higher energy levels (E′’) although it is normal to think for the first transition. Each element has a unique energy level and the ΔEs associated with transitions between those levels.
Energy level diagrams to show transitions associated with (a) AAS, (b) AES, and (c) AFS. The vertical arrows indicate absorption or emission of light.
The ΔE for movements of
where h is Planck’s constant (6.63 × 10−34 Js) and υ the frequency of the waveform corresponding to that photon. The relationship between wavelength and frequency is showed by the equation below:
where c is the speed of light and
and a specific transition, ΔE, is associated with a unique wavelength.
When light of a specific wavelength enters an analytical system, outer shell electrons of the corresponding atoms will be excited as energy is absorbed. As a result, the amount of light transmitted from the system to detector will be reduced, this is understood as AAS (Figure 1a).
Under appropriate circumstances, outer shell electrons of vaporized atoms may be excited by heating. As these electrons return to the more stable ground state, energy is lost. As Figure 1b shows, some of this energy is emitted as light, which can be measured with a detector, this is AES.
Some of the radiant energy absorbed by ground state atoms can be emitted as light as the atom returns to the ground state i.e. AFS (Figure 1c).
When high-energy photons strike to a massive particle, it can excite an inner shell electron of the atom. The forming inner orbital vacancy can be filled with an outer shell electron. The transition is created by an emission of an X-Ray photon. This process is
The energy of the emission i.e. the wavelength is characteristic of the atom (element) from which it originated while the intensity of the emission is related to concentration of the atoms in the sample [7].
The high temperature inductively coupled plasma has been successfully used as an effective ion source for a mass spectroscopy, the type of method of inductively coupled plasma-mass spectroscopy (ICP-MS) is routinely used for measurements of trace elements in clinical and biological samples [8, 9].
It follows from Eqs. 1–3 that the wavelengths of the absorbed or emitted light are unique to a given element.
Formation of the atomic vapor i.e. atomization is the major principle of emission, absorption, and fluorescence techniques. The most critical component of instruments used in atomic spectroscopy is the atomization sources and sample introduction devices with an associated spectrometer for wavelength selection and detection of light. Atomization involves the several key (the basic) steps: solvent removal, separation from anion and other elements of the matrix, and reduction of ions to the ground state atom. The design of an AFS instrument is similar to those for AAS and AES except that the light source and the detector are located at a right angle (Figure 2).
Schematic diagram of an AAS, AES, and AFS instrument.
The most common kinds of light sources.
An ideal
Schematic diagram of wavelength selectors: (a) filters and (b) a diffraction grating monochromator.
where
Phototubes and photomultipliers include a photosensitive surface that absorbs radiation in the
Diagram of a phototube and a photomultiplier tube.
A transducer’s electrical signal is sent to a
Examples of detectors for spectroscopy.
An ideal sample preparation should remove interfering components from the matrix and to adjust of analyte to facilitate the actual measurement. Methods for destruction of the organic matrix by simple heating or by acid digestion have been developed and are thoroughly approved. Microwave heating is used for this purpose, with the specifically designed a compatible
Liquid-liquid portioning has been widely applied for preconcentration procedure. Analyte atoms in a large volume of aqueous solution are complexed with a suitable agent and collected into a small volume of solvent. Vapor generation procedures permit the rapid introduction of 100% of the sample into the atomizer and are used for AAS, AES, AFS, and ICP-MS. Certain elements such as arsenic, selenium, and bismuth readily evolve gaseous hydrides and transferred by a flow of inert gas to an AES, and ICP-MS or to a heated silica tube positioned in the light path for AAS, AFS. The tube can be heated using the air-acetylene flame or an electric current. The obtained heat is enough to cause decomposition of the hydride and atomization of the analyte. Thus, there is no loss off analyte, which in all the atoms flow the light path with in few seconds and they are trapped within the silica tube that was retarded their dispersion. Any sample volume added to the reaction container, hydride generation AAS has detection limits a few nanograms of analyte. Mercury can quickly form a vapor in the ambient temperature, and this property is the basis for cold vapor generation. When a reducing agent is added to sample solution, Hg2+ converts to the elemental mercury. Agitation or bubbling of gas through the solution is used to enhance rapid vaporization of the atomic mercury and to improve the transfer of mercury to a flow through cell located in the light path. As with hydride generation, the detection limit is a few nanogram and some manufacturers have been developed common instrumentation to accomplish both procedures. Chromatographic or electrophoretic techniques have been also developed that are coupled directly to the atomic spectroscopic instrument to develop integrated analytical arrangements [13].
The detection limits are important parameters of analytical techniques. Typical detection limit ranges for the major atomic spectroscopy techniques are shown in Figure 5. AAS detection limits are generally better in all cases where the element can be atomized. Detection limits for refractory elements such as bor, titanium, and vanadium are better by ICP than by AAS. Nonmetals and the halogens can only be determined by ICP. Optimum detection of nonmetals such as sulfur, nitrogen, and halogens by ICP-ES can only be achieved when a vacuum monochromator is used. For mercury and those elements that form hydrides, the cold vapor mercury or hydride generation techniques offer exceptional detection limits [14].
Typical detection limit ranges for the major atomic spectroscopy techniques.
The analytical working range can be considered as the concentration range over which quantitative results can be obtained without recalibration for system. Selecting a technique with an analytical working range based on the expected analyte concentrations, minimizes the analysis times by allowing the samples with different analyte concentrations to be analyzed together. For example; ICP-MS, once considered only an ultratrace element technique, can now run concentration ranges from low parts-per-trillion (ppt) level up to high parts per million (ppm). A wide analytical working range also can reduce, for example handling requirements, minimizing potential errors. Figure 6 shows typical analytical working ranges with a single set of instrumental conditions [15].
Analytical working ranges for the major atomic spectroscopy techniques.
Technique | Type of interference | Method of compensation |
---|---|---|
Flame AA | lonization Chemical Physical | lonization buffer Releasing agent or nitrous oxide-acetylene flame Dilution, matrix matching, or method of additions |
GFAA | Physical and chemical Molecular absorption Spectral | STPF conditions Zeeman or continuum source background correction Zeeman background correction |
ICP emission | Spectral Matrix | Background correction or the use of alternate analytical lines Internal standardization |
ICP-MS | Mass overlap Matrix | Inter element correction, use of alternate mass values, or higher mass resolution Internal standardization |
Atomic spectroscopy interferences.
Performance criteria for analytical techniques include the ease of use, required operator skills, and availability of documented methodology. Table 4 summarizes comparative advantages and limitations of the most common atomic spectroscopy techniques.
Criteria | Flame AA | Flame AE | AFS | ICP | X-RF |
---|---|---|---|---|---|
Costs | Low (~$10–15 K) | Moderate | Moderate | Highest (~$200 K) | Highest |
Instrumental ion | Low | Low | High | High | High |
Maintenance | Low | Low | Low | High | High |
Sample preparation | Moderate | Low | Moderate | High | |
Speed | Slow | Medium | Fast | Fast | |
Operator skill | Lower | Moderate | Higher | Higher | Higher |
Comparison of spectroscopic techniques performance.
Analytical methods of atomic spectroscopy have been used for elemental analysis identification, and quantitation in varieties of samples. Recently, most all of the spectroscopic techniques available are used in the analysis of metals and trace elements in samples of industrial and environmental origin.
Progress continues to develop in analytical spectroscopy as improvements are made to sensitivity, limits of detection, and availability. Recent development depends on instrumental adjustments and slight modifications to allow new types of measurements. Advancements in materials science have revealed demand for new methods of measurement using instruments already accessible, pushing the boundaries of what was previously available. For example, some new and interesting miniaturized plasma sources and a new distance of flight (DOF) mass spectrometer have been to the fore in developments. In addition, several novel methods have been developed, such as laser ablation molecular isotopic spectrometry (LAMIS) for isotope ratio analysis, and stand-off LIBS techniques such as “underwater LIBS” [16].
This chapter summarizes the key principles and application areas of atomic spectroscopy techniques. For example, a medical laboratory could determine the type and amount of heavy metals that could be present in patient’s serum or urine. Environmental scientists could monitor heavy metal contamination of water and soil. The pharmaceutical industry uses these techniques to determine metals and metalloids in drug products [17, 18].
Important criteria for selecting an analytical technique include detection limits, analytical working range, sample preparation, cost, ease of use, and the availability of proven methodology. Atomic spectroscopy techniques have provided a rapid, simple, accurate, and highly sensitive means of determining the concentrations of the elements.
In the future, it seems more likely that
In recent years, the emphasis of science and technology has shifted more toward environmental benign and sustainable resources and progress. Green Chemistry is paramount concept in chemistry for sustainability, which is the implementation of a set of principles that minimize or get rid of the utilization or generation of hazardous substances in the design, manufacture, and applications of chemical products [1]. Presently, Sonochemistry is a simplistic pathway for a huge variety of syntheses in organic chemistry. Hence, significant features of the ultrasound approach compared with traditional methods are in higher yields, milder conditions, lesser reaction times, improved reaction rates, formation of purer products, easier manipulation and a role in waste minimization and energy protection [2, 3, 4, 5].
Multicomponent reactions [6] leading to facinating heterocyclic scaffolds must appear as Potent tools for delivering the molecular diversity required in combinatorial approaches for the synthesis of bioactive compounds and producing varied chemical libraries of drug-like molecules for biological screening [7, 8]. Chalcones, or 1,3-diphenyl-2-propen-1-ones, are commonly occurring heterocyclic ring systems and are important structural motifs found in many natural products and pharmaceuticals. It is also known as benzalacetophenone and benzylidene acetophenone. Chalcones are one of the most important classes of flavonoids [9, 10]. Further ring closure reactions of Chalcones can be used to obtain various heterocyclic rings viz.; Pyrazoles, Pyrans, Cyanopyridines, isoxazoles and pyrimidines having different hetero-cyclic ring systems and multiple derivatives can be synthesized using chalcones [11, 12, 13, 14, 15].
The increased environmental concerns needed the replacement of present methods with new more sustainable processes which used the ionic liquids in place of organic catalysts and solvents [16, 17, 18, 19, 20, 21, 22, 23, 24, 25]. Ionic Liquids (ILs), as a class of molten salts, are composed entirely of ions and their melting point is around or below 100°C [26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36]. Due to short reaction times, mild reaction conditions, better yields, easy recyclability thermally stable, non-flammable character with negligible vapor pressure, adjustable miscibility with organic substrates and tunable solvating ability ionic liquids (ILs) have attracted the attention of organic chemists [37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49]. Furthermore, unique physiochemical properties that make them potential candidates for many applications in pharmaceuticals, industry and academia [50, 51, 52].
There are several varieties of ionic liquids being studied, out of them a few Simple functionalized ILs have created unparalleled fascination as they display some benefits for certain base-catalyzed processes, like easy recycling and better catalytic performance [53]. The environmentally benign basic ionic liquids are used as reaction media as well as catalysts in the development of multicomponent reactions (MCRs). Among all such basic ionic ILs [DBU][OAc] has shown the desired results. Some of the key benefits that can be highlighted for utilization of this IL as catalyst are, the desired product obtained without any further purification and the recyclability of the catalyst was found to be up to 5 cycles. The investigation of alternatives with the help of ionic liquids to conventional organic solvents is a developing research area due to increased environmental concerns.
Herein, we are especially interested in developing the potential use of efficient, simple methodology for the ring closure reactions of chalcones using [DBU][OAc] as ionic liquids as a solvent and catalyst. Chalcones can be used to obtain various heterocyclic rings through ring closure reactions (Figure 1).
General scheme of ring closure reactions of chalcones.
Melting points were recorded in open glass capillary tube using Gallenkamp melting point apparatus and are uncorrected. Checked by Thin layer chromatography (TLC) was applied to check the purity of synthesized compounds and Spots were visualized by irradiation with UV lights (254 nm) or by staining with iodine vapors. The Fourier-transform infrared (FT-IR) spectra were recorded on SHIMADZU 8400S FT-IR spectrophotometer and wave number is given in cm−1. The 1H NMR spectra and 13C NMR (by broad band proton decoupling technique) were recorded on JEOL AL spectrometer in CDCl3/DMSO-d6 solvents at 400 and 100 MHz and chemical shift were measured in δ ppm relative to TMS as an internal standard. The Mass (HRMS) spectra were recorded on JEOL SX 102/DA-600 using Argon/Xenon gas. The elemental analysis (C, H and N) were performed using vario-III analyzer at CDRI Lucknow.
According to the reported literature [DBUH][OAc] ILs [54] and [DBUH][Cl] ILs [55] were synthesized by the reaction of 2,3,4,6,7,8,9,10-octahydropyrimido[1,2-a]azepine (DBU) and acetic acid or hydrochloric acid, respectively.
Chalcones were synthesized according to the reported procedure with minor modification (Figure 2), the synthesized products were characterized by 1H NMR, and physical data and compared with those reported in literature [54].
General procedure of synthesis of chalcones (3a-c).
Chalcone derivative (1 mmol) and methylhydrazine (1 mmol) were ultrasonicated catalyzed by [DBUH][OAc] (5 ml) at 50°C for about 4 h (Figure 3). The crude product was refrigerated overnight. The precipitate formed was filtered off and crystallized from ethanol yielding yellow crystals of the product (4a).
Model reaction for preparation of pyrazole derivative (4a).
1H NMR (400 MHz, DMSO-d6) δ 8.81, 7.94, 7.59, 7.47, 7.44, 7.38, 3.96; 13C NMR (100.15 MHz, DMSO-d6) δ 145.51, 133.32, 131.99, 130.37, 129.66, 128.69, 128.34, 126.78, 125.67, 123.12, 122.13, 40.57; HRMS;
Chalcone derivative (1 mmol) mixed with
Model reaction for preparation of pyran derivative (5a).
1H NMR (400 MHz, DMSO-d6) δ 7.45, 7.32, 7.28, 7.25, 7.01, 5.27, 3.60, 2.28, 2.21, 2.12, 2.05; 13C NMR (100.15 MHz, DMSO-d6) δ 195.02, 164.92, 142.45, 138.87, 131.92, 129.14, 128.97, 128.27, 128.12, 122.84, 107.13, 76.87, 35.62, 35.14, 27.61, 16.76; HRMS;
A mixture of chalcone derivative (2 mmol) with malononitrile (2 mmol) in 5 mL of [DBUH][OAc] was ultrasonicated at atmospheric pressure at 65°C for 3 h (Figure 5). After completion of the reaction, the mixture was cooled to room temperature and the organic layer was concentrated. The pure product was obtained by column chromatography (n-hexane:ethyl acetate = 80:20) to afford the preferred product (6a).
Model reaction for preparation of cyanopyridine derivative (6a).
1H NMR (400 MHz, DMSO-d6) δ 9.21, 8.40, 7.95, 7.62, 7.51, 7.44, 7.46; 13C NMR (100.15 MHz, DMSO-d6) δ 160.21, 152.79, 151.01, 138.51, 138.45, 131.34, 130.03, 129.67, 128.99, 127.91, 121.91, 120.71, 117.22, 110.19; HRMS;
Chalcone derivative (1 mmol) was ultrasonicated with hydroxylamine hydrochloride (1 mmol) in catalytic influence of [DBUH][OAc] ILs (5 mL) at 70°C for 1 h (Figure 6). The formation of product was monitored by TLC. Isoxazole derivative was obtained by keeping the reaction mixture on ice bath, then the desired product was isolated, washed with water, and dried (7a).
Model reaction for preparation of isoxazole derivative (7a).
1H NMR (400 MHz, DMSO-d6) δ 8.66, 7.69, 7.57, 7.50, 7.41, 7.32; 13C NMR (100.15 MHz, DMSO-d6) δ 170.26, 154.95, 131.82, 130.67, 128.61, 128.30, 128.07, 127.55, 126.73, 125.41, 116.74; HRMS;
To the mixture of chalcone derivative (1 mmol), guanidine hydrochloride (2 mmol) was added with [DBUH][OAc] ILs was heated under ultrasonication for 2 h at 55°C. The completion of the reaction was checked by TLC (Figure 7). The reaction mixture poured into ice water and formed product was filtered and recrystallized from ethanol (8a).
Model reaction for preparation of pyrimidine derivative (8a).
1H NMR (400 MHz, DMSO) δ 7.77, 7.64, 7.47, 7.41, 7.15, 2.29; 13C NMR (100.15 MHz, DMSO-d6) δ 160.02, 158.70, 138.09, 136.17, 132.01, 131.05, 130.28, 129.20, 128.35, 125.47, 112.89; HRMS;
General representation of preparation of chalcone derivatives (4-8a-c).
In this chapter, the ring closure reaction of chalcone derivatives in the presence of basic ionic liquid [DBUH]OAc to afford the several derivatives like pyrazoles, pyrans, pyrimidines, isoxazoles, and cyanopyridines. Different catalytic systems were used to optimize the reaction conditions on the set of model reactions.
The reaction conditions were optimized on the respective model reactions, further these optimized reaction conditions were used to produce corresponding derivatives of chalcones (Table 1).
% yield of desired products (4-8a,b,c).
We have carried out the synthesis of a number of chalcone derivatives (4-8a,b,c) under different reaction conditions. The optimized conditions for all the ring closure reactions of chalcones involved use of [DBUH]OAc ILs as catalyst under sonication for appropriate time at adequate temperature (Table 2, Entry 6).
S. No. | Catalyst / Solvent | Reaction Condition | Pyrazole Derivative (4a) | Pyran Derivative (5a) | Cyanopyridine Derivative (6a) | Isoxazole Derivative (7a) | Pyrimidine Derivative (8a) | |||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Temp (°C) | % Yield | Temp (°C) | % Yield | Temp (°C) | % Yield | Temp (°C) | % Yield | Temp (°C) | % Yield | |||
1. | No catalyst / DCM | Reflux | 120 | <5 | 120 | <5 | 120 | <5 | 120 | <5 | 120 | <5 |
2. | NaOH / DCM | Sonication | 100 | >15 | 100 | >15 | 100 | >15 | 100 | >15 | 100 | >15 |
3. | [MIM]BF4 IL | Sonication | 100 | 40 | 100 | 35 | 100 | 42 | 100 | 45 | 100 | 38 |
4. | [MIM]OH IL | Sonication | 90 | 48 | 90 | 42 | 90 | 51 | 90 | 55 | 90 | 44 |
5. | [DBUH]Cl IL | Sonication | 90 | 62 | 90 | 65 | 90 | 68 | 90 | 62 | 90 | 60 |
6. | [DBUH]OAc IL | Sonication | 50 | 97 | 60 | 95 | 65 | 93 | 70 | 94 | 55 | 96 |
Optimization of reaction conditions.
The catalytic reusability of ILs was observed during optimized reaction conditions. The ILs were easily recovered as filtration after the completion of reaction. The recovered ILs were used four times without remarkable loss in activity but after that there is sudden decrease (Figure 9) in yield of products.
Reusability and recyclability of [DBUH]OAc ILs.
In Summary, we developed a simple and efficient catalytic system that can effectively promote the conversion of chalcones into different derivatives viz.; Pyrazoles, Pyrans, Cyanopyridines, isoxazoles and pyrimidines
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\n\nCSIC affiliated authors can also take advantage of a central Open Access fund (amounting to 10,000 EUR) to cover up to 50% of the rest of the OAPF until it expires. Effective for chapters accepted from January 1, 2020.
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\n\nCorresponding authors will receive a 25% discount on their Open Access Publication Fees (OAPF) for Open Access book chapters. A 20% discount for publishing a long-form monographs, 25% for compacts and 23% for short-form monographs.
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Hatch",authors:[{id:"130802",title:"Dr.",name:"Grant",middleName:null,surname:"Hatch",slug:"grant-hatch",fullName:"Grant Hatch"},{id:"170126",title:"Prof.",name:"Donald",middleName:null,surname:"Miller",slug:"donald-miller",fullName:"Donald Miller"},{id:"170127",title:"Mr.",name:"Michael",middleName:null,surname:"Pogorzelec",slug:"michael-pogorzelec",fullName:"Michael Pogorzelec"},{id:"170128",title:"Ms.",name:"Hieu",middleName:null,surname:"Nguyen",slug:"hieu-nguyen",fullName:"Hieu Nguyen"},{id:"170129",title:"Dr.",name:"Ngoc",middleName:null,surname:"On",slug:"ngoc-on",fullName:"Ngoc On"},{id:"170130",title:"Dr.",name:"Siddhartha",middleName:null,surname:"Dalvi",slug:"siddhartha-dalvi",fullName:"Siddhartha Dalvi"}]},{id:"62431",doi:"10.5772/intechopen.79266",title:"The United Chemicals of Cannabis: Beneficial Effects of Cannabis Phytochemicals on the Brain and Cognition",slug:"the-united-chemicals-of-cannabis-beneficial-effects-of-cannabis-phytochemicals-on-the-brain-and-cogn",totalDownloads:1841,totalCrossrefCites:4,totalDimensionsCites:12,abstract:"‘Medicinal cannabis’ can be defined as pharmaceutical grade cannabis-based products used for the treatment of illness. Beneficial treatment effects of cannabidiol (CBD), a major non-intoxicating compound isolated from the cannabis plant, have been shown in multiple states of cognitive impairment, including neurodegenerative (Alzheimer’s, Huntington’s and Parkinson’s disease), neuroinflammatory (sepsis-induced encephalopathy) and neurological disorders (ischemic brain injury). CBD can also treat some of the symptoms of schizophrenia, including cognitive deficits (impairments in learning and memory), which is a major symptom domain of the illness that is largely resistant to existing antipsychotic medications. However, empirical evidence suggests the presence of an ‘entourage effect’ in cannabis; that is, observations that medicinal cannabis seems to work better in some instances when administered as a whole-plant extract. While scientific evidence highlights isolated CBD as a strong candidate for treating cognitive impairment, the entourage effect suggests that the co-operation of other plant molecules could provide further benefits. This chapter explores the scientific evidence surrounding the benefits of CBD and other specific key phytochemicals in cannabis: linalool, α-pinene, β-caryophyllene, flavonoids and anthocyanin, on brain health and cognition.",book:{id:"7040",slug:"recent-advances-in-cannabinoid-research",title:"Recent Advances in Cannabinoid Research",fullTitle:"Recent Advances in Cannabinoid Research"},signatures:"Katrina Weston-Green",authors:null},{id:"64031",doi:"10.5772/intechopen.81224",title:"Trends of Protein Aggregation in Neurodegenerative Diseases",slug:"trends-of-protein-aggregation-in-neurodegenerative-diseases",totalDownloads:1622,totalCrossrefCites:6,totalDimensionsCites:11,abstract:"Protein aggregation trends in neurodegenerative diseases are largely unmapped due to the complex nature of protein-protein interactions and their regulatory machineries such as protein proteolytic systems. Since the protein aggregation process in humans is a slow process, early determination of the patients that will develop neurodegenerative diseases later in life is critical in terms of starting effective treatment, which will reduce the expensive health care. In this chapter, I will discuss the nature of protein aggregation of signature proteins and the status of protein proteolytic systems such as proteasome and autophagosome in Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, frontotemporal lobar degeneration, Huntington’s disease, and prion disease under the light of recent studies including our new findings.",book:{id:"7480",slug:"neurochemical-basis-of-brain-function-and-dysfunction",title:"Neurochemical Basis of Brain Function and Dysfunction",fullTitle:"Neurochemical Basis of Brain Function and Dysfunction"},signatures:"Abdulbaki Agbas",authors:[{id:"250609",title:"Prof.",name:"Abdulbaki",middleName:null,surname:"Agbas",slug:"abdulbaki-agbas",fullName:"Abdulbaki Agbas"}]}],mostDownloadedChaptersLast30Days:[{id:"57103",title:"GABA and Glutamate: Their Transmitter Role in the CNS and Pancreatic Islets",slug:"gaba-and-glutamate-their-transmitter-role-in-the-cns-and-pancreatic-islets",totalDownloads:3565,totalCrossrefCites:4,totalDimensionsCites:10,abstract:"Glutamate and gamma-aminobutyric acid (GABA) are the major neurotransmitters in the mammalian brain. Inhibitory GABA and excitatory glutamate work together to control many processes, including the brain’s overall level of excitation. The contributions of GABA and glutamate in extra-neuronal signaling are by far less widely recognized. In this chapter, we first discuss the role of both neurotransmitters during development, emphasizing the importance of the shift from excitatory to inhibitory GABAergic neurotransmission. The second part summarizes the biosynthesis and role of GABA and glutamate in neurotransmission in the mature brain, and major neurological disorders associated with glutamate and GABA receptors and GABA release mechanisms. The final part focuses on extra-neuronal glutamatergic and GABAergic signaling in pancreatic islets of Langerhans, and possible associations with type 1 diabetes mellitus.",book:{id:"6237",slug:"gaba-and-glutamate-new-developments-in-neurotransmission-research",title:"GABA And Glutamate",fullTitle:"GABA And Glutamate - New Developments In Neurotransmission Research"},signatures:"Christiane S. Hampe, Hiroshi Mitoma and Mario Manto",authors:[{id:"210220",title:"Prof.",name:"Christiane",middleName:null,surname:"Hampe",slug:"christiane-hampe",fullName:"Christiane Hampe"},{id:"210485",title:"Prof.",name:"Mario",middleName:null,surname:"Manto",slug:"mario-manto",fullName:"Mario Manto"},{id:"210486",title:"Prof.",name:"Hiroshi",middleName:null,surname:"Mitoma",slug:"hiroshi-mitoma",fullName:"Hiroshi Mitoma"}]},{id:"58817",title:"Clinical Application of MR Spectroscopy in Identifying Biochemical Composition of the Intracranial Pathologies",slug:"clinical-application-of-mr-spectroscopy-in-identifying-biochemical-composition-of-the-intracranial-p",totalDownloads:2097,totalCrossrefCites:0,totalDimensionsCites:5,abstract:"Magnetic resonance spectroscopy (MRS) provides useful information regarding metabolic composition in the tissues, and advanced spectroscopic methods are used to quantify markers of tumor membrane turnover and proliferation (e.g., choline (Cho)), energy homoeostasis (e.g., creatine (Cr)), intact glioneuronal structures (e.g., N-acetylaspartate (NAA)), and necrosis (e.g., lactate (Lac) or lipids). Results are usually expressed as metabolite ratios rather than absolute metabolite concentrations. Because glial tumors have some specific metabolic characteristics that differ according to the grade of tumor, there is a potential for MR spectroscopy to increase the sensitivity of routinely used diagnostic imaging. MRS also has many diagnostic applications in neurosciences to support the diagnosis in conditions like demyelination, infections, and dementia and in postradiotherapy cases. Biochemical changes in the metabolism of tumor cells related to malignant transformation are reflected in changes of particular metabolite concentration in the tumor tissue. Our prospective study aimed to analyze the usefulness of proton MR spectroscopy in grading of glioma and to correlate various metabolite ratios like choline/creatine, choline/N-acetylaspartate, N-acetylaspartate/creatine, and lactate/creatine with the histopathological grades of glioma.",book:{id:"6237",slug:"gaba-and-glutamate-new-developments-in-neurotransmission-research",title:"GABA And Glutamate",fullTitle:"GABA And Glutamate - New Developments In Neurotransmission Research"},signatures:"B C Hamsini, Bhavana Nagabhushana Reddy, Sankar Neelakantan\nand Sunitha Palasamudram Kumaran",authors:[{id:"211054",title:"Dr.",name:"Sunitha",middleName:null,surname:"P Kumaran",slug:"sunitha-p-kumaran",fullName:"Sunitha P Kumaran"},{id:"221485",title:"Dr.",name:"Sankar",middleName:null,surname:"Neelakantan",slug:"sankar-neelakantan",fullName:"Sankar Neelakantan"},{id:"398223",title:"Dr.",name:"B C",middleName:null,surname:"Hamsini",slug:"b-c-hamsini",fullName:"B C Hamsini"},{id:"398224",title:"Dr.",name:"Bhavana",middleName:null,surname:"Nagabhushana Reddy",slug:"bhavana-nagabhushana-reddy",fullName:"Bhavana Nagabhushana Reddy"}]},{id:"62431",title:"The United Chemicals of Cannabis: Beneficial Effects of Cannabis Phytochemicals on the Brain and Cognition",slug:"the-united-chemicals-of-cannabis-beneficial-effects-of-cannabis-phytochemicals-on-the-brain-and-cogn",totalDownloads:1845,totalCrossrefCites:4,totalDimensionsCites:12,abstract:"‘Medicinal cannabis’ can be defined as pharmaceutical grade cannabis-based products used for the treatment of illness. 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This chapter explores the scientific evidence surrounding the benefits of CBD and other specific key phytochemicals in cannabis: linalool, α-pinene, β-caryophyllene, flavonoids and anthocyanin, on brain health and cognition.",book:{id:"7040",slug:"recent-advances-in-cannabinoid-research",title:"Recent Advances in Cannabinoid Research",fullTitle:"Recent Advances in Cannabinoid Research"},signatures:"Katrina Weston-Green",authors:null},{id:"68776",title:"Introductory Chapter: The Chemical Basis of Neural Function and Dysfunction",slug:"introductory-chapter-the-chemical-basis-of-neural-function-and-dysfunction",totalDownloads:1133,totalCrossrefCites:1,totalDimensionsCites:2,abstract:null,book:{id:"7480",slug:"neurochemical-basis-of-brain-function-and-dysfunction",title:"Neurochemical Basis of Brain Function and Dysfunction",fullTitle:"Neurochemical Basis of Brain Function and Dysfunction"},signatures:"Thomas Heinbockel and Antonei B. Csoka",authors:[{id:"70569",title:"Dr.",name:"Thomas",middleName:null,surname:"Heinbockel",slug:"thomas-heinbockel",fullName:"Thomas Heinbockel"},{id:"245650",title:"Dr.",name:"Antonei B.",middleName:null,surname:"Csoka",slug:"antonei-b.-csoka",fullName:"Antonei B. Csoka"}]},{id:"68712",title:"Synaptic Transmission and Amino Acid Neurotransmitters",slug:"synaptic-transmission-and-amino-acid-neurotransmitters",totalDownloads:1383,totalCrossrefCites:6,totalDimensionsCites:7,abstract:"Amino acids are the most abundant neurotransmitters in the brain. Neurotransmitters are synthesized and stored in presynaptic terminals, released from terminals upon stimulation with specific receptors on the postsynaptic cells. Chemical and electrical synapses are specialized biological structures found in the nervous system; they connect neurons together and transmit signals across the neurons. The process of synaptic transmission generates or inhibits electrical impulses in a network of neurons for the processing of information. Glutamate is the primary excitatory neurotransmitter in the brain, while GABA is the principal inhibitory neurotransmitter. The balance of glutamatergic and GABAergic tone is crucial to normal neurologic function. Through synaptic transmission, this information is communicated from the presynaptic cell to the postsynaptic cell. Amino acid neurotransmitters primarily glutamic acid, GABA, aspartic acid, and glycine are single amino acid residues released from presynaptic nerve terminals in response to an action potential and cross the synaptic cleft to bind with specific receptor on the postsynaptic membrane. The integral role of amino acid neurotransmitters is important on the normal functioning of the brain. The presynaptic and postsynaptic events in chemical synapses are subject to use dependent and highly regulated as per the changes in synaptic neurotransmitter release and function.",book:{id:"7480",slug:"neurochemical-basis-of-brain-function-and-dysfunction",title:"Neurochemical Basis of Brain Function and Dysfunction",fullTitle:"Neurochemical Basis of Brain Function and Dysfunction"},signatures:"Manorama Patri",authors:[{id:"196763",title:"Dr.",name:"Manorama",middleName:null,surname:"Patri",slug:"manorama-patri",fullName:"Manorama Patri"}]}],onlineFirstChaptersFilter:{topicId:"212",limit:6,offset:0},onlineFirstChaptersCollection:[],onlineFirstChaptersTotal:0},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:90,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:107,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:330,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:9,numberOfPublishedChapters:139,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:122,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:112,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:21,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:10,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. 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After that, he was a postdoc research fellow at the University of British Columbia in Canada to do research on large-scale stream experimental manipulation and watershed ecological survey in temperate rainforests of BC. He was a faculty member at the University of Hong Kong to run ecological research projects on aquatic insects, fishes, and newts in Tropical Asian streams. He also conducted research in streams, rivers, and caves in Texas, USA, to study the ecology of macroinvertebrates, big-claw river shrimp, fish, turtles, and bats. 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