Collagen based biomaterials for 3D printed tissues.
\r\n\tHealth and mental health issues of both children and adults and evidence-based treatments will be included. The types of sexual violence that occur and prevention efforts that have – or have not – been made to address the occurrence of these types of violence will be covered.
\r\n\r\n\tCultural and governmental policies, as well as legal and jurisdiction issues to address victims of these crimes, will also be incorporated in the book. For instance, Meagan’s Law and its worthiness in protecting children will be incorporated as well as the Federal program to reimburse victims of online child pornography and the legal entanglements and ramifications of that program.
\r\n\r\n\tThe typology of offenders and the effectiveness of treatment will also be addressed.
\r\n\tFinally, the direction of prevention strategies, treatment needs for both victims and offenders, and policy issues to move the field forward, particularly in terms of research, will be presented. The field of sexual violence has made significant strides in the past 45 years in terms of understanding sexually deviant behavior, the impact on children who then experience the symptoms of that trauma in adulthood, how to effectively interview sexually victimized children, and finally, treatment and social mores that make disclosure possible and recovery hopeful.
Conflict ravaging the Lake Chad Basin region (LCB), driven by the Boko Haram-led insurgency, has taken monstrous proportions. The direct impact of this reflects in the socioeconomic state of populations and countries in the environs. This chapter discusses the socioeconomic impact of the Boko Haram insurgency in the Lake Chad region. In analyzing the current situation, we will take into cognizance the historical antecedence of the region and the Boko Haram insurgency, as well as the group tactics and strategy in understanding the socioeconomic impact on the area. A microlevel analysis, studying violent extremism and the economic survival of people affected by the conflict, will be engaged. This is followed by the impact of this insurgency on the LCB and surrounding states at the macro-level.
\nOver time, the LCB has suffered various issues, from drought, severe effects of climate change, desertification, and poor governance that have contributed to deep poverty in the region. The LCB is encapsulating borders of Cameroon, Nigeria, and Chad. About 30 million people derived their means of livelihood from the resource provided by the 2,500,000 km2 lake in this semiarid area [1]. Precipitation in the lake annually reduces from the south (more than 1000 mm yr. − 1) to the north of the basin (less than 100 mm yr. − 1) [2]. The Chari/Logone River system may account for 90% of water inflow to the LCB, which has shrunk from 22,000 km2 to approximately 300 km2 between the 1960s and the 1980s [3]. This loss has been attributed to climate change and drought among other factors and has created a significant socioeconomic impact on the area [4].
\nThe violent extremist activities started in Borno State, northeast Nigeria, by Boko Haram which spread into adjacent Lake Chad and became a regional problem. In 2002, Boko Haram came to the limelight in Nigeria as an Islamist terrorist group of the Sunni strain for preaching Jihad. Its formal name in Arabic is Jamā’a Ahl al-sunnah li-da’wa wa al-jihād, which is translated from Arabic to mean, “people committed to the propagation of the Prophet’s teachings and Jihad” [6]. The phrase Boko Haram is, however, the widely accepted name of the group or more recently the Islamic State’s West Africa Province (ISWAP).
\nThe group has unleashed violence across the Lake Chad Basin region of West Africa, mostly in Nigeria, Niger, Chad, and Cameroon. It was founded by Muhammed Yusuf, but the mantle of leadership was taken by Abubakar Shekau, the current leader after Yusuf was killed [7]. Translated from Hausa, “Boko Haram” means “Western education is sinful,” and this reflects the group’s two main aims: the opposition of what it considers to be the secular westernization of Nigeria, especially coeducational learning and democratic elections, and the creation of an Islamic state in Nigeria or at least in the country’s majority-Muslim northern states [8].
\nThe crises are now raging along the borders of Cameroon, Chad, and Niger. Between 2014 and 2015, the group held full control over large areas in northeast Nigeria. Later in 2015, however, Nigeria and the other countries affected in coalition succeeded in taking back many of those territories captured by Boko Haram. The insurgents have morphed their tactics to a guerrilla war-type, using suicide attacks, especially with women as lethal weapons. Their refuge being in areas around the Lake Chad hills around the Nigeria-Cameroon border and forests in Borno State [5].
\nThe founder of the movement which became Boko Haram, Mohammed Yusuf, was heavily influenced by the ideology and writings of Ibn Taymiyyah, a Salafi scholar [9] explained some of Yusuf’s beliefs thus, “…quoting copiously from Ibn Taymiyyah, Mohammed Yusuf describes as taghut (idolatory) any form of executive, legislative or judicial function derived from a secular constitution rather than from Islamic Shariah Law.” This is at the root of his opposition to secularism, democracy, and partisan politics as practiced in Nigeria [8].
\nAfter the extrajudicial killing of Mohammed Yusuf by the Nigerian Army, Boko Haram became less visible in debate and discussion and greatly manifested in violence. The post-Mohammed Yusuf Boko Haram that evolved displayed a very violent agenda of political domination and establishment of a Sharia state. To attain this lofty goal, all elements of modern government reflective of western civilization had to be destroyed. All people unsympathetic to the sect’s ideology must also be exterminated. The
The group has modified its tactics through the years. Old methods like killing security officials, village heads, and security operatives changed to bombing infrastructure, market places, and kidnap [9]. Boko Haram pledged allegiance to ISIS in March 2015 and split into two factions. Abubakar Shekau continues to lead the Boko Haram faction and Umar Al barnawi, the IS-West Africa faction as they are known.
\nBoko Haram, like many terrorist groups, are not static but can alter their mission and approaches, to take on different shapes as they move from one location to the next—much as a sand dune does. They should be described and understood according to how they evolve and what their internal and external organizational patterns are, rather than what they happen to be at a given moment [10]. Instead of seeing these organizations as networks or hierarchical structures, it is described as a dune-like organization which shifts with the wind and is continually reshaped [11]. These key characteristics are evident in Boko Haram as well as Al-Qaeda, Hezbollah and Hamas which include adherence to a grand vision like a global jihad, no institutional presence, giving a ghost-like organizational reality based on its disappearance when necessary, and dynamic activity that sticks to sequential reasoning, among others.
\nUsing the association between networks’ density, centrality, and their outputs to evaluate the operational success of terror networks, [12] came up with conclusions that apply to Boko Haram today. He says the group’s success in operations and resilience in its ability to survive and to continue to generate a systematic campaign of violence or, more specifically, its durability and productivity describe its success [12]. The significant use of women from kidnapping to suicide bombing seems to show a pattern that reflects Faure and Zartman’s [10] description of stages in terrorist activities. This phase is described as the commercialization phase, using strategic violence, follows the incubation stage in the organizational life cycle of the group. For Boko Haram, this strategy would supposedly help deliver on their mission and goals. It would hold the attention of their target audience (government, public as well as the rest of the world).
\nBoko Haram has deployed different tactics in driving terrorist activities in the LCB region. In analyzing factors using quantitative tools that lead terrorist organizations to attack undefended civilians, which they called “soft targets,” Asal et al. examined two distinct processes in choosing to attack soft targets. The first one being the one-time decision to begin attacking soft targets and, second, being the continued use of violence against such targets. The analysis pointed to ideology—specifically religion—as the main factor in using civilians as targets, while organizational factors like group size and network centrality are related to the number of incidents perpetrated [13]. According to Bloom and Matfess [14], as Boko Haram shifted in its demography and prioritizing in terms of suicide operations, it adopted the “unexpected bomber” profile of women and children.
\nThe year 2013 showed an evolution in Boko Haram’s tactics. Boko Haram carried out kidnappings, in which one of the main characteristics was the instrumental use of women. From data and reports from Mendelboim and Schweitzer [15], in 2017, a record of 126 women and girls accounting for 92% of the global figures of female suicide bombers were of the Boko Haram sect, carrying out attacks in Africa. It has been argued, and logically so, that Boko Haram’s recognition by global media gained from the Chibok kidnappings in April 2014 helped establish this pattern [16, 17]. The #BringBackOurGirls campaigns, after the group abducted about 276 school-age girls from a school in Chibok, Northeastern Nigeria, caused an increase in the use of women in waging war. Understandably, Boko Haram realized the potency of gendered dimensions, especially in media for its campaign. This is evidenced in terms of its current strategy and notoriety and continued pattern. Looking at the trend of actions between 2002 when the group started in 2019, the use of women only picked up after the Chibok case. It is crucial to note the specific role the media played.
\nGenerally, inhabitants of the LBC live with relatively high poverty rate and population pressure, with about 50 people per km2 [19]. The human population in the area is expected to grow annually by an estimated 2.5–3.0% [18]. The area is also prone to intense water scarcity with access to less than 550 m3 of water per year [20]. Their challenges include poor medical facilities, human literacy, and inadequate water supply [21]. The critical factor to the economy of this region is the level of displacement resulting from the conflict and the consequent unemployment and deprivation. As Boko Haram continues its attacks in the area, thousands are forced to flee across the borders between the countries around the LCB, and within the states [22], records of thousands of refugees fleeing Nigeria to Cameroon when insurgents sacked Rann, one of the border towns, recorded. The same was the case with over 5000 inhabitants of Baga, another city on the border of Chad, fleeing across the Lake into Ngouboua village in Chad. This new demographic is dependent on aid and accounts for a swelling vulnerable population and fragile economy in the LCB.
\nThe main points for trade and access to local markets for inhabitants, which are the borders are mostly closed for security. Some livestock farmers move their herds through longer routes passing Niger, from Chad to sell at higher prices at the border markets. Commercial activities from fishing and agriculture have become restricted. Counter-terrorism endeavor by joint task force has been recorded to involve “clearing out” areas around the border, surrounding forests, and the lake shores. This military style operations targeted at flushing out insurgents have the side effect of destabilizing the lives of local fishermen and farmers [5]. Maiduguri, in Borno State, Nigeria, has an ancient history of serving not only as a cultural and religious hub but as the commercial heartbeat of the LCB region. Going back to thousands of years culturally, the Kanem-Bornu Empire and Kanembu of Chad are akin to the Kanuri in Nigeria in the way of life and language. The threat of constant Boko Haram activities over the last few years in the area has negatively affected business in the region and the rest of Northeastern Nigeria.
\nFollowing the high levels of displacement and refugee status, inhabitants of the LCB seeking a means of generating income and employment engage in hawking agric produce and menial tasks. Many send their children to Koranic schools, entrusting their children whom they are unable to care for to religious teachers. In many cases, this leads to radicalization of the children and the perpetuation of extremism [23]. Scholarly work on reasons why insurgency and terrorism may thrive shows that the socioeconomic environment is a critical predictor. The response of people, especially women, to this is participation in these organizations [24, 25]. Governance failure, corruption, economic marginalization leading to poverty, unemployment, inequality, and hunger are challenges faced by people in Nigeria and the LCB in varying degrees. According to Matfess [26], and corroborated by Walker [27], some Boko Haram women exercised agency in joining the ranks to make their quality of life better. Contrary to the argument of some scholars like Zedalis [28], regarding this point poverty does breed terrorism.
\nRadicalization is associated with poverty in some cases [29], in the context of Northeastern Nigeria and the LCB, radical groups offer economic prospects. A
The history and culture of an area influence the nature of insurgency in the area. The long history of drought, for example, had left the LCB and Northeast Nigeria in poverty, before the insurgency started. Colonization played a part in the history and origins of conflict in Nigeria. In Nigeria, the colonial masters—Britain—left a historical legacy of social fractures in the northeast, making it easier for Boko Haram to recruit followers in the 2000s. The colonialists handpicked the mountain people from Gwoza, made them the elite class and the larger group—the Dgwhede who felt marginalized and are currently some of the most militant Boko Haram fighters [27].
\nThe Kanuri-speaking people, covering Gwoza, Dgwhede, Borno, and Northeast Nigeria, also had a culture of
Consistent across findings from the previous study, development reports, and media analysis are the facts of a socioeconomic crisis in the LCB. Various policy recommendations have been made on improving the human and economic conditions of life in the LCB from academia to development experts. The World Bank, in building resilience for the area, initiated the Lake Chad Basin Commission (LCBC) to engage with the challenges and developed a plan. The purpose of the LCBC is to reduce poverty and address climate change and food security. Furthermore, the LCBC, in cooperation with the French Development Agency, affected countries, and the World Bank, plans on allocation of about $1 billion to this cause. The priority themes for allocating the funds, however, show a paltry 8% for managing conflicts in the area [33].
\nThe impact of Boko Haram activity in the area may require a direct or indirect significant allocation of resource in policy design, to defeat the insurgency, for meaningful progress to take place. Some have advocated for better cooperation between the countries in the LCB instead of closing borders, which increases poverty [5]. This approach may have a better potential for socioeconomic development in the region but has to take into account the large migrant flows between the countries as a result of the insurgency. The recommendation made by Okpara, Stringer, and Doughill [34] is vital to this end. They state that interventions should aid cohabitation as well as income opportunities for mixed migrants in the area.
\nMigrant populations displaced by the insurgency live as IDP’s in camps within the northeast of Nigeria. These people, in their millions, have lost access to farmlands, tools, and markets to create a livelihood for themselves or contribute to the local economy. Many have fled across the national borders into Chad and Cameroon depending on aid for food and shelter and putting further stress on scarce natural and economic resources. The existing background of deep lack and deprivation in the LCB accelerated by Boko Haram attacks calls for solutions proffered with security implications well considered. While the shrinking lake and harsh climate have left a hitherto agrarian region severely affected by drought and lack of water, the impact of conflict concludes the disaster scenario. This triad of devastation can be engaged at different levels; however, for socioeconomic improvement to proceed, peace has to return as a precursor to investment, employment, and trade.
\nProposed interventions with strategic components addressing security in the area directly as a step to socioeconomic change may be more impactful. In this regard, Angerbrandt [35] states the need for coordinating regional demobilization plans for vigilante members and stopping conflicts before they escalate. Enobi and Johnson-Rokosu [36] posit that eliminating terrorist finances or considerable reductions to their illicit financial flows by the government, and global effort is the direction to take. Providing water and food in critical parts of the LCB will remove an essential recruiting tool from Boko Haram. This is because 1.9 million displaced people and 2.7 million people with food insecurity live in the area according to the displacement tracking matrix of the IOM [37]. These people whose needs are as essential as food and water would succumb to offers that would offer these seeming benefits [38].
\nMeaningful intervention in the socioeconomic development of the LCB should not exclude significant investments in security, counter-terrorism measures, and food programs. Shelter, infrastructure, access to markets, and gender-informed policies are necessary to guide aid as well as policy and infrastructure that are gender-informed. The possibility of reviving trade in spite of the damage already done to the economy will have to entail more than military effort [5].
\nA clear strategy that considers the multilevel intersectionality of factors that drive the Boko Haram strategy and informs the economic condition in the area should be the basis of interventions. These factors are the unique historical, sociocultural, and development context of the Lake Chad Basin as well as the effect of climate change on the lake and livelihood. Policy implementation that will defeat the insurgency and direct investment into the region and boost socioeconomic development in the short- and long-term may not be easy to enact. This is because of the governance problems, corruption, and structural patriarchy that are common to the countries in the LCB. The gains, however, are essential and worth the effort—the equivalent of saving human lives.
\nIn making recommendations, an essential point to note is the question of responsibility. In whose hands does the buck of responsibility regarding socioeconomic development of the Lake Chad basin rest? The perspective of state sovereignty will suggest Nigeria, Cameroon, Chad, maybe Mali, and other interested West African nations to bear the brunt alone—regardless of their capacity to do so. A world polity theory perspective however will suggest differently [39, 40]. This framework highlights the global system as one social system with a cultural framework referred to as “world polity.” This takes into account every actor in the world system and influences international organizations like the UN, states as well as individuals affected by both. This social system is governed by principles and models that shape the course and objectives of social actors and what they do [39, 40]. From this perspective, the security situation in the LCB, negatively impacting the economy and adversely affecting the lives of millions, does have grave global import.
\nChanging the socioeconomic situation in the region and turning the fortunes of its inhabitants should give attention to the global and local nature of the causes and proposed solutions. Independently, and in collaboration, the affected states and global actors, like development organizations, nonprofits and other states within the region, can gradually effect change. Insecurity from insurgency affecting the region is a global phenomenon so is climate change causing drought in the LCB. Interventions designed around intelligence, capacity building, foreign direct investment, and environmental protection driven locally and influenced globally will be key to restoration in the Lake Chad Basin.
\nBiomedical application of nanotechnology has revolutionized tissue engineering as it can generate efficient biocompatible scaffolds with tuneable physico-chemical properties. Controllable biodegradability is one of the most important aspects as it supports the cells to produce extracellular matrix and promote effective healing. Likewise, adjustable pore structures of the scaffolds provides attractive site for loading drugs for resisting post-surgical infections and promoting cell attachment and colonization. Excellent biomechanical properties obtained by rational selection of the bioink help to mimic the tissue microenvironment and provide load bearing capacity to the tissue after repair [1]. Adherence of cells, proliferation and induction of osteogenic differentiation is higher when the total porosity of the 3D printed surfaces are more than 90% [2, 3]. Hence, such scaffolds with architectural specificity to the desired tissue like bone, cartilage, heart or nerves is immensely critical during implantation in order to ensure regeneration of the new tissue followed by repair [4].
Complete healing in traumatic injury is often a challenge that requires complicated surgical procedures which are often associated with failures and post-surgical infections. Till date bone grafting using autografts, allografts, xenografts, and synthetic bone grafts are employed for fixing the injury [5, 6]. However, the factors critical for success of grafting are the optimal size, shape, biomaterial and the anatomical structure of the bone defects. Thus, 3D printed scaffolds or synthetic bone grafts are considered more feasible due to their tuneable mechanical properties identical to the original bone tissue, and ease of rapid re-vascularization [7].
Weakening and gradual damage to cartilage may also lead to joint injury. Likewise, sudden traumatic injury, formation of lesions and developmental defects may also result is degradation of cartilage and impairment of its function [8]. In the United States alone, it is estimated that around 200,000–300,000 patients have undergone cartilage surgery [9]. It is important to note that the articular cartilage is non-neural, lymphatic, and avascular, having very low self-regenerating capacity [10]. Hence 3D printing mediated fabrication of scaffolds for repair or replacement is thought to be one of the most preferable technologies for cartilage tissue engineering [11]. Similarly, in treating cardiac dysfunctions, it is essential to maintain and mimic the cardiovascular anatomy while fixing the heart defects using tissue engineered vascular grafts (TEVGs). The 3D printing has tremendously helped to fabricate patient- and operation-specific vascular grafts [12]. Further, growing cases of neurodegenerative diseases also require effective therapeutic interventions, which are ideal for axonal regeneration and functional recovery for brain and spinal cord injury (SCI). Neuroregenerative scaffolds developed by 3D printing are considered as innovative materials that mainly focus on providing supportive substrates to guide axons and break the physical and chemical barriers, thereby promoting healing [13].
Collagen type 1 is most favorable for microextrusion based 3D bioprinting of biodegradable and biosorbable scaffolds. Collagen type 1 is the most predominant protein in the extracellular and intercellular matrix, constituting 20–30% of the vertebrate connective tissue, alongside hyaluronic acid (HA). Most importantly, the biocompatibility and low antigenicity of the collagen is attributed to the repeating motifs formed by the alpha chain of hydroxyproline-proline-glycine [14]. Collagen provides highly porous structure and hence permeability which in turn facilitates adhesion, migration, differentiation in addition to the regulation of the cellular morphology [15, 16].
This chapter highlights the collagen based 3D printed scaffolds with their attractive properties such as hydrophilicity, biodegradability, permeability, plasticity and biocompatibility critical for tissue engineering.
Biomaterials composed of collagen as listed in Table 1 are considered ideal substrate for 3D printing mediated fabrication of scaffolds for tissue engineering purposes [32]. However, simulation of the tissue microenvironment is crucial to mimic the physical and morphological properties of the native tissues in order to ensure proper restoration and replacement. The following section elaborates various advances of 3D bioprinting with collagen for tissue engineering.
Tissue | Biomaterials | Reference | |
---|---|---|---|
Bone | polycaprolactone (PCL), poly (lactic-co-glycolic acid) (PLGA), and β-tricalcium phosphate (β-TCP), atelocollagen | circular calvarial defects in male Sprague–Dawley rats | [17] |
Bone | calcium phosphate, Phosphoric acid, collagen | critically sized murine femoral defect | [18] |
Bone | Bioglass 45S5 (BG), methacrylated collagen (CMA) | human mesenchymal stem cells | [19] |
Bone | mesoporous bioactive glass (BG) microspheres with 4% molar percentage of strontium, Type I collagen | simulated body fluid (SBF) | [20] |
Bone | rod-like nano-hydroxyapatite particles embedded in a type I collagen matrix | — | [21] |
Cartilage | collagen, oligomeric proanthocyanidin, oxidized hyaluronic acid | rat bone marrow mesenchymal stem cells (rBMSCs), bone defects in skulls of the Sprague Dawley (SD) rat | [22] |
Cartilage | methacrylated gelatin (GelMA), nanohydroxyapatite (nHA) | bone marrow mesenchymal stem cells (BMSCs), rabbit osteochondral defect | [23] |
Cartilage | crude collagen extracted from tendons of skeletally mature rat tails | primary meniscal fibrochondrocytes | [24] |
Heart valve | gelatin support gel 3D printed with Lifeink® 200 | subcutaneous implantation in Sprague–Dawley rats | [25] |
Neonatal scale human heart | gelated collagen | human stem cell– derived cardiomyocytes | [12] |
Cardiac tissue | gelatin, gum arabic microparticles, rat collagen-I | human induced pluripotent stem cells (hiPSC)-cardiomyocytes | [26] |
Nerve | collagen, silk fibroin | neural stem cells (NSCs), spinal cord injury (SCI) in Sprague–Dawley rats | [27] |
Peripheral nerve | poly-lactic acid (PLA), collagen | PC-12 cells, Schwann cells, and primary chick dorsal root ganglia | [28] |
Elastic nerve guidance conduits (NGCs) | poly(lactide- | sciatic nerve injury models in rats | [29] |
Neural tissue | VEGF-releasing fibrin gel, Type I collagen | C17.2 cells | [30] |
Axon | chitosan, collagen | spinal cord injury (SCI) in rats | [31] |
Spinal cord | heparin sulfate, collagen | neural stem cells (NSCs) from embryonic day 14 (E14) brains, spinal cord injury (SCI) in rats | [13] |
Collagen based biomaterials for 3D printed tissues.
Collagen based scaffolds are widely used for bone tissue engineering. Hwang et al. (2017) fabricated bone grafts employing 3D printing using a composite of polycaprolactone (PCL), poly (lactic-co-glycolic acid) (PLGA), and β-tricalcium phosphate (β-TCP) mixed in a ratio of 4:4:2 [17]. Figure 1 shows the scanning electron microscope (SEM) images of the bone grafts. The bone graft developed by solid freeform fabrication (SFF) technique were further mixed with 3% atelocollagen and poured into a mold and incubated at 37°C for 15 min followed by deep freezing for 6 h and freeze drying for 12 h. The collagen based biomaterial was then immersed in ethanol/water (90% v/v) co-solvent containing 50 mM of 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide (EDC) and 20 mM of N-hydroxysuccinimide (NHS) for 24 h at room temperature for effective cross-linking. Each cross-linked collagen block had a diameter and height of 8 mm and 2 mm, respectively. Circular calvarial defects of 8 mm diameter were created by removal of periosteum in male Sprague–Dawley rats. The PCL/PLGA/β-TCP composite block bone grafts were implanted into the defect cites. Interestingly the bone grafts were surrounded by fibrous connective tissues. Subtle bone formation was noted while infiltration of the giant cell and inflammatory cells were seen. However, after eight weeks both neovascularization and new bone formation were noted around the bone grafts. It was speculated that these novel PCL/PLGA/β-TCP composite block bone grafts may be considered as an alternative to synthetic bone grafts.
SEM images of PCL/PLGA/β-TCP particulate bone grafts. (a) Well-defined PCL/PLGA/β-TCP particulate bone grafts were confirmed at a magnification of ×100; (b) rough surface of PCL/PLGA/β-TCP particulate hone grafts were observed at a magnification of ×800. Reprinted from Hwang et al. [
In another study, Inzana et al. tailored a composite scaffold using calcium phosphate and collagen for bone tissue regeneration [18]. Phosphoric acid at a concentration of 8.75 wt% was used as a binder that significantly improved the cellular viability. Tween 80 supplementation further enhanced the strength of the 3D printed scaffolds. Further, supplementation of the binder solution with 1–2 wt% collagen significantly enhanced the maximum flexural strength and cell viability. The pore size was in range from 20 to 50 μm that may significantly facilitate in-growth of the bone and reestablishment of the marrow compartment. The surface was covered by plate like crystal growth which increased the surface area significantly that is ideal for adsorption of drugs and/or proteins. On implanting the 3D printed scaffolds into a critically sized murine femoral defect for 9 weeks, promising osteoconductive properties were noticed.
Kajave et al. (2021) developed a bioactive ink composed of Bioglass 45S5 (BG) and methacrylated collagen (CMA) for 3D printing of biomimetic constructs for bone tissue engineering [19]. The bioink resembled native bone tissue in the organic and inorganic composition. Superior stability with minimum swelling of the collagen based hydrogel was achieved due to homogeneous dispersion of BG particles within the collagen network. Excellent rheological property was confirmed by the betterment in the yield stress. Similarly, incorporation of the BG resulted in improvement in the percent recovery of 3D printed constructs. Additionally, improved bone bioactivity of 3D printed constructs in stimulated body fluid was advantageous. Osteogenic induction and differentiation by BG incorporated CMA (BG-CMA) constructs was associated with high cell viability and enhanced alkaline phosphatase activity and calcium deposition in human mesenchymal stem cells.
In another interesting study, Montalbano et al. fabricated a hybrid bioactive material suitable for 3D printing of scaffolds mimicking the natural composition and structure of healthy bone [20]. Initially mesoporous bioactive glass (BG) microspheres with 4% molar percentage of strontium were synthesized. Thereafter, Type I collagen and strontium-containing mesoporous BG were combined to obtain suspensions able to perform a sol–gel transition under physiological conditions. The fibrous nanostructures were homogeneously distributed embedding inorganic particles as evident from the field emission scanning electron microscopy (FESEM). Large calcium phosphate deposition was observed while release of strontium ions from the embedded BG was attributed to the high-water content of the composite. These features can cumulatively promote the osteogenic induction which is significant for bone tissue engineering. On soaking the composite scaffolds in simulated body fluid (SBF), hydroxyapatite (HA) crystals were uniformly distributed along the cross section of the sample that increased with time from 3rd to 7th day as evident from Figure 2.
Cross-sectional FESEM images showing HA crystal deposition on collagen/MBG_Sr4% samples after three and seven days of incubation in SBF at different magnifications. Reprinted from Montalbano et al. [
In subsequent study Montalbano et al. reported composite biomimetics comprised of rod-like nano-hydroxyapatite particles embedded in a type I collagen matrix [21]. This composite was developed to mimic the bone composition. Initially a hydrothermal method using 0.2% ammonium-based dispersing agent (Darvan 821-A) was employed for the fabrication of the HA nanorods that were uniform-sized with length of 40–60 nm and a width of 20 nm. On suspending this material in a collagen solution in presence of Darvan 821-A, a uniform collagen/nano-HA suspension was obtained that was ideal for extrusion 3D printing. The mesh-like structures printed in a gelatine-supporting bath led to fabrication of 3D bone-like scaffolds.
One of the most prevalent tissue damages suffered by adults, children and adolescents is articular cartilage defects. In severe cases degenerative joint diseases may result due to exposure of bone terminals caused by progressive wear and tear of articular cartilage. However, low rate of tissue regeneration and self-repairing capacity poses a challenge for effective healing and restoration of the function. Several collagen based 3D scaffolds are being developed for inducing cartilage regeneration that is discussed in detail in this section. Recently, Lee et al. fabricated a highly biocompatible collagen/oligomeric proanthocyanidin/oxidized hyaluronic acid (C/OPC/OHA) composite scaffold with superior compressive strengths between 0.25–0.55 MPa [22]. The composite scaffolds were 3D printed using four types of needles, 25G red plastic, 22G blue plastic, 25G red metal, and 22G blue metal to achieve 20%, 25%, and 30% porosities when pressure of 25, 15, 125, and 100 kPa were applied, respectively as illustrated in Figure 3. Porous nature of the scaffolds is advantageous for promoting both angiogenesis and cartilage ossification. The minimum and maximum storage moduli of the hydrogel were approximately 2.6 kPa and 4.1 kPa, respectively. Interestingly, an increased degradation rate of the composites was 26.6%, 30%, and 30.7% for 0, 5, and 10 mg/mL of OHA, respectively after 49 days. Higher apatite deposition on the scaffold surface was evident on day 21 on immersion in simulated body fluid. Superior cell viability (up to 90%) was achieved when rat bone marrow mesenchymal stem cells (rBMSCs) were grown on the composite scaffolds. On implantation of the scaffolds into bone defects in skulls of the Sprague Dawley (SD) rat, angiogenesis and new bone formation was evident that indicated 3D collagen-based scaffolds could be used as potential candidates for articular cartilage repair.
Optimization of 3D bioprinting parameters for obtaining porosity at 20%, 25%, and 30% using different needle densities (25G red plastic and metal, 22G blue plastic and metal) and different pressures (25, 15, 135, and 100 kPa). Reprinted from Lee et al. [
Liu et al. developed a tri-layered scaffold employing extrusion-based multi-nozzle 3D printing technology where the bioink was comprised of 15% methacrylated gelatin (GelMA) hydrogel for cartilage on top layer, a combination of 20% GelMA and 3% nanohydroxyapatite (nHA) (20/3% GelMA/nHA) hydrogel for interfacial layer, and a 30/3% GelMA/nHA hydrogel for subchondral bone at bottom layer [23]. The composite was biodegradable with maximum degradation (61.4%) in 14 days. Interconnected microtubule-like structure of each layer with interconnected spherical pores with a size of about 300 μm was observed. The Young’s modulus increased with the increase in GelMA concentration in the scaffold. The scaffolds were biocompatible with the bone marrow mesenchymal stem cells (BMSCs) while they exhibited effecting healing of rabbit osteochondral defect. Higher cartilage-specific extracellular matrix formation and collagen type II were observed on treatment with the tri-layered scaffolds. Further, effective new tissue formation and even integration with the surrounding tissues indicated their promises for repair of damages in subchondral bone by inducing cartilage regeneration.
In an interesting study, Rhee et al. fabricated 3D printing assisted soft tissue implants with high-density collagen hydrogels as illustrated in Figure 4 [24]. External heating and collagen concentrations of 12.5, 15, and 17.5 mg/mL enhanced the shape fidelity. At the highest printable concentration, the modulus of printed gel was ̴ 30 kPa. Cell viability within the tissue constructs was high and no notable decrease was observed even after 10 days of culturing. Higher infiltration of the fibochondrocytes cells throughout the collagen matrix was found by 10 days. Adherence of the cells on the outer surface of the nascent collagen fibers was prominent while very few cells colonized the spaces between the fibers.
Printing process of sheep meniscus, (a) CT scan of meniscus, (b) print path of meniscus deposition of collagen hydrogel during printing, (c) 3D printed meniscus. (d) Geometry assessment of constructs. (e) Constructs scanned using Cyberware 3D scanner. (f) Geometry of the test construct: Half-cylinder. Reprinted with permission from Rhee et al. [
Cardio-vascular defects such as aortic valve disease (AVD) require high precision surgical procedure that include either mechanical or bioprosthetic valve replacement. Recently, tissue engineered heart valves (TEHV) have gained more attention that are effectively achieved by 3D bioprinting. Maxson et al. evaluated the recellularization potential of 3D-bioprinted scaffold and investigated its applicability as a heart valve implant [25]. Allogenic rat mesenchymal stem cells (rMSCs) with green fluorescent protein (GFP) label were grown and mixed with Lifeink® 200 to obtain a homogenous bioink. Thereafter, a computer aided design (CAD) model for the implant disk scaffolds was prepared wherein the dimensions of the scaffold facilitated easy implantation and mounting in order to avoid migration and folding. Neovascularization was observed after 4 weeks with integration of host tissues with the bioink explants. Moreover after 8 weeks, minimal difference between the two layers was observed; however, the structural integrity of the extracellular matrix (ECM) was maintained. Furthermore, Mason’s trichome revealed fibrosis on the cutaneous side of the explant whereas CD3 and CD163 biomarkers demonstrated chronic inflammation as well as ECM remodeling whose expressions were decreased with subsequent increase in incubation period. CD163 displayed a steady reduction in expression from week 1 to week 8, respectively. On the other hand, CD31 biomarker expression was considerably increased within the same time period due to endothelialization and angiogenesis. The vimentin (a major intermediate filament of smooth muscle cells) concentration of surrounding tissues was also increased with improvement in elastin concentration. This was attributed to the infiltration of the Bioink by interstitial-like cells. In addition, the ultimate tensile strength (UTS) was decreased from 0.344 ± 0.120 MPa in the second week to 0.169 ± 0.077 MPa in the fourth week while it was increased to 0.275 ± 0.166 MPa in the eighth week. Likewise, the tensile modulus was also reduced from 1.186 ± 0.872 MPa in the second week to 0.548 ± 0.341 MPa in the fourth week followed by an increase to 1.425 ± 0.620 MPa in the eighth week. Elastin concentration was significantly increased in the fourth week. Post eight weeks of implantation, expression of CD31 biomarker continued to decrease while CD163 expression increased in week 12 which was attributed to M2 macrophage infiltration. Additionally, the bioink explant was encapsulated by the fibrotic tissue within week 12 while UTS was further increased within this time period. Enhanced levels of both vimentin and elastin indicated strengthening of the extracellular matrix in the bioprinted scaffold due to active collagen deposition. Hence, collagen-based bioink application was demonstrated to be efficient for formation of heart valves.
In another study, Lee et al. also demonstrated 3D bioprinting of collagen for human heart engineering [12]. Herein, 3D bioprinting was carried out using a second generation of the free form reversible embedding of suspended hydrogels (FRESH v2.0) that provides support for printing and then subsequently melts away at 37°C. Moreover, uniform gelatin microparticles with spherical morphology (with diameter ̴ 25 μm) reduced polydispersity. An optimal balance between the resolution of individual strand and strand-to-strand adhesion was further maintained using a 50 mM N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES) buffered bath with pH 7.4 which in turn facilitated multiple bioink printing. A linear small coronary artery-scale tube was then fabricated using collagen type I perfusion system with an inner diameter and wall thickness of 1.4 mm and 300 μm, respectively. Thereafter, C2C12 cells were perfused in the tube that displayed viability along with active remodeling of the gel after five days. Further, cellular infiltration was also analyzed using fabrication of collagen disks with a thickness of 5 mm and a diameter of 10 mm wherein excessive cellular infiltration as well as collagen remodeling was observed post three days of implantation in the printed collagen as compared to solid-cast collagen. Moreover, fibronectin and vascular endothelial growth factor (VEGF) were incorporated into the bioink for enhanced vascularization. An extensive vascular network was observed in the printed collagen disk with red blood cells and CD31-positive vessels having a diameter range of 8–50 μm. Thereafter, collagen bioink was used along with human stem cell-derived cardiomyocytes to FRESH print a left ventricle model wherein around 96% post-printing cell viability was achieved through rapid collagen neutralization. A dense layer of interconnected and striated human embryonic stem cell-cardiomyocytes (hESC-CMs) was obtained after seven days of culturing. A baseline spontaneous ventricle beat rate of around 0.5 Hz was captured that was paced at 1 and 2 Hz using field stimulation. Furthermore, the mechanical integrity of the constructs was demonstrated using a 28 mm tri-leaflet heart valve that was robust enough to withstand air pressure. In addition, a neonatal-scale human heart was also printed using collagen bioink that highlighted the potential of FRESH v2.0 printing technique for fabrication of advanced tissue scaffolds for other organ systems as well.
Collagen-based bio-ink was also demonstrated to be an effective tool for direct 3D printing of human induced pluripotent stem cells (hiPSC)-cardiomyocytes that could then be utilized for cardiac tissue engineering [26]. Cardiomyocytes were differentiated in a 2D monolayer followed by CHIR99021-treatment mediated cell expansion and regular passing. Later on, a rat collagen-I based bioink was used for the encapsulation of cells followed by printing in a support bath composed of complex coacervate gelatin/gum arabic microparticles. The bioink was then gelated at 37°C and cultivated under free-floating conditions for a time period of thirty days. Ring-shaped cardiac tissues were printed with 5 × 5 × 1 mm dimension wherein the initial contractions were seen post three days of culturing. Striated sarcomeres were demonstrated with significant responsiveness toward pharmacological stimulations. Therefore, this study demonstrated potential of cardiac tissue engineering with enhanced properties and functions through 3D-bioprinting.
Scaffolds rationally fabricated employing 3D bioprinting could help in the treatment of spinal cord injury (SCI) by nerve tissue engineering. In a study by Jiang et al., Collagen/silk fibroin scaffold was 3D bioprinted and combined with neural stem cells (NSCs) to promote nerve regeneration [27]. A collagen/silk fibroin ratio of 4:2 was used for scaffold preparation using a 3D-bioprinter with a nozzle diameter of 210 μm, printing speed of 9 mm, extrusion speed of 2-mm/min, 0.1 mm thickness and a platform temperature of −20°C. Characterization of the 3D bioprinted scaffolds in rats revealed complete degradation of the composite scaffold after 4 weeks of implantation. Furthermore, the scaffold had considerable ductility as well as compression resistance with a compressive elastic modulus of 60.05 ± 5.12 kPa. Fourier transform infrared (FTIR) spectroscopy results then revealed presence of absorption peaks at 3445.7, 2932.46, 1640.58, and 1376.45 cm−1 that corresponded with -OH or -NH peak, methyl or C-H stretching vibrations of methylene group, C=O or C=C stretching vibrations, and saturated C-H bending vibration, respectively. Hence, these functional groups suggested presence of suitable lipid- and water-soluble bonds in the 3D bioprinted scaffold that may facilitate adhesion and growth of nerve cells. Moreover, significant biocompatibility between the scaffold and NSCs were attained with evenly distributed micropores and pore connections in the scaffold as observed in scanning electron microscopy (SEM) images. Fusiform-shaped cells grew in the scaffold pores, while some cells grew densely on the scaffold surface with extended pseudopods facilitating cell adhesion, growth as well as provided a carrier and channel for regeneration of the nerve fibers. Hence, a conducive microenvironment for NSC adhesion, growth and differentiation was provided by the 3D-bioprinted scaffold. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay also demonstrated successful seeding and proliferation of the NSCs on the scaffold. Thereafter, behavioral changes at the spinal cord injury site were investigated after implantation of the scaffold. The Basso-Beattie-Bresnahan (BBB) open-field locomotor score of the group implanted with 3D-collagen/silk fibroin scaffolds and NSCs was higher as compared to the control after 8 weeks of surgery. In addition, motor function recovery was better in groups having the scaffold and NSCs. Similarly, electrophysiological studies revealed prominent recovery in groups having 3D bioprinted scaffold along with NSCs as compared to control groups. Left hind limb amplitude was significantly higher in scaffold group when compared with control after 1 month of surgery. In addition, magnetic resonance imaging (MRI) and diffusion tensor imaging revealed improved filling of the injury cavity, enhanced spinal cord continuity, increased regenerative axons as well as reduced glial scarring in groups implanted with the scaffold and NSCs.
In another study, Li and Gao fabricated 3D microtubular collagen scaffolds and investigated its potential in peripheral nerve repair [28]. Melt spinning or 3D printing using poly-lactic acid (PLA) was carried out to obtain fibrous template material with a diameter range of 50–100 μm that was then utilized for fabrication of collagen scaffolds. Microtubules were prepared by parallel stacking of melt spun PLA fibers followed by polymerization of the collagen whereas PLA fibers with a diameter of 200 μm and 100 μm interspacing was fused and deposited using 3D printing. The thickness of inner ranged from 10 to 20 μm while the exterior wall formed a shell with a thickness of about 70 μm. Furthermore, cell adhesion ability of adrenal phaeochromocytoma (PC-12) and D62PT Schwann cells was evaluated wherein the cells firmly attached to native as well as chloroform-exposed Matrigel films. Two crosslinkers namely, 0.3% genipin and 0.3% glutaraldehyde were used that decreased swelling as well as enzymatic degradation of the Matrigel. Untreated gels demonstrated retention of 34.5% of total mass after 24 h incubation with 0.05% collagenase, whereas genipin and glutaraldehyde treated gels showed total mass retention of 96.7% and 99.3%, respectively. PC-12 and D62PT Schwann cells further showed well adherence and confluent growth onto microtubule scaffolds after 10 and 4–5 days of culturing, respectively. Moreover, a strong alignment of cells as well as formation of channels was seen in Schwann cells while primary chick dorsal root ganglia displayed neurite growth along the major axis of the microtubes.
Likewise, Yoo et al. reported fabrication of elastic nerve guidance conduits (NGCs) using poly(lactide-
Lee et al. also demonstrated bio-printing of collagen and VEGF-releasing fibrin gel scaffolds and investigated its potential in artificial neural tissue construction [30]. Murine neural stem cells (NSCs) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and further used for cell printing. Type I collagen was then prepared and 1.16 mg/mL of the collagen scaffold was used for 3D bio-printing of C17.2 cell-scaffold complex. An average of 56 ± 9 cells/droplet was obtained with a cell viability of 93.23 ± 3.77% which was similar to that of manually-plated cells. Moreover, a collagen scaffold concentration of 1.74 mg/mL demonstrated highly dense and proliferating cells with a viability of 96.72 ± 3.58% after 3 days of culturing. Furthermore, the combinatorial effect of collagen scaffold and VEGF-containing fibrin gel on C17.2 cells was investigated wherein, the cell morphology altered after two days of culturing with active proliferation and formation of clusters. In addition, the cells located near the fibrin gel border gradually migrated toward the VEGF-containing fibrin gel and continued differentiation. After three days of culturing, the total migration distance was 102.4 ± 76.1 μm. Hence, proper cell proliferation and migration was displayed using the two scaffolds which highlighted the potential of 3D bioprinting in artificial tissue construction.
Likewise, axon regeneration was ameliorated by Sun et al. using 3D printed collagen/chitosan scaffolds [31]. A 3D bioprinter was used for fabrication of the scaffold that had an interconnected porous structure with a porosity of 83.5% as observed in SEM images. The pore size of the scaffold ranged from 60 to 200 μm. Hence, significant space was obtained by the cells for growth and adherence. The compressive modulus of 3D collagen/chitosan scaffold was 3.82 ± 0.25 MPa along with enhanced compressive strength of 345.20 ± 29.60 KPa. The cytocompatibility of 3D printed scaffolds was similar to that of scaffolds prepared using freeze drying technology. Interestingly, the persistent locomotion recovery as well as significant increase in blood brain barrier (BBB) scores was observed after implantation of the 3D printed collagen/chitosan scaffolds in rats with spinal cord injury (SCI). Moreover, the magnetic resonance and diffusion tensor imaging results revealed a significant signal increase at the epicenter of the spinal cord lesion in rats implanted with 3D printed collagen/chitosan scaffold. Post eight weeks of SCI surgery, the axonal regeneration was demonstrated wherein 3D collagen/chitosan implantations resulted in amplitude and latency improvement. Further confirmation of axonal regeneration was carried out using anterograde biotin dextran amine (BDA) labeling wherein BDA-positive fibers were observed in 3D collagen/chitosan implantations. Hematoxylin and eosin (HE) staining also demonstrated linear ordered structure of the spinal cord after eight weeks with no obvious cavity observed in 3D printed collagen/chitosan implanted group whereas visible cavities and disordered structures were observed in injury groups. Hence, 3D printed scaffolds were demonstrated to be effective in axon regeneration and amelioration of spinal cord injury.
In a similar study, Chen et al. constructed collagen/heparin sulfate based scaffolds using 3D bioprinting and evaluated its action in functional SCI recovery in rats [13]. The scaffold was prepared using a 3D bioprinter that had a cylindrical morphology with a uniform and regular internal structure along with high porosity as observed in SEM images. The compressive modulus of 3D printed collagen/heparin sulfate was 3.46 ± 0.278 MPa which was higher as compared to scaffolds prepared using freeze drying technology. Likewise, enhanced compressive strength of 308.9 ± 28.65 KPa was observed in 3D printed scaffold. Furthermore, release profile of basic fibroblast growth factor (bFGF) from 3D printed scaffold was also evaluated wherein scaffolds prepared using freeze drying method demonstrated an initial burst of 54.89% of bFGF was released in the first day after which a slow release behavior was observed for longer time period. However, a steady bFGF release behavior was observed in case of 3D printed scaffolds for twenty days. Thereafter, the biocompatibility of scaffolds was analyzed using NSCs which proliferated inside the pore followed by spreading on the wall of the scaffolds. In addition, MTT assay revealed no significant difference in cell growth on different scaffolds thus highlighting the cytocompatibility of the 3D printed collagen/heparin sulfate scaffolds. Implantation of the 3D printed scaffolds further demonstrated significant recovery of locomotor functions in rats after two months with amelioration of the SCI as well as enhanced number of neurofilament positive cells.
Advances in the field of nanomedicine have enabled exploration of novel biomaterials for tissue engineering. Among various biopolymers such as, chitosan, alginate, silk fibrion, collagen is considered as most attractive due to its biocompatibility and biodegradability. However, high temperature and extreme conditions during fabrication and bioprinting results in low stability of the collagen molecules. Hence, ideal porous scaffolds should involve combination of type I collagen and hydroxyapatite particles by freeze-drying. It is essential to have tuneable pore dimensions for superior ingrowth of cells and blood vessels. More complex microarchitectures of the collagen based scaffolds with specific rheological properties such as shear thinning, yield stress and fast shear recovery can be obtained using extrusion-based 3D printing [33].
Various biologically synthesized nanoparticles like silver, gold, copper, platinum, palladium and others can be supplemented in the scaffolds resisting post-surgical microbial infections [34, 35, 36, 37]. Biofilm associated infections are most challenging to treat and are highly responsible for implant failure. Hence, coating of implants with antimicrobial nanoparticles impregnated collagen can be an effective strategy to increase the shelf life of the implants [38, 39]. Also drug functionalized nanoparticles can be embedded in the collagen matrix to ensure sustained release and rapid healing of the injured tissues.
Multiple approaches and integration of medical biology and material science will certainly help to revolutionize regenerative medicine by rational tissue engineering. In view of the background collagen based 3D printed scaffolds hold tremendous potential as candidate nanotherapeutics.
Dr. Sougata Ghosh acknowledges Kasetsart University, Bangkok, Thailand for Post Doctoral Fellowship and funding under Reinventing University Program (Ref. No. 6501.0207/10870 dated 9th November, 2021).
The authors declare no conflict of interest.
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Delac received his B.Sc.E.E. degree in 2003 and is currentlypursuing a Ph.D. degree at the University of Zagreb, Faculty of Electrical Engineering andComputing. His current research interests are digital image analysis, pattern recognition andbiometrics.",institutionString:null,institution:{name:"University of Zagreb",country:{name:"Croatia"}}},{id:"557",title:"Dr.",name:"Andon",middleName:"Venelinov",surname:"Topalov",slug:"andon-topalov",fullName:"Andon Topalov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/557/images/1927_n.jpg",biography:"Dr. Andon V. Topalov received the MSc degree in Control Engineering from the Faculty of Information Systems, Technologies, and Automation at Moscow State University of Civil Engineering (MGGU) in 1979. He then received his PhD degree in Control Engineering from the Department of Automation and Remote Control at Moscow State Mining University (MGSU), Moscow, in 1984. From 1985 to 1986, he was a Research Fellow in the Research Institute for Electronic Equipment, ZZU AD, Plovdiv, Bulgaria. In 1986, he joined the Department of Control Systems, Technical University of Sofia at the Plovdiv campus, where he is presently a Full Professor. He has held long-term visiting Professor/Scholar positions at various institutions in South Korea, Turkey, Mexico, Greece, Belgium, UK, and Germany. And he has coauthored one book and authored or coauthored more than 80 research papers in conference proceedings and journals. 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The situation in, for example, Sweden and South Africa faces huge issues concerning an increased lack of teachers in the future, as well as difficulties with teacher attrition. Examples from the USA show that up to 50% of new teachers quit working as teachers within 5 years. The difficulties with knowledge transfer to new generations, are a global threat. In this chapter, the deficiency of examined teachers is addressed in Section 1. This is followed by an overview of two key aspects of teachers’ professional development, namely bridging the gap between practice and theory to enhance teaching quality as well as the importance of practice-based professional development to maintain teachers to work as teacher in a long-term perspective. The results of a case study of students’ views on theories are presented, showing a strong experienced dichotomy between theory and practice, and difficulties to see how theories could be used to better understand classroom situations. Finally, a proposal of how to bridge the gap between theory and practice with designed modules is presented.",book:{id:"7804",slug:"teacher-education-in-the-21st-century",title:"Teacher Education in the 21st Century",fullTitle:"Teacher Education in the 21st Century"},signatures:"Mona Holmqvist",authors:[{id:"275455",title:"Dr.",name:"Mona",middleName:null,surname:"Holmqvist",slug:"mona-holmqvist",fullName:"Mona Holmqvist"}]},{id:"70433",doi:"10.5772/intechopen.90502",title:"The Moral Role of Pedagogy as the Science and Art of Teaching",slug:"the-moral-role-of-pedagogy-as-the-science-and-art-of-teaching",totalDownloads:1129,totalCrossrefCites:6,totalDimensionsCites:6,abstract:"The purpose of this chapter is to present the key concepts and actors in pedagogy and didactics in the context of institutional teaching. We present a holistic approach to education and view human beings as lifelong learners who need to be educated comprehensively to actualize their full potential. In this chapter we discuss how pedagogy, the science and art of teaching, can promote the educational goals identified in the curriculum. In this chapter we adhere to the Didaktik curriculum tradition in which values and morals are emphasized in guiding the teaching-studying-learning process. This means that pedagogy is moral in nature, and the teacher’s main task is to reflect the values underlying her teaching and the purposes she wants to advance in her teaching. We also discuss the current pedagogical challenges in both basic and higher education in educating students for the twenty-first century.",book:{id:"9040",slug:"pedagogy-in-basic-and-higher-education-current-developments-and-challenges",title:"Pedagogy in Basic and Higher Education",fullTitle:"Pedagogy in Basic and Higher Education - Current Developments and Challenges"},signatures:"Kirsi Tirri and Auli Toom",authors:[{id:"234399",title:"Prof.",name:"Kirsi",middleName:null,surname:"Tirri",slug:"kirsi-tirri",fullName:"Kirsi Tirri"},{id:"298433",title:"Dr.",name:"Auli",middleName:null,surname:"Toom",slug:"auli-toom",fullName:"Auli Toom"}]}],mostDownloadedChaptersLast30Days:[{id:"69139",title:"Formative Assessment in Mathematics Education in the Twenty-First Century",slug:"formative-assessment-in-mathematics-education-in-the-twenty-first-century",totalDownloads:1277,totalCrossrefCites:2,totalDimensionsCites:2,abstract:"Assessment does not always have to involve paper and pencil but can instead be a project, an observation, or a task that shows a student has acquired the concept and can make sound connections and linkages with other related concepts. Learning is meaningful when the student comprehends the relationship of what is being learned to other knowledge. Furthermore, concept map measures aspects of learning, which conventional tests cannot measure such as students’ misconceptions. As such, the chapter shall focus on formative assessment in mathematics classroom mediated by a method of teaching (concept mapping) that promotes critical thinking, which assists teachers to teach and assess students’ understanding and make connections between concepts explicitly.",book:{id:"8039",slug:"theorizing-stem-education-in-the-21st-century",title:"Theorizing STEM Education in the 21st Century",fullTitle:"Theorizing STEM Education in the 21st Century"},signatures:"Benard Chigonga",authors:[{id:"298587",title:"Dr.",name:"Benard",middleName:null,surname:"Chigonga",slug:"benard-chigonga",fullName:"Benard Chigonga"}]},{id:"68547",title:"The Role of Mathematical Modeling in STEM Integration and Education",slug:"the-role-of-mathematical-modeling-in-stem-integration-and-education",totalDownloads:1246,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"With the rapidly developing technology, the labor force of the society has changed direction, and in the age of informatics, creative engineering applications have come to the forefront. Accordingly, the education levels of the labor force were also changed. The science, technology, engineering, and mathematics (STEM) education model in most countries aims to teach science, mathematics, technology, and engineering in relation to primary, secondary, high school, and higher education. STEM education, which has an impact in our country in recent years, has an important role in acquiring new skills, supporting creativity, innovation, and entrepreneurship, gaining the ability to transition between professions and adapting to new occupations. Nowadays, technology is expected to have different skills from individuals who will work in different fields with rapid development. Also, different teaching strategies play a major role in STEM integration and training. One of them, mathematical modeling, is the process of analyzing real-life or realistic situation using mathematical methods in the most general sense. The idea that mathematical modeling cycles should be used in STEM education at all levels from primary to tertiary education has gained importance in recent years, since it increases the students’ motivation towards the lesson and they learn better by concentrating their attention.",book:{id:"8039",slug:"theorizing-stem-education-in-the-21st-century",title:"Theorizing STEM Education in the 21st Century",fullTitle:"Theorizing STEM Education in the 21st Century"},signatures:"Murat Tezer",authors:[{id:"299964",title:"Associate Prof.",name:"Murat",middleName:null,surname:"Tezer",slug:"murat-tezer",fullName:"Murat Tezer"}]},{id:"66203",title:"Including Students with Disabilities in a Physical Education Teacher Preparation Program: An Institutional Perspective",slug:"including-students-with-disabilities-in-a-physical-education-teacher-preparation-program-an-institut",totalDownloads:1943,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"The increasing number of students with disabilities who have the goal of becoming a teacher in either elementary or high school is one of the challenges we are currently facing at the academic colleges of education in Israel. In this chapter, we address the inclusion challenge, namely how we have taken up the challenge to modify one existing teacher preparation program (TPP) in physical education (PE) to enable students with disabilities to study at the same level as the other students who are enrolled in the program. The chapter is composed of four sections. In the first section, we introduce the term inclusive education, elaborate upon its concepts, and highlight a number of developmental phases associated with this term. In the second section, we present the theoretical background and the practical frameworks of an inclusive pedagogy. In the third section, we describe a number of actions taken in one college that enabled students with disabilities to enroll in a PE TPP. In the fourth section, we conclude our discussion and provide a number of ideas for future research, in order to strengthen the understanding of how to integrate students with disabilities in PE TPP.",book:{id:"7804",slug:"teacher-education-in-the-21st-century",title:"Teacher Education in the 21st Century",fullTitle:"Teacher Education in the 21st Century"},signatures:"Ronnie Lidor and Yeshayahu Hutzler",authors:[{id:"293567",title:"Dr.",name:"Ronnie",middleName:null,surname:"Lidor",slug:"ronnie-lidor",fullName:"Ronnie Lidor"},{id:"293600",title:"Prof.",name:"Yeshayahu",middleName:null,surname:"Hutzler",slug:"yeshayahu-hutzler",fullName:"Yeshayahu Hutzler"}]},{id:"69651",title:"Parental Engagement in Children’s Learning: A Holistic Approach to Teacher-Parents’ Partnerships",slug:"parental-engagement-in-children-s-learning-a-holistic-approach-to-teacher-parents-partnerships",totalDownloads:1389,totalCrossrefCites:5,totalDimensionsCites:7,abstract:"This study presents the standpoint of parental engagement, conceptualized by Janet Goodall and collaborators, as a framework that is coherent to the principles of the holistic approach of pedagogy to teacher-parents’ partnerships. We bring forward the evolution of the concept of parental engagement and its main standpoints, in relation to more traditional theories on parental involvement. We also discuss previous findings about teachers’ and parents’ roles in education and teacher-parents’ partnerships, as well as how do changes in educational paradigms challenge home-school collaboration. Finally, the article highlights the need to implement research-based parental engagement practices in educational systems around the world.",book:{id:"9040",slug:"pedagogy-in-basic-and-higher-education-current-developments-and-challenges",title:"Pedagogy in Basic and Higher Education",fullTitle:"Pedagogy in Basic and Higher Education - Current Developments and Challenges"},signatures:"Cristiana Levinthal de Oliveira Lima and Elina Kuusisto",authors:null},{id:"55240",title:"Challenging Performativity in Higher Education: Promoting a Healthier Learning Culture",slug:"challenging-performativity-in-higher-education-promoting-a-healthier-learning-culture",totalDownloads:1789,totalCrossrefCites:1,totalDimensionsCites:2,abstract:"The nature and context of education have changed dramatically in recent decades. The increased prioritisation of standardisation, performance indicators and metrics often means that holistic, affective and wellbeing education are seen as less important in the educational endeavour. The value of education for education's sake is under siege. Previous emphasis on the education of the whole person (i.e., moral and creative aesthetic development) is often replaced by a more functionalist perspective of education as servicing economic need and global capitalist interests. Marketization of education has increased at an exponential rate and has had an adverse impact on the health and well‐being of both educators and students. This chapter elucidates how the triad of assessment, student well‐being and academic well‐being intersects in the ever increasing performative and neo‐liberalist cultures of higher education. It demonstrates the reciprocal dynamic of stress that is becoming more and more evident among educators and students. The chapter makes the case for more empowering and human‐centred educative contexts in order to facilitate better educational outcomes for students and healthier outcomes for all involved in the educational endeavour.",book:{id:"5773",slug:"global-voices-in-higher-education",title:"Global Voices in Higher Education",fullTitle:"Global Voices in Higher Education"},signatures:"Christine Deasy and Patricia Mannix‐McNamara",authors:[{id:"198284",title:"Dr.",name:"Christine",middleName:null,surname:"Deasy",slug:"christine-deasy",fullName:"Christine Deasy"},{id:"198290",title:"Dr.",name:"Patricia",middleName:null,surname:"Mannix McNamara",slug:"patricia-mannix-mcnamara",fullName:"Patricia Mannix McNamara"}]}],onlineFirstChaptersFilter:{topicId:"1316",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"81937",title:"Socialization Experiences among Undergraduate Students in Higher Learning Institutions (HLI)",slug:"socialization-experiences-among-undergraduate-students-in-higher-learning-institutions-hli-",totalDownloads:19,totalDimensionsCites:0,doi:"10.5772/intechopen.99007",abstract:"This work portrays the problems of socialization among undergraduate students in higher learning institutions. The socialization processes in higher learning institution are significant for the successful navigation of students in the academic programs and university environment in preparing the next generation of professional practitioners and scholars. But the undergraduate student socialization experiences of students at university environment are overlooked. To navigate in the higher learning institutions, students should be socialized effectively to the normative contexts of the higher learning institutions. The normative contexts of the higher learning institutions are generally categorized into social and academic contexts, because these context academic and social context integration have been linked to student retention and success. Social integration involves interpersonal relationships, support, interactions with others, and a sense of belonging at a university, which stems from extracurricular activities, informal dealings with peer groups, and interactions with faculty and staff, whereas academic integration is described through grade performance and intellectual development that reflects an ability to meet the standards of the academic system; intellectual development involves a student valuing their education as a process of development in which they gain knowledge and ideas. Students’ background is also the contributing factor for students’ socialization in the University.",book:{id:"10911",title:"Higher Education - New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg"},signatures:"Mulusew Birhanu Ayalew"},{id:"80280",title:"Adoption of Online Learning during the Covid19 Pandemic Lockdown by Universities in Garowe",slug:"adoption-of-online-learning-during-the-covid19-pandemic-lockdown-by-universities-in-garowe",totalDownloads:88,totalDimensionsCites:0,doi:"10.5772/intechopen.99941",abstract:"In response to the Covid-19 outbreak the world closed and therefore countries like Somalia have not been exceptional. The government of Somalia and all higher education institutions adopted crisis intervention measures on implementation of blended learning approaches like online teaching and learning. In this chapter we explore the process and challenges of adopting online learning in response to the world wide lockdown due to the pandemic. Given that this was an abrupt requirement, the survey was interested in finding out whether universities adopted and adapted easily. Researchers compared findings from previous studies and theoretical inclinations on online learning. Results indicate that the adoption of online learning among universities in Garowe was as a matter of crisis management whereby administration, lecturers and students were all not ready and had no prior grounding in this pedagogical learning platform. Just like previous studies online learning implementers have continued to encounter several challenges like intermittent internet network, cost of gadgets and facilities, inadequate skills of both the instructors and students, aspects of communication and satisfaction from stakeholders. With the research survey in Garowe, results show that this is still pervading and therefore need for more rigorous contextualised research on this subject.",book:{id:"10911",title:"Higher Education - New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg"},signatures:"Tumwebaze Alicon Auf and Omer Abdi Hamdi"},{id:"78597",title:"Public-Private Participation in Funding University Education in Sub-Saharan Africa: A Nigerian Case-Study for Sustainable Development",slug:"public-private-participation-in-funding-university-education-in-sub-saharan-africa-a-nigerian-case-s",totalDownloads:134,totalDimensionsCites:0,doi:"10.5772/intechopen.99940",abstract:"The developing countries in Africa still cannot withstand the pressure of the highly competitive global education market. Together with the large numbers of people who make a living in various innovative companies, these countries have solved key contemporary issues affecting global education. For this reason, it is necessary to actively respond to current technological innovation and educational challenges and to eliminate new technology graduates who can effectively interact with students through the responsive expansion of education and training. Expansion of education can produce effective expansion that promotes educational development, but due to budget constraints, most African governments cannot successfully and sustainably implement such educational programs. This is difficult. However, public-private partnership efforts provide a way out of this financial dilemma. The Sub-Saharan Africa initiative has achieved important educational objectives, such as: ensuring relevance for quality; secure funding for sustainability and establish resource mobilization partnerships and connections; and promote international cooperation. This discussion is relevant to the basic conditions for a successful public-private partnership with educational institutions and extended education and sheds light on the impact, lessons, and challenges. The public is increasingly concerned about the importance of higher education in the 21st century. This chapter explores some of the key functions of an innovative education system that supports the development of education in Nigeria and enhances people’s ability to use information. Nigeria’s education system re-emphasizes the importance of public and private universities, but the country does not have a sustainable education system and well-equipped educational institutions to support people’s ability to use information, learning, education, and research activities.",book:{id:"10911",title:"Higher Education - New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg"},signatures:"Lawrence Jones-Esan"},{id:"79197",title:"University Teachers’ Conceptions of What University Is: Implications for the Future of Higher Education",slug:"university-teachers-conceptions-of-what-university-is-implications-for-the-future-of-higher-educatio",totalDownloads:107,totalDimensionsCites:0,doi:"10.5772/intechopen.100813",abstract:"This chapter presents the perception of university teachers about the university, the most recent changes and how they have influenced their activity. The phenomenographic study was conducted with 10 university teachers, nine females and one male with more than 15 years of professional activity. The perception of the university emerges, in the teachers’ voice, focused on the description of its mission, namely as a context for the production and diffusion of knowledge to society, as a space for creative and critical thinking about the world, as an interdisciplinary space and as a system focused on teaching and research. It also includes characteristics related to its structure and functioning, such as the level of hierarchization, bureaucratization, competitiveness, dehumanization and bibliometrics overvaluation. Regarding the perceived changes, they are related to the structural reforms resulting from the Bologna Process, diverse student populations, research and internationalization, new technologies, institutional cooperation, bureaucratization and relationship with the community. Teachers also revealed some dissatisfaction in the way they are experiencing university life due to the overwork resulting from the multiple tasks required in the four activity strands (teaching, research, management and extension) with an impact on quality and innovation, but in line with what the institution demands.",book:{id:"10911",title:"Higher Education - New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg"},signatures:"Elisa Chaleta"},{id:"78595",title:"Globalization and Education: Trends towards Sustainability",slug:"globalization-and-education-trends-towards-sustainability",totalDownloads:58,totalDimensionsCites:0,doi:"10.5772/intechopen.99974",abstract:"Higher Education Institutions (IES) have a very relevant role in the path towards sustainability. The problem of the implementation of curricular sustainability is the disparity of solutions that can be adopted depending on the political and economic situation of each country. The study of a practical case in the south of Honduras allows the student to approach key decisions in a real scenario to bring improvements to a very disadvantaged population, lacking basic services, such as water and electricity, under the premise of sustainability, facing aspects as relevant such as sustainable mobility, water resources management, energy and construction models, in a context where globalization and technological innovation play a very important role. It is essential to know in depth the real context where structural changes will be applied to understand that there is no single reality, that actions are built adapting to specific situations and that the effectiveness of the measures that can be adopted to establish models that prioritize that part of sustainability that best weighs the balance between the environment, society and the economy for each case.",book:{id:"10911",title:"Higher Education - New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg"},signatures:"Maria Olga Bernaldo and Gonzalo Fernandez-Sanchez"},{id:"79255",title:"Higher Education Institutions (HEIs) in Africa Embracing the “New Normal” for Knowledge Production and Innovation: Barriers, Realities, and Possibilities",slug:"higher-education-institutions-heis-in-africa-embracing-the-new-normal-for-knowledge-production-and-i",totalDownloads:128,totalDimensionsCites:1,doi:"10.5772/intechopen.101063",abstract:"If Africa is to remain relevant and competitive in today’s knowledge-based economy, it has to rely on higher education institutions (HEIs) as centers of excellence for knowledge production. HEIs nurture and sustain the production of highly-skilled individuals to support Africa’s growing economies. Among all possible ways, this could be achievable through strategic curricula innovation driven by emerging mobile technologies. Consequently, Africa’s HEIs need to embrace the ‘New Normal’ by optimizing online teaching and learning in their pursuit to expand information and communications technology (ICT) literacy as a means to increase students’ opportunities in higher education (HE). However, Africa’s ability to embrace the ‘New Normal’ has been marred by inadequate ICT infrastructures, low connectivity, unreliable power supply, and national budget constraints that may undermine Africa’s HEIs’ potential to augment knowledge production and innovation.",book:{id:"10911",title:"Higher Education - New Approaches to Accreditation, Digitalization, and Globalization in the Age of Covid",coverURL:"https://cdn.intechopen.com/books/images_new/10911.jpg"},signatures:"Christopher B. 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The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. 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Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:3,paginationItems:[{id:"7",title:"Bioinformatics and Medical Informatics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/7.jpg",isOpenForSubmission:!0,editor:{id:"351533",title:"Dr.",name:"Slawomir",middleName:null,surname:"Wilczynski",slug:"slawomir-wilczynski",fullName:"Slawomir Wilczynski",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035U1loQAC/Profile_Picture_1630074514792",biography:"Professor Sławomir Wilczyński, Head of the Chair of Department of Basic Biomedical Sciences, Faculty of Pharmaceutical Sciences, Medical University of Silesia in Katowice, Poland. His research interests are focused on modern imaging methods used in medicine and pharmacy, including in particular hyperspectral imaging, dynamic thermovision analysis, high-resolution ultrasound, as well as other techniques such as EPR, NMR and hemispheric directional reflectance. Author of over 100 scientific works, patents and industrial designs. Expert of the Polish National Center for Research and Development, Member of the Investment Committee in the Bridge Alfa NCBiR program, expert of the Polish Ministry of Funds and Regional Policy, Polish Medical Research Agency. Editor-in-chief of the journal in the field of aesthetic medicine and dermatology - Aesthetica.",institutionString:null,institution:{name:"Medical University of Silesia",institutionURL:null,country:{name:"Poland"}}},editorTwo:null,editorThree:null},{id:"8",title:"Bioinspired Technology and Biomechanics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/8.jpg",isOpenForSubmission:!0,editor:{id:"144937",title:"Prof.",name:"Adriano",middleName:"De Oliveira",surname:"Andrade",slug:"adriano-andrade",fullName:"Adriano Andrade",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRC8QQAW/Profile_Picture_1625219101815",biography:"Dr. Adriano de Oliveira Andrade graduated in Electrical Engineering at the Federal University of Goiás (Brazil) in 1997. He received his MSc and PhD in Biomedical Engineering respectively from the Federal University of Uberlândia (UFU, Brazil) in 2000 and from the University of Reading (UK) in 2005. He completed a one-year Post-Doctoral Fellowship awarded by the DFAIT (Foreign Affairs and International Trade Canada) at the Institute of Biomedical Engineering of the University of New Brunswick (Canada) in 2010. Currently, he is Professor in the Faculty of Electrical Engineering (UFU). He has authored and co-authored more than 200 peer-reviewed publications in Biomedical Engineering. He has been a researcher of The National Council for Scientific and Technological Development (CNPq-Brazil) since 2009. He has served as an ad-hoc consultant for CNPq, CAPES (Coordination for the Improvement of Higher Education Personnel), FINEP (Brazilian Innovation Agency), and other funding bodies on several occasions. He was the Secretary of the Brazilian Society of Biomedical Engineering (SBEB) from 2015 to 2016, President of SBEB (2017-2018) and Vice-President of SBEB (2019-2020). He was the head of the undergraduate program in Biomedical Engineering of the Federal University of Uberlândia (2015 - June/2019) and the head of the Centre for Innovation and Technology Assessment in Health (NIATS/UFU) since 2010. He is the head of the Postgraduate Program in Biomedical Engineering (UFU, July/2019 - to date). He was the secretary of the Parkinson's Disease Association of Uberlândia (2018-2019). Dr. Andrade's primary area of research is focused towards getting information from the neuromuscular system to understand its strategies of organization, adaptation and controlling in the context of motor neuron diseases. 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He is also a faculty member in the Molecular Oncology Program. He obtained his MSc and Ph.D. at Oregon State University and Texas Tech University, respectively. He pursued his postdoctoral studies at Rutgers University Medical School and the National Institutes of Health (NIH/NIDDK), USA. His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"94311",title:"Prof.",name:"Martins",middleName:"Ochubiojo",surname:"Ochubiojo Emeje",slug:"martins-ochubiojo-emeje",fullName:"Martins Ochubiojo Emeje",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94311/images/system/94311.jpeg",biography:"Martins Emeje obtained a BPharm with distinction from Ahmadu Bello University, Nigeria, and an MPharm and Ph.D. from the University of Nigeria (UNN), where he received the best Ph.D. award and was enlisted as UNN’s “Face of Research.” He established the first nanomedicine center in Nigeria and was the pioneer head of the intellectual property and technology transfer as well as the technology innovation and support center. Prof. Emeje’s several international fellowships include the prestigious Raman fellowship. He has published more than 150 articles and patents. He is also the head of R&D at NIPRD and holds a visiting professor position at Nnamdi Azikiwe University, Nigeria. He has a postgraduate certificate in Project Management from Walden University, Minnesota, as well as a professional teaching certificate and a World Bank certification in Public Procurement. Prof. Emeje was a national chairman of academic pharmacists in Nigeria and the 2021 winner of the May & Baker Nigeria Plc–sponsored prize for professional service in research and innovation.",institutionString:"National Institute for Pharmaceutical Research and Development",institution:{name:"National Institute for Pharmaceutical Research and Development",country:{name:"Nigeria"}}},{id:"268659",title:"Ms.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/268659/images/8143_n.jpg",biography:"Dr. Zhan received his undergraduate and graduate training in the fields of preventive medicine and epidemiology and statistics at the West China University of Medical Sciences in China during 1989 to 1999. He received his post-doctoral training in oncology and cancer proteomics for two years at the Cancer Research Institute of Human Medical University in China. In 2001, he went to the University of Tennessee Health Science Center (UTHSC) in USA, where he was a post-doctoral researcher and focused on mass spectrometry and cancer proteomics. Then, he was appointed as an Assistant Professor of Neurology, UTHSC in 2005. He moved to the Cleveland Clinic in USA as a Project Scientist/Staff in 2006 where he focused on the studies of eye disease proteomics and biomarkers. He returned to UTHSC as an Assistant Professor of Neurology in the end of 2007, engaging in proteomics and biomarker studies of lung diseases and brain tumors, and initiating the studies of predictive, preventive, and personalized medicine (PPPM) in cancer. In 2010, he was promoted to Associate Professor of Neurology, UTHSC. Currently, he is a Professor at Xiangya Hospital of Central South University in China, Fellow of Royal Society of Medicine (FRSM), the European EPMA National Representative in China, Regular Member of American Association for the Advancement of Science (AAAS), European Cooperation of Science and Technology (e-COST) grant evaluator, Associate Editors of BMC Genomics, BMC Medical Genomics, EPMA Journal, and Frontiers in Endocrinology, Executive Editor-in-Chief of Med One. He has\npublished 116 peer-reviewed research articles, 16 book chapters, 2 books, and 2 US patents. His current main research interest focuses on the studies of cancer proteomics and biomarkers, and the use of modern omics techniques and systems biology for PPPM in cancer, and on the development and use of 2DE-LC/MS for the large-scale study of human proteoforms.",institutionString:null,institution:{name:"Xiangya Hospital Central South University",country:{name:"China"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"418340",title:"Dr.",name:"Jyotirmoi",middleName:null,surname:"Aich",slug:"jyotirmoi-aich",fullName:"Jyotirmoi Aich",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038Ugi5QAC/Profile_Picture_2022-04-15T07:48:28.png",biography:"Biotechnologist with 15 years of research including 6 years of teaching experience. Demonstrated record of scientific achievements through consistent publication record (H index = 13, with 874 citations) in high impact journals such as Nature Communications, Oncotarget, Annals of Oncology, PNAS, and AJRCCM, etc. Strong research professional with a post-doctorate from ACTREC where I gained experimental oncology experience in clinical settings and a doctorate from IGIB where I gained expertise in asthma pathophysiology. A well-trained biotechnologist with diverse experience on the bench across different research themes ranging from asthma to cancer and other infectious diseases. An individual with a strong commitment and innovative mindset. Have the ability to work on diverse projects such as regenerative and molecular medicine with an overall mindset of improving healthcare.",institutionString:"DY Patil Deemed to Be University",institution:null},{id:"349288",title:"Prof.",name:"Soumya",middleName:null,surname:"Basu",slug:"soumya-basu",fullName:"Soumya Basu",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035QxIDQA0/Profile_Picture_2022-04-15T07:47:01.jpg",biography:"Soumya Basu, Ph.D., is currently working as an Associate Professor at Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India. With 16+ years of trans-disciplinary research experience in Drug Design, development, and pre-clinical validation; 20+ research article publications in journals of repute, 9+ years of teaching experience, trained with cross-disciplinary education, Dr. Basu is a life-long learner and always thrives for new challenges.\r\nHer research area is the design and synthesis of small molecule partial agonists of PPAR-γ in lung cancer. She is also using artificial intelligence and deep learning methods to understand the exosomal miRNA’s role in cancer metastasis. Dr. Basu is the recipient of many awards including the Early Career Research Award from the Department of Science and Technology, Govt. of India. She is a reviewer of many journals like Molecular Biology Reports, Frontiers in Oncology, RSC Advances, PLOS ONE, Journal of Biomolecular Structure & Dynamics, Journal of Molecular Graphics and Modelling, etc. She has edited and authored/co-authored 21 journal papers, 3 book chapters, and 15 abstracts. She is a Board of Studies member at her university. She is a life member of 'The Cytometry Society”-in India and 'All India Cell Biology Society”- in India.",institutionString:"Dr. D.Y. Patil Vidyapeeth, Pune",institution:{name:"Dr. D.Y. Patil Vidyapeeth, Pune",country:{name:"India"}}},{id:"354817",title:"Dr.",name:"Anubhab",middleName:null,surname:"Mukherjee",slug:"anubhab-mukherjee",fullName:"Anubhab Mukherjee",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y0000365PbRQAU/ProfilePicture%202022-04-15%2005%3A11%3A18.480",biography:"A former member of Laboratory of Nanomedicine, Brigham and Women’s Hospital, Harvard University, Boston, USA, Dr. Anubhab Mukherjee is an ardent votary of science who strives to make an impact in the lives of those afflicted with cancer and other chronic/acute ailments. He completed his Ph.D. from CSIR-Indian Institute of Chemical Technology, Hyderabad, India, having been skilled with RNAi, liposomal drug delivery, preclinical cell and animal studies. He pursued post-doctoral research at College of Pharmacy, Health Science Center, Texas A & M University and was involved in another postdoctoral research at Department of Translational Neurosciences and Neurotherapeutics, John Wayne Cancer Institute, Santa Monica, California. In 2015, he worked in Harvard-MIT Health Sciences & Technology as a visiting scientist. He has substantial experience in nanotechnology-based formulation development and successfully served various Indian organizations to develop pharmaceuticals and nutraceutical products. He is an inventor in many US patents and an author in many peer-reviewed articles, book chapters and books published in various media of international repute. Dr. Mukherjee is currently serving as Principal Scientist, R&D at Esperer Onco Nutrition (EON) Pvt. Ltd. and heads the Hyderabad R&D center of the organization.",institutionString:"Esperer Onco Nutrition Pvt Ltd.",institution:null},{id:"319365",title:"Assistant Prof.",name:"Manash K.",middleName:null,surname:"Paul",slug:"manash-k.-paul",fullName:"Manash K. Paul",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/319365/images/system/319365.png",biography:"Manash K. Paul is a Principal Investigator and Scientist at the University of California Los Angeles. He has contributed significantly to the fields of stem cell biology, regenerative medicine, and lung cancer. His research focuses on various signaling processes involved in maintaining stem cell homeostasis during the injury-repair process, deciphering lung stem cell niche, pulmonary disease modeling, immuno-oncology, and drug discovery. He is currently investigating the role of extracellular vesicles in premalignant lung cell migration and detecting the metastatic phenotype of lung cancer via machine-learning-based analyses of exosomal signatures. Dr. Paul has published in more than fifty peer-reviewed international journals and is highly cited. He is the recipient of many awards, including the UCLA Vice Chancellor’s award, a senior member of the Institute of Electrical and Electronics Engineers (IEEE), and an editorial board member for several international journals.",institutionString:"University of California Los Angeles",institution:{name:"University of California Los Angeles",country:{name:"United States of America"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals. He is currently working on the protective activity of phenolic compounds in disorders associated with oxidative stress and inflammation.",institutionString:null,institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Dr.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. 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Fungal infectious illness prevalence and prognosis are determined by the exposure between fungi and host, host immunological state, fungal virulence, and early and accurate diagnosis and treatment. \r\nPatients with both congenital and acquired immunodeficiency are more likely to be infected with opportunistic mycosis. Fungal infectious disease outbreaks are common during the post- disaster rebuilding era, which is characterised by high population density, migration, and poor health and medical conditions.\r\nSystemic or local fungal infection is mainly associated with the fungi directly inhaled or inoculated in the environment during the disaster. The most common fungal infection pathways are human to human (anthropophilic), animal to human (zoophilic), and environment to human (soilophile). Diseases are common as a result of widespread exposure to pathogenic fungus dispersed into the environment. \r\nFungi that are both common and emerging are intertwined. In Southeast Asia, for example, Talaromyces marneffei is an important pathogenic thermally dimorphic fungus that causes systemic mycosis. Widespread fungal infections with complicated and variable clinical manifestations, such as Candida auris infection resistant to several antifungal medicines, Covid-19 associated with Trichoderma, and terbinafine resistant dermatophytosis in India, are among the most serious disorders. \r\nInappropriate local or systemic use of glucocorticoids, as well as their immunosuppressive effects, may lead to changes in fungal infection spectrum and clinical characteristics. Hematogenous candidiasis is a worrisome issue that affects people all over the world, particularly ICU patients. CARD9 deficiency and fungal infection have been major issues in recent years. Invasive aspergillosis is associated with a significant death rate. Special attention should be given to endemic fungal infections, identification of important clinical fungal infections advanced in yeasts, filamentous fungal infections, skin mycobiome and fungal genomes, and immunity to fungal infections.\r\nIn addition, endemic fungal diseases or uncommon fungal infections caused by Mucor irregularis, dermatophytosis, Malassezia, cryptococcosis, chromoblastomycosis, coccidiosis, blastomycosis, histoplasmosis, sporotrichosis, and other fungi, should be monitored. \r\nThis topic includes the research progress on the etiology and pathogenesis of fungal infections, new methods of isolation and identification, rapid detection, drug sensitivity testing, new antifungal drugs, schemes and case series reports. It will provide significant opportunities and support for scientists, clinical doctors, mycologists, antifungal drug researchers, public health practitioners, and epidemiologists from all over the world to share new research, ideas and solutions to promote the development and progress of medical mycology.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/4.jpg",keywords:"Emerging Fungal Pathogens, Invasive Infections, Epidemiology, Cell Membrane, Fungal Virulence, Diagnosis, Treatment"},{id:"5",title:"Parasitic Infectious Diseases",scope:"Parasitic diseases have evolved alongside their human hosts. In many cases, these diseases have adapted so well that they have developed efficient resilience methods in the human host and can live in the host for years. Others, particularly some blood parasites, can cause very acute diseases and are responsible for millions of deaths yearly. Many parasitic diseases are classified as neglected tropical diseases because they have received minimal funding over recent years and, in many cases, are under-reported despite the critical role they play in morbidity and mortality among human and animal hosts. The current topic, Parasitic Infectious Diseases, in the Infectious Diseases Series aims to publish studies on the systematics, epidemiology, molecular biology, genomics, pathogenesis, genetics, and clinical significance of parasitic diseases from blood borne to intestinal parasites as well as zoonotic parasites. We hope to cover all aspects of parasitic diseases to provide current and relevant research data on these very important diseases. In the current atmosphere of the Coronavirus pandemic, communities around the world, particularly those in different underdeveloped areas, are faced with the growing challenges of the high burden of parasitic diseases. At the same time, they are faced with the Covid-19 pandemic leading to what some authors have called potential syndemics that might worsen the outcome of such infections. Therefore, it is important to conduct studies that examine parasitic infections in the context of the coronavirus pandemic for the benefit of all communities to help foster more informed decisions for the betterment of human and animal health.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/5.jpg",keywords:"Blood Borne Parasites, Intestinal Parasites, Protozoa, Helminths, Arthropods, Water Born Parasites, Epidemiology, Molecular Biology, Systematics, Genomics, Proteomics, Ecology"},{id:"6",title:"Viral Infectious Diseases",scope:"The Viral Infectious Diseases Book Series aims to provide a comprehensive overview of recent research trends and discoveries in various viral infectious diseases emerging around the globe. The emergence of any viral disease is hard to anticipate, which often contributes to death. A viral disease can be defined as an infectious disease that has recently appeared within a population or exists in nature with the rapid expansion of incident or geographic range. This series will focus on various crucial factors related to emerging viral infectious diseases, including epidemiology, pathogenesis, host immune response, clinical manifestations, diagnosis, treatment, and clinical recommendations for managing viral infectious diseases, highlighting the recent issues with future directions for effective therapeutic strategies.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/6.jpg",keywords:"Novel Viruses, Virus Transmission, Virus Evolution, Molecular Virology, Control and Prevention, Virus-host Interaction"}],annualVolumeBook:{},thematicCollection:[{type:"book",id:"11672",title:"Chemokines Updates",subtitle:null,isOpenForSubmission:!0,hash:"c00855833476a514d37abf7c846e16e9",slug:null,bookSignature:"Prof. Murat Şentürk",coverURL:"https://cdn.intechopen.com/books/images_new/11672.jpg",editedByType:null,submissionDeadline:"May 6th 2022",editors:[{id:"14794",title:"Prof.",name:"Murat",middleName:null,surname:"Şentürk",slug:"murat-senturk",fullName:"Murat Şentürk",profilePictureURL:"https://mts.intechopen.com/storage/users/14794/images/system/14794.jpeg",biography:"Dr. Murat Şentürk obtained a baccalaureate degree in Chemistry in 2002, a master’s degree in Biochemistry in 2006, and a doctorate degree in Biochemistry in 2009 from Atatürk University, Turkey. Dr. Şentürk currently works as an professor of Biochemistry in the Department of Basic Pharmacy Sciences, Faculty of Pharmacy, Ağri Ibrahim Cecen University, Turkey. \nDr. Şentürk published over 120 scientific papers, reviews, and book chapters and presented several conferences to scientists. \nHis research interests span enzyme inhibitor or activator, protein expression, purification and characterization, drug design and synthesis, toxicology, and pharmacology. \nHis research work has focused on neurodegenerative diseases and cancer treatment. Dr. Şentürk serves as the editorial board member of several international journals.",institutionString:"Ağrı İbrahim Çeçen University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"2",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"Ağrı İbrahim Çeçen University",institutionURL:null,country:{name:"Turkey"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}],selectedSeries:{title:"Infectious Diseases",id:"6"},selectedSubseries:null},seriesLanding:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfOpenTopics:4,numberOfPublishedChapters:318,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},subseries:[{id:"14",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. Topics include, but are not limited to: Advanced techniques of cellular and molecular biology (Molecular methodologies, imaging techniques, and bioinformatics); Biological activities at the molecular level; Biological processes of cell functions, cell division, senescence, maintenance, and cell death; Biomolecules interactions; Cancer; Cell biology; Chemical biology; Computational biology; Cytochemistry; Developmental biology; Disease mechanisms and therapeutics; DNA, and RNA metabolism; Gene functions, genetics, and genomics; Genetics; Immunology; Medical microbiology; Molecular biology; Molecular genetics; Molecular processes of cell and organelle dynamics; Neuroscience; Protein biosynthesis, degradation, and functions; Regulation of molecular interactions in a cell; Signalling networks and system biology; Structural biology; Virology and microbiology.",annualVolume:11410,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"79367",title:"Dr.",name:"Ana Isabel",middleName:null,surname:"Flores",fullName:"Ana Isabel Flores",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRpIOQA0/Profile_Picture_1632418099564",institutionString:null,institution:{name:"Hospital Universitario 12 De Octubre",institutionURL:null,country:{name:"Spain"}}},{id:"328234",title:"Ph.D.",name:"Christian",middleName:null,surname:"Palavecino",fullName:"Christian Palavecino",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000030DhEhQAK/Profile_Picture_1628835318625",institutionString:null,institution:{name:"Central University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"186585",title:"Dr.",name:"Francisco Javier",middleName:null,surname:"Martin-Romero",fullName:"Francisco Javier Martin-Romero",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB3HQAW/Profile_Picture_1631258137641",institutionString:null,institution:{name:"University of Extremadura",institutionURL:null,country:{name:"Spain"}}}]},{id:"15",title:"Chemical Biology",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors",scope:"Chemical biology spans the fields of chemistry and biology involving the application of biological and chemical molecules and techniques. In recent years, the application of chemistry to biological molecules has gained significant interest in medicinal and pharmacological studies. This topic will be devoted to understanding the interplay between biomolecules and chemical compounds, their structure and function, and their potential applications in related fields. Being a part of the biochemistry discipline, the ideas and concepts that have emerged from Chemical Biology have affected other related areas. This topic will closely deal with all emerging trends in this discipline.",annualVolume:11411,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null,editorialBoard:[{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",fullName:"Abdulsamed Kükürt",profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",institutionString:null,institution:{name:"Kafkas University",institutionURL:null,country:{name:"Turkey"}}},{id:"241413",title:"Dr.",name:"Azhar",middleName:null,surname:"Rasul",fullName:"Azhar Rasul",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRT1oQAG/Profile_Picture_1635251978933",institutionString:null,institution:{name:"Government College University, Faisalabad",institutionURL:null,country:{name:"Pakistan"}}},{id:"178316",title:"Ph.D.",name:"Sergey",middleName:null,surname:"Sedykh",fullName:"Sergey Sedykh",profilePictureURL:"https://mts.intechopen.com/storage/users/178316/images/system/178316.jfif",institutionString:null,institution:{name:"Novosibirsk State University",institutionURL:null,country:{name:"Russia"}}}]},{id:"17",title:"Metabolism",keywords:"Biomolecules Metabolism, Energy Metabolism, Metabolic Pathways, Key Metabolic Enzymes, Metabolic Adaptation",scope:"Metabolism is frequently defined in biochemistry textbooks as the overall process that allows living systems to acquire and use the free energy they need for their vital functions or the chemical processes that occur within a living organism to maintain life. Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. Thus all studies on metabolism will be considered for publication.",annualVolume:11413,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null,editorialBoard:[{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",fullName:"Anca Pantea Stoian",profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",institutionURL:null,country:{name:"Romania"}}},{id:"203824",title:"Dr.",name:"Attilio",middleName:null,surname:"Rigotti",fullName:"Attilio Rigotti",profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institutionString:null,institution:{name:"Pontifical Catholic University of Chile",institutionURL:null,country:{name:"Chile"}}},{id:"300470",title:"Dr.",name:"Yanfei (Jacob)",middleName:null,surname:"Qi",fullName:"Yanfei (Jacob) Qi",profilePictureURL:"https://mts.intechopen.com/storage/users/300470/images/system/300470.jpg",institutionString:null,institution:{name:"Centenary Institute of Cancer Medicine and Cell Biology",institutionURL:null,country:{name:"Australia"}}}]},{id:"18",title:"Proteomics",keywords:"Mono- and Two-Dimensional Gel Electrophoresis (1-and 2-DE), Liquid Chromatography (LC), Mass Spectrometry/Tandem Mass Spectrometry (MS; MS/MS), Proteins",scope:"With the recognition that the human genome cannot provide answers to the etiology of a disorder, changes in the proteins expressed by a genome became a focus in research. Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. The Proteomics topic aims to attract contributions on all aspects of MS-based proteomics that, by pushing the boundaries of MS capabilities, may address biological problems that have not been resolved yet.",annualVolume:11414,isOpenForSubmission:!0,coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null,editorialBoard:[{id:"72288",title:"Dr.",name:"Arli Aditya",middleName:null,surname:"Parikesit",fullName:"Arli Aditya Parikesit",profilePictureURL:"https://mts.intechopen.com/storage/users/72288/images/system/72288.jpg",institutionString:null,institution:{name:"Indonesia International Institute for Life Sciences",institutionURL:null,country:{name:"Indonesia"}}},{id:"40928",title:"Dr.",name:"Cesar",middleName:null,surname:"Lopez-Camarillo",fullName:"Cesar Lopez-Camarillo",profilePictureURL:"https://mts.intechopen.com/storage/users/40928/images/3884_n.png",institutionString:null,institution:{name:"Universidad Autónoma de la Ciudad de México",institutionURL:null,country:{name:"Mexico"}}},{id:"81926",title:"Dr.",name:"Shymaa",middleName:null,surname:"Enany",fullName:"Shymaa Enany",profilePictureURL:"https://mts.intechopen.com/storage/users/81926/images/system/81926.png",institutionString:"Suez Canal University",institution:{name:"Suez Canal University",institutionURL:null,country:{name:"Egypt"}}}]}]}},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"profile.detail",path:"/profiles/192064",hash:"",query:{},params:{id:"192064"},fullPath:"/profiles/192064",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var e;(e=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(e)}()