Chapters authored
The Utilization of Translocation Lines and Microsatellite Markers for the Identification of Unknown Cotton Monosomic Lines
Simple sequence repeats (SSR) have been applied as useful markers for understanding cotton genetics. In the last decade, chromosome-deficient stocks of Gossypium hirsutum L. were used in the development of chromosome substitution lines for G. barbadense L., G. tomentosum Nuttall et Seemann, and G. mustelinum Watt chromosomes or chromosome segments. Several DNA markers have already been assigned to the individual chromosomes of G. hirsutum. We created new cotton monosomic lines in Uzbekistan after irradiation of seeds by thermal neutrons or pollen gamma‐irradiation to complement other global efforts in the development of cotton chromosome substitution lines. The primary objective of this chapter is to report the use of chromosome-specific SSR markers and a well-defined tester set of cotton translocation lines from the Cotton Cytogenetic Collection at Texas A&M AgriLife Research to confirm chromosome specificity of monosomic lines in Uzbekistan cytogenetic collection of cotton. Our results have assigned several different monosomic lines to the chromosomes 2, 4, 6, and telosome 11 At‐subgenome and chromosomes 18 and 20 or 22 Dt‐subgenome. These lines will be very useful in molecular mapping, the creation of substitution lines, and cotton breeding.
Part of the book: Cotton Research
Recent Studies on Fusarium Wilt in Cotton
Fusarium oxysporum has been a subject of intensive research since 1882, with over 200 studies published from key cotton-growing countries such as the United States, China, Uzbekistan, India, Pakistan, Australia, and Brazil. The present study has employed a diverse array of research methodologies and technological approaches, primarily emphasizing research publications disseminated within the past decade. It places specific emphasis on two key domains: Molecular Mapping and Genome-Wide Association Studies (GWAS), elucidating the evolutionary analysis transition from Simple Sequence Repeat (SSR) to Single Nucleotide Polymorphism (SNP) chip utilization. The creation of a comprehensive molecular map that incorporates Quantitative Trait Loci (QTLs) related to Fusarium and consolidates findings from several research groups, accompanied by figures and tables, serves to facilitate a more thorough understanding of the genetic architecture underlying Fusarium-related traits. An in-depth examination of recent advances in marker-assisted selection for traits conferring resistance to Fusarium oxysporum f. sp. vasinfectum (FOV), coupled with a comprehensive evaluation of the pertinent genes, offers valuable insights into the development of resistant cultivars and the underlying genetic mechanisms. This entails doing a critical review of recent relevant literature. Furthermore, this investigation examines the obstacles and potential associated with developing technologies.
Part of the book: Fusarium - Recent Studies [Working title]