We demonstrated the value of multiparameter flowcytometry in detecting human tumor cells of breast cancer in peripheral blood, which had a sensitivity limit of 10-5 and higher specificity compares with real‐time polymerase chain reaction (RT‐PCR). It was also found that circulating tumor cell (CTC) number was related with TNM stage, metastasis and the overall survival of patients. CTC level was one of the important factors for patients’ prognosis. At the same time, we also verified the circulating tumor stem cell (CTSC) was connected with TNM stage by multiparameter cytometry. The detection of CTC and CTSC by multiparameter flowcytometry may be used to diagnose disease at early stage to guide clinical therapy or to predict prognosis. Multiparameter flowcytometry has the potential to be a valuable tool for prognosis assessment among patients with breast cancer in clinical situation in China.
Part of the book: Tumor Metastasis