Glyphosate is a pesticide widely used in agriculture, horticulture, and silviculture as well as around homes and gardens. It was introduced by Monsanto in the early 1970s, and it is a broad spectrum, nonselective, post-emergence herbicide that inhibits plants’ shikimic acid pathway. Glyphosate is considered as “difficult herbicide” in terms of trace analysis. It has low molecular weight, low volatility, thermal lability, and good water solubility. These properties cause problems in its extraction, purification, and detection. The determination often requires additional processes that may allow quantification by chromatographic methods. Several analytical procedures have been developed based on solid-phase extraction, ion-exchange chromatography, or matrix solid phase dispersion. Most published methods involve liquid extraction followed by clean-up. This review would like to revise the literature on this issue discussing the relevant chromatographic methods reported in the literature in terms of analytical parameters for analyzing such compound in food chain.