Fibrinogen (Fbg) concentration values in different vertebrates
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Barely three months into the new year and we are happy to announce a monumental milestone reached - 150 million downloads.
\n\nThis achievement solidifies IntechOpen’s place as a pioneer in Open Access publishing and the home to some of the most relevant scientific research available through Open Access.
\n\nWe are so proud to have worked with so many bright minds throughout the years who have helped us spread knowledge through the power of Open Access and we look forward to continuing to support some of the greatest thinkers of our day.
\n\nThank you for making IntechOpen your place of learning, sharing, and discovery, and here’s to 150 million more!
\n\n\n\n\n'}],latestNews:[{slug:"intechopen-supports-asapbio-s-new-initiative-publish-your-reviews-20220729",title:"IntechOpen Supports ASAPbio’s New Initiative Publish Your Reviews"},{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"}]},book:{item:{type:"book",id:"539",leadTitle:null,fullTitle:"Novel Aspects in Acute Lymphoblastic Leukemia",title:"Novel Aspects in Acute Lymphoblastic Leukemia",subtitle:null,reviewType:"peer-reviewed",abstract:"Acute lymphoblastic leukemia (ALL) has turned from a universally fatal to a highly curable disease in little more than four decades. Even though differences in outcome continue to exist between children and adults, intense efforts are under way to overcome this discrepancy and improve the prognosis of adult patients as well. This exemplary progress in ALL therapy has been possible by the combination of an increasingly better understanding of the biology of the disease, availability of a range of effective drugs, and astute designs and relentless executions of many clinical trials. ALL is a complex disease requiring complex therapy. Whereas this book cannot provide a comprehensive review of every one of its many facets, the chapters from many investigators from around the world nevertheless cover a number of relevant topics: aspects of the epidemiology of ALL in Hispanics, ophthalmologic manifestations of ALL, overviews of current therapy and drug-resistance mechanisms, novel biological pathways and targets, new drugs in development, and long-term consequences of CNS prophylaxis and therapy. The publishers and editor therefore hope that the prospective readers will find enough insight and information for their own endeavors.",isbn:null,printIsbn:"978-953-307-753-6",pdfIsbn:"978-953-51-6573-6",doi:"10.5772/1050",price:119,priceEur:129,priceUsd:155,slug:"novel-aspects-in-acute-lymphoblastic-leukemia",numberOfPages:272,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"dfef11575616931bbc329551f943115f",bookSignature:"Stefan Faderl",publishedDate:"November 16th 2011",coverURL:"https://cdn.intechopen.com/books/images_new/539.jpg",numberOfDownloads:35712,numberOfWosCitations:17,numberOfCrossrefCitations:7,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:23,numberOfDimensionsCitationsByBook:0,hasAltmetrics:0,numberOfTotalCitations:47,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"February 1st 2011",dateEndSecondStepPublish:"March 1st 2011",dateEndThirdStepPublish:"July 6th 2011",dateEndFourthStepPublish:"August 5th 2011",dateEndFifthStepPublish:"December 3rd 2011",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"64603",title:"Dr.",name:"Stefan",middleName:null,surname:"Faderl",slug:"stefan-faderl",fullName:"Stefan Faderl",profilePictureURL:"https://mts.intechopen.com/storage/users/64603/images/1876_n.jpg",biography:"Stefan Faderl, M.D., Professor of Medicine came to M.D. Anderson in 1996. Dr. Faderl graduated from Ludwig Maximilian Medical School in Munich, Germany in 1990 and received his medical/academic degree (magna cum laude) from the same institution in 1994. Dr. Faderl is board certified in Internal Medicine and Medical Oncology. He specializes in acute and chronic leukemias. His main areas of interest include acute lymphoblastic leukemias (ALL), acute myeloid leukemias (AML), chronic lymphocytic leukemia (CLL) and its variants, as well as chronic myeloid leukemias (CML). Dr. Faderl has authored and co-authored many abstracts, articles in peer-reviewed journals and book chapters. 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Coagulation factors circulate in the blood as proenzymes until they are activated by vascular damage (Lane et al. 2005; Owens and Mackman 2010). These enzymes amplified and disseminated the sequence and then are stopped by natural inhibitors (Mulder et al. 2010; Middeldorp 2011) and the fibrinolytic system (Greenberg and Orthner 1999; Levi et al. 2012). Cellular phospholipids make the process much more efficient (Hoffman 2003; Gentry 2004; Rivera et al. 2009).Activated Factor XIIIa stabilizes the polymer (Sidelmann et al. 2000; Greenberg and Lai 2003; Muszbek et al. 2011). Plasminogen (Plg) is the key in thrombus lysis; and is synthesized in mammals principally by the liver (Stafford 1964; Degen 2001; Zhang et al. 2002; Zorio et al. 2008). Natural Plg activators are: tissue plasminogen activator (tPA) and urokinase (uPA) (Fleming and Melzig 2012); streptokinase (SK) acts as in an exogenous path (Sazonova et al. 2009). Free Plm is very active and degrades other proteins, such as complement, fibrinogen (Fbg), factors II, V and VIII or activates metallo-proteases involved in tissue remodeling by degradation of cellular matrix (Collen 2001; Parfyonova et al. 2002; Dewyer et al. 2007).The main inhibitors of Plm are the alpha2 plasmin inhibitor (α2PI) (Menoud et al. 1996; Fraser et al. 2011) and Plasminogen activator inhibitor type 1 (PAI-1) (Declerk et al. 1998; Vaughan 2005). Thrombin activatable fibrinolysis inhibitor (TAFI) is a link between the two systems, it is activated by thrombin generated during coagulation, and suppresses fibrinolysis (Marx 2004; Hilmayer et al. 2006; Milijic et al. 2010).
There is a growing homology in the components of the fibrinolytic system along zoological evolution. Fibrinolysis is present in all vertebrates but invertebrates generally only have clumping of blood corpuscles (Withers 1992). Vertebrates factors involved in coagulation and fibrinolysis have evolved from common ancestral proteins and fibrinolytic ones seem to be related to digestive proteolytic enzymes used by rudimentary microorganisms to be released and disseminated, avoiding the host´s nonspecific defense and immunity response (Patthy 1990; Gladysheva et al, 2003; Opal and Esmon 2003; Levi et al. 2012).
Insects have rich sources of pharmacological active substances that may have medical value: The venom of
Very little is known about the fibrinolytic system and its component concentrations in animals and the relevance of these models for human health is questioned due to many reasons: interspecies differences (Siller-Matula et al. 2008; Ralph and Brainard 2012), lack of reliable results (Vap et al. 2012), use of diagnostic equipment designed only for human care, inadequate relationship of test reagent to clotting factor concentration (Ravanat et al. 1995; Jagadeeswaran and Sheehan 1999; Kubalek et al. 2002, Münster et al. 2002; Gentry 2004; Weir-M et al. 2004). Also, anatomical features of the animal chosen can make it really difficult to obtain good quality blood samples (Saito et al. 1976; Meinkoth and Allison 2007). For example, vessel size and blood flow are important determinants of vascular function when mouse model is used for human research of aorta (Fay et al 2007).
In this chapter we summarize the actual knowledge about fibrinolytic assays among different animal species and we compare these findings with healthy adult human beings.
A review of laboratory tests was conducted in a phylogenetic order: fish, amphibians, reptiles, birds and mammals. It was designed to assess the fibrinolytic system in its various stages: global (whole blood lysis time WBLT, whole blood diluted lysis time WDLT, euglobulin lysis time ELT), specific (Plg, PAI-1, tPA, α2PI and the thrombin-activatable fibrinolysis inhibitor TAFI) and degradation products generated from the degradation of fibrinogen / fibrin FDP, D Dimer DD, and Plm-α2PI, tPA-PAI-1, uPA-PAI-1 complexes (Blanco 2003; Urano and Suzuki 2011).
The results of these assays are summarized in Tables 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 (Tentoni et al, 2010).
In fishes the information is insufficient (Tables 1 and 2). WBLT is undetectable in lamprey and black fish, while lysis is fast in dog fish. The genes encoded for Plg and tPA were identified in the blowfish
In amphibians (Tables 1 to 3), the marine toad
There is no evidence of a fibrinolytic system in reptiles, lizards (Trachydosaurus rugosus rugosus, Tiliqua scincoides, Amphibolorus barbatus, Varanus acanthrus, Iguana iguana), turtles (Chelodina longicollis), crocodiles (Crocodylus porosus) or pitons (Liasis spp, Morelia spp) (Tables 1 and 10). A strong circulating antithrombin protects these vertebrates from intravascular thrombosis (Hackett and Hann 1964; Kubalek et al. 2002), however low concentrations of α2PI were detected in the circulation of the snake Bitis arietans using a chromogenic method (Table 10).
Snake venoms are mixtures of many peptides which affect the blood coagulation and fibrinolysis pathways such as Plg activators (Kini 2005; Miller et al 2009) and fibrinogen degradators (Meyer 2000). Recently a non hemorrhagic metalloproteinase (BleucMP) was purified from
Birds are deficient in Factors XI and XII so the clotting times exceeding 70 minutes (Wartelle 1957; Soulier et al. 1959, Bigland 1964). Fibrinolysis can be activated with the saliva of the vampire
The WDLT in the
FDP was undetectable in the
Plg activators similar to tPA were discovered in the South American vampire bat´s
In dogs (Tables 1, 3, 4, 5, 6, 7 and 10), except for the Plg when it is measured by activation with SK, the values of all the fibrinolytic assays are quite similar to the values in humans, as reported by Wohl et al. (1983).
In cats (Tables 1, 3, 5, 9 and 10) there is a marked difference in functional PAI-1 activity when compared to man, and its Plg cannot be activated with tPA but with uPA (Welles 1996).
In studied rodents, the fibrinolytic system is quite similar to that in humans, but Plg is poorly activated with SK; Plg, tPA, uPA and PAI-1 have been described in
Rat (Tables 1, 3, 4, 5, 7, 8, 9 and 10), guinea pigs (Tables 4, 5 and 10) and rabbits (Tables 1, 3, 4, 5, 7, 9 y 10) are the most employed animal models in fibrinolytic research.
Plg cannot be activated with SK in cattle (Tables 1, 5, 6 and 10), pigs (Tables 1, 5, 7 and 10) and sheep (Tables 5, 7 and 10), (Cliffton and Cannamella 1953; Korninger and Colleen 1981; Wohl et al 1983; Zhang et al 2012). Horses (Tables 1, 5, 6, 7, 9 and 10) have higher levels of functional PAI-1 and α2PI when compared to humans (Barton et al. 1998). The fibrinolytic activity in llama is similar to that of horses and other domestic species (Morin et al 1995).
In armadillos
\n | \n \n | \n \n | \n
human | \n188 - 381 | \nWilliams | \n
armadilloa | \n211 - 333 | \nCasanave | \n
armadilloa´ | \n258 - 380 | \nTentoni | \n
whaleb | \n147 | \nSaito | \n
iguanac | \n420 - 440 | \nKubalek | \n
catd | \n50 - 165 | \nO´Rourke | \n
cat | \n150 - 400 | \nHerring and McMichael, 2012 | \n
eaglee | \n80 - 160 | \nGarcía-Montijano | \n
frogf | \n590 - 990 | \nCoppo | \n
dolphing | \n269 - 417 | \nTibbs | \n
mouseh | \n200 - 260 | \nTsakiris | \n
dog | \n141 - 227 | \nMischke | \n
dog | \n179. - 329 | \nMachida | \n
dog | \n150 - 400 | \nHerring and McMichael, 2012 | \n
rat | \n168 - 192 | \nHonda | \n
japanese quaili | \n140 - 260 | \nBelleville | \n
pigj | \n181 - 534 | \nVelik-Salchner | \n
pig | \n130 - 170 | \nSchöchl | \n
rabbitk | \n257 - 286 | \nMarval | \n
cowl | \n125 - 697 | \nHeuwieser | \n
sheep | \n178 - 215 | \nWilhelmi | \n
horsem | \n78 - 156 | \nBarton | \n
monkeyn | \n119 - 239 | \nSuzuki | \n
elephant sealo | \n50 - 162 | \nGulland | \n
capybarap | \n124 | \nLeitâo | \n
ostrichq | \n172 - 356 | \nFrost | \n
caimanr | \n430 - 1500 | \nArocha-Piñango | \n
marine fishs | \n220 - 280 | \nPavlidis | \n
asian elephantt | \n412 - 510 | \nGentry | \n
vultureu | \n< 20 | \nWeir-M | \n
llamav | \n140 - 300 | \nMorin | \n
Fibrinogen (Fbg) concentration values in different vertebrates
\n | \n \n | \n \n | \n
human | \n"/> 24 | \nConard, 1976 | \n
lampreya | \nnd | \nHawkey, 1971 | \n
\n | \n nd | \nHawkey, 1971 | \n
\n | \n nd | \nBlofield, 1965 | \n
\n | \n nd | \n|
\n | \n nd | \n|
domestic birds | \nnd | \nNiewiarowski & Latallo, 1959 | \n
dogfishf | \n2 – 4 | \nHawkey, 1971 Doolittle & Surgernor, 1962 | \n
japanese quailg | \n"/> 72 | \nBelleville | \n
armadillo | \n"/> 72 | \nTentoni | \n
Whole blood lysis time (WBLT) values in different vertebrates
a Petromyzon marinus; b Tautoga onitis; c Rana temporaria; d Rana pipiens; e Xenopus lavéis; f Mustelus canis; g Coturnix coturnix japonica (n:10 adult males); nd: not detectable; > more than.
\n | \n \n | \n \n | \n
human | \n> 20 | \nFearnley | \n
tiger froga | \n> 48 | \nSrivastava | \n
\n | \n 5.9 – 8.5 | \nLohman | \n
dogc | \n> 20 | \nHedlin | \n
ratd | \n> 20 | \n|
rabbite | \n> 20 | \n|
rabbitf | \n> 30 | \nHassett | \n
catg | \nnd | \nWelles | \n
armadillo | \n> 72 | \nTentoni | \n
Whole blood diluted lysis time (WDLT) values in different vertebrates
nd: not detectable; a
\n | \n \n | \n \n | \n
human | \n"/> 120 | \nKowalski | \n
armadilloa | \n15.4 – 45.6 | \nBermúdez, 2003 | \n
armadillo a´ | \n24.5 - 93 | \nTentoni | \n
\n | \n nd | \nSrivastava | \n
japanese quailc | \nnd | \nBelleville | \n
dog | \n21 - 109 | \nHedlin | \n
\n | \n < 90 | \nKaspareit | \n
rabbit | \n270 - 450 | \nHassett | \n
\n | \n 240 | \nSuzuki et al, 1977 | \n
\n | \n nd | \nWeir-M | \n
\n | \n 105 - 145 | \nGroza | \n
Euglobulin lysis time (ELT) values in different vertebrates
a
\n | \n \n | \n \n | \n
human | \n80 - 120 | \nPerkins, 1999 | \n
japanese quaila | \n0 | \nBelleville | \n
dog | \n102 - 115 # | \nLanevschi | \n
dog | \n3,2 - 4,4 | \nKarges | \n
cat | \n50 - 200 | \nO´Rourke | \n
cat | \n94 - 122 | \nKarges | \n
rat | \n6 - 14 | \n|
guinea pigb | \n0.4 – 6.1 | \n|
rabbit | \n2 | \n|
rabbit | \n147 - 217 # | \nMarval | \n
rabbit | \n84 - 108 # | \nHassett | \n
sheep | \n0.7 – 1.5 | \nKarges | \n
cow | \n0 | \n|
monkeyc | \n24 - 39 | \n|
monkeyd | \n164 # | \nSuzuki | \n
capybarae | \n0 | \nLeitâo | \n
pig | \n2.1 – 5.2 | \nKarges | \n
pig | \n0 | \nHahn | \n
horsef | \n66.5 – 98.1 | \nBarton | \n
whaleg | \n112 # | \nSaito | \n
armadillo | \n28 - 40 | \nTentoni | \n
Plasminogen (Plg) activity values in different vertebrates
Results are expressed as percent for Plg activity in relation to the calibration curve obtained with a pool of healthy humans platelets poor plasma, using a chromogenix assay after activation with SK.
a
\n | \n \n | \n \n | \n
human | \n< 10 | \nAmiral | \n
\n | \n < 5 | \nBoisvert | \n
\n | \n < 5 | \nStokol, 2003 | \n
dog | \n< 5 | \nGriffin | \n
dog | \n0 – 1.18 | \nMachida | \n
dog | \n< 10 | \nHerring & McMichael, 2012 | \n
cat | \n< 10 | \nHerring & McMichael, 2012 | \n
horse | \n5.5 – 10.9 | \nBarton | \n
horse | \n< 10 | \nStokol | \n
elephant seala | \n0 | \nGulland | \n
dolphinb | \n< 10 | \nTibbs | \n
cow | \n< 5 | \nIrmak & Turgut, 2005 | \n
armadillo | \n0 - 10 | \nTentoni | \n
Fibrin fibrinogen degradation products (FDP) concentration values in different mamals
A
\n | \n \n | \n \n | \n
human | \n< 0.50 | \nEstève | \n
dog | \n0.08 – 0.39 | \nStokol | \n
dog | \n0.02 – 0.28 | \nCaldin | \n
dog | \n< 0.25 | \nNelson, 2005 | \n
dog | \n< 0.25 | \nHerring & McMichael, 2012 | \n
cat | \n< 0.25 | \nHerring & McMichael, 2012 | \n
rat | \n0.18 | \nAsakura | \n
rat | \n< 0.02 | \nRavanant | \n
hen | \n< 0.02 | \n|
rabbit | \n< 0.02 | \n|
sheep | \n< 0.02 | \n|
monkeya | \n< 0.05 | \n|
mouse | \n< 0.02 | \n|
mouse | \n0 | \nTsakiris | \n
pig | \n0 | \nRoussi | \n
pig | \n< 0.01 | \nSchöchl | \n
horseb | \n0.46 – 0.92 | \nMonreal | \n
horse | \n0 – 0.91 | \nMachida | \n
horse | \n< 0.50 | \nStokol | \n
ostrich | \n0.25 | \nFrost | \n
vulture | \n"/> 1 | \nWeir-M | \n
armadillo | \nnd | \nTentoni | \n
dolphin | \n< 0.50 | \nTibbs | \n
D Dimer (DD) concentration values in different vertebrates.
A
\n | \n \n | \n \n | \n
human | \n4 – 43 | \nDeclerck | \n
\n | \n 1.3 – 2.5 | \nTsakiris | \n
\n | \n 1 – 2 | \nMatsuo | \n
\n | \n 0 | \nRoussi | \n
\n | \n 5.6 – 9.0 | \nSchöchl | \n
\n | \n 1.0 – 2.2 | \nTentoni | \n
\n | \n 3.9 | \nNieuwenhuys | \n
Immunological Plasminogen activator inhibitor type 1 (PAI-1) concentration in different mammals
A
\n | \n \n | \n |
human | \n< 10 | \nVan Cott & Laposata, 2001 | \n
\n | \n 0 | \nWelles, 1996 | \n
\n | \n 0.06 – 0.16 | \nHassett | \n
horse | \n19.6 – 42.2 | \nBarton | \n
armadillo | \n24.8 – 37.7 | \nTentoni | \n
rat | \n1.0 | \nNobukata | \n
rat | \n4.9 – 7.4 | \nEmeis | \n
Functional Plasminogen activator inhibitor type 1 (PAI-1) concentration in different mammals
Results are expressed as units of PAI-1 present in plasma in relation to the calibration curve obtained with a commercial standard when using immunological test; < less than
\n | \n \n | \n \n | \n
Human | \n70 - 130 | \nTeger-Nilsson | \n
\n | \n 65 - 85 | \nBelleville | \n
\n | \n 115.6 | \nFrost | \n
hen | \n109.4 | \n|
snakec | \n10 | \n|
\n | \n 68.8 | \n|
whaled | \n50 | \nSaito | \n
\n | \n 96 - 103 | \nLanevschi | \n
\n | \n 92 - 94 | \nKarges | \n
\n | \n 70 - 86 | \n|
\n | \n 118 - 138 | \n|
\n | \n 91 - 101 | \n|
\n | \n 90 - 109 | \n|
\n | \n 63 - 104 | \n|
\n | \n 82 - 99 | \n|
\n | \n 91 - 108 | \n|
\n | \n 66 - 92 | \nHassett | \n
\n | \n 87 - 127 | \nHahn | \n
\n | \n 120 | \nNobukata | \n
\n | \n 154 - 240 | \nBarton | \n
\n | \n 80 - 94 | \nDaugschies | \n
\n | \n 72 - 101 | \nTentoni | \n
alpha2 plasmin inhibitor activity (α2PI) in different vertebrates
Results are expressed as percent for α2PI activity in relation to the calibration curve obtained with a pool of healthy humans platelets poor plasma, using a chromogenix assay after activation with an excess of Plm.
The information summarized in this chapter helps the choice of appropriate animal experimental models for studying fibrinolysis and the correct extrapolation of animal results toward humans. Previous work from our laboratory, has identified the choice of the armadillo as an animal model because it adapts well to captivity conditions, endures repeated blood sampling, shows excellent tolerance to cardiac puncture and recovers quickly from anaesthesia (Bermúdez et al. 2004; Casanave et al. 2005; 2006).
This work was supported by Secretaría General de Ciencia y Tecnología, Universidad Nacional del Sur (SGCyT-UNS), Project 24/B152 and by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), PICTR 74/02, Argentina.
There are various bacterial, fungal, viral, and other microscopic life forms in our environment. Microorganisms make up 80–90% of the earth’s total biomass, and even under “clean” conditions, several thousand fungal spores can be inhaled per day. Many microorganisms are harmless or even beneficial, but others can be extremely dangerous or even deadly. The current way of life creates favorable conditions for the spread of infections (food from distant lands, work in air-conditioned rooms, frequent trips to foreign countries, visits to hospitals, etc.). The human body is not sterile, and it is colonized by many microorganisms that are part of the normal microflora and live like harmless commandants.
Microorganisms living under normal conditions on the skin, nasopharynx, and intestine play an important protective role, as they prevent the growth of pathogenic microorganisms in these places. As the bodies condition changes (weakened immunity, disease, or trauma), the so-called non-pathogenic bacteria can become pathogenic and cause infections. Wounds are susceptible to contamination by microorganisms both externally and from internal sources in the body, such as the nasopharynx, skin, and gastrointestinal tract. Infection is the result of a constantly changing interaction between microorganisms, the human being as their host, and the environment around them. Exposure of the Gram-positive and Gram-negative bacteria strains to the host’s defense capacity interferes with wound healing and potentially dangerous changes in the body due to infection.
According to research, more than 23,000 people die each year in Europe from invasive (or systemic) infections caused by
The increasing level of pollution by microorganisms and infections creates the need for new antimicrobial agents. Therefore, the research on the development and application of polymer composites with antimicrobial activity is of great interest.
Plant-mediated synthesis imparts several advantages to metal nanoparticles (MNPs) technology for the development of alternative products against infectious diseases. Indeed, most of green MNPs from plant-derived materials are highly effective and nonspecific antimicrobial agents, showing remarkable activities against the growth of a broad spectrum of bacterial and fungal species, in both planktonic and biofilm forms, including nosocomial and multidrug-resistant strains [2, 3].
Materials with antimicrobial activity are abundant. One of the largest groups is natural or synthetic antibiotics, which inhibits the appropriate stage of synthesis of the microorganism’s cellular proteins. However, excessive use of antibiotics has led to the emergence of strains of bacteria that are resistant to most antibiotics, posing a significant risk to public health. As a result, other substances with antimicrobial activity are increasingly being used. Their nature can be very different. These are, in particular, substances of natural origin: vegetable (various essential oils, medicinal plant extracts, etc.) and animal (e.g., lysozyme, lactoferrin), microbes (nisin, natamycin, etc.), as well as inorganic and organic synthetic and hybrid derivatives of a nature. Polymeric materials that are resistant to the colonization of microorganisms and the spread and multiplication of pathogenic microorganisms are also one of the groups of antimicrobials. They usually consist of a polymeric matrix and an embedded antimicrobial component.
The choice of antimicrobial modification methods depends on many factors (Figure 1).
The choice of antimicrobial modification method.
The main methods of antimicrobial modification of polymers are as presented in Figure 2.
The main methods of antimicrobial modification of polymers [
One of the most abundant groups of substances with antimicrobial activity suitable for polymer modification is inorganic compounds and metal nanoparticles. This group consists of metals (Ag, Au, Cu, etc.), metal oxides (ZnO, TiO2, etc.) [5], nonmetallic oxides (SiO2). In most cases, the size of antimicrobial nanomaterials ranges from 1 to 100 nm. They can be of organic or inorganic origin, but inorganic substances are most commonly used. Nanoparticles are the most widely used because they have broad-spectrum antibacterial properties against both Gram-negative and Gram-positive bacterial strains [6]. The main reason why nanoparticles are an alternative to antibiotics is their ability to inhibit multiresistant microorganisms in some cases. Nanoparticles have a large surface area that increases interaction with microorganisms, resulting in strong antimicrobial activity. Nanoparticles with a smaller size and a higher surface area to weight ratio are more efficient at breaking biofilms. The particle shape also has a significant effect on the degradation efficiency of biofilms (e.g., rod-shaped particles are much more efficient than spherical forms). There are various methods for the synthesis of nanoparticles, which can be divided into two main classes: (1) bottom-up, and (2) from top to bottom [7].
In general, the chemical, physical, mechanical, and antimicrobial properties of nanoparticles depend on their chosen precursor. Nanoparticle microorganisms act in different ways, and the mechanism of their action depends on the origin of the nanoparticle [8]. Nanoparticles have antibacterial (inhibits DNA replication, enzyme functions, etc.), antiviral (blocks the attachment of viruses to the cell wall), antifungal (breaks down the cell membrane), and other effects.
Nanotechnology is the science, engineering, and technology that studies matter at the atomic, molecular, or supramolecular levels to yield nanometric materials and nanosystems with improved properties such as high surface-to-volume ratio and high dispersion in solution. With size typically ranging between 1 and 100 nm, these nanomaterials and nanosystems can be synthesized by chemical, physical. and/or biological methods [9]. In comparison with chemical and physical methods that involve costly and toxic chemicals, the biological synthesis pathway based on the usage of biological sources (plants, bacteria, fungi, and algae) is hoisted as a real rescue route. In spite of that, the biological methods do not envisage the use of toxic catalysts and reagents, dealing exceptionally with the intracellularly or extracellularly produced metabolites within fermentation routes, and this method requires big input of costly materials, well-developed protocols and guidelines, and microbiological hands-on experience to ensure cell culture and nanoparticles purification under highly aseptic conditions.
In contrast, the use of plant-derived extracts, juice, hydrolysates, etc., for the biosynthesis of metal nanoparticles (MNPs) seems to be an environmentally friendly, cost-effective, robust, sustainable alternative with moderate reaction conditions [10]. The plant-mediated synthesis of nanoparticles is also biocompatible, clinically adaptable, and easily up-scalable for industrial production [11]. Plants could represent continuous source of natural antioxidants and antimicrobials (polyphenols, flavonoids, tannins, terpenoids, alkaloids, essential oils, etc.) suitable for green synthesis of nanoparticles with desirable properties. Under proper extraction conditions dealing with nontoxic organic solvents, diverse spectrum of non-deleterious reducing agents could be acquired [12].
Recent evidence in the field of nanotechnology revealed that the morphological parameters of nanoparticles (e.g., size and shape) can be modulated by varying the concentrations of bioactive compounds and reaction conditions (e.g., temperature and pH). Due to multiple therapeutic and biological activities such as antioxidative, antimicrobial, anti-inflammatory, anticancer, eugenol as a representative of phenylpropanoids received tremendous interest among researchers. The crude extracts recovered from such herbal plants as Lamiaceae, Lauraceae, Myrtaceae, and Myristicaceae, the major compound of which was eugenol, have been investigated in terms of reducing ability for nanoparticles synthesis. However, less explored are other sources of this unique molecule, especially by-products that also could provide adequate quantities of eugenol. Considering the evidence on the presence of eugenol, a principal component of lignin in cereals and their by-products (bran), and already established protocol for lignocellulose biomass hydrolysis, it is speculated that the process of biorefining could represent a sustainable and reliable way of bran utilization for the production of eugenol-based nanoparticles, thereby contributing to waste reduction. Additionally, using different sources of metals (salts or oxide) in combination with plants, the biological reduction method allows the synthesis of a large number of green MNPs, including silver (Ag), gold (Au), zinc oxide (ZnO), platinum (Pt), palladium (Pd), copper (Cu), iron oxide (Fe2O3 and Fe3O4), nickel oxide (NiO), magnesium oxide (MgO), titanium dioxide (TiO2), and indium oxide (In2O3).
Considering the above, exploration of the plant systems as potential bio-factories for MNPs has gained considerable attention, especially for researchers working in the field of phytonanotechnology, pharmaceutical, and clinical microbiology as well as medicine [7]. Indeed, due to the surging popularity of green methods, more than 2000 research papers and reviews related to antibacterial, antifungal, and antibiofilm properties of MNPs have been published. Noteworthy, most of the reviews and research articles published so far focused mainly on predicting the antimicrobial mechanisms of MNPs and parameters that may influence their antibacterial, antifungal, and activities such as.
Unfortunately, it appears from these reviews that the methods used for assessing the antibacterial, antifungal, and antibiofilm efficiency of MNPs are only partially elaborated in terms of standardization process; therefore, it is hard to correlate or compare data from different studies to pinpoint the high values of antimicrobial nanoparticles. Moreover, such methodologies and models are usually hard to extrapolate to real products.
Metal nanoparticles can affect the bacterium even in several ways, causing extremely strong antimicrobial activity (Figure 3). The most common and widely used silver nanoparticles, elemental silver has been widely used as an antimicrobial agent since ancient times. To improve their antibacterial activity and reduce their toxicity, silver ions can be transformed into metallic silver nanoparticles through biological and biomimetic methods of synthesis. Green AgNPs have demonstrated the ability to reduce microbial infections in the skin and burn wounds and prevent bacterial colonization on the surface of various medical devices such as catheters and prostheses. Acting as capping agents, different multifunctional phytochemicals contribute efficiently to these antimicrobial activities [8]. Moreover, AgNPs can express synergism with standard antibiotics such as gentamycin and streptomycin [13]. Hence, these combinations can effectively be used against antibiotic-resistant pathogens. Additionally, antifungal activities of AgNPs have extensively been studied and demonstrated in the literature [14]. In the frame of the fight against antibiotic resistance, green synthesized AgNPs may be used as vehicles to transport oligonucleotide-based antimicrobial. Their synthesis can be performed by physical, chemical, or biological methods. Particle size, morphology, and antimicrobial activity differ according to the chosen method [15].
Mechanisms of antimicrobial action of nanoparticles on bacterial cells.
Numerous studies have shown that silver nanoparticles, in both colloidal and ionic forms, have a broader spectrum of antibacterial activity than most other nanoparticles. Due to their unique optical, electrical, and chemical properties, silver nanostructures are widely used in a variety of industries. However, they are most commonly used in health care and medicine due to their strong antimicrobial activity against many pathogenic microorganisms—Gram-positive, Gram-negative, and antibiotic-resistant bacterial species, fungi, and viruses.
Rapid wound healing is due to decreased matrix metalloproteinase and increased neutrophil apoptosis in the wound induced by silver compounds. Matrix metalloproteinase is thought to be able to initiate inflammation and thus slow wound healing, so its regulation is very important [16]. Silver nanoparticles are also used in bone cement in various disinfectants [13] as antimicrobial agents. Beside this, their effect depends on several factors presented in the figure (Figure 4).
Factors determining the antimicrobial activity of silver nanoparticles.
Antimicrobial activity of silver ions is obtained by reacting with the main components of the bacterium:
cell wall and plasma membrane,
DNA and proteins.
Due to the small size and very large specific surface area, silver nanoparticles adhere firmly to the surface of the bacterium. Silver ions, by interacting with the bacterial cell membrane and the sulfur compounds present in its proteins, impair its functionality. Further, silver nanoparticles penetrate the cell and damage DNA. Silver ions react with phosphorus compounds present in DNA, disrupting the process of DNA replication, which inhibits bacterial proliferation. They also degrade bacterial proteins, especially enzymes that catalyze metabolic reactions and other vital cellular processes. In addition, nanoparticles lead to the formation of reactive oxygen species, which are active and unstable molecules that can damage cellular DNA, protein structures, and cell membranes [17].
The antimicrobial activity of silver ions in Gram-positive and Gram-negative bacterial cultures may be different due to differences in bacterial cell structure. The cell wall of Gram-positive and Gram-negative bacteria has a complex, semi-rigid structure. The structure of the wall is very important because it determines the ability of the bacteria to cause disease and resistance to certain antibiotics. The wall thickness of the bacterial cells is unequal. The cell wall of Gram-positive prokaryotes is composed of a network of macromolecules called peptidoglycan or murein, polysaccharides, lipids, and proteins. The wall thickness is much higher (20–80 nm) than that of Gram-negative bacteria. Their prokaryotic cell wall is composed of several layers: The inner dense electron layer (2–3 nm) is composed of peptidoglycan, two dense electron bands separated by an electron-conducting cavity, a space separated by the periplasmic cavity of the cytoplasmic membrane. The cell wall of Gram-positive microorganisms adheres closely to the cytoplasmic membrane [18]. These differences between bacterial species lead to unequal interactions between antimicrobial compounds. It is clear that metal nanoparticles are promising as antimicrobial agents and therapeutic agents due to their biological, physical, and chemical properties. They can solve many problems in the field of nanomedicine. However, there is a lack of knowledge about the long-term effects of nanoparticles on human health and the environment. Nanoparticles are stable and can accumulate in the environment; they have a tendency to agglomerate and can therefore change their dimensions. Toxicity studies of nanoparticles have shown that metal nanoparticles can act at the organ, tissue, cell, muscle, and protein levels. Nanoparticles are extremely small in size and can easily spread through air or water and adversely affect the skin, lungs, and brain (especially nanoparticles with dimensions 10 nm).
Therefore, the search for other substances with antimicrobial activity, such as the use of plant-derived substances to obtain antimicrobial compounds, is intensifying [19].
The aim was to compare the morphological differences of tomato pulp by variety. From Figure 5, the micrographs presented by SEM can show that the tomato particles are irregular in shape, with an uneven, layered surface, and that the particles appear to be composed of discrete slender shapes without any visible particles on the surface. The average particle diameter is very uneven as it was not fractionated, but the particle size could be harmonized by choosing milling techniques and conditions. Also, the particle size may vary depending on the desired properties.
SEM images of tomato by-products particles.
The aim is to obtain stable and externally resistant colloidal solutions of silver nanoparticles and to investigate the antimicrobial efficacy of synthesized nanoparticles. Silver nanoparticles (AgNPs) were obtained by crude metal synthesis by reducing and stabilizing silver nitrate in extracts from bioactive compounds.
The morphology of lyophilized AgNPs of biologically active tomato pulp used in the work was investigated by SEM methods. From Figure 6, the microstructures of tomato by-products AgNPs can be concluded from the irregularly shaped particles but do not form agglomerations, which will have a positive effect on antimicrobial activity. From the photos provided by TEM, we can see a clearer morphology of the particles. The particles are spherical and do not form agglomerates (Figure 6).
Tomatoes “Vilina” by-products TEM micrographs, surface EDS spectra, and elemental analysis.
In this case, individual agglomerations can already be observed. Scanning of metal particles at selected locations where AgNPs are suspected shows peaks in the 3.0 keV region of the EDS spectra that can be attributed to silver binding energy, and this can be detected at first and third samples. In second, sample AgNPs could not be found, but the biomatrices had antimicrobial activity. Therefore, we can say that the particles formed. With the help of TEM microscopy, we can see that the particles obtained are particles with a clear spherical shape, but in individual cases we can observe the formation of agglomerates (Figure 7).
Tomatoes “Laukiai” by-products TEM micrographs, surface EDS spectra, and elemental analysis.
The TEM images show an uneven surface with AgNPs. A high silver content in the biomatrix was identified (Figure 8). The particles remain irregular in shape and do not tend to form large aggregates, which is likely to have a positive effect on antimicrobial activity. From the presented photos, we can see the particle shape, size distribution, and agglomeration tendency of AgNPs. In this case, the largest particles are obtained. Also in their form the resulting spheres. The particles obtained have a relatively high polydispersity, which is likely to have a positive effect on antimicrobial activity.
Tomatoes by-products mix TEM micrographs, surface EDS spectra, and elemental analysis.
The antibacterial activity of organic colloidal solutions of AgNPs was tested for both Gram-negative and Gram-positive bacterial strains and fungi. From the results presented in Table 1, it can be concluded that silver nanoparticles in organic media actively interact with the bacterial membrane and disrupt their functions.
Reference (standard) cultures of microorganisms | Samples | |||||
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | |
0.0 ± 0.0 | 10.8 ± 0.1 | 0.0 ± 0.0 | 10.10 ± 0.1 | 0.0 ± 0.0 | 12.2 ± 0.1 | |
0.0 ± 0.0 | 9.7 ± 0.3 | 0.0 ± 0.0 | 10.1 ± 0.3 | 0.0 ± 0.0 | 11.5 ± 0.2 | |
0.0 ± 0.0 | 10.1 ± 0.1 | 0.0 ± 0.0 | 11.5 ± 0.1 | 0.0 ± 0.0 | 13.5 ± 0.1 | |
0.0 ± 0.0 | 5.8 ± 0.3 | 0.0 ± 0.0 | 5.6 ± 0.2 | 0.0 ± 0.0 | 6.2 ± 0.2 | |
0.0 ± 0.0 | 5.3 ± 0.1 | 0.0 ± 0.0 | 4.9 ± 0.2 | 0.0 ± 0.0 | 5.5 ± 0.4 | |
0.0 ± 0.0 | 4.2 ± 0.1 | 0.0 ± 0.0 | 3.5 ± 0.3 | 0.0 ± 0.0 | 4.1 ± 0.2 | |
0.0 ± 0.0 | 6.8 ± 0.2 | 0.0 ± 0.0 | 6.5 ± 0.1 | 0.0 ± 0.0 | 7.1 ± 0.4 | |
0.0 ± 0.0 | 8.7 ± 0.2 | 0.0 ± 0.0 | 9.7 ± 0.1 | 0.0 ± 0.0 | 6.9 ± 0.2 | |
0.0 ± 0.0 | 7.6 ± 0.3 | 0.0 ± 0.0 | 8.2 ± 0.5 | 0.0 ± 0.0 | 5.4 ± 0.1 | |
0.0 ± 0.0 | 5.3 ± 0.2 | 0.0 ± 0.0 | 7.4 ± 0.4 | 0.0 ± 0.0 | 6.4 ± 0.3 | |
0.0 ± 0.0 | 4.4 ± 0.1 | 0.0 ± 0.0 | 6.3 ± 0.2 | 0.0 ± 0.0 | 5.7 ± 0.2 |
Antimicrobial activity of the greenly synthesized AgNPs.
The results of the antifungal efficacy studies of AgNPs are presented inTable 1. Two different fungal cultures were selected:
It is clear that metal nanoparticles are promising as antimicrobial agents and therapeutic agents due to their biological, physical, and chemical properties. They can solve many problems in the field of nanomedicine. However, there is a lack of knowledge about the long-term effects of nanoparticles on human health and the environment. Nanoparticles are stable and can accumulate in the environment, and they have a tendency to agglomerate and can therefore change their dimensions. Toxicity studies of nanoparticles have shown that metal nanoparticles can act on the organ, tissue, cell, muscle, and protein levels. Nanoparticles are extremely small in size and can easily spread through air or water and adversely affect the skin, lungs, and brain (especially nanoparticles with dimensions ≤10 nm). Therefore, the search for other substances with antimicrobial activity, such as the use of plant-derived substances to obtain antimicrobial compounds, is intensifying.
Green nanoparticles obtained by green synthesis methods, which have a wide range of antibacterial properties against both Gram-negative and Gram-positive bacterial strains and fungi, expand their applications in orthopedics, biomedicine, and medicine, as well as in other industries. Recently, the range of substances resistant to microbial colonization and multiplication of pathogenic microorganisms are increasing due to the increasing use of extracts of medicinal plants and plant by-products, which are strong antioxidants with anticancer, antibacterial, anti-inflammatory, antiallergic, antiviral, hepatoprotective effects. One of the most important antioxidants accumulated in plants is phenolic compounds, the mechanism of action of which is related to their ability to neutralize free radicals, protect against diseases caused by oxidative stresses, and reduce various forms of reactive oxygen species. It can be assumed that the modification of green nanoparticles with multifunctional hybrid particles can increase and expand their scope. Such antimicrobial and functional biomatrices are obtained using secondary by-products and Ag.
Stable colloidal solutions of AgNPs with high antibacterial activity in organic media have been obtained, which completely inhibit various bacterial cultures.
This study was financed by the Lithuanian Research Centre for Agriculture and Forestry and attributed to the long-term research program “Horticulture: agrobiological foundations and technologies.”
The authors declare no conflict of interest.
AgNPs Scanning electron microscope Transmission electron microscopy Metal nanoparticles
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Finally, the studies that show the effect of the implementation of these methods in different fields of medical training are summarized and presented.",book:{id:"6211",slug:"medical-and-surgical-education-past-present-and-future",title:"Medical and Surgical Education",fullTitle:"Medical and Surgical Education - Past, Present and Future"},signatures:"Panteleimon Pantelidis, Angeliki Chorti, Ioanna Papagiouvanni,\nGeorgios Paparoidamis, Christos Drosos, Thrasyvoulos\nPanagiotakopoulos, Georgios Lales and Michail Sideris",authors:[{id:"211650",title:"M.D.",name:"Panteleimon",middleName:null,surname:"Pantelidis",slug:"panteleimon-pantelidis",fullName:"Panteleimon Pantelidis"},{id:"211654",title:"Ms.",name:"Angeliki",middleName:null,surname:"Chorti",slug:"angeliki-chorti",fullName:"Angeliki Chorti"},{id:"220557",title:"Ms.",name:"Ioanna",middleName:null,surname:"Papagiouvanni",slug:"ioanna-papagiouvanni",fullName:"Ioanna Papagiouvanni"},{id:"220558",title:"Mr.",name:"Georgios",middleName:null,surname:"Paparoidamis",slug:"georgios-paparoidamis",fullName:"Georgios Paparoidamis"},{id:"220559",title:"Mr.",name:"Georgios",middleName:null,surname:"Lales",slug:"georgios-lales",fullName:"Georgios Lales"},{id:"220560",title:"Mr.",name:"Thrasyvoulos",middleName:null,surname:"Panagiotakopoulos",slug:"thrasyvoulos-panagiotakopoulos",fullName:"Thrasyvoulos Panagiotakopoulos"},{id:"220561",title:"Mr.",name:"Christos",middleName:null,surname:"Drosos",slug:"christos-drosos",fullName:"Christos Drosos"},{id:"220562",title:"Dr.",name:"Michail",middleName:null,surname:"Sideris",slug:"michail-sideris",fullName:"Michail Sideris"}]},{id:"50915",doi:"10.5772/63266",title:"Doped Bioactive Glass Materials in Bone Regeneration",slug:"doped-bioactive-glass-materials-in-bone-regeneration",totalDownloads:3504,totalCrossrefCites:13,totalDimensionsCites:34,abstract:"In the arena of orthopaedic surgery, autograft is considered to be the gold standard for correction of fracture repair or other bone pathologies. But, it has some limitations such as donor site morbidity and shortage of supply, which evolved the use of allograft that also has some disadvantages such as immunogenic response to the host, low osteogenicity as well as possibilities of disease transmission. Despite the benefits of autografts and allografts, the limitations of each have necessitated the pursuit of alternatives biomaterials that has the ability to initiate osteogenesis, and the graft should closely mimic the natural bone along with regeneration of fibroblasts. A variety of artificial materials such as demineralised bone matrix, coralline hydroxyapatite and calcium phosphate-based ceramics such as hydroxyapatite (HA), β-tricalcium phosphate (β-TCP) and bioactive glass have been used over the decades to fill bone defects almost without associated soft tissue development. Most of them were having only the properties of osteointegration and osteoconduction. Only bioactive glass possesses osteogenic property that stimulates proliferation and differentiation of osteoprogenitor cells and in some cases influencing the fibroblastic properties. But, this material has also some disadvantages such as short-term and low mechanical strength along with decreased fracture resistance; but, this was further minimised by ion doping that positively enhanced new bone formation. There are many metal ions such as magnesium (Mg), strontium (Sr), manganese (Mn), iron (Fe), zinc (Zn), silver (Ag) and some rare earths that have been doped successfully into bioactive glass to enhance their mechanical and biological properties. In some of the cases, mesoporous bioactive glass materials with or without such doping have also been employed (with homogeneous distribution of pores in the size ranging between 2 and 50 nm). These biomaterials can be served as scaffold for bone regeneration with adequate mechanical properties to restore bone defects and facilitate healing process by regeneration of soft tissues as well. This chapter encompasses the use of bioactive glass in bulk and mesoporous form with doped therapeutic ions, their role in bone tissue regeneration, use as delivery of growth factors as well as coating material for orthopaedic implants.",book:{id:"5164",slug:"advanced-techniques-in-bone-regeneration",title:"Advanced Techniques in Bone Regeneration",fullTitle:"Advanced Techniques in Bone Regeneration"},signatures:"Samit Kumar Nandi, Arnab Mahato, Biswanath Kundu and Prasenjit\nMukherjee",authors:[{id:"60514",title:"Dr.",name:"Samit",middleName:null,surname:"Nandi",slug:"samit-nandi",fullName:"Samit Nandi"}]},{id:"37120",doi:"10.5772/29607",title:"Trigeminocardiac Reflex in Neurosurgery - Current Knowledge and Prospects",slug:"the-trigeminocardiac-reflex-in-neurosurgery-current-knowledge-and-prospects",totalDownloads:3438,totalCrossrefCites:10,totalDimensionsCites:27,abstract:null,book:{id:"749",slug:"explicative-cases-of-controversial-issues-in-neurosurgery",title:"Explicative Cases of Controversial Issues in Neurosurgery",fullTitle:"Explicative Cases of Controversial Issues in Neurosurgery"},signatures:"Amr Abdulazim, Martin N. Stienen, Pooyan Sadr-Eshkevari, Nora Prochnow, Nora Sandu, Benham Bohluli and Bernhard Schaller",authors:[{id:"78171",title:"Prof.",name:"Bernhard",middleName:null,surname:"Schaller",slug:"bernhard-schaller",fullName:"Bernhard Schaller"},{id:"78525",title:"Mr.",name:"Amr",middleName:null,surname:"Abdulazim",slug:"amr-abdulazim",fullName:"Amr Abdulazim"},{id:"78530",title:"Dr",name:"Pooyan",middleName:null,surname:"Sadr-Eshkevari",slug:"pooyan-sadr-eshkevari",fullName:"Pooyan Sadr-Eshkevari"},{id:"126039",title:"Dr.",name:"Martin",middleName:"Nikolaus",surname:"Stienen",slug:"martin-stienen",fullName:"Martin Stienen"},{id:"126040",title:"Dr.",name:"Nora",middleName:null,surname:"Prochnow",slug:"nora-prochnow",fullName:"Nora Prochnow"},{id:"126041",title:"Dr.",name:"Benham",middleName:null,surname:"Bohluli",slug:"benham-bohluli",fullName:"Benham Bohluli"}]},{id:"26863",doi:"10.5772/26362",title:"The Bearing Surfaces in Total Hip Arthroplasty – Options, Material Characteristics and Selection",slug:"the-bearing-surfaces-in-total-hip-arthroplasty-options-material-characteristics-and-selection",totalDownloads:9530,totalCrossrefCites:10,totalDimensionsCites:21,abstract:null,book:{id:"938",slug:"recent-advances-in-arthroplasty",title:"Recent Advances in Arthroplasty",fullTitle:"Recent Advances in Arthroplasty"},signatures:"Hamid Reza Seyyed Hosseinzadeh, Alireza Eajazi and Ali Sina Shahi",authors:[{id:"66361",title:"Dr.",name:"Alireza",middleName:null,surname:"Eajazi",slug:"alireza-eajazi",fullName:"Alireza Eajazi"},{id:"74857",title:"Dr.",name:"Hamid Reza",middleName:null,surname:"Seyyed Hosseinzadeh",slug:"hamid-reza-seyyed-hosseinzadeh",fullName:"Hamid Reza Seyyed Hosseinzadeh"},{id:"173207",title:"Dr.",name:"Alisina",middleName:null,surname:"Shahi",slug:"alisina-shahi",fullName:"Alisina Shahi"}]}],mostDownloadedChaptersLast30Days:[{id:"65467",title:"Anesthesia Management for Large-Volume Liposuction",slug:"anesthesia-management-for-large-volume-liposuction",totalDownloads:6203,totalCrossrefCites:1,totalDimensionsCites:2,abstract:"The apparent easiness with which liposuction is performed favors that patients, young surgeons, and anesthesiologists without experience in this field ignore the many events that occur during this procedure. Liposuction is a procedure to improve the body contour and not a surgery to reduce weight, although recently people who have failed in their plans to lose weight look at liposuction as a means to contour their body figure. Tumescent liposuction of large volumes requires a meticulous selection of each patient; their preoperative evaluation and perioperative management are essential to obtain the expected results. The various techniques of general anesthesia are the most recommended and should be monitored in the usual way, as well as monitoring the total doses of infiltrated local anesthetics to avoid systemic toxicity. The management of intravenous fluids is controversial, but the current trend is the restricted use of hydrosaline solutions. The most feared complications are deep vein thrombosis, pulmonary thromboembolism, fat embolism, lung edema, hypothermia, infections and even death. The adherence to the management guidelines and prophylaxis of venous thrombosis/thromboembolism is mandatory.",book:{id:"6221",slug:"anesthesia-topics-for-plastic-and-reconstructive-surgery",title:"Anesthesia Topics for Plastic and Reconstructive Surgery",fullTitle:"Anesthesia Topics for Plastic and Reconstructive Surgery"},signatures:"Sergio Granados-Tinajero, Carlos Buenrostro-Vásquez, Cecilia\nCárdenas-Maytorena and Marcela Contreras-López",authors:[{id:"273532",title:"Dr.",name:"Sergio Octavio",middleName:null,surname:"Granados Tinajero",slug:"sergio-octavio-granados-tinajero",fullName:"Sergio Octavio Granados Tinajero"}]},{id:"42855",title:"Critical Care Issues After Major Hepatic Surgery",slug:"critical-care-issues-after-major-hepatic-surgery",totalDownloads:8935,totalCrossrefCites:2,totalDimensionsCites:2,abstract:null,book:{id:"3164",slug:"hepatic-surgery",title:"Hepatic Surgery",fullTitle:"Hepatic Surgery"},signatures:"Ashok Thorat and Wei-Chen Lee",authors:[{id:"52360",title:"Prof.",name:"Wei-Chen",middleName:null,surname:"Lee",slug:"wei-chen-lee",fullName:"Wei-Chen Lee"},{id:"157213",title:"Dr.",name:"Ashok",middleName:null,surname:"Thorat",slug:"ashok-thorat",fullName:"Ashok Thorat"}]},{id:"72175",title:"Fontan Operation: A Comprehensive Review",slug:"fontan-operation-a-comprehensive-review",totalDownloads:1299,totalCrossrefCites:3,totalDimensionsCites:2,abstract:"Since the first description of the Fontan operation in the early 1970s, a number of modifications have been introduced and currently staged, total cavopulmonary connection with fenestration has become the most commonly used multistage surgery in diverting the vena caval blood flow into the lungs. The existing ventricle, whether it is left or right, is utilized to supply systemic circuit. During Stage I, palliative surgery is performed, usually at presentation in the neonatal period/early infancy, on the basis of pathophysiology of the cardiac defect. During Stage II, a bidirectional Glenn procedure is undertaken in which the superior vena caval flow is diverted into the lungs at an approximate age of 6 months. During Stage IIIA, the blood flow from the inferior vena cava (IVC) is rerouted into the pulmonary arteries, typically by an extra-cardiac conduit along with a fenestration, generally around 2 years of age. During Stage IIIB, the fenestration is closed by transcatheter methodology 6–12 months after Stage IIIA. The evolution of Fontan concepts, the indications for Fontan surgery, and the results of old and current types of Fontan operation form the focus of this review.",book:{id:"9585",slug:"advances-in-complex-valvular-disease",title:"Advances in Complex Valvular Disease",fullTitle:"Advances in Complex Valvular Disease"},signatures:"P. Syamasundar Rao",authors:[{id:"68531",title:"Dr.",name:"P. Syamasundar",middleName:null,surname:"Rao",slug:"p.-syamasundar-rao",fullName:"P. Syamasundar Rao"}]},{id:"45712",title:"Serdev Sutures® in Middle Face",slug:"serdev-sutures-in-middle-face",totalDownloads:4952,totalCrossrefCites:0,totalDimensionsCites:0,abstract:null,book:{id:"2989",slug:"miniinvasive-face-and-body-lifts-closed-suture-lifts-or-barbed-thread-lifts",title:"Miniinvasive Face and Body Lifts",fullTitle:"Miniinvasive Face and Body Lifts - Closed Suture Lifts or Barbed Thread Lifts"},signatures:"Nikolay Serdev",authors:[{id:"32585",title:"Dr.",name:"Nikolay",middleName:null,surname:"Serdev",slug:"nikolay-serdev",fullName:"Nikolay Serdev"}]},{id:"55812",title:"Postural Restoration: A Tri-Planar Asymmetrical Framework for Understanding, Assessing, and Treating Scoliosis and Other Spinal Dysfunctions",slug:"postural-restoration-a-tri-planar-asymmetrical-framework-for-understanding-assessing-and-treating-sc",totalDownloads:7701,totalCrossrefCites:0,totalDimensionsCites:1,abstract:"Current medical practice does not recognize the influence of innate, physiological, human asymmetry on scoliosis and other postural disorders. Interventions meant to correct these conditions are commonly based on symmetrical models of appearance and do not take into account asymmetric organ weight distribution, asymmetries of respiratory mechanics, and dominant movement patterns that are reinforced in daily functional activities. A model of innate, human asymmetry derived from the theoretical framework of the Postural Restoration Institute® (PRI) explicitly describes the physiological, biomechanical, and respiratory components of human asymmetry. This model is important because it gives an accurate baseline for understanding predisposing factors for the development of postural disorders, which, without intervention, will likely progress to structural dysfunction. Clinical tests to evaluate tri-planar musculoskeletal relationships and function, developed by PRI, are based on this asymmetric model. These tests are valuable for assessing patient’s status in the context of human asymmetry and in guiding appropriate exercise prescription and progression. Balancing musculoskeletal asymmetry is the aim of PRI treatment. Restoration of relative balance decreases pain, restores improved alignment, and strengthens appropriate muscle function. It can also halt the progression of dysfunction and improve respiration, quality of life, and appearance. PRI’s extensive body of targeted exercise progressions are highly effective due to their basis in the tri-planar asymmetric human model.",book:{id:"5816",slug:"innovations-in-spinal-deformities-and-postural-disorders",title:"Innovations in Spinal Deformities and Postural Disorders",fullTitle:"Innovations in Spinal Deformities and Postural Disorders"},signatures:"Susan Henning, Lisa C. Mangino and Jean Massé",authors:[{id:"204825",title:"Dr.",name:"Susan",middleName:null,surname:"Henning",slug:"susan-henning",fullName:"Susan Henning"},{id:"206242",title:"Dr.",name:"Lisa C",middleName:null,surname:"Mangino",slug:"lisa-c-mangino",fullName:"Lisa C Mangino"},{id:"206245",title:"Dr.",name:"Jean",middleName:null,surname:"Massé",slug:"jean-masse",fullName:"Jean Massé"}]}],onlineFirstChaptersFilter:{topicId:"202",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82020",title:"Minimally Invasive Transforaminal Lumbar Interbody Fusion: A Novel Technique and Technology with Case Series",slug:"minimally-invasive-transforaminal-lumbar-interbody-fusion-a-novel-technique-and-technology-with-case",totalDownloads:6,totalDimensionsCites:0,doi:"10.5772/intechopen.105187",abstract:"Minimally invasive spine surgery (MIS) transforaminal lumbar interbody fusion (MI-TLIF) has been utilized to treat a variety of spinal disorders. Like other minimally invasive spine surgery techniques and technology, the MI-TLIF approach has the potential to limit the morbidity associated with larger exposures required for open surgery. The MI-TLIF approach has a number of advantages over many other minimally invasive spine surgery approaches including direct decompression of neural elements, collection of morselized autograph from the surgical site to achieve high fusion rates, restoration of spinal canal diameter, foraminal diameter, disk height, and reduction of spondylolisthesis. In this chapter, we discuss a novel technique for performing MI-TLIF developed by the senior author who is a leading minimally invasive spine surgeon. The technique and technology illustrated in this chapter were developed out of a recognition of a need to reduce the learning curve for performing MI-TLIF, as well as need for a cost-effective method that provides a high fusion rate, excellent clinical outcomes, and low complication rate. The indications, surgical planning, postoperative care, complications, and patient outcomes in a large series will be reviewed using this novel MI-TLIF technique.",book:{id:"10634",title:"Minimally Invasive Spine Surgery - Advances and Innovations",coverURL:"https://cdn.intechopen.com/books/images_new/10634.jpg"},signatures:"Mick Perez-Cruet, Ramiro Pérez de la Torre and Siddharth Ramanathan"},{id:"78335",title:"Safety and Efficiency of Cervical Disc Arthroplasty in Ambulatory Surgery Centers",slug:"safety-and-efficiency-of-cervical-disc-arthroplasty-in-ambulatory-surgery-centers",totalDownloads:5,totalDimensionsCites:0,doi:"10.5772/intechopen.99589",abstract:"Introduction Anterior cervical surgeries have been safely performed in ambulatory surgery centers since 1995 with the first cases being one level anterior cervical discectomies without fusion, then in 1996, one level anterior cervical discectomies with fusion (ACDF). When it is was certain that outpatient fusion was safe, the number of ACDF levels slowly and methodically were increased to the now standard outpatient maximum of four level ACDF. During this evolution, with the introduction of arthroplasty surgery, one level arthroplasties were considered appropriate for outpatient surgery and now two-level outpatient cervical arthroplasties are routine and some three level arthroplasties have been performed with no additional morbidity compared to one level procedures. The author first reported a series of 27 patients in 2010 who underwent cervical disc replacement at an ASC. (Wohns, R. Safety and cost-effectiveness of outpatient cervical disc arthroplasty. Surg. Neurol. Int. 1, 77, 2010). The average operative time was 40 minutes and the patients were observed over a period of three hours prior to discharge. None of the patients had major complications and there were no reports of worsening or persistent pain. The results of a Delphi study in 2018 compared the safety and efficiency of one-level and two-level arthroplasty procedures performed in an ASC and in a hospital setting. (Gornet et al. Safety and Efficiency of Cervical Disc Arthroplasty in Ambulatory Surgery Centers vs Hospital Settings. Int’l J of Spine Surgery. Vol. 12, No.5, 2018, pp. 557-564). The study analyzed outcomes of 145 ASC patients, 348 hospital outpatients and 65 hospital inpatients and the conclusion was that both one and two-level arthroplasties may be performed safely in an ASC. Surgeries in ASCs are of shorter duration and performed with less blood loss without increased AEs. At the present time, there does not appear to be any contra-indication to performing the vast majority of cervical arthroplasties in an ambulatory surgery center (ASC). Furthermore, the cost of an outpatient arthroplasty is commonly 30% to 50% of the cost of hospital-based procedures.",book:{id:"10634",title:"Minimally Invasive Spine Surgery - Advances and Innovations",coverURL:"https://cdn.intechopen.com/books/images_new/10634.jpg"},signatures:"Richard N.W. Wohns"},{id:"82255",title:"Minimally Invasive Laminectomy for Lumbar Stenosis with Case Series of Patients with Multi-level (3 or More Levels) Stenosis",slug:"minimally-invasive-laminectomy-for-lumbar-stenosis-with-case-series-of-patients-with-multi-level-3-o",totalDownloads:28,totalDimensionsCites:0,doi:"10.5772/intechopen.105186",abstract:"Lumbar stenosis is the most common pathology seen and treated by spine surgeons. It is often seen in the elderly population who frequently have multiple medical co-morbidities. Traditional approaches remove the spinous process and detach paraspinous muscles to achieve adequate canal decompression. This approach can damage the posterior tension band leading to permanent muscle damage, scar tissue formation, iatrogenic flatback syndrome, and increase risk of adjacent segment disease requiring reoperation. Performing lumbar laminectomy in a cost-effective manner is critical in effectively treating patients with lumbar stenosis. This chapter reviews a minimally invasive muscle-sparing approach to treating lumbar stenosis. The technique is performed through a tubular retractor. Direct decompression of the spinal stenosis is achieved while preserving the paraspinous muscle attachments and spinous process. This technique has multiple advantages and can potentially reduce load stress on adjacent levels and subsequent adjacent level pathology leading to further surgical intervention. In addition, the procedure shows how facet fusion is performed using the patient’s own locally harvested drilled morselized autograph to achieve bilateral facet fusion. By fusing the facets, we have shown that restenosis at the operative level is less likely to occur. This chapter will review a case series of multilevel lumbar stenosis including clinical outcomes.",book:{id:"10634",title:"Minimally Invasive Spine Surgery - Advances and Innovations",coverURL:"https://cdn.intechopen.com/books/images_new/10634.jpg"},signatures:"Mick Perez-Cruet, Ramiro Pérez de la Torre and Siddharth Ramanathan"},{id:"80705",title:"Cervical Arthroplasty",slug:"cervical-arthroplasty",totalDownloads:38,totalDimensionsCites:0,doi:"10.5772/intechopen.102964",abstract:"Technological advances have allowed spine surgery to follow the trend toward minimally invasive surgery in general. Specifically, we have seen a corresponding rise in the popularity of cervical arthroplasty. For the treatment of cervical disc disease, arthroplasty is a less invasive option than the gold standard of cervical discectomy and arthrodesis, which by nature is more disruptive to surrounding tissues. Arthroplasty preserves the facets, maintains motion, and reduces the rate of adjacent segment breakdown. These factors counteract the negative impacts of fusion while maintaining the benefits. Arthroplasty implants themselves have become more streamlined to implant as well with less native bone destruction, and biomechanics more compatible with the native disc. While initial implants were ball and socket devices with complex fixation and plane-specific movements, later devices incorporated such motions as translation and compression. Viscoelastic components and materials more closely resembling native tissues afford a more biocompatible implant profile. Until cell-based therapies can successfully reproduce native tissue, we will rely on artificial components that closely resemble and assimilate them.",book:{id:"10634",title:"Minimally Invasive Spine Surgery - Advances and Innovations",coverURL:"https://cdn.intechopen.com/books/images_new/10634.jpg"},signatures:"Jason M. Highsmith"},{id:"80605",title:"Minimally Invasive Treatment of Spinal Metastasis",slug:"minimally-invasive-treatment-of-spinal-metastasis",totalDownloads:42,totalDimensionsCites:0,doi:"10.5772/intechopen.102485",abstract:"Advancements in the treatment of systemic cancer have improved life expectancy in cancer patients and consequently the incidence of spinal metastasis. Traditionally, open spinal approaches combined with cEBRT (conventional external beam radiation therapy) allowed for local tumor control as well as stabilization and decompression of the spine and neural elements, but these larger operations can be fraught with one complications and delayed healing as well as additional morbidity. Recently, minimally invasive spine techniques are becoming increasingly popular in the treatment of spinal metastasis for many reasons, including smaller incisions with less perioperative complications and potential for expedited time to radiation therapy. These techniques include kyphoplasty with radiofrequency ablation, percutaneous stabilization, laminectomy, and epidural tumor resection through tubular retractors, as well as minimally invasive corpectomy. These techniques combined with highly conformal stereotactic radiosurgery have led to the advent of separation surgery, which allows for decompression of neural elements while creating space between neural elements and the tumor so adequate radiation may be delivered, improving local tumor control. The versatility of these minimally invasive techniques has significantly improved the modern management of metastatic disease of the spine by protecting and restoring the patient’s quality of life while allowing them to quickly resume radiation and systemic treatment.",book:{id:"10634",title:"Minimally Invasive Spine Surgery - Advances and Innovations",coverURL:"https://cdn.intechopen.com/books/images_new/10634.jpg"},signatures:"Eric R. Mong and Daniel K. Fahim"},{id:"76620",title:"Minimally Invasive Lateral Approach for Anterior Spinal Cord Decompression in Thoracic Myelopathy",slug:"minimally-invasive-lateral-approach-for-anterior-spinal-cord-decompression-in-thoracic-myelopathy",totalDownloads:146,totalDimensionsCites:0,doi:"10.5772/intechopen.97669",abstract:"Myelopathy can result from a thoracic disc herniation (TDH) compressing the anterior spinal cord. Disc calcification and difficulty in accessing the anterior spinal cord pose an operative challenge. A mini-open lateral approach to directly decompress the anterior spinal cord can be performed with or without concomitant interbody fusion depending on pre-existing or iatrogenic spinal instability. Experience using stand-alone expandable spacers to achieve interbody fusion in this setting is limited. Technical advantages, risks and limitations of this technique are discussed. We conducted a retrospective chart review of all patients with thoracic and upper lumbar myelopathy treated with a lateral mini-open lateral approach. Review of the literature identified 6 other case series using similar lateral minimally invasive approaches to treat thoracic or upper lumbar disc herniation showing efficient and safe thoracic disc decompression procedure for myelopathy. This technique can be combined with interbody arthrodesis when instability is suspected.",book:{id:"10634",title:"Minimally Invasive Spine Surgery - Advances and Innovations",coverURL:"https://cdn.intechopen.com/books/images_new/10634.jpg"},signatures:"Edna E. Gouveia, Mansour Mathkour, Erin McCormack, Jonathan Riffle, Olawale A. Sulaiman and Daniel J. Denis"}],onlineFirstChaptersTotal:12},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:90,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:108,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:330,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:9,numberOfPublishedChapters:141,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:124,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:112,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:22,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:11,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"August 2nd, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:33,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"14",title:"Cell and Molecular Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",isOpenForSubmission:!0,editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",slug:"rosa-maria-martinez-espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",biography:"Dr. Rosa María Martínez-Espinosa has been a Spanish Full Professor since 2020 (Biochemistry and Molecular Biology) and is currently Vice-President of International Relations and Cooperation development and leader of the research group 'Applied Biochemistry” (University of Alicante, Spain). Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. Dr. Beydemir is also Rector of Bilecik Şeyh Edebali University, Turkey.",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",slug:"deniz-ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",biography:"Dr. Deniz Ekinci obtained a BSc in Chemistry in 2004, MSc in Biochemistry in 2006, and PhD in Biochemistry in 2009 from Atatürk University, Turkey. He studied at Stetson University, USA, in 2007-2008 and at the Max Planck Institute of Molecular Cell Biology and Genetics, Germany, in 2009-2010. Dr. Ekinci currently works as a Full Professor of Biochemistry in the Faculty of Agriculture and is the Head of the Enzyme and Microbial Biotechnology Division, Ondokuz Mayıs University, Turkey. He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. Dr. Ekinci serves as the Editor in Chief of four international books and is involved in the Editorial Board of several international journals.",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null},{id:"17",title:"Metabolism",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",isOpenForSubmission:!0,editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",slug:"yannis-karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",biography:"Yannis Karamanos, born in Greece in 1953, completed his pre-graduate studies at the Université Pierre et Marie Curie, Paris, then his Masters and Doctoral degree at the Université de Lille (1983). He was associate professor at the University of Limoges (1987) before becoming full professor of biochemistry at the Université d’Artois (1996). He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. His teaching areas are energy metabolism and regulation, integration and organ specialization and metabolic adaptation.",institutionString:null,institution:{name:"Artois University",institutionURL:null,country:{name:"France"}}},editorTwo:null,editorThree:null},{id:"18",title:"Proteomics",coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",isOpenForSubmission:!0,editor:{id:"200689",title:"Prof.",name:"Paolo",middleName:null,surname:"Iadarola",slug:"paolo-iadarola",fullName:"Paolo Iadarola",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSCl8QAG/Profile_Picture_1623568118342",biography:"Paolo Iadarola graduated with a degree in Chemistry from the University of Pavia (Italy) in July 1972. He then worked as an Assistant Professor at the Faculty of Science of the same University until 1984. In 1985, Prof. Iadarola became Associate Professor at the Department of Biology and Biotechnologies of the University of Pavia and retired in October 2017. Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. He is a Consultant Reviewer for several journals, including the Journal of Chromatography A, Journal of Chromatography B, Plos ONE, Proteomes, International Journal of Molecular Science, Biotech, Electrophoresis, and others. He is also Associate Editor of Biotech.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorTwo:{id:"201414",title:"Dr.",name:"Simona",middleName:null,surname:"Viglio",slug:"simona-viglio",fullName:"Simona Viglio",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRKDHQA4/Profile_Picture_1630402531487",biography:"Simona Viglio is an Associate Professor of Biochemistry at the Department of Molecular Medicine at the University of Pavia. She has been working since 1995 on the determination of proteolytic enzymes involved in the degradation process of connective tissue matrix and on the identification of biological markers of lung diseases. She gained considerable experience in developing and validating new methodologies whose applications allowed her to determine both the amount of biomarkers (Desmosine and Isodesmosine) in the urine of patients affected by COPD, and the activity of proteolytic enzymes (HNE, Cathepsin G, Pseudomonas aeruginosa elastase) in the sputa of these patients. Simona Viglio was also involved in research dealing with the supplementation of amino acids in patients with brain injury and chronic heart failure. She is presently engaged in the development of 2-DE and LC-MS techniques for the study of proteomics in biological fluids. The aim of this research is the identification of potential biomarkers of lung diseases. She is an author of about 90 publications (According to Scopus: H-Index: 23; According to WOS: H-Index: 20) on peer-reviewed journals, a member of the “Società Italiana di Biochimica e Biologia Molecolare,“ and a Consultant Reviewer for International Journal of Molecular Science, Journal of Chromatography A, COPD, Plos ONE and Nutritional Neuroscience.",institutionString:null,institution:{name:"University of Pavia",institutionURL:null,country:{name:"Italy"}}},editorThree:null}]},overviewPageOFChapters:{paginationCount:42,paginationItems:[{id:"82914",title:"Glance on the Critical Role of IL-23 Receptor Gene Variations in Inflammation-Induced Carcinogenesis",doi:"10.5772/intechopen.105049",signatures:"Mohammed El-Gedamy",slug:"glance-on-the-critical-role-of-il-23-receptor-gene-variations-in-inflammation-induced-carcinogenesis",totalDownloads:15,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Chemokines Updates",coverURL:"https://cdn.intechopen.com/books/images_new/11672.jpg",subseries:{id:"18",title:"Proteomics"}}},{id:"82875",title:"Lipidomics as a Tool in the Diagnosis and Clinical Therapy",doi:"10.5772/intechopen.105857",signatures:"María Elizbeth Alvarez Sánchez, Erick Nolasco Ontiveros, Rodrigo Arreola, Adriana Montserrat Espinosa González, Ana María García Bores, Roberto Eduardo López Urrutia, Ignacio Peñalosa Castro, María del Socorro Sánchez Correa and Edgar Antonio Estrella Parra",slug:"lipidomics-as-a-tool-in-the-diagnosis-and-clinical-therapy",totalDownloads:9,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Fatty Acids - Recent Advances",coverURL:"https://cdn.intechopen.com/books/images_new/11669.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"82440",title:"Lipid Metabolism and Associated Molecular Signaling Events in Autoimmune Disease",doi:"10.5772/intechopen.105746",signatures:"Mohan Vanditha, Sonu Das and Mathew John",slug:"lipid-metabolism-and-associated-molecular-signaling-events-in-autoimmune-disease",totalDownloads:17,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Fatty Acids - Recent Advances",coverURL:"https://cdn.intechopen.com/books/images_new/11669.jpg",subseries:{id:"17",title:"Metabolism"}}},{id:"82483",title:"Oxidative Stress in Cardiovascular Diseases",doi:"10.5772/intechopen.105891",signatures:"Laura Mourino-Alvarez, Tamara Sastre-Oliva, Nerea Corbacho-Alonso and Maria G. 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She is also the Global Harmonization Initiative (GHI)",institutionString:"Australian College of Business & Technology",institution:{name:"Kobe College",institutionURL:null,country:{name:"Japan"}}}]},{type:"book",id:"6820",title:"Keratin",subtitle:null,coverURL:"https://cdn.intechopen.com/books/images_new/6820.jpg",slug:"keratin",publishedDate:"December 19th 2018",editedByType:"Edited by",bookSignature:"Miroslav Blumenberg",hash:"6def75cd4b6b5324a02b6dc0359896d0",volumeInSeries:2,fullTitle:"Keratin",editors:[{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. 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She has more than fifteen years of teaching and research experience. She has published more than 550 scientific publications/communications, including 15 books, 50 book chapters, 100 original research papers, 380 research communications in national and international conferences, and 12 patents. She is a member of the editorial board of five journals and acts as a reviewer for several national and international journals. Her research interests include microalgal biotechnology with an emphasis on microalgae-based products.",institutionString:"Universidade Federal de Santa Maria",institution:{name:"Universidade Federal de Santa Maria",institutionURL:null,country:{name:"Brazil"}}}]},{type:"book",id:"7953",title:"Bioluminescence",subtitle:"Analytical Applications and Basic Biology",coverURL:"https://cdn.intechopen.com/books/images_new/7953.jpg",slug:"bioluminescence-analytical-applications-and-basic-biology",publishedDate:"September 25th 2019",editedByType:"Edited by",bookSignature:"Hirobumi Suzuki",hash:"3a8efa00b71abea11bf01973dc589979",volumeInSeries:4,fullTitle:"Bioluminescence - Analytical Applications and Basic Biology",editors:[{id:"185746",title:"Dr.",name:"Hirobumi",middleName:null,surname:"Suzuki",slug:"hirobumi-suzuki",fullName:"Hirobumi Suzuki",profilePictureURL:"https://mts.intechopen.com/storage/users/185746/images/system/185746.png",biography:"Dr. Hirobumi Suzuki received his Ph.D. in 1997 from Tokyo Metropolitan University, Japan, where he studied firefly phylogeny and the evolution of mating systems. He is especially interested in the genetic differentiation pattern and speciation process that correlate to the flashing pattern and mating behavior of some fireflies in Japan. He then worked for Olympus Corporation, a Japanese manufacturer of optics and imaging products, where he was involved in the development of luminescence technology and produced a bioluminescence microscope that is currently being used for gene expression analysis in chronobiology, neurobiology, and developmental biology. Dr. Suzuki currently serves as a visiting researcher at Kogakuin University, Japan, and also a vice president of the Japan Firefly Society.",institutionString:"Kogakuin University",institution:null}]}]},openForSubmissionBooks:{paginationCount:2,paginationItems:[{id:"12141",title:"Leadership - Advancing Great Leadership Practices and Good Leaders",coverURL:"https://cdn.intechopen.com/books/images_new/12141.jpg",hash:"85f77453916f1d80d80d88ee4fd2f2d1",secondStepPassed:!0,currentStepOfPublishingProcess:3,submissionDeadline:"July 1st 2022",isOpenForSubmission:!0,editors:[{id:"420133",title:"Dr.",name:"Joseph",surname:"Crawford",slug:"joseph-crawford",fullName:"Joseph Crawford"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null},{id:"12139",title:"Global Market and Trade",coverURL:"https://cdn.intechopen.com/books/images_new/12139.jpg",hash:"fa34af07c3a9657fa670404202f8cba5",secondStepPassed:!0,currentStepOfPublishingProcess:3,submissionDeadline:"July 21st 2022",isOpenForSubmission:!0,editors:[{id:"243649",title:"Dr.Ing.",name:"Ireneusz",surname:"Miciuła",slug:"ireneusz-miciula",fullName:"Ireneusz Miciuła"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},onlineFirstChapters:{paginationCount:9,paginationItems:[{id:"82936",title:"Soil Degradation Processes Linked to Long-Term Forest-Type Damage",doi:"10.5772/intechopen.106390",signatures:"Pavel Samec, Aleš Kučera and Gabriela Tomášová",slug:"soil-degradation-processes-linked-to-long-term-forest-type-damage",totalDownloads:3,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Forest Degradation Under Global Change",coverURL:"https://cdn.intechopen.com/books/images_new/11457.jpg",subseries:{id:"94",title:"Climate Change and Environmental Sustainability"}}},{id:"82777",title:"Sustainability and Social Investment: Community Microhydropower Systems in the Dominican Republic",doi:"10.5772/intechopen.105995",signatures:"Michela Izzo, Alberto Sánchez and Rafael Fonseca",slug:"sustainability-and-social-investment-community-microhydropower-systems-in-the-dominican-republic",totalDownloads:4,totalCrossrefCites:0,totalDimensionsCites:0,authors:null,book:{title:"Globalization and Sustainability - Recent Advances, New Perspectives and Emerging Issues",coverURL:"https://cdn.intechopen.com/books/images_new/11476.jpg",subseries:{id:"91",title:"Sustainable Economy and Fair Society"}}},{id:"82387",title:"Kept Promises? 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