Antigens tested as vaccine candidates against infestations by D. gallinae.\n
\r\n\tAnimal food additives are products used in animal nutrition for purposes of improving the quality of feed or to improve the animal’s performance and health. Other additives can be used to enhance digestibility or even flavour of feed materials. In addition, feed additives are known which improve the quality of compound feed production; consequently e.g. they improve the quality of the granulated mixed diet.
\r\n\r\n\tGenerally feed additives could be divided into five groups:
\r\n\t1.Technological additives which influence the technological aspects of the diet to improve its handling or hygiene characteristics.
\r\n\t2. Sensory additives which improve the palatability of a diet by stimulating appetite, usually through the effect these products have on the flavour or colour.
\r\n\t3. Nutritional additives, such additives are specific nutrient(s) required by the animal for optimal production.
\r\n\t4.Zootechnical additives which improve the nutrient status of the animal, not by providing specific nutrients, but by enabling more efficient use of the nutrients present in the diet, in other words, it increases the efficiency of production.
\r\n\t5. In poultry nutrition: Coccidiostats and Histomonostats which widely used to control intestinal health of poultry through direct effects on the parasitic organism concerned.
\r\n\tThe aim of the book is to present the impact of the most important feed additives on the animal production, to demonstrate their mode of action, to show their effect on intermediate metabolism and heath status of livestock and to suggest how to use the different feed additives in animal nutrition to produce high quality and safety animal origin foodstuffs for human consumer.
",isbn:"978-1-83969-404-2",printIsbn:"978-1-83969-403-5",pdfIsbn:"978-1-83969-405-9",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!1,hash:"8ffe43a82ac48b309abc3632bbf3efd0",bookSignature:"Prof. László Babinszky",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/10496.jpg",keywords:"Technological Feed Additives, Feed Industry, Quality of Compound Feed, Non-Antibiotic Growth Promoter, Product Quality, Additive Enzymes, Digestibility of Nutrients, NSP Enzymes, Farm Animals, Livestock, Immunity, Microbiome",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"November 24th 2020",dateEndSecondStepPublish:"December 22nd 2020",dateEndThirdStepPublish:"February 20th 2021",dateEndFourthStepPublish:"May 11th 2021",dateEndFifthStepPublish:"July 10th 2021",remainingDaysToSecondStep:"2 months",secondStepPassed:!0,currentStepOfPublishingProcess:4,editedByType:null,kuFlag:!1,biosketch:"Professor Emeritus from the University of Debrecen, Hungary who authored 297 publications (papers, book chapters) and edited 3 books. Member of various committees and chairman of the World Conference of Innovative Animal Nutrition and Feeding (WIANF).",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"53998",title:"Prof.",name:"László",middleName:null,surname:"Babinszky",slug:"laszlo-babinszky",fullName:"László Babinszky",profilePictureURL:"https://mts.intechopen.com/storage/users/53998/images/system/53998.jpg",biography:"László Babinszky is Professor Emeritus of animal nutrition at the University of Debrecen, Hungary. From 1984 to 1985 he worked at the Agricultural University in Wageningen and in the Institute for Livestock Feeding and Nutrition in Lelystad (the Netherlands). He also worked at the Agricultural University of Vienna in the Institute for Animal Breeding and Nutrition (Austria) and in the Oscar Kellner Research Institute in Rostock (Germany). From 1988 to 1992, he worked in the Department of Animal Nutrition (Agricultural University in Wageningen). In 1992 he obtained a PhD degree in animal nutrition from the University of Wageningen.He has authored 297 publications (papers, book chapters). He edited 3 books and 14 international conference proceedings. His total number of citation is 407. \r\nHe is member of various committees e.g.: American Society of Animal Science (ASAS, USA); the editorial board of the Acta Agriculturae Scandinavica, Section A- Animal Science (Norway); KRMIVA, Journal of Animal Nutrition (Croatia), Austin Food Sciences (NJ, USA), E-Cronicon Nutrition (UK), SciTz Nutrition and Food Science (DE, USA), Journal of Medical Chemistry and Toxicology (NJ, USA), Current Research in Food Technology and Nutritional Sciences (USA). From 2015 he has been appointed chairman of World Conference of Innovative Animal Nutrition and Feeding (WIANF).\r\nHis main research areas are related to pig and poultry nutrition: elimination of harmful effects of heat stress by nutrition tools, energy- amino acid metabolism in livestock, relationship between animal nutrition and quality of animal food products (meat).",institutionString:"University of Debrecen",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"University of Debrecen",institutionURL:null,country:{name:"Hungary"}}}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"25",title:"Veterinary Medicine and Science",slug:"veterinary-medicine-and-science"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"185543",firstName:"Maja",lastName:"Bozicevic",middleName:null,title:"Ms.",imageUrl:"https://mts.intechopen.com/storage/users/185543/images/4748_n.jpeg",email:"maja.b@intechopen.com",biography:"As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review, to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. Whether that be identifying an exceptional author and proposing an editorship collaboration, or contacting researchers who would like the opportunity to work with IntechOpen, I establish and help manage author and editor acquisition and contact."}},relatedBooks:[{type:"book",id:"7144",title:"Veterinary Anatomy and Physiology",subtitle:null,isOpenForSubmission:!1,hash:"75cdacb570e0e6d15a5f6e69640d87c9",slug:"veterinary-anatomy-and-physiology",bookSignature:"Catrin Sian Rutland and Valentina Kubale",coverURL:"https://cdn.intechopen.com/books/images_new/7144.jpg",editedByType:"Edited by",editors:[{id:"202192",title:"Dr.",name:"Catrin",surname:"Rutland",slug:"catrin-rutland",fullName:"Catrin Rutland"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1591",title:"Infrared Spectroscopy",subtitle:"Materials Science, Engineering and Technology",isOpenForSubmission:!1,hash:"99b4b7b71a8caeb693ed762b40b017f4",slug:"infrared-spectroscopy-materials-science-engineering-and-technology",bookSignature:"Theophile Theophanides",coverURL:"https://cdn.intechopen.com/books/images_new/1591.jpg",editedByType:"Edited by",editors:[{id:"37194",title:"Dr.",name:"Theophanides",surname:"Theophile",slug:"theophanides-theophile",fullName:"Theophanides Theophile"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3092",title:"Anopheles mosquitoes",subtitle:"New insights into malaria vectors",isOpenForSubmission:!1,hash:"c9e622485316d5e296288bf24d2b0d64",slug:"anopheles-mosquitoes-new-insights-into-malaria-vectors",bookSignature:"Sylvie Manguin",coverURL:"https://cdn.intechopen.com/books/images_new/3092.jpg",editedByType:"Edited by",editors:[{id:"50017",title:"Prof.",name:"Sylvie",surname:"Manguin",slug:"sylvie-manguin",fullName:"Sylvie Manguin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"3161",title:"Frontiers in Guided Wave Optics and Optoelectronics",subtitle:null,isOpenForSubmission:!1,hash:"deb44e9c99f82bbce1083abea743146c",slug:"frontiers-in-guided-wave-optics-and-optoelectronics",bookSignature:"Bishnu Pal",coverURL:"https://cdn.intechopen.com/books/images_new/3161.jpg",editedByType:"Edited by",editors:[{id:"4782",title:"Prof.",name:"Bishnu",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1373",title:"Ionic Liquids",subtitle:"Applications and Perspectives",isOpenForSubmission:!1,hash:"5e9ae5ae9167cde4b344e499a792c41c",slug:"ionic-liquids-applications-and-perspectives",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/1373.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"57",title:"Physics and Applications of Graphene",subtitle:"Experiments",isOpenForSubmission:!1,hash:"0e6622a71cf4f02f45bfdd5691e1189a",slug:"physics-and-applications-of-graphene-experiments",bookSignature:"Sergey Mikhailov",coverURL:"https://cdn.intechopen.com/books/images_new/57.jpg",editedByType:"Edited by",editors:[{id:"16042",title:"Dr.",name:"Sergey",surname:"Mikhailov",slug:"sergey-mikhailov",fullName:"Sergey Mikhailov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"371",title:"Abiotic Stress in Plants",subtitle:"Mechanisms and Adaptations",isOpenForSubmission:!1,hash:"588466f487e307619849d72389178a74",slug:"abiotic-stress-in-plants-mechanisms-and-adaptations",bookSignature:"Arun Shanker and B. Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"878",title:"Phytochemicals",subtitle:"A Global Perspective of Their Role in Nutrition and Health",isOpenForSubmission:!1,hash:"ec77671f63975ef2d16192897deb6835",slug:"phytochemicals-a-global-perspective-of-their-role-in-nutrition-and-health",bookSignature:"Venketeshwer Rao",coverURL:"https://cdn.intechopen.com/books/images_new/878.jpg",editedByType:"Edited by",editors:[{id:"82663",title:"Dr.",name:"Venketeshwer",surname:"Rao",slug:"venketeshwer-rao",fullName:"Venketeshwer Rao"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"4816",title:"Face Recognition",subtitle:null,isOpenForSubmission:!1,hash:"146063b5359146b7718ea86bad47c8eb",slug:"face_recognition",bookSignature:"Kresimir Delac and Mislav Grgic",coverURL:"https://cdn.intechopen.com/books/images_new/4816.jpg",editedByType:"Edited by",editors:[{id:"528",title:"Dr.",name:"Kresimir",surname:"Delac",slug:"kresimir-delac",fullName:"Kresimir Delac"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"70407",title:"Challenges for the Control of Poultry Red Mite (Dermanyssus gallinae)",doi:"10.5772/intechopen.90439",slug:"challenges-for-the-control-of-poultry-red-mite-em-dermanyssus-gallinae-em-",body:'\nThe poultry red mite (PRM), Dermanyssus gallinae (De Geer, 1778), is a hematophagous mite that affects mainly poultry [1] but also parasitizes other avian [2] and mammalian hosts [3, 4, 5], including humans [6]. PRM has a worldwide distribution and it constitutes a serious problem for the European egg-laying industry where the average prevalence is 80% with some countries reaching a prevalence higher than 90% of the farms affected [1]. PRM infestation is associated with severe economic losses in the egg production industry [7], also causing health and welfare issues in the hens [7, 8, 9]. Additionally, the PRM has shown to be a mechanical vector for multiple pathogenic viruses and bacteria [1].
\nThe control of PRM is mainly based on the use of synthetic acaricides. However, synthetic acaricides have important limitations such as the development of resistant mite populations, environmental contamination and limited efficacy for controlling already settled infestations [1, 10]. Thus while research focused on D. gallinae was previously scarce, it increased significantly in recent years probably due to the support received due to the growing impact of mite infestations on the egg-laying industry [10]. The limitations of the conventional control measures make research on alternative control measures as one of the leading research topics in recent years. Amongst those control measures, vaccination poses a promising effective and environmentally sound intervention.
\nThe aim of the present review is to show the current knowledge about the PRM, the challenges it poses from the One Health perspective for both human and animal health and the future possibilities for the control and prevention of PRM infestations.
\n\nDermanyssus gallinae is taxonomically assigned to the Dermanyssidae family englobed in the order Mesostigmata of the Arachnida Class. There are 14 other mite species that affect birds and are morphologically very similar to D. gallinae which may be misidentified when identification is solely based on morphological characteristics [11]. Recent advances in molecular tools as gene sequencing or DNA barcoding, combined with morphological features is allowing a proper mite identification, including D. gallinae identification [12, 13, 14] (Figure 1).
\nSEM images from several morphological characteristics useful for the identification of D. gallinae and differentiation from other similar species. Morphological characteristics shown are present in adult females according to Di Palma et al. [14]. (A) Dorsal overview. Dorsal shield (outline traced) with prominent shoulder. (B) Ventral overview. Epigynal (es) and anal (as) shields are rounded posteriorly. Anal shield with three anal setae (*). (C) Detail of the sternal shield. The sternal shield is wider than long and containing two pairs of setae (*). (D) Detail of dorsal shield. The two pairs of setae (j1 and j2) are on the dorsal shield. See methodology for additional information.
\nDermanyssus gallinae is an obligatory ectoparasite that feeds on the blood of the host. It has a global distribution [15]. In contrast with other Dermanyssus spp., D. gallinae is a generalist species with a low host specificity [16]. The PRM is a pest in the egg-laying farms [1], but can also be found parasitizing more than 30 wild and domestic bird species [11] and mammals [3, 4, 5], including humans [6]. The life cycle for D. gallinae includes five developmental stages: egg, larvae, protonymph, deutonymph and adult (Figure 2). Larvae have three pairs of legs while the rest of the stages have four pairs of legs. The PRM requires a blood meal for molting from protonymph to deutonymph, to adult and for egg-laying [17] (Figure 2). The color of the fed stages varies from bright red to brown, depending on the digestion of the blood inside de mite, while unfed stages are white. Adults and fed deutonymphs are visible with the naked eye. Life cycle usually takes 2 weeks to complete, but it can be shorter when ideal conditions are provided (25–27°C and high relative humidity) [17, 18, 19]. Long-time emptied hen houses have been reported to remain infested. This finding is justified by the ability of the mite to survive without any blood meal for up to 9 months if the environment is suitable. However, desiccation and high temperatures (>45°C) are lethal [19]. Oviposition is carried out only by adult female mites. A maximum of approximately 30 eggs can be laid by a single female in her lifetime, usually in clutches of 4–8 eggs after a blood meal [20].
\nGraphical representation of the PRM biological cycle and points of action for different control measures. Iconography explanation: large red mites = fed adult mites; small red mites = fed nymphal stages; large white mites = starved adults; small white mites = starved nymphal stages; cross = points where the treatment can interrupt the mite cycle; thunderbolt = points of action for the different treatment options. New control interventions such as vaccination, predatory mites or plant extracts are shown. See methodology for information source.
The PRM lacks real eyes and it can senses changes in the luminosity of the environment with photocells [21]. During daylight hours, mite is usually hidden in cracks and crevices where it is out of the reach of the hen. In these shelters, it gathers with more mites until they can form a cluster of hundreds of mites of different stages. This behavior is driven by aggregation pheromones [22]. It is in the darkness when the PRM comes out of their refuges to feed on the host. The host-seeking process is multifactorial, but temperature has been proven to play an important role as the PRM is highly sensitive to even minor changes in temperature and starved mites have an increased sensibility [23, 24]. D. gallinae increases its activity when exposed to substrate vibrations which are supposed to be used for host localization [23]. Surface skin lipids are also involved in the host identification and stimulation [22, 25]. These lipids are used to improve feeding rates in artificial feeding devices when synthetic membranes are used [26] and have provided possibilities for the use of essential oils in the control of PRM infestations in layer houses. In contrast with other hematophagous ectoparasites that utilize CO2 to identify their hosts, CO2 did not induce any host seeking response in D. gallinae under laboratory conditions but induced immobility under light conditions, which is interpreted as a survival strategy to avoid being eaten by the host [23]. Nymphal and adult stages stay on the host for feeding for 30–60 min [27]. According to this behavior, PRM can be considered as a micro predator [16].
\nThe PRM is not a significant issue in the broiler industry, mainly due to its short production cycle, but it poses a substantial threat to the egg-laying industry worldwide, except for layer farms in the USA where Ornythonyssus sylviarum is the main mite species affecting layer hens [28]. However, recent reports suggest significant increase of D. gallinae infestations in the USA [15]. Although O. sylviarum is also present in wild birds of European countries, D. gallinae is the specie responsible for farm infestations. However, mixed infestations have been reported in countries out of Europe [29]. Infestations can reach high prevalence in Europe, where the average prevalence is more than 80% with several countries reaching higher than 90% [30]. However, PRM prevalence can be more related to certain areas rather than a country as different prevalence has been observed in different regions of the same country [31]. PRM infestations have been described in every production system. Less-intensive farming systems present higher risks of infestation which usually is inversely proportional to the level of intensification [32]. Therefore, PRM prevalence is generally higher in backyard and free-range units, followed by barns and, ultimately, by enriched systems [33]. Enriched cages usually show higher levels of infestation when compared to traditional pens in those countries where they are still allowed [32]. These systems improve mite survival by providing more safe areas to the mite far from the reach of the hens and the treatments at the same time as they promote hen welfare.
\nTemporal dynamics of PRM infestations vary greatly between laying hen houses. Specific environmental conditions and differences in laying hen house management are responsible for these variations. The age of the flock is another modulating factor according to a model developed for forecasting the population dynamics in a hen house [34]. The age of the flock has a negative effect on the growth of the mite population as mite populations decline as the age of the hens increases despite the fact that the immune response of the hen against a PRM infestation has not been well characterized. An experimental infestation developed an increase of the serum amyloid-A [35], but hens do not generate natural potent immunoprotective responses [36]. The development of an immune response by the bird after a chronic exposure is a plausible explanation which has been proposed that requires further research [34, 37]. The type of hen hybrid and how they were raised as pullets seem to have some effects on the vertical distribution of the mite infestation in aviaries, which is explained by differences in the space use by different hybrids [38].
\nInfestation levels vary seasonally [38]. Seasons prone to more severe infestations also differ depending on the climate of each region [38]. Usually, seasons with mild temperatures and high relative humidity can be correlated with lower fluctuations of these parameters inside the layer house, providing more ideal conditions for the mite to grow and therefore show more severe infestations. In this way, in northern countries the infestation peak usually happens in summer months while in more temperate climates the most prevalent seasons are spring and autumn.
\nModerate and low infestations do not seem to have an effect on the production parameters independently of the layer hen productive system [39]. Instead, severe infestations are associated with important production losses albeit variations between housing systems [21]. Therefore, PRM has been demonstrated to negatively affect the proportion of laying hens, egg weight and the amount of first-choice eggs in enriched cages facilities while detrimental effects have been observed on egg mass, first-choice eggs and bodyweight of hens housed in aviary systems [39]. The impact on egg production can cause reductions of up to 20% [8]. PRM infestations are also responsible for devaluation of eggs when these are blood spotted. The spots are the result of fed mites getting crushed beneath the eggs while walking or hiding on the conveyor belt [40].
\nPRM is also responsible for health and welfare issues for egg-laying hens. When asked, most egg-producers commonly state that PRM is the major issue concerning hen welfare [41]. The main sign of a severe infestation is the anemia observed in the birds. An adult mite can ingest 0.2 μl of blood in a blood meal [42]. It is described that a laying hen can lose more than 3% of its blood volume every night [8]. In cases of severe infestations, increased bird mortality is observed due to exsanguination. The mortality due to a PRM infestation has been estimated to increase between 4 and 50% [43], and correlates with an increased mite burden. Several studies find significant relationships between PRM infestation and hen mortality [7, 44]. PRM infestation increases food and water intake. Hens under infestation suffer restlessness, agitation, sleep deprivation and increased preening and feather pecking [9, 45]. Thus, the infestation puts the hens into chronic distress making them more susceptible to diseases and reducing vaccine efficacy.
\nMany dermanyssoid mites are confirmed vectors of bacterial and viral pathogens. Several pathogens have been isolated from D. gallinae, thus confirming its role as mechanical vector. Several reports have detected pathogenic bacteria in PRM such as Coxiella burnetii, Erysipelothrix rhusiopathiae, Listeria monocytogenes, Pasterella multocida Mycoplasma gallisepticum, Chlamydophila psittaci and Spirochetes [46, 47, 48]. However, its role as a biological vector for these pathogens is not yet fully elucidated and requires further research. The PRM has been demonstrated under laboratory conditions to act as a vector for Salmonella enteritidis where they showed the oral transmission after ingestion of washed mites contaminated by cuticular contact or during blood meal [49]. Additionally, S. enterica subsp. enterica serovar gallinarum biovar gallinarum (S. gallinarum), the etiological agent of the fowl typhoid, was found to survive for up to 4 months in infected mites [50]. Recently, Pugliese et al. [51] showed the maintenance of S. gallinarum in two different productive cycles where after an outbreak of fowl typhoid, the mites remained infected even after a sanitary break and vaccination of the second flock. An interesting finding of this work was that the number of bacteria found in the mites varied according to the antibody titers of the vaccinated hens. This finding illustrates the complex relationship between host, parasite and bacterial pathogen. PRM has an experimentally confirmed potential capacity for acting as a mechanical vector of avian influenza virus after a bloodmeal on infected hens [52]. Other viral agents such as avipox virus, fowl adenovirus, Marek’s disease virus, avian paramyxovirus type I and the Eastern, Western and Venezuelan equine encephalomyelitis viruses have been isolated from PRM [7].
\nIn summary, PRM is responsible for economic losses of around 231 million Euros annually in Europe considering the combination of the production losses, health issues and cost of mite infestation control [53]. Other reports estimate the economic impact of PRM infestations in Europe between 0.5 and 0.6 Euros per laying hen [54].
\nHistorically, wild birds were considered as the main source of the mite infestation in the poultry houses. However, mitochondrial cytochrome oxidase I (mt-COI) gene sequencing, which allowed secured Dermanyssus species identification, demonstrated that none of the Dermanyssus species that specifically parasitize wild birds were found in poultry farms and concluded that only D. gallinae harbored synanthropic populations [11]. Additionally, the same research described that the D. gallinae populations associated with poultry farms belong to different genetic lineages [11]. In addition, recent research on genetic differences between Ornithonyssus sylviarum present in wild sparrow nests and layer houses in the USA indicated the absence of mite exchange [55]. However, wild bird nests located in the proximities of the hen house can act as a reservoir of mites and thus allow re-infestation. Mul et al. [56] performed a risk analysis in which poultry farmers and employees, followed by hen cadavers and manure aeration, represented the highest risks of introduction and spread of PRM in the farm. If the manure belts are shared amongst barns, they constitute a severe risk of spreading the PRM [56]. Rodents and insects are potential carriers of mites, and although the role of pests in the introduction and spread of PRM in layer farms has not been fully elucidated, a case of phoresy of D. gallinae has been described in a beetle [57].
\nIn a recent questionnaire by free-range farmers in the UK, antiparasitics were reported as one of the three most commonly used medicines against PRM [41]. A recent scandal on the discovery of an unauthorized product in food-producing animals (Fipronil, C12H4Cl2F6N4OS) in contaminated eggs from farms in 45 countries worldwide. The concentration in the contaminated product did not reach toxic doses for humans, but a mediatic Public Health alert was raised, and a food fraud investigation was started by European authorities [58]. Only two compounds are specifically labeled to control PRM infestations while birds are present (Phoxim, C12H15N2O3PS and Spinosad, C41H65NO10 (A); C42H67NO10 (D)) by the European Union (EU), and recently a new compound (Fluralaner, C22H17Cl2F6N3O3) has been approved [59, 60]. Authorized products do not penetrate the whole egg but improper handling when breaking the shell can lead to food contamination [61]. Risks of residues of traditional and unlabeled pesticides entering the food chain are due to its presence in body tissues of hens that are slaughtered for human consumption [60]. A withdrawal period has been suggested for the skin tissue after application of Spinosad and Abamectin (C48H72O14 (B1a); C47H70O14 (B1b)), an acaricide with available formulations for spray application in some European countries, due to the detection of residues in this tissue [62]. The chemicals used to control PRM may also have adverse effects for workers directly exposed while applying the treatment. The limited availability of tools and the increase of resistance are forcing the farmers to turn to non-authorized products to face PRM infestations and underline the necessity for alternative control methods.
\n\nD. gallinae is known as a bird ectoparasite but it has low host specificity [16]. This lack of specificity allows the mite to feed on mammals, including humans, when the natural host is not available [6]. Human parasitosis due to PRM is called gamasoidosis or dermanyssosis. Skin erythematous papules are the usual clinical signs for gamasoidosis and urticarial lesions have been also described [6]. Skin lesions are usually pruriginous and can be distributed throughout the entire body, but are more frequently located in the arms, legs and the upper trunk [6]. Regarding human gamasoidosis associated with D. gallinae, two epidemiological scenarios are described: urban cases and occupational cases [6]. D. gallinae is the most commonly ectoparasite identified as the causal agent of gamasoidosis, but the cases assigned to D. gallinae can be misdiagnosed due to the difficulty of species determination for non-trained practitioners. The geographical expansion of other similar mite species such as Ornythonyssus spp. [63] due to climate change, host expansion and globalization will require more precise analysis.
\nOccupational cases are those related to poultry workers. The infestation can occur both in professional workers and hobbyists. These mite attacks usually happen during the daytime, while the workers are handling birds, cages or collecting eggs or when cleaning the premises. High levels of mite infestation and lack of proper protective clothing increases the risk of mite bites. Despite the high prevalence of infestation in egg-laying farms and continued exposure of the workers to the PRM, the number of reports of occupational cases is limited [6, 64]. The low number of reported cases can be explained by the fact that the attacks occur under specific conditions (severe infestation and lack of protection) or because workers do not report the attacks.
\nUrban cases are not associated with poultry workers. These cases are usually linked to familiar homes or public buildings such as hospitals and halls. In these cases, synanthropic birds, generally pigeons, are the source of the infestation [6]. Most of them occur when the host has left the nest after the breeding season. At that moment, the mites search for a new host to obtain a bloodmeal. Recent investigations suggest the existence of a pigeon specific lineage (D. gallinae L1) that is more frequently involved in human gamasoidosis [65]. Skin lesions in urban cases tend to be more severe than those in occupational cases, basically due to extended exposure.
\nReports of gamasoidosis are scarce but their frequency has increased in the recent years [6]. PRM gamasoidosis is still an underdiagnosed parasitosis mainly due to un-specific signs which do not lead the practitioners to a certain diagnosis and, generally, the fact that PRM bites cause only light to mild clinical symptoms, indistinguishable from other bug bites and do not put the patient in need of seeking medical assistance. Recently, the bacterial genera Tsukamurella has been identified as part of the microbiome of the PRM with an endosymbiotic relationship suggested [66]. Tsukamurella species are foremost saprophyte bacteria that have occasionally been identified as opportunistic organisms associated with postoperative infections [67]. This, and the avian pathogens listed earlier, together with reports of D. gallinae infestations in hospitals [68] highlight potential zoonotic risks associated with PRM. Thus, because of the potential vector role of PRM for zoonotic pathogens it should be included in routine medical differential diagnosis for skin lesions.
\nTreatment and control of PRM infestations have until recently relied on the spraying of chemical acaricides in infested premises, and mostly still occurs despite the limited list of products licensed to be used against the PRM in the EU. In general, traditional control actions achieve only temporary effects and mite populations return to levels prior to treatment soon after treatment application. One of the main limitations in the use of pesticides is the incapacity to apply the product to a degree that does not allow the target to escape from exposure by hiding in cracks and crevices [38]. Another significant problem in the use of pesticides is the emergence of resistances [69]. The number of PRM populations with reduced sensibility to traditional pesticides as λ-Cyhalothrin or Amitraz has grown especially after 2012. In the case of Phoxim, which has been considered a highly effective compound, highly resistant populations have been detected since 2015 [70]. This is probably related to withdrawal of most of the labeled compounds from the marked and subsequent overuse and misuse of the only remaining products available. The single chemical pesticide that shows satisfactory results is a recent labeled to be used as poultry isoxazoline, Fluralaner. Fluralaner has demonstrated a nearly 100% efficacy after two applications in poultry farms [71]. The key for this product is that with the oral administration the treatment reaches the whole mite population when the mites feed on the hens. This delivery method avoids the necessity to spray the product, a way of administration that has been proven of low efficacy for the control of PRM as there are mites that escape from the treatment.
\nAn often-neglected tool for the control of PRM infestations in a layer hen house is the monitorization of the population. Many treatments do not show the expected results because they have not been applied at the right moment. The decision for applying treatment is traditionally taken when the farm employees announce a severe infestation, which is usually too late to allow successful control [72]. A proper monitorization routine can promote early detection and quantification of the infestation level and thus allowing proper programming of control measures. There are multiple methodologies that can be used for monitorization, including both quantitative and qualitative techniques. A description of the most commonly used monitoring methods has been recently reviewed [73]. Many monitoring systems are based on the placement of traps that emulate the hiding places of the mites and that are checked periodically. In this way, depending on the technique the farmer can obtain an estimate of the mite population in the hen house and/or a trend for the mite population evolution. There appears not be a single best choice for a monitoring method as it depends on the time and resources available in the farm. However, farms with monitoring programs in place can improve their capacity of PRM control [74].
\nDevelopment of new control interventions is currently a priority in PRM research as a consequence of the severe impact of the mite in the egg-laying industry and the scarce resources for its control (Figure 2). Amongst those novel methods, treatments with essential oils and plant extracts have received significant attention. There are many studies on the effects of essential oils against PRM, but variable efficacy is observed [75]. Benefits of plant extracts and essential oils include their low mammalian and bird toxicity and short environmental persistence [75]. Several plant-based products are already commercialized against veterinary pests, and many others are in research phase. Essential oils are traditionally used for their repellence of pest arthropods [75]. The effect of essential oils can be due the influence of a number of volatile organic compounds (VOCs) in the host-recognition process [20]. Recent research found that the odor emitted by the hens can be modified through addition of plant-originated VOCs to the food and that some of those VOCs showed repellent activity against the PRM, making the hens less attractive to the mites [76]. The other approach for the use of plant derived compounds is using its insecticide properties for treating the hen house environment. Amongst those substances, neem oil is receiving special attention from researchers [75, 77]. Neem oil preparations are made of essential oil obtained from an Indian tree (Azadirachta indica) and have shown promising effects in PRM population reductions [77]. A disadvantage of neem oil application is the possible effects of the oily film on the farm installations and eggs, but technological improvements such as reducing the volume of solution or the droplet size can be applied to reduce these adverse effects [77].
\nMite communities constituted by different mite species are able to establish themselves in layer farm buildings, mainly associated with manure [78]. These communities include mite species that are predators of free-living nematodes and arthropods, including mites [78]. Some Hypoaspis species identified in starling nests are considered putative predators of D. gallinae [79] and two mite species are already commercialized to be used in layer farms: Androlaelaps casalis (Androlis, APPI-group Koppert. France) and Cheyletus eruditus (Taurrus, APPI-group Koppert. France). A. casalis have shown to control, but no to eradicate, PRM populations under laboratory conditions but was more efficient at temperatures under 30°C [80]. The authors suggested that predation can occur over other mite species when D. gallinae is hiding in safe places, basically at different heights (D. gallinae was on high areas of the cages while predators remained on the floor) [80]. Predatory mites are already effectively used in the control of phytophagous mites in greenhouses and in pig farms for the control of non-hematophagous arthropods. Biocontrol of PRM in layer farmhouses is based upon the massive release of predatory mites. The effectivity of predatory mites to control PRM infestations is variable, probably due to variations in environmental conditions [79]. The main disadvantage of using predatory mites as a control tool of D. gallinae is their high sensitivity to acaricides used to treat PRM infestations [78]. Thus, biocontrol using predatory mites is not compatible with the use of acaricides.
\nAnother control method is based upon a perch design (Q-perch), which prevents the mite from reaching the hens by an electrified wire placed just beneath the perch where the bird is roosting [81]. Various desiccant dusts, diatomaceous earth and synthetic silica products are commonly used in commercial layer farms [74]. Generally, it is a measure used as a temporal constraint of PRM infestation and to reduce the number of treatments with synthetic acaricides. Inert dust kills the mite by dehydration and probably, by cuticle damage by destroying its protective wax layer [82]. The main limitation of the use of inert dusts is the limited efficacy in environments with high levels of relative humidity [82]. A synergistic effect between inert dusts and entomopathogenic fungi have been described [83]. The use of entomopathogenic fungus for the control of PRM is recent and there is limited research. Laboratory tests show promising results, and some have been tested with some success in field trials [84].
\nVaccination against ectoparasites is not solely focused on the prevention of the infestation but also on the reduction of the parasite population [85]. Vaccination have demonstrated to provide high levels of protection against blood-feeding ectoparasites by reducing cattle tick populations and prevalence of certain tick-borne pathogens [86]. The only commercial vaccines against ectoparasites (TickGard and Gavac) were developed with recombinant tick midgut antigens Bm86 and Bm95 and registered for the control of cattle tick infestations [87]. This vaccines demonstrated their efficacy for the control of tick infestations while reducing the use of acaracides and encourage further research for the identification of new effective protective antigens using different approaches [88].
\nVaccine development relies on the identification of proteins that can act as protective antigens to which the host develops an immune response. The identification of protective antigens in D. gallinae has been limited by the lack of molecular research about the mite. The description on the mite transcriptome [89] and, more recently, its genome [90] can enhance the understanding of the host–parasite relationship and the identification of protective antigens. Two approaches have been followed for PRM vaccines development, testing of mite extracts and the production of vaccines based on recombinant proteins (Table 1). Vaccination against PRM recombinant proteins has induced antigen specific IgY responses but variable results have been obtained when mites fed in in vitro tests on blood from immunized hens or blood enriched with antibodies extracted from egg yolk. Another limitation for the assessment of efficacy of a candidate antigen has been the high background effects observed in the in vitro tests due to the feeding physiology of the PRM. A recent optimization of an on-hen feeding device allows a more physiological evaluation of the vaccine effects allowing a better assessment of novel antigens [91]. Vaccines can be considered as an alternative and complementary intervention for PRM control, which can reduce the use of acaricides.
\nAntigen | \nType | \nSpecies | \nAdjuvant | \nTest | \nEffects (%) | \nReference | \n
---|---|---|---|---|---|---|
Soluble protein mite extract | \nNative | \n\nD. gallinae\n | \nIncomplete Freund’s | \nIn vivo [92] | \n↑ 0.1 M | \n[92] | \n
Soluble protein mite extract | \nQuilA | \nIn vitro [93] | \n↑ 24 M | \n[94] | \n||
IEX Group 4 | \n↑ 23.5 M*\n | \n[94] | \n||||
IEX Group 5 | \n↑ 11.4 M*\n | \n[94] | \n||||
IEX Group 2 | \n↓ 4.2 M | \n[94] | \n||||
IEX Group 1 | \n↑ 19.5 M*\n | \n[94] | \n||||
IEX Group 3 | \n↑ 13 M*\n | \n[94] | \n||||
PBS soluble mite extract | \n↑ 10.1 M*\n | \n[93] | \n||||
Membrane associated | \n↑ 2.2 M | \n[93] | \n||||
Urea soluble | \n↑ 0.2 M | \n[93] | \n||||
Integral membrane | \n↓ 1.5 M | \n[93] | \n||||
Mite extract | \nISA 50 V | \nIn vitro [95] | \n↑ 50.7 M*\n | \n[95] | \n||
Soluble protein mite extract | \nISA 207 VG | \nField | \n↓ 78 Pop*\n | \n[96] | \n||
Akirin | \nRecombinant | \n\nAedes albopictus\n | \nISA 50 V | \nIn vitro [95] | \n↑ 35.1 M*\n | \n[97] | \n
Bm86 | \n\nRhipicephalus microplus\n | \n↑ 23 M*\n | \n[97] | \n|||
Histamine release factor | \n\nD. gallinae\n | \nQuilA | \nIn vitro [98] | \n↑ 4.1 M*\n | \n[99] | \n|
Cathepsin D-1 | \nIn vitro [93] | \n↑ 6.9 M*\n | \n[100] | \n|||
Cathepsin L-1 | \n↑ 2.6 M*\n | \n[100] | \n||||
Unknown function protein 1 | \n↑ 18.4 M*\n | \n[94] | \n||||
Unknown function protein 2 | \n↑ 0.6 M | \n[94] | \n||||
Aspartyl proteinase | \n↑ 5.6 M | \n[94] | \n||||
Phosphoglycerate dehydrogenase | \nRecombinant | \n\nD. gallinae\n | \nQuilA | \nIn vitro [93] | \n↑ 4.1 M | \n[94] | \n
Serpin-1 | \n↑ 12 M*\n | \n[94] | \n||||
Hemelipoglycoprotein-1 | \n↑ 18.9 M *\n | \n[94] | \n||||
Vitellogenin-1 | \n↑ 21.9 M*\n | \n[94] | \n||||
Peptidase C1A-like cysteine proteinase | \n↑ 14.5 M | \n[94] | \n||||
Serpin-2 | \n↓ 8.2 M | \n[94] | \n||||
Unknown function protein 3 | \n↑ 3.5 M | \n[94] | \n||||
Paramyosin | \n↑ 20.1 M*\n | \n[101] | \n||||
Tropomyosin | \n↑16.5 M*\n | \n[101] | \n||||
Deg-SRP-1 + Deg-VIT-1 + Deg-PUF-1 | \nISA 70 VG | \nField | \n— | \n[96] | \n||
Calumenin | \nISA 71 VG | \nOn hen [79] | \n↓ 35 O*\n | \n[102] | \n||
Akirin | \n↓ 42 O*\n | \n[103] | \n||||
Cathepsin D-1 | \n↓ 50 O*\n | \n[104] | \n||||
Subolesin | \n\nRhipicephalus microplus\n | \n↓ 44 O*\n | \n[102] | \n|||
Cathepsin D-1 | \nDNA | \n\nD. gallinae\n | \nchicken IL-21 | \n\n | — | \n[104] | \n
Cathepsin D-1 | \n\nEimeria tenella\n | \n\n | — | \n[104] | \n
Antigens tested as vaccine candidates against infestations by D. gallinae.\n
The effects are statistically significant.
Abbreviations: M, mortality; O, Oviposition; ↑, increase; ↓, reduction.
The negative impact of the PRM infestations have become more relevant with recent changes in the production systems, and it is expected to become worse as the market demands more welfare focused systems that reduce the options for controlling poultry infestations. These changes in the production procedures should include increased concerns in biosecurity and monitorization in order to achieve a better understanding of the mite ecology on each farm. PRM infestations constitute a challenge for the modern industry to guarantee hen welfare and prevention of risks for the workers.
\nOmics are a promising tool for enhancing the understanding of the mite-host interactions. These techniques are needed to resolve questions that are yet to be answered such as the determination of the role of the PRM as biological vectors for both poultry and human pathogens and the different mechanisms involved in the immune response in hens or if there are any on the mite side to modulate its host response. Alternative control methods and particularly vaccine are urgently needed for the effective and sustainable control of PRM infestations with the optimization and combination of different interventions.
\nSee methodology for bibliometric analysis.
\nA bibliometric analysis was performed in the web database Scopus (
Images obtained by scanning electron microscope (SEM) were used in Figure 1 to show morphological characters that are useful for species identification [14]. The adult female mite used for SEM photography was dehydrated in absolute ethanol for 24 h. Specimens were mounted onto standard aluminum SEM stubs using conductive carbon adhesive tabs. Mites were observed and photographed with a field emission scanning electron microscope (Zeiss GeminiSEM 500, Oberkochen, Germany) operating in high vacuum mode at an accelerating voltage of 2 kV in the absence of metallic coating.
\nJFLB was supported by Ministerio de Ciencia, Innovación y Universidades (Spain), Doctorado Industrial contract (DI-14-06917) and Sabiotec SA. MV was supported by the University of Castilla- La Mancha (Spain).
\nRecently, energy has become a basic need for modern society. The need of using energy was increased due to population and consumption growth and because the community used various kinds of equipment in supporting convenience in life [1]. The current global energy supply is highly dependent on fossil sources (crude oil, lignite, hard coal, natural gas). These are fossilized remains of dead plants and animals, which have been exposed to heat and pressure in the Earth’s crust over hundreds of millions of years. For this reason, fossil fuels are nonrenewable resources in which reserves are being depleted much faster than the new ones being formed [2].
\nIndonesia as a tropical country has abundant renewable energy sources as alternative energy to replace fossil energy. One alternative energy is biogas. Biogas is the final gas product of anaerobic digestion/degradation (in an environment without oxygen) by methanogenic bacteria [1]. Biogas is very potential as the latest energy source because its methane (CH4) content itself has a heating value of 50 MJ/kg. Methane (CH4) has one carbon in each chain, which can produce combustion more environmentally friendly than that of the long carbon chain fuels. This matter is caused by the less amount of CO2 produced during short carbon chain fuel combustion [3]. One of the main advantages of biogas production is the ability to transform waste material into a valuable resource, by using it as a substrate for AD [2].
\nAnaerobic digestion (AD) has been extensively used to convert organic waste streams from various sources, such as agricultural, industrial, and municipal solid waste, to biogas. The AD process can operate in both liquid and solid states in terms of total solid (TS) content. In general, the TS content of liquid AD (L-AD) systems ranges from 0.5 to 15%, while solid-state AD (SS-AD) systems usually operate at TS contents of higher than 15% [4].
\nAnaerobic digestion (AD) relies on efficient conversion of organic matter into a valuable product known as biogas, with methane (CH4) as its main combustible constituent. The biogas can be used as energy for household cooking, lighting, heating, and other applications. The process is heavily dependent upon the mutual and syntrophic interaction of a consortium of microorganisms to break down the complex organic matter into soluble monomers such as amino acids, fatty acids, simple sugars, and glycerols. For AD process optimization, it is vital to understand these biological processes and their associated chemical reactions [5].
\nThere are four basic stages involved in AD. These four basic stages make up the process of biogas production from various organic materials as it occurs in an anaerobic digester. These four stages are the hydrolysis, acidogenesis, acetogenesis, and methanogenesis. The AD process is characterized by the decomposition of organic matter into methane, carbon dioxide, inorganic nutrients, and compost in an anaerobic environment [6].
\nMany different types of anaerobic digesters are available. These vary in configuration, retention time, pre- and posttreatment requirements, and operating temperature, among other things, depending upon the principal feedstocks being treated. During AD, the breakdown of organic compounds is achieved by a combination of many types of bacteria and archaea (microbes). The biomass added to the digester is broken down into sugars, amino acids, and fatty acids (hydrolysis), fermented to produce volatile fatty acids and alcohols (acidogenesis) followed by the conversion into hydrogen, carbon dioxide, and ammonia. In addition, methanogens produce biogas from acetic acid and hydrogen [7].
\nThe addition of biostarter can maximize biogas production. The selection of a good starter is very important to speed up the process overhaul of organic matter. Rumen fluid can be used as a good biostarter because in it there are cellulolytic and methanogenic bacteria. Cellulolytic bacteria degrade an organic material to become a substrate of methanogenic bacteria [8]. The addition of rumen fluid can also shorten the time to reach peak production of methane gas compared to substrates that are not given rumen fluid [9].
\nWater hyacinth (Eichhornia crassipes) is a water plant that grows in swamps, lakes, reservoirs, and rivers and that flows calmly. The leaves of the water hyacinth are bright green, have an ovate shape, and widen with a diameter of up to 15 cm [10]. The water hyacinth (Eichhornia crassipes) is generally considered as a waterweed, which has become a problem that damages the environment, the system irrigation, and agriculture [11]. Water hyacinth is a type of weed that grows very fast. The growth of water hyacinth can reach 1.9% per day with a height between 0.3 and 0.5 m. Its rapid growth is felt to be very detrimental because water hyacinth plants that covered the surface of the water will cause the oxygen content to decrease [12].
\nWater hyacinth has attracted attention to scientists to use it as a potential biomass because its rich in nitrogen, essential nutrients, and high fermentation contents [13].
\nIn Indonesia, most of the major lakes are also facing environmental problems such as eutrophication, sedimentation, and a decline in surface area. Indonesia has determined that 15 lakes have become a national priority to be restored and preserved [14]. Behind its beauty, Rawa Pening Lake keeps a pile of concerns. The 2667 hectare natural reservoir located in Ambarawa, Bawen, Tuntang, and Banyubiru, Semarang Regency, is currently being staked out by sedimentation, not to mention the uncontrolled growth of water hyacinth that takes up lake land. The decline in water storage capacity due to the sedimentation process results in a decrease in reservoir function and effectiveness. Rawa Pening Lake has even been included in the list of 15 critical lakes in Indonesia [15].
\nRawa Pening Lake has been facing an invasion of macrophytes indicated by a massive growth of water hyacinth that covers more than 40% of the lake surface [16]. Although the water hyacinth is often used, it does not reduced. Their growth is so fast causing water hyacinth plants become into waterweeds. Water hyacinth is being utilized as a biogas raw material because it has carbohydrate and cellulose contents. Cellulose will be hydrolyzed into glucose by bacteria which will produce methane gas as biogas [10]. An image of a massive growth of water hyacinth in Rawa Pening Lake, Indonesia, is shown in Figure 1.
\nA massive growth of water hyacinth in Rawa Pening Lake, Central Java, Indonesia.
Biogas contains methane, and it is the combustion of methane which constitutes the energy component of biogas [7]. It consists mainly of methane (CH4) and carbon dioxide (CO2) and is formed from the anaerobic bacterial decomposition of organic compounds, i.e., without oxygen. The gases formed are the waste products of the respiration of these decomposer microorganisms, and the composition of the gases depends on the substance that is being decomposed. If the material consists of mainly carbohydrates, such as glucose and other simple sugars and high molecular compounds (polymers) such as cellulose and hemicellulose, the methane production is low. However, if the fat content is high, the methane production is likewise high [17].
\nBiogas may be used in many different ways:
Combusted directly in domestic stoves for cooking or used in gas lamps for lighting
After minor treatment, combusted in boilers to generate heat, internal or external combustion engines to produce electricity, combined heat and power (CHP) plants to produce both heat and electricity, and tri-generation systems to provide cooling via absorption chillers in addition to heat and electricity
Upgraded into biomethane to be used as vehicle fuel in gas-powered vehicles; to be used in place of natural gas in industrial, commercial, and domestic uses; or to be pumped into gas grids to substitute natural gas supplied to households and businesses [7].
There were a lot of researches about biogas production that used various parameters that effected to it. These were food to microorganism (F/M) ratio, carbon to nitrogen (C/N) ratio, and total solid (TS). In the production of biogas from anaerobic digestion, the value of the food to microorganism (F/M) ratio shows the ratio between the mass of food available in the substrate and the mass of microorganisms that act as decomposers. A food to microorganism (F/M) ratio that is too small can cause microbes to be not metabolized completely, and if the value of the F/M ratio is excessive, it results in an unbalanced metabolism [18].
\nIn addition to the organic content of the substrate, the carbon to nitrogen (C/N) ratio was stated as an important factor for the biogas process. The C/N ratio should be in the range between 10 and 30 and, as an optimal ratio, between 25 and 30 for digesters operating at full potential. When the C/N ratio is low, there is a risk of ammonia obstruction, the process of methanogenesis being more sensitive. High ratios can lead to low methane yields equivalent to the lack of nitrogen available for cell growth [19].
\nAccording to Brown and Li (2013) in the production of biogas from biomass raw materials, lignocellulose is appropriate to be produced from using the SS-AD method because lignocellulosic biomass has a total solid concentration of >15% and has low moisture content. According to Malik (2006) water hyacinth contains 95% water and consists of networks that are hollow, and this is the reason why L-AD method is well applied to water hyacinth because of its TS content which is relatively low [20].
\nSome researches about biogas production of water hyacinth have been done by students of the Environmental Engineering Diponegoro University. The researches were about biogas production from water hyacinth using liquid anaerobic digestion (L-AD) and solid-state anaerobic digestion (SS-AD). The part that was used from water hyacinth was the leaves.
\nPreliminary methods were conducted before doing the main researches to know about the contents of each component that will be used. Various parameters will be used in biogas researches.
\nAccording to the American Public Health Association (APHA) standard method, the formula for total solid content can be seen in Eq. (1):
\nDescription: W1, cup weight; W2, cup weight and sample weight; W3, cup weight and sample weight after being ovened.
\nThe procedure carried out in this test was taken from several references, namely, Black (1965); Graham (1948); Page et al. (1982); Rayment et al. (1992) in Sulaeman et al. (2005) “Technical Guidelines for Soil, Plant, Water, and Chemical Chemical Analysis of Soil Research Institute Indonesian Ministry of Agriculture.” With the following method of work, 0.500 g soil of size <0.5 mm was weighed and put in a 100 ml volumetric flask. 5 ml of K2Cr2O7 1 N was added and then mixed. 7.5 ml of concentrated H2SO4 was added, mixed, and let to sit for 30 min. Diluted with ion free water, the mixture was allowed to cool and squeeze. In the next day, absorbance of the clear solution was measured with a spectrophotometer at a wavelength of 561 nm. As a comparison standard, 0 and 250 ppm were made, by piping 0 and 5 ml of the 5000 ppm standard solution into a 100 ml volumetric flask with the same treatment as the working sample [21].
\nIn this test, the procedure was taken from several references, namely, Black, (1965); Page et al. (1982); Burt (2004); and Lisle et al. (1990) in Sulaeman et al. (2005) “Technical Guidelines for Soil, Plant Chemical Analysis, Water and Fertilizers, Indonesian Ministry of Agriculture Soil Research Institute.” This test was divided into two stages: the destruction stage and the measurement stage [21].
\nSamples that had been researched in preliminary methods could be inserted into the reactor and mixed with other components that are related; then the reactor must be sealed in order to obtain anaerobic digestion. During the treatment process, the volume of biogas production was observed in an interval of 2 days throughout 60 days.
\nTo find out the amount of biogas, place the reversed cylinder glass in the container that is filled with water (reversed cylinder glass must be filled with water). Place the plastic tube into the reversed cylinder glass. Record the initial volume from it. Open the clip that clipped the plastic tube (the clip’s function was to avoid the oxygen entered into the digester). The biogas will go out through the plastic tube and will make the water volume to decrease. Record the final volume. Lastly, record the biogas volume by counting the water level difference. The digester is shown in Figure 2.
\nSchematic diagram of series laboratory batch assessment of L-AD and SS-AD [23].
TS content of liquid anaerobic digestion (L-AD) systems ranges from 0.5 to 15% [4]. The research about “Biogas Production from Water Hyacinth (Eichhornia crassipes): The Effect of F/M Ratio” [22] was conducted to know about the effect of F/M ratio to biogas production from water hyacinth leaves using the liquid anaerobic digestion (L-AD) method. In biogas production anaerobically, the value of F/M shows a comparison between the amount of substrate that is contained in waste (medium) and the amount of microorganism used [18]. The variation of F/M ratio depends on the existence of rumen volume variation and total solid from each materials.
\nThe main substrate used in the research [22] was water hyacinth leaves as much as 200 g. The initial total solid of water hyacinth leaves that has been calculated using (Eq. (1)) was 13.52. When it is combined with a different cow rumen fluid volume in each reactor, the total solid of water hyacinth leaves will be changed. To find out the F/M ratio, a comparison of the total solid of water hyacinth leaves with cow rumen fluid was multiplied by the weight and volume of each ingredient. The data is shown in Table 1.
\nF/M ratio | \nInitial total solid (%) | \nCow rumen fluid volume (ml) | \nFinal total solid (%) | \n
---|---|---|---|
39.76 | \n13.52 | \n50 | \n10.82 | \n
20.03 | \n13.52 | \n100 | \n9.06 | \n
13.32 | \n13.52 | \n150 | \n7.73 | \n
10.01 | \n13.52 | \n200 | \n6.76 | \n
Initial and final total solid of water hyacinth leaves.
After the research had been done, results show that the biogas production with F/M ratio of 10.01 and TS of 6.76% produced more biogas in the amount of 127.071 ml/g TS. Figure 3 shows the graphic of cumulative biogas yield.
\nCumulative biogas yield per g TS based on F/M ratio.
A research has also been conducted [20] using water hyacinth leaves as much as 200 g as the main substrate combined with water and rumen fluid. The combination is shown in Table 2.
\nInitial total solid (%) | \nCow rumen fluid volume (ml) | \nWater volume (ml) | \nFinal total solid (%) | \n
---|---|---|---|
13.52 | \n150 | \n— | \n6.76 | \n
13.52 | \n150 | \n300 | \n3.38 | \n
Initial and final total solid of water hyacinth leaves.
In this study to get a low total solid content, rumen was added to the first variable, and water and rumen were added to the second variable. This is consistent with the research conducted by Astuti (2013) which states that the stuffing material must contain about 6–9% dry matter. This situation can be achieved by dilution [20]. From the graphic below, the final result of biogas production with a TS variable of 6.76% was 177.33 ml/g TS and for a TS variable of 3.38% was 369 ml/g TS. The graphic of cumulative biogas yield/TS is shown in Figure 4.
\nCumulative biogas yield per gram TS.
In addition to total solid and F/M ratio, biogas production is also affected by carbon to nitrogen (C/N) ratio. Various C/N ratio researches have been done [23] by adding organic compound that contained high nitrogen such as urea. In the variation of N elements, C/N ratios of 20, 25, 30, and 35 were produced. The material components of the variables are shown in Table 3.
\nVariable | \nCow rumen fluid volume (ml) | \nWater hyacinth leaves (g) | \nUrea (g)/C/N ratio | \n|||
---|---|---|---|---|---|---|
20 | \n25 | \n30 | \n35 | \n|||
1 | \n200 | \n200 | \n4,9 | \n\n | \n | \n |
2 | \n200 | \n200 | \n4,9 | \n\n | \n | \n |
3 | \n200 | \n200 | \n\n | 3,4 | \n\n | \n |
4 | \n200 | \n200 | \n\n | 3,4 | \n\n | \n |
5 | \n200 | \n200 | \n\n | \n | 3,0 | \n\n |
6 | \n200 | \n200 | \n\n | \n | 3,0 | \n\n |
7 | \n200 | \n200 | \n\n | \n | \n | 2,5 | \n
8 | \n200 | \n200 | \n\n | \n | \n | 2,5 | \n
Research material needs.
The equation for C/N ratio had a real influence on the production of biogas with water hyacinth leaves as a raw material. A variation of C/N ratio of 30 gave the best rate of biogas production among other C/N ratio variables, with biogas yield generated at 191,423 ml/g TS [23]. The result of biogas cumulative yield with C/N ratio can be seen in Figure 5.
\nCumulative biogas yield per g TS based on C/N ratio.
Solid-state anaerobic digestion (SS-AD) systems usually operate at TS contents of higher than 15% [4]. A research has been conducted [20] about the effect of solid-state anaerobic digestion (SS-AD) on biogas production using water hyacinth leaves. In this study to make the total solid content increased, drying method was used. The water hyacinth leaves from Rawa Pening Lake have an initial total solid content of 13.52%. The first variable was dried for 2 days, and the second variable was dried for 1 day. After that, the water hyacinth leaves that have been dried in the sun were examined for their total solid content using Eq. (1). For the first variable, the total solid content was 48.26% and for the second variable was 36.36%. After that the water hyacinth leaves that have been dried in the sun were added to the cow rumen in a ratio of 1:1. In studies using the SS-AD method, no additional water was given [20]. The combination of the variables is shown in Table 4.
\nInitial TS (%) | \nFinal TS after being dried in the sun (%) | \nCow rumen fluid (ml) | \nFinal TS (%) | \n
---|---|---|---|
13.52 | \n48.26 | \n150 | \n24.13 | \n
13.52 | \n36.36 | \n150 | \n17.67 | \n
Initial and final total solid of water hyacinth leaves.
The variable with TS of 24.34% produced biogas with a total of 34.79 ml/g TS, and the variable with TS of 17.67% obtained 52.98 m/g TS. The result is shown in Figure 6.
\nCumulative biogas yield per gram TS.
Further research had been conducted [24] to know about the optimization of total solid (TS) and carbon to nitrogen (C/N) ratio of biogas production from water hyacinth leaves by adding microbial consortium as much as 3%, 6%, and 9%. Meanwhile the total solid contents from the research were 15%, 27.5%, and 40%. And the C/N ratios were 20, 35, and 50. To get the optimum conditions, calculation had been done by the central composite design method with the following variables in Table 5.
\nParameter | \n−1.682 | \n−1 | \n0 | \n+1 | \n+1.682 | \n
---|---|---|---|---|---|
Microbial consortium (%) | \n0.7085 | \n3 | \n6 | \n9 | \n11.2915 | \n
C/N ratio | \n8.54249 | \n20 | \n35 | \n50 | \n61.45751 | \n
Total solid (%) | \n5.45207 | \n15 | \n27.5 | \n40 | \n49.54793 | \n
Variable values in the central composite design.
Variations of variable values in each reactor were obtained using Statistica software as shown in Table 6.
\nReactors | \nTotal solid | \nC/N ratio | \nMicrobial consortium | \n
---|---|---|---|
1 | \n15 | \n20 | \n3 | \n
2 | \n15 | \n20 | \n9 | \n
3 | \n15 | \n50 | \n3 | \n
4 | \n15 | \n50 | \n9 | \n
5 | \n40 | \n20 | \n3 | \n
6 | \n40 | \n20 | \n9 | \n
7 | \n40 | \n50 | \n3 | \n
8 | \n40 | \n50 | \n9 | \n
9 | \n27.5 | \n35 | \n6 | \n
10 | \n5.45207 | \n35 | \n6 | \n
11 | \n49.54793 | \n35 | \n6 | \n
12 | \n27.5 | \n8.54249 | \n6 | \n
13 | \n27.5 | \n61.45751 | \n6 | \n
14 | \n27.5 | \n35 | \n0.70850 | \n
15 | \n27.5 | \n35 | \n11.29150 | \n
16 | \n27.5 | \n35 | \n6 | \n
17 | \n27.5 | \n\n | \n |
Variable values in experiments using central composite design.
In this SS-AD method, variations in the total solid concentration used were 15%, 27.5%, and 40%. The total solid for each reactor was adjusted to the total solid of the water hyacinth. Water and rumen were added to regulate the total solid in each of the reactors. Figures 7–9 show the graphs of biogas results produced at certain reactors which were compared between reactors with the same C/N and microbial consortium ratio values against different TS values [24].
\nEffect of TS on biogas production (Reactors 1 and 5).
Effect of TS on biogas production (Reactors 4 and 8).
Effect of TS on biogas production (Reactors 9, 10, and 11).
The graph in Figure 7 shows the production of biogas produced from Reactor 1 and Reactor 5 where the reactors had the same concentration variations for the same C/N ratio and microbial consortium variables, with the lowest value of each variation of 20 for the C/N ratio and 3% for the concentration of the microbial consortium. The difference was in the total solid concentration (Table 6). Based on the graph in Figure 7, the total cumulative biogas production for Reactor 1 was 27.367 ml/g TS while for Reactor 5 was 5.1 ml/g TS. Reactor 1 with a lower TS which was 15% produced biogas with a greater total than that of the Reactor 5 with TS of 40% [24].
\nReactors 4 and 8 had varying concentrations for the same C/N and microbial consortium variables (Table 6), namely, a C/N ratio of 50 and a microbial consortium concentration of 9%. Both of these variation values are the highest values among the range of values for these variables. The TS concentrations in Reactors 4 and 8 were 15% (lowest value) and 40% (highest value). Reactor 4 with a lower TS value of 15% produces more biogas production than Reactor 8 with a higher TS value (40%). Figure 8 shows that the total biogas production for Reactor 4 was 43.87 ml/g TS and for Reactor 8 was 6.15 ml/g TS [24].
\nThe biogas production graph in Figure 9 came from a reactor with a C/N ratio of 35 and a microbial consortium concentration of 6% (Table 6). This value was the middle value of the variation of concentration for each of these variables. Biogas production varies in each of the reactors. It can be seen in Figure 9 that the total biogas production for Reactors 9, 10, and 11 was 22.65 ml/g TS, 87.85 ml/g TS, and 10.09 ml/g TS. Reactor 10 with TS concentration of 5.45%, C/N ratio of 35, and microbial consortium concentration of 6% produces the largest biogas production when among Reactors 10 and 11 [24].
\nThe variations in the C/N ratio used in this study were 20, 35, and 50. First the C/N ratios of the water hyacinth leaves were tested. To obtain variations in the concentration of the C/N ratio as determined, urea was used to adjust the N value of the water hyacinth leaves [24].
\nDifferent C/N ratios were tested with the same total solid and microbial consortium concentration in Reactor 1 and Reactor 3 (Table 6). Reactor 1 with a C/N ratio of 20, total solid of 15%, and microbial consortium concentration of 3% produced a total biogas of 27.37 ml/g TS. Reactor 3 with a C/N ratio of 50, total solid of 15%, and microbial consortium concentration of 3% produced biogas with a total of 51 ml/g TS. Reactor 3 with a higher C/N ratio of 50 produced more biogas volume than the Reactor 1 with a C/N ratio of 20. The graph is shown in Figure 10 [24].
\nEffect of C/N ratio on biogas production (Reactors 1 and 3).
The graph in Figure 11 was a biogas graph produced from Reactors 6 and 8. The concentrations of the total solid and microbial consortium variables in Reactor 6 were the same as those in Reactor 8 which were 40% and 9%, respectively (Table 6). The C/N ratio of Reactor 6 was 20, while Reactor 8 is 50. For the total biogas production produced, based on the graph in Figure 11, it can be seen that Reactor 6 has a higher biogas than that of the Reactor 8 which was 13.14 ml/g TS for Reactor 6 and 6.15 ml/g TS for Reactor 8. Thus, reactors with lower C/N ratios produce higher biogas under conditions of total solid concentration of 40% and microbial consortium of 9% [24].
\nEffect of C/N ratio on biogas production (Reactors 6 and 8).
The graph in Figure 12 was taken from the calculation of biogas production produced in Reactors 9, 12, and 13. The reactors have the same total solid concentration and microbial consortium (Table 6) of 27.5% and 6%, respectively, with a C/N ratio different from Reactor 9 with a C/N ratio of 35, Reactor 12 with a C/N ratio of 8.54, and Reactor 13 with a C/N ratio of 61.45. The total biogas production in Reactor 9 was 22.65 ml/g TS, whereas in Reactor 12, the total biogas production was 4.76 ml/g TS. For Reactor 13, the total biogas production was 31.24 ml/g TS. The volume of biogas production in Reactor 13 was greater than the volume of biogas production in Reactors 9 and 12 [24].
\nEffect of C/N ratio on biogas production (Reactors 9, 12, and 13).
Water hyacinth was considered as waterweed, which has become a problem that damaged the environment, the system irrigation, and agriculture. Water hyacinth leaves that contained cellulose, nitrogen, essential nutrients, and high fermentation contents can be used for biogas production. The use of the L-AD method with TS 3.38% produced the most biogas yields than using the SS-AD method with TS 24.13 and TS 17.67 or the L-AD method with TS 6.76%, with the amount of biogas yield for TS 3.38% was 369 ml/g TS.
\nBased on the results of research on the effect of the C/N ratio on biogas productivity using L-AD method, the optimum C/N ratio was found in the C/N ratio 30 with the resulting biogas yield of 157.544 ml/g TS. The optimum C/N ratio for biogas production from water hyacinth leaves using the solid-state anaerobic digestion method was 32.09.
\nWe would like to say thank you to the Diponegoro University for the funding of this research under the Research Professorship Program (RPP) (2017).
\nPraise and gratitude for the presence of Allah SWT who has bestowed His mercy and grace so that the author can complete the part of the book with the title Biogas Production from Water Hyacinth chapter. In completing this chapter, we would like to thank the Environmental Engineering Diponegoro University and the water hyacinth biogas research team for their guidance and support. We hope the research that has been done can bring benefits to all people.
\nAD | anaerobic digestion |
biogas | |
C/N | carbon to nitrogen |
cumulative biogas yield | |
F/M | food to microorganism |
Rawa Pening Lake | |
L-AD | liquid anaerobic digestion |
methane | |
SS-AD | solid-state anaerobic digestion |
TS | total solid |
water hyacinth |
The Internet has irrevocably changed the dynamics of scholarly communication and publishing. Consequently, we find it necessary to indicate, unambiguously, our definition of what we consider to be a published scientific work.
",metaTitle:"Prior Publication Policy",metaDescription:"Prior Publication Policy",metaKeywords:null,canonicalURL:"/page/prior-publication-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"A significant number of working papers, early drafts, and similar work in progress are openly shared online between members of the scientific community. It has become common to announce one’s own research on a personal website or a blog to gather comments and suggestions from other researchers. Such works and online postings are, indeed, published in the sense that they are made publicly available. However, this does not mean that if submitted for publication by IntechOpen they are not original works. We differentiate between reviewed and non-reviewed works when determining whether a work is original and has been published in a scholarly sense or not.
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\\n\\n4. SOCIAL MEDIA, BLOG & MESSAGE BOARD POSTINGS
\\n\\nWe feel that social media, blogs and message boards are generally used with the same intention as grey literature, to formulate ideas for a manuscript and gather early feedback from like-minded researchers in order to improve a particular piece of work before submitting it for publication. Therefore, we do not consider such internet postings to be publication in the scholarly sense.
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\\n"}]'},components:[{type:"htmlEditorComponent",content:'A significant number of working papers, early drafts, and similar work in progress are openly shared online between members of the scientific community. It has become common to announce one’s own research on a personal website or a blog to gather comments and suggestions from other researchers. Such works and online postings are, indeed, published in the sense that they are made publicly available. However, this does not mean that if submitted for publication by IntechOpen they are not original works. We differentiate between reviewed and non-reviewed works when determining whether a work is original and has been published in a scholarly sense or not.
\n\nThe significance of Peer Review cannot be overstated when it comes to defining, in our terms, what constitutes a published scientific work. Peer Review is widely considered to be the cornerstone of modern publishing processes and the key value-adding contribution to a scholarly manuscript that a publisher can make.
\n\nOther than the issue of originality, research misconduct is another major issue that all publishers have to address. IntechOpen’s Retraction & Correction Policy and various publication ethics guidelines identify both redundant publication and (self)plagiarism to fall within the definition of research misconduct, thus constituting grounds for rejection or the issue of a Retraction if the work has already been published.
\n\nIn order to facilitate the tracking of a manuscript’s publishing history and its development from its earliest draft to the manuscript submitted, we encourage Authors to disclose any instances of a manuscript’s prior publication, whether it be through a conference presentation, a newspaper article, a working paper publicly available in a repository or a blog post.
\n\nA note to the Academic Editor containing detailed information about a submitted manuscript’s previous public availability is the preferred means of reporting prior publication. This helps us determine if there are any earlier versions of a manuscript that should be disclosed to our readers or if any of those earlier versions should be cited and listed in a manuscript’s references.
\n\nSome basic information about the editorial treatment of different varieties of prior publication is laid out below:
\n\n1. CONFERENCE PAPERS & PRESENTATIONS
\n\nGiven that conference papers and presentations generally pass through some sort of peer or editorial review, we consider them to be published in the accepted scholarly sense, particularly if they are published as a part of conference proceedings.
\n\nAll submitted manuscripts originating from a previously published conference paper must contain at least 50% of new original content to be accepted for review and considered for publication.
\n\nAuthors are required to report any links their manuscript might have with their earlier conference papers and presentations in a note to the Academic Editor, as well as in the manuscript itself. Additionally, Authors should obtain any necessary permissions from the publisher of their conference paper if copyright transfer occurred during the publishing process. Failure to do so may prevent Us from publishing an otherwise worthy work.
\n\n2. NEWSPAPER & MAGAZINE ARTICLES
\n\nNewspaper and magazine articles usually do not pass through any extensive peer or editorial review and we do not consider them to be published in the scholarly sense. Articles appearing in newspapers and magazines rarely possess the depth and structure characteristic of scholarly articles.
\n\nSubmitted manuscripts stemming from a previous newspaper or magazine article will be accepted for review and considered for publication. However, Authors are strongly advised to report any such publication in an accompanying note to the External Editor.
\n\nAs with the conference papers and presentations, Authors should obtain any necessary permissions from the newspaper or magazine that published the work, and indicate that they have done so in a note to the External Editor.
\n\n3. GREY LITERATURE
\n\nWhite papers, working papers, technical reports and all other forms of papers which fall within the scope of the ‘Luxembourg definition’ of grey literature do not pass through any extensive peer or editorial review and we do not consider them to be published in the scholarly sense.
\n\nAlthough such papers are regularly made publicly available via personal websites and institutional repositories, their general purpose is to gather comments and feedback from Authors’ colleagues in order to further improve a manuscript intended for future publication.
\n\nWhen submitting their work, Authors are required to disclose the existence of any publicly available earlier drafts in a note to the Academic Editor. In cases where earlier drafts of the submitted version of the manuscript are publicly available, any overlap between the versions will generally not be considered an instance of self-plagiarism.
\n\n4. SOCIAL MEDIA, BLOG & MESSAGE BOARD POSTINGS
\n\nWe feel that social media, blogs and message boards are generally used with the same intention as grey literature, to formulate ideas for a manuscript and gather early feedback from like-minded researchers in order to improve a particular piece of work before submitting it for publication. Therefore, we do not consider such internet postings to be publication in the scholarly sense.
\n\nNevertheless, Authors are encouraged to disclose the existence of any internet postings in which they outline and describe their research or posted passages of their manuscripts in a note to the Academic Editor. Please note that we will not strictly enforce this request in the same way that we would instructions we consider to be part of our conditions of acceptance for publication. We understand that it may be difficult to keep track of all one’s internet postings in which the researcher´s current work might be mentioned.
\n\nIn cases where there is any overlap between the Author´s submitted manuscript and related internet postings, we will generally not consider it to be an instance of self-plagiarism. This also holds true for any co-Author as well.
\n\nFor more information on this policy please contact permissions@intechopen.com.
\n\nPolicy last updated: 2017-03-20
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