Barely three months into the new year and we are happy to announce a monumental milestone reached - 150 million downloads.
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This achievement solidifies IntechOpen’s place as a pioneer in Open Access publishing and the home to some of the most relevant scientific research available through Open Access.
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We are so proud to have worked with so many bright minds throughout the years who have helped us spread knowledge through the power of Open Access and we look forward to continuing to support some of the greatest thinkers of our day.
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Thank you for making IntechOpen your place of learning, sharing, and discovery, and here’s to 150 million more!
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\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"10722",leadTitle:null,fullTitle:"Pelvic Floor Dysfunction - Symptoms, Causes, and Treatment",title:"Pelvic Floor Dysfunction",subtitle:"Symptoms, Causes, and Treatment",reviewType:"peer-reviewed",abstract:"Pelvic floor disorders (PFDs) refer to a group of conditions, such as urinary incontinence, fecal incontinence, and pelvic organ prolapse, due to weakened or injured pelvic muscles and connective tissues. People with PFDs face several social, mental, and physical health effects due to the bothersome symptoms. In this book, experts and researchers from different countries present the latest evidence in diagnosis and treatment of PFDs. Chapters cover such topics as pelvic floor muscle activity, PFDs and pregnancy and childbirth, non-invasive therapy, dysfunctional voiding in children, and much more.",isbn:"978-1-83969-362-5",printIsbn:"978-1-83969-361-8",pdfIsbn:"978-1-83969-363-2",doi:"10.5772/intechopen.94692",price:119,priceEur:129,priceUsd:155,slug:"pelvic-floor-dysfunction-symptoms-causes-and-treatment",numberOfPages:128,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"fa669d0f9c768ec43040a30b98ca239f",bookSignature:"Ran Pang",publishedDate:"January 19th 2022",coverURL:"https://cdn.intechopen.com/books/images_new/10722.jpg",numberOfDownloads:1105,numberOfWosCitations:0,numberOfCrossrefCitations:0,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:0,numberOfDimensionsCitationsByBook:0,hasAltmetrics:0,numberOfTotalCitations:0,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"April 6th 2021",dateEndSecondStepPublish:"May 4th 2021",dateEndThirdStepPublish:"July 3rd 2021",dateEndFourthStepPublish:"September 21st 2021",dateEndFifthStepPublish:"November 20th 2021",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"186524",title:"Prof.",name:"Ran",middleName:null,surname:"Pang",slug:"ran-pang",fullName:"Ran Pang",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bSB2eQAG/Profile_Picture_1644508746393",biography:"Ran Pang is a consultant urologist and leader in functional urology and urodynamics at Guang’anmen hospital, China Academy of Chinese Medical Sciences. After completing residency training, he was accepted to a clinical fellowship with Peking University in 2005. Subsequently, he joined a research fellowship at Mayo Clinic, USA, in 2011, and a urodynamic fellowship at Dalhousie University, Canada, in 2015. As a leading expert, prof. Pang also serves on several international organizations as well as local professional committees, such as chair of the Publication and Communication Committee, International Continence Society, and vice-chair of the Pelvic Floor Disorder Group of Urology Committee, Chinese Association of Integrative Medicine. Additionally, he received the Albert Nelson Lifetime Achievement award in 2017.",institutionString:"Guang’anmen Hospital",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"4",totalChapterViews:"0",totalEditedBooks:"3",institution:{name:"Guang’anmen Hospital",institutionURL:null,country:{name:"China"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"1071",title:"Urogynecology",slug:"urogynecology"}],chapters:[{id:"79541",title:"Pelvic Floor Disorders in Females: An Overview on Diagnostics and Therapy",doi:"10.5772/intechopen.101260",slug:"pelvic-floor-disorders-in-females-an-overview-on-diagnostics-and-therapy",totalDownloads:90,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Pelvic floor disorders have multifactorial reasons and can have a huge impact on a woman’s life. They can result in descensus of bladder, uterus, vagina or rectum and are often accompanied by incontinence. Symptoms like downward pressure, pain, incontinence or bladder voiding dysfunction develop slowly and are still highly taboo. Gynecology differentiates between descensus of the anterior, central and posterior compartment. A descensus in the anterior compartment causes a cystocele, with can either present as a pulsation cystocele or a traction cystocele. A descensus of the apical compartment leads to a uterine prolapse or vaginal stump descensus, while a descensus of the posterior compartment results in a recto- or enterocele. Urinary incontinence can be divided into stress and urge incontinence. The most important tool for the diagnosis of pelvic floor disorders is the clinical examination. Regarding the therapy of pelvic floor disorders, conservative therapy measures should first be offered. If these fail, an individually optimized surgical therapy should follow. The spectrum of surgical possibilities has expanded considerably in the last three decades. In particular, implanting alloplastic meshes has improved long-term stability. Finally, preventive measures also play a central role.",signatures:"Isabell Link and Christian Fünfgeld",downloadPdfUrl:"/chapter/pdf-download/79541",previewPdfUrl:"/chapter/pdf-preview/79541",authors:[{id:"417461",title:"Dr.",name:"Isabell",surname:"Link",slug:"isabell-link",fullName:"Isabell Link"},{id:"418241",title:"Dr.",name:"Christian",surname:"Fünfgeld",slug:"christian-funfgeld",fullName:"Christian Fünfgeld"}],corrections:null},{id:"77314",title:"Pelvic Floor Muscle Activity in Relation to Body Position and Breath",doi:"10.5772/intechopen.98681",slug:"pelvic-floor-muscle-activity-in-relation-to-body-position-and-breath",totalDownloads:90,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The aim of this work was to analyse pelvic floor muscle activity by intravaginal perineometry. We focused on the increase caused by phasic muscular activity, which, on a short term basis, rises above the basal tonic activity. The functional relationships to postural and respiratory function have been confirmed by only a few studies. Therefore, we monitored this functional connection. We confirmed a statistically significant increase in pelvic floor muscle activity at deep breathing compared to calm breathing (in other words at different breathing intensity) in the same position (lying, standing). Our measurements also showed that the phasic activity of pelvic floor muscles in deep breathing is statistically significantly higher than activity after a minute-long run on the treadmill. Cough is a specific situation, whose short-term increase in pelvic floor muscle activity clearly exceeds all other monitored situations.",signatures:"Monika Sorfova and Eva Tlapakova",downloadPdfUrl:"/chapter/pdf-download/77314",previewPdfUrl:"/chapter/pdf-preview/77314",authors:[{id:"414713",title:"Associate Prof.",name:"Monika",surname:"Sorfova",slug:"monika-sorfova",fullName:"Monika Sorfova"},{id:"414715",title:"Dr.",name:"Eva",surname:"Tlapakova",slug:"eva-tlapakova",fullName:"Eva Tlapakova"}],corrections:null},{id:"78537",title:"Perineal Management and Episiotomy Practice in Chile",doi:"10.5772/intechopen.100033",slug:"perineal-management-and-episiotomy-practice-in-chile",totalDownloads:110,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Episiotomy is a common intervention used during the second stage of delivery. Current use of this procedure is restricted to certain births due to several complications. Almost all births in Chile are delivered by a gynecologist or a midwife in the public or private health system where episiotomy is performed. The objective of this study is to identify strengths and weakness in aspects of perineal management and episiotomy practice among obstetric health care providers with the purpose of promoting practice assessment and updating skills and competencies. Design: Questionnaire-based-cross-sectional study. Method: Anonymous questionnaire applied to gynecologists and midwives of public and private hospitals, between October and December 2019 using the Instrument designed by Cornet et al. addressing questions such as affiliation, number of births/year, knowledge of anatomy, knowledge of episiotomy, knowledge of perineal tear, competence in perineal repair, and presence of expert in perineal trauma at their unit. Results: 189 surveys responded, 51% from midwives and 37.6% from doctors. 71% of total were trained at their medical or midwifery schools and 69% during postgraduate internships. Episiotomy practice criteria: 19% always in primigravida patients and 14,3% always in premature deliveries. Majority of professionals, 79.4% with less than 100 deliveries a year had incorrect answers about depth or sphincter tear prevention technique. Conclusions: The majority of professionals indicated insufficient training capacities in relation to episiotomy techniques. Undergraduate programs should strength training on this intervention, national guidelines must include routine episiotomy performance in order to unify criteria.",signatures:"Cristhel K. Fagerstrom-Sade, Sara Parada-Ibañez, Marcela M. Gurovich, Esther Ayala-Pérez, Pablo Ortega-Plancic, Jaime Roa-Burgos, Fabián Vasquez-Vergara and Jorge Rodriguez-Suazo",downloadPdfUrl:"/chapter/pdf-download/78537",previewPdfUrl:"/chapter/pdf-preview/78537",authors:[{id:"414771",title:"Prof.",name:"Cristhel K.",surname:"Fagerstrom-Sade",slug:"cristhel-k.-fagerstrom-sade",fullName:"Cristhel K. Fagerstrom-Sade"},{id:"427675",title:"Prof.",name:"Marcela M.",surname:"Gurovich",slug:"marcela-m.-gurovich",fullName:"Marcela M. Gurovich"},{id:"427678",title:"Prof.",name:"Sara",surname:"Parada-Ibañez",slug:"sara-parada-ibanez",fullName:"Sara Parada-Ibañez"},{id:"427681",title:"Dr.",name:"Pablo",surname:"Ortega-Plancic",slug:"pablo-ortega-plancic",fullName:"Pablo Ortega-Plancic"},{id:"427682",title:"Dr.",name:"Jaime",surname:"Roa-Burgos",slug:"jaime-roa-burgos",fullName:"Jaime Roa-Burgos"},{id:"427683",title:"Prof.",name:"Jorge",surname:"Rodriguez-Suazo",slug:"jorge-rodriguez-suazo",fullName:"Jorge Rodriguez-Suazo"},{id:"427684",title:"Prof.",name:"Fabian",surname:"Vasquez-Vergara",slug:"fabian-vasquez-vergara",fullName:"Fabian Vasquez-Vergara"},{id:"427685",title:"Dr.",name:"Esther",surname:"Ayala-Pérez",slug:"esther-ayala-perez",fullName:"Esther Ayala-Pérez"}],corrections:null},{id:"78192",title:"Therapeutic Effect of Magnetic Stimulation Therapy on Pelvic Floor Muscle Dysfunction",doi:"10.5772/intechopen.99728",slug:"therapeutic-effect-of-magnetic-stimulation-therapy-on-pelvic-floor-muscle-dysfunction",totalDownloads:311,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Pelvic bottom dysfunction includes sexual dysfunction, lower urinary tract dysfunction, defecation dysfunction, etc., and the quality of daily life is significantly impaired. Although drug based and surgical therapies exist as treatment methods, non-invasive treatment methods for pelvic floor dysfunction are highly desired, and magnetic stimulation therapy is attracting attention as a potential new approach. Magnetic stimulation therapy can generate deeper stimulations as compared to electrical stimulation therapy, is less painful, and can be performed while wearing clothes. In addition, it is a very safe treatment method with only few reports of side effects. From nocturnal enuresis in children to middle-aged sexual dysfunction and urinary incontinence in the elderly, therapeutic effects on various pelvic floor dysfunctions have been confirmed regardless of age and gender. It is expected that magnetic therapy will continue to develop as a new therapy in the futures. This chapter first describes the pelvic floor muscles and the principles of anatomy and magnetic therapy. In addition, the therapeutic effects of magnetic therapy will be explained in detail one by one. We will also explain the potential application of magnetic therapy for sarcopenia, which is a problem in our aging society.",signatures:"Takuro Kobayashi, Toshiyuki China, Naoko Takazawa, Fumitaka Shimizu, Julius Fink, Shigeo Horie and Tomohiro Imai",downloadPdfUrl:"/chapter/pdf-download/78192",previewPdfUrl:"/chapter/pdf-preview/78192",authors:[{id:"416441",title:"Prof.",name:"Shigeo",surname:"Horie",slug:"shigeo-horie",fullName:"Shigeo Horie"},{id:"428848",title:"Dr.",name:"Takuro",surname:"Kobayashi",slug:"takuro-kobayashi",fullName:"Takuro Kobayashi"},{id:"428849",title:"Dr.",name:"Toshiyuki",surname:"China",slug:"toshiyuki-china",fullName:"Toshiyuki China"},{id:"428850",title:"Dr.",name:"Naoko",surname:"Takasawa",slug:"naoko-takasawa",fullName:"Naoko Takasawa"},{id:"428851",title:"Dr.",name:"Fumitaka",surname:"Shimizu",slug:"fumitaka-shimizu",fullName:"Fumitaka Shimizu"},{id:"428852",title:"Dr.",name:"Julius",surname:"Fink",slug:"julius-fink",fullName:"Julius Fink"},{id:"429045",title:"Dr.",name:"Tomohiro",surname:"Imai",slug:"tomohiro-imai",fullName:"Tomohiro Imai"}],corrections:null},{id:"78509",title:"Management of Hunner Lesion in Interstitial Cystitis/Bladder Pain Syndrome Patients",doi:"10.5772/intechopen.100052",slug:"management-of-hunner-lesion-in-interstitial-cystitis-bladder-pain-syndrome-patients",totalDownloads:117,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic condition characterized by chronic pelvic pain related to the bladder. One phenotype of IC/BPS is the Hunner lesion type IC/BPS. Hunner lesion exhibits typical features such as mucosal ulceration, fibrosis, and severe inflammation. The tissue surrounding the Hunner lesion may show lymphoplasmacytic infiltrates, and mast cells are increased in the lamina propria. In this chapter, we discuss intravesical treatment, endoscopic treatment, and partial cystectomy with augmentation cystoplasty for the management of Hunner lesion in IC/BPS patients.",signatures:"Kwang Jin Ko and Kyu-Sung Lee",downloadPdfUrl:"/chapter/pdf-download/78509",previewPdfUrl:"/chapter/pdf-preview/78509",authors:[{id:"307239",title:"Prof.",name:"Kyu-Sung",surname:"Lee",slug:"kyu-sung-lee",fullName:"Kyu-Sung Lee"},{id:"310021",title:"Prof.",name:"Kwang Jin",surname:"Ko",slug:"kwang-jin-ko",fullName:"Kwang Jin Ko"}],corrections:null},{id:"78523",title:"Development of Management Model Post-Stroke Urinary Incontinence",doi:"10.5772/intechopen.99700",slug:"development-of-management-model-post-stroke-urinary-incontinence",totalDownloads:123,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Post-stroke urinary incontinence (UI) is one of the sequelae of stroke. This situation affects all aspects of the patient’s life both physically, psychologically, socially, and spiritually. Post-stroke UI as a chronic disease requires holistic treatment. Many chronic health problems will respond well when handled from a holistic perspective. The holistic health view focuses on the patient’s health care needs, not only needs related to the patient’s physical condition of health, but also subjective aspects related to social representation of his/her health conditions. Developing a holistic post-stroke UI management model and continuous care at the patient’s home needs to be done. This chapter includes model components which are also interventions that can be done to overcome post-stroke UI. These interventions include information and understanding of post-stroke UI; ways to overcome post-stroke UI; conduct self-control and stay motivated; perform daily activities independently according to ability; and get family support and peer attention. This model was developed based on previous qualitative studies and literature studies related to post-stroke urinary incontinence. Intervention in this model is aimed at patients who have passed the acute phase of stroke, when they will be discharge from the hospital and continued at the patient’s home.",signatures:"Heltty Heltty, Ratna Sitorus, Nury Nusdwinuringtyas and Evi Martha",downloadPdfUrl:"/chapter/pdf-download/78523",previewPdfUrl:"/chapter/pdf-preview/78523",authors:[{id:"415244",title:"Associate Prof.",name:"Heltty",surname:"Heltty",slug:"heltty-heltty",fullName:"Heltty Heltty"},{id:"415902",title:"Prof.",name:"Ratna",surname:"Sitorus",slug:"ratna-sitorus",fullName:"Ratna Sitorus"},{id:"415903",title:"Dr.",name:"Nury",surname:"Nusdwinuringtyas",slug:"nury-nusdwinuringtyas",fullName:"Nury Nusdwinuringtyas"},{id:"415904",title:"Dr.",name:"Evi",surname:"Martha",slug:"evi-martha",fullName:"Evi Martha"}],corrections:null},{id:"77192",title:"Rehabilitation Protocols for Children with Dysfunctional Voiding",doi:"10.5772/intechopen.98573",slug:"rehabilitation-protocols-for-children-with-dysfunctional-voiding",totalDownloads:188,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Dysfunctional voiding is a functional voiding disorder characterized by an intermittent uroflow rate due to involuntary intermittent contractions of the striated muscle of the external urethral sphincter or pelvic floor muscles (PFMs) during voiding in neurologically normal children. Symptoms include voiding difficulties as well as urgency, voiding frequency and, in some instances, urinary incontinence and/or nocturnal enuresis. Recurrent urinary tract infections, chronic constipation and/or fecal incontinence and vesicoureteral reflux (VUR) contribute to this condition. Urotherapy is the mainstay of the treatment. It starts with education and demystification and simple behavioral modifications. Specific measures include PFM exercises with various forms of biofeedback concentrating at the recognition of PFM function and their relaxation. However, the PFMs are part of the abdominal capsule and they act in coordination with lower abdominal muscles. These muscles need to be relaxed during voiding. Diaphragmatic breathing exercises were introduced to teach children abdominal muscle relaxation. Easy to learn exercises do not require any specific equipment and can be performed at all health care levels. Children from five years of age could benefit from these exercises. In children resistant to standard treatment, botulinum toxin type A application, intermittent catheterization and surgery in children with VUR are recommended.",signatures:"Vesna D. Zivkovic, Ivona Stankovic, Lidija Dimitrijevic, Hristina Colovic, Dragan Zlatanovic and Natasa Savic",downloadPdfUrl:"/chapter/pdf-download/77192",previewPdfUrl:"/chapter/pdf-preview/77192",authors:[{id:"415674",title:"Associate Prof.",name:"Vesna D.",surname:"Zivkovic",slug:"vesna-d.-zivkovic",fullName:"Vesna D. Zivkovic"},{id:"417784",title:"Prof.",name:"Ivona",surname:"Stankovic",slug:"ivona-stankovic",fullName:"Ivona Stankovic"},{id:"417785",title:"Dr.",name:"Dragan",surname:"Zlatanovic",slug:"dragan-zlatanovic",fullName:"Dragan Zlatanovic"},{id:"420465",title:"Prof.",name:"Lidija",surname:"Dimitrijevic",slug:"lidija-dimitrijevic",fullName:"Lidija Dimitrijevic"},{id:"420466",title:"Prof.",name:"Hristina",surname:"Colovic",slug:"hristina-colovic",fullName:"Hristina Colovic"},{id:"420467",title:"Dr.",name:"Natasa",surname:"Savic",slug:"natasa-savic",fullName:"Natasa Savic"}],corrections:null},{id:"79079",title:"Surgical Treatment of Pelvic Organ Prolapse",doi:"10.5772/intechopen.100232",slug:"surgical-treatment-of-pelvic-organ-prolapse",totalDownloads:76,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"The human being is the only mammal capable of walking and simultaneously maintaining an upright position. This fact, implies somewhat unfavorable repercussions for the pelvic region that must support the weight of the abdominal organs. A prime example of the aforementioned adverse effects of the standing position are pelvic organ prolapses (POP). POP surgery is an increasingly important therapeutic aspect in clinical practice due to the aging of our population, and is increasingly prevalent as a therapeutic option. Surgical techniques can be performed using an abdominal or vaginal approach, depending on the medical history, physical examination, and experience of the surgeon. Laparoscopic sacrocolpopexy is an adequate therapeutic option with a high success rate in 80–100% of cases. However, this technique is not always appropriate, especially for patients who are at high risk for anesthesia, a multi-operated abdomen, or in recurrent prolapse. In these cases, a vaginal approach offers an interesting surgical alternative. In this review, we added our experience with transvaginal single-incision mesh under locoregional anesthesia for correction of female POP. We retrospectively analyzed 78 patients showing a success rate of 92% after more than 12 months of follow up. Transvaginal mesh was developed to maintain the advantage of a vaginal procedure, while reducing the risk of recurrent prolapse compared to native tissue repair and simplifying the surgery compared to sacrocolpopexy.",signatures:"Cristina Margarita Fernández-Ávila, Rodrigo García-Baquero, Ana Victoria Ojeda Claro, Blanca Madurga Patuel and José Luis Álvarez-Ossorio",downloadPdfUrl:"/chapter/pdf-download/79079",previewPdfUrl:"/chapter/pdf-preview/79079",authors:[{id:"185782",title:"Dr.",name:"Rodrigo",surname:"Garcia-Baquero",slug:"rodrigo-garcia-baquero",fullName:"Rodrigo Garcia-Baquero"},{id:"193860",title:"Dr.",name:"Blanca",surname:"Madurga Patuel",slug:"blanca-madurga-patuel",fullName:"Blanca Madurga Patuel"},{id:"194046",title:"Dr.",name:"Jose Luis",surname:"Alvarez-Ossorio",slug:"jose-luis-alvarez-ossorio",fullName:"Jose Luis Alvarez-Ossorio"},{id:"415529",title:"Dr.",name:"Cristina Margarita",surname:"Fernández-Ávila",slug:"cristina-margarita-fernandez-avila",fullName:"Cristina Margarita Fernández-Ávila"},{id:"415531",title:"Dr.",name:"Ana Victoria",surname:"Ojeda Claro",slug:"ana-victoria-ojeda-claro",fullName:"Ana Victoria Ojeda Claro"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"10355",title:"Urinary Tract Infection and Nephropathy",subtitle:"Insights into Potential Relationship",isOpenForSubmission:!1,hash:"ca250be6457e17cd92a4e48ffc32724d",slug:"urinary-tract-infection-and-nephropathy-insights-into-potential-relationship",bookSignature:"Ran Pang",coverURL:"https://cdn.intechopen.com/books/images_new/10355.jpg",editedByType:"Edited by",editors:[{id:"186524",title:"Prof.",name:"Ran",surname:"Pang",slug:"ran-pang",fullName:"Ran Pang"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"7957",title:"Lower Urinary Tract Dysfunction",subtitle:"From Evidence to Clinical Practice",isOpenForSubmission:!1,hash:"e29f9949691e86e226d6c7f7aa81134c",slug:"lower-urinary-tract-dysfunction-from-evidence-to-clinical-practice",bookSignature:"Ran Pang",coverURL:"https://cdn.intechopen.com/books/images_new/7957.jpg",editedByType:"Edited by",editors:[{id:"186524",title:"Prof.",name:"Ran",surname:"Pang",slug:"ran-pang",fullName:"Ran Pang"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"684",title:"Endometriosis",subtitle:"Basic Concepts and Current Research Trends",isOpenForSubmission:!1,hash:"1f5625375189846e4fa04200c135afcc",slug:"endometriosis-basic-concepts-and-current-research-trends",bookSignature:"Koel Chaudhury and Baidyanath Chakravarty",coverURL:"https://cdn.intechopen.com/books/images_new/684.jpg",editedByType:"Edited by",editors:[{id:"83747",title:"Prof.",name:"Koel",surname:"Chaudhury",slug:"koel-chaudhury",fullName:"Koel Chaudhury"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"707",title:"Hysterectomy",subtitle:null,isOpenForSubmission:!1,hash:"219d88512350b2e1d01cfd8faf81aa9c",slug:"hysterectomy",bookSignature:"Ayman Al-Hendy and Mohamed Sabry",coverURL:"https://cdn.intechopen.com/books/images_new/707.jpg",editedByType:"Edited by",editors:[{id:"54087",title:"Dr.",name:"Ayman",surname:"Al-Hendy",slug:"ayman-al-hendy",fullName:"Ayman Al-Hendy"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1900",title:"In Vitro Fertilization",subtitle:"Innovative Clinical and Laboratory Aspects",isOpenForSubmission:!1,hash:"212b5ed00828501488c8d7025d84a188",slug:"in-vitro-fertilization-innovative-clinical-and-laboratory-aspects",bookSignature:"Shevach Friedler",coverURL:"https://cdn.intechopen.com/books/images_new/1900.jpg",editedByType:"Edited by",editors:[{id:"111647",title:"Prof.",name:"Shevach",surname:"Friedler",slug:"shevach-friedler",fullName:"Shevach Friedler"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"6278",title:"Pelvic Floor Disorders",subtitle:null,isOpenForSubmission:!1,hash:"e53630ad8f02658c6ca31163f9d68193",slug:"pelvic-floor-disorders",bookSignature:"Raheela M. 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\n\t\t\t
1. Introduction
\n\t\t\t
Land-use management decisions are confronted since ever with the challenge to consider complex interactions of different land-use types - natural ecosystems and man-made systems - and to balance at the same time various needs of different land-users (Dragosits et al., 2006; Kallioras et al., 2006; Letcher & Giupponi, 2005; Niemelä et al., 2005). Changing frame conditions such as Climate Change, changing intensity of land-use, changing impact by deposition, etc. impact eco- or man made systems, lead to a severe disturbance of system specific processes and lower in consequence the system stability and resilience (see e.g. Goetz et al., 2007; Metzger et al., 2006; Callaghan et al., 2004).
Back-coupled on landscape level, the effects of changing frame conditions on individual eco- or man-made systems impact neighbouring systems and might endanger the fulfilment of socially requested functions, goods and services (Fürst et al., 2007a) such aus Carbon sequestration (Schulp et al., 2008), water balance and provision of drinking water (Tehunen et al., 2008). These back-coupling effects must be considered in a holistic land-use management planning approach (Jessel & Jacobs, 2005; Bengtsson et al., 2000).
\n\t\t\t
This becomes even more important with regard to changes in land-use philosophy and intensity such as the increased biofuel crop production and its multi-facetted environmental impact (Demirbas, 2009; Stoeglehner & Narodoslawsky, 2009).
\n\t\t\t
To ensure a sustainable environmental development on the one hand and a sustainable provision of socially requested goods and services on the other, process knowledge must be an integral part of management planning decisions.
\n\t\t\t
A process knowledge oriented land-use management demands:
Furthermore, instruments are demanded which are apt to deal with challenges such as the sectoral fragmentation of information on landscape level, missing data communication standards and which allow for complex knowledge and experience management (Mander et al., 2007; Van Delden et al., 2007; Wiggering et al., 2006).
Last but not least, such tools and instruments must fullfill the criterion of being designed in a user-friendly way to ensure their use in practice (Uran & Jansen, 2003).
\n\t\t\t
The book chapter gives an introduction on process-integration into management decisions, starting with the choice of adequate process-indicators and a condensed overview on process-oriented management support approaches.
\n\t\t\t
Focus is laid on the presentation of the software “Pimp your landscape” (P.Y.L.) and its application areas including some examples. The potential of P.Y.L. to support the integration of processes into land-use management decisions are discussed and remaining development tasks are identified.
\n\t\t
\n\t\t
\n\t\t\t
2. Integration of environmental processes in land-use management decisions
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The landscape is the integrative platform, where interactions and processes meet. Interactions are given between the land-users and decide upon land-use pattern changes. The land-use types interact between themselves and with their environment, with impact on environmental processes. These are pre-adjusted by the (regionally specific) environmental frame conditions, but the latter, such as regional climatic frame conditions or site potentials can be impacted again by land-use pattern changes. Figure 1 proposes a respective conceptual framework for process-oriented land-use management.
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A process-oriented land-use management must consider this network of processes and interactions and is furthermore confronted with the challenge to bring together the three pillars of sustainability (i) the ecological view emphasizing environmental and ecosystem processes. On the other hand, also (ii) the economic view must be kept to optimize land-use management planning and decision making. And (iii) the (regionally specific) societal demands and frame conditions must be considered (Fürst et al., 2007a).
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The DPSIR approach discussed e.g. by Mander et al. (2005) is a suitable and widely spread methodological framework for dealing with environmental management processes in a feedback loop, which controls the interactions within the cycle of Drivers–Pressures–State–Impact–Responses. The DPSIR-approach, demands (i) for a set of suitable indicators and (b) for process-models, which provide information on eco- and man-made system reactions under changing (environmental) frame conditions. Climate change as an example is one of the most important challenges for the future. Its complex impact on land-use management and the potential of single land-use types to contribute in the future to socially requested services and functions on landscape level are still under debate (Harrison et al., 2009; Prato, 2008, Metzger et al., 2006; Hitz & Smith, 2004). For supporting the integration of climate change induced processes into sustainable land-use management decisions, both - indicators and models - must be integrated into intelligent system solutions, which help to come to a common understanding and acceptance of process-based management decisions.
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2.1. Process-indicators
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Suitable process indicators must be apt to describe course, direction and progress of processes in single eco- or man-made systems. Furthermore, they should allow for an upscaling of such processes on landscape level (Fürst et al., 2009; Zirlewagen, 2009;
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Figure 1.
Conceptual framework of process-oriented land-use management: land-use management decisions consider the close connection of interactions and processes on landscape level and are based on indicators, which reflect environmental processes and on decision criteria resulting from the interacting land-users.
\n\t\t\t\t\tHerrick et al. (2006) highlightened the weakness of single indicators such as vegetation composition to conclude on ongoing ecosystem processes and proposed to combine the indicator vegetation composition with other process-indicators such as soil and site stability, hydrologic function and biotic integrity. Fürst et al. (2007b) propose a framework of change-ratio oriented indicators in forest ecosystems, which includes information on the natural frame conditions, man-made changes and temporal development. Nigel et al. (2005) analysed existing sets of criteria and indicators for biodiversity management impact in forests and agricultural land-use and propose a landscape oriented approach how to evaluate changes.
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Concluding from research on appropriate process-indicators leads to the problem that process-indicator-based management planning is not yet realizable in practice, because the necessary holistic aggregation of single indicators or indicator sets from single ecosystems or land-use types with focus on single landscape services is still in progress (Therond et al., 2008).
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2.2. Process-oriented management support tools and systems
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To support the integration of environmental processes into management decisions, several scientific and technological approaches are used. The challenge to integrate manifold indicators and information as output of process-models into process-oriented decisions is picked up by computer-based management and decision support systems (MSS, DSS). They are drawing high attention as a means of improving the quality and transparency of decision making in natural resource management (Rauscher, 1999). Beyond, an increasing number of stakeholders, which are involved in natural resource management and the resulting necessity to consider multiple interests and preferences in the decision-making process led to the use of Multi-Criteria Decision Making (MCDM) techniques in DSS development. Collaborative technologies such as Group Decision Support Systems (GDSS) might help to avoid the consequences of knowledge fragmentation and will extend that support to decision-making processes involving several individuals. Mendoza & Martins (2006) remarked however that a paradigm shift is necessary in existing MCDM approaches to come from methods for problem solving to methods for problem structuring to ensure better support for the user.
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\n\t\t\t\t\tRiolo et al. (2005) e.g. propose a combination of agent-based models and GIS to come to an integration of spatio-temporal processes into management decisions. Castella & Verburg (2007) tested a combination of process- and pattern-oriented models for decisions related to land-use changes. Le et al. (2008) used a multi-agent based model for simulating spatio-temporal processes in a coupled human–landscape system. From a review of existing multi-agent models (MAS), Bousquet & Le Page (2004) came to the conclusion that these mostly interdisciplinary approaches are helpful in complex decision situations.
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However, Malczewski (2004) analysed appropriate systems for supporting the integration of processes and process-knowledge into management decision and compared different tools for GIS-based land-use suitability analysis. His analysis comprised methods such as GIS-based modelling and overlay mapping, multicriteria decision making and artificial intelligence methods (fuzzy logic, neural networks, cellular automatons, etc.). He highlightened, that the major limitation of GIS-based modelling and overlapping is the lack of well defined mechanisms for incorporating decision-makers preferences. Uran & Jansen (2003) found additionally that the lack of user friendliness is the reason, why most of these systems fail to be used in practice. According to Malczewski (2004), the main problem of multicriteria decision making consists in the high variability of methods, which are applied and the fact that the selection of different methods may produce different results. Considering artificial intelligence methods, Malczewski (2004) criticised in general their ‘black box’ style, which makes it difficult for the user to understand how spatial problems are analysed and how the results are produced.
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Concluding from the research and comparison of existing tools and systems, (a) transparency how environmental processes and interactions are handled in the approach and how the results are produces, (b) user friendliness and (c) allowance for user dialog and user interactions seem to be the most important features (see also Diez & McIntosh, 2009).
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3. Pimp your landscape - a process-oriented management support tool
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3.1. Idea and conception
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“Pimp your landscape” (P.Y.L.) was designed to support the understanding of complex interactions between various land-use types on landscape level and to provide a basis to evaluate the impact of user-made land-use pattern changes on most important land-use services. Therefore, the continuous spatial problem “landscape” must have been divided into spatially distinct units, which can interact and communicate with each other and to which different attributes can be assigned.
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The mathematical approach, which has been chosen to reflect complex spatial interactions, was a cellular automaton with Moore-neighbourhood ship. Cellular automata were first introduced by Ulam (1952) and their potential to support the understanding of the origin and role of spatial complexity was highlightened by Tobler (1979). The approach was e.g. used to model urban structures and land-use dynamics (Barredo et al., 2003; White et al., 1996; White & Engelen, 1994, 1993), regional spatial dynamics (White & Engelen, 1997), or the development of strategies for landscape ecology in metropolitan planning (Silva et al., 2008). Nowadays, cellular automata are broadly used to simulate the impact of land-use (pattern) changes and landscape dynamics (e.g. Moreno, et al., 2009; Wickramasuriya et al., 2009; Yang et al., 2008; Holzkämper & Seppelt, 2007; Soares-Filho et al., 2002).
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The starting point in P.Y.L. are land cover datasets, which are taken from Corine Landcover (CLC) 2000 or national level (biotope type / land-use type maps). The smallest unit in the P.Y.L. maps is the cell, which represents an area of 100x100 m² (CLC 2000) or 10x10 m² (only special test sites based on land register maps). A cell can only be attributed with one land-use type. Land-use types with a small share within a cell are assigned to the dominating land-use type. Furthermore, multiple other attributes can be imported as geo-referenced information layer (text or shape files) and can be assigned to the cells, such as geo-pedological information, topographical parameters and climate characteristics. Also, linear elements such as rivers, roads, railways or point-shaped elements of less than 100x100 m² such as power plants can be assigned to a cell. Regarding point-shaped elements, the extent of their spatial impact (e.g. deposition impact gradient) can be defined in the system.
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Either it is possible to assign manually additional attributes to a cell, if digital information is not available. In opposite direction, information from P.Y.L. can be exported as geo-referenced text or shape file to a GIS.
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The core of P.Y.L. is a hierarchical approach to evaluate the impact of land-use pattern changes, which are induced by the user, on land-use services and functions (Fig. 2).
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The evaluation starts by selecting the land-use types (biotope types / ecosystem types), which are of regional relevance and by defining the land-use services and functions of regional interest. The land-use classification standards of CLC 2000 and the land-use services and functions (LUF) set described by Perez-Soba et al. (2008) are available as initial settings. The user can modify these initial settings or adopt completely different settings according to the regional application targets.
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In a next step, indicator sets are identified, which provide information on the impact of the land-use types on land-use services and functions. This step requires several feed-back loops with regional experts: a major problem in the holistic evaluation on landscape level consists (a) in the different scales and dimensions of indicator sets at the different land-use types (Fürst et al., 2009) and (b) in the regional availability of respective knowledge sources. Therefore, a meaningful selection and weighting of the indicators is requested, which respects also regional expert knowledge and experiences to compensate existing knowledge gaps.
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Based on the indicator sets, the impact of each land-use type on each land-use service or function is evaluated on a relative scale from 0 (worst case) to 100 (best case). The introduction of this relative scale enables (a) to compare the impact of different land-use
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Figure 2.
Hierarchical evaluation of the impact of land-use pattern changes.
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types on an individual land-use service or function. (b) The setting of a relative scale as reference supports also a multifunctional evaluation, which faces the challenge to make comparable reactions of different land-use services and functions on land-use pattern changes.
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The resulting (regional) value table represents initial impact values of the land-use types on the services and functions. These must be regionalized to consider (a) the cell specific environmental frame conditions (e.g. height above sea level, mean annual precipitation and temperature, soil type and exposition) and (b) the neighbourhood of different land-use types. This step is supported by rule-sets, which offer the user the possibility to specify a possible increase or decrease of the initial value in dependence from neighbourhood type (homogeneous land-use types different land-use types, edge to edge corner to corner) and in dependence from the (available) environmental attributes.
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Building upon the regionalized evaluation basis, landscape structure indices (landscape metrics) are introduced to adopt the evaluation of “soft” land-use services and functions referring to biodiversity or services related to the aesthetical value of a landscape. The indices help to integrate the heterogeneity of the land- use pattern, the size and connectivity of patches and the form of patches from the holistic landscape view (e.g Uuemaa et al., 2009).
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In addition, the user is offered various options to insert regional planning rules and restrictions. These limit the degree of freedom to which the land-use pattern can be modified.
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The user can specify (a) rules in dependence from the land-use pattern, such as if a land-use type can be converted into another, if a land-use type restricts the conversion of a neighbouring land-use type or if a linear element (street, water body) restricts the conversion of the land-use type at the cell to which this element is assigned.
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Also rules for the spatial development of a land-use type can be defined, such as minimum or maximum thresholds and growth trends, i.e. if the share of a land-use type can increase, decrease or should remain equal.
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(b) Rules depending from environmental frame conditions can be specified, such as if a land-use type is allowed to be converted into another in dependence from pedo-geological, topographical or climatic attributes. Here, the user can choose between the definition of value ranges of the attributes and the definition of upper or lower thresholds.
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(c) Thresholds for the selected land-use services and functions can be defined. According to the evaluation logic, these must adopt a value between 0 and 100.
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Taking the rules into account, the user can start the simulation and can start to modify the land-use pattern. He receives a feed-back on the impact of his changes on the land-use services and functions in real time: the system sums up the value of each cell for each land-use type and divides these sums by the total number of cells, which are displayed in the simulation. A mean value is calculated for each land-use service and the evaluation result is displayed as star diagram.
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The evaluation result is based on the assumption that each land-use type as soon as it is established has its full impact on the land-use services and functions (time point tn). To come to a more realistic evaluation, the possibility to switch between the evaluation results at different time slots of 10, 30, 50 and 100 years is actually integrated into the system (time points tn-m, … tn-p).
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3.2. Application areas and examples
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P.Y.L. allows the user to test the complex and various effects of land-use pattern changes and the establishment of linear and point-shaped infrastructural elements on land-use services and functions by simple mouse click (Fig. 3).
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The user can conduct local changes (cell by cell, freehand shape, establishment of a point-shaped element) or regional changes (changing all cells of a land-use type / changing all cells of a land-use type, which are spatially connected, establishment of linear elements).
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In the philosophy of the system, natural transition processes between land-use types or ecosystems are not considered: the vision of the system is to teach the user the understanding of the effects of his actions on landscape level without additional impact factors, which he cannot influence.
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In land-use management planning, P.Y.L. is adapted and tested for different application areas:
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Figure 3.
Graphical user interface of P.Y.L. with variable options to modify the land-use pattern and to introduce linear / point-shaped elements (icons).
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testing the effects of a regional application of rules and restrictions derived from EU regulations, such as EU Water Framework Directive (2000/60/EC) and Natura 2000 (79/409/EEC and 92/43/EEC) on regionally important land-use services and functions
testing different planning alternatives for the spatial development of urban areas and the establishment of infrastructural facilities, such as highways, railways and roads and deriving the extent of possible compensation measures to keep a politically / socially requested level of land-use services and functions such as live quality, biodiversity, etc.
testing the effects of flooding in the frame of open cast mining area restoration and of participatory elements in landscape planning (recreation areas and areas reserved for natural succession vs. establishment of touristic infrastructure)
testing the effects of climate change on regional risks and potentials and on possible mitigation strategies through changes in the land-use.
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In case (a) - (c), additional effects of changing climatic frame conditions are considered, while responses to climate change are the focal point in case (d).
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Considering (d), the impact of different climate change scenarios is currently tested at the model region “Dresden” (Saxony /Germany) in the project REGKLAM (Development and Testing of an Integrated Regional Climate Change Adaptation Programme for the Model Region of Dresden, www.regklam.de). Regionalized climate change scenarios are combined with soil and topographical data to derive scenario specific risk maps for erosion and drought. These are used as layers in P.Y.L. instead of primary climate, geological and topographical parameters. In a first step and based on a region specific evaluation, it is tested, how the actual land-use pattern increases or decreases the drought and erosion risk. In a next step, planning scenarios for urban growth, spatial development of forestry and agriculture are combined with the risk maps to get (a) information on possible range of responses to regional climate change impact by land-use pattern changes and (b) on areas, where additionally land-use type specific changes in management are demanded.
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\n\t\t\t\t\tFigs. 4-5 show a typical run at the model region Leipzig (Saxony / Germany), where the effects of building a highway are evaluated on regional level (4) and with local focus (5) and where a compensation measure (increase of regional forests from 12 to 30 %, Fig. 6) and finally the possible impact of the construction of a lignite power plant with well described gradient (7) are tested. The star diagram displays the effects of the planning measures for five regionally selected landscape services, the drinking water quality, the aesthetical value of the landscape, climate change sensitivity (based upon regionalized climate change scenarios), regional economy and human health.
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The example reveals also a still existent problem in the evaluation: the impact of linear elements on a region (based on the model of a cellular automaton) is hardly appraisable. Here, the switch between two evaluation perspectives, the regional one (Fig. 4,5,6,7) and the local one (Fig. 5) helps to approximate to the impact of this planning measure. On the other hand, the increase of the forest area seems to overcompensate the highway construction and also the power plant construction. Here, the adjustment of the evaluation result by landscape metrics is still outstanding.
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Figure 4.
Test of the impact of a highway construction on regional level.
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Figure 5.
Switch to the local impact of the highway with focus on a planned motorway junction.
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Figure 6.
Test of a large scale compensation measure by increasing the share of forest land from 12 to ca. 30 %.
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Figure 7.
Testing of the sensitivity of the compensation measure “afforestation” against the additional establishment of a power plant with western deposition gradient.
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4. Discussion and conclusions
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“Pimp your landscape (P.Y.L.)” was developed since 2007 to support process-knowledge integration into land-use management planning decisions on landscape level (Fürst et al., 2008). The integration of process-knowledge is realized by several characteristics of the system:
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the mathematical approach of a cellular automaton enables to simulate by a set of rules dynamic interactions between land-use types and to consider the spatial complexity at landscape level (White et al., 1997).
GIS features of P.Y.L. enable to overlay various land-use pattern scenarios with various environmental parameters, which can also be scenario-driven, such as e.g. climate data (as primary data set) or risk maps (as secondary data set) etc.
The evaluation approach comprises a complex bundling process of indicators and expert knowledge, which is highly sensible for specific regional demands, changing evaluation targets and variable societal demands.
The process of changing the land-use pattern and adding linear of point-shaped elements with their resulting impact on land-use services and functions is strictly driven and defined by the user on the basis of his planning questions and the planning alternatives, he wants to test. Therefore, the criterion transparency is given and as requested by Mendoza & Martins (2006), “decision making” is replaced by support in “problem structuring and testing”.
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Compared to complex spatial decision or management support approaches, P.Y.L. is based on knowledge, which might be derived from modelling, but takes its results not by coupling of models as e.g. done by Le et al. (2008) or Castella et al. (2007). Therefore, also no transition probabilities between different land-use types and historical land-use development can be simulated. This shortcoming in applicability to real world was tolerated with regard to the intention to make better understandable the effects of user-driven land-use pattern changes.
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The requested complex process of knowledge bundling and the identification and selection of indicators and their combination with expert knowledge and experiences must be moderated by science individually for each region and can only build upon results from comparable regions. Furthermore, the use of a relative scale from 0 to 100 to evaluate the impact of land-use changes on land-use services and functions gives no quantitative, but only qualitative information. A resulting risk, which is not specific for P.Y.L. but applies for all knowledge management and decision support systems, is the improper parameterization and use and hereby derived inaccurate decisions (Richardson et al., 2006). However, if the evaluation process is managed well under close participation of regional experts and with detailed documentation of the knowledge sources, the evaluation results in P.Y.L. can experience a high regional acceptance. The easy adaptation of the evaluation base and the rule systems supports also testing how the “system landscape” reacts under variable assumptions on the future value of land-use types for land-use services and functions.
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Finally, a possible problem can occur in the case that P.Y.L. is used at different scale levels in a region, as actually tested in the frame of the REGKLAM project. Moreno et al. (2009) e.g., highlighten the sensitivity of cellular automata to cell size and neighbourhood configuration. Furthermore, problems in the classification logic can appear, when assigning land-use types over different scale levels to the dominating land-use type in a cell. Last but not least, also landscape metrics react sensible on scale level changes (Pascual-Hortal & Saura, 2007; Uuema et al., 2005). Here, approaches how to bridge scale level problems and recommendations for the proper use of the system are actually under development.
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\n\t\n',keywords:null,chapterPDFUrl:"https://cdn.intechopen.com/pdfs/9657.pdf",chapterXML:"https://mts.intechopen.com/source/xml/9657.xml",downloadPdfUrl:"/chapter/pdf-download/9657",previewPdfUrl:"/chapter/pdf-preview/9657",totalDownloads:2717,totalViews:220,totalCrossrefCites:0,totalDimensionsCites:0,totalAltmetricsMentions:0,impactScore:0,impactScorePercentile:19,impactScoreQuartile:1,hasAltmetrics:0,dateSubmitted:null,dateReviewed:null,datePrePublished:null,datePublished:"April 1st 2010",dateFinished:null,readingETA:"0",abstract:null,reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/9657",risUrl:"/chapter/ris/9657",book:{id:"3646",slug:"process-management"},signatures:"Christine Furstm Katrin Pietzsch, Carsten Lorz and Franz Makeschin",authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Integration of environmental processes in land-use management decisions",level:"1"},{id:"sec_2_2",title:"2.1. Process-indicators",level:"2"},{id:"sec_3_2",title:"2.2. Process-oriented management support tools and systems",level:"2"},{id:"sec_5",title:"3. Pimp your landscape - a process-oriented management support tool",level:"1"},{id:"sec_5_2",title:"3.1. Idea and conception",level:"2"},{id:"sec_6_2",title:"3.2. Application areas and examples ",level:"2"},{id:"sec_8",title:"4. Discussion and conclusions",level:"1"}],chapterReferences:[{id:"B1",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBarredo\n\t\t\t\t\t\t\tJ. I.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tKasanko\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMc Cormick\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLavalle\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2003 Modelling dynamic spatial processes: simulation of urban future scenarios through cellular automata. 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R.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHuntley\n\t\t\t\t\t\t\tB.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tIms\n\t\t\t\t\t\t\tR. A.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSitch\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2004 Effects of changes in climate on landscape and regional processes, and feedbacks to the climate system. Ambio, 33(7), 459\n\t\t\t\t\t468\n\t\t\t\t\n\t\t\t'},{id:"B8",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCastella\n\t\t\t\t\t\t\tJ. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tVerburg\n\t\t\t\t\t\t\tP. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2007 Combination of process-oriented and pattern-oriented models of land-use change in a mountain area of Vietnam. Ecological Modelling, 202(3), 410\n\t\t\t\t\t420\n\t\t\t\t\n\t\t\t'},{id:"B9",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCastella\n\t\t\t\t\t\t\tJ. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPheng\n\t\t\t\t\t\t\tKam. 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T.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tStrandman\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008 Sensitivity of managed boreal forests in Finland to climate change, with implications for adaptive management. Philosophical Transactions of the Royal Society B: Biological Sciences, 363(1501), 2341\n\t\t\t\t\t2351\n\t\t\t\t\n\t\t\t'},{id:"B26",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLe\n\t\t\t\t\t\t\tQ. B.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPark\n\t\t\t\t\t\t\tS. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tVlek\n\t\t\t\t\t\t\tP. L. G.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCremers\n\t\t\t\t\t\t\tA. B.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008 Land-Use Dynamic Simulator (LUDAS): A multi-agent system model for simulating spatio-temporal dynamics of coupled human-landscape system. I. Structure and theoretical specification. 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Forest Policy and Economics, 7(6), 877\n\t\t\t\t\t890 .\n\t\t\t'},{id:"B37",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tNigel\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBaldock\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tNasi\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tStolton\n\t\t\t\t\t\t\tS.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2005 Measuring biodiversity and sustainable management in forests and agricultural landscapes. Philosophical Transactions of the Royal Society B: Biological Sciences, 360 (1454), 457\n\t\t\t\t\t470\n\t\t\t\t\n\t\t\t'},{id:"B38",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tOlesen\n\t\t\t\t\t\t\tJ. E.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBindi\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2002 Consequences of climate change for European agricultural productivity, land-use and policy European. 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Environment and Planning A, 25, 1175\n\t\t\t\t\t1199\n\t\t\t\t\n\t\t\t'},{id:"B60",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWhite\n\t\t\t\t\t\t\tR.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tEngelen\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tUljee\n\t\t\t\t\t\t\tI.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1997 The use of constrained celluar automata for high-resolution modelling of urban land-use dynamics, Environment and Planning B: Planning and Design 24, 323\n\t\t\t\t\t343\n\t\t\t\t\n\t\t\t'},{id:"B61",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWickramasuriya\n\t\t\t\t\t\t\tR. C.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tBregt\n\t\t\t\t\t\t\tA. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tvan Delden\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHagen-Zanker\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2009 The dynamics of shifting cultivation captured in an extended Constrained Cellular Automata land use model. Ecological Modelling, 220(18), 2302\n\t\t\t\t\t2309\n\t\t\t\t\n\t\t\t'},{id:"B62",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWiggering\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDalchow\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tGlemnitz\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHelming\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tMüller\n\t\t\t\t\t\t\tK.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchulz\n\t\t\t\t\t\t\tA.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tStachow\n\t\t\t\t\t\t\tU.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZander\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2006 Indicators for multifunctional land use- Linking socio-economic requirements with landscape potentials. Ecological Indicators, 6, 238\n\t\t\t\t\t249\n\t\t\t\t\n\t\t\t'},{id:"B63",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWrbka\n\t\t\t\t\t\t\tT.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tErb\n\t\t\t\t\t\t\tK. H.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSchulz\n\t\t\t\t\t\t\tN. B.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPeterseil\n\t\t\t\t\t\t\tJ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHahn\n\t\t\t\t\t\t\tC.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHaberl\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2004 Linking pattern and process in cultural landscapes. An empirical study based on spatially explicit indicators. Land Use Policy, 21(3), 289\n\t\t\t\t\t306\n\t\t\t\t\n\t\t\t'},{id:"B64",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tYang\n\t\t\t\t\t\t\tQ.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLi\n\t\t\t\t\t\t\tX.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tShi\n\t\t\t\t\t\t\tX.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2008 Cellular automata for simulating land use changes based on support vector machines. Computers and Geosciences, 34(6), 592\n\t\t\t\t\t602\n\t\t\t\t\n\t\t\t'},{id:"B65",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZirlewagen\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2009 Regionalisierung der bodenchemischen Drift in der Dübener Heide im Zeitraum 1995-2006. Waldökologie, Landschaftsforschung und Naturschutz, 8, 21\n\t\t\t\t\t30\n\t\t\t\t\n\t\t\t'},{id:"B66",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZirlewagen\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tvon\n\t\t\t\t\t\t\tWilpert. K.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2009 Raum-Zeitmuster von Stoffflüssen im Boden: Verbindung von Sickerwasserchemie und Bodenfestphase, Waldökologie, Landschaftsforschung und Naturschutz, 8, 31\n\t\t\t\t\t40 .\n\t\t\t'},{id:"B67",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tZirlewagen\n\t\t\t\t\t\t\tD.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tRaben\n\t\t\t\t\t\t\tG.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWeise\n\t\t\t\t\t\t\tM.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2007 Zoning of forest health conditions based on a set of soil, topographic and vegetation parameters. Forest Ecology & Management, 248(1-2), 43\n\t\t\t\t\t55 .\n\t\t\t'}],footnotes:[],contributors:[{corresp:"yes",contributorFullName:"Christine Fürst",address:null,affiliation:'
Technische Universität Dresden (Dresden University of Technology) Institute for Soil Sciences and Site Ecology
Technische Universität Dresden (Dresden University of Technology) Institute for Soil Sciences and Site Ecology
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1. Introduction
Members of the class Actinobacteria produce an impressive range of bioactive metabolites that are of commercial importance and many more that have the potential for future exploitation. This includes biosurfactants which are synthesised by many actinobacterial species. Microbial biosurfactants are gaining increased attention in the biotechnology industries as they are multifunctional, enabling diverse applications. Biosurfactants can also claim strong green credentials as not only are they biorenewable with the possibility of production on various substrates including wastes, but they may also be applied to environmental remediation [1]. Further, biosurfactants are generally considered superior to their chemically synthesized counterparts. Amongst the most common biosurfactant producers are members of the mycolic acid-containing (mycolate) genus Rhodococcus which have received considerable attention. However, other related mycolate genera including Corynebacterium, Dietzia, Gordonia and Tsukamurella also possess biosurfactant-producing strains but have not been explored to the same extent. Additionally, there are several other mycolate genera that have received little or no investigation in this respect that may produce novel biosurfactant compounds.
Membership of the mycolic acid-containing actinobacterial (MACA) group has expanded considerably over the past 20 years with revisions to the classification of existing species and the publication of copious new mycolate species and genera [2]. This substantial and metabolically diverse group therefore warrants further attention in the search for valuable biosurfactants. This chapter provides an overview of the current knowledge on biosurfactants produced by members of this group and describes approaches to the recovery, screening and biosurfactant-producing strains from the environment and their growth requirements. Methodologies applied to screen for biosurfactant production and for extraction, purification, and structural elucidation of biosurfactant compounds are also described. Current and potential future applications of biosurfactants derived from MACA are examined with particular focus on potential biomedical and environmental possibilities.
1.1 Biosurfactant properties
Microbial biosurfactants are amphipathic compounds, with both hydrophilic (polar) and hydrophobic (non-polar) moieties. The hydrophobic portion has saturated, unsaturated, or hydroxylated long-chain fatty acids and the hydrophilic portion can contain amino acids, carbohydrate, carboxyl acid, peptides, phosphate, or alcohol [3]. Biosurfactants may be categorised according to molecular weight (low or high), ionic charge (anionic, cationic, neutral, or non-ionic) or according to chemical composition and structure. The main classes of biosurfactants include fatty acids, glycolipids, lipopeptides, lipoproteins, neutral lipids, phospholipids, and polymeric biosurfactants. Their amphipathic nature enables biosurfactants to partition at water-air, oil-air, or oil-water interfaces thereby reducing surface and/or interfacial tension. They exhibit many other useful properties including de-/emulsification, dispersion, foaming, lubrication, softening, stabilisation, viscosity reduction and wetting [4].
Biosurfactants may be located intracellularly, on the cell surface (cell-bound) or excreted extracellularly (free) [5] and are produced during growth on both hydrophilic and hydrophobic substrates, to reduce surface or interfacial properties of the microbial cell or the surrounding environment. Biosynthesis of these compounds is required for gliding, motility, swarming, and biofilm formation. Biosurfactants also mediate between cells and hydrophobic compounds, enabling enhanced solubilisation and uptake across the cell membrane for utilisation as a substrate for growth and energy (Figure 1).
Figure 1.
Emulsification of hydrocarbons by microbial biosurfactants to enhance bioavailability.
Many microbially derived biosurfactants are already used in diverse industries including agriculture, bioremediation, cosmetics, food, healthcare and medicine, and the petrochemical industry (Figure 2). In addition to being multifunctional, biosurfactants have several advantages over chemically synthesised surfactants. They are less/non-toxic and biodegradable, have higher surface activity and lower critical micelle concentrations (CMC), greater biocompatibility and selectivity, they function over wide pH, salinity, and temperature ranges, and can be produced using renewable and waste substrates [6]. These unique eco-friendly features make biosurfactants particularly attractive options as industries focus on longer-term sustainability and working towards a circular economy.
Figure 2.
Various sectors of application for microbial biosurfactants.
1.2 Mycolic acid-containing actinobacteria
The MACA form a phylogenetically coherent group that resides in the order Corynebacteriales based on 16S rRNA gene sequence analysis. The members are Gram-positive with high guanine-plus-cytosine (G + C) content in their genomic DNA. They currently comprise more than 400 species classified in 15 genera, namely Corynebacterium, Dietzia, Gordonia, Hoyosella, Lawsonella, Millisia, Mycobacterium, Nocardia, Rhodococcus, Segniliparus, Skermania, Smarigdococcus, Tomitella, Tsukamurella and Williamsia [2]. The almost universal production of mycolic acids by members of this group is a synapomorphic trait that is unique to this phylogenetic lineage [7]. However, several members of this order appear to have lost the ability to produce mycolic acids over the course of evolution, including several species of the genus Corynebacterium and Hoyosella. It was recently proposed that the single species belonging to the genus Turicella, also characterised by the absence of mycolic acids, be reclassified in the genus Corynebacterium [8].
Mycolic acids, which are high molecular weight 3-hydroxy fatty acids with a long alkyl branch in the 2-position, represent the major lipid constituents of the cell envelope of these organisms. They show structural variations from relatively simple mixtures of saturated and unsaturated compounds in corynebacteria to highly complex mixtures in mycobacteria. Mycolic acids also vary in the number of carbons on the 2-alkyl-branch from C22–C38 in corynebacteria to C60–C90 in mycobacteria [9]. Mycolic acids play an essential role in the architecture and functions of the cell envelope, where attached to the cell wall arabinogalactan they help to form a barrier that contributes to impermeability and resilience and conveys hydrophobicity to the cell surface. Trehalose mycolates, also termed cord factors, play an important role in pathogenicity in mycobacterial species that cause infection [9]. The presence and carbon chain length of mycolic acids can be used as taxonomic markers for the identification and classification of actinobacteria to the order Corynebacteriales [2].
Members of order Corynebacteriales can usually be distinguished from one another and from corresponding taxa in the phylum Actinobacteria based on 16S rRNA phylogeny supported by phenotypic (cell wall chemistry and morphology) features. Cell morphology amongst the MACA varies from simple rods and cocci to branched filaments that fragment to pleomorphic forms (Table 1). Members of the species Skermania piniformis are micromorphologically unique in this group as they form pine tree-like acute-angle branched filaments [10]. Colonies growing on agar plates are normally visible within several days of inoculation (Figure 3) although slow-growing mycobacteria take considerably longer. Species vary widely in colony appearance and are often colourful however it is usually not possible to unambiguously assign strains to a genus based on this feature alone.
Genus
Micro-morphology
Acid-fastness
Aerial hyphae
Visible colonies (days)
Strictly aerobic
Corynebacterium
Pleomorphic rods, often club-shaped in palisade or angular arrangements
Some weakly acid-fast
Absent
1–2
No
Dietzia
Short rods and cocci
No
Absent
1–3
Yes
Gordonia
Rods, cocci and/or moderately branching hyphae
Partially acid-alcohol fast
Absent
1–3
Yes
Hoyosella
Cocci occur singly, in pairs, tetrads or in groups
Slightly acid–alcohol-fast
Absent
2
Yes
Lawsonella
Pleomorphic bacilli and cocci
Partially acid-fast
Absent
5–7
No
Millisia
Short rods
Acid-alcohol fast
Absent
1–3
Yes
Mycobacterium
Rods, occasionally branched filaments that fragment to rods and cocci
Strongly acid-fast
Rare
2–40
Yes
Nocardia
Mycelia that fragment into rods and cocci
Partially acid-fast
Present
1–5
Yes
Rhodococcus
Rods to extensive substrate mycelia that fragment to irregular rods and cocci
Partially acid-fast
Absent
1–3
Yes
Segniliparus
Rods
Acid-alcohol fast
Absent
3–4
Yes
Skermania
Acute angled branched mycelia
No
Only visible under the microscope
10–21
No
Smaragdicoccus
Coccoid
ND
Absent
7–14
Yes
Tomitella
Irregular rods
ND
Absent
ND
Yes
Tsukamurella
Single rods or in pairs or masses, sometimes rudimentary filaments and coccobacillary forms
Partially alcohol-acid fast
Absent
1–3
Yes
Williamsia
Thin rods or cocci in pairs or clusters
ND
Present
1–4
Yes
Table 1.
General phenotypic features of mycolate genera classified in the order Corynebacteriales.
The appearance of (a) Gordonia amarae, (b) Rhodococcus erythropolis and (c) Tsukamurella spumae on glucose yeast-extract agar after 7 days incubation at 30°C.
Chemotaxonomy is the study of the distribution of various cell wall components to classify and identify strains and is particularly useful to differentiate between the various mycolic acid-containing genera. Cell wall markers typically used to differentiate between MACA genera are summarised in Table 2. Some of the methods used to analyse these chemotaxonomic markers provide quantitative or semi-quantitative data, as in the case of fatty acids, whereas other techniques provide only qualitative data as in the case of muramic acid type and phospholipid pattern.
Reliable identification of MACA strains to species level depends upon phylogenetic analysis of the gene encoding 16S rRNA and DNA:DNA homology determination provides definitive delineation of species with 70% homology and above signifying membership of same species [11]. Increasingly, whole-genome sequencing (WGS) is becoming a standard technique and comparative genomic analysis is providing useful insights to the relatedness and divergence of MACA species [11]. Protein sequences from Corynebacteriales genomes have revealed many conserved signature indels (CSIs) conserved signature proteins (CSPs) that are specific for members of this order [12].
2. Biosurfactants produced by MACA
In addition to Rhodococcus, diverse members of the order Corynebacteriales have been reported to synthesise extra-cellular and cell-bound biosurfactants, including members of the genera Corynebacterium, Dietzia, Gordonia, Mycobacterium, Nocardia, and Tsukamurella. Species belonging to the genus Rhodococcus have been most extensively investigated and are known to produce different chemical types, including a variety of glycolipids. However, an interesting array of biosurfactant structures are synthesized by MACA including lipopeptides, oligosaccharide lipids, polymeric glycolipids, terpenoid glycosides, trehalose corynemycolates, trehalose mycolates and dimycolates, and trehalose lipid (THL) esters [13]. Example structures of the different types of biosurfactants produced by MACA are shown in Figure 4. The chemical structure of trehalose-containing glycolipids have perhaps been studied in most detail. Several structural types have been reported including mono-, di- and tri-corynemycolates which have been characterised for species such as Rhodococcus erythropolis, Rhodococcus ruber and Rhodococcus wratislaviensis [14] and trehalose di-nocardiomycolates which have been characterised for Rhodococcus opacus [13]. The mycobacterial trehalose mycolates or di-mycolates (cord factors) are also thoroughly investigated given their role as modulators of mycobacterial pathogenesis and host immune response.
Figure 4.
Types and key structural features of various biosurfactants produced by MACA. (Adapted from [13]).
3. Habitats, recovery, and growth requirements of MACA
MACA are widely distributed in the environment including natural habitats such as mangroves, soil, freshwater, and deep ocean sediments as well as man-made sites such as activated sludge foams, biofilters, industrial wastewater and indoor building materials. Although predominantly saprophytic, many species are opportunistic pathogens forming parasitic associations with plants and animals, including humans, notably immunocompromised individuals. Several members of the genus Mycobacterium cause a plethora of diseases most notably tuberculosis caused by Mycobacterium bovis and Mycobacterium tuberculosis.
MACA capable of producing various biosurfactants have been isolated from environments (Table 3) including oil-contaminated soils [24, 25], water from oil wells [26], wastewater from the rubber industry [21], activated sludge, and effluent and sediment from pesticide manufacturing facilities [23]. The ability of MACA to produce biosurfactants in these habitats appears to be driven by the environmental conditions to which they are exposed whereby the biosurfactants act as mediators for the biodegradation of hydrophobic carbon substrates. Genes involved in biosynthesis of rhamnolipids by Dietzia maris for example have been shown to be upregulated in the presence of hydrophobic substrates including n-hexadecane, n-tetradecane and pristane [15]. However, the true distribution of biosurfactant-producing MACA in the environment may not solely depend on the presence of hydrophobic substrates.
Various environmental sources of biosurfactant-producing MACA.
Isolation of biosurfactant producers largely relies on selective isolation strategies, utilising hydrophobic compounds as sole carbon sources for energy and growth. Typically, strains are isolated and cultivated using mineral salt medium containing essential trace elements supplemented with a hydrocarbon substrate such as crude oil, diesel, n-alkanes, n-hexadecane, paraffin, polyaromatic hydrocarbons (PAHs), or vegetable oils such as olive oil and rapeseed oil, as the sole carbon source. These may be incorporated into the liquid or solid medium, spread across the agar surface or soaked onto a filter in the lid of petri dishes. Besides the selectivity of the culture medium, pre-enrichment techniques utilising hydrophobic compounds as the sole carbon source, can be used [27]. The principle of enrichment is to provide growth conditions that are favourable for the organisms of interest but not for competing organisms. This selective advantage allows target populations to expand through a series of passages, maximising the chances of successful recovery at the isolation stage. Incorporating antibiotics into the isolation media may provide a useful additional selective pressure to eliminate or reduce unwanted fungi and bacteria.
The ability of an organism to grow on hydrophobic compounds is a good indicator of biosurfactant production but is not a guarantee. It is therefore important that isolates of interest are tested in pure culture for biosurfactant production using further screening assays. It is also possible that biosurfactant-producing organisms may be present in an environment but not enriched by in the conditions provided or indeed producers may be recovered from the environment but not synthesize biosurfactants under the culture conditions imposed. Mining genomes for cryptic biosurfactant biosynthesis pathways, and metagenomic screening of DNA from environmental samples promise an alternative approach to biosurfactant discovery that may circumvent some of the issues associated with culture-dependent strategies [28].
4. Detection and characterisation of biosurfactants
4.1 Biosurfactant screening methods
A variety of methods, both qualitative and quantitative, have been applied to screen microbial cultures and cell-free media for total (intracellular, surface-bound, and freely released) and freely released biosurfactants, respectively. As biosurfactants are structurally diverse, complex molecules, most of these methods are indirect, reliant on physico-chemical properties such as emulsification, surface activity or hydrophobicity. Commonly reported screening methods used to detect biosurfactant production amongst MACA strains are listed in Table 4. Besides the bacterial adhesion to hydrocarbons (BATH) assay [37] other tests based on cell surface hydrophobicity include salt aggregation [38] and hydrocarbon overlay [39] assays. The atomized oil assay [40] may be used to directly screen colonies growing on primary isolation plates and is therefore useful as an initial screen for novel-producing strains recovered from the environment. The microplate assay [41] which relies on the wetting properties of biosurfactants and the penetration assay [42], which relies on the reduction of interfacial tension are also considered useful for screening large numbers of strains. Recently, a rapid, high throughput assay that utilises Victoria pure blue BO dye, and is based on surface-active properties, has been developed for quantitative screening, but has not yet been applied to MACA [43].
Examples of screening methods used to detect biosurfactant production by MACA.
These assays are simpler and more rapid than chemical analytical procedures, and most enable larger-scale screening for biosurfactant production. However, perhaps owing to the general and indirect nature of these assays and various limitations associated with some, test results between assays are not always congruent and no one assay is considered definitive for biosurfactant production. It is thus advisable to use several methods in combination, adopting simple methods to undertake preliminary screening of large strains collections prior to further investigation of those found to be most promising. The development of high-throughput screening, metabolic profiling technologies, and whole-genome analysis promise a more thorough investigation of potential biosurfactant producing strain in the future [28].
4.2 Extraction and structural analysis of biosurfactants
Crude biosurfactant extracts may be obtained from cell cultures (cell-associated and free surfactants) or cell-free broth (free surfactant only) by acidification and solidification followed by solvent extraction of the precipitate. In the case of MACA commonly used solvents include MTBE, dichloromethane, or varying ratios of chloroform–methanol or MTBE–chloroform [44]. Various analytical techniques are used in combination to detect, quantify, and characterise biosurfactants. Thin layer chromatography (TLC) is a straightforward method to separate biosurfactant fractions present in crude extracts. Samples are spotted at the base of a silica plate before development in a solvent system, then air-dried and sprayed with a particular reagent to detect certain chemical groups based on spot colour and/or Rf values. Orcinol, for example, allows detection and differentiation of glycolipids and can distinguish mono-rhamnolipid (MRL) and DRL congeners [45]. However, TLC provides little further detail on congener structure, and it is not generally considered suitable for quantitative analysis although densiometry has been used for this purpose [46]. Biosurfactants may be further separated by silica gel column chromatography.
High-performance liquid chromatography-mass spectrometry (HPLC-MS) allows more precise and accurate characterisation and quantitation of biosurfactant compounds. Isocratic HPLC-UV has been reported for structural and yield determination of THLs produced by R. erythropolis strain MTCC 2794 from semi-purified extractions of whole-cell broth [47]. Nuclear magnetic resonance spectroscopy (NMR) is considered the gold standard method to characterise the chemical structure of novel biosurfactants. This has been used in combination with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF/MS) to elucidate the structure of two novel extracellular THLs TL A and TL B from Tsukamurella spp. [18].
A combination of Fourier transform infrared spectroscopy (FTIR), NMR, and liquid chromatography-mass spectrometry (LC-MS) enabled structural characterisation of a novel cyclic lipopeptide, Coryxin, produced by Corynebacterium xerosis NS5 [48]. Multiple-Stage Linear Ion-Trap Mass Spectrometry with Electrospray Ionization has been used to determine the structure of trehalose monomycolate (TMM) and trehalose dimycolate (TDM) in the cell wall of Rhodococcus equi and R. opacus [49]. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been utilised successfully for the purification and characterisation of sophorolipids and rhamnolipids in Pseudomonas aeruginosa [50] and could be applied to similar compounds produced by mycolate species. Gas chromatography-mass spectrometry (GC-MS) is used to characterisation of the fatty acid and mycolic acid components and for the carbohydrate portion of THLs.
5. Potential applications of biosurfactants from MACA
Biosurfactants produced by rhodococci and related MACA have been investigated primarily for their potential application in oil remediation but are otherwise under-studied and under-exploited. However, research studies reveal various potential applications for these molecules, including in environmental and medical fields as summarised in Figure 5.
Figure 5.
Promising medical and environmental applications for biosurfactants produced by MACA.
5.1 Biomedical applications
Biosurfactants produced by microorganisms are reported to have various potential biomedical and pharmaceutical applications which have been reviewed widely [1, 51, 52]. This stems from an array of biological properties including anti-adhesion and antibiofilm, anti-inflammatory, antimicrobial (anti-bacterial, anti-fungal and anti-viral), antioxidant, anti-tumour, and wound healing activities. Other potential applications include adjuvants for antigens in vaccines, pulmonary surfactants, drug delivery systems, enhanced vehicles for gene therapy and in dermatological care. Biosurfactants also have several applications in therapeutic dentistry [53]. Daptomycin, a cyclic lipopeptide produced by the actinobacterium Streptomyces filamentosus, is used as an antibiotic to treat serious blood and skin infections caused by Gram-positive pathogens [54] and there are other examples of actinobacteria that produce surfactants with potential biomedical applications, such as Nocardiopsis strains [55]. Only limited investigation has focused on the biomedical potential of biosurfactants from MACA, except for TDM or cord factors synthesised by intracellular pathogens of the genera Mycobacterium. Nevertheless, as shown in Table 5, studies over the past two decades reveal that various biosurfactants produced by members of the genera Corynebacterium, Nocardia, Rhodococcus, and Tsukamurella demonstrate a range of promising properties.
Strain (origin)
Biosurfactant
Biomedical properties
Reference
C. xerosis NS5 (human axilla)
Purified Coryxin (lipopeptide)
Antibacterial activity, biofilm inhibition and disruption of pre-formed biofilms of Gram-positive S. aureus and Streptococcus mutans and Gram-negative E. coli and P. aeruginosa strains
Anti-tumour activity: cytotoxic effects on human tumour cell lines BV-173 and SKW-3, and to a lesser extent, HL-60. Mediated cell death by the induction of partial apoptotic DNA laddering
N. vaccinii IMB B7405 (K-8) (oil-contaminated soil)
Complex of amino lipids; neutral lipids (mycolic and n-alkanic acids); trehalose di-acelates and di-mycolates (surfactant solution and supernatant)
Anti-adhesive activity against Gram-negative bacteria E. coli, Proteus vulgaris, P. aeruginosa and Enterobacter cloaceae and the yeast Candida albicans on silicon urogenital catheters. Anti-adhesive activity against fungus C. albicans and bacterium E. coli on treated acrylic dental material and against Gram-positive Bacillus subtilis and micromycete Aspergillis niger when coated on various abiotic substrates
In vitro induction of human promyelocytic leukaemia (HL60) cell line differentiation into monocytes and inhibition of protein kinase C
R. erythropolis IMВ Ac-5017 (EK-1) (oil-contaminated soil)
Complex of trehalose mono- and di-mycolates; neutral lipids (cetyl alcohol, palmitic acid, methyl ether of n-pentadecanoic acid, mycolic acids); phospholipids (phosphatidylglycerol, phosphatidylethanol-amine) (surfactant solution and supernatant)
Antibacterial activity against Gram-positive bacteria B. subtilis and S. aureus and Gram-negative E. coli and Pseudomonas sp., and anti-fungal activity against C. albicans, C. utilis and C. tropicalis
Anti-adhesive activity against Gram-negative bacteria and fungus C. albicans on silicon urogenital catheters. Anti-adhesive activity against B. subtilis on various abiotic substrates, against C. albicans and E. coli on acrylic dental material and S. aureus and P. aeruginosa on plastic and steel
R. fascians BD8 (Arctic soil polluted with hydrocarbons
THL
Antibacterial activity against Vibrio harveyi and P. vulgaris, and partial inhibition of other Gram-positive and negative bacteria and fungus C. albicans. Anti-adhesion properties on polystyrene against various Gram-positive and negative strains and fungal strains of C. albicans. Biofilm inhibition on glass, polystyrene, and silicone urethral catheters against Gram-positive Enterococcus hirae and E. faecalis, Gram-negative E. coli, and fungus C. albicans
R. ruber IEGM 231 (spring water, oil-extracting enterprise)
Crude trehalolipids
Anti-adhesive activity against exponentially growing Gram-positive bacteria Arthrobacter simplex, B. subtilis, Brevibacterium linens, Corynebacterium glutamicum, and Micrococcus luteus and against Gram-negative bacteria E. coli and P. fluorescens on polystyrene.
In vitro induction of Th1-polarizing factors IL-12 and IL-18 by human mononuclear cells and monocytes and reactive oxygen species (ROS) by peripheral blood leukocytes
In vivo suppression of bactericidal activity and proinflammatory cytokine IL-1β of mouse peritoneal macrophages, antibody production by splenocytes and stimulates the production of IL-10
Biomedical research on biosurfactants produced by MACA.
The amphipathic nature of biosurfactants makes them suitable for anti-adhesion and anti-biofilm applications such as the development of anti-adhesive coatings for intra-urinary devices that are prone to the formation of intractable biofilms, to prevent or delay the onset of biofilm growth by pathogens such as Escherichia coli and Proteus mirabilis. C. xerosis strain NS5, Nocardia vaccinii K-8 and various Rhodococcus strains demonstrate anti-adhesion, biofilm inhibition and/or biofilm disruption effects against various clinically significant pathogens (Table 5). Some also exhibit antimicrobial properties although in the case of R. ruber strain IEGM 231 the trehalolipids had no effect on cell viability despite preventing adhesion of various bacteria to polystyrene [63]. Oligosaccharides produced by Tsukamurella tyrosinosolvens (DSM 44370) showed some activity against Gram-positive bacteria, although the pathogenic strain Staphylococcus aureus was not affected. Rhodococcus strain I2R shows anti-viral activity against herpes simplex virus 1 (HSV-1) and human coronavirus HcoV-OC43 [62].
Nocardia farcinica BN26 produces a THL with anti-cancer effects, showing cytotoxicity against human tumour and promyelocytic leukaemia (HL60) cell lines [57]. Rhodococcus erythropolis SD-74 and Rhodococcus sp. TB-43 also cause the induction of HL60 cells [59, 60]. R. ruber has been studied in some detailed and reported to show immunomodulatory effects, including both in vitro induction of Th1-polarizing factors IL-12 and IL-18 by human mononuclear cells and monocytes and in vivo induction of IL-1β by mouse peritoneal macrophages [64, 65, 68, 69]. Two succinoyl trehalose lipids, STL-1 and STL-3, produced by R. erythropolis SD-74 inhibit growth and induce cell differentiation into monocytes instead of cell proliferation when tested on the HL60 cell line.
Glycolipid bearing mycolic acids, such as trehalose dimycolate (TDM) have attracted extensive investigation as they play a central role in pathogenesis during infection by intracellular pathogens such as M. tuberculosis and R. equi. TDM’s have been researched as a possible tuberculosis vaccine and as an adjuvant. In addition, modification of mycobacterial TDM has been shown to reduce virulence and suppress the host immune response [9]. Interestingly, TDM also possesses biological activities that point towards medical and pharmaceutical applications, such as antitumor activity and immunomodulating functions. Despite this, the potential for TDM is perhaps limited by relatively high toxicity and the pathogenic nature of the species that produce them.
Although biologics including surfactants are generally regarded as less toxic than synthesized pharmaceuticals not much work has focussed on this with respect to MACA surfactants. However, a THL from R. erythropolis strain 51T7 has been reported to be suitable for use in cosmetic preparations as it was less irritating than SDS when tested on mouse fibroblast and human keratinocyte lines [70]. Further investigation into the potential biomedical and pharmaceutical applications of biosurfactants produced by members of the MACA, including toxicity testing, is certainly warranted. The high costs and technical challenges associated with production and downstream extraction of biosurfactants may not be a barrier to their commercial application in biomedical fields given that smaller-scale productions would likely be required.
5.2 Environmental applications
Biosurfactants have a range of promising, and increasingly important, applications in the environmental, industrial, and agricultural sectors (Table 6). These include bioremediation of both organic pollutants (especially hydrocarbons) and metals, microbial enhanced oil recovery (MEOR), cleaning and maintenance of tanks and pipelines in the petroleum industry, wastewater treatment, and agricultural applications such as promotion of plant growth/health and inhibition of phytopathogenic fungi [1, 78]. MACA-derived surfactants have been investigated in some of these contexts, although the focus is on well-known species such as R. ruber and R. erythropolis. Members of Gordonia, Corynebacterium, Nocardia and Dietzia have also been investigated but there is likely to be much unexplored potential within the group [79]. This is supported by the promising results obtained with rhamnolipids produced by other bacteria, most notably P. aeruginosa, and their commercialisation [80]. It is not unreasonable to expect that rhamnolipids produced by MACA may also exhibit such properties. Indeed, the search for non-pathogenic producers is important for further development of biosurfactant production at industrial scale [81].
Application
Examples of MACAs
Reference/s
Bioremediation: enhanced hydrocarbon solubility and degradation
D. maris As-13-3 D. maris WR3 G. amicalis HS-11 Gordonia cholesterolivorans AMP 10 N. otitidiscaviarum R. erythropolis 3C-9 R. pyridinivorans NT2
Various potential environmental applications of biosurfactants produced by MACA.
Pollution of soils with organic and inorganic chemical compounds is a major environmental issue. Biosurfactants are used to improve the solubility of hydrocarbon organic compounds, either to make them available for subsequent biodegradation or to facilitate removal by soil washing. A remediation agent called JE1058BS containing biosurfactant from Gordonia sp. strain JE-1058 was evaluated as an oil spill dispersant using the baffled flask test recommended by the US Environmental Protection Agency and performed better than commercially available dispersants. It also enhanced the bioremediation of crude oil by indigenous marine bacteria and significantly improved removal of crude oil from contaminated sea sand by washing compared with the use of seawater alone [73]. Various Dietzia, Gordonia and Rhodococcus strains have been shown to degrade hydrocarbon compounds and many studies show that the production of surface-active compounds makes an important contribution. In a recent study, G. amicalis HS-11 was able to remove 92.85% of the diesel oil provided as the sole carbon source after 16 days of incubation, with a corresponding reduction in surface tension due to the production of extracellular surfactants. Microscopy suggested that these surfactants play a role in the emulsification and uptake of the hydrocarbons. Plant-based bioassays also showed that toxicity of the diesel oil decreased. This illustrates the potential of this strain and perhaps other gordoniae for use in the bioremediation of contaminated environments, or industrial wastewaters [82].
The properties and actions of biosurfactants make them particularly relevant to the petroleum industry. MEOR is perhaps the most well-known application in this area. Biosurfactants, or biosurfactant-producing microorganisms, are used to extract some of the oil remaining in reservoirs after primary and secondary processing has been carried out. Mechanisms include reduction of capillary forces holding the oil in porous rock, stabilisation of desorbed oil in water and increased viscosity of oil for easier removal [83]. Dietzia sp. ZQ-4, a hydrocarbon-degrading, surfactant-producing MACA isolated from an oil reservoir, demonstrated potential for use in ex situ oil recovery. Fermentation broth significantly increased oil displacement efficiency by 18.82% in rock cores and performed well within the range reported for other strains. However, injection of the strain itself was not so successful, and field trials testing nutrient injection did not always result in an increase in the population of Dietzia sp. ZQ-4, indicating that an in-situ approach may not be viable although it may be possible to optimise this strategy further [72]. Biosurfactants produced by various rhodococci strains recovered from oil-polluted soils have been shown to be effective at recovering trapped oil from oil-saturated sand packs. Glycolipids produced by strain ST-5 recovered up to 86% [84] and a mix of glycolipids and extracellular lipids produced by strain TA6 up to 86% [24] using the sand pack column method. Studies on biosurfactant produced by R. ruber IEGM 231 showed that 2.5 times greater washing activity could be achieved than with synthetic surfactant Tween-60 in soil columns spiked with polyaromatic carbons (PAHs) and alkanes. The biosurfactant maintained activity at a high (5% w/w) contamination level and consistently removed 0.3–0.5 g PAHs per kg dry soil in a single run of washing [71].
Biosurfactants may also be used to de-emulsify water–oil emulsions that form during oil production in the oilfields, as well as during transportation, and processing and offer a more ecologically friendly solution than chemically synthesized de-emulsifiers. A lipopeptide bio-demulsifier produced by Dietzia sp. strain S-JS-1 grown on waste frying oil achieved 88.3% of oil separation ratio in water/oil emulsion and 76.4% of water separation ratio in oil/water emulsion [75].
Biosurfactants have been shown to reduce phytotoxicity of heavy metals, and pre-treatment of seeds could allow plants to be grown successfully in contaminated soil, facilitating phytoremediation of the environment. Crude biosurfactant from R. ruber IEGM 231 mitigated the toxic effects of high concentrations of molybdenum on oat, white mustard, and vetch seeds. Germination increased up to 4.5 times and shoot and/or root length up to 2.5 times when seeds were pre-treated with a biosurfactant emulsion and grown under conditions of molybdenum contamination [85]. Similar results have been recorded for other heavy metals such as copper [86].
The use of biosurfactants in environmental and industrial applications is limited by the current high costs of production, and the large amounts of biosurfactant required. However, using waste and/or renewable substrates would be cheaper, and a highly purified product is not essential so costs of downstream processing can also be reduced. In addition, different approaches such as selective stimulation of biosurfactant producers in situ, and inoculation of biosurfactant-producing cultures, are being explored [87]. This could potentially overcome some of the challenges associated with accessing the cell-bound biosurfactants produced by MACA such as Rhodococcus spp.
5.3 Challenges to commercialisation
Currently, commercial production of biosurfactants is not economically competitive with chemical surfactant production as there are various challenges to overcome. Bioprocesses presently achieve low biosurfactant productivity and yield and substrates are expensive [6]. Foam formation can cause serious operational issues and downstream biosurfactant recovery can be technically involved and costly. Development work to optimise bioprocesses should focus on enhancing biosurfactant yield and potency. Approaches include the search and discovery of novel biosurfactant-producing organisms and strain improvement by various genetic engineering methods and/or stress-fermentation including co-cultivation [84]. Yield can also be enhanced through the optimisation of culture conditions and costs reduced through the introduction of renewable or waste products [6, 28, 77] as cheaper feed stocks. The effects of biosurfactants on human health and the environment also require further assessment to ensure safe production and use.
6. Conclusions
Biosurfactants offer an attractive proposition for biotechnological application across various sectors and are considered superior to synthetic surfactants. Diverse MACA produce biosurfactants with interesting properties that have been explored in the context of biomedicine and environmental remediation. However, many MACA have not yet been investigated for biosurfactant production and various potential applications are yet to receive significant research. Rapid, reliable methods for high throughput screening for biosurfactant production are essential as are robust standard methods for biosurfactant purification and characterisation. Efforts to evaluate and expand the knowledge of structural characteristics and gene regulation of biosurfactants are warranted to improve their effectiveness and productivity. Commercial-scale production will need to employ various existing and new strategies to become economic and sustainable. Cutting-edge technologies such high-throughput omics-based tools should accelerate the development of commercial production of biosurfactants. Furthering our understanding of biosurfactants produced by MACA will facilitate their commercial exploitation thereby contributing to a sustainable bio-based economy.
Conflict of interest
The authors declare that there is no conflict of interest.
\n',keywords:"actinobacteria, antimicrobial, bioemulsifiers, bioremediation, biosurfactants, biotechnology, Corynebacteriales, mycolic acids, Rhodococcus",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/81978.pdf",chapterXML:"https://mts.intechopen.com/source/xml/81978.xml",downloadPdfUrl:"/chapter/pdf-download/81978",previewPdfUrl:"/chapter/pdf-preview/81978",totalDownloads:6,totalViews:0,totalCrossrefCites:0,dateSubmitted:"October 19th 2021",dateReviewed:"March 21st 2022",datePrePublished:"May 27th 2022",datePublished:null,dateFinished:"May 27th 2022",readingETA:"0",abstract:"The Actinobacteria produce an array of valuable metabolites including biosurfactants which are gaining increased attention in the biotechnology industries as they are multifunctional, biorenewable and generally superior to chemically synthesized compounds. Biosurfactants are surface-active, amphipathic molecules present at the microbial cell-surface or released extracellularly and in a variety of chemical forms. The mycolic acid-containing actinobacteria (MACA), classified in the order Corynebacteriales, represent a potentially rich source of biosurfactants for novel applications and undiscovered biosurfactant compounds. Members of the mycolate genus Rhodococcus produce various well-characterised glycolipids. However, other mycolate genera including Corynebacterium, Dietzia, Gordonia and Tsukamurella although less extensively investigated also possess biosurfactant-producing strains. This chapter captures current knowledge on biosurfactant production amongst the MACA, including their chemical structures and producer organisms. It also provides an overview of approaches to the recovery of biosurfactant producing MACA from the environment and assays available to screen for biosurfactant production. Methodologies applied in the extraction, purification, and structural elucidation of the different types of biosurfactants are also summarised. Potential future applications of MACA-derived biosurfactants are highlighted with particular focus on biomedical and environmental possibilities. Further investigation of biosurfactant production by MACA will enable the discovery of both novel producing strains and compounds with the prospect of biotechnological exploitation.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/81978",risUrl:"/chapter/ris/81978",signatures:"Fiona M. Stainsby, Janki Hodar and Halina Vaughan",book:{id:"10893",type:"book",title:"Actinobacteria",subtitle:null,fullTitle:"Actinobacteria",slug:null,publishedDate:null,bookSignature:"Prof. Wael N. Nabil Hozzein",coverURL:"https://cdn.intechopen.com/books/images_new/10893.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-80355-097-8",printIsbn:"978-1-80355-096-1",pdfIsbn:"978-1-80355-098-5",isAvailableForWebshopOrdering:!0,editors:[{id:"189233",title:"Prof.",name:"Wael N.",middleName:"Nabil",surname:"Hozzein",slug:"wael-n.-hozzein",fullName:"Wael N. Hozzein"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_1_2",title:"1.1 Biosurfactant properties",level:"2"},{id:"sec_2_2",title:"1.2 Mycolic acid-containing actinobacteria",level:"2"},{id:"sec_4",title:"2. Biosurfactants produced by MACA",level:"1"},{id:"sec_5",title:"3. Habitats, recovery, and growth requirements of MACA",level:"1"},{id:"sec_6",title:"4. Detection and characterisation of biosurfactants",level:"1"},{id:"sec_6_2",title:"4.1 Biosurfactant screening methods",level:"2"},{id:"sec_7_2",title:"4.2 Extraction and structural analysis of biosurfactants",level:"2"},{id:"sec_9",title:"5. Potential applications of biosurfactants from MACA",level:"1"},{id:"sec_9_2",title:"5.1 Biomedical applications",level:"2"},{id:"sec_10_2",title:"5.2 Environmental applications",level:"2"},{id:"sec_11_2",title:"5.3 Challenges to commercialisation",level:"2"},{id:"sec_13",title:"6. Conclusions",level:"1"},{id:"sec_17",title:"Conflict of interest",level:"1"}],chapterReferences:[{id:"B1",body:'Mnif I, Ghribi D. Lipopeptides biosurfactants: Mean classes and new insights for industrial, biomedical, and environmental applications. Biopolymers. 2015;104:129-147. DOI: 0.1002/bip.22630'},{id:"B2",body:'Goodfellow M, Jones AL. Corynebacteriales ord. nov. In: Whitman WB, editor. Bergey’s Manual of Systematics of Archaea and Bacteria. New Jersey: Wiley; 2015. p. 14. DOI: 10.1002/9781118960608.obm00009'},{id:"B3",body:'Bognolo G. Biosurfactants as emulsifying agents for hydrocarbons. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 1999;152(1-2):41-52. 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Department of Life Sciences, School of Applied Sciences, Edinburgh Napier University, Edinburgh, UK
Department of Life Sciences, School of Applied Sciences, Edinburgh Napier University, Edinburgh, UK
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IntechOpen’s Academic Editors and Authors have received funding for their work through many well-known funders, including: the European Commission, Bill and Melinda Gates Foundation, Wellcome Trust, Chinese Academy of Sciences, Natural Science Foundation of China (NSFC), CGIAR Consortium of International Agricultural Research Centers, National Institute of Health (NIH), National Science Foundation (NSF), National Aeronautics and Space Administration (NASA), National Institute of Standards and Technology (NIST), German Research Foundation (DFG), Research Councils United Kingdom (RCUK), Oswaldo Cruz Foundation, Austrian Science Fund (FWF), Foundation for Science and Technology (FCT), Australian Research Council (ARC).
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Does your institution already have a budget for covering Open Access publication costs?
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If you are associated with any of the institutions in our list below, you can apply to receive OA publication funds by following the instructions provided in the links. Please consult the Open Access policies or grant Terms and Conditions of any institution with which you are linked to explore ways to cover your publication costs (also accessible by clicking on the link in their title).
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Please be aware that you must be a member, or grantee, of the institutions/funders listed in order to apply for their Open Access publication funds.
Open Access publication costs can often be designated directly in the grants or in specific budgets allocated for that purpose. Many of the most important funding organisations encourage, and even request, that the projects they fund are made available at no cost to the wider public. IntechOpen strives to maintain excellent relationships with these funders and ensures compliance with mandates.
\n\n
In order to help Authors identify appropriate funding agencies and institutions, we have created a list, based on extensive research on various OA resources (including ROARMAP and SHERPA/JULIET) of organizations that have funds available. Before consulting our list we encourage you to petition your own institution or organization for Open Access funds or check the specifications of your grant with your funder to ascertain if publication costs are included. Where you are in receipt of a grant you should clarify:
\n\n
\n\t
Does your institution already have a budget for covering Open Access publication costs?
\n\t
Does your grant list Open Access publication fees as legitimate direct/indirect costs?
\n
\n\n
If you are associated with any of the institutions in our list below, you can apply to receive OA publication funds by following the instructions provided in the links. Please consult the Open Access policies or grant Terms and Conditions of any institution with which you are linked to explore ways to cover your publication costs (also accessible by clicking on the link in their title).
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The aim of the chapter is to give details on advance computational modelling and analytical methodologies, which can be used in order to design shallow and deep tunnels and to present real case studies from around the world, from very shallow tunnels in India with only 4.5 m overburden to a deep tunnel in Venezuela with extreme squeezing conditions under 1300 m overburden.",book:{id:"7690",slug:"tunnel-engineering-selected-topics",title:"Tunnel Engineering",fullTitle:"Tunnel Engineering - Selected Topics"},signatures:"Spiros Massinas",authors:[{id:"295762",title:"Dr.",name:"Spiros",middleName:null,surname:"Massinas",slug:"spiros-massinas",fullName:"Spiros Massinas"}]},{id:"68157",title:"Introductory Chapter: Textile Manufacturing Processes",slug:"introductory-chapter-textile-manufacturing-processes",totalDownloads:4414,totalCrossrefCites:13,totalDimensionsCites:24,abstract:null,book:{id:"8892",slug:"textile-manufacturing-processes",title:"Textile Manufacturing Processes",fullTitle:"Textile Manufacturing Processes"},signatures:"Faheem Uddin",authors:[{id:"228107",title:"Prof.",name:"Faheem",middleName:null,surname:"Uddin",slug:"faheem-uddin",fullName:"Faheem Uddin"}]},{id:"66828",title:"Breathing Monitoring and Pattern Recognition with Wearable Sensors",slug:"breathing-monitoring-and-pattern-recognition-with-wearable-sensors",totalDownloads:3062,totalCrossrefCites:10,totalDimensionsCites:13,abstract:"This chapter introduces the anatomy and physiology of the respiratory system, and the reasons for measuring breathing events, particularly, using wearable sensors. Respiratory monitoring is vital including detection of sleep apnea and measurement of respiratory rate. The automatic detection of breathing patterns is equally important in other respiratory rehabilitation therapies, for example, magnetic resonance exams for respiratory triggered imaging, and synchronized functional electrical stimulation. In this context, the goal of many research groups is to create wearable devices able to monitor breathing activity continuously, under natural physiological conditions in different environments. Therefore, wearable sensors that have been used recently as well as the main signal processing methods for breathing analysis are discussed. The following sensor technologies are presented: acoustic, resistive, inductive, humidity, acceleration, pressure, electromyography, impedance, and infrared. New technologies open the door to future methods of noninvasive breathing analysis using wearable sensors associated with machine learning techniques for pattern detection.",book:{id:"7654",slug:"wearable-devices-the-big-wave-of-innovation",title:"Wearable Devices",fullTitle:"Wearable Devices - the Big Wave of Innovation"},signatures:"Taisa Daiana da Costa, Maria de Fatima Fernandes Vara, Camila Santos Cristino, Tyene Zoraski Zanella, Guilherme Nunes Nogueira Neto and Percy Nohama",authors:[{id:"192464",title:"Ph.D.",name:"Percy",middleName:null,surname:"Nohama",slug:"percy-nohama",fullName:"Percy Nohama"},{id:"285706",title:"MSc.",name:"Taísa Daiana",middleName:null,surname:"Da Costa",slug:"taisa-daiana-da-costa",fullName:"Taísa Daiana Da Costa"},{id:"285707",title:"MSc.",name:"Maria de Fatima Fernandes",middleName:null,surname:"Vara",slug:"maria-de-fatima-fernandes-vara",fullName:"Maria de Fatima Fernandes Vara"},{id:"285708",title:"BSc.",name:"Camila Santos",middleName:null,surname:"Cristino",slug:"camila-santos-cristino",fullName:"Camila Santos Cristino"},{id:"285709",title:"Prof.",name:"Guilherme Nunes",middleName:null,surname:"Nogueira Neto",slug:"guilherme-nunes-nogueira-neto",fullName:"Guilherme Nunes Nogueira Neto"},{id:"293109",title:"BSc.",name:"Tyene",middleName:null,surname:"Zoraski Zanella",slug:"tyene-zoraski-zanella",fullName:"Tyene Zoraski Zanella"}]},{id:"41411",title:"Textile Dyes: Dyeing Process and Environmental Impact",slug:"textile-dyes-dyeing-process-and-environmental-impact",totalDownloads:20608,totalCrossrefCites:97,totalDimensionsCites:305,abstract:null,book:{id:"3137",slug:"eco-friendly-textile-dyeing-and-finishing",title:"Eco-Friendly Textile Dyeing and Finishing",fullTitle:"Eco-Friendly Textile Dyeing and Finishing"},signatures:"Farah Maria Drumond Chequer, Gisele Augusto Rodrigues de Oliveira, Elisa Raquel Anastácio Ferraz, Juliano Carvalho Cardoso, Maria Valnice Boldrin Zanoni and Danielle Palma de Oliveira",authors:[{id:"49040",title:"Prof.",name:"Danielle",middleName:null,surname:"Palma De Oliveira",slug:"danielle-palma-de-oliveira",fullName:"Danielle Palma De Oliveira"},{id:"149074",title:"Prof.",name:"Maria Valnice",middleName:null,surname:"Zanoni",slug:"maria-valnice-zanoni",fullName:"Maria Valnice Zanoni"},{id:"153502",title:"Ph.D.",name:"Farah",middleName:null,surname:"Chequer",slug:"farah-chequer",fullName:"Farah Chequer"},{id:"153504",title:"MSc.",name:"Gisele",middleName:null,surname:"Oliveira",slug:"gisele-oliveira",fullName:"Gisele Oliveira"},{id:"163377",title:"Dr.",name:"Juliano",middleName:null,surname:"Cardoso",slug:"juliano-cardoso",fullName:"Juliano Cardoso"},{id:"163393",title:"Dr.",name:"Elisa",middleName:null,surname:"Ferraz",slug:"elisa-ferraz",fullName:"Elisa Ferraz"}]},{id:"70242",title:"Advancements in the Fenton Process for Wastewater Treatment",slug:"advancements-in-the-fenton-process-for-wastewater-treatment",totalDownloads:1925,totalCrossrefCites:11,totalDimensionsCites:24,abstract:"Fenton is considered to be one of the most effective advanced treatment processes in the removal of many hazardous organic pollutants from refractory/toxic wastewater. It has many advantages, but drawbacks are significant such as a strong acid environment, the cost of reagents consumption, and the large production of ferric sludge, which limits Fenton’s further application. The development of Fenton applications is mainly achieved by improving oxidation efficiency and reducing sludge production. This chapter presents a review on fundamentals and applications of conventional Fenton, leading advanced technologies in the Fenton process, and reuse methods of iron containing sludge to synthetic and real wastewaters are discussed. Finally, future trends and some guidelines for Fenton processes are given.",book:{id:"9415",slug:"advanced-oxidation-processes-applications-trends-and-prospects",title:"Advanced Oxidation Processes",fullTitle:"Advanced Oxidation Processes - Applications, Trends, and Prospects"},signatures:"Min Xu, Changyong Wu and Yuexi Zhou",authors:[{id:"307479",title:"Dr.",name:"Changyong",middleName:null,surname:"Wu",slug:"changyong-wu",fullName:"Changyong Wu"},{id:"307546",title:"Prof.",name:"Yuexi",middleName:null,surname:"Zhou",slug:"yuexi-zhou",fullName:"Yuexi Zhou"},{id:"311139",title:"Dr.",name:"Min",middleName:null,surname:"Xu",slug:"min-xu",fullName:"Min Xu"}]}],onlineFirstChaptersFilter:{topicId:"24",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"79832",title:"Multiplexing, Transmission and De-Multiplexing of OAM Modes through Specialty Fibers",slug:"multiplexing-transmission-and-de-multiplexing-of-oam-modes-through-specialty-fibers",totalDownloads:2,totalDimensionsCites:0,doi:"10.5772/intechopen.101340",abstract:"Space division multiplexing (SDM) over fibers has introduced a new paradigm in optical communication thanks to its capability to meet the ever-renewed demand of more transmission capacity and on large spectral efficiency. This ever-increasing demand is pushed by the nonstop increase of the number of connected users, devices, processes, and data (toward internet of everything IOE). One of the most promising variants of SDM, that has recently shown great potential, is based on harnessing orbital angular momentum (OAM) modes as data carriers. These OAMs are multiplexed, transmitted over special optical fibers (OAM-fibers) then de-multiplexed. In order to highlight the potential of SDM system incorporating OAM modes through fibers, in this chapter, we disassemble an SDM system and we examine its main key elements. The potential of OAM-SDM is discussed as a promising candidate for the next generation local/global communications networks. This chapter is intended to provide a comprehensive and deep understanding of SDM, which will push R&D community to derive future research directions in the field.",book:{id:"10990",title:"Multiplexing - Recent Advances and Novel Applications",coverURL:"https://cdn.intechopen.com/books/images_new/10990.jpg"},signatures:"Alaaeddine Rjeb, Habib Fathallah and Mohsen Machhout"},{id:"82443",title:"Phase Noise in OFDM",slug:"phase-noise-in-ofdm",totalDownloads:2,totalDimensionsCites:0,doi:"10.5772/intechopen.105551",abstract:"Orthogonal frequency division multiplexing (OFDM) technique provides high data rate with high spectral efficiency for operating close to the Shanon capacity bounds. With the advantages of simple channel equalization, robustness against frequency selectivity of the channel, and efficient implementation, this is a widely deployed technique. Orthogonal frequency division multiplexing access (OFDMA), the multiple access technique using OFDM, has the great potential for providing high spectral efficiency due to its integrated space-frequency and multiuser diversity. Besides all the advantages, OFDM/A is very susceptible to transceiver’s impairments such as phase noise (PHN), carrier frequency offset, and in-quadrature phase imbalance effect. Phase noise is the random fluctuation in phase of the sinusoidal waveform used for frequency up/down conversion of baseband signals to/from RF (radio frequency). This occurs due to the inherent imperfections of oscillators used for this purpose. This chapter addresses the orthogonal frequency division multiplexing/multiple access system performance under the impact of transceiver oscillator phase noise.",book:{id:"10990",title:"Multiplexing - Recent Advances and Novel Applications",coverURL:"https://cdn.intechopen.com/books/images_new/10990.jpg"},signatures:"Kamayani Shrivastav"},{id:"82089",title:"Perspective Chapter: Breaking the Barriers – Additive Technologies (AX) for Integrated Process Chains and Integrated Devices (IDs) for Hybrid Product Architectures",slug:"perspective-chapter-breaking-the-barriers-additive-technologies-ax-for-integrated-process-chains-and",totalDownloads:18,totalDimensionsCites:0,doi:"10.5772/intechopen.104891",abstract:"Additive technology has evolved from rapid prototyping to rapid tooling and manufacturing of load-bearing parts for productive use. Application potential is limited by constituent strengths and weaknesses. To enfold its full potential, research, development, and industrial application have to facilitate combinations of additive and conventional technology. The concept of additive parts manufacturing has to be expanded to a mature technology contributing and facilitating hybrid products and integrated process chains. From a two-dimensional reference model, approaches to integration are derived, and their status is briefly outlined: Efforts to facilitate postprocessing by design for additive manufacturing (DfAM) and hybrid manufacturing have been raised to awareness and are being worked on. Yet, integration of pre-fabricated structures is hardly accounted for, although it bears the potential for a paradigmatic shift in manufacturing: With a wider concept of layer-based processes, Additive Technology could form the core technology for integration of components and functions to Integrated Devices, following the model of the Integrated Circuits and packaging technology in microelectronics and Microelectromechanical Systems. First developments are outlined, but research and development effort has to be dedicated to novel additive processes for this application. Finally, workflows for product developers need to be modified and trained to plan hybrid product architectures already in conceptual phases.",book:{id:"10974",title:"Advanced Additive Manufacturing",coverURL:"https://cdn.intechopen.com/books/images_new/10974.jpg"},signatures:"Matthias Dahlmeyer and Sebastian Noller"},{id:"81952",title:"Perspective Chapter: Multi-Material in 3D Printing for Engineering Applications",slug:"perspective-chapter-multi-material-in-3d-printing-for-engineering-applications",totalDownloads:23,totalDimensionsCites:0,doi:"10.5772/intechopen.102564",abstract:"3D Printing or Additive Manufacturing is one of a novel method in manufacturing of materials with increased accuracy of manufacturing in terms of complexity in parts, design of aerospace and defense parts, light-weighting, etc., This manufacturing method involves layer-by-layer printing or deposition of materials or metals into the perfectly aligned especially in corners, edges and in most complex designs. The design process mostly involved software so that production cost could be estimated in the design stage itself. Additive Manufacturing is one of the most promising approach for small and low-volume productions. The filament used for the process is prominent to the designer, along with the various printing processes. Recent modern printing techniques involve multiple nozzles, whereas designers can use multiple materials on single printing. The use of multi-material in a single part enables the manufacturer to rapidly produce products which have specific applications. This chapter discusses about various multi-material with different mechanical properties that can be used for structural applications through different printing technologies on various precious applications. This technology is quickly adopted by even small-scale industries in recent times.",book:{id:"10974",title:"Advanced Additive Manufacturing",coverURL:"https://cdn.intechopen.com/books/images_new/10974.jpg"},signatures:"Rajkumar Velu, R. Sathishkumar and A. Saiyathibrahim"},{id:"81932",title:"Waveguide Amplifier for Extended Reach of WDM/FSO",slug:"waveguide-amplifier-for-extended-reach-of-wdm-fso",totalDownloads:5,totalDimensionsCites:0,doi:"10.5772/intechopen.104790",abstract:"In this chapter, EYDWA (erbium ytterbium doped waveguide amplifier) is characterized for wavelength division multiplexing (WDM) approach on free space optical (FSO) transmission systems with channels being spaced at 0.4 nm interval. Moreover, in this paper, was study different characterizations of EYDWA amplifier, which depend essentially on the opt-geometric parameters, such as concentrations of ions erbium, length of the waveguide and the effect of those parameters to optimize the performance of proposed system. Furthermore, the results reveal that the EYDWA booster (post-amplification) can improve the high performance remarkably under clear rain and the acceptable transmission can be carried out up to 26 km while it get reduced to 19.5 km by using pre-amplification.",book:{id:"10990",title:"Multiplexing - Recent Advances and Novel Applications",coverURL:"https://cdn.intechopen.com/books/images_new/10990.jpg"},signatures:"Bentahar Attaouia, Kandouci Malika, Ghouali Samir and Dinar Amina Elbatoul"},{id:"80282",title:"Modeling of LPBF Scanning Strategy and its Correlation with the Metallic 316 L, 321, and Alnico Magnets Samples Structure",slug:"modeling-of-lpbf-scanning-strategy-and-its-correlation-with-the-metallic-316-l-321-and-alnico-magnet",totalDownloads:17,totalDimensionsCites:0,doi:"10.5772/intechopen.102073",abstract:"This chapter presents the influence of powder bed laser scanning strategy on the crystallographic structure of the fused specimens 316 L, 321 stainless steel, and Alnico magnets. The main parameters affecting structure are as follows—laser power, stripe width, number of repeated passes with different power, and type of scanning (circle, bidirectional or interlaced, etc.). Changes in the crystallographic structure are studied with regard to melt pool geometry, surface temperature, and surface heat transfer. The correlation is shown between stripe width and laser beam focal spot diameter. Depending on the ratio between stripe width and laser beam focal spot diameter one can see growth elongated and oriented grains or quasi-equiaxed non-oriented grains. The influence of the energy input on the melt pool size and the microstructure of the sample is studied. The influence of the scanning mode (bidirectional and circular) on the temperature distribution in the sample and the microstructure of the sample made of Alnico alloy is considered. All these experimental and model examples clearly demonstrate that it is possible to produce a controllable structure during LPBF process building for advanced additive manufacturing.",book:{id:"10974",title:"Advanced Additive Manufacturing",coverURL:"https://cdn.intechopen.com/books/images_new/10974.jpg"},signatures:"Pavel Kuznetsov, Anna Mozhayko, Ivan Shakirov, Vitaliy Bobyr, Mikhail Staritsyn and Anton Zhukov"}],onlineFirstChaptersTotal:19},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:0,limit:8,total:null},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:9,numberOfPublishedChapters:89,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:104,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:32,numberOfPublishedChapters:318,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:12,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:141,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:113,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:5,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:15,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}}]},series:{item:{id:"11",title:"Biochemistry",doi:"10.5772/intechopen.72877",issn:"2632-0983",scope:"Biochemistry, the study of chemical transformations occurring within living organisms, impacts all areas of life sciences, from molecular crystallography and genetics to ecology, medicine, and population biology. Biochemistry examines macromolecules - proteins, nucleic acids, carbohydrates, and lipids – and their building blocks, structures, functions, and interactions. Much of biochemistry is devoted to enzymes, proteins that catalyze chemical reactions, enzyme structures, mechanisms of action and their roles within cells. Biochemistry also studies small signaling molecules, coenzymes, inhibitors, vitamins, and hormones, which play roles in life processes. Biochemical experimentation, besides coopting classical chemistry methods, e.g., chromatography, adopted new techniques, e.g., X-ray diffraction, electron microscopy, NMR, radioisotopes, and developed sophisticated microbial genetic tools, e.g., auxotroph mutants and their revertants, fermentation, etc. More recently, biochemistry embraced the ‘big data’ omics systems. Initial biochemical studies have been exclusively analytic: dissecting, purifying, and examining individual components of a biological system; in the apt words of Efraim Racker (1913 –1991), “Don’t waste clean thinking on dirty enzymes.” Today, however, biochemistry is becoming more agglomerative and comprehensive, setting out to integrate and describe entirely particular biological systems. The ‘big data’ metabolomics can define the complement of small molecules, e.g., in a soil or biofilm sample; proteomics can distinguish all the comprising proteins, e.g., serum; metagenomics can identify all the genes in a complex environment, e.g., the bovine rumen. This Biochemistry Series will address the current research on biomolecules and the emerging trends with great promise.",coverUrl:"https://cdn.intechopen.com/series/covers/11.jpg",latestPublicationDate:"June 29th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:32,editor:{id:"31610",title:"Dr.",name:"Miroslav",middleName:null,surname:"Blumenberg",slug:"miroslav-blumenberg",fullName:"Miroslav Blumenberg",profilePictureURL:"https://mts.intechopen.com/storage/users/31610/images/system/31610.jpg",biography:"Miroslav Blumenberg, Ph.D., was born in Subotica and received his BSc in Belgrade, Yugoslavia. He completed his Ph.D. at MIT in Organic Chemistry; he followed up his Ph.D. with two postdoctoral study periods at Stanford University. Since 1983, he has been a faculty member of the RO Perelman Department of Dermatology, NYU School of Medicine, where he is codirector of a training grant in cutaneous biology. Dr. Blumenberg’s research is focused on the epidermis, expression of keratin genes, transcription profiling, keratinocyte differentiation, inflammatory diseases and cancers, and most recently the effects of the microbiome on the skin. He has published more than 100 peer-reviewed research articles and graduated numerous Ph.D. and postdoctoral students.",institutionString:null,institution:{name:"New York University Langone Medical Center",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:4,paginationItems:[{id:"14",title:"Cell and Molecular Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/14.jpg",isOpenForSubmission:!0,editor:{id:"165627",title:"Dr.",name:"Rosa María",middleName:null,surname:"Martínez-Espinosa",slug:"rosa-maria-martinez-espinosa",fullName:"Rosa María Martínez-Espinosa",profilePictureURL:"https://mts.intechopen.com/storage/users/165627/images/system/165627.jpeg",biography:"Dr. Rosa María Martínez-Espinosa has been a Spanish Full Professor since 2020 (Biochemistry and Molecular Biology) and is currently Vice-President of International Relations and Cooperation development and leader of the research group 'Applied Biochemistry” (University of Alicante, Spain). Other positions she has held at the university include Vice-Dean of Master Programs, Vice-Dean of the Degree in Biology and Vice-Dean for Mobility and Enterprise and Engagement at the Faculty of Science (University of Alicante). She received her Bachelor in Biology in 1998 (University of Alicante) and her PhD in 2003 (Biochemistry, University of Alicante). She undertook post-doctoral research at the University of East Anglia (Norwich, U.K. 2004-2005; 2007-2008).\nHer multidisciplinary research focuses on investigating archaea and their potential applications in biotechnology. She has an H-index of 21. She has authored one patent and has published more than 70 indexed papers and around 60 book chapters.\nShe has contributed to more than 150 national and international meetings during the last 15 years. Her research interests include archaea metabolism, enzymes purification and characterization, gene regulation, carotenoids and bioplastics production, antioxidant\ncompounds, waste water treatments, and brines bioremediation.\nRosa María’s other roles include editorial board member for several journals related\nto biochemistry, reviewer for more than 60 journals (biochemistry, molecular biology, biotechnology, chemistry and microbiology) and president of several organizing committees in international meetings related to the N-cycle or respiratory processes.",institutionString:null,institution:{name:"University of Alicante",institutionURL:null,country:{name:"Spain"}}},editorTwo:null,editorThree:null},{id:"15",title:"Chemical Biology",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",isOpenForSubmission:!0,editor:{id:"441442",title:"Dr.",name:"Şükrü",middleName:null,surname:"Beydemir",slug:"sukru-beydemir",fullName:"Şükrü Beydemir",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y00003GsUoIQAV/Profile_Picture_1634557147521",biography:"Dr. Şükrü Beydemir obtained a BSc in Chemistry in 1995 from Yüzüncü Yıl University, MSc in Biochemistry in 1998, and PhD in Biochemistry in 2002 from Atatürk University, Turkey. He performed post-doctoral studies at Max-Planck Institute, Germany, and University of Florence, Italy in addition to making several scientific visits abroad. He currently works as a Full Professor of Biochemistry in the Faculty of Pharmacy, Anadolu University, Turkey. Dr. Beydemir has published over a hundred scientific papers spanning protein biochemistry, enzymology and medicinal chemistry, reviews, book chapters and presented several conferences to scientists worldwide. He has received numerous publication awards from various international scientific councils. He serves in the Editorial Board of several international journals. Dr. Beydemir is also Rector of Bilecik Şeyh Edebali University, Turkey.",institutionString:null,institution:{name:"Anadolu University",institutionURL:null,country:{name:"Turkey"}}},editorTwo:{id:"13652",title:"Prof.",name:"Deniz",middleName:null,surname:"Ekinci",slug:"deniz-ekinci",fullName:"Deniz Ekinci",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYLT1QAO/Profile_Picture_1634557223079",biography:"Dr. Deniz Ekinci obtained a BSc in Chemistry in 2004, MSc in Biochemistry in 2006, and PhD in Biochemistry in 2009 from Atatürk University, Turkey. He studied at Stetson University, USA, in 2007-2008 and at the Max Planck Institute of Molecular Cell Biology and Genetics, Germany, in 2009-2010. Dr. Ekinci currently works as a Full Professor of Biochemistry in the Faculty of Agriculture and is the Head of the Enzyme and Microbial Biotechnology Division, Ondokuz Mayıs University, Turkey. He is a member of the Turkish Biochemical Society, American Chemical Society, and German Genetics society. Dr. Ekinci published around ninety scientific papers, reviews and book chapters, and presented several conferences to scientists. He has received numerous publication awards from several scientific councils. Dr. Ekinci serves as the Editor in Chief of four international books and is involved in the Editorial Board of several international journals.",institutionString:null,institution:{name:"Ondokuz Mayıs University",institutionURL:null,country:{name:"Turkey"}}},editorThree:null},{id:"17",title:"Metabolism",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",isOpenForSubmission:!0,editor:{id:"138626",title:"Dr.",name:"Yannis",middleName:null,surname:"Karamanos",slug:"yannis-karamanos",fullName:"Yannis Karamanos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002g6Jv2QAE/Profile_Picture_1629356660984",biography:"Yannis Karamanos, born in Greece in 1953, completed his pre-graduate studies at the Université Pierre et Marie Curie, Paris, then his Masters and Doctoral degree at the Université de Lille (1983). He was associate professor at the University of Limoges (1987) before becoming full professor of biochemistry at the Université d’Artois (1996). He worked on the structure-function relationships of glycoconjugates and his main project was the investigations on the biological roles of the de-N-glycosylation enzymes (Endo-N-acetyl-β-D-glucosaminidase and peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase). From 2002 he contributes to the understanding of the Blood-brain barrier functioning using proteomics approaches. He has published more than 70 papers. 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Since then, he has been working as an Adjunct Professor in the same Department at the University of Pavia. His research activity during the first years was primarily focused on the purification and structural characterization of enzymes from animal and plant sources. During this period, Prof. Iadarola familiarized himself with the conventional techniques used in column chromatography, spectrophotometry, manual Edman degradation, and electrophoresis). Since 1995, he has been working on: i) the determination in biological fluids (serum, urine, bronchoalveolar lavage, sputum) of proteolytic activities involved in the degradation processes of connective tissue matrix, and ii) on the identification of biological markers of lung diseases. In this context, he has developed and validated new methodologies (e.g., Capillary Electrophoresis coupled to Laser-Induced Fluorescence, CE-LIF) whose application enabled him to determine both the amounts of biochemical markers (Desmosines) in urine/serum of patients affected by Chronic Obstructive Pulmonary Disease (COPD) and the activity of proteolytic enzymes (Human Neutrophil Elastase, Cathepsin G, Pseudomonas aeruginosa elastase) in sputa of these patients. More recently, Prof. Iadarola was involved in developing techniques such as two-dimensional electrophoresis coupled to liquid chromatography/mass spectrometry (2DE-LC/MS) for the proteomic analysis of biological fluids aimed at the identification of potential biomarkers of different lung diseases. He is the author of about 150 publications (According to Scopus: H-Index: 23; Total citations: 1568- According to WOS: H-Index: 20; Total Citations: 1296) of peer-reviewed international journals. 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She is now a lecturer at the University of Witwatersrand, South Africa, and a principal researcher at the Health Economics and Epidemiology Research Office (HE2RO), South Africa. Dr. Moolla holds a Ph.D. in Psychology with her research being focused on mental health and resilience. In her professional work capacity, her research has further expanded into the fields of early childhood development, mental health, the HIV and TB care cascades, as well as COVID. She is also a UNESCO-trained International Bioethics Facilitator.",institutionString:"University of the Witwatersrand",institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419588",title:"Ph.D.",name:"Sergio",middleName:"Alexandre",surname:"Gehrke",slug:"sergio-gehrke",fullName:"Sergio Gehrke",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038WgMKQA0/Profile_Picture_2022-06-02T11:44:20.jpg",biography:"Dr. Sergio Alexandre Gehrke is a doctorate holder in two fields. The first is a Ph.D. in Cellular and Molecular Biology from the Pontificia Catholic University, Porto Alegre, Brazil, in 2010 and the other is an International Ph.D. in Bioengineering from the Universidad Miguel Hernandez, Elche/Alicante, Spain, obtained in 2020. In 2018, he completed a postdoctoral fellowship in Materials Engineering in the NUCLEMAT of the Pontificia Catholic University, Porto Alegre, Brazil. He is currently the Director of the Postgraduate Program in Implantology of the Bioface/UCAM/PgO (Montevideo, Uruguay), Director of the Cathedra of Biotechnology of the Catholic University of Murcia (Murcia, Spain), an Extraordinary Full Professor of the Catholic University of Murcia (Murcia, Spain) as well as the Director of the private center of research Biotecnos – Technology and Science (Montevideo, Uruguay). Applied biomaterials, cellular and molecular biology, and dental implants are among his research interests. He has published several original papers in renowned journals. In addition, he is also a Collaborating Professor in several Postgraduate programs at different universities all over the world.",institutionString:null,institution:{name:"Universidad Católica San Antonio de Murcia",country:{name:"Spain"}}},{id:"342152",title:"Dr.",name:"Santo",middleName:null,surname:"Grace Umesh",slug:"santo-grace-umesh",fullName:"Santo Grace Umesh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/342152/images/16311_n.jpg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"333647",title:"Dr.",name:"Shreya",middleName:null,surname:"Kishore",slug:"shreya-kishore",fullName:"Shreya Kishore",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333647/images/14701_n.jpg",biography:"Dr. Shreya Kishore completed her Bachelor in Dental Surgery in Chettinad Dental College and Research Institute, Chennai, and her Master of Dental Surgery (Orthodontics) in Saveetha Dental College, Chennai. She is also Invisalign certified. She’s working as a Senior Lecturer in the Department of Orthodontics, SRM Dental College since November 2019. She is actively involved in teaching orthodontics to the undergraduates and the postgraduates. Her clinical research topics include new orthodontic brackets, fixed appliances and TADs. She’s published 4 articles in well renowned indexed journals and has a published patency of her own. Her private practice is currently limited to orthodontics and works as a consultant in various clinics.",institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"323731",title:"Prof.",name:"Deepak M.",middleName:"Macchindra",surname:"Vikhe",slug:"deepak-m.-vikhe",fullName:"Deepak M. Vikhe",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/323731/images/13613_n.jpg",biography:"Dr Deepak M.Vikhe .\n\n\t\n\tDr Deepak M.Vikhe , completed his Masters & PhD in Prosthodontics from Rural Dental College, Loni securing third rank in the Pravara Institute of Medical Sciences Deemed University. He was awarded Dr.G.C.DAS Memorial Award for Research on Implants at 39th IPS conference Dubai (U A E).He has two patents under his name. He has received Dr.Saraswati medal award for best research for implant study in 2017.He has received Fully funded scholarship to Spain ,university of Santiago de Compostela. He has completed fellowship in Implantlogy from Noble Biocare. \nHe has attended various conferences and CDE programmes and has national publications to his credit. His field of interest is in Implant supported prosthesis. Presently he is working as a associate professor in the Dept of Prosthodontics, Rural Dental College, Loni and maintains a successful private practice specialising in Implantology at Rahata.\n\nEmail: drdeepak_mvikhe@yahoo.com..................",institutionString:null,institution:{name:"Pravara Institute of Medical Sciences",country:{name:"India"}}},{id:"204110",title:"Dr.",name:"Ahmed A.",middleName:null,surname:"Madfa",slug:"ahmed-a.-madfa",fullName:"Ahmed A. Madfa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204110/images/system/204110.jpg",biography:"Dr. Madfa is currently Associate Professor of Endodontics at Thamar University and a visiting lecturer at Sana'a University and University of Sciences and Technology. He has more than 6 years of experience in teaching. His research interests include root canal morphology, functionally graded concept, dental biomaterials, epidemiology and dental education, biomimetic restoration, finite element analysis and endodontic regeneration. Dr. Madfa has numerous international publications, full articles, two patents, a book and a book chapter. Furthermore, he won 14 international scientific awards. Furthermore, he is involved in many academic activities ranging from editorial board member, reviewer for many international journals and postgraduate students' supervisor. Besides, I deliver many courses and training workshops at various scientific events. Dr. Madfa also regularly attends international conferences and holds administrative positions (Deputy Dean of the Faculty for Students’ & Academic Affairs and Deputy Head of Research Unit).",institutionString:"Thamar University",institution:null},{id:"210472",title:"Dr.",name:"Nermin",middleName:"Mohammed Ahmed",surname:"Yussif",slug:"nermin-yussif",fullName:"Nermin Yussif",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/210472/images/system/210472.jpg",biography:"Dr. Nermin Mohammed Ahmed Yussif is working at the Faculty of dentistry, University for October university for modern sciences and arts (MSA). Her areas of expertise include: periodontology, dental laserology, oral implantology, periodontal plastic surgeries, oral mesotherapy, nutrition, dental pharmacology. She is an editor and reviewer in numerous international journals.",institutionString:"MSA University",institution:null},{id:"204606",title:"Dr.",name:"Serdar",middleName:null,surname:"Gözler",slug:"serdar-gozler",fullName:"Serdar Gözler",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204606/images/system/204606.jpeg",biography:"Dr. Serdar Gözler has completed his undergraduate studies at the Marmara University Faculty of Dentistry in 1978, followed by an assistantship in the Prosthesis Department of Dicle University Faculty of Dentistry. Starting his PhD work on non-resilient overdentures with Assoc. Prof. Hüsnü Yavuzyılmaz, he continued his studies with Prof. Dr. Gürbüz Öztürk of Istanbul University Faculty of Dentistry Department of Prosthodontics, this time on Gnatology. He attended training programs on occlusion, neurology, neurophysiology, EMG, radiology and biostatistics. In 1982, he presented his PhD thesis \\Gerber and Lauritzen Occlusion Analysis Techniques: Diagnosis Values,\\ at Istanbul University School of Dentistry, Department of Prosthodontics. As he was also working with Prof. Senih Çalıkkocaoğlu on The Physiology of Chewing at the same time, Gözler has written a chapter in Çalıkkocaoğlu\\'s book \\Complete Prostheses\\ entitled \\The Place of Neuromuscular Mechanism in Prosthetic Dentistry.\\ The book was published five times since by the Istanbul University Publications. Having presented in various conferences about occlusion analysis until 1998, Dr. Gözler has also decided to use the T-Scan II occlusion analysis method. Having been personally trained by Dr. Robert Kerstein on this method, Dr. Gözler has been lecturing on the T-Scan Occlusion Analysis Method in conferences both in Turkey and abroad. Dr. Gözler has various articles and presentations on Digital Occlusion Analysis methods. He is now Head of the TMD Clinic at Prosthodontic Department of Faculty of Dentistry , Istanbul Aydın University , Turkey.",institutionString:"Istanbul Aydin University",institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"240870",title:"Ph.D.",name:"Alaa Eddin Omar",middleName:null,surname:"Al Ostwani",slug:"alaa-eddin-omar-al-ostwani",fullName:"Alaa Eddin Omar Al Ostwani",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/240870/images/system/240870.jpeg",biography:"Dr. Al Ostwani Alaa Eddin Omar received his Master in dentistry from Damascus University in 2010, and his Ph.D. in Pediatric Dentistry from Damascus University in 2014. Dr. Al Ostwani is an assistant professor and faculty member at IUST University since 2014. \nDuring his academic experience, he has received several awards including the scientific research award from the Union of Arab Universities, the Syrian gold medal and the international gold medal for invention and creativity. Dr. Al Ostwani is a Member of the International Association of Dental Traumatology and the Syrian Society for Research and Preventive Dentistry since 2017. He is also a Member of the Reviewer Board of International Journal of Dental Medicine (IJDM), and the Indian Journal of Conservative and Endodontics since 2016.",institutionString:"International University for Science and Technology.",institution:{name:"Islamic University of Science and Technology",country:{name:"India"}}},{id:"42847",title:"Dr.",name:"Belma",middleName:null,surname:"Işik Aslan",slug:"belma-isik-aslan",fullName:"Belma Işik Aslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/42847/images/system/42847.jpg",biography:"Dr. Belma IşIk Aslan was born in 1976 in Ankara-TURKEY. After graduating from TED Ankara College in 1994, she attended to Gazi University, Faculty of Dentistry in Ankara. She completed her PhD in orthodontic education at Gazi University between 1999-2005. Dr. Işık Aslan stayed at the Providence Hospital Craniofacial Institude and Reconstructive Surgery in Michigan, USA for three months as an observer. She worked as a specialist doctor at Gazi University, Dentistry Faculty, Department of Orthodontics between 2005-2014. She was appointed as associate professor in January, 2014 and as professor in 2021. Dr. Işık Aslan still works as an instructor at the same faculty. She has published a total of 35 articles, 10 book chapters, 39 conference proceedings both internationally and nationally. Also she was the academic editor of the international book 'Current Advances in Orthodontics'. She is a member of the Turkish Orthodontic Society and Turkish Cleft Lip and Palate Society. She is married and has 2 children. Her knowledge of English is at an advanced level.",institutionString:"Gazi University Dentistry Faculty Department of Orthodontics",institution:null},{id:"178412",title:"Associate Prof.",name:"Guhan",middleName:null,surname:"Dergin",slug:"guhan-dergin",fullName:"Guhan Dergin",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178412/images/6954_n.jpg",biography:"Assoc. Prof. Dr. Gühan Dergin was born in 1973 in Izmit. He graduated from Marmara University Faculty of Dentistry in 1999. He completed his specialty of OMFS surgery in Marmara University Faculty of Dentistry and obtained his PhD degree in 2006. In 2005, he was invited as a visiting doctor in the Oral and Maxillofacial Surgery Department of the University of North Carolina, USA, where he went on a scholarship. Dr. Dergin still continues his academic career as an associate professor in Marmara University Faculty of Dentistry. He has many articles in international and national scientific journals and chapters in books.",institutionString:null,institution:{name:"Marmara University",country:{name:"Turkey"}}},{id:"178414",title:"Prof.",name:"Yusuf",middleName:null,surname:"Emes",slug:"yusuf-emes",fullName:"Yusuf Emes",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178414/images/6953_n.jpg",biography:"Born in Istanbul in 1974, Dr. Emes graduated from Istanbul University Faculty of Dentistry in 1997 and completed his PhD degree in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery in 2005. He has papers published in international and national scientific journals, including research articles on implantology, oroantral fistulas, odontogenic cysts, and temporomandibular disorders. Dr. Emes is currently working as a full-time academic staff in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery.",institutionString:null,institution:{name:"Istanbul University",country:{name:"Turkey"}}},{id:"192229",title:"Ph.D.",name:"Ana Luiza",middleName:null,surname:"De Carvalho Felippini",slug:"ana-luiza-de-carvalho-felippini",fullName:"Ana Luiza De Carvalho Felippini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192229/images/system/192229.jpg",biography:null,institutionString:"University of São Paulo",institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"256851",title:"Prof.",name:"Ayşe",middleName:null,surname:"Gülşen",slug:"ayse-gulsen",fullName:"Ayşe Gülşen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256851/images/9696_n.jpg",biography:"Dr. Ayşe Gülşen graduated in 1990 from Faculty of Dentistry, University of Ankara and did a postgraduate program at University of Gazi. \nShe worked as an observer and research assistant in Craniofacial Surgery Departments in New York, Providence Hospital in Michigan and Chang Gung Memorial Hospital in Taiwan. \nShe works as Craniofacial Orthodontist in Department of Aesthetic, Plastic and Reconstructive Surgery, Faculty of Medicine, University of Gazi, Ankara Turkey since 2004.",institutionString:"Univeristy of Gazi",institution:null},{id:"255366",title:"Prof.",name:"Tosun",middleName:null,surname:"Tosun",slug:"tosun-tosun",fullName:"Tosun Tosun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255366/images/7347_n.jpg",biography:"Graduated at the Faculty of Dentistry, University of Istanbul, Turkey in 1989;\nVisitor Assistant at the University of Padua, Italy and Branemark Osseointegration Center of Treviso, Italy between 1993-94;\nPhD thesis on oral implantology in University of Istanbul and was awarded the academic title “Dr.med.dent.”, 1997;\nHe was awarded the academic title “Doç.Dr.” (Associated Professor) in 2003;\nProficiency in Botulinum Toxin Applications, Reading-UK in 2009;\nMastership, RWTH Certificate in Laser Therapy in Dentistry, AALZ-Aachen University, Germany 2009-11;\nMaster of Science (MSc) in Laser Dentistry, University of Genoa, Italy 2013-14.\n\nDr.Tosun worked as Research Assistant in the Department of Oral Implantology, Faculty of Dentistry, University of Istanbul between 1990-2002. \nHe worked part-time as Consultant surgeon in Harvard Medical International Hospitals and John Hopkins Medicine, Istanbul between years 2007-09.\u2028He was contract Professor in the Department of Surgical and Diagnostic Sciences (DI.S.C.), Medical School, University of Genova, Italy between years 2011-16. \nSince 2015 he is visiting Professor at Medical School, University of Plovdiv, Bulgaria. \nCurrently he is Associated Prof.Dr. at the Dental School, Oral Surgery Dept., Istanbul Aydin University and since 2003 he works in his own private clinic in Istanbul, Turkey.\u2028\nDr.Tosun is reviewer in journal ‘Laser in Medical Sciences’, reviewer in journal ‘Folia Medica\\', a Fellow of the International Team for Implantology, Clinical Lecturer of DGZI German Association of Oral Implantology, Expert Lecturer of Laser&Health Academy, Country Representative of World Federation for Laser Dentistry, member of European Federation of Periodontology, member of Academy of Laser Dentistry. Dr.Tosun presents papers in international and national congresses and has scientific publications in international and national journals. He speaks english, spanish, italian and french.",institutionString:null,institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"171887",title:"Prof.",name:"Zühre",middleName:null,surname:"Akarslan",slug:"zuhre-akarslan",fullName:"Zühre Akarslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/171887/images/system/171887.jpg",biography:"Zühre Akarslan was born in 1977 in Cyprus. She graduated from Gazi University Faculty of Dentistry, Ankara, Turkey in 2000. \r\nLater she received her Ph.D. degree from the Oral Diagnosis and Radiology Department; which was recently renamed as Oral and Dentomaxillofacial Radiology, from the same university. \r\nShe is working as a full-time Associate Professor and is a lecturer and an academic researcher. \r\nHer expertise areas are dental caries, cancer, dental fear and anxiety, gag reflex in dentistry, oral medicine, and dentomaxillofacial radiology.",institutionString:"Gazi University",institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"256417",title:"Associate Prof.",name:"Sanaz",middleName:null,surname:"Sadry",slug:"sanaz-sadry",fullName:"Sanaz Sadry",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256417/images/8106_n.jpg",biography:null,institutionString:null,institution:null},{id:"272237",title:"Dr.",name:"Pinar",middleName:"Kiymet",surname:"Karataban",slug:"pinar-karataban",fullName:"Pinar Karataban",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/272237/images/8911_n.png",biography:"Assist.Prof.Dr.Pınar Kıymet Karataban, DDS PhD \n\nDr.Pınar Kıymet Karataban was born in Istanbul in 1975. After her graduation from Marmara University Faculty of Dentistry in 1998 she started her PhD in Paediatric Dentistry focused on children with special needs; mainly children with Cerebral Palsy. She finished her pHD thesis entitled \\'Investigation of occlusion via cast analysis and evaluation of dental caries prevalance, periodontal status and muscle dysfunctions in children with cerebral palsy” in 2008. She got her Assist. Proffessor degree in Istanbul Aydın University Paediatric Dentistry Department in 2015-2018. ın 2019 she started her new career in Bahcesehir University, Istanbul as Head of Department of Pediatric Dentistry. In 2020 she was accepted to BAU International University, Batumi as Professor of Pediatric Dentistry. She’s a lecturer in the same university meanwhile working part-time in private practice in Ege Dental Studio (https://www.egedisklinigi.com/) a multidisciplinary dental clinic in Istanbul. Her main interests are paleodontology, ancient and contemporary dentistry, oral microbiology, cerebral palsy and special care dentistry. She has national and international publications, scientific reports and is a member of IAPO (International Association for Paleodontology), IADH (International Association of Disability and Oral Health) and EAPD (European Association of Pediatric Dentistry).",institutionString:null,institution:null},{id:"202198",title:"Dr.",name:"Buket",middleName:null,surname:"Aybar",slug:"buket-aybar",fullName:"Buket Aybar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202198/images/6955_n.jpg",biography:"Buket Aybar, DDS, PhD, was born in 1971. She graduated from Istanbul University, Faculty of Dentistry, in 1992 and completed her PhD degree on Oral and Maxillofacial Surgery in Istanbul University in 1997.\nDr. Aybar is currently a full-time professor in Istanbul University, Faculty of Dentistry Department of Oral and Maxillofacial Surgery. She has teaching responsibilities in graduate and postgraduate programs. Her clinical practice includes mainly dentoalveolar surgery.\nHer topics of interest are biomaterials science and cell culture studies. She has many articles in international and national scientific journals and chapters in books; she also has participated in several scientific projects supported by Istanbul University Research fund.",institutionString:null,institution:null},{id:"260116",title:"Dr.",name:"Mehmet",middleName:null,surname:"Yaltirik",slug:"mehmet-yaltirik",fullName:"Mehmet Yaltirik",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/260116/images/7413_n.jpg",biography:"Birth Date 25.09.1965\r\nBirth Place Adana- Turkey\r\nSex Male\r\nMarrial Status Bachelor\r\nDriving License Acquired\r\nMother Tongue Turkish\r\n\r\nAddress:\r\nWork:University of Istanbul,Faculty of Dentistry, Department of Oral Surgery and Oral Medicine 34093 Capa,Istanbul- TURKIYE",institutionString:null,institution:null},{id:"172009",title:"Dr.",name:"Fatma Deniz",middleName:null,surname:"Uzuner",slug:"fatma-deniz-uzuner",fullName:"Fatma Deniz Uzuner",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/172009/images/7122_n.jpg",biography:"Dr. Deniz Uzuner was born in 1969 in Kocaeli-TURKEY. After graduating from TED Ankara College in 1986, she attended the Hacettepe University, Faculty of Dentistry in Ankara. \nIn 1993 she attended the Gazi University, Faculty of Dentistry, Department of Orthodontics for her PhD education. After finishing the PhD education, she worked as orthodontist in Ankara Dental Hospital under the Turkish Government, Ministry of Health and in a special Orthodontic Clinic till 2011. Between 2011 and 2016, Dr. Deniz Uzuner worked as a specialist in the Department of Orthodontics, Faculty of Dentistry, Gazi University in Ankara/Turkey. In 2016, she was appointed associate professor. Dr. Deniz Uzuner has authored 23 Journal Papers, 3 Book Chapters and has had 39 oral/poster presentations. She is a member of the Turkish Orthodontic Society. Her knowledge of English is at an advanced level.",institutionString:null,institution:null},{id:"332914",title:"Dr.",name:"Muhammad Saad",middleName:null,surname:"Shaikh",slug:"muhammad-saad-shaikh",fullName:"Muhammad Saad Shaikh",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Jinnah Sindh Medical University",country:{name:"Pakistan"}}},{id:"315775",title:"Dr.",name:"Feng",middleName:null,surname:"Luo",slug:"feng-luo",fullName:"Feng Luo",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Sichuan University",country:{name:"China"}}},{id:"423519",title:"Dr.",name:"Sizakele",middleName:null,surname:"Ngwenya",slug:"sizakele-ngwenya",fullName:"Sizakele Ngwenya",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419270",title:"Dr.",name:"Ann",middleName:null,surname:"Chianchitlert",slug:"ann-chianchitlert",fullName:"Ann Chianchitlert",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419271",title:"Dr.",name:"Diane",middleName:null,surname:"Selvido",slug:"diane-selvido",fullName:"Diane Selvido",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419272",title:"Dr.",name:"Irin",middleName:null,surname:"Sirisoontorn",slug:"irin-sirisoontorn",fullName:"Irin Sirisoontorn",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"355660",title:"Dr.",name:"Anitha",middleName:null,surname:"Mani",slug:"anitha-mani",fullName:"Anitha Mani",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"355612",title:"Dr.",name:"Janani",middleName:null,surname:"Karthikeyan",slug:"janani-karthikeyan",fullName:"Janani Karthikeyan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"334400",title:"Dr.",name:"Suvetha",middleName:null,surname:"Siva",slug:"suvetha-siva",fullName:"Suvetha Siva",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}}]}},subseries:{item:{id:"1",type:"subseries",title:"Oral Health",keywords:"Oral health, Dental care, Diagnosis, Diagnostic imaging, Early diagnosis, Oral cancer, Conservative treatment, Epidemiology, Comprehensive dental care, Complementary therapies, Holistic health",scope:"
\r\n This topic aims to provide a comprehensive overview of the latest trends in Oral Health based on recent scientific evidence. Subjects will include an overview of oral diseases and infections, systemic diseases affecting the oral cavity, prevention, diagnosis, treatment, epidemiology, as well as current clinical recommendations for the management of oral, dental, and periodontal diseases.
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In recent years, the application of chemistry to biological molecules has gained significant interest in medicinal and pharmacological studies. This topic will be devoted to understanding the interplay between biomolecules and chemical compounds, their structure and function, and their potential applications in related fields. Being a part of the biochemistry discipline, the ideas and concepts that have emerged from Chemical Biology have affected other related areas. This topic will closely deal with all emerging trends in this discipline.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/15.jpg",keywords:"Phenolic Compounds, Essential Oils, Modification of Biomolecules, Glycobiology, Combinatorial Chemistry, Therapeutic peptides, Enzyme Inhibitors"},{id:"17",title:"Metabolism",scope:"Metabolism is frequently defined in biochemistry textbooks as the overall process that allows living systems to acquire and use the free energy they need for their vital functions or the chemical processes that occur within a living organism to maintain life. Behind these definitions are hidden all the aspects of normal and pathological functioning of all processes that the topic ‘Metabolism’ will cover within the Biochemistry Series. Thus all studies on metabolism will be considered for publication.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/17.jpg",keywords:"Biomolecules Metabolism, Energy Metabolism, Metabolic Pathways, Key Metabolic Enzymes, Metabolic Adaptation"},{id:"18",title:"Proteomics",scope:"With the recognition that the human genome cannot provide answers to the etiology of a disorder, changes in the proteins expressed by a genome became a focus in research. Thus proteomics, an area of research that detects all protein forms expressed in an organism, including splice isoforms and post-translational modifications, is more suitable than genomics for a comprehensive understanding of the biochemical processes that govern life. The most common proteomics applications are currently in the clinical field for the identification, in a variety of biological matrices, of biomarkers for diagnosis and therapeutic intervention of disorders. From the comparison of proteomic profiles of control and disease or different physiological states, which may emerge, changes in protein expression can provide new insights into the roles played by some proteins in human pathologies. Understanding how proteins function and interact with each other is another goal of proteomics that makes this approach even more intriguing. Specialized technology and expertise are required to assess the proteome of any biological sample. Currently, proteomics relies mainly on mass spectrometry (MS) combined with electrophoretic (1 or 2-DE-MS) and/or chromatographic techniques (LC-MS/MS). MS is an excellent tool that has gained popularity in proteomics because of its ability to gather a complex body of information such as cataloging protein expression, identifying protein modification sites, and defining protein interactions. The Proteomics topic aims to attract contributions on all aspects of MS-based proteomics that, by pushing the boundaries of MS capabilities, may address biological problems that have not been resolved yet.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/18.jpg",keywords:"Mono- and Two-Dimensional Gel Electrophoresis (1-and 2-DE), Liquid Chromatography (LC), Mass Spectrometry/Tandem Mass Spectrometry (MS; MS/MS), Proteins"}],annualVolumeBook:{},thematicCollection:[],selectedSeries:{title:"Biochemistry",id:"11"},selectedSubseries:null},seriesLanding:{item:null},libraryRecommendation:{success:null,errors:{},institutions:[]},route:{name:"chapter.detail",path:"/chapters/9657",hash:"",query:{},params:{id:"9657"},fullPath:"/chapters/9657",meta:{},from:{name:null,path:"/",hash:"",query:{},params:{},fullPath:"/",meta:{}}}},function(){var t;(t=document.currentScript||document.scripts[document.scripts.length-1]).parentNode.removeChild(t)}()