\r\n\tIn conclusion, this book is intended for Engineers for research in the domains of speech signals and ECG denoising and also in the domain of image denoising. Many mathematical tools can be used for speech enhancement, ECG Denoising, and Image Denoising. Among those tools, we can mention wavelets, Empirical Mode Decomposition, Total Variation Denoising, Non-Local Means (NLMS), Kalman Filtering, Wiener Filtering, Deep Learning, etc.
",isbn:"978-1-83768-030-6",printIsbn:"978-1-83768-029-0",pdfIsbn:"978-1-83768-031-3",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,hash:"9885534183ae520bcc63a91d4d083390",bookSignature:"Dr. Mourad Talbi",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11943.jpg",keywords:"Speech Enhancement, Thresholding, Signal to Noise Ratio, Wavelets, ECG Denoising, Empirical Mode Decomposition, Total Variation Denoising, Image Denoising, SNR, Non-local Means (NLMS), Kalman Filtering, Wiener Filtering",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 11th 2022",dateEndSecondStepPublish:"June 8th 2022",dateEndThirdStepPublish:"August 7th 2022",dateEndFourthStepPublish:"October 26th 2022",dateEndFifthStepPublish:"December 25th 2022",remainingDaysToSecondStep:"21 days",secondStepPassed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"Assistant Professor Mourad Talbi obtained his Ph.D. in Electronics at the Faculty of Sciences of Tunis, Tunisia. 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In particular, the accumulation of floating debris is a serious problem in the Mediterranean whose hydrodynamics comprise an inflow of surface water from the Atlantic. Several researchers argue that plastic litter is owed in large quantities to traveling vessels [1, 2]. It was argued that about 90% of consumer goods worldwide are moved through an international fleet of cargo ships and most of these goods are contained or wrapped in plastic. Hundreds of containers tip over in rough seas every year and eventually sink and break open, releasing their contents in the ocean. There are several cases of tipped containers that were abandoned on the seafloor. Off the coast of Monterrey Bay, California, over twenty-four containers were lost in a February 2004 storm, one of which is still resting on the seafloor at a depth of about 1500 meters [3].
Significant plastic loads originate from the shore from various sources such as solid waste dumps, trash conveyed from dry land to shore through rivers, and ocean currents carrying plastic debris from shore to shore. There have been several documentaries by environmental activists showing that rivers are a significant source of solid waste, in particular plastic, that make it into the marine environment [4]. There is no evidence yet that the Mediterranean contains a permanent trash island, but there are signs of litter accumulation. Possible retention areas can be identified by analyzing historical data to compute the probability of debris particles circulating into subbasins. The prediction of the location of floating trash islands becomes feasible once accumulation rates are quantified. Climatological reconstructions were performed using empirical data to study the evolution of litter concentration. Floating litter has a general tendency to concentrate in the southern portion of the Mediterranean [5]. A long-term accumulation of litter seems to be forming in the southern and southeastern Levantine basin.
Based on data collected and processed by Zambianchi et al., note how the near-surface drifters were used to track currents starting from a dispersed status (red dots) and ending along coasts on the East Mediterranean including Lebanon and North African countries [5]. Besides the Adriatic Sea portion, and focusing on the Mediterranean, a large percentage of the ending-points concentrate on the East Mediterranean coast, including Lebanon for both summer and winter seasons (Figure 1). As such, the author recommends that special attention be placed on the Levantine area to estimate existing, and forecast potential, plastic debris accumulation.
Drifter starting and ending points in total; starting and ending points in summer and winter. Source: Zambianchi et al. [
Several sources of secondary data in the literature provide quantitative estimates about plastic pollution at large. In Europe and the United States, significant progress was made in data collection and availability in relation to weight, volume, and movement of plastics from their original points on dry land to the oceans. However, such data is not as available or reliable in Lebanon and several countries around the Mediterranean. In subsequent parts of this chapter, it is recommended that fieldwork, surveys, and quantification techniques be developed over the next few years to build a database in support of decision-making and policy design.
It is estimated from various studies that about 8 million metric tons of plastic waste per year travels to oceans worldwide and this figure is expected to increase. Studies in the U.S. and Australia present computational models that analyze data from 192 countries with coastlines. Starting from a total amount of solid waste generated as household refuse per capita, an assumption is made about its percentage of plastics. Another assumption is made about the proportion that will reach the oceans to estimate these amounts over the next decade on the basis of census data and population growth [6].
Results from different sources show corroboration that marine plastic debris, which originates inland and travel the most through rivers. A simplified infographic in Figure 2 shows residential, commercial, and industrial conglomerates mismanaging plastic waste and discarding it in rivers, which in turn carry it to the oceans, terming the process as “plastic load.” A study of 57 rivers investigated the concentration of plastic bags, bottles, fibers, and beads, and used the river flow to compute the total weight of plastic transported by the river to the ocean. Plastic litter per day per person was computed for each river and compared with littering onshore. Rivers were proven to be substantial conveyors of plastic debris into the marine environment. Schmidt et al., compiled global data on plastics in the water in rivers of a wide range of flows, including macroplastics (particles >5 mm) and microplastics (particles <5 mm). They identified mismanaged plastic waste (MMPW) discharged over river catchments basins, transported along the main river artery and into the sea [7]. They concluded that the mathematical relationship is not linear. Major rivers surrounded by densely populated areas generate a higher fraction of MMPW. Further, they concluded that 88–95% of the global plastic river load is conveyed through the ten top-ranked rivers, with an estimated range between 410 tons and 4 million tons per year [7]. This large disparity is owed to uncertainty in data. In fact, such a model requires additional parameters related to seasonality, climate change, hydrological factors, and local or regional precipitation [8]. There has been a misconception related to river loads in the articles attributed to ten rivers that these ten rivers take 90% of total plastic pollution in the oceans. It should be clarified that the 90% estimate of the top ten river load is a percentage of total river load and not total ocean pollution.
Rivers exert “plastic loads” from common use and mismanaged plastic waste that make their way to the oceans Schmidt et al. [
Most studies on river plastic loads address the largest ones across continents, while little attention was given to smaller rivers in countries around the Mediterranean. Nevertheless, it is useful to provide some context by comparing the Nile, which is the largest river with an estuary on the Mediterranean, with other major rivers worldwide. The top 10 rivers worldwide that were listed among polluters include the Nile and Niger, both of which are African rivers, and the others are Asian rivers. Note that the Yangtze alone was estimated to carry about 2.75 tons of plastic yearly into the sea [9]. Alarming documentaries have been shared on social media showing the Yangtze, among other rivers exhibiting the same problem, covered in opaque blotches of plastic waste (Figure 3).
The Yangtze River with workers collecting floating garbage (source:
The Nile River flows over 6600 kilometers and runs through (or along the border of) South Sudan, Sudan, Ethiopia, Uganda, Kenya, Congo, Rwanda, Tanzania, Burundi, and Egypt into the Mediterranean Sea making it the longest river in the world. The river has been critical in the development of ancient Egypt as it provided for irrigation, transportation, and fishing among other features. In recent years, over 90% of the Egyptian population lives only within a few kilometers of the Nile riverbanks using ferries, water taxis, and private boats for commute, leisure, or tourism. Today, the Nile is ranked among the top ten polluting rivers (Figure 4).
Top 10 river polluters, among them the Nile flowing into the Mediterranean. Source: “Export of Plastic Debris by Rivers into the Sea,” by Christian Schmidt et al. [
Research in the literature shows that the Mediterranean Sea suffers from high concentrations of floating plastic. This is an indicator that a trend is developing akin to the accumulation zones in the five subtropical ocean gyres. According to a study in the year 2015, an average density of plastic was found to be about 1 item per 4 m2. It also showed that the frequency of occurrence is 100%, in the sense that all sampled sites showed an occurrence (presence) of plastic debris in the water. Plastic items found were in the range of millimeters. Large plastic items were also found to be in higher proportions than those documented in the oceanic gyres [10]. Most likely, this is due to the fact that sources of pollution around the Mediterranean are closer. Floating plastic in the Mediterranean Sea was estimated to be between 1000 and 3000 tons. Schmidt et al. found the Nile to be the fifth river among the top ten polluting rivers worldwide [7]. Figure 4 shows the contributions of the top ten polluting rivers with the estimated quantities of plastic transport in hundred thousands of metric tons. Ten countries sharing the benefits of the Nile bordering or running through them have a moral obligation toward each other to curb plastic waste at the source. They also have an obligation toward countries around the Mediterranean that are recipients, as well as contributors, of plastic waste on their shores. Therefore, the problem requires collaboration at a regional level.
In terms of solid and plastic waste, the case of Lebanon carries even more uncertainty due to challenges in data availability, collection, analysis, and estimation. Lebanese rivers convey large amounts of solid waste and plastic debris into the Mediterranean, but disproportionately large amounts are due to direct dumps of solid waste and landfills on its coast. Landfills along the Lebanese shore are the source of sea pollution in the direct vicinity of the beaches. This makes the estimation of river plastic load even more complicated to relate to the river’s water discharge rate. A previous estimate by the author used a model that combined population density around major Lebanese rivers, industrial activity, and average yearly discharge. The estimation model at the time focused on percentages rather than absolute numerical values with the objective of directing efforts for awareness and advocacy within the context of general solid waste inclusive of, but not limited to, plastics [8].
It would be necessary to pursue these estimates by collecting primary data from landfills, rivers, and direct dumps to update a geographic mapping focused on plastics transported through rivers into the sea. If we consider river flows exclusively, then we would find that Nahr el Kabir, Ostuene, Araqa, El Bared, Abou Ali, and Al Jaouz would constitute about 24% of total Lebanese river discharge. Nahr Ibrahim would constitute about 13% and Nahr el Kalb 6% of total Lebanese river discharge. However, the presence of landfills and industrial activity yield different numerical values. For example, even though Nahr Beirut contributes about 3% of Lebanese river discharge, the presence of landfills, population density, and industrial activity tripled the percentage in that particular zone yielding 10% and 11% between Narh el Kalb and Nahr Beirut (Figure 5).
Percentage estimates of solid waste distribution along the Lebanese coast. (Source: Chalhoub [
The question remains: Is solid waste at large a good indicator or proxy for plastic waste? By most standards, the proxy between the two indicators is close enough to proceed with a subsequent phase of primary data collection, documentation, analysis, and awareness building.
Several studies were performed about solid waste in Lebanon but there is a need to develop detailed observations and recommendations specific to plastics. According to a Country Report on Solid Waste Management in Lebanon, 1.57 million tons of municipal solid waste (MSW) was generated in Lebanon in 2009 [11]. Knowing the challenge of reaching an accurate census with the refugee situation in Lebanon, and using a simplified estimated population, the average waste generation per day per capita is estimated at 1.1 kg/day/capita in urban areas and 0.7 kg/day/capita in rural areas, with about 50% of the waste being organic. Nevertheless, plastics constitute 13% while paper, pulp products, and cardboard constitute 17%. It is expected that, in absence of focused public policies, waste generation will continue to rise annually by 1.65% [11]. The same study in 2010 reports that 30% of MSW is dumped in the open, 53% is land-filled, while only 9% is composted and 8% recycled. In Lebanon, the largest landfill is in Naameh, south of Beirut, which was closed following a public uproar [12]. Beirut generates the highest proportions of MSW in the country due to the fact that about 50% of the population lives in the capital and its direct suburbs, in addition to being a tourist attraction with a concentration of hotels and restaurants.
The Syrian refugee crisis affected demographics in Lebanon. By May 2014, Lebanon received over 35% of the region’s Syrian refugees with an estimate of an additional 1000 tons/day of solid waste. Under such considerations, there are projections that by 2025, MSW would surpass 2.4 million tons [11, 13]. Studies showed that 37% of MSW in Lebanon could be recycled or reused, except for organic waste which can be composted into other products such as fertilizers [14]. Since there has not been a clear national strategy for solid waste management, MSW remains a critical problem with several environmental, economic, and public health impacts. Even though MSW in large Lebanese cities such as Tripoli, Beirut, Zahle, and Sidon is collected through private companies, the Lebanese end-user should adopt a preventive approach by reducing waste generation including plastics, and by looking for alternatives [8].
Lebanese plastic companies serve local buyers but have well-established export markets. In 2014, Lebanon imported raw materials for the manufacturing of plastic semifinished and finished products in the value of $695 Mn, while it exported a total value of $131 Mn in raw materials and finished products [15]. Among the top seven Lebanese plastics manufacturers, there are those who produce food packaging, followed by containers, bottles, then piping products, furniture, agricultural materials, hospital consumables, bags, and other industrial materials. While only 10% of discarded plastics are recycled, the remaining quantities go to landfills and the sea. Three sectors, shipping, fishing, and tourism are briefly addressed to illustrate their socio-economic impact and highlight the role that they could fulfill in curbing marine plastic pollution.
Historically, Lebanon is known for its shipping and transit activities. Despite the civil war in the 1970s and the current economic meltdown, maritime transport still plays an important role in the Lebanese economy. There are dozens of small ports all along Lebanon’s coast used for limited activities and primarily fishing, in addition to twelve marine centers for leisure. But the main commercial ports in Lebanon where the bulk of maritime activities reside are located in Beirut, Tripoli, and Saida [16, 17].
Stretched on a 1.18 km2 plot at the foothill of Ashrafieh, the Port of Beirut constitutes the main port of trade in Lebanon transiting products to Iraq, Jordan, the Gulf States, Syria, among other countries. The recent August 4, 2020 explosion crippled its activities but despite the extensive damage that it has endured, it started to limp back into a running operation in the following few months [18]. The port of Tripoli is ranked as the second commercial port in Lebanon. It covers a larger plot of about 2.9 km2, but it is still less technically developed than the one in Beirut.
Secondary data indicates that, over the time span between 2014 and 2018, Tripoli docked between 500 and 870 ships per year while Beirut docked between 1600 and 2000 ships. Over the same time period, Beirut Port processed an average of 8 million tons of merchandise per year tonnage while Tripoli processed between 1.3 and 1.9 tons. As for Sidon Port (Saida), it is of a different character; more of a celebrated historic site, of lesser commercial stature, used mainly for small boats and about 190 ships per year [18]. Shipping is both a contributor to plastic pollution as well as a sector that suffers from plastics at docking facilities and potential hindrance at sea.
Fishing in Lebanon has primarily three segments; sea fishing, fish farms, and imports. We focus on sea fishing as it is the one relevant to plastic pollution in Lebanese waters and on its shores. Lebanese fishing activities are considered as small businesses consisting of a single person each, with a crew of two or three helpers, hence closer to artisanal than industrial. With Mediterranean waters housing off the coast of Lebanon over 1700 marine fauna species, with over 82 fish species sold on the local market. Commonly used tools include small mesh nets used to fish near the seafloor. “Ghost fishing” is a term used to indicate lost nets. These nets are made of plastic wire mesh and cause various sorts of hindrance and death of untargeted marine organisms. Most fishing is performed at depths between 40 m and 65 m, and about 92% of fishermen operate at about a nine-kilometer radius from their base harbors. Ghost fishing is one of the concerns related to plastic pollution in marine waters.
The Ministry of Agriculture comprises a Fisheries and Wildlife Department that publishes data sporadically. There has been an increase in sea fishing activity between 1995 and 2005. In 1994, small-operation fishermen totaled about 4100 with a fleet of approximately 2050, while in 2005, the number was between 4500 and 9600 commensurate with the active seasons most of whom were Lebanese (89%).
Fishermen are considered among the poor population segment because their annual income is less than the minimum wage annual income population. Secondary data indicates that the annual average range between $800 and $2100 while the minimum wage corresponded (in 1999) to $5400 per year [18, 19]. Research by Kanbar shows a distinctive difference in income between those who own their boats, averaging $7400 per year, and those who lease or are loosely employed by boat owners, averaging $ 3000 [17]. Fishing contributes to plastic pollution and simultaneously suffers from plastic litter, which reduces productivity, fish reproduction (quantity), and fish quality. Further, fishing boats are also hindered at sea.
Lebanon has always depended on tourism in its economy. There are various dimensions through which tourism contributes to economic growth. Earnings from tourism provide foreign currencies, which in turn support imports. Further, destination marketing enhances healthy competition among local service providers and hospitality establishments [20, 21, 22]. Another indirect effect of tourism is that it creates demand for skilled human resources and new infrastructure, which requires investment. Tourism increases demand for products and services in construction, manufacturing, transportation and rental vehicles, and financial services such as insurance and banking [23, 24].
Tourism in Lebanon showed an overall upward trend between 1961 and 2011. Between 1996 and 2010, arrivals of tourists in Lebanon increased by a factor of 2.5. The tourism sector is sensitive to security and political developments, which explains fluctuations including a drop down from 7.8 million arrivals in 2004 to 5.9 million in 2005. In 2011, when the civil war broke out in Syria, Lebanon saw another drop in total arrivals from 9 million in early 2011 to 5.8 in 2016 [25].
In 2018, the total contribution of tourism and travel to Lebanon’s GDP was 19%, down from 27% in 2010 and 18% in 2017 [26]. That was the result of several events including travel bans, the refugee situation, regional and internal political tensions, and most of all local mismanagement in the public sector. Most of the inbound tourists are Lebanese living overseas or Arabs, with the percentage of Arab tourists decreasing from 59% in 2013 to around 41% in 2018. The tourism sector is affected by solid waste crises, among others, as was seen in the last two decades [18, 26, 27].
Tourism contributes directly and indirectly to job creation. Despite the drop in arrivals, there were about 395 thousand jobs in 2018, up from 365 thousand in 2017, while overall, the percentage of total employment decreased to 18% in 2017, down from 24% in 2010 [26]. Besides hotel services, travel agents, leisure, and such jobs are directly related to tourism, there are indirect jobs such as cleaning, food and beverage, suppliers to companies providing hospitality services, local tours activities, and the like. Therefore, the percentage affected by the tourism sector would be in the range of 33%, which is significant.
Plastic pollution in rivers, on the beaches, and in the streets have a drastic effect on touristic, hence economic activity. For instance, in 2017, hotels officially registered in the Syndicate of Hotel Owners reached 408 with a total of 21,804 rooms most of which are located on the coast. Secondary data from 2008 indicate that 68 beach resorts occupy 7.5% of the coastal square footage. By 2017, their number increased to 200, of which 65% are located between Byblos and Beirut, over a 38-kilometer distance along the Mediterranean coast [28]. Therefore, the way solid and plastic waste are managed by those resorts has a large impact on marine pollution.
The rush in the mushrooming of beach resorts caused an overdevelopment on the shore including several environmental breaches bypassing environmental impact assessments and permitting processes. This phenomenon has a double effect; excessive waste generation as well an effect on environmental quality. While plastic pollution has an obviously negative visual effect for tourists, microplastics have a health effect as it is transmitted to humans from fish and seafood. A study by the author on tourism entities operating on the coast between Adonis City and Tabarja shows that those establishments generate six-fold the waste in summer compared to winter. Tourism contributes to plastic waste but it is also negatively impacted by plastic pollution which spoils the beauty of the shores, the attractiveness of leisure sea trips, and increases risks of digestive diseases in hospitality establishments due to microplastics in fish and seafood.
The above sections show that the economic impacts of marine plastic pollution on shipping, fishing, and tourism are disconcerting. Cleanup costs, losses in fishing, ghost fishing, boat damage, economic losses in tourism, and ship hindrance are all effects that require prevention of plastic litter rather than remediation. There should be a joint effort between the private and public sectors to collect reliable data, on a continuous basis, and categorize plastic waste by description (type) and quantities. Such data would help develop a more accurate evaluation of how marine litter impacts these three areas in the private sector. Additionally, the more challenging quantification pertains to marine ecosystems and the reduction in marine life reproduction and mobility.
We continue our illustration with the case of Lebanon as it represents a less developed country, located on the Mediterranean, subject to plastic pollution, and faced with public policies and management challenges. Local authorities such as municipalities at a town or city level have been traditionally entrusted with solid waste collection and disposal. In recent decades, nongovernmental organizations intervened and started to provide services, some funded by international agencies and some based on volunteerism. Either way, a significant social cost is incurred to remove marine litter. For beach cleanup projects, which became in vogue in the last ten years, there is a cost of mobilization, time away from the job (for volunteers), and cost of labor when done as paid professional services. Cleanup cost includes collection, loading, transportation, disposal, and when applicable, recycling. Therefore, the economic opportunity cost of cleaning depends on the line of business that is being forfeited within the local economy.
There have been attempts to quantify such activities in highly developed countries, whereby such studies were performed by the public sector. In the United Kingdom, port authorities spent Euros 2.3 million on debris removal from harbors, while the cost of removing plastic bags on beaches was estimated Euros 340,000/year. Other examples from Belgium and the Netherlands show that about Euros 10 million/year were spent to remove solid waste off beaches [29, 30].
Various studies showed that beach cleanliness ranks as a top priority for tourists and visitors [31]. An analysis of litter impact on beach visitors in the Cape Peninsula in South Africa found that 97% of visitors would not be allowed to a beach with 10/m2 or more of large litter items. This indicates that marine litter threatens the image of a country like Lebanon and ultimately leads to a decline in its tourism, which corresponds to 20% of its GDP as seen in previous sections. Another area of public-private cooperation is that, according to UNEP, tourism not only drops from the marine litter but also contributes to it [32].
Several activities led by NGOs and advocacy groups, while noteworthy in terms of community service in fighting plastic pollution, are considered by the author as merely reactive. Granted that beach cleaning campaigns, distribution of trash containers for plastic collection, and offering trucking services from trash collection points to recycling plants are all necessary remedial activities, they fall short of offering a long-term solution to plastic pollution. The circle of manufacturing, consuming, dumping, collecting, and recycling plastics has its own carbon footprint problems that should be thoroughly evaluated and gradually replaced by preventive strategies.
Private sector establishments in Lebanon, namely the ones that are active in plastics trading and manufacturing have an opportunity to play a critical role in partnering with the public sector in a national and regional effort. New process innovations, manufacturing techniques, and new product development must be implemented in the Lebanese manufacturing sector. To reach such operational achievements, concepts of corporate social responsibility must become an integral part of the companies’ vision and strategy.
There has been a flawed argument that there is tension between industrial activity and job creation on one side, and environmental preservation on the other side. Numerous examples confirm the opposite in that sustainability in strategic planning and environmental consciousness are aligned with long-term results in business development. Private sector entities, plastic traders, producers, and corporate users are increasingly subjected to changes in consumer awareness about plastics as hazardous waste. Therefore, they need to embrace corporate social responsibility to align and anticipate market needs.
Figure 6 summarizes the relationships among various stakeholders and how actions among private sector establishments, public sector entities, and the citizen are interrelated. The local population must be engaged in awareness campaigns, and the manufacturing sector is to be part of advocacy campaigns. Radical changes are required through preventive strategies. Since the Lebanese coast is not only a source of plastic litter but is also a destination, a collaboration at a regional level would serve best the common interest to protect this shared sea [33].
A citizen- and consumer-centered view to a proposed interaction and public-private partnership in fighting plastic pollution.
Among multiple sources of plastic pollution, rivers carry solid waste from inland to the ocean from residential, industrial, and agricultural areas. There has been an increased awareness about the hazards of plastic pollution and threat to human, animal, and vegetation life. While the maritime transport industry was blamed for many decades as the main source of marine plastic pollution, it is found that river load and dumps on the shores are major contributors. The problem is exacerbated by movements from shore to shore by waves and currents. Our overview about river solid waste loads calls for urgent actions to be taken by the private sector by embracing corporate social responsibility. The Mediterranean, which is particularly fragile because of its enclosed geography, suffers from industries in several countries around the basin that generate toxic or solid waste disposed of in rivers and municipal sewage networks. A practical application was presented using data from Lebanon, located on the easternmost shore of the Mediterranean, and whose economy depends by and large on service sectors including transit from its harbors to inland countries in the region. The private sector is expected and recommended to play a leading role in curbing plastic pollution by partnering with the public sector. Companies are encouraged to be proactive in the process and product innovation, and the public sector is recommended to stimulate such preventive corporate strategies through the enactment of thoughtful and forward-looking public policies. While remedial actions such as cleanup and recycling are necessary, the solution resides in migration toward green products and the development of bio-substitutes to plastics. A concerted effort to fight plastic pollution onshore and in the marine environment is required through partnerships between companies and local governments.
A signal is a function that conveys information about the behavior or attributes of some phenomenon [1]. On the other hand, information can be anything. A waveform can have multiple overlapping information in the same space–time. The signal in a waveform is subjective, it can be color for one and shape for the other. In electrophysiology, waveform under inspection can be separated into two as the signal of interest and noise. The signal can be electrocardiography (ECG), Electroencephalogram (EEG), or any other physiological signal, noise is any unwanted wave source ınterfering with the signal. If we consider EEG as the signal, it is recorded from the scalp by electrodes and consists of the overall electrical activities of neural populations and a contribution of glial cells [2]. EEG has a wide range of use in both clinical practice and engineering applications in medicine, particularly neurology, sleep, and epilepsy research.
The EEG recording environment and subject related electrical activities during recording deteriorate the signal quality. Artifacts are undesired signals that may introduce changes in the measurements and affect the signal of interest [3]. EEG can be contaminated in frequency or time domain by artifacts that are resulted from internal sources of physiologic activities and movement of the subject and/or external sources of environmental interferences, equipment, movement of electrodes and cables [4]. Artifact types and sources are listed in the Table 1. External artifacts can be prevented by proper shielding, grounding cables, isolating and moving cables away from recording sites since they act as antennas during operation. On the other hand, internal or physiological artifacts are challenging for researchers because of their inclusion of signal or resemblance to the signals. The most important artifacts in a typical EEG recording are ocular electro-oculogram (EOG) artifacts and muscular (EMG) artifacts.
Artifact | Type | Source |
---|---|---|
Eye blink | Ocular | Internal/Physiological |
Eye movement | Ocular | Internal/Physiological |
REM Sleep | Ocular | Internal/Physiological |
Scalp contractions | Muscle | Internal/Physiological |
Glossokinetic artifact | Muscle | Internal/Physiological |
Chewing | Muscle | Internal/Physiological |
Talking | Muscle | Internal/Physiological |
EKG | Cardiac | Internal/Physiological |
Swallowing | Muscle | Internal/Physiological |
Respiration | Respiratory | Internal/Physiological |
Galvanic Skin Response | Skin | Internal/Physiological |
Sweating | Skin | Internal/Physiological |
Electrode movement | Instrumental | External/Extra-physiological |
Electrode Impedence Imbalance | Instrumental | External/Extra-physiological |
Cable movement | Instrumental | External/Extra-physiological |
Electromagnetic coupling | Electromagnetic | External/Extra-physiological |
Powerline | Electrical | External/Extra-physiological |
Head movement | Movement | External/Extra-physiological |
Body movement | Movement | External/Extra-physiological |
Limbs movement | Movement | External/Extra-physiological |
Electrical potentials due to eye opening/closure, blinks, eyelid flutter and eye movements propagate over the scalp and produce hostile EOG artifacts in the recorded EEG. Eye movements are major sources of contamination of EEG. The origin of this contamination is disputable. Cornea-retinal dipole movement, retinal dipole movement and eyelid movement are the three main proposed causes of the eye movement related voltage potential [6]. The direction of eye movements affects the shape of the EOG waveform while a square-like EOG wave is produced by vertical eye movements and blinks which leads to a spike-shaped waveform [7]. Blinks which are attributable to the eyelid moving over the cornea, occurring at intervals of 1-10s, generate a characteristic brief potential of between 0.2 s and 0.4 s duration due to eyelid movement over cornea [8, 9]. The blinking artifact generally has an amplitude much larger than that of the background EEG [6]. It is advantageous to have a reference EOG channel during EEG recording for the cancellation of ocular artifact from EEG activity [3].
Electrical activity on the body surface due to the contracting muscles are recorded via Electromyogram (EMG) [3]. Since independent myogenic activities of head, face and neck muscles are conducted through the entire scalp, it can be monitored in the EEG [10, 11]. The amplitude of this type of artifact is dependent on the type of muscle and the degree of tension [3, 12]. The frequency range of EMG activity is wide, being maximal at frequencies higher than 30 Hz [13, 14].
The electrical potential due to cardiac activity can exhibit itself in the EEG as ECG artifacts. Typical high frequency waveforms similar to EKG P-QRS-T shape are characteristics of EKG artifacts in EEG [15].
Head, body and limb movements cause irregular high voltage artifacts. Artifacts can be produced by tremors in patients such as Parkinson disease and movement disorders. Changing patient position into a calm comfortable stable position helps reducing artifacts. Another prevention for respiratory related movement artifacts is to use a towel or a firm material support for the neck. The changes in the impedance or electrical potential between scalp and electrode may cause electrode artifacts. These can result from poor electrode contact, broken lead, electrolyte gel insufficiency. This type of artifact usually exhibits itself in sudden electrode pops. These electrode artifacts can be eliminated by using proper electrolyte gel, checking electrode impedance, changing the broken electrodes, and shifting the electrode position slightly.
A typical EEG recording system is shown in Figure 1. At the heart of a recording setup is the biopotential amplifier. It should have high common mode rejection ratios, however it should not have high gains, this can saturate the signal due to large half-cell potentials at the electrodes. Unequal electrode impedances are major sources of common mode artifacts such as powerline.
EEG recording system and experiment setup.
Environmental artifacts can be eliminated by bringing the electrodes leads closer together, moving the electrodes and subject away from the noise sources, using single isolated earth for the whole setup, and shielding the cables, machines and artifact sources with a metal tape connected to the common earth. Moreover, the environmental conditions should satisfy the following requirements for proper recordings. These can be listed as, quiet atmosphere, comfortable temperature and humidity, controlled proper lighting, using a comfortable bed or chair, and separating the powerline of the EEG system from the other machines in the lab.
Event Related Potentials (ERP) are electrical signals generated in response to internal or external events and they are recorded by EEG [16]. In evoked potentials, each stimulus produces an evoked potential embedded in EEG. However, since the ERP or evoked potential signals are generally subtle in EEG, averaging of many epochs are needed to make them distinguishable. An ensemble averaging method to enhance the ERPs was defined by [17]. This relies on the assumption that by synchronous averaging of each epoch, signal ERP amplitude adds constructively and EEG background noise diminishes destructively.
In ERP and evoked potential research, artifacts contaminate the final ensemble average signal of interest. One method to overcome this adverse effect is to benefit from a weighted averaging [18]. In weighted averaging technique each epoch is weighted inversely with the non-stationary noise maximum amplitude in the epoch. In [19], each trial’s contribution to ensemble average is multiplied by a weight according to its correlation with the rest of the data. This factor is inversely related to its probability of being an artifact. For example, a large amplitude EEG is likely to be an artifact and the contribution factor for the trial involving large amplitudes will be low whereas the factor for a small amplitude EEG is high (Figure 2). Davila and Mobin [20] showed that weighted averaging of auditory EP has higher SNR than conventional ensemble averaging. John et al. [21] studied the effects of such techniques as sample-weighted averaging, noise-weighted averaging, amplitude based artifact rejection, percentage based artifact rejection, and normal averaging on the steady state auditory evoked potentials. It concluded in favor of weighted averaging for better SNR of steady state responses. On the other hand, according to [22], weighted averaging underestimates the ERP signal amplitude. Determination of the optimal weighting factor is not straightforward and this limits the performance of the weighting averaging method. Mühler and Specht [23] developed a method called ‘sorted averaging’. In sorted averaging, epochs are sorted with RMS values from small to large, since noisy artifactual epochs have large RMS values compared to low noise signals. The signal averaging is performed by addition of epochs from the low noise RMS to large RMS sorted order until a maximum peak of SNR2 is obtained [24]. This eliminates the high RMS noisy epochs and yields a better ERP waveform. Compared to weighted averaging, sorted averaging had significantly higher SNR2 [23].
Various EEG artifacts are shown.
Median averaging is another approach to ERP artifact handling and it is based on taking the median points of all the epochs and adding them to form a median average instead of classic mean average [25]. Some advantages of the median averaging are that; it elicits hidden signals more clearly and it is not affected by infrequent large artifacts that much compared to mean averaging [25]. Özdamar and Kalayci [26] supported the advantages of median averaging over the conventional mean averaging in a study on the ABR signals. Median averaging is an efficient way to remove adverse effects of the outliers on the final averaged signal, yet it also removes the valuable data in the outliers causing significant loss of information [27, 28].
Artifact avoidance, artifact rejection, manual rejection, automatic rejection, and artifact removal are the common methods to deal with artifacts [29]. Although it seems a simple solution to cancel EOG and EMG artifacts by instructing subject to avoid blinking or movement, it can result in change of amplitudes in evoked potentials as well as the additional cognitive load [29, 30, 31]. On the other hand, artifact rejection or manual rejection may require a person dedicated to this purpose of eliminating artifacts visually one by one in an EEG. Moreover, the artifact detection by an expert may be subjective, tedious, and time consuming. In addition, it can not be applicable to online removal [3]. However, automatic rejection can automate this artifact rejection procedure but it can eliminate non-artifact signals if not properly tuned. The automatic rejection of artifact containing EEG can depend on artifact amplitude based or EEG segment RMS based artifact detection and rejection. An example of a simple blink artifact removal is depicted in Figure 3. Since blinks have low frequency content compared to EEG, by low pass filtering, EEG can be reduced while blink artifact still remains at a high voltage level. Thus, an amplitude threshold based artifact rejection can be applied. As seen from Figure 3, red traces are the EEG and blue are the low pass filtered EEG signal. While a simple artifact rejection (without low pass filtering) using a threshold of 20 μV will produce false positives (red traces over 20 μV), in the low pass filtered EEG these false positives are prevented.
Low pass filtering based EEG blink rejection. Red is raw EEG, blue is low pass filered EEG with 6th order Butteworth low pass filter at 8 Hz cut off. The detected artifact containing EEG epochs are shown in dashed rectangles.
Usually one or two channels are dedicated to detect EOG artifacts. There are two widely used procedures for EOG artifacts, first EOG rejection where EEG trials with EOG artifacts having VEOG greater than a preset threshold are omitted, and second EOG correction where the effect of eye movement is tried to be removed from EEG [6].
Artifacts can distort the EEG in a way that the electrophysiologists or physicians can be misled in their clinical interpretation [32]. This makes artifact removal critical in the pre-processing phase prior to analysis. There are many methods to remove artifacts such as Artifactual Segment Rejection, Filtering, Wiener filtering, Adaptive Filtering, Time-Frequency Representation, Wavelet Transform, Discrete Wavelet Transform (DWT), Adaptive Noise Cancelation (ANC), Wavelet Packet Transform (WPT), Kalman Filtering, Linear Regression, Blind Source Separation (Principal Component Analysis (PCA), Independent Component Analysis (ICA), Canonical Correlation Analysis (CCA), Minor Components Analysis (MCA)), Source Decomposition, Empirical Mode Decomposition (EMD), Support Vector Machine (SVM), and hybrid methods [3, 4, 29, 33, 34, 35, 36, 37, 38]. A functional dedicated artifact channel which provides complementary aid to identify ECG/EOG is required to remove ocular or cardiac artifacts in the most of the available methods [4].
Regression is a common and well established technique in artifact removal, yet it cannot be used to remove muscle noise or line noise, since these type of artifacts have no reference channels [39]. Having a good regressor (e.g., an EOG) is critical in both time and frequency domain regression methods. It is an inherent weakness that eye movements and EEG signals are bidirectional. When unacceptable amount of data are lost in artifact rejection, delicate artifact removal methods which will preserve the essential EEG signals while removing artifacts are necessary [39]. One of the most important artifacts is EOG. EEG regions infected with EOG can be rejected from overall EEG signal with simplest artifact rejection where these portions are detected by EOG channels, however these regions still carry brain signals in addition to ocular artifacts and total rejection or subtraction of EOG from them results in loss of brain data [40, 41, 42].
Blind Source Separation (BSS) algorithms utilize multiple channels in an unsupervised learning algorithm to extract brain related activity from the ensemble EEG signal which can be assumed a linear superposition of brain signals, noise and artifacts [38]. Three common BSS algorithms are Independent Component Analysis (ICA), Principal Component Analysis (PCA) and Canonical Correlation Analysis (CCA).
ICA, a BSS method, is often used to remove EEG artifacts based on statistical approach of spatial filtering and separation of multiple channel EEG data into spatially fixed and temporally independent components [39, 43, 44]. Since the EEG sources and artifacts are usually of different origins, they can be assumed to be linear summation of each independent components. ICA method finds these statistically independent components and enable us to eliminate artifactual ones from the desired EEG [45]. On the other hand, ICA provides extraction of the eye related signals present in the EOG, and removal of this information or artifact, rather than the complete EOG which still has some brain activity [40], is possible. However, detection and removal of transient artifacts such as head and neck muscle contractions and movement are difficult with ICA [46]. Moreover, adapting ICA as an online method requires high computational power [46]. On the other hand, an advantage of ICA is that it does not rely on a reference channel [39]. However, many artifact removal algorithms are compared in [3], and Revised Aligned-Artifact Average (RAAA) and Second Order Blind Identification (SOBI) and Adaptive Mixture of Independent Component Analyzers (AMICA) are the preferred artifact removal methods for EOG, EMG and ECG artifacts.
PCA uses orthogonal transform of correlated time domain signal into linearly uncorrelated principal components (PCs) [47]. These principal components possess as much as variance of the EEG as possible. Artifact containing PCs can be eliminated if they are uncorrelated with the brain EEG. Application of PCA into ocular artifacts was provided in [48].
CCA is also another method utilized in removing artifacts. In CCA second order statistics are employed, correlation between two multivariate datasets are maximized by canonical variables. CCA offers shorter computational time compared to ICA [38].
Another method is filtering in frequency domain. Usually a high-pass filter starting from 0.5-1 Hz is applied for baseline drift removal. Notch filters are used to remove powerline-noise. Another one, EMG activity of contracting scalp sites can hinder the signals of interest in the EEG recordings during an epileptic seizure [49]. It was possible to remove this high frequency content EMG activity from EEG spectra by filtering out signals over 25 Hz. Adaptive Filters, Wiener Filtering and Bayesian Filters are three filtering methods applied in EEG signal preprocessing. Adaptive Filters are the most commonly used for artifact removal [47]. In Adaptive Filtering a reference channel for artifacts is subtracted from the EEG recursively. This reference is multiplied by a weight factor obtained from the output of the filter by a learning algorithm and this weighted reference is subtracted from the recorded EEG yielding output artifact free EEG changing adaptively [50].
In wavelet transform, many scaled and time shifted wavelets are used to produce coefficients for the particular signal and wavelet type by convolution of the signal and wavelets. These coefficients indicate similarity between the corresponding wavelet and the signal. In artifact removal via wavelet transform, the main idea is that the signal which can be highly correlated with a basis mother wavelet and can be separated from artifacts which might have no correlation to the principal mother wavelet [50]. Some examples of Wavelet Transform in artifact removal are for ocular artifact removal as in [51, 52].
Recently many preprocessing pipelines have been introduced in order to reduce the burden of artifact handling by an expert one by one visual inspection. This laborious task can be fastened by using existing automatized preprocessing methods in order. An efficient pre-processing pipeline not only helps the artifact management time but also provides objective evaluation with predefined criteria compared to highly subjective artifact handling by a human expert. The preprocessing pipelines usually consist of the combination of the following stages; filtering, re-referencing, bad channel identification (and interpolation), bad channel and epoch removal, artifact detection using ICA, artifact correction and removal [53], see Figure 4.
APP artifact management flow diagram from [
Fully Automated Statistical Thresholding for EEG artifact Rejection (FASTER) [54] algorithm is a state of the art method which is available in EEGLAB toolbox [55]. FASTER has filtering, line noise removal, bad channel detection and interpolation, segmentation, and artifact rejection on segments by identifying bad channels, blinks, eye movements and muscular artifacts using combination of statistical thresholding and ICA [56]. It requires an extra EOG channel. The Automatic Pre-processing Pipeline (APP) removes powerline noise, bad channels, eye movements, blinks and muscular artifacts using ICA to identify artifactual components [53], see Figure 4. However, it also requires extra EOG channels. Da Cruz et al. [53] has found that APP performs better than FASTER yielding higher amplitude in ERP study. Another pipeline is Tool for Automated Processing of EEG data (TAPEEG) [57]. It uses automated routines of FASTER and Fieldtrip for artifact identification and performed similar to visually analysis by an expert [58]. TAPEEG handles the resting state EEG data as well. Both FASTER and TAPEEG are based on z- scores and have difficulty in handling outliers, this leads to loss of signal content due to false positive artifact detection and rejections [53]. Another standardized preprocessing method for large EEG datasets, PREP pipeline, handles line noise removal, bad channel detection, and referencing to standardize and normalize the data before processing [58]. It is also available as plug-in in EEGLAB toolbox.
Automagic is a toolbox developed for standardized handling of large growing EEG/ERP datasets by time [56]. The power of Automagic comes from the fact that it exploits many existing pipelines and methods, such as PREP pipeline for bad channel identification and for average referencing, Cleanline [59] to remove power line noise, EOG regression [60], Multiple Artifact Rejection Algorithm (MARA), ICA or robust PCA for artifact correction [61]. MARA is a plug-in available in EEGLAB which automatically identifies artifacts not only ocular or muscular but also any general artifactual source component in ICA [61]. Pedroni et al. [59] showed that combination of a preprocessing pipeline to identify bad channels and MARA method is efficient to remove most of the artifacts.
None of the methods offers a perfect robust and high accurate management of all types of artifacts. In general, they are all limited with the training dataset and fail to achieve high success with new type of artifactual data.
Since EEG is widely used as a clinical tool to monitor or diagnose patients, doctors can be misguided in case of artifacts and EEG can be misinterpreted. For this reason, artifact removal becomes a crucial point for some cases such as epilepsy monitoring in an EEG/fMRI recording room. Today EEG and fMRI are two distinct but closely related and complementary methods. While fMRI provides high spatial resolution for localization of phenomena in the brain, EEG on the other hand results in better temporal resolution [62, 63, 64, 65]. One should be careful about the experiments involving both fMRI and EEG because there are many unwanted electromagnetic sources interfering with EEG. For example, the false identification of spikes are highly possible since residuals of Ballistocardiogram (BCG) artifacts have similar shapes as epileptic spikes [66]. The factors that can lead to differences in the artifact are linked to the subject and experimental setup, [67]. There are imaging artifacts, cardiac related Ballistocardiogram artifacts (BCG), EOG and EMG artifacts in an EEG inside MRI [44]. Static field (B0) and the time-varying fields of radio-frequency excitations and of imaging gradients, generate artifacts in the EEG known as Ballistocardiogram (BCG) and imaging artifacts [44, 68, 69, 70]. The pulse artifact which can be observed in EEGs recorded inside MR scanners easily, is due to a fundamental cause that any movement of electrically conductive muscles in a static magnetic field generates electromagnetic induction and it is proportional to the static field, generally larger at higher field strengths [67, 71]. Pulsations of the scalp arteries are the main cause of this type of BCG artifact [72, 73]. The study of Grouiller et al. [44] compared different imaging artifact removal techniques and various cardiac artifact correction techniques in both simulated EEG data and in real experimental data. They concluded that there is no key for every door, some algorithms work well for some case and others might work well for other cases. Certain algorithms may be preferred depending on the type of data and analysis method [44]. Another algorithm, adaptive Optimal Basis Set (aOBS), automatically eliminates BCG artifacts yet preserving the neural origin signals in EEG [74]. It can be used efficiently for simultaneous fMRI and EEG recordings.
Manual artifact detection is still the most common method for artifact handling for sleep stage classification, however, the long time required and the difficulty to apply it to large datasets poses the main disadvantages [75]. Malafeev et al. [75] compared 12 simple algorithms that are applicable with a single EEG channel for ease of use. It was found that automatic artifact detection in EEG during sleep within large datasets is possible with simple algorithms. Among these, Power thresholding 25–90 Hz (PT25), Power thresholding 45–90 Hz (PT45) and Autoregressive (AR) models had Reciever Operating Characteristic (ROC) areas above 0.95. In addition, online detection is also possible with the majority of these simple algorithms.
Artifact removal in BCI applications are getting more attention. By studies it was shown that artifacts generated by EOG and EMG activities affect the neurological signals utilized in a BCI system [10, 76]. Although there are extensive researches into artifact removal for BCIs and developed efficient methods such as Fully Online and Automated Artifact Removal (FORCe), Lagged Auto-Manual Information Clustering (LAMIC), Fully Automated Statistical Thresholding for EEG artifact Rejection (FASTER) and K-Singular Value Decomposition (K-SVD), the field lacks an effective artifact removal [12, 54, 77, 78, 79, 80, 81, 82]. The surrogate-based artifact removal (SuBAR) technique proposed by Chavez et al. [33] effectively cancels EOG and EMG artifacts from single-channel EEG. Chang et al. [83] proposed a method for detection of eye artifact from single prefrontal channel which is useful for headband-type wearable EEG devices with a few frontal EEG channels. Compared to conventional methods the accuracy of detecting ocular artifact contaminated epochs was significantly better. Daily-life EEG-BCIs are getting popular and artifact removal techniques for these BCIs must have some critical features such as; must be performed outdoor, with portable wearable wireless device, with real EEG signals, compatible with daily life tasks, must have simple electrical montage, must use dry electrodes, must remove complex artifacts, must work only EEG without reference, must work online and must work with single electrode channel. More research into artifact removal other than ocular and cardiac artifacts is necessary especially for those daily-life EEG BCIs [36].
While ICA and PCA are common artifact removal methods, Artifact Subspace Reconstruction (ASR), which is a powerful automated artifact removal method available for both online real-time and offline, can be applied to prevent transient and large artifact [46, 84]. It also does not require additional channel and cleans the data from artifacts.
The number of artifact handling techniques and algorithms are increasing drastically, however the artifact problem is still challenging for many applications. Particularly, the internal or physiologic artifacts are difficult to distinguish and remove. While simple measures such as artifact avoidance and artifact rejection can be utilized in some applications, most of the cases require special methods dedicated to handle artifacts in order to significantly reduce their harmful effects on signal of interest. Due to the varying nature of artifacts a generic method for all sorts of artifacts is still missing. However preprocessing pipelines provides some efficient approaches to this challenge. In future, the progress in machine learning and deep learning based approaches may yield more efficient, accurate and robust artifact removal options. Online artifact removal methods such as ASR must be developed to overcome various artifacts in daily life to be efficient for BCIs.
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His main focus now is to unravel the mechanism of drought and heat stress response in plants to tackle climate change related threats in agriculture.",institutionString:null,institution:{name:"Indian Council of Agricultural Research",country:{name:"India"}}},{id:"4782",title:"Prof.",name:"Bishnu",middleName:"P",surname:"Pal",slug:"bishnu-pal",fullName:"Bishnu Pal",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/4782/images/system/4782.jpg",biography:"Bishnu P. 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Shohel"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},subject:{topic:{id:"300",title:"Genesiology",slug:"genesiology",parent:{id:"25",title:"Veterinary Medicine and Science",slug:"veterinary-medicine-and-science"},numberOfBooks:2,numberOfSeries:0,numberOfAuthorsAndEditors:73,numberOfWosCitations:52,numberOfCrossrefCitations:34,numberOfDimensionsCitations:79,videoUrl:null,fallbackUrl:null,description:null},booksByTopicFilter:{topicId:"300",sort:"-publishedDate",limit:12,offset:0},booksByTopicCollection:[{type:"book",id:"7233",title:"New Insights into Theriogenology",subtitle:null,isOpenForSubmission:!1,hash:"74f4147e3fb214dd050e5edd3aaf53bc",slug:"new-insights-into-theriogenology",bookSignature:"Rita Payan-Carreira",coverURL:"https://cdn.intechopen.com/books/images_new/7233.jpg",editedByType:"Edited by",editors:[{id:"38652",title:"Dr.",name:"Rita",middleName:null,surname:"Payan-Carreira",slug:"rita-payan-carreira",fullName:"Rita Payan-Carreira"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"5105",title:"Insights from Animal Reproduction",subtitle:null,isOpenForSubmission:!1,hash:"25cd16b683d1f098bc304cbbdb3206cd",slug:"insights-from-animal-reproduction",bookSignature:"Rita Payan Carreira",coverURL:"https://cdn.intechopen.com/books/images_new/5105.jpg",editedByType:"Edited by",editors:[{id:"38652",title:"Dr.",name:"Rita",middleName:null,surname:"Payan-Carreira",slug:"rita-payan-carreira",fullName:"Rita Payan-Carreira"}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}],booksByTopicTotal:2,seriesByTopicCollection:[],seriesByTopicTotal:0,mostCitedChapters:[{id:"49736",doi:"10.5772/62053",title:"Chromosome Abnormalities in Domestic Animals as Causes of Disorders of Sex Development or Impaired Fertility",slug:"chromosome-abnormalities-in-domestic-animals-as-causes-of-disorders-of-sex-development-or-impaired-f",totalDownloads:4132,totalCrossrefCites:7,totalDimensionsCites:20,abstract:"Cytogenetic evaluation is an important step in the diagnosis of infertile or sterile animals. Moreover, the analysis of sex chromosomes is crucial for a proper classification of disorders of sex development (DSD). For many years, chromosome studies mainly addressed the livestock species, while recently, increasing interest in such analysis in companion animals is observed. New molecular and cytogenetic tools and techniques have given opportunities for a precise identification of chromosome mutations. Among them, fluorescence in situ hybridization, besides chromosome banding, has become a gold standard. In this chapter, recent advances in the cytogenetic diagnosis of cattle, pigs, horses, dogs and cats are presented.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Izabela Szczerbal and Marek Switonski",authors:[{id:"177030",title:"Prof.",name:"Marek",middleName:null,surname:"Switonski",slug:"marek-switonski",fullName:"Marek Switonski"},{id:"177045",title:"Dr.",name:"Izabela",middleName:null,surname:"Szczerbal",slug:"izabela-szczerbal",fullName:"Izabela Szczerbal"}]},{id:"49857",doi:"10.5772/62207",title:"Germ Cell Determinant Transmission, Segregation, and Function in the Zebrafish Embryo",slug:"germ-cell-determinant-transmission-segregation-and-function-in-the-zebrafish-embryo",totalDownloads:2278,totalCrossrefCites:4,totalDimensionsCites:11,abstract:"Animals specify primordial germ cells (PGCs) in two alternate modes: preformation and epigenesis. Epigenesis relies on signal transduction from the surrounding tissues to instruct a group of cells to acquire PGC identity. Preformation, thought to be the more derived PGC specification mode, is instead based on the maternal inheritance of germ cell-determining factors. We use the zebrafish as a model system, in which PGCs are specified through maternal inheritance of germ plasm, to study this process in vertebrates. In zebrafish, maternally inherited germ plasm ribonucleoparticles (RNPs) have co-opted the cytoskeletal machinery to reach progressive levels of multimerization, resulting in the formation of four large masses of aggregated germ plasm RNPs. At later stages, germ plasm masses continue to use components of the cell division machinery, such as the spindles, centrosomes, and/or subcellular organelles to segregate asymmetrically during cell division and subsequently induce germ cell fate. This chapter discusses the current knowledge of germ cell specification focusing on the zebrafish as a model system. We also provide a comparative analysis of the mechanism for germ plasm RNP segregation in zebrafish versus other known vertebrate systems of germ cell preformation, such as in amphibian and avian models.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Celeste Eno and Francisco Pelegri",authors:[{id:"177209",title:"Prof.",name:"Francisco",middleName:null,surname:"Pelegri",slug:"francisco-pelegri",fullName:"Francisco Pelegri"}]},{id:"62171",doi:"10.5772/intechopen.79106",title:"Intraoviductal Instillation of a Solution as an Effective Route for Manipulating Preimplantation Mammalian Embryos in vivo",slug:"intraoviductal-instillation-of-a-solution-as-an-effective-route-for-manipulating-preimplantation-mam",totalDownloads:1124,totalCrossrefCites:8,totalDimensionsCites:9,abstract:"Preimplantation embryos of mammals are enclosed by a translucent layer called zona pellucida (ZP), which is composed of glycoproteins. ZP is important for protecting against infection by virus and bacteria, and to prevent attachment of embryos to the oviductal epithelia. Due to the presence of ZP, it has been difficult to transfect preimplantation embryos existing within the oviductal lumen, with exogenous nucleic acids, such as DNA and mRNA. However, intraoviductal instillation of nucleic acids, and subsequent in vivo electroporation in pregnant females, enables transfection of these embryos, leading to the production of gene-modified animals. This new method for production of genetically modified animals does not require any ex vivo handling of embryos, which has been essential for traditional transgenesis. In this article, we describe recent advances in the in vivo transfection of preimplantation mammalian embryos, and also the possibility of simple transfection of these embryos through intraoviductal instillation of a solution, alone.",book:{id:"7233",slug:"new-insights-into-theriogenology",title:"New Insights into Theriogenology",fullTitle:"New Insights into Theriogenology"},signatures:"Masahiro Sato, Masato Ohtsuka and Shingo Nakamura",authors:[{id:"177440",title:"Dr.",name:"Masato",middleName:null,surname:"Ohtsuka",slug:"masato-ohtsuka",fullName:"Masato Ohtsuka"},{id:"177444",title:"Dr.",name:"Shingo",middleName:null,surname:"Nakamura",slug:"shingo-nakamura",fullName:"Shingo Nakamura"},{id:"245795",title:"Prof.",name:"Masahiro",middleName:null,surname:"Sato",slug:"masahiro-sato",fullName:"Masahiro Sato"}]},{id:"50061",doi:"10.5772/62470",title:"Sperm Motility Regulatory Proteins: A Tool to Enhance Sperm Quality",slug:"sperm-motility-regulatory-proteins-a-tool-to-enhance-sperm-quality",totalDownloads:2126,totalCrossrefCites:2,totalDimensionsCites:6,abstract:"Sperm forward motility is an essential parameter in mammalian fertilization. Studies from our laboratory have identified and characterized a few unique sperm motility regulatory proteins/glycoproteins from the male reproductive fluids and mammalian blood serum. The purified sperm motility-initiating protein (MIP) from caprine epididymal plasma as well as the forward motility-stimulating factor (FMSF) and motility-stimulating protein (MSP) from buffalo and goat serum, respectively, have high efficacy to initiate or increase motility in nonmotile or less motile sperm. Antibody of sperm motility inhibitory factor (MIF-II) has the high potential to enhance sperm vertical velocity and forward motility by increasing intracellular cyclic adenosine monophosphate (cAMP) level. The appearance and disappearance of D-galactose–specific lectin and its receptor along the epididymis has been reported to be involved in motility regulation in spermatozoa. A novel synthetic cryopreservation method and role of lipid to protect membrane damage during cryopreservation have been demonstrated. Motility-promoting proteins may be extremely useful for improving cattle breeding and breeding of endangered species, thereby helping in enhanced production of animal products as well as in the conservation of animals. Isolated proteins and developed cryopreservation technology may also be beneficial in human infertility clinics to increase the chance of fertilization.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Sandhya R. Dungdung, Arpita Bhoumik, Sudipta Saha, Prasanta\nGhosh, Kaushik Das, Sandipan Mukherjee, Debjani Nath, Jitamanyu\nChakrabarty, Chanakyanath Kundu, Bijay Shankar Jaiswal, Mahitosh\nMandal, Arunima Maiti, Saswati Banerjee, Madhumita\nRoychowdhury, Debleena Ray, Debdas Bhattacharyya and Gopal C.\nMajumder",authors:[{id:"50052",title:"Dr.",name:"Mahitosh",middleName:null,surname:"Mandal",slug:"mahitosh-mandal",fullName:"Mahitosh Mandal"},{id:"177044",title:"Dr.",name:"Sandhya",middleName:null,surname:"Dungdung",slug:"sandhya-dungdung",fullName:"Sandhya Dungdung"},{id:"177920",title:"Dr.",name:"Arpita",middleName:null,surname:"Bhoumik",slug:"arpita-bhoumik",fullName:"Arpita Bhoumik"},{id:"177921",title:"Dr.",name:"Sudipta",middleName:null,surname:"Saha",slug:"sudipta-saha",fullName:"Sudipta Saha"},{id:"177922",title:"MSc.",name:"Prasanta",middleName:null,surname:"Ghosh",slug:"prasanta-ghosh",fullName:"Prasanta Ghosh"},{id:"177923",title:"Dr.",name:"Kaushik",middleName:null,surname:"Das",slug:"kaushik-das",fullName:"Kaushik Das"},{id:"177924",title:"MSc.",name:"Sandipan",middleName:null,surname:"Mukherjee",slug:"sandipan-mukherjee",fullName:"Sandipan Mukherjee"},{id:"177925",title:"Dr.",name:"Debjani",middleName:null,surname:"Nath",slug:"debjani-nath",fullName:"Debjani Nath"},{id:"177927",title:"Dr.",name:"Jitamanyu",middleName:null,surname:"Chakrabarty",slug:"jitamanyu-chakrabarty",fullName:"Jitamanyu Chakrabarty"},{id:"177928",title:"Dr.",name:"Chanakyanath",middleName:null,surname:"Kundu",slug:"chanakyanath-kundu",fullName:"Chanakyanath Kundu"},{id:"177929",title:"Dr.",name:"Bijay Shankar",middleName:null,surname:"Jaiswal",slug:"bijay-shankar-jaiswal",fullName:"Bijay Shankar Jaiswal"},{id:"177930",title:"Dr.",name:"Arunima",middleName:null,surname:"Maiti",slug:"arunima-maiti",fullName:"Arunima Maiti"},{id:"177931",title:"Dr.",name:"Saswati",middleName:null,surname:"Banerjee",slug:"saswati-banerjee",fullName:"Saswati Banerjee"},{id:"177932",title:"Dr.",name:"Madhumita",middleName:null,surname:"Roychowdhury",slug:"madhumita-roychowdhury",fullName:"Madhumita Roychowdhury"},{id:"177933",title:"MSc.",name:"Debleena",middleName:null,surname:"Ray",slug:"debleena-ray",fullName:"Debleena Ray"},{id:"177934",title:"Dr.",name:"Debdas",middleName:null,surname:"Bhattacharyya",slug:"debdas-bhattacharyya",fullName:"Debdas Bhattacharyya"},{id:"177935",title:"Dr.",name:"Gopal Chandra",middleName:null,surname:"Majumder",slug:"gopal-chandra-majumder",fullName:"Gopal Chandra Majumder"}]},{id:"63404",doi:"10.5772/intechopen.80229",title:"Subclinical Endometritis in Dairy Cattle",slug:"subclinical-endometritis-in-dairy-cattle",totalDownloads:1800,totalCrossrefCites:1,totalDimensionsCites:5,abstract:"Subclinical endometritis is recognized as a cause of poor reproductive performance in dairy cows. Inflammation of the endometrium persisting after postpartum uterine involution has been related with prolonged calving-conception intervals and low fertility in dairy cows. The subclinical nature of this condition makes it necessary in the use of endometrial cytology or biopsy for diagnosing it. There are some controversies among authors in relation to the postpartum period from which a physiological endometrial inflammation should be considered a pathological subclinical endometritis. Therefore, depending on the sampling period after calving, different studies establish a different degree of polymorphonuclear leukocyte infiltration as cutoff point to diagnose subclinical endometritis. Controversies also exist regarding the pathogenesis of the disease and its consequences on the fertility of dairy cattle. The aim of this chapter was to review the current knowledge on this uterine pathology.",book:{id:"7233",slug:"new-insights-into-theriogenology",title:"New Insights into Theriogenology",fullTitle:"New Insights into Theriogenology"},signatures:"Luis Angel Quintela Arias, Marcos Vigo Fernández, Juan José\nBecerra González, Mónica Barrio López, Pedro José García Herradón\nand Ana Isabel Peña Martínez",authors:[{id:"243272",title:"Prof.",name:"Luis Angel",middleName:null,surname:"Quintela Arias",slug:"luis-angel-quintela-arias",fullName:"Luis Angel Quintela Arias"},{id:"243886",title:"Prof.",name:"Ana Isabel",middleName:null,surname:"Peña Martínez",slug:"ana-isabel-pena-martinez",fullName:"Ana Isabel Peña Martínez"},{id:"243887",title:"Prof.",name:"Pedro",middleName:null,surname:"García Herradón",slug:"pedro-garcia-herradon",fullName:"Pedro García Herradón"},{id:"243888",title:"Prof.",name:"Juan José",middleName:null,surname:"Becerra González",slug:"juan-jose-becerra-gonzalez",fullName:"Juan José Becerra González"},{id:"256852",title:"Dr.",name:"Mónica",middleName:null,surname:"Barrio López",slug:"monica-barrio-lopez",fullName:"Mónica Barrio López"},{id:"256854",title:"Dr.",name:"Marcos",middleName:null,surname:"Vigo Fernández",slug:"marcos-vigo-fernandez",fullName:"Marcos Vigo Fernández"}]}],mostDownloadedChaptersLast30Days:[{id:"49736",title:"Chromosome Abnormalities in Domestic Animals as Causes of Disorders of Sex Development or Impaired Fertility",slug:"chromosome-abnormalities-in-domestic-animals-as-causes-of-disorders-of-sex-development-or-impaired-f",totalDownloads:4132,totalCrossrefCites:7,totalDimensionsCites:20,abstract:"Cytogenetic evaluation is an important step in the diagnosis of infertile or sterile animals. Moreover, the analysis of sex chromosomes is crucial for a proper classification of disorders of sex development (DSD). For many years, chromosome studies mainly addressed the livestock species, while recently, increasing interest in such analysis in companion animals is observed. New molecular and cytogenetic tools and techniques have given opportunities for a precise identification of chromosome mutations. Among them, fluorescence in situ hybridization, besides chromosome banding, has become a gold standard. In this chapter, recent advances in the cytogenetic diagnosis of cattle, pigs, horses, dogs and cats are presented.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Izabela Szczerbal and Marek Switonski",authors:[{id:"177030",title:"Prof.",name:"Marek",middleName:null,surname:"Switonski",slug:"marek-switonski",fullName:"Marek Switonski"},{id:"177045",title:"Dr.",name:"Izabela",middleName:null,surname:"Szczerbal",slug:"izabela-szczerbal",fullName:"Izabela Szczerbal"}]},{id:"50144",title:"Proliferative Endometrial Lesions Hidden behind the Feline Pyometra",slug:"proliferative-endometrial-lesions-hidden-behind-the-feline-pyometra",totalDownloads:2456,totalCrossrefCites:0,totalDimensionsCites:4,abstract:"The literature refers to pyometra as the most important pathology in the feline uterus, which is often associated with cystic endometrial disease (cystic endometrial hyperplasia/pyometra complex or CEH-Pyo). The etiology of pyometra is complex and probably multifactorial, but hormonal influences are suggested to play an important role in the pathogenesis. Progestagen-based contraceptives may be risk factors for the CEH-Pyo syndrome, for endometrial adenocarcinoma and also to mammary tumors in this species.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Maria dos Anjos Pires, Hugo Vilhena, Sónia Miranda, Miguel\nTavares Pereira, Fernanda Seixas and Ana Laura Saraiva",authors:[{id:"41065",title:"Dr.",name:"Sónia",middleName:null,surname:"Miranda",slug:"sonia-miranda",fullName:"Sónia Miranda"},{id:"161556",title:"Dr.",name:"Maria Dos Anjos",middleName:null,surname:"Pires",slug:"maria-dos-anjos-pires",fullName:"Maria Dos Anjos Pires"},{id:"179547",title:"MSc.",name:"Hugo",middleName:null,surname:"Vilhena",slug:"hugo-vilhena",fullName:"Hugo Vilhena"},{id:"179548",title:"MSc.",name:"Miguel",middleName:null,surname:"Tavares Pereira",slug:"miguel-tavares-pereira",fullName:"Miguel Tavares Pereira"},{id:"179549",title:"Prof.",name:"Fernanda",middleName:null,surname:"Seixas",slug:"fernanda-seixas",fullName:"Fernanda Seixas"},{id:"179550",title:"Prof.",name:"Ana Laura",middleName:null,surname:"Saraiva",slug:"ana-laura-saraiva",fullName:"Ana Laura Saraiva"}]},{id:"49944",title:"The Use of Reproductive Technologies to Produce Transgenic Goats",slug:"the-use-of-reproductive-technologies-to-produce-transgenic-goats",totalDownloads:2411,totalCrossrefCites:2,totalDimensionsCites:3,abstract:"Recombinant DNA technology has revolutionized the production of therapeutic proteins. Thus, genes of a great number of human proteins have already been identified and cloned. The use of farm animals as bioreactors may be the better choice to produce recombinant therapeutic proteins. For this activity, the term “pharming” was created, referring to the use of genetic engineering to obtain a transgenic or genetically modified animal. Considering the advantages and disadvantages of livestock species, goats appear as a very good model. In addition, the first human commercially approved biological drug (antithrombin (AT)) was produced from the milk of transgenic goats. The aim of this chapter is to present various reproductive technologies used to obtain transgenic goats secreting recombinant proteins in milk. Initially, this chapter presents the methods for embryo production (in vivo and in vitro) to realize the DNA microinjection in pronuclear embryos. Thus, the techniques of superovulation of donors (in vivo embryo production) and ovarian stimulation for oocyte recovery (in vitro embryo production) are described. Also, the methods for DNA microinjection and embryo transfer are detailed in this chapter. Finally, this chapter describes the reproductive procedures used for obtaining transgenic goats by cloning.",book:{id:"5105",slug:"insights-from-animal-reproduction",title:"Insights from Animal Reproduction",fullTitle:"Insights from Animal Reproduction"},signatures:"Vicente J. F. Freitas, Luciana M. Melo, Dárcio I.A. Teixeira, Maajid H.\nBhat, Irina A. Serova, Lyudmila E. Andreeva and Oleg L. Serov",authors:[{id:"177122",title:"Dr.",name:"Vicente",middleName:null,surname:"Freitas",slug:"vicente-freitas",fullName:"Vicente Freitas"},{id:"177194",title:"Dr.",name:"Luciana",middleName:null,surname:"Melo",slug:"luciana-melo",fullName:"Luciana Melo"},{id:"177195",title:"Dr.",name:"Dárcio",middleName:null,surname:"Teixeira",slug:"darcio-teixeira",fullName:"Dárcio Teixeira"},{id:"177196",title:"Dr.",name:"Maajid",middleName:null,surname:"Bhat",slug:"maajid-bhat",fullName:"Maajid Bhat"},{id:"185365",title:"Dr.",name:"Irina",middleName:null,surname:"Aleksandrovna SEROVA",slug:"irina-aleksandrovna-serova",fullName:"Irina Aleksandrovna SEROVA"},{id:"185366",title:"Dr.",name:"Lyudmila",middleName:null,surname:"Evgenievna ANDREEVA",slug:"lyudmila-evgenievna-andreeva",fullName:"Lyudmila Evgenievna ANDREEVA"},{id:"185367",title:"Dr.",name:"Oleg",middleName:null,surname:"Leonidovich SEROV",slug:"oleg-leonidovich-serov",fullName:"Oleg Leonidovich SEROV"}]},{id:"63404",title:"Subclinical Endometritis in Dairy Cattle",slug:"subclinical-endometritis-in-dairy-cattle",totalDownloads:1800,totalCrossrefCites:1,totalDimensionsCites:5,abstract:"Subclinical endometritis is recognized as a cause of poor reproductive performance in dairy cows. Inflammation of the endometrium persisting after postpartum uterine involution has been related with prolonged calving-conception intervals and low fertility in dairy cows. The subclinical nature of this condition makes it necessary in the use of endometrial cytology or biopsy for diagnosing it. There are some controversies among authors in relation to the postpartum period from which a physiological endometrial inflammation should be considered a pathological subclinical endometritis. Therefore, depending on the sampling period after calving, different studies establish a different degree of polymorphonuclear leukocyte infiltration as cutoff point to diagnose subclinical endometritis. Controversies also exist regarding the pathogenesis of the disease and its consequences on the fertility of dairy cattle. The aim of this chapter was to review the current knowledge on this uterine pathology.",book:{id:"7233",slug:"new-insights-into-theriogenology",title:"New Insights into Theriogenology",fullTitle:"New Insights into Theriogenology"},signatures:"Luis Angel Quintela Arias, Marcos Vigo Fernández, Juan José\nBecerra González, Mónica Barrio López, Pedro José García Herradón\nand Ana Isabel Peña Martínez",authors:[{id:"243272",title:"Prof.",name:"Luis Angel",middleName:null,surname:"Quintela Arias",slug:"luis-angel-quintela-arias",fullName:"Luis Angel Quintela Arias"},{id:"243886",title:"Prof.",name:"Ana Isabel",middleName:null,surname:"Peña Martínez",slug:"ana-isabel-pena-martinez",fullName:"Ana Isabel Peña Martínez"},{id:"243887",title:"Prof.",name:"Pedro",middleName:null,surname:"García Herradón",slug:"pedro-garcia-herradon",fullName:"Pedro García Herradón"},{id:"243888",title:"Prof.",name:"Juan José",middleName:null,surname:"Becerra González",slug:"juan-jose-becerra-gonzalez",fullName:"Juan José Becerra González"},{id:"256852",title:"Dr.",name:"Mónica",middleName:null,surname:"Barrio López",slug:"monica-barrio-lopez",fullName:"Mónica Barrio López"},{id:"256854",title:"Dr.",name:"Marcos",middleName:null,surname:"Vigo Fernández",slug:"marcos-vigo-fernandez",fullName:"Marcos Vigo Fernández"}]},{id:"79909",title:"Cryopreservation Methods and Frontiers in the Art of Freezing Life in Animal Models",slug:"cryopreservation-methods-and-frontiers-in-the-art-of-freezing-life-in-animal-models",totalDownloads:159,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"The development in cryobiology in animal breeding had revolutionized the field of reproductive medicine. The main objective to preserve animal germplasm stems from variety of reasons such as conservation of endangered animal species, animal diversity, and an increased demand of animal models and/or genetically modified animals for research involving animal and human diseases. Cryopreservation has emerged as promising technique for fertility preservation and assisted reproduction techniques (ART) for production of animal breeds and genetically engineered animal species for research. Slow rate freezing and rapid freezing/vitrification are the two main methods of cryopreservation. Slow freezing is characterized by the phase transition (liquid turning into solid) when reducing the temperature below freezing point. Vitrification, on the other hand, is a phenomenon in which liquid solidifies without the formation of ice crystals, thus the process is referred to as a glass transition or ice-free cryopreservation. The vitrification protocol applies high concentrations of cryoprotective agents (CPA) used to avoid cryoinjury. This chapter provides a brief overview of fundamentals of cryopreservation and established methods adopted in cryopreservation. Strategies involved in cryopreserving germ cells (sperm and egg freezing) are included in this chapter. Last section describes the frontiers and advancement of cryopreservation in some of the important animal models like rodents (mouse and rats) and in few large animals (sheep, cow etc).",book:{id:"10664",slug:null,title:"Animal Reproduction",fullTitle:"Animal Reproduction"},signatures:"Feda S. Aljaser",authors:null}],onlineFirstChaptersFilter:{topicId:"300",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"79909",title:"Cryopreservation Methods and Frontiers in the Art of Freezing Life in Animal Models",slug:"cryopreservation-methods-and-frontiers-in-the-art-of-freezing-life-in-animal-models",totalDownloads:161,totalDimensionsCites:0,doi:"10.5772/intechopen.101750",abstract:"The development in cryobiology in animal breeding had revolutionized the field of reproductive medicine. The main objective to preserve animal germplasm stems from variety of reasons such as conservation of endangered animal species, animal diversity, and an increased demand of animal models and/or genetically modified animals for research involving animal and human diseases. Cryopreservation has emerged as promising technique for fertility preservation and assisted reproduction techniques (ART) for production of animal breeds and genetically engineered animal species for research. Slow rate freezing and rapid freezing/vitrification are the two main methods of cryopreservation. Slow freezing is characterized by the phase transition (liquid turning into solid) when reducing the temperature below freezing point. Vitrification, on the other hand, is a phenomenon in which liquid solidifies without the formation of ice crystals, thus the process is referred to as a glass transition or ice-free cryopreservation. The vitrification protocol applies high concentrations of cryoprotective agents (CPA) used to avoid cryoinjury. This chapter provides a brief overview of fundamentals of cryopreservation and established methods adopted in cryopreservation. Strategies involved in cryopreserving germ cells (sperm and egg freezing) are included in this chapter. Last section describes the frontiers and advancement of cryopreservation in some of the important animal models like rodents (mouse and rats) and in few large animals (sheep, cow etc).",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Feda S. Aljaser"},{id:"79782",title:"Avian Reproduction",slug:"avian-reproduction",totalDownloads:149,totalDimensionsCites:0,doi:"10.5772/intechopen.101185",abstract:"There are about 10,400 living avian species belonging to the class Aves, characterized by feathers which no other animal classes possess and are warm-blooded vertebrates with four-chamber heart. They have excellent vision, and their forelimbs are modified into wings for flight or swimming, though not all can fly or swim. They lay hard-shelled eggs which are a secretory product of the reproductive system that vary greatly in colour, shape and size, and the bigger the bird, the bigger the egg. Since domestication, avian species have been basically reared for eggs, meat, pleasure and research. They reproduce sexually with the spermatozoa being homogametic and carry Z-bearing chromosomes, and the blastodisk carries either Z-bearing or W-bearing chromosomes, hence, the female is heterogametic, and thus, determines the sex of the offspring. The paired testes produce spermatozoa, sex hormones and the single ovary (with a few exceptions) produces yolk bearing the blastodisk and sex hormones. Both testis and ovary are the primary sex organs involved in sexual characteristics development in avian. In avian reproduction, there must be mating for fertile egg that must be incubated to produce the young ones. At hatch, hatchling sex is identified and reared to meet the aim of the farmer.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Kingsley Omogiade Idahor"},{id:"78802",title:"Intraovarian Gestation in Viviparous Teleosts: Unique Type of Gestation among Vertebrates",slug:"intraovarian-gestation-in-viviparous-teleosts-unique-type-of-gestation-among-vertebrates",totalDownloads:184,totalDimensionsCites:0,doi:"10.5772/intechopen.100267",abstract:"The intraovarian gestation, occurring in teleosts, makes this type of reproduction a such complex and unique condition among vertebrates. This type of gestation of teleosts is expressed in special morphological and physiological characteristic where occurs the viviparity and it is an essential component in the analysis of the evolutionary process of viviparity in vertebrates. In viviparous teleosts, during embryogenesis, there are not development of Müllerian ducts, which form the oviducts in the rest of vertebrates, as a result, exclusively in teleosts, there are not oviducts and the caudal region of the ovary, the gonoduct, connects the ovary to the exterior. The lack of oviducts defines that the embryos develop into the ovary, as intraovarian gestation. The ovary forms the oocytes which may develop different type of oogenesis, according with the storage of diverse amount of yolk, variation observed corresponding to the species. The viviparous gestation is characterized by the possible intimate contact between maternal and embryonic tissues, process that permits their metabolic interchanges. So, the nutrients obtained by the embryos could be deposited in the oocyte before fertilization, contained in the yolk (lecithotrophy), and may be completed during gestation by additional provisioning from maternal tissues to the embryo (matrotrophy). Then, essential requirements for viviparity in poeciliids and goodeids are characterized by: a) the diversification of oogenesis, with the deposition of different amount of yolk in the oocyte; b) the insemination, by the transfer of sperm to the female gonoduct and their transportation from the gonoduct to the germinal region of the ovary where the follicles develop; c) the intrafollicular fertilization; d) the intraovarian gestation with the development of embryos in intrafollicular gestation (as in poeciliids), or intraluminal gestation (as in goodeids); and, e) the origin of embryonic nutrition may be by lecithotrophy and matrotrophy. The focus of this revision compares the general and specific structural characteristics of the viviparity occurring into the intraovarian gestation in teleosts, defining this reproductive strategy, illustrated in this review with histological material in a poeciliid, of the species Poecilia latipinna (Lesueur, 1821) (Poeciliidae), and in a goodeid, of the species Xenotoca eiseni (Rutter, 1896) (Goodeidae).",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Mari-Carmen Uribe, Gabino De la Rosa-Cruz, Adriana García-Alarcón and Juan Carlos Campuzano-Caballero"},{id:"78617",title:"Doppler Ultrasound in the Reproduction of Mares",slug:"doppler-ultrasound-in-the-reproduction-of-mares",totalDownloads:122,totalDimensionsCites:0,doi:"10.5772/intechopen.98951",abstract:"Doppler ultrasonographic (US) is a method that provides real-time information on vascular architecture and hemodynamic aspects of blood vessels. It can determine the presence, direction, and speed of blood flow, being subdivided into the categories of color Doppler (color flow and power flow) and pulsed Doppler. The objective of this chapter was to compile data from several studies addressing the use of US Doppler correlated with pathophysiological phenomena of equine reproduction. Initially we decided to describe the technique, advantages, and disadvantages of each Doppler mode. Then the applicability of US Doppler in mares related to equine reproduction. Thus, within this chapter, you will find the form of use and descriptions of studies carried out on vascular perfusion of the follicular dynamics, the corpus luteum, the uterine segments, which we have divided into post-insemination evaluation, endometritis diagnosis and pregnancy diagnosis. So, we hope that this chapter will expand the knowledge about US Doppler and increase the number of veterinarians who will introduce the technique into their practical routine.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Camila Silva Costa Ferreira and Rita de Cássia Lima Morais"},{id:"78202",title:"Stimulatory Effects of Androgens on Eel Primary Ovarian Development - from Phenotypes to Genotypes",slug:"stimulatory-effects-of-androgens-on-eel-primary-ovarian-development-from-phenotypes-to-genotypes",totalDownloads:138,totalDimensionsCites:0,doi:"10.5772/intechopen.99582",abstract:"Androgens stimulate primary ovarian development in Vertebrate. Japanese eels underwent operation to sample the pre- and post-treated ovarian tissues from the same individual. Ovarian phenotypic or genotypic data were mined in a pair. A correlation between the initial ovarian status (determined by kernel density estimation (KDE), presented as a probability density of oocyte size) and the consequence of androgen (17MT) treatment (change in ovary) has been showed. The initial ovarian status appeared to be important to influence ovarian androgenic sensitivity. The initial ovary was important to the outcomes of androgen treatments, and ePAV (expression presence-absence variation) is existing in Japanese eel by analyze DEGs; core, unique, or accessory genes were identified, the sensitivities of initial ovaries were correlated with their gene expression profiles. We speculated the importance of genetic differential expression on the variations of phenotypes by 17MT, and transcriptomic approach seems to allow extracting multiple layers of genomic data.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Yung-Sen Huang and Chung-Yen Lin"},{id:"78116",title:"Embryo Transfer",slug:"embryo-transfer",totalDownloads:255,totalDimensionsCites:1,doi:"10.5772/intechopen.99683",abstract:"Assisted reproductive technologies (ART) have made tremendous advances, in last years. Artificial insemination is a method for achieving slow genetic progress in populations of animals. Many large and small ruminants are bred by AI, and more than a half million embryos are transferred every year around the world. Most of the ruminants sires used for artificial insemination were derived from embryo transfer. Improvements of reproductive biotechnologies of controlling the estrous cycle and ovulation have resulted in more effective programs for AI, superovulation of donor, and the management of ET. In the ruminants, ET procedure is a timely alternative that can allow good conception rates to be obtained constant in a year. There have been great advances of this biotechnique with on aimed to intensify the genetic progress between generations of farm. The gains is possible with the development of advanced reproductive biotechnique. The best current strategy in applying biotechnology to farmers is to use AI with sexed semen, so farmers will enjoy and benefit. The use of ET together with cryopreserved sexed embryos has a very specific potential for donor replacement and genetic improvement of the herd. In this chapter, procedures of the MOET protocol were described step by step.",book:{id:"10664",title:"Animal Reproduction",coverURL:"https://cdn.intechopen.com/books/images_new/10664.jpg"},signatures:"Ștefan Gregore Ciornei"}],onlineFirstChaptersTotal:7},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:8,numberOfPublishedChapters:87,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:98,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:27,numberOfPublishedChapters:286,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:11,numberOfPublishedChapters:139,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:8,numberOfPublishedChapters:129,numberOfOpenTopics:0,numberOfUpcomingTopics:2,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!1},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:106,numberOfOpenTopics:3,numberOfUpcomingTopics:1,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:9,numberOfPublishedChapters:101,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:11,numberOfOpenTopics:2,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:0,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!1},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:0,numberOfPublishedChapters:9,numberOfOpenTopics:4,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. The whole process of submitting an article and editing of the submitted article goes extremely smooth and fast, the number of reads and downloads of chapters is high, and the contributions are also frequently cited.",author:{id:"55578",name:"Antonio",surname:"Jurado-Navas",institutionString:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRisIQAS/Profile_Picture_1626166543950",slug:"antonio-jurado-navas",institution:{id:"720",name:"University of Malaga",country:{id:null,name:"Spain"}}}},{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}}]},series:{item:{id:"24",title:"Sustainable Development",doi:"10.5772/intechopen.100361",issn:null,scope:"