Quantity of confined cattle, semi-confined cattle and total cattle herd with some type of confinement in Brazil, 2000–2015.
\r\n\tAccording to the protection and control strategies in recent years; Although WHO has reduced the rates somewhat with the application of mass medication in children in places where the prevalence of roundworm is over 20%, to control morbidity and eliminate STN as a public health problem, the mathematical applications have been to apply the treatments to adults as well.
\r\n\r\n\tIn this book, roundworms transmitted through soil or arthropods; Developments in epidemiology, life cycles, pathophysiology, clinical diagnosis, management, and public health control in the world will be reviewed with the contribution of studies on this subject from past to present. In addition, this book aims to highlight the connection between helminths and autoimmune and allergic diseases: the determination, treatment, and control strategies.
",isbn:"978-1-80356-714-3",printIsbn:"978-1-80356-713-6",pdfIsbn:"978-1-80356-715-0",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,hash:"5edc96349630be8bb4e67170be677d8c",bookSignature:"Dr. Nihal Dogan",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11801.jpg",keywords:"Ascaris, Trichuris, Hookworms, Strongyloides, Wuchereria, Brugia, Onchocerca, Trichinella, Larval Infection, Visceral Larva Migrans, Cutaneous Larva Migrans, Ocular",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 23rd 2022",dateEndSecondStepPublish:"May 27th 2022",dateEndThirdStepPublish:"July 26th 2022",dateEndFourthStepPublish:"October 14th 2022",dateEndFifthStepPublish:"December 13th 2022",remainingDaysToSecondStep:"7 days",secondStepPassed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"A leading academic in parasitology at the Department of Microbiology at the Faculty of Medicine of Eskişehir Osmangazi University, expertise in hydatid cysts, toxoplasma, leishmania, parasitic diseases transmitted by water and intestinal parasites. She wrote numerous book chapters on infectious diseases, clinical parasitology, clinical microbiology, and medical microbiology laboratory applications and manuals.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"169552",title:"Dr.",name:"Nihal",middleName:null,surname:"Dogan",slug:"nihal-dogan",fullName:"Nihal Dogan",profilePictureURL:"https://mts.intechopen.com/storage/users/169552/images/system/169552.png",biography:"Prof. Dr Nihal Doğan is the leading academic in the Field of Parasitology at the Department of Microbiology at the Faculty of Medicine of Eskişehir Osmangazi University since 1993. She was granted a professorship in 2008 and has expertise in parasitology and epidemiology of parasitic diseases. She took part as an executive academic on 6 projects hydatid cysts, toxoplasma, leishmania, parasitic diseases transmitted by water and intestinal parasites. Her research is published in more than 40 national and international journals and she took part as a keynote speaker and as abstract and poster presenter in more than international and national congresses and conferences. She wrote numerous book chapters on infectious diseases, clinical parasitology, clinical microbiology and medical microbiology laboratory applications and manuals. \nShe concluded her Master and PhD Thesis at Eskişehir Anadolu University and Eskişehir Osmangazi University Medical Faculty and focused on the field of diagnosis and seroepidemiology of Toxoplasmosis. She visited the University of Virginia Department of Parasitology as a visiting researcher in 2003 for 3 months and worked on the diagnosis of Entamoeba histolytica and Universidad De Chile Faculty of Medicine as an observer researcher in 2016 for 1 month and worked on Trypanosomes.\nHer research interests include medical ethics, seroepidemiological survey; intestinal, blood, tissue and ocular parasites, vector-borne diseases, zoonotic parasites.",institutionString:"Eskisehir Osmangazi University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"0",totalChapterViews:"0",totalEditedBooks:"0",institution:null}],coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"13",title:"Immunology and Microbiology",slug:"immunology-and-microbiology"}],chapters:null,productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"466998",firstName:"Dragan",lastName:"Miljak",middleName:"Anton",title:"Mr.",imageUrl:"https://mts.intechopen.com/storage/users/466998/images/21564_n.jpg",email:"dragan@intechopen.com",biography:"As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. 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Venkateswarlu",coverURL:"https://cdn.intechopen.com/books/images_new/371.jpg",editedByType:"Edited by",editors:[{id:"58592",title:"Dr.",name:"Arun",surname:"Shanker",slug:"arun-shanker",fullName:"Arun Shanker"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"72",title:"Ionic Liquids",subtitle:"Theory, Properties, New Approaches",isOpenForSubmission:!1,hash:"d94ffa3cfa10505e3b1d676d46fcd3f5",slug:"ionic-liquids-theory-properties-new-approaches",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/72.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"314",title:"Regenerative Medicine and Tissue Engineering",subtitle:"Cells and Biomaterials",isOpenForSubmission:!1,hash:"bb67e80e480c86bb8315458012d65686",slug:"regenerative-medicine-and-tissue-engineering-cells-and-biomaterials",bookSignature:"Daniel Eberli",coverURL:"https://cdn.intechopen.com/books/images_new/314.jpg",editedByType:"Edited by",editors:[{id:"6495",title:"Dr.",name:"Daniel",surname:"Eberli",slug:"daniel-eberli",fullName:"Daniel Eberli"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"57",title:"Physics and Applications of Graphene",subtitle:"Experiments",isOpenForSubmission:!1,hash:"0e6622a71cf4f02f45bfdd5691e1189a",slug:"physics-and-applications-of-graphene-experiments",bookSignature:"Sergey Mikhailov",coverURL:"https://cdn.intechopen.com/books/images_new/57.jpg",editedByType:"Edited by",editors:[{id:"16042",title:"Dr.",name:"Sergey",surname:"Mikhailov",slug:"sergey-mikhailov",fullName:"Sergey Mikhailov"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"1373",title:"Ionic Liquids",subtitle:"Applications and Perspectives",isOpenForSubmission:!1,hash:"5e9ae5ae9167cde4b344e499a792c41c",slug:"ionic-liquids-applications-and-perspectives",bookSignature:"Alexander Kokorin",coverURL:"https://cdn.intechopen.com/books/images_new/1373.jpg",editedByType:"Edited by",editors:[{id:"19816",title:"Prof.",name:"Alexander",surname:"Kokorin",slug:"alexander-kokorin",fullName:"Alexander Kokorin"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"2270",title:"Fourier Transform",subtitle:"Materials Analysis",isOpenForSubmission:!1,hash:"5e094b066da527193e878e160b4772af",slug:"fourier-transform-materials-analysis",bookSignature:"Salih Mohammed Salih",coverURL:"https://cdn.intechopen.com/books/images_new/2270.jpg",editedByType:"Edited by",editors:[{id:"111691",title:"Dr.Ing.",name:"Salih",surname:"Salih",slug:"salih-salih",fullName:"Salih Salih"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}},{type:"book",id:"872",title:"Organic Pollutants Ten Years After the Stockholm Convention",subtitle:"Environmental and Analytical Update",isOpenForSubmission:!1,hash:"f01dc7077e1d23f3d8f5454985cafa0a",slug:"organic-pollutants-ten-years-after-the-stockholm-convention-environmental-and-analytical-update",bookSignature:"Tomasz Puzyn and Aleksandra Mostrag-Szlichtyng",coverURL:"https://cdn.intechopen.com/books/images_new/872.jpg",editedByType:"Edited by",editors:[{id:"84887",title:"Dr.",name:"Tomasz",surname:"Puzyn",slug:"tomasz-puzyn",fullName:"Tomasz Puzyn"}],productType:{id:"1",chapterContentType:"chapter",authoredCaption:"Edited by"}}]},chapter:{item:{type:"chapter",id:"79800",title:"Biogas Generation from Bovine Confinement: An Energy Policy Option for Brazil",doi:"10.5772/intechopen.99828",slug:"biogas-generation-from-bovine-confinement-an-energy-policy-option-for-brazil",body:'With one of the largest cattle herds in the world, with almost 215.2 million heads1 according to FAO [1] and IBGE data [2], with almost 90% of this being beef cattle (approximately 193.5 million heads of cattle) and almost 22 million head of dairy cattle, in 2015, the production of beef and dairy products, largely based on extensive systems, has a significant environmental impact, including with a large demand for energy resources, most of which is non-renewable and of fossil origin. The Figure 1 shows countries with the largest cattle herds, 2000-2014.
Countries with the largest cattle herds, 2000–2014, in millions of heads. Source: Prepared by the authors, based on FAO [
Agriculture is one of the principal GHG emitting activities in Brazil due to, for example, the methane resulting principally from enteric fermentation and the handling of waste, and nitrous oxide from feces and urine deposited by animals [3]. In the last emissions inventory, agriculture was responsible for around 32% of greenhouse gas emissions in the country, without taking into account the emissions created from the use of energy, measured in the part of energy whose contribution is almost 29%, but which includes a part related to the demand of the agricultural sector for energy and energy resources, as well as emissions from the conversion of forests into pasture, calculated in the part related to changes in land use. Agricultural thus contributes, directly or indirectly, to most greenhouse gases produced in the country, as seen in Figure 2.
Participation of each sector in the GEE emissions, in CO2e, in 2010. Source: [
In relation to the demand for energy, the agricultural sector demanded around 14.08% of the total diesel oil used in the country (approximately 7460,000 m3) and almost 11.48% of firewood (more than 9000 tons) [4]. In the case of the use of electric energy, the agricultural sector consumed around 5.14% of the final total consumption of electricity in 2015, or 26,870.89 GWh [4].
Despite have a majority renewable energy matrix, as D’Avignon [5] highlights, this does not impede the need for the implementation of complementary renewable energy programs, above all with short-term energy policies, given the environmental limitations on the construction of new hydro-electric power plants with large reservoirs2, which include the majority of those constructed in Brazil, or the hydric crises which have occurred in recent years, causing serious problems with the supply of electric energy, imposing the need, for example, for rationing and the introduction of non-renewable sources of energy and more sources of GHG emissions, following the insertion of thermoelectric power plants (above all, coal and oil) [6, 7, 8, 9, 10]. A way to reduce dependence on hydroelectricity for the generation of electric energy and with a lower environmental impact is a policy of complementarity with new sources of renewable energy generation [5]. One of the possible renewable energy sources is biogas produced through the confinement of cattle.
According to the FAO [11], it is estimated that livestock emit around 7.1 GgCO2eq per year or 14.5% of total global emissions. The same study demonstrates that most emissions are related to the production of beef (41%) and milk (20%). Emissions are also linked to the production and processing of animal feed, enteric fermentation, the handling of waste and the remnants of processing, and the transport of products of an animal origin [11]. In the Brazilian case, in the last inventory, emissions related to enteric fermentation accounted for 11,158 GgCH4 or around 66.9% of total methane emissions in the country in 2010 and in the case of animal waste, this value was 3.6% of total methane emissions, 608.1 GgCH4 [12].
Confinement can contribute to the generation of electric energy with lower GHG emissions. This reduction is due to the conversion of methane generated during the beef production process into biogas as a source of energy for the energy sector, slaughterhouses, and abattoirs in particular, part of the highly energy intensive agricultural sector. At the same time, as it is a system with higher production costs in relation to the extensive system3, biogas also contributes to reducing these costs. The Figure 3 shows how the confinement rate in Brazil is still very low.
Participation of cattle in confinement system and cattle in extensive system or rational grazing system on the total Brazilian cattle herd, 2000-2015. Source: Prepared by the authors, based on FNP (2002, 2005, 2012 and 2016).
Biogas is a gaseous mix produced by the anaerobic decomposition of organic material whose characteristics and typical composition, according to Persson et al. [14], is around 53–70% methane and 30–47% carbon dioxide, with traces of at least 1000 ppm of sulfuric acid and at least 100 ppm of ammonia. Agricultural waste treatment systems are principally responsible for the use of the anaerobic digestion process [15].
Biogas is widely used in some countries, such as China and India, for cooking and lighting in rural areas. In Brazil, the use of this energy source was intensified in the 1970s, through governmental programs in small rural properties to reduce the dependence on LPG and the negative impacts of raising animals, as well as to increase the income of landowners. However, the lack of technical knowledge to construct and operate bio-digesters was one of the principal problems of the use of biogas in the country [16, 17, 18]. Usually, the most common uses of biogas, seen in a wide variety of countries, is its use for heating and the generation of electricity. In the case of the use of biogas for electricity generation, various technologies are available, with the principal applications being gas turbines and internal combustion generators [19].
The generation of biogas from animal waste depends on different factors: temperature, pH, alkalinity, the characteristics of animal waste, and how it is handled in the production system. As a result, animal diet gives the waste distinct potentials to produce gas [16, 17, 18]. The production of biogas is possible with the confinement of cattle before slaughter and from effluents from the production process in slaughterhouses and abattoirs.
Two types of confinement are practiced in Brazil [12]. Confinement and semi-confinement during the dry periods of the year, from May/June to October/November. For the confined cattle, the fattening period lasts on average four months, and two for semi-confined. Table 1 shows the growth of the confinement system in the country in recent years. In 2000, the number was 4.39 million, of which 55.6% were semi-confined and 44.4% confined. In 2015, it reached a little more than 6.7 million, with approximately 40.3% being semi-confined cattle and almost 59.7% confined. In the 2000–2015 period, there was an increase of 34.6% [20, 21, 22, 23].
Type of production system | 2000 | 2001 | 2002 | 2003 | 2004 | 2005 | 2006 | 2007 |
---|---|---|---|---|---|---|---|---|
Confinement | 1,950,000 | 1,868,000 | 1,906,000 | 2,039,000 | 2,427,000 | 2,305,000 | 2,317,999 | 2,572,984 |
Semi-confinement | 2,440,000 | 2,565,000 | 2,432,000 | 2,310,000 | 2,726,000 | 2,481,000 | 2,365,160 | 2,504,000 |
Total (confinement +semi-confinement) | 4,390,000 | 4,433,000 | 4,338,000 | 4,349,000 | 5,153,000 | 4,786,000 | 4,683,159 | 5,076,984 |
Confinement | 2,989,008 | 2,901,734 | 2,756,201 | 3,377,311 | 3,670,401 | 4,068,628 | 4,201,734 | 4,008,764 |
Semi-confinement | 2,804,000 | 2,533,191 | 2,583,042 | 2,564,146 | 2,653,589 | 2,730,584 | 2,800,802 | 2,702,774 |
Total (confinement +semi-confinement) | 5,793,008 | 5,434,925 | 5,339,243 | 5,941,457 | 6,323,990 | 6,799,212 | 7,002,536 | 6,711,538 |
The largest producers of beef in the world, the United States and Australia [24], have an average rate of confined cattle of around 50%, some years some US states, such as Texas, have an even higher rate. In the Brazilian case, this rate was 3.5% in 2016. Few states had rates much higher than the national average, notably São Paulo with 4.8% of the national herd and a confinement rate of 10.4% of its total herd. However, in many states there is little or no confinement though they have, at the same time, a large share of the national herd, as is the case of the state of Pará with approximately 10.1% of the total of the national beef herd in 2015 and with less than 1% of the cattle in the state being confined. This panorama shows the possibility of expanding beef cattle confinement in Brazil in comparison with the rates of confinement in some Brazilian states, but above all in various other countries.
The greater economic competitiveness of the extensive pasture system in relation to the intensive one with confinement can be compensated by the increase of beef production and the reduction of costs through the production of a new energy source (biogas), as well as the reduction in the emissions produced using this source of energy, and a lower dependence on electricity by the agricultural sector. Biogas can thus be an interesting and strategically positive energy policy.
To calculate the potential to generate biogas from waste created during the beef production chain and the reduction of GHG in different scenarios, various hypotheses and parameters were considered based on the ultimate inventory of Brazilian greenhouse gas emissions [12, 25] and the research carried out by D’Avignon [26], as described below.
The slaughter age of animals fed only on pasture is around 36 months4, and is inversely proportional to the increased intensification of the productive system, being reduced to 18 months with confinement or 26 months with semi-confinement.
In Brazil, the average time of confinement after weaning is 4 months, though it is possible to expand this to six months, in the driest period, between May and October, while for semi-confinement, the average time can be increased by two months, rising to three months.
It is estimated that biogas with 60% methane is generated because of animal waste per day (4.05 m3/animal/day). This value was calculated based on the methane information emitted per head of cattle, in accordance with the latest national inventory of greenhouse gas emissions [12].
For the generation of biogas from the effluents produced during animal slaughter, the value of 7,4872 m3/head slaughtered5 was estimated by D’Avignon [26], for a large part of abattoirs existing in the country in 2010, responsible for approximately 90.8% of cattle slaughtered in the country. In this research the need for electric energy in the productive process was estimated at 39 kWh per head of cattle slaughtered.
Biogas can thus be generated by two distinct sources: the waste produced by the animal and the effluents generated during animal slaughter. However, with the use of bio-digesters, neither are altered in relation to enteric fermentation, irrespective of changes in the productive system. Intensive and extensive systems emit methane, but with a difference in the number of days. The lower the number of days, the lower the emission of methane due to enteric fermentation. Considering all parameters and hypothesis, biogas production potential is given by the following equation:
where:
gbiogas = biogas production potential, in MW.
FC(m3) = conversion factor of m3 to MWh, in which case the value is 0,00043909.
Nconf = number of confined cattle heads.
qent_fer = amount of biogas produced through manure management. In the article, the authors propose 4,05 m3/animal/day.
number of days = number of days with confined cattle. For example, cattle confined for 4 months, total days of confinement, 120 days.
Nab = number of slaughtered cattle in the year.
qabat = amount of biogas produced through abatement. In the study, this value is 7,4872 m3/slaughtered head.
If the biogas is applied to the generation of electricity, there is an increase in the abatement of emissions through the reduction of the use of non-renewable sources and many GHG emitters, normally used by a large part of the agricultural sector. This potential reduction also should be calculated in relation to the grid. The use is proposed of the emission factors calculated by MCTI [27] and applied in the inventories existing for the 2006–2015 period. To analyze the years between 2000 and 2005, the factor observed in 2006 was applied, and for future scenarios the authors proposed the use of the MCTI study [25] which calculated the factors for 2020, 2025, and 2030, while for 2015–2020, 2020–2025, and 2025–2030, the calculation will be based on the linear tendency in each period, measuring the annual rates of emission factors in the initial and end years. From this the emission factor results are obtained for the national grid, as described in Table 2.
Factor emission – national grid - tCO2/MWh | |||||||||||||
0.0323 | 0.0293 | 0.0484 | 0.0246 | 0.0512 | 0.0292 | 0.0653 | 0.0960 | 0.1355 | 0.1244 | 0.0960 | 0.0740 | 0.0571 | |
0.0441 | 0.0340 | 0.0421 | 0.0521 | 0.0646 | 0.0799 | 0.0990 | 0.1106 | 0.1235 | 0.1379 | 0.1540 | 0.1720 |
In the case of projections for electricity demands, it is proposed to use the average rate calculated by EPE [4] with a growth in energy sources projected until 2050 for the agricultural sector. According to EPE [28], per year, the electricity consumption of the agricultural sector will be almost 47,101,500 MWh6. This is equivalent to an increase of around 42.95% in electricity consumption in the 2015–2030 period. Between 2015 and 2030, the annual rate of growth in the consumption of electricity is estimated at 3.81%. These estimations are important to measure how biogas can contribute to meet the growing demand for electricity in the agricultural sector.
However, the use of biogas produces GHG emissions, as indicated by Gómez et al. [28]. According to IPCC, the burning of biogas results in CH4 and N2O emissions, which are considered by subtracting them from the reduction. The standard values supplied by Gómez et al. [13] of 1 and 0.1 kg GHG/TJ biogas for methane and nitrous oxide, respectively, were used. These values will be considered in the final calculation of total emissions in each of the scenarios proposed in the article.
In the case of emissions avoided using biogas, the result is the difference between the emissions generated during the beef production process, irrespective of the system, discounting the emissions avoided using biogas as electricity. Then, emissions with the use of biogas are given by the following:
where:
Ebiogas = biogas burning emission, in tCO2e,
gbiogas = biogas generation potential, in MW.
FECH4 = methane emission factor with the use of biogas (1 kg/TJ).
FC(TJ) = conversion factor from TJ to MWh (0,003599712).
FEN2O = nitrous oxide emission factor with the use of biogas (0,10 kg/TJ).
For emissions abated with biogas, the equation is represented by:
where:
Eabatbiogas = emission abated using biogas in tCO2e,
gbiogas = biogas generation potential, in MW.
fgrid = grid emission factor, in tCO2/MWh (see Table 2).
So, the final abated emissions (EFbiogas) using biogas are given in the formula:
To analyze the impact of increased confinement times and rates due to the use of biogas for the generation of energy for the agricultural sector and the reduction in GHG emissions, three different intensification scenarios are proposed, in addition to the reference one. One with the increased confinement time, another with the elevated confinement rate, and finally joining all the scenarios for the intensification of livestock raising through confinement.
In this scenario, from 2016 until 2030, an average growth rate equal to all the variables seen in the 2000–2015 period is proposed.
In the reference scenario, confinement rates will follow the linear growth tendency observed between 2000 and 2015. In other words, semi-confined cattle will grow at an average annual rate of 0.35%, equal to the growth observed in the period, 9.7%, and confined cattle at an average annual rate of 4,9%, with a growth of around 51.4% occurring during the period. At the same time, the same hypothesis of linear growth of confinement will be applied to both the size of the beef herd and the number of animals slaughtered in the country. In the case of the beef herd, during this period there was an increase of around 1.6% per year (21.4% in the period as a whole) and in the number of slaughtered animals a little less than 1% per year (13.4% between 2000 and 2015).
As Table 3 shows, the number of heads of cattle confined will rise from a little over four million, in 2015, to just over 8.2 million in 2030. By 2030, the number of semi-confined heads of cattle will be a little over 2.8 million, while in 2015, there were around 2.7 million. If we compare the number of heads with some type of confinement in relation to the total heads of the beef herd, the more intensive beef production system rises from almost 3.5%, in 2015, to 4.5%, in 2030. Finally, the total cattle herd, without dairy cows, will rise from almost 193.5 million, in 2015, to a little more than 246.2 million in 2030.
Baseline scenario/Scenario 1 | |||||||
---|---|---|---|---|---|---|---|
2000 | 2005 | 2010 | 2015 | 2020 | 2025 | 2030 | |
Confinement (cattle heads) | 1,950,000 | 2,305,000 | 2,756,201 | 4,008,764 | 5,097,248 | 6,481,284 | 8,241,122 |
Semi-confinement (cattle heads) | 2,440,000 | 2,481,000 | 2,583,042 | 2,702,774 | 2,750,324 | 2,798,710 | 2,847,948 |
Cattle herd (cattle heads) | 151,990,505 | 186,530,771 | 186,616,195 | 193,448,415 | 209,643,506 | 227,194,417 | 246,214,652 |
Abatement (cattle heads) | 35,550,700 | 44,319,921 | 40,848,429 | 41,033,569 | 43,043,047 | 45,150,932 | 47,362,043 |
Energy demand (MWh) | 1,386,477.3 | 1,728,476.9 | 1,593,088.7 | 1,600,309.2 | 1,678,678.8 | 1,760,886.3 | 1,847,119.7 |
In relation to slaughter, the values range from a little above 41 million head of cattle in 2015, to around 47.4 in 2030. The demand for energy for the beef slaughter process will rise from 1600.3 GWh to around 1847.1 GWh (Table 3).
From 2016 until 2030, in relation to the reference scenario, in scenario 1 confinement time increases from 4 to 6 months, and from 2 to 3 months for semi-confinement. The only change in relation to the reference scenario is the confinement time.
The increase in confinement does not result in changes to the initial values calculated for the reference scenario. For this reason, the values of numbers of heads of confined, semi-confined, slaughtered, and total cattle, demand for energy remain the same, as described in Table 3. This parameter will alter the potential of the generation of biogas, as will be observed in the results, and consequently the emissions presented reduced somewhat the total emissions generated by animal slaughter over the years, with these small changes in emissions being observed in relation to the reference scenario in the results present in the next item.
In the period 2016–2030, in scenario 2 there is an increase in the rate of cattle with some type of confinement from the rate seen in 2000–2015, rising from 3.5% to 10.9% by 2030, an annual rise of around 9.7%. This hypothesis will allow the rate of cattle with some type of confinement to reach by 2050 the minimum rate practiced in some countries, such as Australia, or as seen in the state of Texas in the US, of approximately 50% in relation to the total beef herd.
With this, as shows, the value of confined and semi-confined cattle will be altered, and the others maintained in accordance with the calculations made for the reference scenario. Since the confinement rate does not indicate the type of confinement, the hypothesis used to calculate the number of confined and semi-confined cattle from 2015 onwards was the average rate of confined and semi-confined cattle in relation to the total cattle with some type of confinement between 2012 and 2015. The reason for this hypothesis is the tendency for these rates to stabilize in recent years, with little variation, as seen in Figure 4.
Rate of number of confined cattle on total cattle with some type of confinement and number of semi-confined cattle on total cattle with some confinement in Brazil, % (2000–2015). Source: Prepared by the authors.
Between 2012 and 2015, the average of this rate for confined cattle was around 59.4%, with an oscillation lower than 2%, also seen in semi-confined cattle. For semi-confined cattle, this average rate was 40.6%. Therefore, the number of confined cattle will rise from a little over four million in 2015 to around 15.9 million in 2030. Semi-confined cattle will increase from almost 2.7 million (2015) to almost 10.9 million. Total cattle with some type of confinement in 2030 will be around 26.8 million, representing an increase of 74.2% in relation to 2015.
Joining scenarios 1 and 2 creates scenario 3, thus, as has been seen in the previous scenarios, only the basic values for the calculation of the creation of biogas and emissions are altered. Permitted in this scenario is the analysis of all the proposed possibilities for the intensification of livestock raising through the confinement of cattle, and through the increase in both confinement time and the total rate of cattle in some form of confinement.
After the analysis of each of the proposed scenarios and how the basic variables are modified in the three scenarios, the biogas generation potential and to what extent this can meet the demand for energy of slaughterhouses and abattoirs, and the agricultural sector, is examined, as well as the impact on emissions of the insertion of biogas in the energy matrix. In short, it shows how cattle confinement can contribute to energy policy as a complementary renewable source capable of guaranteeing energy security from the point of view of supply without harming the environment.
There is a high potential for the use of biogas. Even without any intensification policy it will be possible to meet almost 80% of the energy demands of abattoirs and slaughterhouses in 2015. In 2030, not only will it be possible to meet all energy demands necessary for animal slaughterhouses, but also energy can be offered to the Brazilian electricity system with an addition of around 371.1 GW/h year. In the case of the agricultural sector, first, it is estimated that the demand for electricity of slaughterhouses and abattoirs will represent almost 6% of the total electricity consumed by the sector in 2015. By 2030 it is estimated that this value will be approximately 4%. The insertion of biogas as a source of electricity can be noted that renewable resources can meet approximately 14.9% of the electricity demands of the agricultural sector in 2030, in a scenario with both an increase in the confinement rate and an expansion of time spent in confinement (Scenario 3). The importance of the use of biogas as an alternative energy source is so relevant that without any alteration in the country’s beef productive system in 2015 biogas could generate sufficient electricity to meet about 4.8% of the demands of the agricultural sector, and in 2030 this value would be practically the same, a little more than 4.7% (Figure 5). Changes in confinement time or in the confinement rate can increase the offer of biogas and can increase even more the additional offer to the grid with an addition of almost 1402.4 GW/h (scenario 1), 2867.1 GW/h (scenario 2), or 5146.3 GW/h (scenario 3). As Figure 6 shows, it is possible to create more than three times the energy demand necessary for animal slaughter (offer of biogas/energy demand = 378.6%) in 2030.
Relation between the supply of biogas and electricity demand for agricultural sector, for each scenario, % (2015, 2010, 2025 and 2030). Source: Prepared by the authors.
Relation between the supply of biogas and the electricity demand for slaughterhouses, % (2015, 2020, 2025 and 2030). Source: Prepared by the authors.
It can be noted that increased confinement time (scenario 1) creates a lower increase in the offer of biogas for electric energy when compared to the increase in the confinement rate and, above all, that the sum of the different intensification polices results in a significant increase. Thus, this result shows the strategic importance of biogas in Brazil as an energy source capable not only of meeting the demand of slaughterhouses and abattoirs, but also part of the energy demands of the agricultural sector.
An additional positive effect of the use of biogas, in addition to the expansion of energy demands, is on the reduction of GHG emissions. Despite having a low grid emission factor due to the high share of hydroelectricity in the Brazilian energetic matrix, the insertion of a renewable source with the low GHG emissions helps keep the emissions of the Brazilian electricity sector low. In the scenario with the implementation of all intensification policies in the agricultural sector (Scenario 3), it is possible to reduce in the long term (2030) a little more than 1201.56 GgCO2e. Without any policy, this reduction will reach 381.12 GgCO2e, as Figure 7 shows.
Emission reduction with biogas use, for each scenario, in GgCO2e (2015, 2020, 2025 and 2030). Source: Prepared by the authors.
Despite being one of the largest beef producers in the world, Brazil still has low productivity indices in the livestock sector, in which the predominance of extensive system means that the country has a stocking rate of between 1 and 2 heads of cattle per hectare. Given this fact, the country has great potential to intensify the sector with the possibility of the reduction of the demand for electricity in abattoirs and slaughterhouses using biogas and even the agricultural sector as a whole and to reduce somewhat greenhouse gas emissions generated by the sector through the introduction of a new renewable and cleaner energy source.
In the scenarios analyzed, it was perceived that the use of biogas in beef cattle confinement is an interesting complementary energy policy, which fits into the need to diversify renewable energy sources to reduce potential problems in electricity supply, in relation to an energy matrix that is dependent on hydroelectricity to meet the growing demand of the agricultural sector for electricity. In scenario 3, it can be observed that the application of policies to intensify the livestock sector allows a considerable reduction in GHG emissions and to meet the needs of both the agricultural sector and the totality of the energy demand of slaughterhouses and abattoirs, which are close to the herd, facilitating the use of biogas to supply them.
More profound studies, assessing the economic question, such as the costs of expanded confinement and if, from the economic point of view, intensification is attractive, need to carry out to confirm whether, from the technical point of view, increasing beef confinement is an interesting energy policy to increase the offer of electricity and to reinforce the energy security of Brazil. The scenarios show the need to modernize livestock raising through the better use of the waste and the effluents created during the slaughter process and to propose as a short and mid-term energy policy.
Therefore, the conclusion is reached that the use of biogas is important to reduce greenhouse gas emissions, contribute to cleaner energy generation and reduce the energy dependence of the agricultural sector in Brazil. In the face of climate change, this energy resource can help the country to help meet the goals proposed under the Paris Agreement and attract foreign investment in view of the enormous potential to generate energy and reduce emissions.
Starch also known as amylum, is an important food product and biomaterial used world-wide for different purposes. Though traditionally used in the food industry, technological advancement has led to its steady relevance in many other sectors such as health and medicine, textile, paper, fine chemicals, petroleum engineering, agriculture, and construction engineering [1]. It is used in the food industry either as food products or additives for thickening, preservation and quality enhancer in baked foods, confectioneries, pastas, soups and sauces, and mayonnaises. Starch is a polysaccharide of glucose made of two types of α-d-glucan chains, amylose and amylopectin. Starch molecules produced by each plant species have specific structures and compositions (such as length of glucose chains or the amylose/amylopectin ratio), and the protein and fat content of the storage organs may vary significantly. Therefore, starch differs depending on the source. This inherent functional diversity due to the different biological sources enlarges its range of industrial uses [2, 3].
\nThe structural and compositional differences in starches from different sources determine its properties and mode of interactions with other constituents of foods that gives the final product the desired taste and texture. In the food industry, starch can be used as a food additive to control the uniformity, stability and texture of soups and sauces, to resist the gel breakdown during processing and to raise the shelf life of products [2]. Starch is relatively easily extractable and does not require complicated purification processes. It is considered to be available in large quantities in major plant sources such as cereal grains and tubers. These sources are generally considered inexpensive and affordable and serve as raw materials for commercial production [4].
\nStarch from
Corn (A) and potato tuber (B) [
The stability of native starch under different pH values and temperatures varies unfavorably. For instance, native starch granule is insoluble in water at room temperature and extremely resistant to hydrolysis by amylase. Hence native starch has limited functionality. In order to enhance properties and functionality such as solubility, texture, viscosity and thermal stability, which are necessary for the desired product or role in the industry, native starches are modified. The widening vista of application possibilities of starches with different properties has made research in non-conventional starches and other native starches more imperative [2, 6, 7]. Recent studies on the relationship between the structural characteristics and functional properties of starches from different sources have continued to provide important information for optimizing industrial applications.
\nModification has been achieved mostly by physical and chemical means. Enzymic and genetic modifications are biotechnological processes which are increasingly being explored [8]. While physical modification methods seemed simple and cheap, such as superheating, dry heating, osmotic pressure treatment, multiple deep freezing and thawing, instantaneous controlled pressure-drop process, stirring ball milling, vacuum ball milling, pulsed electric fields treatment, corona electrical discharges, etc., chemical modification involves the introduction of new functional moieties into the starch molecule via its hydroxyl groups, resulting in marked change in its physicochemical characteristic. The functional characteristics of chemically modified starch depends on a number of factors including the botanic origin of the native starch, reagent used, concentration of reagent, pH, reaction time, the presence of catalyst, type of substituent, degree of substitution, and the distribution of the substituents in the modified starch molecule. Modification is generally achieved through chemical derivatization, such as etherification, esterification, acetylation, cationization, oxidation, hydrolysis, and cross-linking [7]. This chapter discusses the chemical properties of starch and how they determine its application in the food industry.
\nThe chemical behaviour of starch is dependent on the nature of its constituent compounds. Starch is a homopolysaccharides made up of glucose units. However, the homopolysaccharide are of two types namely: amylose, which is a linear chain consisting of about 500–2000 glucose units, and amylopectin, which is highly branched and consist of over 1,000,000 glucose units. The two types of homopolysaccharides constitute approximately 98–99% of the dry weight of starch [2]. The ratio of the two polysaccharides usually varies depending on the botanical origin of the starch. Botanic source reports that starch chain generally consist of 20% amylose and up to 80% amylopectin by mass. It is believed that starch with up to 80% amylose can exist [7]. Some classification categorize starch containing <15% amylose as ‘waxy’, 20–35% as ‘normal’ and greater ≥40% as ‘high’ amylose starches [9].
\nAmylose and amylopectin have different physiochemical properties which impact on the overall properties of the starch. Hence it is often important to determine the concentration of each individual component of the starch, as well as the overall starch concentration [10]. The physicochemical (e.g., gelatinization and retrogradation) and functional (e.g., solubility, swelling, water absorption, syneresis and rheological behaviour of gels) properties determine the potential uses of starches in the food industry. These properties depend on the molecular and structural composition of amylose and amylopectin, percent composition and arrangement of these two homopolysaccharides in starch granules which often determine the granule size and shape depending on other genetic factors as a result of the particular species of plant [2].
\nIn food products, the functional roles of starch could be as a thickener, binding agent, emulsifier, clouding agent or gelling agent. In the food industry, native starch is usually reprocessed and modified through chemical processes to improve its functionality for the desired purpose. Chemical modification involves the introduction of new functional groups into the starch molecule which produces in a modified starch with markedly altered physicochemical properties. Such modified starch shows profound change in functionality such as solubility, gelatinization, pasting and retrogradation [11].
\nThe chemical reactivity of starch is dependent on the reactivity of the constituent glucose units [11]. The chemical and functional properties achieved following such modification depends largely on the reaction conditions such as modifying reagent(s), concentration of the reactants, reaction time, type of catalyst used, pH, and temperature. The type of substituents, degree of substitution and distribution of substituents in the starch molecule affects the functional properties.
\nAmylose is a linear polymer of α-d-glucose units linked by α-1,4 glycosidic bonds (Figure 2). The linear nature of amylose chain and its percentage content in starch, and the relative molecular arrangement with amylopectin affect the overall functionality of the starch. Hence starch varies greatly in form and functionality between and within botanical species and even from the same plant cultivar grown under different conditions. This variability provides starches of different properties, which can create challenges of raw materials inconsistency during processing [12].
\nChemical structure of amylopectin chain and amylose chain.
Amylopectin is a branched polymer of α-d-glucose units linked by α-1,4 and α-1,6 glycosidic bonds (Figure 2). The α-1,6 glycosidic linkages occurs at the branching point while the linear portions within a branch are linked by α-1,4 glycosidic bonds. In comparison to amylose, amylopectin is a much larger molecule with a higher molecular weight and a heavily branched structure built from about 95% (α-1,4) and 5% (α-1,6) linkages. Amylopectin unit chains are relatively short with a broad distribution profile, compared to amylose molecules. They are typically, 18–25 units long on average [13, 14].
\nPhysical properties are those properties exhibited without any change in chemical characteristics of starch and do not involve the breaking and creation of chemical bonds such as solubility, gelatinization, retrogradation, glass transition, etc. On the other hand, chemical properties changes due to chemical reactions and usually involve the breakage and creation of new bonds. Examples of such chemical processes in starch include hydrolysis, oxidation, esterification and etherification. Research strongly indicates that the molecular weight and branching attributes of starch which play important roles in the shape and size of granules can potentially be used for predicting some of its functionality such as texture, pasting, retrogradation, etc. [12, 15]. Amylose has more proportional relationships with pasting and gel textural properties, while amylopectin which are predominant in regular and waxy corn starches, has higher proportional relationship with firmness.
\nWhen unprocessed or native starch granules which are relatively inert are heated in the presence of adequate water, usually during industrial processes, swelling of the granules occur and the amylose dissolves and diffuses out of the swollen granules which upon cooling forms a homogenous gel phase of amylose-amylopectin. The swollen amylopectin-enriched granules aggregate into gel particles, generating a viscous solution. This two-phase structure, called starch paste, is desirable for many food applications where processed starches are used as thickeners or binders [2, 16].
\nRetrogradation of starch is a phenomenon that occurs when the disordered arrangement of the polymer molecules of gelatinized starch begins to re-align into an ordered structure in the food product [15]. Preventing retrogradation affects the freeze-thaw stability and textural characteristics and helps to elongate the shelf life of the food product. Starch modification through chemical means, such as, hydrolysis and esterification are generally used to produce starches that can withstand retrogradation. Preventing retrogradation of starch is important for starch used in frozen foods because it is accelerated at cold temperatures, producing an opaque, crystallized, coarse texture as a result of the separation of the liquid from the gel or syneresis [17, 18]. Crosslinked oxidized starches have been reported be more stable against retrogradation [15].
\nAmylose linear chain dissolves in water at 120–150°C and is characterized by high thermostability, resistance to amylase, high crystallinity and high susceptibility to retrogradation. Amylopectin, which is the branched chain is however, slow to retrogradation, with crystalline forms appearing only on the outside of the globule and characterized by a significantly lower re-pasting temperature of 40–70°C and an increased susceptibility to amylases activity than amylose. Retrogradation of starch is affected botanical origin of the starch, amylose content, length of the amylopectin chains, density of the paste, paste storage conditions, physical or chemical modifications and the presence of other compounds. Recrystallization of starch applies only to amylose chains, and it occurs most readily at temperatures around 0°C, and also at temperatures above 100°C [8]. Physical modification process such as repeated freezing and thawing of the starch paste aggravate retrogradation. The resulting starch thus produced is resistant starch that exhibit resistance to digestibility by amylase enzymes and can be used as an alternative nutrient source for diabetic patients and as a rate controlling polymer coat in controlled drug delivery systems [8].
\nStarch granules swollen with water are predisposed to fragmentation if exposed to physical severe pressure change. This becomes of major concern where the integrity of the granules is required to maintain viscosity. Shear is the disintegration phenomenon of swollen starch granules or gel. Starch shear arises from the shear stress which builds up during the process of retrogradation and/or gel drying of the gelatinized starch [19]. The stress acting in opposite directions creates a fault-line that causes the material to open up or tear apart. Shearing generally depends on the fluid (gel) viscosity and flow velocity [20]. Starch granules in their raw unswollen forms are not susceptible to damage by shear even in the slurry before cooking. But once cooked or gelatinized, starch granules becomes susceptible to shear, resulting in loss of viscosity and textural stability [19].
\nThe chemical properties of starch are dependent on the reactivity of starch which is a function of the polyhydroxyl functional groups in the constituent glucose monomers. The hydroxyl groups at position C-2, C-3 and C-6 which are free from the glycosidic bond linkages and pyranose ring formation, are usually free for substitution reactions involving either the attached hydrogen or the entire hydroxyl group. While the ▬OH at C-6 is a primary alcoholic hydroxyl group, those at C-2 and C-3 are secondary alcoholic hydroxyl group. Hence starch can undergo hydrolytic cleavage of its chains at the glycosidic bonds; oxidative reaction with the ▬OH or C▬C bond creating carbonyl groups; and other reactions with various functional and multifunctional reagents to produce esterified and etherified starches. Most of the reactions require activation of the hydroxyl of glucose units in acidic or basic media [7].
\nThe reactivity of starch is dependent on the hydroxyl functions of the constituent α-D-glucan polymers (Figure 2). Thus starch is able to undergo the following reactions.
\nHydrolysis is an addition reaction and simply involves the addition of a water molecule across a bond resulting in the cleavage of that bond and formation of the cleavage products, usually with hydroxyl group or alcohol functionality. Hydrolysis of starch can be achieved by chemical or enzymatic process. Chemical process of hydrolysis usually employs heating starch in the presence of water or dilute hydrochloric acid (Figure 3). Hydrolysis is also used to remove fatty substances associated with native starches. Hydrolysis under acidic condition is called roasting, resulting in acid modified starch. Treatment of starch with sodium or potassium hydroxide results in alkaline modified starch. Hot aqueous alkaline solutions can be used, and this improves the reducing value of that starch [21, 22, 23].
\nHydrolysis of α(1 → 4) glycosidic bond.
The products of starch hydrolysis include dextrin or maltodextrin, maltose and glucose. Dextrins are mixtures of polymers of d-glucose units linked by α-(1 → 4) or α-(1 → 6) glycosidic bonds. The percentage of products obtained depends on the conditions used for the reaction such as duration and strength/amount of reagents used. Enzymic hydrolysis uses the enzyme malto-amylase to achieve hydrolysis and this is the process that usually occurs in starch digestion in the gastrointestinal tract [9]. Dextrins are white, yellow, or brown water-soluble powder which yield optically active solutions of low viscosity. Most of them can be detected with iodine solution, giving a red coloration. White and yellow dextrins from starch roasted with little or no acid are called British gum. The properties of dextrinized starch is dependent upon the reaction conditions (moisture, temperature, pH, reaction time) and the products characteristics vary in its content of reducing sugar, cold water solubility, viscosity, color and stability.
\nHydrolytic processes have been used in the food industry to produce starch derivatives with better functional properties and processing applications [2]. Acid and alkali steeping are the two most widely used methods for starch isolation in the food industry, with numerous modifications. Thermo-alkali isolation method known as nixtamalization has been used in Central America since pre-Hispanic times. Acid and alkali isolation processes affect the amylose/amylopectin, protein and lipid content as well as the granule size and shape of the final product [23].
\nThe condensation of an alcohol and carboxylic acid usually under acidic condition, to produce an ester and water, is called esterification [24]. Basically, the reaction is between the carboxylic acid group and the alcohol group with the elimination of a water molecule (Figure 4). When the acid anhydride is used, an alkaline condition is preferred in the reaction.
\nEsterification reaction of carboxylic acids and alcohols.
The reaction is usually reversible and the forward reaction is favoured under low pH and excess of alcohol while the reverse is favoured under high pH. Remover of one of the product during the reaction will also favour the forward reaction.
\nFor starch, the reaction is between the carboxylic acid group (▬COOH) of fatty acids or ▬COCl of fatty acid chlorides and the alcohol group (▬OH) of the glucose units. Esterification is generally used to introduce more lipophilic groups into the starch molecule making it more lipophilic and for producing crosslink starch when polyfunctional compounds or multifunctional or reagents capable of esterification or etherification are used [15]. Esterification weakens the inter-molecular bonding that holds the granules together and hence alter the granule shape and sizes as well as other functional properties of the starch. The degree of substitution (DS) is dependent on the concentration of reagent used, the type of reagent used, the catalyst and the duration of reaction [25].
\nStarch can be acetylated by reacting it with acetic anhydride to produce acetylated starch (Figure 5). The hydroxyl group of the glucose units are esterified with the acetyl groups from the acetic anhydride to give starch with glucose units with acetate function. The DS of the hydroxyl group with acetate group is dependent on the reaction conditions. Acetylated corn starch of DS 0.05, 0.07 and 0.08 have been obtained using 4, 6 and 8% (starch d.w.) acetic anhydride respectively and aqueous sodium hydroxide as catalyst [25].
\nAcetylation of starch with acetic anhydride.
The introduction of the more bulky acetyl group compares with hydroxyl group causes steric hindrance to the alignment of the linear chains. This allows for easy water percolation between chains thus increasing the granule swelling power and solubility resulting in lower gelatinization temperature [25]. The steric hindrance of less polar acetyl group also reduces the amount of inter-molecular hydrogen bond formation, and weakens the granule structure, preventing molecular re-association and realignment required for retrogradation. However, depending on the DS and the interplay between the a weakened granular structure as result of interruption of the inter- and intra-molecular bonds, and reduced bonding with water molecules as a result of the hydrophobicity of the acetyl groups, the viscosity of the final product can be enhanced.
\nAcetylation improves paste clarity and freeze-thaw stability of starch. Starch acetates of low DS are commonly used in the food industry for quality consistency, and as texture and stability enhancers. The Food and Drug Administration (FDA) maximum DS of acetylated starches for food application is 0.1 [19]. Starch acetate of high DS exhibit high degree of hydrophobicity and thermoplasticity and are soluble in organic solvents like chloroform and acetone, and are mostly used in non-food applications [25]. At 0.0275 DS, corn starch exhibit lower paste gelling, which is practically lost at 0.05 DS. Most commercial starch acetates have <0.05 DS [19].
\nAcetylated distarch adipate, is a monosubstituted starch obtained by treating starch with acetic anhydride and adipic anhydride (Figure 6). It has been used since the 1950s due to desire for improved stability of product in cold and freezing weather conditions. It is a good temperature change resistant agent used in foods as a bulking agent, stabilizer and thickener. It improves smoothness and sheen of soups and sauces [19]. The improved freeze-thaw stability of acetylated cross-linked waxy maize starch has led to its use in frozen sauces in vegetables, appetizers and pastries. Hydroxypropylation of cross-linked starch also dramatically improves the stability quality of puddings and frozen sauces [19].
\nEsterification of starch with acetic anhydride and adipic anhydride.
When starch granule is esterified with succinic anhydride, it produces succinyl starch, and the process is commonly referred to as succinylation of starch. Succinylation of starch was earlier achieved in the presence of aqueous pyridine and under reflux at 115°C (Figure 7). However, environmental concerns have led to the development of more green synthetic routes. Thus succinic ester of starch have been prepared by mixing starch with succinic anhydride solution in acetone and refluxing at 110°C for 4 h [25]. Sui et al. [26] was also able to induce a reaction by drop-wise addition of succinic anhydride to a water suspension of starch while maintaining pH at 8.5 by drop-wise addition of sodium hydroxide.
\nSuccinylation reaction of starch.
Succinyl group weakens the inter-molecular bonding of starch polymeric chains in the granules, facilitating swelling, solubilisation and gelatinization at lower temperatures. Paste clarity is enhanced and retrogradation is reduced. However, there may be reduced stability against shear at high temperature and during cooling. Starch succinate is ionic and acts as polyelectrolytes. At low degree of substitution (DS), the succinate makes the starch more hydrophilic and viscos in solution [8, 25]. For its viscosity enhancing effect, succinylated starches could find application in production of non-gelling custard creams, and for its increased hydrophilicity, it could be used for enhancing the juicy/smooth taste of meat and fried products. Starch succinates can also be used in soups, snacks, and frozen/refrigerated food products as thickening or stabilizing agents.
\nEsterification of starch with octenylsuccinic anhydride (OSA) or octenylsuccinic acid in the presence of an alkali yields starch octenylsuccinate (Figure 8), while esterification with dodecyl succinic acid yield starch dodecyl succinate. The octenyl or dodecyl group introduce a reasonable level of lipophilicity to the product making it have dual functionality which can be used in emulsification and flavours encapsulation. OSA treated starches are used to stabilize oil-in-water food emulsions associated with beverage concentrates containing flavor and clouding oils [19]. It helps to protect emulsified and spray dried flavour oils against oxidation during storage. FDA allows a DS of 0.02.
\nEsterification of starch with octenylsuccinic acid anhydride.
Commercial production of acetylated starch dodecyl succinate, di-substituted starch of low dodecyl succinate residue employs acetic anhydride reagent at alkaline pH [15]. An alkali-starch complex forms first, which then interacts with the carboxylic anhydride to form a starch ester with the elimination of carboxylate ion and one molecule of water [15]. Starch succinate offers freeze-thaw stability, high-thickening, low-gelatinization temperature, clarity of paste, good film-forming properties and resistance to retrogradation.
\nInorganic esters also exist, for instance, esters of phosphorous acid (H3PO3) and phosphoric acid (H3PO4). When starch granules are reacted with phosphorylating agents such as phosphoric acid, mono- or di-starch phosphate is formed (Figure 9). The resulting starch has increased stability at high and low temperatures, more resistant against acidic condition, and is applicable as a thickening agent. Orthophosphate and pyrophosphate has been used to achieve phosphorylation of starch under slightly acidic and high temperature conditions [27].
\nPhosphorylation reaction of starch.
Phosphoryl trichloride (Figure 10), sodium tripolyphosphate (Figure 11) and sodium trimetaphosphate (Figure 12) have also been used under higher pH to obtain monostarch phosphate and di-starch phosphate [15, 28]. Phosphorylation reactions produce either monostarch phosphate or distarch phosphate which is a cross-linked derivative. However this depends on the reagents and reaction conditions. Usually, monoesters, rather than diesters, are produced with a higher degree of substitution [8]. Steric hindrance as a result of the introduced phosphate groups inhibits the linearity of amylose or the outer branch of the amylopectin chain where it reacted. This weakens the inter-molecular association and creates chains disaggregation, which leads to better paste clarity [8].
\nPhosphorylation of starch with phosphoryl trichloride.
Phosphorylation of starch with sodium tripolyphosphate.
Phosphorylation of starch with sodium trimetaphosphate.
Distarch phosphate has the phosphate group esterified with two hydroxyl groups of two neighbouring starch polymer chains [29]. The phosphate bridge or cross-linking strengthens the mechanical structure of the starch granules. Phosphate cross-linked starches exhibit stability against high temperature, low pH and shear, and improved firmness of the swollen starch granule as well as improved viscosity and textural characteristic. Distarch phosphate is used as thickener and stabilizer and provides stability against gelling and retrogradation and high resistance to syneresis during storage [8].
\nIn solution, several specie of the phosphate ion can exist and anyone may be responsible for the phosphorylation reaction depending on the reaction conditions. Phosphorylation has been demonstrated to mostly occur at the C-3 and C-6 of the glucose units, and the degree of phosphorylation depends on distribution of the chain length of the starch polymers [30]. Blennow et al. [31] also demonstrated that phosphate groups may play important role in the size distribution of the amylopectin side chains of phosphorylated starches. Some researchers have reported that about 60–70% of total phosphorus of starch monophosphate is located at C-6 while the rest is located at C-3 of anhydroglucose units. Most phosphate groups (88%) are on chain β of amylopectin [9].
\nLanderito and Wang [32] reported that phosphorylated starch prepared by the slurry treatment exhibited a lower gelatinization temperature, a higher peak viscosity, a lesser degree of retrogradation, and improved freeze-thaw stability compared with those prepared by the dry-mixing treatment. They believed that phosphorylation probably occurred in both amylose and amylopectin chains, and the amount and location of incorporated phosphate groups varied with starch types, which may be due to their different amylose and amylopectin contents. Waxy starch was more prone to phosphorylation, followed by common and high-amylose starches. Enzymic phosphorylation of starch has been reported [33]. Extrusion condition of 200°C, sodium tripolyphosphate concentration of ≥1.4 g/100 ml and pH 8.5 have been used to obtain starch phosphate with high degree of substitution [34].
\nGenerally, alcohols (▬OH) groups condenses with one another at high temperatures under acidic conditions to form ethers (Figure 13). The reaction mechanism is through a proton transfer from the catalyst to one of the molecule to form a cation, which loses the proton by extracting the ▬OH of the second molecule to form an ether and water.
\nEtherification reaction.
Etherification of starch is usually done by use of epoxide reagents as depicted in Figures 14 and 15. The epoxides are first reduced to diols through a nucleophilic ring opening of the epoxide (cleaving the C▬O bond under aqueous, acidic or alcoholic condition) before the eventual condensation of one of the ▬OH group with that of starch [24]. Some etherification reactions occur under alkaline condition. Like esterification, etherification helps to mostly introduce lipophilic alkyl groups into the starch chains thereby reducing the hydrophilicity and the degree of inter- and intra-molecular hydrogen bonding [8].
\nEtherification of starch with propylene oxide.
Etherification of starch with ethylene oxide.
This reaction process produces hydroxypropylated starch (HPS), which is a starch ether produce by reaction of starch with propylene epoxide in the presence of an alkaline catalyst (Figure 14). HPS is used for enhancing stability and viscosity of food products. The hydroxypropyl groups introduced into the starch chains affect the inter- and intra-molecular hydrogen bonds, thereby allowing for more ease of displacement of starch chains in the amorphous regions [8]. HPS is more stable to prolonged high temperatures than starch acetate especially at pH 6, and has improves freeze-thaw stability. It is mostly used in refrigerated or frozen foods and in the dairy industry. The FDA allowable DS for HPS is 0.2 [19].
\nHydroxyethylation of starch is performed by reacting starch with epoxyethane or ethylene oxide to produce the starch ether, hydroxyethylated starch (HES) (Figure 15). The health concerns of hydroxyethylated starch are limiting its use in the food industry. However they are mostly used in medicine and pharmaceuticals as plasma volume expander and extracorporeal perfusion fluids [35].
\nThis is an etherification reaction process where starch is reacted with sodium chloroacetate or chloroacetic acid under certain conditions to produce carboxymethylated starch (CMS) (Figure 16). The reaction involves refluxing chloroacetic acetic acid with dry starch (anhydroglucose units) in the presence of sodium hydroxide in a solvent mixture of ethanol/isopropanol (ratio 3:5). Anhydroglucose unit can be obtained from acid hydrolysed starch [36].
\nEtherification of starch with sodium chloroacetate.
Another etherification reaction is cationization of starch in which starch react with electrophiles or electron-withdrawing reagents such as ammonium, amino, imino, sulfonium, or phosphonium groups to produce cationic starches (Figures 17–19), which are important industrial derivatives [15]. Cationic starches are usually prepared under alkaline conditions, and they exhibit higher dispersibility and solubility with better transparency and stability.
\nReaction of starch with aziridine to produce amino-ethylated starches [
Reaction of starch and dialkyl cyanamides to produce aminoalkyl starches [
Etherification of starch with sulfonium salt to produce a sulfonium cationic starch.
Cationic starches containing tertiary amino or quaternary ammonium groups are the most important commercial derivatives, however they are mostly used in the textile and paper industry.
\nFor the production of sulfonium starch, halogenoalkyl sulfonium salts (e.g., 2-chloroethyl-methyl-ethyl sulfonium iodide or any β-halogenoalkyl sulfonium salt), vinyl sulfonium salts and the epoxy alkyl sulfonium can be used (Figure 19). Usually R1 is unsaturated group like alkylene, hydroxyalkylene, aralkylene, cycloalkylene, and phenylene group, while each of R2 and R3 can be alkyl, aryl, aralkyl, cycloalkyl and alkylene sulfonium groups and may also contain ether oxygen linkages and amino groups [37]. Factors such as reagent used and temperature, affect the reaction period which usually takes about 16–20 h.
\nSulfonium starch display positive charge and can be used as thickeners in the form of aqueous dispersions or pastes. These dispersions are made by heating the suitable amount of sulfonium starch and water to a temperature of approximately 93°C. Upon cooling, the resulting dispersion becomes considerably clearer and more resistant to viscosity change compared to the untreated starch. Starch succinate and starch citrates which are obtained through esterification reactions have also been observed to exhibit high cationic properties [8].
\nOxidation of starch with strong oxidizing agents mimics reaction of primary alcohols and diols. Primary alcohol ▬OH functions are oxidized (Figure 20) to its corresponding carbonyls (aldehydes and carboxylic acid), while vicinal diols (Figure 21) are cleaved by strong oxidants like periodic acid into its corresponding carbonyl compounds (aldehyde and/or ketones) [24]. Oxidation of secondary alcohol ▬OH produces ketones (Figure 22). Oxidation may result in breakage of some intra- and inter-molecular bonds and partial depolymerization of the starch chains [38].
\nOxidation reaction of primary hydroxyl groups of alcohols.
Oxidation reaction of vicinal hydroxyl groups of alcohols.
Oxidation reaction of secondary hydroxyl groups of alcohols.
Starches treated with oxidants fall into two broad classes: oxidized and bleached.
\nOxidized starches are starches treated with oxidizing agents like sodium hypochlorite (NaOCl). The oxidizing agent can attack the glycosidic bonds hydrolysing them to alcohol (▬OH) functions or/and C▬C bonds of the glucose unit, oxidizing them to carbonyl functions of aldehydes, ketones and carboxylates (Figure 23). Higher pH favors formation of carboxylate groups over aldehydes and ketones. Some depolymerization usually occurs in the process. Introduction of carboxylate groups provides both steric hindrance and electrostatic repulsion. Oxidation is usually carried out on whole granules and it causes the granule to dissolve, rather than swell and thicken [19]. The reaction can introduce up to 1.1% of carboxyl groups in the granule [39]. Oxidation with chlorine or sodium hypochlorite reduces the tendency of amylose to associate or retrograde. The reaction rate of starch with hypochlorite is remarkably affected by pH, which tend to be higher at about pH 7 but becomes very slow at pH 10 [40]. Oxidized starches are used where intermediate viscosity and soft gels are desired, and where the instability of acid-converted starches is unacceptable [41]. Hence, pastes of oxidized starches have a lower tendency to gel compared to those of thin-boiling (or acid hydrolized) starches of comparable viscosity.
\nOxidation reaction of starch to produce oxidized starch.
Other oxidants such as chlorine, hydrogen peroxide and potassium permanganate, dichromates and chlorochromates, etc. are less commonly used. Oxidized starches are reported to give batters improved adhesion to meat products and are widely used in dough and baked foods [41].
\nBleached starch is obtained from oxidation of starch with lower concentrations of oxidizing agents like hydrogen peroxide, sodium hypochlorite, potassium permanganate or other oxidants used to remove color from naturally occurring pigments. Bleaching is done to improve the whiteness and/or eliminate microbial contamination. Reagent levels of about 0.5% are usually used, and loss of some starch viscosity due to hydrolysis usually occurs.
\nCross-linking of the starch polymer chains with reagents that could form bonds with more than one hydroxyl group of molecule results in cross-linked starch. Such reactions randomly add inter- and intra-molecular bonds at different locations in the starch granule which helps to strengthen and stabilize the polymers in the granule. Such processes may employ hydrolysis, oxidation, esterification, etherification, phosphorylation or combinations of these methods in a sequential or one-mix procedure to achieve the desired product that meets the required physicochemical characteristic of gelatinization, viscosity, retrogradation, and textural properties for food applications. In some instances, multifunctional reagents capable of forming either ether or ester inter-molecular linkages between hydroxyl groups on starch molecules are used. Reactions usually take place at the primary ▬OH group of C-6 and secondary ▬OH of C-2 and C-3 of the glucose units. Epichlorohydrin monosodium phosphate, phosphoryl trichloride, sodium trimetaphosphate, sodium tripolyphosphate, a mixture of adipic and acetic anhydride, and vinyl chloride are the main agents used to cross-link food grade starches [15]. Di-starch phosphate (Figure 12) which is a phosphorylated starch is an example of a crosslinked starch. Acetylated distarch adipate (Figure 6), hydroxypropyl distarch phosphate, hydroxypropyl distarch glycerol are other examples of crosslinked starch [8]. The FDA specify that not more than 0.1, 1 and 0.12% DS (w/w of starch) of phosphoryl chloride, sodium trimetaphosphate and adipic-acetic mixed anhydride, respectively, should be used for food grade starch [19].
\nCross-linked starch exhibit increased resistance to processing conditions such as high or low temperatures and pH. Cross-linking reduces granule rupture, loss of viscosity and the formation of a stringy paste during cooking, providing a starch suitable for canned foods and products. Cross-linked starch shows smaller swelling volume, lower solubility and lower transmittance than native starch [15]. While oxidation may increase retrogradation, crosslinking reduces it. Hence a combination of the two chemical modification methods can be used to get the starch with desired balanced characteristics.
\nAs mentioned in the introductory section, native starches are modified to improve their physicochemical properties due to different reasons. Different approaches have been reported including physical, chemical, enzymatic and genetic approach. But the most widely used is the chemical approach. For instance, since starch must be gelatinized for it to be digestible in human diet and nutrition, and the process of gelatinizing native starches usually takes appreciable amount of time for granule to swell and form paste of gel as obtained in cooking rice and corn flour porridge, it can be modified to reduce gelatinization time by physical methods such as extrusion, spray-drier and drum dryer, which promote fast starch gelatinization to produce pregelatinized starch [42, 43, 44]. Pre-gelatinized starch exhibit reduced gelatinization temperature and time. The modified starches are usually dries to obtain flours and/or pre-gelatinized starches of long-term stability and quick preparation [9]. Pregelatinized starches are partially or totally soluble in cold water and readily form pastes [45]. It absorbs more water and disperses readily in water than the untreated starch, forming gel at room temperature and less prone to deposit [46]. Using gelatinized starch in food products affects the food qualities and properties, such as, bread volume and crumb [47]; pastas elasticity and softness, lusciousness and digestibility, tolerance in the properties of beating and cake mixtures, ice creams, doughnuts, growth of sugar crystals in food products [48]; texture, volume, shelf-live and stability during thawing of cakes and breads [49]. Liquefaction, partial hydrolysis and dextrinization may occur during pregelatinization depending on the processing conditions [42, 43, 44].
\nThe process of physical modification does not involved any chemical reaction of starch with a modifying reagent and is referred to as physical modification of starch and the products are known as physically modified starches. However, most modifications of starches are performed through chemical processes. The chemical reactions of starch (hydrolysis, esterification, etherification, oxidation and cationization) are generally exploited in the industry to produce converted or modified starches fit for different purposes in the industry.
\nAccording to the Food and Nutrition Program (FNP) of the FAO [50], a modified starch is a food starch which has one or more of its original physicochemical characteristics altered by treatment in accordance with good manufacturing practice by one of the reaction procedures such as hydrolysis, esterification, etherification, oxidation and cross-linking. For starches subjected to heating in the presence of acid or with alkali, the alteration (mainly hydrolysis) is considered a minor fragmentation. Bleaching is also essentially a process resulting in the colour change only. However, oxidation involves the deliberate creation of carboxyl groups. Treatment of starch with substituting reagents such as orthophosphoric acid etc., results in partial substitution in the 2-, 3- or 6-position of the anhydroglucose unit (AGU) unless the 6-position is occupied for branching in amylopectin chain. For cross-linked starch, where polyfunctional substituting agent, such as phosphorus oxychloride, connects two chains, the structure can be represented by Starch▬O▬R▬O▬Starch, where R is the cross-linking group and Starch refers to the linear and/or branched structure [50].
\nEvolving biotechnological innovations are progressing with enzymatic and genetic modification of starch as a greener alternative to chemical modification due to environmental concerns. Enzymatic modifications basically employ hydrolytic enzymes found in certain bacteria. For instance amylomaltases or α-1,4-α-1,4-glucosyl transferases from
The reactions of starch explained above are exploited to create different types of modified or converted starched to obtain starches with appropriate physicochemical characteristics such as gelatinization, retrogradation, heat stability, solubility, transmittance, colour, texture, etc., for different industrial applications. The food industry is very mindful of safety of chemical residues hence not all types of modified starched are used in foods. Generally, modified starches are used for adhesion and as binder in battered and breaded foods, formed meat and snack seasonings; as dustings for chewing gum and products produced in the bakery; as crisping cover for fried snacks; fat replacer and juiciness enhancement in ice cream and salad dressings; flavour encapsulating agents in beverage clouds; emulsion stabilizers in beverages, creamers and canned foods; foam stabilizer in marshmallows; gelling agents in gum drops and jelly gum; and as expanders in baked snacks and cereal meals [19]. Table 1 gives a summary of the chemical modification processes and their food application.
\nChemical process | \nSpecific treatment | \nProducts | \nFunction | \nFood application | \nReferences | \n
---|---|---|---|---|---|
Hydrolysis | \nAcid treatment | \nAcid-hydrolized starch, acid thinned or thin-boiling and fluidity starches | \nReduced hot-paste viscosity, improved gelling or gel strength. Enhanced textural properties | \nGum, pastilles, jellies | \n[19] | \n
Acid treatment | \nDextrinized starch | \nIncreased solubility and gel stability, reduced viscosity and improved emulsification properties. Encapsulate volatiles aromatic compound such as limonene, isoamyl acetate, ethyl hexanoate and β-ionones | \nFat replacer in bakery and dairy products, bakery glazes, protective coating in confectionery. Flavour encapsulator in seasonings | \n[1, 19] | \n|
NaOH or KOH treatment | \nAlkaline hydrolysed starch | \nIncrease viscosity | \n\n | [22] | \n|
Oxidation | \nSodium hypochlorite oxidized starch | \nOxidized starch | \nLower viscosity, improved whitening of granules, high paste clarity, low temperature stability, and increased adhesion. Reduces retrogradation of cooked starch pastes | \nAs binder in battered meat and breading, film former and binder in confectionery, crispy coating in various fried food stuffs, texturizer in dairy products | \n[15] | \n
Esterification | \n\n | Monosubstituted starch (starch acetates, starch hydroxypropyl ethers, starch monophosphate esters) | \nFreeze-thaw stability, improved emulsification properties | \nAs emulsion stabilizers and for flavor encapsulation in refrigerated and frozen foods | \n[15, 19] | \n
Acetylation with acetic acid anhydride | \nStarch acetate | \nIncreased lipophilicity emulsion stabilizer. Improves quality of any fat/oil-containing products. Reduces rancidity by preventing oxidation. Increase viscosity | \nBulking agent in snack foods, stabilizer and thickener in most foods, improves smoothness and sheen of soups and sauces. Cholesterol-free salad dressings, and flavor encapsulating agents in clouding agents, creamer and beverage. Substitute to gum arabic, egg yolk and caseinates | \n[1, 15, 19] | \n|
Succinylation with succinic acid anhydride | \nStarch succinate | \nImproved viscosity and juice taste. Freeze-thaw stability | \nSoups, snacks, and frozen/refrigerated food products. As thickener and in non-gelling custard creams. Meat and fried products to improve juicy or smooth taste and retain flavour | \n[25] | \n|
\n | \nSuccinylation with OSA | \nOSA starch | \nIncreased paste viscosity, emulsion stabilizer and lower gelatinization temperature. Reduces glycemic response after consumption of beverages | \nBeverage emulsion stabilizers, and mayonnaises. Flavour encapsulating agent for battered meat and meat products | \n[19, 25, 53, 54] | \n
Treatment with adipic anhydride | \nStarch adipate | \nHigher paste viscosity, clarity and stability | \nThickening agent in foods | \n[25] | \n|
Phosphorylation | \nStarch phosphate | \nBetter paste clarity, lower gelatinization temperature, higher viscosity, reduced retrogradation, and improved freeze-thaw stability | \nFrozen foods | \n[8] | \n|
\n | Distarch phosphate | \nStability against high temperature, low pH and shear, and improved firmness of the swollen starch granule as well as improved viscosity and textural characteristic, resistance to syneresis during storage | \nAs a thickener and stabilizer in foods such as soups and sauces | \n[8] | \n|
Etherification | \n\n | Etherified starches | \nImproved clarity of starch paste, greater viscosity, reduced syneresis and freeze-thaw stability | \nAs stabilizer in wide range of food applications such as gravies, dips, sauces, fruit pie fillings and puddings. Flavour encapsulating agent in beverages clouds | \n[15] | \n
Carboxymethylation | \nCMS | \nCold-water solubility | \nCandy foods, sweets | \n[1] | \n|
Hydroxypropylation | \nHPS | \nImproves freeze-thaw stability, water-holding properties, lowers the swelling/pasting temperature, increases paste clarity and reduces gel formation. More stable to prolong high temperatures. Increase solubility | \nSalad dressing, ice creams, refrigerated and frozen foods, and dairy products | \n[19] | \n|
Cationization | \nSulfonium starch | \nHigher dispersibility and solubility with better paste clarity and stability | \n\n | [19] | \n|
Crosslinking | \n\n | Crosslinked starches | \nHigher stability to granules swelling, high temperature, high shear and low pH. Better viscosity and freeze-thaw stability. Volume expander. Delays retrogradation and reduce paste clarity | \nAs thickener and texturizers in soups, sauces, gravies, bakery and dairy products. Filling in fruit pies and canned foods. In bread and dough products as expander and to improve rheological properties | \n[9, 15, 53, 55] | \n
\n | Crosslinked-hydroxypropylated starch | \nA smooth, viscous, clear thickener and freeze-thaw stability | \nGravies, dips, sauces, fruit fillings and puddings | \n[15] | \n|
Pre-gelatinized starch | \n\n | \n | Cold-water solubility and thickening | \nInstant soups, sauces, dressing, desserts and bakery mixes. Thickener in food that receive minimal heat processing such as pastas | \n[15, 19] | \n
Application of chemically modification starches in foods.
Baked products like biscuits, pies, bread, cakes wafers and sausages are high density products requiring heat resistant starches. Hence crosslinked starches are used since they are more resistant to oven baking temperatures of 120 ≥ 230°C. Gelatinized starches are also used in ready-to-eat cereal meals such as corn-flakes, etc. The temperature, humidity and degree of stirring determine the texture and quality of the product.
\nOxidized starches have high clarity or transmittance, low viscosity and low temperature stability. It is frequently used in confectioneries for coating candies and sweets since they easily melt.
\nEtherified and crosslinked starches are mostly used. Crosslinked starched have higher stability for granules-swelling, high temperature resistant, high shear stability and acidic conditions stability. They are used as viscosifiers and texturizers in soups, sauces, gravies, bakery and dairy products. Etherified starches have improved clarity of starch paste, greater viscosity, reduced syneresis and freeze-thaw stability. Crosslinked starches are used in wide range of food applications such as gravies, dips, sauces, fruit pie fillings and puddings.
\nHydrolyzed and esterified starches are mostly used in salad dressing and beverages. Hydrolyzed starch (acid-modified starches) has lower paste viscosity under cold and hot conditions. Hence they are used in mayonnaises and salad dressing [19]. Esterified starches have lower gelatinization temperature and retrogradation, lower tendency to form gels and higher paste clarity, and are used in refrigerated and frozen foods, as emulsion stabilizers and for encapsulation of beverage clouds. OSA starch is used as emulsifiers in mayonnaises and salad dressings.
\nPregelatinized and crosslinked starches are mostly used in pastas. Gelatinized starch affects pastas elasticity and softness, delectableness and digestibility. Crosslinking gives the needed structural firmness to the pasta.
\nPregelatinized starches are used in puddings, instant lactic mixtures and breakfast foods to achieve thickening or water retention without employing heat. They are also used in ready-to-use bread mixtures. They are used where little or no heat is required and the increased absorption and retention of water improves the quality of the product; as an agglutinant in the meat industry; and as a filling for fruit pies [9, 49].
\nThe importance of starch as a biopolymer continues to be on the upward trend due to its versatility. It has transformed from its traditional use as energy-source food to more sophisticated food and non-food applications. Its growing relevance in modern technological application is as a result of its susceptibility to modification, which transforms the native properties into more desirable and malleable characteristics fit for different purposes. These modifications are only possible due to the chemical reactivity of the constituent glucose monomers of the starch chains. Though the starch granule is inherently almost unreactive, it is however easily activated for reaction by certain conditions such as high or low pH, higher temperature, presence of a catalyst, etc. Under the right condition, starch molecules can undergo hydrolysis, oxidation, esterification and etherification reactions to produced products of improved organoleptic, textural, mechanical and thermoplastic properties of desirable foods and non-foods application. Modified starches like starch acetate, starch phosphate, HPS, CMS, sulfonium starches and their crosslinked derivatives are used for various applications in the food industry. However, concerns for chemical residues in these products and environmental considerations for hazardous chemicals used in some of the process, have led to more studies for greener modification processes. Though biotechnology has evolved enzymic and genetic modification processes for production of some modified starches, they are still highly limited and sometimes uneconomical, hence chemical modification remains the most versatile and mostly used.
\nThe author declares no conflict of interest.
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He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRNVJQA4/Profile_Picture_2022-03-07T13:23:04.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals. His research interests include biochemistry, oxidative stress, reactive species, antioxidants, lipid peroxidation, inflammation, reproductive hormones, phenolic compounds, female infertility.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Associate Prof.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. He has many years of experience in research and teaching in various fields of medicine, is an author/co-author of more than 200 scientific publications, 13 patents, 15 medical books/chapters, including Chapter in Book «Metabolomics», IntechOpen, 2020 «Metabolomic Discovery of Microbiota Dysfunction as the Cause of Pathology».",institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"199461",title:"Prof.",name:"Natalia V.",middleName:null,surname:"Beloborodova",slug:"natalia-v.-beloborodova",fullName:"Natalia V. Beloborodova",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/199461/images/system/199461.jpg",biography:'Natalia Vladimirovna Beloborodova was educated at the Pirogov Russian National Research Medical University, with a degree in pediatrics in 1980, a Ph.D. in 1987, and a specialization in Clinical Microbiology from First Moscow State Medical University in 2004. She has been a Professor since 1996. Currently, she is the Head of the Laboratory of Metabolism, a division of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russian Federation. N.V. Beloborodova has many years of clinical experience in the field of intensive care and surgery. She studies infectious complications and sepsis. She initiated a series of interdisciplinary clinical and experimental studies based on the concept of integrating human metabolism and its microbiota. Her scientific achievements are widely known: she is the recipient of the Marie E. Coates Award \\"Best lecturer-scientist\\" Gustafsson Fund, Karolinska Institutes, Stockholm, Sweden, and the International Sepsis Forum Award, Pasteur Institute, Paris, France (2014), etc. Professor N.V. Beloborodova wrote 210 papers, five books, 10 chapters and has edited four books.',institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"354260",title:"Ph.D.",name:"Tércio Elyan",middleName:"Azevedo",surname:"Azevedo Martins",slug:"tercio-elyan-azevedo-martins",fullName:"Tércio Elyan Azevedo Martins",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/354260/images/16241_n.jpg",biography:"Graduated in Pharmacy from the Federal University of Ceará with the modality in Industrial Pharmacy, Specialist in Production and Control of Medicines from the University of São Paulo (USP), Master in Pharmaceuticals and Medicines from the University of São Paulo (USP) and Doctor of Science in the program of Pharmaceuticals and Medicines by the University of São Paulo. Professor at Universidade Paulista (UNIP) in the areas of chemistry, cosmetology and trichology. Assistant Coordinator of the Higher Course in Aesthetic and Cosmetic Technology at Universidade Paulista Campus Chácara Santo Antônio. Experience in the Pharmacy area, with emphasis on Pharmacotechnics, Pharmaceutical Technology, Research and Development of Cosmetics, acting mainly on topics such as cosmetology, antioxidant activity, aesthetics, photoprotection, cyclodextrin and thermal analysis.",institutionString:null,institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"334285",title:"Ph.D. Student",name:"Sameer",middleName:"Kumar",surname:"Jagirdar",slug:"sameer-jagirdar",fullName:"Sameer Jagirdar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334285/images/14691_n.jpg",biography:"I\\'m a graduate student at the center for biosystems science and engineering at the Indian Institute of Science, Bangalore, India. I am interested in studying host-pathogen interactions at the biomaterial interface.",institutionString:null,institution:{name:"Indian Institute of Science Bangalore",country:{name:"India"}}},{id:"329795",title:"Dr.",name:"Mohd Aftab",middleName:"Aftab",surname:"Siddiqui",slug:"mohd-aftab-siddiqui",fullName:"Mohd Aftab Siddiqui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329795/images/15648_n.jpg",biography:"Dr. Mohd Aftab Siddiqui is currently working as Assistant Professor in the Faculty of Pharmacy, Integral University, Lucknow for the last 6 years. He has completed his Doctor in Philosophy (Pharmacology) in 2020 from Integral University, Lucknow. He completed his Bachelor in Pharmacy in 2013 and Master in Pharmacy (Pharmacology) in 2015 from Integral University, Lucknow. He is the gold medalist in Bachelor and Master degree. He qualified GPAT -2013, GPAT -2014, and GPAT 2015. His area of research is Pharmacological screening of herbal drugs/ natural products in liver and cardiac diseases. He has guided many M. Pharm. research projects. He has many national and international publications.",institutionString:"Integral University",institution:null},{id:"255360",title:"Dr.",name:"Usama",middleName:null,surname:"Ahmad",slug:"usama-ahmad",fullName:"Usama Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255360/images/system/255360.png",biography:"Dr. Usama Ahmad holds a specialization in Pharmaceutics from Amity University, Lucknow, India. He received his Ph.D. degree from Integral University. Currently, he’s working as an Assistant Professor of Pharmaceutics in the Faculty of Pharmacy, Integral University. From 2013 to 2014 he worked on a research project funded by SERB-DST, Government of India. He has a rich publication record with more than 32 original articles published in reputed journals, 3 edited books, 5 book chapters, and a number of scientific articles published in ‘Ingredients South Asia Magazine’ and ‘QualPharma Magazine’. He is a member of the American Association for Cancer Research, International Association for the Study of Lung Cancer, and the British Society for Nanomedicine. Dr. Ahmad’s research focus is on the development of nanoformulations to facilitate the delivery of drugs that aim to provide practical solutions to current healthcare problems.",institutionString:"Integral University",institution:{name:"Integral University",country:{name:"India"}}},{id:"30568",title:"Prof.",name:"Madhu",middleName:null,surname:"Khullar",slug:"madhu-khullar",fullName:"Madhu Khullar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/30568/images/system/30568.jpg",biography:"Dr. Madhu Khullar is a Professor of Experimental Medicine and Biotechnology at the Post Graduate Institute of Medical Education and Research, Chandigarh, India. She completed her Post Doctorate in hypertension research at the Henry Ford Hospital, Detroit, USA in 1985. She is an editor and reviewer of several international journals, and a fellow and member of several cardiovascular research societies. Dr. Khullar has a keen research interest in genetics of hypertension, and is currently studying pharmacogenetics of hypertension.",institutionString:"Post Graduate Institute of Medical Education and Research",institution:{name:"Post Graduate Institute of Medical Education and Research",country:{name:"India"}}},{id:"223233",title:"Prof.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/223233/images/system/223233.png",biography:"Xianquan Zhan received his MD and Ph.D. in Preventive Medicine at West China University of Medical Sciences. He received his post-doctoral training in oncology and cancer proteomics at the Central South University, China, and the University of Tennessee Health Science Center (UTHSC), USA. He worked at UTHSC and the Cleveland Clinic in 2001–2012 and achieved the rank of associate professor at UTHSC. Currently, he is a full professor at Central South University and Shandong First Medical University, and an advisor to MS/PhD students and postdoctoral fellows. He is also a fellow of the Royal Society of Medicine and European Association for Predictive Preventive Personalized Medicine (EPMA), a national representative of EPMA, and a member of the American Society of Clinical Oncology (ASCO) and the American Association for the Advancement of Sciences (AAAS). He is also the editor in chief of International Journal of Chronic Diseases & Therapy, an associate editor of EPMA Journal, Frontiers in Endocrinology, and BMC Medical Genomics, and a guest editor of Mass Spectrometry Reviews, Frontiers in Endocrinology, EPMA Journal, and Oxidative Medicine and Cellular Longevity. He has published more than 148 articles, 28 book chapters, 6 books, and 2 US patents in the field of clinical proteomics and biomarkers.",institutionString:"Shandong First Medical University",institution:{name:"Affiliated Hospital of Shandong Academy of Medical Sciences",country:{name:"China"}}},{id:"297507",title:"Dr.",name:"Charles",middleName:"Elias",surname:"Assmann",slug:"charles-assmann",fullName:"Charles Assmann",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/297507/images/system/297507.jpg",biography:"Charles Elias Assmann is a biologist from Federal University of Santa Maria (UFSM, Brazil), who spent some time abroad at the Ludwig-Maximilians-Universität München (LMU, Germany). He has Masters Degree in Biochemistry (UFSM), and is currently a PhD student at Biochemistry at the Department of Biochemistry and Molecular Biology of the UFSM. His areas of expertise include: Biochemistry, Molecular Biology, Enzymology, Genetics and Toxicology. He is currently working on the following subjects: Aluminium toxicity, Neuroinflammation, Oxidative stress and Purinergic system. Since 2011 he has presented more than 80 abstracts in scientific proceedings of national and international meetings. Since 2014, he has published more than 20 peer reviewed papers (including 4 reviews, 3 in Portuguese) and 2 book chapters. He has also been a reviewer of international journals and ad hoc reviewer of scientific committees from Brazilian Universities.",institutionString:"Universidade Federal de Santa Maria",institution:{name:"Universidade Federal de Santa Maria",country:{name:"Brazil"}}},{id:"217850",title:"Dr.",name:"Margarete Dulce",middleName:null,surname:"Bagatini",slug:"margarete-dulce-bagatini",fullName:"Margarete Dulce Bagatini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217850/images/system/217850.jpeg",biography:"Dr. Margarete Dulce Bagatini is an associate professor at the Federal University of Fronteira Sul/Brazil. She has a degree in Pharmacy and a PhD in Biological Sciences: Toxicological Biochemistry. She is a member of the UFFS Research Advisory Committee\nand a member of the Biovitta Research Institute. She is currently:\nthe leader of the research group: Biological and Clinical Studies\nin Human Pathologies, professor of postgraduate program in\nBiochemistry at UFSC and postgraduate program in Science and Food Technology at\nUFFS. She has experience in the area of pharmacy and clinical analysis, acting mainly\non the following topics: oxidative stress, the purinergic system and human pathologies, being a reviewer of several international journals and books.",institutionString:"Universidade Federal da Fronteira Sul",institution:{name:"Universidade Federal da Fronteira Sul",country:{name:"Brazil"}}},{id:"226275",title:"Ph.D.",name:"Metin",middleName:null,surname:"Budak",slug:"metin-budak",fullName:"Metin Budak",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/226275/images/system/226275.jfif",biography:"Metin Budak, MSc, PhD is an Assistant Professor at Trakya University, Faculty of Medicine. He has been Head of the Molecular Research Lab at Prof. Mirko Tos Ear and Hearing Research Center since 2018. His specializations are biophysics, epigenetics, genetics, and methylation mechanisms. He has published around 25 peer-reviewed papers, 2 book chapters, and 28 abstracts. He is a member of the Clinical Research Ethics Committee and Quantification and Consideration Committee of Medicine Faculty. His research area is the role of methylation during gene transcription, chromatin packages DNA within the cell and DNA repair, replication, recombination, and gene transcription. His research focuses on how the cell overcomes chromatin structure and methylation to allow access to the underlying DNA and enable normal cellular function.",institutionString:"Trakya University",institution:{name:"Trakya University",country:{name:"Turkey"}}},{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",slug:"anca-pantea-stoian",fullName:"Anca Pantea Stoian",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",biography:"Anca Pantea Stoian is a specialist in diabetes, nutrition, and metabolic diseases as well as health food hygiene. She also has competency in general ultrasonography.\n\nShe is an associate professor in the Diabetes, Nutrition and Metabolic Diseases Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. She has been chief of the Hygiene Department, Faculty of Dentistry, at the same university since 2019. Her interests include micro and macrovascular complications in diabetes and new therapies. Her research activities focus on nutritional intervention in chronic pathology, as well as cardio-renal-metabolic risk assessment, and diabetes in cancer. She is currently engaged in developing new therapies and technological tools for screening, prevention, and patient education in diabetes. \n\nShe is a member of the European Association for the Study of Diabetes, Cardiometabolic Academy, CEDA, Romanian Society of Diabetes, Nutrition and Metabolic Diseases, Romanian Diabetes Federation, and Association for Renal Metabolic and Nutrition studies. She has authored or co-authored 160 papers in national and international peer-reviewed journals.",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",country:{name:"Romania"}}},{id:"279792",title:"Dr.",name:"João",middleName:null,surname:"Cotas",slug:"joao-cotas",fullName:"João Cotas",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279792/images/system/279792.jpg",biography:"Graduate and master in Biology from the University of Coimbra.\n\nI am a research fellow at the Macroalgae Laboratory Unit, in the MARE-UC – Marine and Environmental Sciences Centre of the University of Coimbra. My principal function is the collection, extraction and purification of macroalgae compounds, chemical and bioactive characterization of the compounds and algae extracts and development of new methodologies in marine biotechnology area. \nI am associated in two projects: one consists on discovery of natural compounds for oncobiology. The other project is the about the natural compounds/products for agricultural area.\n\nPublications:\nCotas, J.; Figueirinha, A.; Pereira, L.; Batista, T. 2018. An analysis of the effects of salinity on Fucus ceranoides (Ochrophyta, Phaeophyceae), in the Mondego River (Portugal). Journal of Oceanology and Limnology. in press. DOI: 10.1007/s00343-019-8111-3",institutionString:"Faculty of Sciences and Technology of University of Coimbra",institution:null},{id:"279788",title:"Dr.",name:"Leonel",middleName:null,surname:"Pereira",slug:"leonel-pereira",fullName:"Leonel Pereira",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279788/images/system/279788.jpg",biography:"Leonel Pereira has an undergraduate degree in Biology, a Ph.D. in Biology (specialty in Cell Biology), and a Habilitation degree in Biosciences (specialization in Biotechnology) from the Faculty of Science and Technology, University of Coimbra, Portugal, where he is currently a professor. In addition to teaching at this university, he is an integrated researcher at the Marine and Environmental Sciences Center (MARE), Portugal. His interests include marine biodiversity (algae), marine biotechnology (algae bioactive compounds), and marine ecology (environmental assessment). Since 2008, he has been the author and editor of the electronic publication MACOI – Portuguese Seaweeds Website (www.seaweeds.uc.pt). He is also a member of the editorial boards of several scientific journals. Dr. Pereira has edited or authored more than 20 books, 100 journal articles, and 45 book chapters. He has given more than 100 lectures and oral communications at various national and international scientific events. He is the coordinator of several national and international research projects. In 1998, he received the Francisco de Holanda Award (Honorable Mention) and, more recently, the Mar Rei D. Carlos award (18th edition). He is also a winner of the 2016 CHOICE Award for an outstanding academic title for his book Edible Seaweeds of the World. In 2020, Dr. Pereira received an Honorable Mention for the Impact of International Publications from the Web of Science",institutionString:"University of Coimbra",institution:{name:"University of Coimbra",country:{name:"Portugal"}}},{id:"61946",title:"Dr.",name:"Carol",middleName:null,surname:"Bernstein",slug:"carol-bernstein",fullName:"Carol Bernstein",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/61946/images/system/61946.jpg",biography:"Carol Bernstein received her PhD in Genetics from the University of California (Davis). She was a faculty member at the University of Arizona College of Medicine for 43 years, retiring in 2011. Her research interests focus on DNA damage and its underlying role in sex, aging and in the early steps of initiation and progression to cancer. In her research, she had used organisms including bacteriophage T4, Neurospora crassa, Schizosaccharomyces pombe and mice, as well as human cells and tissues. She authored or co-authored more than 140 scientific publications, including articles in major peer reviewed journals, book chapters, invited reviews and one book.",institutionString:"University of Arizona",institution:{name:"University of Arizona",country:{name:"United States of America"}}},{id:"182258",title:"Dr.",name:"Ademar",middleName:"Pereira",surname:"Serra",slug:"ademar-serra",fullName:"Ademar Serra",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/182258/images/system/182258.jpeg",biography:"Dr. Serra studied Agronomy on Universidade Federal de Mato Grosso do Sul (UFMS) (2005). He received master degree in Agronomy, Crop Science (Soil fertility and plant nutrition) (2007) by Universidade Federal da Grande Dourados (UFGD), and PhD in agronomy (Soil fertility and plant nutrition) (2011) from Universidade Federal da Grande Dourados / Escola Superior de Agricultura Luiz de Queiroz (UFGD/ESALQ-USP). Dr. Serra is currently working at Brazilian Agricultural Research Corporation (EMBRAPA). His research focus is on mineral nutrition of plants, crop science and soil science. Dr. Serra\\'s current projects are soil organic matter, soil phosphorus fractions, compositional nutrient diagnosis (CND) and isometric log ratio (ilr) transformation in compositional data analysis.",institutionString:"Brazilian Agricultural Research Corporation",institution:{name:"Brazilian Agricultural Research Corporation",country:{name:"Brazil"}}}]}},subseries:{item:{id:"14",type:"subseries",title:"Cell and Molecular Biology",keywords:"Omics (Transcriptomics; Proteomics; Metabolomics), Molecular Biology, Cell Biology, Signal Transduction and Regulation, Cell Growth and Differentiation, Apoptosis, Necroptosis, Ferroptosis, Autophagy, Cell Cycle, Macromolecules and Complexes, Gene Expression",scope:"The Cell and Molecular Biology topic within the IntechOpen Biochemistry Series aims to rapidly publish contributions on all aspects of cell and molecular biology, including aspects related to biochemical and genetic research (not only in humans but all living beings). 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