IntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\\n\\n
By listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
All three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\\n\\n
"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\\n\\n
"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\\n\\n
In conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\\n\\n
“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\\n\\n
We invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\\n\\n
Feel free to share this news on social media and help us mark this memorable moment!
After years of being acknowledged as the world's leading publisher of Open Access books, today, we are proud to announce we’ve successfully launched a portfolio of Open Science journals covering rapidly expanding areas of interdisciplinary research.
\n\n\n\n
IntechOpen was founded by scientists, for scientists, in order to make book publishing accessible around the globe. Over the last two decades, this has driven Open Access (OA) book publishing whilst levelling the playing field for global academics. Through our innovative publishing model and the support of the research community, we have now published over 5,700 Open Access books and are visited online by over three million academics every month. These researchers are increasingly working in broad technology-based subjects, driving multidisciplinary academic endeavours into human health, environment, and technology.
\n\n
By listening to our community, and in order to serve these rapidly growing areas which lie at the core of IntechOpen's expertise, we are launching a portfolio of Open Science journals:
All three journals will publish under an Open Access model and embrace Open Science policies to help support the changing needs of academics in these fast-moving research areas. There will be direct links to preprint servers and data repositories, allowing full reproducibility and rapid dissemination of published papers to help accelerate the pace of research. Each journal has renowned Editors in Chief who will work alongside a global Editorial Board, delivering robust single-blind peer review. Supported by our internal editorial teams, this will ensure our authors will receive a quick, user-friendly, and personalised publishing experience.
\n\n
"By launching our journals portfolio we are introducing new, dedicated homes for interdisciplinary technology-focused researchers to publish their work, whilst embracing Open Science and creating a unique global home for academics to disseminate their work. We are taking a leap toward Open Science continuing and expanding our fundamental commitment to openly sharing scientific research across the world, making it available for the benefit of all." Dr. Sara Uhac, IntechOpen CEO
\n\n
"Our aim is to promote and create better science for a better world by increasing access to information and the latest scientific developments to all scientists, innovators, entrepreneurs and students and give them the opportunity to learn, observe and contribute to knowledge creation. Open Science promotes a swifter path from research to innovation to produce new products and services." Alex Lazinica, IntechOpen founder
\n\n
In conclusion, Natalia Reinic Babic, Head of Journal Publishing and Open Science at IntechOpen adds:
\n\n
“On behalf of the journal team I’d like to thank all our Editors in Chief, Editorial Boards, internal supporting teams, and our scientific community for their continuous support in making this portfolio a reality - we couldn’t have done it without you! With your support in place, we are confident these journals will become as impactful and successful as our book publishing program and bring us closer to a more open (science) future.”
\n\n
We invite you to visit the journals homepage and learn more about the journal’s Editorial Boards, scope and vision as all three journals are now open for submissions.
\n\n
Feel free to share this news on social media and help us mark this memorable moment!
\n\n
\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"1637",leadTitle:null,fullTitle:"Wireless Communications and Networks - Recent Advances",title:"Wireless Communications and Networks",subtitle:"Recent Advances",reviewType:"peer-reviewed",abstract:"This book will provide a comprehensive technical guide covering fundamentals, recent advances and open issues in wireless communications and networks to the readers. The objective of the book is to serve as a valuable reference for students, educators, scientists, faculty members, researchers, engineers and research strategists in these rapidly evolving fields and to encourage them to actively explore these broad, exciting and rapidly evolving research areas.",isbn:null,printIsbn:"978-953-51-0189-5",pdfIsbn:"978-953-51-5612-3",doi:"10.5772/2098",price:159,priceEur:175,priceUsd:205,slug:"wireless-communications-and-networks-recent-advances",numberOfPages:614,isOpenForSubmission:!1,isInWos:1,isInBkci:!1,hash:"fe620edc5a08cfb89ffce416d4bc8792",bookSignature:"Ali Eksim",publishedDate:"March 14th 2012",coverURL:"https://cdn.intechopen.com/books/images_new/1637.jpg",numberOfDownloads:86554,numberOfWosCitations:34,numberOfCrossrefCitations:31,numberOfCrossrefCitationsByBook:1,numberOfDimensionsCitations:49,numberOfDimensionsCitationsByBook:2,hasAltmetrics:1,numberOfTotalCitations:114,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 2nd 2011",dateEndSecondStepPublish:"May 30th 2011",dateEndThirdStepPublish:"October 4th 2011",dateEndFourthStepPublish:"November 3rd 2011",dateEndFifthStepPublish:"March 2nd 2012",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6,7",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"14018",title:"Dr.",name:"Ali",middleName:null,surname:"Eksim",slug:"ali-eksim",fullName:"Ali Eksim",profilePictureURL:"https://mts.intechopen.com/storage/users/14018/images/2462_n.jpg",biography:"Ali EKŞİM was born in 1978, in Denizli, Turkey. He is currently a Division Manager at the Center of Research for Advanced Technologies of Informatics and Information Security (TUBITAK-BILGEM) in Gebze, Kocaeli, Turkey. He received his B.Sc. degree from Yeditepe University, his M.Sc. degree from Koç University, and his Ph.D. degree from Istanbul Technical University, Istanbul, Turkey in 2001, 2004 and 2011, respectively. He has a book, a book chapter, and an editor of Wireless Communications and Networks: Recent Advances. In addition, he has more than 50 research publications in various national and international journals and conference proceedings. His current research interests lie in wireless communications, space–time coding, coding and modulation, physical layer security, wireless sensor networks and digital signal processing.\r\nDr. Ekşim is a Senior Member of the International Association of Computer Science and Information Technology, and Member of International Association of Engineers and Institue of Electrical & Electronics Engineers. He has been given a URSI Young Scientist Awards for the URSI-GASS Symposium, which is to be held in Istanbul, Turkey in 2011.",institutionString:null,position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"1",institution:null}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"530",title:"Computer Network",slug:"communications-and-security-computer-network"}],chapters:[{id:"31606",title:"Latest Progress in MIMO Antennas Design",doi:"10.5772/35887",slug:"latest-progress-in-mimo-antennas-design",totalDownloads:3756,totalCrossrefCites:2,totalDimensionsCites:3,hasAltmetrics:0,abstract:null,signatures:"Yue Li, Jianfeng Zheng and Zhenghe Feng",downloadPdfUrl:"/chapter/pdf-download/31606",previewPdfUrl:"/chapter/pdf-preview/31606",authors:[{id:"106248",title:"Dr.",name:"Yue",surname:"Li",slug:"yue-li",fullName:"Yue Li"},{id:"128854",title:"Dr.",name:"Jianfeng",surname:"Zheng",slug:"jianfeng-zheng",fullName:"Jianfeng Zheng"},{id:"128855",title:"Prof.",name:"Zhenghe",surname:"Feng",slug:"zhenghe-feng",fullName:"Zhenghe Feng"}],corrections:null},{id:"31607",title:"Review of the Wireless Capsule Transmitting and Receiving Antennas",doi:"10.5772/36625",slug:"review-of-the-wireless-capsule-transmitting-and-receiving-antennas",totalDownloads:3246,totalCrossrefCites:11,totalDimensionsCites:17,hasAltmetrics:1,abstract:null,signatures:"Zhao Wang, Eng Gee Lim, Tammam Tillo and Fangzhou Yu",downloadPdfUrl:"/chapter/pdf-download/31607",previewPdfUrl:"/chapter/pdf-preview/31607",authors:[{id:"55552",title:"Dr.",name:"Tammam",surname:"Tillo",slug:"tammam-tillo",fullName:"Tammam Tillo"},{id:"61533",title:"Prof.",name:"Eng Gee",surname:"Lim",slug:"eng-gee-lim",fullName:"Eng Gee Lim"},{id:"109123",title:"Dr.",name:"Zhao",surname:"Wang",slug:"zhao-wang",fullName:"Zhao Wang"},{id:"135719",title:"Mr.",name:"Fang Zhou",surname:"Yu",slug:"fang-zhou-yu",fullName:"Fang Zhou Yu"}],corrections:null},{id:"31608",title:"Travelling Planar Wave Antenna for Wireless Communications",doi:"10.5772/35914",slug:"travelling-planar-wave-antenna-for-wireless-communications",totalDownloads:7179,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:0,abstract:null,signatures:"Onofrio Losito and Vincenzo Dimiccoli",downloadPdfUrl:"/chapter/pdf-download/31608",previewPdfUrl:"/chapter/pdf-preview/31608",authors:[{id:"106334",title:"Dr.",name:"Onofrio",surname:"Losito",slug:"onofrio-losito",fullName:"Onofrio Losito"},{id:"147395",title:"Mr.",name:"Vincenzo",surname:"Dimiccoli",slug:"vincenzo-dimiccoli",fullName:"Vincenzo Dimiccoli"}],corrections:null},{id:"31609",title:"Superstrate Antennas for Wide Bandwidth and High Efficiency for 60 GHz Indoor Communications",doi:"10.5772/34733",slug:"superstrate-antennas-for-wide-bandwidth-and-high-efficiency-for-60-ghz-indoor-communications",totalDownloads:4714,totalCrossrefCites:2,totalDimensionsCites:2,hasAltmetrics:0,abstract:null,signatures:"Hamsakutty Vettikalladi, Olivier Lafond and Mohamed Himdi",downloadPdfUrl:"/chapter/pdf-download/31609",previewPdfUrl:"/chapter/pdf-preview/31609",authors:[{id:"4340",title:"Dr.",name:"Olivier",surname:"Lafond",slug:"olivier-lafond",fullName:"Olivier Lafond"},{id:"101415",title:"Dr.",name:"Hamsakutty",surname:"Vettikalladi",slug:"hamsakutty-vettikalladi",fullName:"Hamsakutty Vettikalladi"},{id:"111050",title:"Prof.",name:"Mohamed",surname:"Himdi",slug:"mohamed-himdi",fullName:"Mohamed Himdi"}],corrections:null},{id:"31610",title:"Hardware Implementation of Wireless Communications Algorithms: A Practical Approach",doi:"10.5772/35181",slug:"hardware-implementation-of-wireless-communications-algorithms",totalDownloads:4405,totalCrossrefCites:0,totalDimensionsCites:1,hasAltmetrics:0,abstract:null,signatures:"Antonio F. 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1. Introduction
The growing demand in manufacturing has led to a significant process of heat energy transfer in industry applications such as nuclear reactors, heat exchangers, radiators in automobiles, solar water heaters, refrigeration units and the electronic cooling devices. Enhancing the heating and cooling processes in industries will save energy, reduce the processing time, enhances thermal rate and increase the equipment’s lifespan. Sivashanmugam [1] found that nanofluid emergence has improved heat transfer capabilities for processes in industries. Choi and Eastman [2] established the nanofluid by synthesizing nanoparticles in the conventional base fluid. To be specific, nanofluid is created by suspending nano-sized particles with commonly less than 100 nm into the ordinary fluids such as ethylene glycol, propylene glycol, water and oils [3]. Various materials from different groups can be used as the nanoparticles such as Al2O3 and CuO from metalic oxide, Cu, Ag, Au from metals, SiC and TiC from carbide ceramics, as well as TiO3 from semiconductors [4]. In addition, immersion of nanoparticles is a new way of enhancing thermal conductivity of ordinary fluids which directly improves their ability in heat transportation [5]. In line with nanofluid’s contribution in many crucial applications, a number of research has been carried out to discover the impacts of various nanofluid suspension on the flow features and heat transfer with several effects including Sulochana et al. [6] considering CuO-water and TiO-water, Sandeep and Reddy [7] using Cu-water, and Abbas and Magdy [8] choosing Al2O3-water as their nanofluid.
Magnetohydrodynamics (MHD) is known as the resultant effect due to mutual interaction of magnetic field and moving electrical conducting fluid. Their great applications such as power generation system, MHD energy conversion, pumps, motors, solar collectors have drawn significant attention of several researcher for MHD nanofluid in convective boundary layer flow [9]. Benos and Sarris [10] studied the impacts of MHD flow of nanofluid in a horizontal cavity. Hussanan et al. [11] analyzed the transportation of mass and heat for MHD nanofluid flow restricted to an accelerated plate in a porous media. In this study, water-based oxide and non-oxide had been considered as the nanofluids. Prasad et al. [12] performed similar work as [11] concerning the radiative flow of nanofluid over a vertical moving plate. Anwar et al. [13] conducted the MHD nanofluid flow in a porous material with heat source/sink and radiation effects. Cao et al. [14] analyzed the heat transfer and flow regimes for a Maxwell nanofluid under MHD effect. While, Ramzan et al. [15] investigated for a radiative Jeffery nanofluid and Khan et al. [16] carried out for a Casson nanofluid with Newtonian heating.
One of the greatest discoveries in material science history is carbon nanotubes (CNTs), which was discovered by a Japanese researcher in the beginning of the 1990s. Since the discovery, due to the unique electronic structural and mechanical characteristics, CNTs are found as valuable nanoparticles, especially in nanotechnology field. CNTs are great conductance which is highly sought in medical applications. They have been used as drug carriers and have benefited cancer therapy treatments [17]. The high thermal conductivity of CNTs has attracted significant attention from many researchers, including Xue [18], Khan et al. [19] and Saba et al. [20]. CNTs are hollow cylinders of carbon atoms in the forms of metals or semiconductors. CNTs are folded tubes of graphene sheet made up of hexagonal carbon rings, and their bundles are formed. CNTs are classified into two types with respectively differ in the graphene cylinder arrangement which are single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs). SWCNTs has one layer [21], while MWCNTs consist of more than one graphene cylinder layers [22]. Khalid et al. [23] studied the characteristics of flow and heat transfer for CNTs nanofluid affected by MHD and porosity effects. Acharya et al. [24] discussed a comparative study on the properties of MWCNTs and SWCNTs suspended in water with the imposition of magnetic field. The CNTs nanofluid flow induced by a moving plate was investigated by Anuar et al. [25] and a prominent effect on heat transfer and skin friction by SWCNTs was observed. Ebaid et al. [26] analyzed convective boundary layer for CNTs nanofluid under magnetic field effect. The closed form solution was derived using Laplace transform method and the findings showed increasing magnetic strength and volume fraction of CNTs had deteriorated the rate of heat transport. Aman et al. [27] improved heat transfer for a Maxwell CNTs nanofluid moving over a vertical state plate with constant wall temperature. The investigation of velocity slip of carbon nanotubes flow with diffusion species was conducted by Hayat et al. [28]. Recently, the heat transmission analysis for water-based CNTs was discussed by Berrehal and Makinde [29], considering the flow over non-parallel plates and Ellahi et al. [30] considering flow past a truncated wavy cone.
Due to its broad range of uses such as car brake system, manufacturing of glass and plastic films, gas turbines, and medical equipment’s, numerous researchers have effectively studied heat transfer and fluid flow in a rotation system [31]. The impact of MHD and porosity on rotating nanofluid flow with double diffusion by using regular nanoparticles was discussed by Krishna and Chamkha [32]. More features of heat transfer affected by porosity and magnetic field for a rotating fluid flow were referred in Das et al. [33] and Krishna et al. [34, 35]. Kumam et al. [36] implemented CNTs in analyzing the flow behavior for a rotating nanofluid. The nanofluid was considered as an electrical conducting fluid moving in a channel under heat source/sink and radiation effects. More study on the heat propagation for a convective flow of nanofluid in a rotating system affected by CNTs with several effects and different geometries were presented by Imtiaz et al. [37], Mosayebidorcheh and Hatami [38] and Acharya et al. [39]. Interestingly, several researchers had recently concentrated their study on the non-coaxial rotation flow. Mixer machines in food processing industry, cooling pad of electronic devices and rotating propellers for aircraft have become great application to exemplify the non-coaxial rotating phenomenon in various industries. Mohamad et al. [40] presented the mathematical expression for heat transfer in non-coaxial rotation of viscous fluid flow. As the extension of the previous study, the heat and mass transfer effects (double diffusion) were considered by Mohamad et al. [41] and followed by Mohamad et al. [42] investigating porosity effect in double diffusion flow of MHD fluid. Ersoy [43] imposed a disk with non-torsional oscillation to study the convective non-coaxial rotating flow for a Newtonian fluid. Mohamad et al. [44, 45] worked on similar study considering the second grade fluid and Rafiq et al. [46] concerning the Casson fluid model. The time dependent flow of an incompressible fluid with MHD, chemical reaction and radiation effects under non-coaxial rotation was investigated by Rana et al. [47]. Subjecting to the same type of rotation, Mohamad et al. [48] studied the porosity and MHD consequences in mixed convection flow influenced by an accelerated disk. The study was improved by Noranuar et al. [49] including the effects of double diffusive flow. According to the review of non-coaxial rotation, it is clear that most of the study are subjected to the ordinary fluid. However, the study of non-coaxial rotation for nanofluid by using regular nanoparticles had been performed by Das et al. [50] and Ashlin and Mahanthesh [51] but then the study reporting the implementation of CNTs in non-coaxial rotation flow remains limited.
Inspiring from the above literature, new study is essential to explore more findings on non-coaxial rotation of CNTs nanofluid. Therefore, the investigation of MHD non-coaxial rotating flow of CNTs nanofluid due to free convection in a porous medium become the primary focus of the current study. Water base fluid is chosen to suspend nanoparticle of SWCNTs and MWCNTs. The exact solutions for velocity and temperature distributions are attained by solving the problem analytically using the Laplace transform method. The results are illustrated in several graphs and tables for further analysis of various embedded parameters.
2. Problem formulation
The incompressible time-dependent carbon nanofluid instigated by non-coaxial rotation past a vertical disk with an impulsive motion is considered as illustrated in Figure 1, where x and z are the Cartesian coordinates with x-axis is chosen as the upward direction and z-axis is the normal of it. The semi-finite space z>0 is occupied by nanofluid that composed by constant kinematic viscosity υnf of SWCNTs and MWCNTs suspended in water and acts as an electrically conducting fluid flowing through a porous medium. The disk is placed vertically along the x-axis with forward motion and a uniform transverse magnetic field of strength B0 is applied orthogonal to it. The plane x=0 is considered as rotation axes for both disk and fluid. Initially, at t=0, the fluid and disk are retained at temperature T∞ and rotate about z′-axis with the same angular velocity Ω. After time t>0, the fluid remains rotating at z′-axis while the disk begins to move with velocity U0 and rotates at z-axis. Both rotations have a uniform angular velocity Ω. The temperature of the disk raises to Tw and the distance between the two axes of rotation is equal to ℓ. With above assumptions, the usual Boussinesq approximation is applied, and the nanofluid model proposed by Tiwari and Das [52] is used to represent the problem in the governing equations, express as
The corresponding initial and boundary conditions are
Fz0=Ωℓ;Tz0=T∞;z>0,
F0t=U0;T0t=Tw;t>0,E3
F∞t=Ωℓ;T∞t=T∞;t>0,
in which F=f+ig is the complex velocity; f and g are (real) primary and (imaginary) secondary velocities respectively, T is the temperature of nanofluid and U0 is the characteristic velocity. The following nanofluid constant for dynamic viscosity μnf, density ρnf, heat capacitance ρCpnf, electrical conductivity σnf, thermal expansion coefficient βTnf and thermal conductivity knf can be used as
where the subscripts f is for fluid and CNTs is for carbon nanotubes. Meanwhile, ϕ is the solid volume fraction of nanofluid. The constants in Eq. (4) are used based on the thermophysical features in Table 1.
Material
Properties
ρKgm−3
CpJKg−1K−1
kWm−1K−1
β×10−5K−1
σSm−1
Water
997.1
4179
0.613
21
0.05
SWCNTs
2600
425
6600
27
106−107
MWCNTs
1600
796
3000
44
1.9×10−4
Table 1.
Thermophysical features of water, SWCNTs, and MWCNTs.
Introducing following dimensionless variables
F∗=FΩℓ−1,z∗=Ωυz,t∗=Ωt,T∗=T−T∞Tw−T∞.E5
Using Eqs. (4) and (5), the governing equations in Eqs. (1)–(3) reduce to (excluding the * notation to simplify the equations)
At this point, d1 and a1 are constant parameters, M is the magnetic parameter (magnetic field), K is the porosity parameter, Pr is Prandtl number, Gr is Grashof number and U is the amplitude of disk. Besides that, the other constant parameters are
Next, the system of equations in Eqs. (6)–(8) after applying Laplace transform yield to the following form
d2dz2F¯zq−ϕ1q+d2F¯zq=−d3GrT¯zq,E11
F¯0q=U−11q,F¯∞q=0,E12
d2dz2T¯zq−a1qT¯zq=0,E13
T¯0q=1q,T¯∞q=0.E14
Then, Eqs. (11) and (13) are solved by using the boundary conditions, Eqs. (12) and (14). After taking some manipulations on the resultant solutions, the following Laplace solutions form
are defined, respectively. The exact solutions for the temperature and velocity are finally generated by utilizing the inverse Laplace transform on Eqs. (15) and (16). Hence, it results
In this study, the skin friction τt and Nusselt number Nu for the flow of Newtonian nanofluid in non-coaxal rotation are also analyzed. Their dimensional form is expressed as
τt=−μnf∂F∂zz=0E22
Nu=−knf∂T∂zz=0E23
Incorporating Eqs. (22) and (23) with the nanofluid model Eq. (4), dimensionless variables Eq. (5) and solutions Eqs. (18) and (19), the following dimensionless skin friction and Nusselt number form as
The dimensionless differential equations of non-coaxial rotating nanofluid flow with associated boundary and initial conditions are analytically solved using the method of Laplace transform to obtain the closed form solutions of heat transfer. Further analysis for the role of dimensionless time t, Grashof number Gr, volume fraction of nanoparticles ϕ, porosity parameter K, magnetic field parameter M and amplitude of disk U on velocity and temperature distributions as well as Nusselt number and skin friction are presented in figures and tables. The profiles are plotted with the physical value of parameters as Pr=6.2,Gr=0.5,M=0.2,K=2.0,ϕ=0.02,U=2.0 and t=0.2. The values are same unless for the investigated parameter of the profile. Since the rotating nanofluid is part of the problem, the results are discussed by presenting the graph of velocity profile in real and imaginary parts, specifically describes the primary f and secondary g velocities. The velocity profiles are demonstrated in Figures 2–7 and the temperature profiles are illustrated in Figures 8 and 9. From these profiles, it is found that all the obtained results satisfy both boundary and initial conditions. SWCNTs and MWCNTs have an identical nature of fluid flow and heat transfer.
Figure 2.
Profile of f and g for varied values of t.
Figure 3.
Profile of f and g for varied values of Gr.
Figure 4.
Profile of f and g for varied values of M.
Figure 5.
Profile of f and g for varied values of K.
Figure 6.
Profile of f and g for varied values of ϕ.
Figure 7.
Profile of f and g for varied values of U.
Figure 8.
Profile of Tzt for varied values of t.
Figure 9.
Profile of Tzt for varied values of ϕ.
Figure 2 depicts the plotting of f and g profiles with varying t values. Overall, the velocity of both SWCNTs and MWCNTs rises over time. As t increases, the buoyancy force becomes more effective and functions as an external source of energy to the flow, causing the velocity of fluid to increase. Figure 3 illustrates the variation of f and g profiles for SWCNTs and MWCNTs cases under the effect of Gr. It is essential to note that Gr is an approximation of the buoyancy force to the viscous force exerting on the flow. Hence, an increase of Gr suggests to the domination of buoyancy force and reduces the viscosity of fluid. Thus, growing Gr leads to an augment of fluid velocity.
On the other hand, Figure 4 discloses the nature of fluid flow in response to M. For both SWCNTs and MWCNTs cases, the figure suggests that amplifying M decreases f and g profiles. This impact is owing to the fact that a greater M value increases the frictional forces acting on the fluid, commonly known as the Lorentz force. Consequently, the fluid encounters substantial resistance along the flow and its velocity decreases. Next, the contribution of K in SWCNTs and MWCNTs nanofluids for both f and g profiles are displayed in Figure 5. It suggests that K value increases linearly with the velocities for both SWCNTs and MWCNTs. Noting that porosity is also greatly affected by the permeability of a medium, where it determines the ability of a medium to enable the fluid to flow through it. Then, the increasing values of K cause the medium to be more permeable and the fluid can easily pass through the medium. Therefore, it increases both f and g profiles.
Figure 6 reveals the consequences of ϕ on f and g profiles in the cases of SWCNTs and MWCNTs. It shows that increasing ϕ values result in the increment of f profiles and fluctuating trend of g profiles. This suggests significant advantages of non-coaxial rotation in CNTs, especially in industrial and medical applications. In line with a general finding, an analysis proceeding in cancer treatment has reported that the CNTs with higher velocity have been used to reach the tumor’s site. Besides, referring to Figure 7, it is noticed that ascending U also has a positive impact on velocity profiles for both CNTs suspensions, where the velocity ascends linearly with the values of U. As U increases, this proposes to the creation of external sources, which are used to enhance the thrust force acting in the fluid flow. Thus, the velocity fluid elevates with increasing U.
Furthermore, the temperature profiles Tzt under the impacts of t and ϕ are displayed graphically in Figures 8 and 9. It reveals that increment of t and ϕ contributes to a rise in nanofluid temperature for both types of CNTs case and followed by the magnification of thermal boundary layer. Physically, the addition of sufficient ϕ of CNTs can improve nanofluid’s thermal conductivity. The more CNTs being inserted, the higher the thermal conductivity, which unsurprisingly improves the ability of fluid to conduct heat. Therefore, a growth of temperature profile is exhibited for increasing ϕ. The comparison of physical behavior for SWCNTs and MWCNTs are clearer when referring to the zooming box of each graph. Overall, Figures 2–7 reveal that the velocity profile of MWCNTs case is more significant compared to the velocity of SWCNTs. This behavior is agreed to the thermophysical features in Table 1, where MWCNTs have low density, which also being a key factor for the increase of velocity profiles. Meanwhile, from Figures 8 and 9, SWCNTs have provided a prominent effect on temperature profiles as it is affected by a high thermal conductivity property.
Tables 2 and 3 show the results of skin friction (τp and τs) and Nusselt number Nu for various parameters on both cases SWCNTs and MWCNTs. According to Table 2, it shows that both τp and τs of SWCNTs and MWCNTs rise when the strength of M higher. These effects cause the surface to produce high friction drag due to the maximization of wall shear stress. On the contrary, as Gr,K and t increase, both suspension of SWCNTs and MWCNTs report a diminution in τp and τs. This shows that augmentation of Gr,K and t have reduced the friction between fluid and surfaces which lead the velocity to increase. Meanwhile, as ϕ and U increase, both suspension of SWCNTs and MWCNTs report a growth of τp and a diminution in τs. From Table 3, it shows that Nu for both CNTs cases decrease as the values of t increase. However, when involving high ϕ, both SWCNTs and MWCNTs have large Nu which also implies to have a great of heat transfer rate. This effect is also directly affected by the reduction of nanofluid heat capacitance as ϕ increases. Overall, for Table 3, it is found that SWCNTs case have high value of Nu compared to MWCNTs, due to its reduction of heat capacitance. This effect also signifies for a better heat transfer process that can be used in several engineering and industrial system.
t
Gr
M
K
ϕ
U
SWCNTs
MWCNTs
τp
τs
τp
τs
0.2
0.5
0.2
2
0.02
2
1.3811
−0.2550
1.3691
−0.2523
0.4
0.5
0.2
2
0.02
2
1.0318
−0.3492
1.0236
−0.3455
0.2
5
0.2
2
0.02
2
0.6276
−0.2705
0.6195
−0.2676
0.2
0.5
3
2
0.02
2
3.2171
−0.1596
3.0871
−0.1620
0.2
0.5
0.2
3
0.02
2
1.3377
−0.2578
1.3252
−0.2552
0.2
0.5
0.2
2
0.12
2
1.6820
−0.3138
1.6016
−0.2966
0.2
0.5
0.2
2
0.02
3
2.8459
−0.5082
2.8214
−0.5030
Table 2.
Values of primary τp and secondary τs skin friction for SWCNTs and MWCNTs.
The significance of bold emphasis used in Table 2 is for the comparison of the effects for varied values of the particular parameters. For each parameter, the changes of skin friction values are compared among the bold values of parameters.
t
ϕ
Nu
SWCNTs
MWCNTs
0.2
0.02
3.6238
3.5818
0.4
0.02
2.5624
2.5327
0.2
0.12
5.4840
5.3185
Table 3.
Values of Nusselt number Nu for SWCNTs and MWCNTs.
The significance of bold emphasis used in Table 3 is for the comparison of the effects for varied values of the particular parameters. For each parameter, the changes of Nusselt number values are compared among the bold values of parameters.
The accuracy of the obtained solution is verified by comparing solution in Eq. (18) with the solution obtained by Mohamad et al. [40] in Eq. (53). The comparison is conducted by letting magnetic parameter and nanoparticle volume fraction M=ϕ=0, and porosity parameter K→∞ in the present solution for both types of CNTs and letting phase angle ω=0 and amplitude of disk oscillation U=2 in the published work. This comparison shows that f and g profiles for both present and previous works are identical to each other as clearly presented in Figures 10 and 11, which thus proves that the accuracy of obtained solution is verified. Meanwhile, another verification is also carried out to verify the validity of present solution by comparing the values of velocity profiles from the present work with the numerical values solved by numerical Gaver-Stehfest algorithm [53, 54]. Tables 4 and 5 observe that the results of f and g profiles from the exact solution in Eq. (18) and the results from numerical solution are in excellent agreement.
Figure 10.
Comparison of f profiles from present results in Eq. (18) with the published work by Mohamad et al. [40] in Eq. (53).
Figure 11.
Comparison of g profiles from present results in Eq. (18) with the published work by Mohamad et al. [40] in Eq. (53).
Comparison of exact and numerical solution of g profiles for SWCNTs and MWCNTs with t=0.2,Gr=0.5,M=0.2,K=2,ϕ=0.02,U=2,Pr=6.2.
6. Summary with conclusion
The unsteady non-coaxial rotation of water-CNTs nanofluid flow in a porous medium with MHD effect is analytically solved for the exact solutions by applying the Laplace transform method. The temperature and velocity profiles with various values of parameter for the immersion of SWCNTs and MWCNTs are plotted graphically and analyzed for their effects. From the discussion, significant findings emerge:
Both primary and secondary velocities for SWCNTs and MWCNTs suspension increase as the values of t,Gr,K, and U increase while decrease as the values of M increase.
The insertion of higher ϕ of SWCNTs and MWCNTs increases the primary velocity profiles while for secondary velocity profiles, fluctuating trend is reported for both cases.
The temperature of nanofluid increases when ϕ and t increase for both SWCNTs and MWCNTs cases.
MWCNTs have higher primary and secondary velocity profiles compared to SWCNTs because of their low-density property
SWCNTs have higher temperature profile than MWCNTs owing to their high thermal conductivity property.
The increasing values of t,Gr and K decrease both primary and secondary skin friction for both types of CNTs while the increase of M gives opposite effect on both skin friction.
Nusselt number for both CNTs cases reduce as t increases and amplify as ϕ increases.
The findings in present work are in accordance to findings in Mohamad et al. [40] and numerical values obtained by numerical Gaver-Stehfest algorithm.
Acknowledgments
The authors would like to acknowledge the Ministry of Higher Education Malaysia and Research Management Centre-UTM, Universiti Teknologi Malaysia (UTM) for the financial support through vote number 17 J98, FRGS/1/2019/STG06/UTM/02/22 and 08G33.
Conflict of interest
The authors declare that they have no conflicts of interest to report regarding the present study.
Nomenclature
βT
Thermal expansion coefficient
Cp
Specific heat
ρ
Density
σ
Electrical conductivity
μ
Dynamic viscosity
gx
Acceleration due to gravity
k
Thermal conductivity
T
Temperature of nanofluid
T∞
Free stream temperature
Tw
Wall temperature
B0
Magnetic field
k1
Permeability
U0
Characteristic of velocity
Nu
Nusselt number
τ
Skin friction
τp
Primary skin friction
τs
Secondary skin friction
F
Complex velocity
f
Primary velocity
g
Secondary velocity
ϕ
Volume fraction nanoparticles
Ω
Angular velocity
t
Time
i
Imaginary unit
Pr
Prandtl number
Gr
Grashof number
K
Porosity
CNTs
Carbon nanotubes
nf
Nanofluid
f
Fluid
\n',keywords:"Nanofluids, Carbon nanotubes, Newtonian fluid, Magnetohydrodynamics, Heat transfer",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/79179.pdf",chapterXML:"https://mts.intechopen.com/source/xml/79179.xml",downloadPdfUrl:"/chapter/pdf-download/79179",previewPdfUrl:"/chapter/pdf-preview/79179",totalDownloads:130,totalViews:0,totalCrossrefCites:1,totalDimensionsCites:1,totalAltmetricsMentions:0,impactScore:0,impactScorePercentile:0,impactScoreQuartile:0,hasAltmetrics:0,dateSubmitted:"September 17th 2021",dateReviewed:"September 24th 2021",datePrePublished:"November 8th 2021",datePublished:"April 20th 2022",dateFinished:"October 29th 2021",readingETA:"0",abstract:"The study analyzed the heat transfer of water-based carbon nanotubes in non-coaxial rotation flow affected by magnetohydrodynamics and porosity. Two types of CNTs have been considered; single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs). Partial differential equations are used to model the problem subjected to the initial and moving boundary conditions. Employing dimensionless variables transformed the system of equations into ordinary differential equations form. The resulting dimensionless equations are analytically solved for the closed form of temperature and velocity distributions. The obtained solutions are expressed in terms of a complementary function error. The impacts of the embedded parameters are graphically plotted in different graphs and are discussed in detail. The Nusselt number and skin friction are also evaluated. The temperature and velocity profiles have been determined to meet the initial and boundary conditions. An augment in the CNTs’ volume fraction increases both temperature and velocity of the nanofluid as well as enhances the rate of heat transport. SWCNTs provides high values of Nusselt number compared to MWCNTs. For verification, a comparison between the present solutions and a past study is conducted and achieved excellent agreement.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/79179",risUrl:"/chapter/ris/79179",book:{id:"10779",slug:"21st-century-nanostructured-materials-physics-chemistry-classification-and-emerging-applications-in-industry-biomedicine-and-agriculture"},signatures:"Wan Nura’in Nabilah Noranuar, Ahmad Qushairi Mohamad, Sharidan Shafie, Ilyas Khan, Mohd Rijal Ilias and Lim Yeou Jiann",authors:[{id:"190576",title:"Dr.",name:"Ilyas",middleName:null,surname:"Khan",fullName:"Ilyas Khan",slug:"ilyas-khan",email:"ilyaskhanqau@yahoo.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Majmaah University",institutionURL:null,country:{name:"Saudi Arabia"}}},{id:"190595",title:"Dr.",name:"Sharidan",middleName:null,surname:"Shafie",fullName:"Sharidan Shafie",slug:"sharidan-shafie",email:"sharidan@utm.my",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"436205",title:"M.Sc.",name:"Wan Nura’in Nabilah",middleName:null,surname:"Noranuar",fullName:"Wan Nura’in Nabilah Noranuar",slug:"wan-nura'in-nabilah-noranuar",email:"wannurainnabilah189@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"436213",title:"Dr.",name:"Ahmad Qushairi",middleName:null,surname:"Mohamad",fullName:"Ahmad Qushairi Mohamad",slug:"ahmad-qushairi-mohamad",email:"ahmadqushairi@utm.my",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null},{id:"436214",title:"Dr.",name:"Mohd Rijal",middleName:null,surname:"Ilias",fullName:"Mohd Rijal Ilias",slug:"mohd-rijal-ilias",email:"mrijal@uitm.edu.my",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:{name:"Universiti Teknologi MARA",institutionURL:null,country:{name:"Malaysia"}}},{id:"436215",title:"Dr.",name:"Lim Yeou",middleName:null,surname:"Jiann",fullName:"Lim Yeou Jiann",slug:"lim-yeou-jiann",email:"jiann8807@gmail.com",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Problem formulation",level:"1"},{id:"sec_3",title:"3. Exact solution",level:"1"},{id:"sec_4",title:"4. Physical quantities",level:"1"},{id:"sec_5",title:"5. Analysis of results",level:"1"},{id:"sec_6",title:"6. Summary with conclusion",level:"1"},{id:"sec_7",title:"Acknowledgments",level:"1"},{id:"sec_10",title:"Conflict of interest",level:"1"},{id:"sec_9",title:"Nomenclature",level:"1"}],chapterReferences:[{id:"B1",body:'Sivashanmugam P. Application of nanofluids in heat transfer, In: S. N. Kazi (Ed.), An Overview of Heat Transfer, INTECH Publications, Croatia, 2012, 411-440.'},{id:"B2",body:'Choi SUS, Eastman JA. Enhancing thermal conductivity of fluids with nanoparticles, United States, 1995.'},{id:"B3",body:'Azhar WA, Vieru D, Fetecau C. Free convection flow of some fractional nanofluids over a moving vertical plate with uniform heat flux and heat source. Physics of Fluids. 2017:29(9):1-13. 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Hall effects on unsteady MHD rotating flow past a periodically accelerated porous plate with slippage. European Journal of Mechanics / B Fluids. 2018:72:135-143. DOI: https://doi.org/10.1016/j.euromechflu.2018.04.010'},{id:"B34",body:'Krishna MV, Ahamad NA, Chamkha AJ. Hall and ion slip effects on unsteady MHD free convective rotating flow through a saturated porous medium over an exponential accelerated plate. Alexandria Engineering Journal. 2020:59(2):565-577. DOI: https://doi.org/10.1016/j.aej.2020.01.043'},{id:"B35",body:'Krishna MV, Ahamad NA, Chamkha AJ. Numerical investigation on unsteady MHD convective rotating flow past an infinite vertical moving porous surface, Ain Shams Engineering Journal. 2020:1-11. DOI: https://doi.org/10.1016/j.asej.2020.10.013'},{id:"B36",body:'Kumam P, Shah Z, Dawar A, Rasheed HU, Islam S. Entropy generation in MHD radiative flow of CNTs Casson nanofluid in rotating channels with heat source/sink, Mathematical Problems in Engineering. 2019:2019(9158093):1-14. DOI: https://doi.org/10.1155/2019/9158093'},{id:"B37",body:'Imtiaz M, Hayat T, Alsaedi A, Ahmad B. Convective flow of carbon nanotubes between rotating stretchable disks with thermal radiation effects. International Journal of Heat and Mass Transfer. 2016:101:948-957. DOI: http://dx.doi.org/10.1016/j.ijheatmasstransfer.2016.05.114'},{id:"B38",body:'Mosayebidorcheh S, Hatami M. Heat transfer analysis in carbon nanotube-water between rotating disks under thermal radiation conditions. Journal of Molecular Liquids. 2017:240:258-267. DOI: http://dx.doi.org/10.1016/j.molliq.2017.05.085'},{id:"B39",body:'Acharya N, Das K, Kundu PK. Rotating flow of carbon nanotube over a stretching surface in the presence of magnetic field: a comparative study. Applied Nanoscience. 2018:8(3):369-378. 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Journal of Nanofluids. 2018:7(6):1172-1186. DOI: https://doi.org/10.1166/jon.2018.1527'},{id:"B51",body:'Ashlin TS, Mahanthesh B. Exact solution of non-coaxial rotating and non-linear convective flow of Cu–Al2O3–H2O hybrid nanofluids over an infinite vertical plate subjected to heat source and radiative heat. Journal of Nanofluids. 2019:8(4):781-794. DOI: https://doi.org/10.1166/jon.2019.1633'},{id:"B52",body:'Tiwari RK, Das MK. Heat transfer augmentation in a two-sided lid-driven differentially heated square cavity utilizing nanofluids. International Journal of Heat and Mass Transfer. 2007:50:2002-2018. DOI: https://doi.org/10.1016/j.ijheatmasstransfer.2006.09.034'},{id:"B53",body:'Stehfest H. Algorithm 368: Numerical inversion of Laplace transforms [D5]. Communications of the ACM. 1970:13(1):47-49. DOI: https://doi.org/10.1145/361953.361969'},{id:"B54",body:'Villinger H. Solving cylindrical geothermal problems using the Gaver-Stehfest inverse Laplace transform. Geophysics. 1985:50(10):1581-1587. DOI: https://doi.org/10.1190/1.1441848'}],footnotes:[],contributors:[{corresp:null,contributorFullName:"Wan Nura’in Nabilah Noranuar",address:null,affiliation:'
Faculty of Science, Department of Mathematical Sciences, Universiti Teknologi Malaysia, Malaysia
Faculty of Science, Department of Mathematical Sciences, Universiti Teknologi Malaysia, Malaysia
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1. Introduction
Antimicrobial resistance (AMR) is one of the most serious global public health threats in this century, which is especially urgent regarding antibiotic resistance in bacteria [1], particularly in Enterobacterales [2]. This phenomenon has arisen globally in both nosocomial and community settings as a consequence of widespread antibiotics’ consumption [3]. Enterobacterales are a large order of different types of bacteria including Escherichia coli that commonly cause infections both in healthcare settings and in communities [4]. To survive the effects of antibiotics, some Enterobacterales can produce enzymes called extended-spectrum β-lactamases (ESBLs) that break down and destroy some commonly used antibiotics, including penicillins and cephalosporins, and make these drugs ineffective for treating infections [4]. Over the last decade, many studies have reported the presence of extended-spectrum β-lactamases (ESBL)-mediated resistance in Gram-negative bacteria causing infections in patients [5, 6, 7, 8, 9]. Infections that can be caused by ESBL-producing bacteria include urinary tract infection (UTI), diarrhea, skin infections, and pneumonia [10]. Possible medications used to treat ESBL infection include carbapenems, which are useful against infections caused by E. coli or Klebsiella pneumoniae bacteria, fosfomycin, β-lactamase inhibitors, non-β-lactam antibiotics, and colistin when other medications have failed to stop the ESBL infection [10]. Unfortunately, the excessive use of antibiotics, in particular β-lactams, leads to the selection of ESBL-producing strains [11]. Because of the emergence and distribution of multidrug-resistant (MDR) E. coli is complicating the treatment of various serious infections [12, 13], the World Health Organization (WHO) has long recognized the need for an improved and coordinated global effort to contain AMR [1]. The burden of AMR, including MDR, varies between the regions; however, low- and middle-income countries share a disproportionate burden due to multitude of factors embedded in the characteristics of the health system, policy, and the practice [14].
In Burkina Faso, there is an emergence of β-lactam-resistant enterobacteria, both in rural and urban areas [9, 15, 16, 17]. Otherwise, carbapenemase-encoding genes are widespread in many parts of the world [18]. According to a previous study, carbapenemase-producing Enterobacterales (CPE) remain one of the most urgent healthcare threats [2]. To this day, the ESBLs and integrons’ genes have been poorly characterized in Burkina Faso, particularly in enteric bacteria in children less than 5 years of age. However, it is imperative that bacterial isolates from underdeveloped regions undergo extensive MDR characterization to inform national strategies designed to halt the continuing spread of these dangerous pathogens [19]. Therefore, the aim of this study was to determine the resistance of diarrheagenic E. coli strains to β-lactams antibiotics and perform the molecular characterization of extended-spectrum β-lactamases (ESBL) and integrons genes among clinical DEC isolated from stools collected in children less than 5 years of age.
2. Methodology
2.1 Study design, area, and sample population
It is a cross-sectional study conducted in two hospitals of Ouagadougou, Burkina Faso (Paul VI and Schiphra), during August 2013 to October 2015 (Figure 1). The Paul VI hospital is located in peripheral area and the Schiphra’s hospital in the city center at the dam edge of Ouagadougou. Many patients from Ouagadougou and its surroundings attend these two healthcare centers because of the good level of healthcare. The study population comprised children below 5 years attending the hospital for treatment.
Figure 1.
Sampling sites in Ouagadougou.
The specimens were collected adhering to a standard protocol from pediatric patients below 5 years of age with acute diarrhea and who were hospitalized or visited the health centers as outpatient. Thus, children who attended the hospitals for treatment and provided assent (from parents) or consent for the study were included in the study. Any child over the age of 5 years was excluded from the study.
2.2 Sample collection and transport
Three hundred and fifteen stool samples were collected in sterile containers and transported to the laboratory of molecular biology, epidemiology, and surveillance of bacteria and viruses transmitted by food, center for research in biological, food, and nutritional sciences at the Joseph KI-ZERBO University of Ouagadougou within 24 h in a cool box at +4°C for immediate analysis.
2.3 Bacterial isolates
Isolation of E. coli was carried out onto eosin methylene blue agar (Liofilchem, Italy), and the plates were incubated at +37°C for 18–24 h. After this stage, the suspected E. coli colonies were selected and streaked onto Mueller-Hinton agar plate (Liofilchem, Italy). Confirmation was carried out by a biochemical microbiology method based on negative urease (Bio-Rad, France), negative citrate (Liofilchem, Italy), positive indole (Bio-Rad, France), positive lactose (Liofilchem, Italy), and positive orthonitrophenyl-β-D-galactopyranoside (ONPG) (bioMerieux, France). E. coli strains isolated were confirmed by API 20E (bioMérieux, France).
The five main pathogroups of E. coli (Enteroaggregative E. coli: EAEC, Enteropathogenic E. coli: EPEC, Enteroinvasive E. coli: EIEC, Enterohemorrhagic E. coli: EHEC, and Enterotoxigenic E. coli: ETEC) were characterized by the 16-plex polymerase chain reaction (PCR) as described by Antikainen et al. [20].
2.4 Antimicrobial susceptibility testing
All identified isolates of E. coli were treated for susceptibility testing against amoxicillin (25 μg), amoxicillin-clavulanic acid (20/10 μg), ceftriaxone (30 μg), cefotaxime (30 μg), cefepime (30 μg), cefixime (10 μg), piperacillin (75 μg), piperacillin-tazobactam (100 + 10 μg), imipenem (10 μg), and aztreonam (30 μg) (Bio-Rad, France) following disk diffusion method on Mueller-Hinton Agar (Liofilchem, Italy). Results were interpreted based on the European Committee of Antimicrobial Susceptibility Testing (EUCAST) guidelines [21]. These isolates, which were not susceptible (either resistant or intermediate) to three or more antibiotics classes, were considered as MDR [22].
2.5 Screening and confirmation of ESBL and integrons producers
A double synergy test was used for ESBL-producing strains testing. This consisted of placing disks (2–3 cm diameter) of ceftriaxone and cefotaxime around an amoxicillin-clavulanic acid disk on the bacterial plate.
For molecular characterization, DNA extraction was performed using heating method [23]. A loopful of bacterial growth from Mueller-Hinton agar (Liofilchem, Italy) plate was suspended in 1 ml of sterilized water. The mixture was boiled for 10 min at +100°C and centrifuged for 10 min at 12000 rpm at +4°C. Supernatant was then collected and used for the PCR reactions as DNA matrices. Multiplex PCR assays were performed for detecting EBLS-encoding genes (blaTEM, 𝑏𝑙𝑎SHV, 𝑏𝑙𝑎OXA, and blaCTX-M) and the presence of the class 1, class 2, and class 3 integrons from the β-lactams-resistant DEC strains. Primers (GeneCust, France) used for these amplifications are described in Table 1.
Genetic resistance supports
Genes
Primers sequence (5′ to 3′)
Product size (bp)
β-Lactams resistance gene
blaTEM
F: ATG AGT ATT CAA CAT TTC CG
1080
R: CCA ATG CTT ATT CAG TGA GG
blaSHV
F: TTA TCT CCC TGT TAG CCA CC
768
R: GAT TTG CTG ATT TCG CTC GG
blaOXA
F: ATG AAA AAC ACA ATA CAT ATC
813
R: AAT TTA GTG TGT TTA GAA TGG
blaCTX-M
F: -ATG TGC AGY ACC AGT AAR GT
544
R: -TGG GTR AAR TAR GTS ACC AGA
Integrons
Int1
F: ATT TCT GTC CTG GCT GGC GA
600
R: ACA TGT GAT GGC GAC GCA CGA
Int2
F: CAC GGA TAT GCG ACA AAA AGG T
806
R: GTA GCA AAC GAC TGA CGA AAT G
Int3
F: GCC CCG GCA GCG ACT TTC AG
600
R: ACG GCT CTG CCA AAC CTG ACT
Table 1.
List of all primers used for antibiotic ESBL genes and integrons detection.
Thermocycling conditions were as follows: 5 min at +94°C, followed by 35 amplification cycles of +94°C for 30 s, 59 ± 4°C for 60 s, and +72°C for 60 s with a final extension of +72°C for 10 min on a thermal cycler (Gene Amp 9700, Applied Biosystems). PCR products were revealed on 1.5% stained Redsaf agarose gel (Prolabo, France), after electrophoresis under UV light (Gel Logic 200).
The PCR assays were carried out in a 25-ml reaction mixture, which consisted of 2.5 μl of the supernatant added to 22.5 μl of reaction mixture. This mixture contained 5 U of Taq DNA polymerase (Accu Power, South Korea), deoxyribonucleic triphosphate (10 mM), buffer GC (10X), MgCl2 (25 mM), and PCR primers (10 μM). Thermocycling conditions were as follows: 5 min at +94°C, followed by 35 amplification cycles at +94°C for 30 s, + 59 ± 4°C for 60 s, and +72°C for 60 s with a final extension of +72°C for 10 min on a thermal cycler (AB Applied Biosystems). Following PCR, the reaction products were separated using electrophoresis in 1.5% agarose gel (weight/volume), stained with Redsaf solution (Prolabo, France), and visualized under UV light (Gel Logic 200) [23].
2.6 Statistical analysis
The Fisher’s exact test with two-tailed p of Open Epi version 7.1.2.0 was used to determine the statistical significance of the results. A p value of <0.05 was considered statistically significant.
3. Results
3.1 Prevalence of bacterial isolates
From 315 children with diarrhea, 192 stool samples were positive to one suspected E. coli detection (60.9%). Four hundred and nineteen (419) strains of E. coli were isolated, from which 31 DEC (7.4%) were characterized. From these DEC, 21 DEC were ESBL-producing E. coli (67.7%).
3.2 Antimicrobial susceptibility
All the DEC strains tested for the 10 β-lactams antibiotics showed important resistances to the aminopenicillins. However, few cephalosporins and carbapenems were yet active on some pathotypes (Table 2).
β-Lactams subfamilies
Antibiotics
Prevalence of antibiotic susceptibility N (%)
DEC resistance prevalence N (%)
Resistant
Sensitive
EPEC (n = 8)
EHEC (n = 3)
EIEC (n = 4)
EAEC (n = 15)
ETEC (n = 1)
Penicillins
Amoxicillin
24 (77.4)
7 (22.6)
6 (76)
2 (66.6)
4 (100)
11 (73.3)
1 (100)
Amoxicillin-clavulanic acid
24 (77.4)
7 (22.6)
6 (76)
2 (66.6)
4 (100)
11 (73.3)
1 (100)
Piperacillin
20 (64.5)
11 (35.5)
5 (62.5)
2 (66.6)
3 (75)
9 (60)
1 (100)
Piperacillin-tazobactam
12 (38.7)
19 (61.3)
3 (37.5)
2 (66.6)
1 (25)
5 (33.3)
1 (100)
Cephalosporins
Ceftriaxone
13 (41.9)
18 (58.1)
2 (25)
1 (33.3)
2 (50)
7 (46.6)
1 (100)
Cefixime
13 (41.9)
18 (58.1)
2 (25)
1 (33.3)
2 (50)
7 (46.6)
1 (100)
Cefotaxim
14 (45.2)
17 (54.8)
2 (25)
2 (66.6)
2 (50)
7 (46.6)
1 (100)
Cefepim
14 (45.2)
17 (54.8)
2 (25)
2 (66.6)
2 (50)
7 (46.6)
1 (100)
Monobactam
Aztreonam
14 (45.2)
17 (54.8)
2 (25)
2 (66.6)
2 (50)
7 (46.6)
1 (100)
Carbapenems
Imipenem
5 (16.1)
26 (83.9)
1 (12.5)
1 (33.3)
0 (0)
3 (20)
0 (0)
Table 2.
Antimicrobials susceptibility of the studied isolates to β-lactams.
3.3 Correlation between resistance phenotype and resistance genetic supports
Nineteen (19) out of the 21 ESBLs-producing E. coli (90.5%) had ESBLs genes. The following resistance genes were characterized: 12 blaOXA (38.7%), 6 blaSHV (19.4%), 3 𝑏𝑙𝑎CTX-M (9.7%), and 2 blaTEM (6.5%). Our results showed that the genes responsible for the production of blaOXA β-lactamases 12/31 (38.7%) were more prevalent in comparison to the genes encoding blaTEM, blaSHV, and 𝑏𝑙𝑎CTX-M β-lactamases (Table 3). From the three classes of integrons (Int1, Int2, and Int3) assessed among the resistant strains carrying ESBL genes, only 18 Int1 (58.1%) and 2 Int3 (19.4%) were detected. The class 3 integron was detected in only EIEC. No class 2 integrons (Int2) were characterized from the resistant strains. The coexistence of the three resistance genes (blaSHV, blaOXA, and blaCTX-M) and Int1 was found in one EHEC (Table 3). The blaOXA gene (Figure 2) was associated with Int1 (Figure 3) in 11 cases (p = 0.001), while the blaSHV gene was associated with Int1 in 5 cases (p = 0.100).
DEC strains
Resistance phenotypes
Antibiotics
Genetic resistance supports
Resistance genes
Integrons
AMC
AMX
CTX
ATM
IPM
CRO
FEP
CFM
TZP
PIP
blaTEM
blaSHV
blaOXA
blaCTX-M
IntI1
IntI2
IntI3
Schiphra’s hospital (n = 9/18; 50%)
EHEC
ESBL, Carbapenemase
R
R
R
R
R
-
R
-
R
R
-
+
+
+
+
-
-
aEPEC
ESBL
R
R
-
-
-
-
-
-
-
-
+
-
-
-
+
-
-
tEPEC
ESBL
R
R
R
R
-
R
R
R
R
R
-
-
+
-
+
-
-
tEPEC
ESBL
R
R
-
-
-
-
-
-
R
R
-
+
-
-
+
-
-
EIEC
ESBL
R
R
-
-
-
-
-
-
-
R
-
+
-
-
+
-
+
EAEC
ESBL
R
R
-
-
-
-
-
-
-
R
-
+
-
-
+
-
-
EAEC
ESBL
R
R
-
-
-
-
-
-
-
-
-
+
-
-
+
-
-
EAEC
ESBL, Carbapenemase
R
R
R
R
R
R
R
R
R
R
-
-
-
+
+
-
-
EAEC
ESBL
R
R
R
R
-
R
R
R
R
R
-
-
+
-
+
-
-
Paul VI hospital (n = 10/13; 76.9%)
aEPEC
ESBL, PHN
R
R
-
-
-
-
-
-
-
R
-
-
+
-
+
-
-
aEPEC
ESBL, Carbapenemase
R
R
R
R
R
R
R
R
R
R
-
-
+
-
+
-
-
aEPEC
ESBL, PHN
R
R
-
-
-
-
-
-
-
R
-
-
+
-
+
-
-
EIEC
ESBL, PHN
R
R
R
R
-
R
R
R
R
R
-
+
+
-
-
-
+
EAEC
ESBL, PHN
R
R
-
-
-
-
-
-
-
R
-
-
+
-
+
-
-
ETEC
CASE, PHN
R
R
R
R
-
R
R
R
R
R
-
-
+
-
+
-
-
EAEC
ESBL, PHN
R
R
-
-
-
-
-
-
-
R
-
-
+
-
+
-
-
EAEC
ESBL, PBN
R
R
R
R
-
R
R
R
-
S
+
-
-
-
+
-
-
EAEC
ESBL, Carbapenemase
R
R
R
R
R
R
R
R
R
R
-
-
+
+
+
-
-
EAEC
ESBL
R
R
R
R
-
R
R
R
-
-
-
-
+
-
+
-
-
Table 3.
Correlation between E. coli pathotypes, antibiotics resistance, and genetic resistance supports.
blaOXA gene on agarose gel electrophoresis (1.5%). Lane M: molecular size marker (100 bp), 1: blaOXA1: positive control (813 pb), lanes: 2–8 are positive for blaOXA gene, lane T: negative control.
Figure 3.
Int1 gene on agarose gel electrophoresis (1.5%). Lane A: molecular size marker (100 bp), B: Int1: positive control (600 pb), lanes: C-O are positive for Int1 gene, lane T: negative control.
4. Discussion
The emergence and spread of multidrug-resistant (MDR) bacteria are major public health threats worldwide. Particularly, DEC that produce ESBL are of great concern, because their resistance to penicillins and narrow extended-spectrum cephalosporins reduces considerably the treatment options. The prevalence of ESBL in Enterobacteriaceae has been detected at local levels in various African countries; moreover, a study was conducted in 2014 on the prevalence of ESBL and what type of genes are involved in its occurrence [24]. The frequency of ESBL-producing E. coli was 67.7% in our study. Similar prevalence was reported in Egypt (69.6%) [25] and Palestine (66.7%) [26]. Nevertheless, our prevalence was higher than those in Burkina Faso (58%) [6], Iran (40.8%) [27], Saudi Arabia (30.6%) [28], Japan (20.4%) [29], Colombia (11.7%) [30], and Nepal (22.7%) [31]. Otherwise, our result is lower than the ESBL production in clinical isolates of E. coli reported somewhere else in Iran [32]. The prevalence of ESBL resistance in E. coli isolates in European countries is reported to be around 3.9% with variations between countries [33]. Overall, these percentages are lower than those found in middle-income countries like Thailand (71.25%) [34] and China (50.5%) [35]. This difference between ESBLs’ prevalence might be due to patient’s age, the type of samples, and the country health facilities in the management of diarrheal infections regarding antibiotics use. Indeed, in developing countries, most patients received antibiotics treatment without prescription [36, 37]; such common practices in nearly all developing countries cause a selective pressure on E. coli, whereas in more developed countries effective strategies for the control of antimicrobial are present, which effectively prevents the emergence of ESBLs [36].
It has been reported that bacteria such as E. coli and K. pneumoniae are major ESBL producers resulting in serious threat to the treatment regimen [38]. Indeed, ESBL enzymes are becoming increasingly expressed by many strains of pathogenic bacteria presenting diagnostic challenges to the clinical microbiology laboratories [39, 40]. Until recently, antimicrobial therapy has played an important role in the treatment of human bacterial infections. However, the drug resistance has emerged in the treatment of bacterial infections due to ESBL enzymes [39]. Indeed, these enzymes can degrade all β-lactam antibiotics leading to multidrug-resistant bacteria. Therefore, reporting of ESBL-producing isolates from clinical samples is critical for the clinicians. It constitutes the guidelines to select appropriate antibiotics for the treatment, including to take proper precaution to prevent the spread of these resistant organisms to other patients [31].
The present study shows 19 ESBLs genes (90.5%) out of the 21 ESBLs-producing E. coli. Analysis of the ESBL-encoding genes indicated that the majority of the ESBL-positive isolates harbored blaOXA (38.7%), followed by blaSHV (19.4%), blaCTX-M (9.7%), and 𝑏𝑙𝑎TEM (6.5%). The emergence of β-lactam resistance in Enterobacteriaceae is related primarily to the production of enzymes such as TEM and SHV variant, which were the most common ESBLs during the past decade. However, OXA and CTX-M β-lactamases have emerged as prevalent ESBL worldwide type compared with the TEM and SHV genotypes [41].
In the present study, OXA-type ESBL-producing DEC strains (38.7%) were the most frequently detected ESBL gene. This prevalence is lower than that reported in our previous study in rural area of Burkina Faso: 100% [9], also lower comparatively to 52% reported in Pakistan [42]. However, a recent study in young children reported 3% of commensal E. coli bearing the blaOXA gene in Bangladesh [41]. Thus, it appears that the emergence of ESBLs-producing bacteria among gut bacteria of young children can transfer resistance and related genes horizontally across pathogenic E. coli, and commensal E. coli leading to a public health concern. Most of the OXA-type ESBL-producing E. coli isolates (29%) in our study were detected from the Paul VI hospital (p = 0.002). This hospital is located in peripheral area of Ouagadougou, and most of the people living in the slums with poor sanitation conditions attend it for healthcare sought. Moreover, the provision of confessional care has less difficult accessibility for the peripheral neighborhoods and the population with low socioeconomic level. Otherwise, people in Burkina Faso do not consult a healthcare agent in the case of diseases such as gastrointestinal infections and use self-medication instead [37]. Our results showed 19.4% of SHV-type ESBL-producing E. coli which is a little similar to 21% detected in Pakistan [42]. By cons, this prevalence is higher than 0% [9] and 5.9% [17], previously reported in Burkina Faso but lower than 45% reported in Iran [27]. The blaCTX-M gene (Figure 4) has been detected in three E. coli isolates, while its prevalence was 25% in our earlier report [9] and 40.1% by a study conducted in Enterobacteriaceae from Burkinabe patients [17]. Moreover, few studies from other parts of world have shown different prevalence of blaCTX-M gene among isolates, including 98.8% (China), 84.7% (Chile), 13.6% (Tanzania), 76% (Pakistan), 97.8% (Chad), and 81.6% (Egypt) [25, 42, 43, 44, 45, 46]. Indeed, CTX-M β-lactamases are recognized as the most widespread extended-spectrum β-lactamases (ESBLs) among clinical isolates of Enterobacteriaceae [47]. Besides, an earlier report from Nigeria has shown the predominance of CTX-M15 in wild birds and cattle in Nigeria [48] suggesting that this gene could be transferred to humans by animals. Finally, our study revealed 6.5% of TEM-type ESBL-producing E. coli, while no blaTEM gene has been detected in our previous study [9]. However, this value is lower than 26.2 and 28% reported in Burkina Faso and Pakistan, respectively [17, 42]. The resistance to amoxicillin/amoxicillin-clavulanic acid observed in the two E. coli strains (6.5%) may be mainly mediated by the production of these plasmid-encoded TEM enzymes.
Figure 4.
blaCTX-M gene on agarose gel electrophoresis (1.5%). Lane M: molecular size marker (100 bp), A: blaCTX-M positive control (544 pb), lanes: B, D, and E are positive for blaCTX-M gene (544 pb), lane T: negative control.
Among the three class of integron, class 1 integron (58.1%) was majority characterized from the resistant strains in accordance with 56% reported in Bangladesh [41]. This result confirms those of previous studies showing that class 1 integron was predominantly represented in Enterobacteriaceae [49, 50]. However, a previous report in Burkina Faso has shown a lower prevalence (44.4%) of Int1 [51]. On the other hand, studies reported a high prevalence of Int1 (80%) in E. coli isolated from dairy products consumed in Burkina Faso [52] and in human, animal, and food in Spain [53]. This could increase the risk of emergence and spread of MDR E. coli, since humans are always in contact with these different ecosystems, especially when there is a lack of food hygiene and sanitation. Moreover, class 1 integrons can facilitate the spread of antibiotic-resistant genes meaning that it could have public health consequences [54].
The class 3 integron was detected in only EIEC. No class 2 integron (Int2) was characterized from the resistant strains. By cons, 22.2% of Int2 was detected in our previous study [51]. Moreover, a study also found the presence of Int2 gene in Senegalese Shigella spp. isolates [49].
Two strains of EIEC harbored both class 1 and 3 integrons. However, a previous study showed that E. coli harbored class 1 and 2 integrons simultaneously [50]. Otherwise, in the present study, one EIEC strain was resistant to aztreonam and imipenem and possesses ESBL-carbapenemase phenotype. This strain was resistant to all subfamilies (penicillins, cephalosporins, monobactam, and carbapenems) of β-lactams antibiotic tested and also showed simultaneous presence of blaSHV, blaOXA,blaCTX-M, and Int1. Indeed, strains that had this aztreonam-resistant phenotype possessed both the resistance gene [27]. Resistance to this antibiotic could be explained by genetic mutations [43]. It has been described that the coexistence of these two classes of integrons [42] and/or several genes suggests that they have integrated the same gene and give these strains a high level of resistance. However, blaTEM, blaSHV, blaOXA, blaCTX-M as well as integrons (Int1, Int2, and Int3) are involved in the antibiotic resistance of DEC, but the presence of resistant strains producing ESBL and lacking ESBL gene (blaTEM, blaSHV, blaOXA, and blaCTX-M) and integron suggests that there are other mechanisms for the dissemination of antibiotic resistance in DEC strains.
5. Conclusion
This study highlights the important involvement of genes and integrons into multidrug resistance strains of E. coli in two main hospitals of Ouagadougou. The most important finding was the detection of four E. coli multiresistant strains producing ESBL that were resistant to imipenem, aztreonam, and harbored class 1 integrons. Another important observation was the detection of two E. coli multiresistant strains producing ESBL but lacking a resistance gene and/or integrons. Our results have demonstrated the emergence and dissemination of multidrug-resistant E. coli strains hosting several genes responsible for the production of ESBL in clinical isolates. Ultimately, to fight effectively against the emergence of antimicrobial resistance, an integrated surveillance network should be set up, which would be of great benefit to national antimicrobial resistance control programs.
Acknowledgments
The authors gratefully thank “Réseau de Recherche sur les Maladies Entériques à potentiel épidémique en Afrique de l’Ouest (REMENTA)/Programme d’Appui à la Recherche en Réseau en Afrique (PARRAF)”for technical support. The authors also thank the parents and guardians of children as well as the authorities of the Paul VI and Schiphra’s hospitals for their honest cooperation.
\n',keywords:"diarrheagenic, E. coli, extended-spectrum β-lactamases, integron, Burkina Faso",chapterPDFUrl:"https://cdn.intechopen.com/pdfs/81030.pdf",chapterXML:"https://mts.intechopen.com/source/xml/81030.xml",downloadPdfUrl:"/chapter/pdf-download/81030",previewPdfUrl:"/chapter/pdf-preview/81030",totalDownloads:25,totalViews:0,totalCrossrefCites:0,dateSubmitted:"December 2nd 2021",dateReviewed:"February 10th 2022",datePrePublished:"March 30th 2022",datePublished:null,dateFinished:"March 30th 2022",readingETA:"0",abstract:"This study aimed to determine the resistance of diarrheagenic Escherichia coli (DEC) strains to β-lactams antibiotics and to perform the molecular characterization of extended-spectrum β-lactamases (ESBLs) and integrons genes. It was carried out from August 2013 to October 2015 and involved 31 DEC strains isolated from diarrheal stools samples collected from children less than 5 years. The identification and characterization of DEC strains were done through the standard biochemical tests that were confirmed using API 20E and polymerase chain reaction (PCR). The antibiogram was realized by the disk diffusion method, then an amplification of the β-lactamase resistance genes and integrons by PCR was done. Out of the 419 E. coli, 31 isolates (7.4%) harbored the DEC virulence genes. From these DEC, 21 (67.7%) were ESBL-producing E. coli. Susceptibility to ESBL-producing E. coli showed that the majority of isolates were highly resistant to amoxicillin (77.4%), amoxicillin-clavulanic acid (77.4%), and piperacillin (64.5%). The following antibiotic resistance genes and integron were identified: blaTEM (6.5%), blaSHV (19.4%), blaOXA (38.7%), blaCTX-M (9.7%), Int1 (58.1%), and Int3 (19.4%). No class 2 integron (Int2) was characterized. Because of the high prevalence of multidrug-resistant ESBL organisms found, there is a need of stringent pediatric infection control measures.",reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/81030",risUrl:"/chapter/ris/81030",signatures:"René Dembélé, Wendpoulomdé A.D. Kaboré, Issiaka Soulama, Oumar Traoré, Nafissatou Ouédraogo, Ali Konaté, Nathalie K. Guessennd, David Coulibaly N’Golo, Antoine Sanou, Samuel Serme, Soumanaba Zongo, Emmanuel Sampo, Alfred S. Traoré, Amy Gassama-Sow and Nicolas Barro",book:{id:"11272",type:"book",title:"Benign Anorectal Disorders - An Update",subtitle:null,fullTitle:"Benign Anorectal Disorders - An Update",slug:null,publishedDate:null,bookSignature:"Dr. Alberto Vannelli and Dr. Daniela Cornelia Lazar",coverURL:"https://cdn.intechopen.com/books/images_new/11272.jpg",licenceType:"CC BY 3.0",editedByType:null,isbn:"978-1-80355-706-9",printIsbn:"978-1-80355-705-2",pdfIsbn:"978-1-80355-707-6",isAvailableForWebshopOrdering:!0,editors:[{id:"34524",title:"Dr.",name:"Alberto",middleName:null,surname:"Vannelli",slug:"alberto-vannelli",fullName:"Alberto Vannelli"}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"}},authors:null,sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Methodology",level:"1"},{id:"sec_2_2",title:"2.1 Study design, area, and sample population",level:"2"},{id:"sec_3_2",title:"2.2 Sample collection and transport",level:"2"},{id:"sec_4_2",title:"2.3 Bacterial isolates",level:"2"},{id:"sec_5_2",title:"2.4 Antimicrobial susceptibility testing",level:"2"},{id:"sec_6_2",title:"2.5 Screening and confirmation of ESBL and integrons producers",level:"2"},{id:"sec_7_2",title:"2.6 Statistical analysis",level:"2"},{id:"sec_9",title:"3. Results",level:"1"},{id:"sec_9_2",title:"3.1 Prevalence of bacterial isolates",level:"2"},{id:"sec_10_2",title:"3.2 Antimicrobial susceptibility",level:"2"},{id:"sec_11_2",title:"3.3 Correlation between resistance phenotype and resistance genetic supports",level:"2"},{id:"sec_13",title:"4. Discussion",level:"1"},{id:"sec_14",title:"5. Conclusion",level:"1"},{id:"sec_15",title:"Acknowledgments",level:"1"}],chapterReferences:[{id:"B1",body:'Prestinaci F, Pezzotti P, Pantosti A. 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Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Sciences and Technologies, Joseph KI-ZERBO University, Burkina Faso
Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, Burkina Faso
'},{corresp:null,contributorFullName:"Wendpoulomdé A.D. Kaboré",address:null,affiliation:'
Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Sciences and Technologies, Joseph KI-ZERBO University, Burkina Faso
Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Sciences and Technologies, Joseph KI-ZERBO University, Burkina Faso
Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, Burkina Faso
Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Sciences and Technologies, Joseph KI-ZERBO University, Burkina Faso
Training and Research Unit in Applied Sciences and Technologies, University of Dedougou, Burkina Faso
Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Sciences and Technologies, Joseph KI-ZERBO University, Burkina Faso
Ali Konaté and Amy Gassama-Sow are deceased.
'},{corresp:null,contributorFullName:"Nathalie K. Guessennd",address:null,affiliation:'
Laboratory of Bacteriology-Virology, Unit of Antibiotics, Natural Substances and Surveillance of Resistance of Microorganisms to Antimicrobials, Pasteur Institute of Abidjan, Ivory Coast
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Laboratory of Molecular Biology, Epidemiology and Surveillance of Bacteria and Viruses Transmitted by Food, Centre for Research in Biological, Food and Nutritional Sciences, Graduate School of Sciences and Technologies, Joseph KI-ZERBO University, Burkina Faso
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Our journals are currently in their launching issue. They will be applied to all relevant indexes as soon as they are eligible. These include (but are not limited to): Web of Science, Scopus, PubMed, MEDLINE, Database of Open Access Journals (DOAJ), Google Scholar and Inspec.
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IntechOpen books are indexed by the following abstracting and indexing services:
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BKCI is a part of Web of Science Core Collection (WoSCC) and the world’s leading citation index with multidisciplinary content from the top tier international and regional journals, conference proceedings, and books. The Book Citation Index includes over 104,500 editorially selected books, with 10,000 new books added each year. Containing more than 53.2 million cited references, coverage dates back from 2005 to present. The Book Citation Index is multidisciplinary, covering disciplines across the sciences, social sciences, and arts & humanities.
Produced by the Web Of Science group, BIOSIS Previews research database provides researchers with the most current sources of life sciences information, including journals, conferences, patents, books, review articles, and more. Researchers can also access multidisciplinary coverage via specialized indexing such as MeSH disease terms, CAS registry numbers, Sequence Databank Numbers and Major Concepts.
Produced by the Web Of Science group, Zoological Record is the world’s oldest continuing database of animal biology. It is considered the world’s leading taxonomic reference, and with coverage back to 1864, has long acted as the world’s unofficial register of animal names. The broad scope of coverage ranges from biodiversity and the environment to taxonomy and veterinary sciences.
Provides a simple way to search broadly for scholarly literature. Includes peer-reviewed papers, theses, books, abstracts and articles, from academic publishers, professsional societies, preprint repositories, universities and other scholarly organizations. Google Scholar sorts articles by weighing the full text of each article, the author, the publication in which the article appears, and how often the article has been cited in other scholarly literature, so that the most relevant results are returned on the first page.
Microsoft Academic is a project exploring how to assist human conducting scientific research by leveraging machine’s cognitive power in memory, computation, sensing, attention, and endurance. Re-launched in 2016, the tool features an entirely new data structure and search engine using semantic search technologies. The Academic Knowledge API offers information retrieval from the underlying database using REST endpoints for advanced research purposes.
The national library of the United Kingdom includes 150 million manuscripts, maps, newspapers, magazines, prints and drawings, music scores, and patents. Online catalogues, information and exhibitions can be found on its website. The library operates the world's largest document delivery service, providing millions of items a year to national and international customers.
The digital NSK portal is the central gathering place for the digital collections of the National and University Library (NSK) in Croatia. It was established in 2016 to provide access to the Library’s digital and digitized material collections regardless of storage location. The digital NSK portal enables a unified search of digitized material from the NSK Special Collections - books, visual material, maps and music material. From the end of 2019, all thematic portals are available independently: Digital Books, Digitized Manuscripts, Digitized Visual Materials, Digital Music Materials and Digitized Cartographic Materials (established in 2017). Currently available only in Croatian.
The official DOI (digital object identifier) link registration agency for scholarly and professional publications. Crossref operates a cross-publisher citation linking system that allows a researcher to click on a reference citation on one publisher’s platform and link directly to the cited content on another publisher’s platform, subject to the target publisher’s access control practices. This citation-linking network covers millions of articles and other content items from