The ability of a new vaccine design based on control the intracellular physiological consequences of both the electrical properties and the electromagnetic radiation interactions between a virus and a host cell, which is a method to strengthen immune system develop protection against COVID-19 and new strains. The capacity of COVID-19 to bind to angiotensin-converting enzyme 2 (ACE2) and immune evasion mechanisms are only one of the properties required to stimulate a preventative immune response. In this chapter, a multidimensional new strategy is used to exemplify the empowerment function intracellular and extracellular level information can play in the support of immunogen against COVID-19 pathogens. Besides during this chapter, the nature of electromagnetic radiation is described as a vibrating string based on a string-theory and unification of electromagnetic radiation and gravitational waves by supporting with multiple cites strong evidence. Overall, we demonstrate a new approach to understand the important role of the physiological consequences of the interplay between the immune system and COVID-19 and designing vaccine strategy immunogens that take advantage of that information against COVID-19 and new strains.
- Electrostatic Discharge (ESD)
- Electromagnetic Radiation (EMR)
- Voltage-Viral Channels
- Zinc Homeostasis
Vaccine design is either a method, or style, or programming to activate the immune system to help the immune system develop protection against infectious disease. Vaccination includes various strategies of immunogen designs [1, 2, 3]. In this chapter, we describe a new strategy based on events for the COVID-19 ‘s journey to and interaction with the host cells, it seems to have the ability to induce a protective response against COVID-19 pathogens.
As known COVID-19 is adept at evasion host immune responses  and interfere with several receptor signaling pathways such as chemokine receptors to produce an active inflammatory response [5, 6]. Our previous work has shown the electrical properties of COVID-19 . On another hand, many studies have shown a stronger electrotactic attraction over chemical gradients for human T cells led to migration . This provides insight into the stronger electrotactic attraction for COVID-19 over chemical gradients for human T cell migration, and this reason virus interfered with several receptors signaling to prevent activation of the host innate immune system to allow for COVID-19 replication. This means the COVID-19 have electrical properties to evade interferon signaling and induction to avoid the antiviral agents of the host immune system. Evasion of the host antiviral immune response is critical for COVID-19 replication or spread. That is why some humans get very sick and may lead to death while some do not severely sick or even have no symptoms, and the reasons some humans have long-term impacts throughout their body, maybe get very sick and may lead to death.
In many types of the viral envelope, it has the viral ion channel in the small envelope membrane proteins like viroporins [9, 10, 11, 12]. From a role and function in the viroporin life cycle standpoint, it has viral protein with viral ion channel proteins have been shown to play a significant role to help in multiple processes of the journey for viral to and interaction with the host cells, such as CoV (SARS-CoV), influenza A, Human immunodeficiency virus (HIV)-1 [13, 14].
2. COVID-19’s journey to and interaction with the host cells
The electrical and electromagnetic radiation events are one of the first indications that virus is undergoing activation steps during infection to replicate the viral genome. There are four steps to and interaction with the host cells leads to having symptoms and long-term impacts throughout the body, maybe get very sick and may lead to death.
Moreover, the movement of ions generates an electric current by membrane depolarization (the influx of positive charge), in repeated motifs are regular by opening and closing of the channels because of a high potential difference. This electrical potential (high voltage) of the fluid surrounding a spike (S) protein is being electrically charged leads to generate corona discharge as results being a charged spike (S) protein have a sharp point, in adding to the protein–protein interactions (PPIs) leads to generate electrostatic force [24, 25, 26]. One of the side effects from an electrostatic discharge (ESD) is an induced to generate a corona discharge method, according to our previous work . The corona discharge is a series reaction are creating a free electron that ionizes atoms surrounding it to create a positive ion and a free electron . A corona discharge is classified into two general categories based on the type of attraction if all the electrons are attracted inward and the ions are repelled outwards, called a positive corona, else all the ions are attracted inward and all the electrons are repelled outwards, called a negative corona . When the electrons from an inner orbit are released, the vacancy will be filled by the electrons from an outer orbit led to the excess energy from this shift is emitted as electromagnetic radiation, thus serve to ionize other atoms . The emitted ionized radiation via the corona discharge method ionizes different cellular compartments inside the host cell. This emitted ionizing radiation can be damaged deoxyribonucleic acid (DNA) for host cell or many sorts of mutagens, thus cause host cell death via apoptosis .
Finally, novel viruses are produced from infected cells by transport, thus that can infect other cells. While both a protease and a zinc metalloenzyme inside the (ACE2) uses electrical charges or electrical signal to push a non-spontaneous redox reaction such as process electrolysis. As known protease is often used to catalyzes the breakdown of proteins, with supplementary helping mechanisms from viruses . Because the COVID-19 has an electric potential as discussed under Step III when COVID-19 migration to the (ACE2) this means occurs electrolysis.
3. Mechanisms of zinc metalloenzyme
In several studies have shown either the metal zinc, or ions zinc, or zinc transporters, or zinc signals, it has acted in different cellular functions and function of immune cells for both innate immune responses and adaptive immune responses [34, 35, 36, 37]. This chapter explains the mechanisms of zinc metalloenzyme being necessary for regulating zinc homeostasis in the body. The angiotensin-converting enzyme 2 (ACE2), contains a zinc metalloenzyme , in addition to the angiotensin-converting enzyme 2 (ACE2) protein contains protease and amino acid , which means that both a protease and a zinc metalloenzyme in the angiotensin-converting enzyme 2 (ACE2) are often used to generate an electric current from spontaneous redox reactions like as electrical battery, has two different metalloenzymes connected by a salt bridge (amino acid) externally completing an angiotensin-converting enzyme 2 (ACE2). An angiotensin-converting enzyme 2 (ACE2) is using this electric current seemingly as signals.
To understand an (ACE2) is extensions of spontaneous redox reactions, in this redox reaction, zinc metalloenzyme or metal is oxidized to ions (Zn2+) and ions other electrode is reduced to metalloenzyme or metal such as two electrodes (a cathode and an anode). When electrons are transferred directly from Zn to ions another electrode, is allowing the chemical energy interconversion to electrical energy. In the zinc metalloenzyme such as (half-cell), which is inside the (ACE2), the zinc dissolves via oxidation into the oxidation ions (Zn2+), other words releasing electrons. Results the zinc ion concentration increased to recompense via amino acid, which is inside the (ACE2), the zinc ions leave, and anions enter the zinc. In the other electrode such as (another half-cell), the ions onto this electrode reduce, taking up electrons that are leaving the zinc.
4. Vaccine strategy
To optimize vaccine design, it seems relevant to investigate the relationship between the electrophysiology properties for COVID-19, which can include the COVID-19 ‘s journey to and electrochemical interactions with the host cells, and the regulate zinc homeostasis on an intracellular level which includes (the mechanisms of zinc metalloenzyme inside the angiotensin-converting enzyme 2 (ACE2), zinc transporters, and zinc signals) through the physical processes. In several studies have shown the zinc is an essential supplement that serves to regulate different physiological mechanisms, it has antiviral properties [34, 35, 36, 37]. It is well known that zinc homeostasis or the zinc metalloenzyme homeostasis is crucial for a single cell or even inside cell level, protects the cell against oxidative damages, and supports on functions of the immune system .
Consequently, by investigating from this relationship, the findings that are the regulated zinc homeostasis on the intracellular level is targeted to inhibit or prevent COVID-19 before infection or even infection has already occurred. Zinc-regulation plays a critical and essential role in maintaining immune cell numbers and activities. The surprising efficiency of the influence on influenza virus, HCV, SARS-CoV-2, treatment of COVID-19, suggest a novel strategy for the vaccine design of COVID-19. Continuous monitor every period of the zinc homeostasis in the body, and correct zinc homeostasis if there is a defect in zinc homeostasis. This strategy can enhance antiviral immunity and innate immunity functions, it holds promise as an effective vaccine design strategy that should be further explored.
5. The nature of electromagnetic radiation (EM radiation)
To exploitation of biophysics, electrochemical, and physical processes for combat infectious diseases by blocking or disrupting their electrostatic discharge (ESD) properties link with electromagnetic radiation (EM) interactions something in a skillful manner. Here, electromagnetic radiation is imagined as a flow of tones produce via the vibrating strings with free ends, whereas tones produce via the vibrating strings with fixed ends in the darkness. In nature the electromagnetic radiation, more studies have suggested that the electromagnetic radiation has behaved either a wave or a particle, and some of the studies have suggested, it behaves as a wave and a particle at the same time. In this chapter, we will raise the conjecture (the purport of which will hereafter be called the “Principle of Diplopia and String”), to the status of a postulate, and introduce another postulate, which is only apparently irreconcilable with the former, namely, that the introduction of a “gravitational wave” will prove to be necessary since the view here to be developed will require a unification of electromagnetic radiation and gravitational waves. Those two postulates suffice for the attainment of a simple and consistent theory of electromagnetic radiation. Thus, those two principles contribute as key to solve the mystery of the wave-particle duality for objects, the darkness, the dark energy.
5.1 Vibrating strings with free ends
To describe the electromagnetic radiation based on the principle of diplopia and string. We must imagine the electron, which that is a closed vibrating string loop or an opened vibrating string. To render our presentation more precise and to distinguish this electron verbally from others which will be introduced hereafter, we call it the “electronString” and the free electronString. Thus, that is an electronString, which is the quotient of a combination between energy, and its mass (− 9.11 x 10–31 kg) .
If the electromagnetic radiation is imagined as a flow of tones, these tones we will call the “photonTone” with each photonTone carrying a separated packet of energy. To prove these tones means sound waves, and sound waves are part of the electromagnetic. First, according to several studies of electromagnetic radiation, which is a vibration of electric and magnetic fields at right angles. Accordingly, the tones or a sound wave are a disturbance that travels through a medium, this medium is a vibration of electric and magnetic fields, where here the tones, which is a vibration of electric and magnetic fields.
Let us apply the pair production [39, 40], based on the principle of diplopia and string when photonTone at extreme energy (gamma ray) can create two electronStrings. Here, the gamma ray or high photonTone, makes an electronString and its anti electronString, where the anti electronString is positively charged with the same mass as the electronString. By several studies have shown the sound waves carrying mass , and because the photonTone is a flow of tones carrying by a tones separated packet of energy, which means these photonTones carrying mass. Because we used a photonTone at extreme energy, this means the tones (sound wave) could carry much of mass to produces an electronString and its anti electronString. These two electronStrings can recombine with each other and create the photonTones. Thus, with the help of certain imaginary physical experiments we have settled what is to be understood by:
(es−): an electronString = −9.11 x 10–31 kg + pht (its energy),
es: a positronString = 9.11 x 10–31 kg + pht (its energy), and
pht: an emitted photonString.
Thus, when a number or amount (9.11 x 10−31 kg) is subtracted from itself, the results is zero.
When applying the photoelectric effect as shown in Figure 1 based on the principle of diplopia and string, when a photonTone incident on an atom transfers all its energy to this electronString, while the electronString containing the energy of the photonTone leaves the orbit of the atom.
Each electronString has a certain amount of binding energy, which is the minimum energy that is required to remove an electronString from an atom, this electronString is held in place by the electrostatic pull of the positively charged nucleus. For example, when an electronString absorbs a photonTone incident, this electronString becomes the move to a higher energy shell or become a free electronString, when this electronString drops back down to a lower-energy shell. Thus, this electronString will release energy represent a photonTone. Now, the electromagnetic radiation can be pictured as a vibrating photonTone by oscillating electric and magnetic fields that move in a straight line at a constant velocity.
5.2 Vibrating strings with fixed ends
If darkness is imagined as a flow of tones, these tones are producing via the vibrating strings with fixed ends by the gravitational wave, thus can generate standing waves. Where the gravitational waves squeeze and stretch strings in their path as they pass by to vibrate the string produce their tones, these tones represent energy. The tones are standing wave modes that arise from the combination of reflection and interference such that the reflected waves interfere constructively with the incident waves.
In the musical instrument, the vibrating strings are squeeze and stretch the string in their path as they pass by to vibrate the string produce their tones , as in the darkness is created from two waves with equal frequency, amplitude, and wavelength traveling in opposite directions by applying superposition. Where occur the destructive or constructive interference of counter-propagating waves one form of energy decreases and the other increases such that the total energy remains constant, we will call this a pair of the superposition of standing waves “diplopia waves”.
Effectiveness of vaccine against several types of viruses such as COVID-19 is mainly realized through investigating the relationship between the electrophysiology properties for a virus, and the regulate zinc homeostasis on intracellular level through the physical processes such as viral binding, membrane fusion, and infection. The regulated zinc homeostasis serves to stabilize the membrane potential which could play a role in preventing the virus of COVID-19 entry into the host cell. In additionally, the regulated zinc homeostasis serves to inhibit viral replication by protecting the deoxyribonucleic acid (DNA) from damage via an emitted ionizing radiation or mutagens as discussed under step III, protect the cells from exposure to oxidizing agents because the Zn2+ concentrations are directly belonging to the intracellular redox state. Interestingly, all this evidence used in this chapter supports the essential role for regulating zinc homeostasis to inhibit the physiological consequences as discussed under step III by this new vaccine strategy. This vaccine strategy is based on the properties of COVID-19, the voltage-viral charges channel activity required to inhibit the physiological consequences via dampening specific electrochemical functions associated with COVID-19’s journey to and interaction with the host cells and to strengthen immune system develop protection against COVID-19 and new strains.
Conflict of interest
The author declares no conflict of interest.
Irvine DJ, Aung A, Silva M: Controlling timing and location in vaccines. Advanced Drug Delivery Reviews. 2020;158: 91-115. DOI: 10.1016/j.addr.2020.06.019
Nabel GJ: Designing tomorrow's vaccines. New England Journal of Medicine. 2013;368:551-560. DOI: 10.1056/NEJMra1204186
Haynes BF, Burton DR: Developing an HIV vaccine. Science. 2017;355:1129-1130. DOI: 10.1126/science.aan0662
Taefehshokr N, Taefehshokr S, Hemmat N, Heit B: Covid-19: Perspectives on Innate Immune Evasion. Frontiers in immunology. 2020;11. DOI: 10.3389/fimmu.2020.580641
Ni W, Yang X, Yang D, Bao J, Li R, Xiao Y, Hou C, Wang H, Liu J, Yang D, Xu Y: Role of angiotensin-converting enzyme 2 (ACE2) in COVID-19. Critical Care. 2020 Dec;24(1):1-0. DOI: 10.1186/s13054-020-03120-0
Khalil BA, Elemam NM, Maghazachi AA: Chemokines and chemokine receptors during COVID-19 infection. Computational and Structural Biotechnology Journal. 2021;19: 976-988. DOI: 10.1016/j.csbj.2021.01.034
Al Sarkhi AK: Hypothesis: The electrical properties of coronavirus. Electromagnetic biology and medicine. 2020 Oct 1;39(4):433-436. DOI: 10.1080/15368378.2020.1830794
Wu J, Lin F: Recent developments in electrotaxis assays. Advances in wound care. 2014 Feb 1;3(2):149-155. DOI: 10.1089/wound.2013.0453
Delcour A. H, editors. book of Electrophysiology of Unconventional Channels and Pores. Springer: Cham; 2015. DOI: 10.1007/978-3-319-20149-8
Nieva JL, Madan V, Carrasco L: Viroporins: structure and biological functions. Nature Reviews Microbiology. 2012;10:563-574. DOI: 10.1038/nrmicro2820
Giorda KM, Hebert DN: Viroporins customize host cells for efficient viral propagation. DNA and cell biology. 2013;32:557-564. DOI: 10.1089/dna.2013.2159
Verdiá-Báguena C, Nieto-Torres JL, Alcaraz A, DeDiego ML, Torres J, Aguilella VM, Enjuanes L: Coronavirus E protein forms ion channels with functionally and structurally-involved membrane lipids. Virology. 2012 Oct 25;432(2):485-494. DOI: 10.1016/j.virol.2012.07.005
Sze C, Tan YJ: Viral membrane channels: role and function in the virus life cycle. Viruses. 2015;7(6):3261-3284. DOI: 10.3390/v7062771
Pinto LH, Holsinger LJ, Lamb RA: Influenza virus M2 protein has ion channel activity. Cell. 1992;69:517-528. DOI: 10.1016/0092-8674(92)90452-I.
Wang SJ, Saadi W, Lin F, Nguyen CM, Jeon NL: Differential effects of EGF gradient profiles on MDA-MB-231 breast cancer cell chemotaxis. Experimental cell research. 2004 Oct 15;300(1):180-189. DOI: 10.1016/j.yexcr.2004.06.030
Zigmond SH, Levitsky HI, Kreel BJ: Cell polarity: an examination of its behavioral expression and its consequences for polymorphonuclear leukocyte chemotaxis. The Journal of cell biology. 1981 Jun;89(3):585-592. DOI: 10.1083/jcb.89.3.585
Turner AJ: ACE2 cell biology, regulation, and physiological functions. The Protective Arm of the Renin Angiotensin System (RAS). 2015:185. DOI: 10.1016/B978-0-12-801364-9.00025-0
Hamming I, Timens W, Bulthuis ML, Lely AT, Navis GV, van Goor H: Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis. The Journal of Pathology: A Journal of the Pathological Society of Great Britain and Ireland. 2004 Jun;203(2):631-637. DOI: 10.1002/path.1570
Kuba K, Imai Y, Penninger JM: Angiotensin-converting enzyme 2 in lung diseases. Current opinion in pharmacology. 2006 Jun 1;6(3):271-276. DOI: 10.1016/j.coph.2006.03.001
Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts K, Walter P, Wilson J, Hunt T: Biologia molecular da célula. Artmed Editora; 2010; 1464. ISBN: 8582714238
AZZONE GF, PIETROBON D, ZORATTI M: Determination of the proton electrochemical gradient across biological membranes. Current topics in bioenergetics. 1984 Jan 1;13:1-77. DOI: 10.1016/B978-0-12-152513-2.50008-8
Mummert H, Gradmann D: Action potentials in Acetabularia: measurement and simulation of voltage-gated fluxes. The Journal of membrane biology. 1991 Dec;124(3):265-273. DOI: 10.1007/BF01994359
Höhner R, Aboukila A, Kunz HH, Venema K: Proton gradients and proton-dependent transport processes in the chloroplast. Frontiers in plant science. 2016 Feb 29;7:218. DOI: 10.3389/fpls.2016.00218
Kaiser KL: Electrostatic discharge. Technology & Engineering. 2005;344.
Loeb LB: Electrical coronas, their basic physical mechanisms. University of California Press; 1965.
Lüttgens G, Lüttgens S, Schubert W: Static Electricity: Understanding, Controlling, Applying. John Wiley & Sons; 2017 Aug 25.
Curtiss LA, Raghavachari K, Redfern PC, Rassolov V, Pople JA: Gaussian-3 (G3) theory for molecules containing first and second-row atoms. The Journal of chemical physics. 1998 Nov 8;109(18):7764-7776. DOI: 10.1016/S0009-2614(01)01345-8
Norbury CJ, Zhivotovsky B: DNA damage-induced apoptosis. Oncogene. 2004 Apr;23(16):2797-2808. DOI: 10.1038/sj.onc.1207532
Scheurich, P. and Zimmermann, U: Giant human erythrocytes by electric-field-induced cell-to-cell fusion. Naturwissenschaften. 1981 Jan 1;68(1), pp.45-47. DOI: 10.1007%2FBF01047168
Zimmermann U, Vienken J: Electric field-induced cell-to-cell fusion. The Journal of membrane biology. 1982 Dec;67(1):165-182.
Nieto-Torres JL, Verdiá-Báguena C, Castaño-Rodriguez C, Aguilella VM, Enjuanes L: Relevance of viroporin ion channel activity on viral replication and pathogenesis. Viruses. 2015 Jul;7(7):3552-3573. DOI: 10.3390/v7072786
Josephson RK, Schwab WE: Electrical properties of an excitable epithelium. The Journal of general physiology. 1979 Aug;74(2):213-236. DOI: 10.1085/jgp.74.2.213
Dalabehera NR, Meher S, Bhusana Palai B, Sharma NK: Instability of Amide Bond with Trifluoroacetic Acid (20%): Synthesis, Conformational Analysis, and Mechanistic Insights into Cleavable Amide Bond Comprising β-Troponylhydrazino Acid. ACS omega. 2020 Oct 1;5(40):26141-26152. DOI: 10.1021/acsomega.0c03729
Maywald M, Wessels I, Rink L: Zinc signals and immunity. International journal of molecular sciences. 2017;18(10):2222. DOI: 10.3390/ijms18102222
Overbeck S, Rink L, Haase H: Modulating the immune response by oral zinc supplementation: a single approach for multiple diseases. Archivum immunological et therapiae experimentalis. 2008 Feb;56(1):15-30. DOI: 10.1007/s00005-008-0003-8
Krężel A, Maret W: The biological inorganic chemistry of zinc ions. Archives of biochemistry and biophysics. 2016 Dec 1;611:3-19. DOI: 10.1016/j.abb.2016.04.010
Kumar A, Kubota Y, Chernov M, Kasuya H: Potential role of zinc supplementation in prophylaxis and treatment of COVID-19. Medical hypotheses. 2020 Nov 1;144:109848. DOI: 10.1016/j.mehy.2020.109848
Muhammad S, Omama M: A New Approach to Duality of Electron. Energy. 2020;14(15):16. DOI: 10.5923/j.ijtmp.20201006.04
Bomford K: Radiation protection. Walter and Miller’s textbook of radiotherapy. New York: Churchill Livingstone. 2003;69-88.
Bushberg JT: The AAPM/RSNA physics tutorial for residents. X-ray interactions. Radiographics. 1998;18 (2): 457-468. DOI: 10.1148/radiographics.18.2.9536489
Esposito A, Krichevsky R, Nicolis A: Gravitational mass carried by sound waves. Physical review letters. 2019 Mar 1;122(8):084501. DOI: 10.1103/PhysRevLett.122.084501
Perov P, Johnson W, Perova-Mello N. The physics of guitar string vibrations. American Journal of Physics. 2016 Jan;84(1):38-43. DOI: 10.1119/1.4935088