We are witnessing enormous efforts to identify prognostic and predictive biomarkers to inform treatment decisions in malignant mesothelioma. In this chapter, we will review and discuss the current literature and supportive evidence for the progress in development and use of biomarkers in malignant mesothelioma. There are currently several clinical trials evaluating treatment options in mesothelioma, and this will be an up-to-date review of these trials from published literature.
1. Epidemiology of mesothelioma
Malignant mesothelioma (MM) is an aggressive, rare cancer of pleural (80%), and peritoneal cells and less frequently in the pericardium and tunica vaginalis of the testis. MM has historically been linked to mineral fiber exposure. Asbestos is a collective term given to six mineral fibers including actinolite, amosite, anthophyllite, chrysotile, crocidolite, and tremolite . Exposure to other non-asbestos mineral fibers including erionite and fluoro-edenite has also been linked to MM . However, cases of MM have been found in patients who were not exposed to these mineral fibers. This led researchers to discover other epidemiologies of mesothelioma heavily linked to genetic mutations, including tumor suppressors like BRCA1-associated protein (BAP1) .
2. Biomarkers in mesothelioma
Recent research has been aimed at studying various biomarkers in malignant mesothelioma. Researchers hope that by identifying and studying specific biomarkers, new therapies can be developed that better target the unique pathways of malignant mesothelioma pathogenesis.
2.1 Vascular endothelial growth factor
The VEGF pathway is believed to play a critical role in angiogenesis and lymphangiogenesis in human malignant mesothelioma tumors . In one study, more than 95% of malignant pleural mesothelioma (MPM) samples stained positive for VEGF . An increase expression of VEGF was specifically observed in the epithelioid histology, more than biphasic and sarcomatoid. VEGF was not felt to have any prognostic significance in this study . In another study, VEGF was found to be an independent, poor prognostic factor in MPM . The phase III MAPS study showed that the addition of bevacizumab, a humanized anti-VEGF monoclonal IgG1 antibody, to frontline cisplatin/pemetrexed in unresectable malignant pleural mesothelioma improves overall survival (18.8 vs. 16.1; hazard ratio 0.77 [0.62–0.95]; p = 0.0167) compared to cisplatin/pemetrexed alone regardless of tumor histology . Analysis from the MAPS study showed that high VEGF concentrations were associated with worse progression free survival and overall survival but VEGF did not have a clinically meaningful predictive significance of response to bevacizumab . Other antiangiogenic agents like Sorafenib and axitinib have showed limited activity in malignant mesotheliomas [9, 10]. Ramucirumab is a recombinant human immunoglobulin G1 monoclonal antibody that binds to the extracellular domain of VEGFR-2 and prevents the binding of VEGFR ligands: VEGF-A, VEGF-C, and VEGF-D. A recently published Phase II abstract showed that the addition of Ramucirumab to gemcitabine significantly improved the overall survival in advanced MPM patients who progressed on first-line platinum-pemetrexed chemotherapy. This was observed regardless of patient age, tumor stage (locally advanced vs. metastatic), histotype (epithelioid vs. non-epithelioid), and time to progression at the first-line treatment .
2.2 Arginosuccinate synthetase
Certain cancer cells have a higher nutritional demand compared to normal cells. Arginine is an amino acid that plays an important role in biological and signaling pathways . Arginine is either synthesized in the body or consumed in the diet. Normal cells synthesize arginine through the urea cycle. Research suggests that certain cancer cells cannot internally make arginine because they lack the urea cycle enzyme argininosuccinate synthetase 1 (ASS1) which ultimately makes them dependent on exogenous supplies of arginine, an important amino acid for cancer survival and growth . ASS is a key enzyme that converts citrulline to arginine. This has led scientists to hypothesize that targeting the arginine synthesis pathway may be an effective therapeutic approach that targets cancer cells and spares normal cells.
Mesothelioma is one of the tumors that usually does not express ASS . Arginine degradation is dependent on different enzymes, including an enzyme called arginine deiminase (ADI) that degrades arginine to citrulline. In turn, citrulline can be recycled back to arginine in normal cells through ASS . A pegylated arginine deiminase (ADI-PEG 20) has been developed as an arginine depleting agent and is currently being tested in a randomized, double-blind, phase 2/3 study in subjects with malignant pleural mesothelioma with low argininosuccinate synthetase 1 expression to assess ADI-PEG 20 with pemetrexed and cisplatin (Clinicaltrials.gov ID NCT02709512).
2.3 Aurora kinase
Aurora kinase gene expression is upregulated in mesothelioma tumor tissue and is considered a negative prognostic factor [15, 16, 17]. The Aurora proteins are serine/threonine kinases that function in various stages of mitosis. Aurora kinase proteins A/B play an important role in mitosis, monopolar spindles formation, chromosomal segregation cytokinesis, and polyploidy. These proteins are overexpressed in mesothelioma . Aurora kinase inhibitors, like ZM447439, are able to inhibit cell growth in all mesothelioma cell lines . Alisertib (MLN8237) is a selective aurora kinase A inhibitor that is currently being evaluated in pretreated patients with unresectable MPM (Clinicaltrials.gov NCT02293005).
2.4 Wilms’ tumor protein
WT-1 is a zinc finger transcription factor protein that is responsible for controlling the expression of genes involved in cellular growth, differentiation, and/or apoptosis . WT1 is a nuclear protein that is processed and highly overexpressed on the cell surface of MPM. Immunohistochemical (IHC) staining for WT1 is routinely used in establishing the diagnosis of mesothelioma. WT-1 protein expression is detected by IHC in 78.1% of MPM and associated with improved overall survival and prognosis . Although WT1 protein is expressed on the cell surface in the context of MHC molecules, which makes it a target for T-cell based immunotherapeutic approach . A randomized phase II trial of adjuvant galinpepimut-S, WT-1 analogue peptide vaccine, after multimodality therapy for patients with WT-1 + MPM showed that a favorable safety profile with suggested improvement in progression-free survival and overall survival and a larger randomized trial is planned .
Mesothelin is a tumor differentiation protein that is normally expressed in low amounts on the pleural, peritoneal, and pericardial mesothelial cells. Mesothelin is highly expressed in malignant mesothelioma as well as other cancers like pancreatic, ovarian, and lung adenocarcinoma [23, 24, 25]. The differential expression of mesothelin between normal tissues and malignant cells made it an attractive candidate for cancer therapy. Mesothelin targeting agents including chimeric antigen receptor (CAR) T cells and vaccination strategies are currently in development for the treatment of MPM. CRS-207 is a live-attenuated strain of the bacterium
2.6 Programmed death-receptor ligand
PD-L1 is overexpressed in 40–50% of mesothelioma and associated with poor outcome. In one study, high PD-L1 expression was associated with non-epithelioid MM, poor clinical outcome, and increased immunological infiltrates . Several PD-L1 and PD1 targeting agents have been studied in mesothelioma with modest activity. Pembrolizumab, nivolumab, and ipilimumab are routinely used in the second-line therapy of malignant mesothelioma. PD-L1 testing is not required for prescribing pembrolizumab or nivolumab in the second-line therapy for patients with PMP . Limited data suggests that high PD-L1 expression (≥25% positive tumor cells) seems to be a predictor of higher overall response rate to nivolumab on nivolumab plus ipilimumab and even better objective response rate when the PD-L1 expression is >50% . Real-world data suggests that the high PD-L1 expression (≥50%) and non-epithelioid histology are associated with an improved objective response rate to pembrolizumab compared to intermediate (5–49%) and negative PD-L1 expression (<5%) in the second-line therapy of MPM .
2.7 BRCA1-associated protein
BRCA1-associated protein (BAP1) is a powerful deubiquitylating enzyme that acts to suppress the tumor growth. This means that it removes ubiquitin tags from specific proteins to modify and regulate their function or interaction with other molecules. BAP1 has been shown to have different tumor-suppressing functions when localized to the nucleus vs. cytoplasm. In the nucleus, it is promoted to double-stranded DNA break sites to aid in repair via homologous recombination, therefore inhibiting the growth of the damaged, mutated DNA [31, 32]. In the cytoplasm, BAP1 deubiquitylates type-3 inositol-1,4,5-trisphosphate-receptor (IP3R3) on the endoplasmic reticulum (ER). Once stabilized, IP3R3 allows the efflux of calcium (Ca2+) from the ER into the cytoplasm. This increase in Ca2+ promotes cytochrome c activation and induces cell apoptosis [32, 33]. More recently, it has been proposed that BAP1 also regulates ferroptosis, an iron-dependent programmed cell death via the repression of cystine transporter SLC7A11 .
Somatic inactivating mutations in
Germline mutations in
There are currently no standard therapeutic approach for
PARP inhibition is another potential targeted therapy option in patients with somatic or germline
A recent study published by Hassan et al
2.8 Neurofibromatosis type 2
Neurofibromatosis type 2 (NF2) is another tumor suppressor gene most commonly associated with the disorder Neurofibromatosis 2, in which malignancies including vestibular schwannomas and meningiomas are common. However, in more recent years, the somatic mutations of NF2 have been linked to malignant mesothelioma, in addition to multiple other organ systems [55, 56, 57].
Loss of merlin, a product of the neurofibromatosis 2 tumor suppressor gene is being evaluated as a biomarker for FAK inhibitor sensitivity in mesothelioma. When NF2 is absent or inactivated, these regulation pathways are disrupted which result in the constitutive activation of oncogenesis . Interestingly, when NF2 is reactivated and expressed in mesothelioma cells, invasiveness regresses . Targeting NF2 or downstream proteins like FAK has become an attractive therapeutic strategy in mesothelioma. Defactinib (VS-6063) is a FAK inhibitor. Merlin-low mesothelioma cell lines are more sensitive to defactinib than merlin-high cell lines in vitro and in vivo .
Defactinib (VS-6063) has been evaluated as a single agent in MPM. The phase II COMMAND trial was a randomized, placebo-controlled phase II study of defactinib in patients with unresectable mesothelioma who had had a stable disease or a PR following at least 4 cycles of platinum-based pemetrexed. Patients were randomized to receive maintenance defactinib or placebo. Patients were stratified by tumor merlin immunohistochemistry status (high vs. low) prior to randomization, and the study aimed to measure the effect of treatment allocation on the overall survival and progression-free survival. The study showed no difference in the progression-free survival or overall survival between the two treatment arms in the intent-to-treat population or in patients who had merlin-low tumors . Defactinib is currently being evaluated in combination with pembrolizumab in patients with pleural mesothelioma (Clinicaltrials.gov NCT04201145).
Another therapeutic approach that is currently being evaluated in NF2 mutant MM is NEDD8 activating enzyme (NAE) inhibition. Merlin is a negative regulator of mTORC1 and the loss of Merlin results in constitutive activation of the mTORC pathway [67, 68]. The exact mechanism by which Merlin suppresses mTOR signaling is unknown.
Merlin also suppresses tumorigenesis by accumulating in the nucleus and binding to the cullin E3 ubiquitin ligase CRL4(DCAF1) which suppresses its ubiquitination activity . Merlin loss drives tumorigenesis by activating the E3 ubiquitin ligase CRL4(DCAF1), thereby inhibiting the Hippo pathway component Lats . MLN4924, a NEDD8 activating enzyme (NAE) inhibitor that suppresses CRL4(DCAF1), attenuates the activation of YAP in NF2-mutant tumor cells . A phase I/II clinical trial is investigating MLN4924 (Pevonedistat) alone and in combination with chemotherapy in patients with mesothelioma. MLN4924 (Pevonedistat) is a NAE inhibitor that suppresses CRL4DCAF1 and attenuates the activation of YAP in NF2-mutant tumor cells.
2.9 Cyclin-dependent kinase inhibitor 2A (CDKN2A)
Cyclin-dependent kinase inhibitor 2A is a tumor suppressor gene that is commonly mutated in MM. It encodes both proteins INK4A and ARF . INK4A inhibits critical cell cycle regulators cyclin-dependent kinase 4 (CDK4) and cyclin-dependent kinase 6 (CDK6) . These two kinases function to activate retinoblastoma protein (RB) and allow for cell cycle progression . Without INK4A, cell cycle progression remains unchecked and allows for continuation and possible proliferation of damaged DNA. ARF acts by promoting MDM2 degradation; this degradation is necessary for the activation of p53, a widely studied tumor suppressor . With p53 activated, the cell cycle is arrested and growth is suppressed. Without ARF, p53 activation is limited and cell cycle progression can continue unchecked.
3. Multi-biomarker-driven clinical trials
The Mesothelioma Stratified Therapy Trial (MiST) is a large multi-drug phase II clinical trial evaluating the use of different biomarkers for the treatment selection in relapsed mesothelioma. BRCA1/BAP1-mutated mesothelioma treatment is being studied with Rucarapib, a PARP inhibitor. PARP enzymes are critical for cell function; they aid in DNA transcription, repair, and cell cycle regulation . It is believed that by inhibiting these critical enzymes, damage will accumulate within the cell and apoptosis will be induced. In patients with absent INK4A genes, apamaciclib is being studied. Apamaciclib is a selective CDK4/6 inhibitor, theoretically working to “replace” the function of INK4A in these mutated cells to stop the cell cycle progression and tumor growth . Patients with PDL1 positive mesothelioma are being treated with Atezolizumab and Bevacizumab. Atezolizumab is an anti-PDL1 antibody that selectively binds to PDL1 and prevents its interaction with B7.1 on the antigen-presenting cell (APC). This inhibits the cancer cell from utilizing PDL1 to evade the immune system [78, 79]. Lastly, for patients with no biomarkers, pembrolizumab and bemcentinib are being studied. Pembrolizumab is a monoclonal antibody against PD-1 and functions by binding PD-1 receptor on T-cells, inhibiting their binding with PDL1 [80, 81]. Bemcentinib is an AXL receptor tyrosine kinase inhibitor, a regulator of various critical cell functions including proliferation and motility, among others .
Over the last two decades, we have witnessed enormous efforts to identify prognostic and predictive biomarkers to inform treatment decisions in malignant mesothelioma. The medical and scientific community continue to search for optimal biomarkers to advance the field of precision medicine. Advances in molecular and diagnostic testing have not changed the current landscape of mesothelioma treatment. More biomarker-driven clinical trials are underway. The rarity of the disease makes it difficult to move these advances at a faster pace. Different pathways continue to be under investigation. These include: BAP1, NF2, CDKN2A, PD-L1, VEGF, WT-1, mesothelin, ASS, and aurora kinases. Biomarker-driven clinical trials, access to real-world data, and collaborative efforts should continue to move the field forward and help finding clinically actionable biomarkers.
There is no funding involved in this review. We would like to acknowledge the mesothelioma researchers and physicians who dedicated their career to treating mesothelioma and advance the discoveries in this field.
Conflict of interest
The authors declare no conflict of interest.