Open access peer-reviewed chapter

Health Risks of Potentially Toxic Metals Contaminated Water

Written By

Om Prakash Bansal

Submitted: October 1st, 2019 Reviewed: March 16th, 2020 Published: May 4th, 2020

DOI: 10.5772/intechopen.92141

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Groundwater which fulfills globally 50–80% need of drinking water, due to Anthropogenic and geologic activities, has been continuously contaminated by potentially toxic metals, causing a range of effects to animals and citizenry. In the developing countries, about 80% of diseases are waterborne diseases. Bio accumulation of these metals in citizenry due to intake of contaminated vegetables, fruits, fishes, seafood and drinking water and beverages causes a serious threat to citizenry. Toxicity of these metals is due to metabolic interference and mutagenesis, interference in the normal functioning of structural proteins, enzymes, and nucleic acids by binding them, adversely affecting the immune and hematopoietic systems in citizenry and animals. The toxic metals also enrich antibiotic resistant microbes particularly bacteria by Co-selection (occurring by Co-resistance and cross-resistance) as it promotes antibiotic resistance in bacteria even in absence of antibiotics. These metals in living cells cause cytotoxicity, oxidative stress resulting in the damages of antioxidants, enzyme inhibition, loss of DNA repair mechanism, protein dysfunction and damage to lipid per oxidase. Endocrine disruption, neuro-developmental toxicity, biosynthesis of hemoglobin, metabolism of vitamin D, renal toxicity, damage to central nervous system, hearing speech and visual disorders, hypertension, anemia, dementia, hematemesis, bladder, lung, nose, larynx, prostate cancer, and bone diseases are some other health’s risks to human.


  • pollution
  • human
  • potentially toxic metals
  • health risks
  • heavy metals resistance
  • fishes

1. Introduction

Water the “life-blood of the biosphere” may be an alcahest which dissolves different chemicals and environmental pollutants. Globally, groundwater is the main source of domestic drinking water both in rural and urban areas and fulfills approximately 80% need of drinking water in the rural areas and 50% of urban water need. As surface water infiltrates to unconfined aquifers easily, these aquifers are contaminated very easily. The pollution of groundwater causes significant alteration in the environment. The most sources of lakes, rivers, ponds, and streams are the groundwater. When contaminated groundwater is supplied to those sources, the surface water is additionally contaminated which causes harm to birds, animals, and plants. Because of population growth over the last 50 years, the abstraction of groundwater has increased leading to reduce natural discharge flows and groundwater quality. Groundwater quality is additionally suffering from recharge rate and recharge quality. Existence of human on earth without potentially toxic metals is not possible. These potentially toxic metals (Cd, Cu, Pb, Zn, Cr (III), Cr (VI), and Hg) have high relative atomic mass and density. Hindu Business on Feb 20, 2019 reported that “more than forty million people in rural India drinks to water contaminated by heavy metals, arsenic, fluoride, etc.” The results are devastating. Diarrhea, often caused by exposure to fecal matter, kills 600,000 Indians per annum and waterborne diseases throughout the Ganges basin. In India, 25% of water sources is River Ganga. These metals participate within the redox reactions and are an important part of enzymes. Cobalt is a constituent of vitamin B12, and manganese acts as an activator of the enzymes within the physical body [1]. Copper is important for enzyme ascorbate oxidase, cytochrome oxidase, plastocyanin oxidase, and photosynthesis in plants. As these metals cannot be degraded, they persist in the environment (in soils, industrial effluents, groundwater) for a long period; easily bio accumulated and bio magnify within the food chains poses a significant threat to the consumer and pollution of water sources by potentially toxic metals became a worldwide problem [2]. The toxic effect of these metals could also be because of metabolic interference and mutagenesis. These metals interfere in the normal functioning of structural proteins, enzymes, and nucleic acids by binding them. Even a smaller amount of potentially toxic metals/metalloid arsenic, lead, cadmium, nickel, mercury, chromium, cobalt, and zinc beyond their permissible limit in body became harmful. These metals also enrich antibiotic resistant microbes even in the absence of antibiotics. This study discusses the health risks of potentially toxic metals contaminated water to the citizenry accumulated via intake of contaminated vegetables, fruits, fishes (freshwater or marine), drinking water, and beverages. The consequences of those metals on antibiotic resistant genes and bacteria have also been discussed.


2. Potentially toxic metals

Potentially toxic metals are essential in a small amount for various biochemical and physiological functions within the plants, animals, and humans. These metals participate in the redox reactions and are an important part of enzymes. A number of researchers [3, 4, 5, 6] have reported that natural contaminates of natural water are potentially toxic metals and organometallic compounds. Based on their health importance, the potentially toxic elements are classified into four groups, (i) essential: Cu, Zn, Co, Cr, Mn, and Fe. These metals beyond their permissible limit become toxic, (ii) non-essential: Ba, Al, Li, (iii) less toxic: Sn, and (iv) highly toxic: Hg, Cd, Pb, As (metalloid).

2.1 Routes of uptake

Routes of uptake of those toxic metals by human and animals are:

  1. Ingestion: it occurs via gastrointestinal route, that is, through the mouth by eating contaminated food, vegetables, fruits, seafood including fish, and by drinking contaminated water and beverages.

  2. Dermal: dermal uptake means absorption through skin/gills, the aquatic animals’ bio accumulates these toxic metals via dermal contact.

  3. Inhalation: inhalation uptake occurs via inhalation of the polluted air as dust fumes and through exposure at work place. In the fish, these metals enter the body directly from water or sediments via the gills/skin or via its alimentary tract from the fish food/prey.


3. Sources of potentially toxic element contaminants within the water

Contamination of the environment by potentially toxic metals has increased after war II because of rapid industrialization and urbanization and increased rate of mobilization and transport [7]. The main sources of groundwater of contamination by heavy metals are:

3.1 Natural

Rock weathering, forest fires, volcanic eruptions, biogenic sources, and wind born soil particles are the natural sources of potentially toxic metals within the environment. Within the rocks, these metals are present as hydroxides, sulphides, oxides, silicates, phosphates, and chelated with organic compounds.

3.2 Anthropogenic activities

Industrial manufacturing of products to satisfy with the stress of the massive population like to cement production, iron industry, steam power plants, glass production, paint, and tanning industries is one among the causes of environmental pollution because of human activities. Agricultural activities (use of sewage sludge as manure), irrigation by sewage wastewater, mining, and metallurgical processes, garbage and waste mud incineration facilities, combustion of fuels, surface emission, and traffic and runoffs are other ways to release the pollutants within the different environmental compartments. The main route of the groundwater and aquatic contamination by potentially toxic metals are the leaching from toxic industrial waste dumps and municipal landfills and leaching of agricultural chemicals from soils into the upper aquifers [8].

As the concentration of potentially toxic metals within the environment is continuously increasing, and therefore, the soil retention capacity of those metals is decreasing and the resultant is the leaching of those metals within the groundwater [9]. Fertilizers and pesticides applied within the fields contain these potentially toxic metals (Cr, Cd, Cu, Zn, Ni, Mn, Pb, and As) as impurities [7]. Another source of groundwater contamination by potentially toxic metals is the urban runoffs which contain Pb, Cu, Zn, Fe, Cd, Cr, and Ni. The intrusion of seawater in aquifers also causes a rise in concentration levels of potentially toxic metals in the groundwater. Another anthropogenic source of the heavy metals in the environment is the burning of wastes at residential levels and dumpsites.


4. Potentially toxic metals within the ground, surface water, and sediments

The pollution of water resources by potentially toxic metals affects plants, animals, and human health adversely [10]. These metals even at a low concentration are toxic to aquatic organisms as these metals alter the histopathology of the tissues of the organisms [11, 12]. The one among the main sources of potentially toxic metals within the aquatic environment are the sediments which act as a sink and reservoir of those metals [13].

4.1 Potentially toxic metals within the drinking, ground, and surface water

A review of the literature showed that globally number of groundwater, drinking water, and surface water samples contains the potentially toxic metals beyond their permissible limit which affects adversely the human and ecological health. The arsenic concentration in the groundwater samples ranged from 0.0005 to 1.15 mg/L [14, 15, 16]. Author himself [17, 18] studied the concentration of potentially toxic metals in the groundwater samples for 20 years (1986–2005) and found that (i) concentration of those metals are increasing with time, (ii) concentration of those metals decreased with depth, and (iii) the concentration became beyond the permissible limit after the year 2000. The concentration of the toxic metals in the groundwater, drinking water, and surface water samples are recorded in Table 1.

SampleSourceConcentration of metal (mg/L) or (mg/kg)Reference
Drinking waterLagos state, Nigeria0.00.009–0.0210.027–0.0600.11–0.410.098–0.140.72–1.02[19]
Drinking water and surface waterYobe State, Nigeria0.0074–0.990.001–0.120.012–0.0870.0240.0[20]
Surface waterNile River and its canals0.001–0.0480.054–0.329[21]
Drinking waterEgypt0.002–0.0490.09–0.41
Drinking waterNigeria0.02–0.124;0.009–0.0570.08–20.10.04–0.570.091–0.485[22]
Surface waterPearl River, China0.0005–0.00750.0035–0.0110.003–0.0050.0165–0.06070.0006–0.00110.0014–0.0045[4]
Surface waterDares salaam, Tanzania0.320.590.031.140.46–0.550.99–1.26 (Fe)[23]
Surface waterGanga River, India0.0–18.550.003–6.282.25–63.564.700.166–107.340.06–5.94.73[5, 6, 24]
Ground waterChembarambakkam lake (India)0.00–1.320.00–0.190.018–0.0880.008–2.45,0.172–0.4860.00–0.03[25]
Surface water0.00––0.030.01–0.700.16–0.220.00
Surface waterTamilnadu0.229–1.4840.001–0.1280.001–0.650.031–0.781;0.01–0.695[26]
Surface waterSolan district (India)0.00–0.000040.0021–0.00720.0 0–0.0041,0.00–0.0014[27]
Ground water0.00001–0.000330.0006–0.00150.0001–0.00290.0–0.0009
Surface waterBodo Creek water0.03–0.061.03–1.63[28]
Surface waterKenya0.31–0.531.37–1.920.57–2.43[29]
Ground waterMaru Town, Nigeria0.0–0.990.0–0.330.012–0.0870.00–0.0056[30]
Ground waterSinghbhum, India0.01–0.080.04–0.280.08–0.420.03–0.140.07–4.45 (Fe)[33]
SedimentsRiver Raohe, Chia0.00–1.6013.5–97.115.6–793.511.2–52.916.0–222.218.4–66.412.9–318.0[13]
SedimentsMashavera Basin Georgia1.5–1.726.4–28347.8–410.7423.3–458.929.6–37.222.3–22.50.00–0.02 (Hg)[34]
SedimentsRiver of Philippines32.8–131.829.4–217.176.8–263.312.1–98.14.1–25.3 (Co)[36]
SedimentsRiver Gomati1.9–8.49.0–95.435.8–90.93.7–15.0[37]
SedimentsRiver Ghaghara0.21–0.2861.3–84.72.8–11.713.3–17.610.7–14.315.3–25.611.4–18.4 (Co)[38]
SedimentsRiver Ganga1.769.929.867.826.726.7[39]
SedimentsBay of Bengal0.03–0.060.61–0.790.38–0.660.01–1.420.01–0.23[40]
VegetablesBangladesh0.03–2.40.03–22.60.4–52.30.33––61.50.03–1.020.63–1.330.02–32 (Mn)[41, 42, 43]
VegetablesSupermarket of the Florida0.002–0.0400.012–0.2230.13–2.471.31–3.950.0019–0.0650.012–0.2910.002–0.0200.0005–0.033 (Co)[44]
Vegetables0.0006–0.0280.009–0.1260.22–2.651.12–3.880.0005–0.070.010–0.0960.0012–0.0160.0012–0.0065 (Co)
VegetablesIran0.030.550.85–4.10.5–1.50.69 (Co)[45, 46]
VegetablesPakistan0.045–0.392.72–6.6222.2–65.219.5–411.8–5.018.7–137.3 (Mn)[47]
VegetablesNigeria0.11–0.435.1–9.94.7–7538.1–335.22.6–9.219.3–33.3 (Mn)[48]
VegetablesLocal vegetables, Iraq1.5–6.01.02–1.137.3–37.225.3–43.80.5–1.486.3–7.94.732.83–3.09 (Co)[49]
VegetablesImported vegetables, Iraq1.6–5.81.03–1.1517.3–33.517.3–33.50.32–1.557.1–10.33.08–3.10 (Co)

Table 1.

The concentration of different potentially toxic metals (mg/L) in drinking, ground, and surface water and in sediments and vegetables (mg/kg).

4.2 Potentially toxic metals within the sediments

The accumulated amounts of the potentially toxic metals in the sediments are reported in Table 1.


5. Bioaccumulation of probably toxic metals in vegetables and fruits irrigated by contaminated ground and surface water

A long-term study made by the author himself [17, 18] found that the concentration of potentially toxic metals in the soils of agricultural fields of Aligarh irrigated by sewage effluent is continuously increasing, and therefore, the concentration of those metals in edible parts of the crops grown such soils were beyond toxic limits, and maximum accumulation was within the potato followed by maize [53]. Bansal [53] during their studies on the concentrations of Pb, Cd, Ni, Cr, Zn, and Cu in the vegetables palak, cabbage, brinjal, lady’s finger, tomato, bitter gourd, radish, and cauliflower grown in the soils of periurban areas of Aligarh irrigated by sewage effluent water found that the concentration of the metals Cd, Pb, and Ni in all the studied vegetables were beyond their permissible limits for human consumption. The Target Hazard Quotient (THQ) values also denote that consumption of those vegetables will cause a potential risk for human health risk. Kabir and Bhuyan [54] found that the concentration of Cu in the hen’s egg yolk (1.85–3.65 mg/kg) and albumin (0.5–1.15 mg/kg) were beyond permissible limits. The concentrations of these metals in the vegetables grown globally are given in Table 1.


6. Bioaccumulation of potentially toxic metals in freshwater fish, marine fish aqueous flora and fauna

Bioaccumulation of the potentially toxic heavy metals within the ecosystem of the Riverine has a negative impact on the ecological health of aquatic animals and causes decrease in their populations [55, 56]. Several researchers have reported fish deformities, decline of fish populations, and reduce in their growth rates if the concentration of potentially toxic metals increased beyond their tolerable limit [57, 58]. The concentrations of those metals in freshwater fish are reported in Table 2. The info in Table 3 denotes the concentration of those metals in marine fish and other organisms.

Fish speciesSourceTissueConcentration of metal (mg/kg)Reference
Cyprinus carpioSardaryab, tributary of River KabulGills0.1540.0240.0740.041[59]
Labeo rohitaSardaryab, tributary of River KabulGills0.1330.0180.0580.024
Cyprinus carpioIndus River Mianwali, PakistanGills2.9–9.40.77–1.40.5–1.87[60]
Wallago attuIndus River Mianwali, PakistanGills4.5–9.53–5.90.45–1.92
Labeo rohitaKolleru Lake, IndiaGills0.380.[61]
Channa striatusKolleru Lake, IndiaGills0.320.590.031.141.300.12
Oreochromis niloticusBurullus Lake, EgyptMuscles0.450.850.394.700.46[62]
FinfishLower Gangetic Delta, IndiaWhole Body0–1.321.3–53.12.0–111.50–3.05[63]
ShrimpLower Gangetic Delta, IndiaWhole Body0–1.56.2–109.211.7–213.70–10
OysterLower Gangetic Delta, IndiaWhole BodyBDL8.7–69.121.4–202.80–8
Tilapia zilliiNiger River, NigeriaGill0.0948.92NDND[64]
Malapterurus electricusNiger River, NigeriaGill0.0565.77NDND
Clarias gariepinusNiger River, NigeriaGill0.0555.55NDND
Clarias batrachusLocal fish ponds of Ludhiana city and Sutlej RiverLiver3.743.693.4867.7811.123.13[58]
Barbuss harpeyiTigris River in BaghdadGills2.3–2.42.2–2.51.1–1.21.05–1.11.5–1.6[65]
Barbus xanthopterusBodo Creek, Niger Delta, NigeriaGills2.2–2.52.1–2.51.2–1.31.1–1.21.3–1.6
Callinectes amnicolaBodo Creek, Niger Delta, NigeriaLeg0.43–3.780.3–1.13[27]
Chrysichthys nigrodigitatusDensu River, GhanaMuscles0.592.340.190.37[11]
Alosa immaculataDanube RiverWhole Body0.095.340.65[66]
Cyprinus carpioDanube RiverWhole Body0.0845.100.58
Cyprinus carpioDanube River, Belgrade, SiberiaWhole Body0.0140.2070.036[67]
Silurus glanisDanube River, Belgrade, SiberiaWhole Body0.080.2350.014
Silurus glanisDanube RiverWhole Body0.[68]
Sander luciopercaDanube RiverWhole Body0.
Tilapia mossambicaWater bodies of Aurangabad, IndiaGill3.66–4.580.32–0.46[69]
Oreochromis niloticusLakes of Coimbatore, IndiaMuscle1.26–1.598.49–9.6918.38–25.945.91–9.69[70]
Lutjanus griseusPearl Delta river ChinaWhole Body0.030.030.39[71]
Lutjanus stellatusPearl Delta river ChinaWhole Body0.070.041.53
Thunnus albacaresEgyptWhole Body0.060.32[72]
Oncorhynchus mykissHamadan Province, IranWhole Body0.17–13.740.34–70.17[73]

Table 2.

The concentration of different potentially toxic metals in different parts of freshwater fish.

Fish speciesSourceTissueConcentration of metal (mg/kg)Reference
MulletSoutheast Coast of Indian OceanGills0.0130.0920.0870.043[74]
Whole Body0.0050.0850.1760.026
CrabSoutheast Coast of Indian OceanMuscle0.0270.2430.2280.237
Whole Body0.0040.0610.2590.013
ShrimpSoutheast Coast of Indian OceanSkin0.0010.0820.2330.268
Whole Body0.0010.20.0880.007
Euthynnus affinisTok Bali Port, MalaysiaWhole Body0.0070.7062.40.3[57]
Pampus argenteusTok Bali Port, MalaysiaWhole Body0.0040.0384.830.024
Decapterus macrosomaTok Bali Port, MalaysiaWhole Body0.0030.0645.290.001
Leiognathus dauraTok Bali Port, MalaysiaWhole Body0.0040.0185.450.003
Fenneropenaeus indicusTok Bali Port, MalaysiaWhole Body0.040.3414.40.008
Lates calcariferWhole Body0.0070.496.50.20[75]
Johnius belangeriiRed Sea, Jeddah Coast, Saudi ArabiaWhole Body1.3523.331.45[76]
Chirocentrus dorabWhole Body1.4522.911.0
Arius maculatusWhole Body1.1525.950.85
Parastromateus nigerWhole Body2.5522.291.0
OysterGulf of ChabaharSoft tissue0.08–0.4512.7–38.059.2–133.587–1912.36–17.5[77]
Liocarcinus depuratorSamsun coasts of the Black Sea TurkeySoft tissue0.077.7190.48[78]
Rapana venosaSoft tissue0.0854.390.12
Mytilus galloprovincialisSoft tissue0.089.2140.50
Otolithes ruberPersian Gulf, IranSoft tissue0.21–0.471.98–2.98[79]
Lutjanus johniiPersian Gulf, IranSoft tissue0.17–0.382.53–3.12
Lagocephalus sceleratusNorth-eastern Mediterranean part of TurkeyMuscle0.045–0.1390.20–0.360.276–0.51851.4–86.631.46–2.56[80]
Hirundichthys coromandelensisSoutheast coast of IndiaSoft tissue0.020.26–0.283–3.280.20–0.24[81]
Cypselurus spilopterusSoutheast coast of IndiaSoft tissue0.020.26–0.332.15–3.30017–0.19
SardinaAlgerian coasts, AlgeriaSoft tissue0.552.130.62[82]
Xiphias gladiusAlgerian coast, AlgeriaSoft tissue0.573.900.56
Brachydeuterus auritusFishing Habour Ghana,Muscle0.422.280.20.31[11]
Pennahia aneaCoastal Waters, MalaysiaMuscle0.03–0.210.94–4.3817.7–26.30.14–0.41[83]
Arius maculatusMuscle0.04–0.090.83–3.6823–48.60.15–0.36
Decapterus maraudsiTerengganu Coastal Area, MalaysiaSoft tissue0.20.647.970.17[84]
Megalaspis cordylaTerengganu Coastal Area, MalaysiaSoft tissue0.311.1610.420.02
BramidaeTerengganu Coastal Area, MalaysiaSoft tissue1.530.9815.140.09
Selaroides leptolepisTerengganu Coastal Area, MalaysiaSoft tissue0.660.6811.280.14
Epinephelus lanceolatusTerengganu Coastal Area, MalaysiaSoft tissue0.640.8312.510.11
RastrelligeTerengganu Coastal Area, MalaysiaSoft tissue0.250.59.390.73
Nibea soldadoTerengganu Coastal Area, MalaysiaSoft tissue0.120.295.91ND
Pristipomoides filamentosusTerengganu Coastal Area, MalaysiaSoft tissue0.080.254.88ND
Priacanthus tayenusTerengganu Coastal Area, MalaysiaSoft tissue0.080.426.630.13
Siganus canaliculatusTerengganu Coastal Area, MalaysiaSoft tissue0.100.6811.600.15
Thunnus obesusWestern and Central pacific oceanSoft tissue0.929
Trichiurus lepturusCoastal Waters of Ondo State, NigeriaMuscle0.[85]
Pentanmius guigariusMuscle0.00.00.580.350.1
Pseudotolithus senegalensisMuscle0.00.00.460.360.10
Pseudotolithus typusMuscle0.310.360.00.360.07
Cyprinus carpio L,Ala gul wetland (Iran)Muscle0.0–0.1401.23–39.41.15–47.70–21.86[86]
Cyprinus carpio L,Alma gul wetland (Iran)0.0–0.021.23–4.419.15–117.42.1–8.7

Table 3.

The concentration of different potentially toxic metals in different tissues of marine organisms.


7. Potentially toxic metals-resistance

The potentially toxic metals (Cd, Cu, Pb, Zn, Cr (III), Cr (VI), and Hg) aren’t only toxic to human health but also enrich antibiotic resistant microbes particularly bacteria. Co-selection of an antibiotic and metal resistance in bacteria is extremely important because it promotes antibiotic resistance in bacteria even in the absence of antibiotics. Co-selection occurs by two mechanisms:

  1. Co-resistance: when antibiotics and these metals co-exist within the same environment, these metals influence some antibiotic resistant bacteria to survive in more polluted environment. Co-resistance occurs when two or more different resistant genes are present on the same genetic elements (plasmid, transposon, Integron) or are present within the same bacterial strain which provides resistant to different compounds. The rise of antibiotic resistant genes is directly correlated with the concentration of those metals [87].

  2. Cross-resistance: cross resistance occurs when antibiotics and potentially toxic metals target the same microbes leading to generic detoxification of genes by reducing intracellular concentration of antibiotics and metals, and enhanced efflux occurs during cross-resistance [87].

Besides these two mechanisms, co-selection is additionally promoted by co-regulatory mechanism which occurs when different resistant genes is controlled by one regulator gene [88]. The impact of the potentially toxic metals on the antibiotic resistant bacterial strain is a given in Table 4.

Toxic metalMechanism of actionMechanism of resistanceAntibiotic categories /generic namesPathogen(s)
CuProduces hyperoxide radicals by interaction with cell membrane. Enzymatic activities are inhibited and cellular functions are disrupted.Resistant genes are located on plasmids and transposons and transfers in between bacterial species.TetracyclineKlebsiella spp. [89, 90]; Fecal Enterococci [91]; E. coli [92]; E. faecium [93]
CarbapenemPseudomonas aeruginosa [94]
Vancomycin, AmphenicolEnterococcus faecium [93, 95]
ChloramphenicolBacillus spp. isolated from ship [96]
Erythromycinsoil bacteria of Scotland [90]; Enterococcus faecium [95]
FluoroquinoloneE. coli [92]; E. faecium [93]
Quinolone; SulphonamideE. coli [92]
CephalosporinFecal Enterococci [91]
Penicillin; Cephalosporin; NitrofuranEnterobacter spp.; P. aeruginosa [97]
MacrolideE. faecium [98]
HgHg inactivates the enzymatic activities, interferes in the protein synthesis and DNA function, and disrupts cell membrane. Destroys the biological membranes as the mercuric ions are lipid soluble so easily passed through biological membranes.Hg resistant genes are located on plasmids and transposons. The resistance mechanism involves the reduction of Hg2+ ions to Hg in the cytoplasm of the bacteria by the enzyme mercuric reductase which is encoded with the merA gene.Sulphonamide, Chloramphenicol, Ampicillin, Streptomycetin, AugmentinSalmonella enterica [99]; Fecal Gram-negative bacteria [100]
Fluoroquinolone, QuinoloneE. coli, Citrobacter spp., Klebsiella spp. [101]
PenicillinSalmonella spp. [102]; Serratia spp. [103]
TetracyclineE. coli; Citrobacter spp.; Klebsiella spp. [104]; E. faecium [105]
TeicoplaninSalmonella spp. [102]
Sulphonamide; Cephalosporin; MacrolideE. coli; Citrobacter spp.; Enterobacter spp.; Klebsiella spp.; Proteus spp. [106]
CephalosporinE. coli [107]; Citrobacter spp.; Enterobacter spp.; Klebsiella spp.; Proteus spp. [106]
AminoglycosideE. coli; Citrobacter spp.; Enterobacter spp.; Klebsiella spp.; Salmonella spp.; P. aeruginosa [108]
VancomycinE. faecium [100]
ZnDue to affinity of Zn to thiol group, the Zn metal is toxic to bacteria, retards glycolysis, transmembrane proton translocation and acid tolerance in the bacterial cell. Also decreases the biomass causing growth inhibition.Resistance to Zn is found in Gram negative and gram positive bacteria and resistance is mainly via czrC geneNorfloxacin, Augmentin, Gentamicin, AmpicillinBacteria isolated from the soil of Kenya [109]
CarbapenemPseudomonas aeruginosa [94]
Penicillin, TeicoplaninSalmonella spp. [102]
Fluoroquinolone, quinoloneE. coli; Citrobacter spp. [101]
MethicillinStaphylococcus aureus [110]
CrDue to strong oxidizing potential the metal Cr damages the cells of the microbes,inhibits the oxygen uptake, growth and elongation of lag phase.Cr in microbes affects basal energy metabolism, protein oxidative stress protection, DNA repair, detoxification of enzymes, efflux pumps, homeoostasisTetracycline, CarbapenemSoil bacteria of Scotland [90]
PenicillinEnterobacter spp. [97]
Cephalosporin, TetracyclineP. aeruginosa [97]
Nitrofuran, TeicoplaninSalmonella spp. [102]
Quinolone, VancomycinE. coli [111]
SulfonamideKlebsiella spp. [112]
CdCd in bacteria denatures the protein, interacts with calcium metabolism, damages the cell membrane, hinders cell division and transcription, and also affects the nucleic acid.Resistance to Cd in Gram negative bacteria affects Czc and Ncc genes and encoding dsbA gene needed for disulphite formation while in Gram positive bacteria resistance to Cd is with the CdA pumpAugmentin, AmpicillinBacteria isolated from the soil of Kenya [109]
AmpicillinBacteria isolated from ship [96]
Fluoroquinolone, QuinoloneE. coli; Citrobacter spp.; Klebsiella spp. [101]
Penicillin, TetracyclineP. aeruginosa [113]
Amphenicol, Cephalosporin Methicillin, Sulfonamide, AminoglycosideE. coli; Citrobacter spp.; Klebsiella spp. [112]
MacrolideE. faecium [98]
PbLead induces mutagenicity, inhibits enzyme activities and transcription. Pb in the bacteria destroys the nucleic acid.Resistance mechanism is due to adsorption of lead by extracellular polysaccharides, cell exclusion and ion efflux to the cell exterior.AminoglycosideCitrobacter spp. [104]
Macrolide, QuinoloneE. coli [111]
PenicillinEnterobacter spp. [107]
TeicoplaninE. faecium [105]
QuinoloneKlebsiella spp. [106]
SulphonamideProteus spp. [106]
VancomycinEnterobacter spp.; Klebsiella spp. [106]
AmphenicolSalmonella Spp. [114]
FluoroquinoloneP. aeruginosa [115]
TetracyclineShigella spp. [116]
NiNi replaces the essential metals from metalloprotein. The activity of the enzymes is retarded as Ni binds the catalytic site of the enzyme. Ni causes oxidative stress which results in the enhanced DNA damage, protein impairment, and lipid peroxidation.The resistance mechanism is due to energy-dependent Ni efflux pump induced by cnr which is promoted by a chemo-osmotic proton-antiporter system.QuinoloneE. coli [111]
TetracyclineSoil bacteria of Scotland [90]
PenicillinSalmonella spp. [102]
AmphenicolE. faecium [105]
SulfonamideCitrobacter spp. [112]
AsDisrupts enzymatic functions in the cell and interferes in the phosphate uptake and utilization.Activation of efflux pumps due to cross resistance between arsenic and antibiotics is the main mechanismPenicillinE. coli [107]
SulfonamideSalmonella spp. [117]
TetracyclineE. coli [118]
AmphenicolSalmonella spp. [119]
CoProduces non B12 cobalt protein.The gene Czc, affects the inner and outer membranes and removes the cobalt from the cytoplasmAminoglycosideCitrobacter spp. [106]
MacrolideEnterobacter spp. [120]
PenicillinSalmonella spp. [102]
QuinoloneE. coli [111]
SulfamethoxazoleP. aeruginosa [121]
VancomycinEnterobacter spp.; Klebsiella spp. [106]

Table 4.

Impact of potentially toxic metals on some bacterial strain resistant to antibiotics.


8. Toxicity of potentially toxic metals

World Health Organization has reported that globally in the year 2015 approximately 8.8 million deaths were due to cancer, presence of potentially toxic metals beyond permissible limits within the environment is one among the main factors of the death because the endocrine system is disrupted by these metals. When the food or drinking water containing potentially toxic metals beyond their maximum tolerance concentration is ingested, the metabolism of living cells in the body is negatively affected [122]. The immune and hematopoietic systems in human and animals also are adversely affected on exposure to the mixtures of those metals [122]. Li et al. [123] reported that the main cause for human bone diseases is the presence of the potentially toxic metals beyond their permissible limit within the aquatic environment. Potentially toxic metals Pb, Hg, Cd, As, and Cr in living cells causes cytotoxicity [124] and oxidative stress [124], leading to the damages of antioxidants, enzyme inhibition, apoptosis (programmed cell death), loss of DNA repair mechanism, protein dysfunction, and damage to lipid peroxidase and of the membrane.

8.1 Cadmium

Cadmium in human causes Itai-Itai disease, liver/kidney lesions, hepato-colic effects [125], carcinoma, prostatic adenocarcinoma, osteoporosis, hypertension, disorder, and kidney lesions including enlargement, nuclear, and mitochondrial damages, and histological changes; decreased antioxidant power of kidney also disrupts mineral balance within the body, causing dysfunctions of sexual glands and skeltel diseases. A psychomotor function of the brain is bogged down in the presence of Cd. The toxicity of cadmium to cell is because Cd can displace vitamin C and E from their metabolically active sites, decrease in absorption of calcium by intestine and enhanced dissolution of bone calcium causing disorder in the normal bone metabolism processes. Cd an endocrine disrupter causes neuro-developmental toxicity. Toxicity of Cd in fishes includes immune suppression and immune dysfunction.

8.2 Lead

Out of about four million tons of lead used per annum globally about three million tons is discharged within the environment. In the physical body, 90–95% of the intruding lead is accumulated within the bones which combine with bone minerals and organic matter causes rise in the blood lead level. Accumulation of lead within the bones affects the acid-base equilibrium causing calcium deficiency. Pb within the body lowers the active vitamin D3 level and parathyroid level within the plasma affecting somatic cell function viz., decrease in the secretion of γ-carboxyglutamic acid containing protein. Clinical studies have shown that if the extent of Pb in the drinking water is 50 μg/L, the blood lead level within the human is going to be about 30 μg/L; if the extent increased further, the lead level within the breast fed babies are enhanced causing hindrance within the bone development. If the blood lead level in children exceeds 75 μg/L, it causes coma, convulsions, and eventually death. Pb also affects central systema nervosum, renal, cardiovascular, neurological, and musculoskeletal systems. Pb influences the heme synthesizing enzymes by replacing Zn within the heme synthesis. Lead disrupts biosynthesis of hemoglobin, metabolism of Fe, Zn, and Cu, and of vitamin D within the body, and also causes cognitive impairment. Pb in physical body also acts as nephrotoxicants. Lead in fish’s body also affects immune system [122]. Long-term exposure to the low concentration of a mix of Cd, As, and Pb in human and other animal cause hepatotoxic (damage to the liver) effects [123].

8.3 Chromium

The annual output of Cr globally is approximately 7.5 million tons. The secretion of Collagen-Type I which helps in the bone fracture healing is suppressed in the presence of chromium ion. Chromium in human causes nose ulcers, asthma, DNA damage, hemolysis, damage to liver, kidney, and carcinoma.

8.4 Arsenic

Smith et al. [126] after their research studies reported that if the person get 50 μg/L of arsenic (daily) then 13 out of 1000 individuals will suffer with lung, liver, kidney or bladder cancer. Skin lesions are by the uptake of 0.0012 mg/kg/day of arsenic. Bhattacharya et al. [125] found that low concentration of arsenic for long period damages liver in human and other animals. Enlargement of kidney, nuclear, and mitochondrial damages, histological changes, and decreased antioxidant power of kidney is additionally caused by arsenic in human. Arsenic also causes neurotoxic effects in human with the assembly of the reactive oxygen species which incorporates death of neuronal cells, cognitive dysfunction, and Alzheimer’s disease. Cognitive impairment, deafness, hypertension, anemia dementia, hematemesis, and bladder cancer also is caused by As. The prolonged exposure to arsenic also affects central systema nervosum.

8.5 Mercury

Mercury is the third top hazardous substance. Aquatic organisms convert inorganic mercury to methyl mercury which inactivates Na+/K+ ATPase. Hg with the production of reactive oxygen causes neurotoxic effects in human including death of neuronal cells, cognitive dysfunction, and Alzheimer’s disease. As Hg is an endocrine disrupter, during pregnancy exposure to metal causes long-term damages to new born as mercury disrupts the influences the maternal-fetal balance. Minamata disease, renal toxicity, skin, nose irritation, damage to central systema nervosum, hearing speech, and visual disorders are another health risks to human.

8.6 Copper

Copper an integral part of several enzymes in small amount (0.9 mg daily uptake) is an essential metal for animals and plants. Deficiency of copper in human causes anemia, a low number of leucocytes, defects in animal tissue, and osteoporosis in infants. The copper within the body beyond its permissible limit causes hematemesis, jaundice, melena, damage to central nervous system, liver, and kidney problems. Wilson’s disease a genetic disease is additionally caused by copper.

8.7 Nickel

Nickel, a natural occurring metal, exists in a number of mineral forms and is an ingredient of chocolate, steel, and other metal products, pigments, valves and of batteries. Excess uptake of nickel by human causes asthma, pneumonia, allergies, heart disorder, skin rashes, and miscarriage. Chances of development of carcinoma, nose cancer, larynx cancer, and prostatic adenocarcinoma also are enhanced.

8.8 Cobalt

Cobalt is an essential metal for the life as it is the integral part of vitamin B12 (cobalamin). Human when exposed to the higher concentration of cobalt causes decreased pulmonary function, asthma, interstitial lung disease, wheezing, and dyspnoea and reduces pulmonary function. Respiratory tract hyperplasia, pulmonary fibrosis, increase in number of red blood cells, emphysema, paralysis of the systema nervosum, seizures, growth retardation, and thyroid deficiency are diseases occurs in human at a really high concentration of the metal.

8.9 Zinc

As zinc plays a crucial role in number of metallo enzymes viz., dehydrogenase, alkaline phosphatase, carbonic anhydrase, leucine amino peptidase, superoxide dismutase, and deoxyribosenucleic acid (DNA) and ribosenucleic acid (RNA) polymerase is an essential metal in humans and animals. Over exposures to zinc in human causes dry or pharyngitis, chest tightness, headache, increased indices of pulmonary inflammation, nausea, decrease in the activity of copper metallo enzyme, decreased HDL-cholesterol level, immuno toxicity, and gastrointestinal effects.


9. Conclusions

  • Contamination of ground and surface water by potentially toxic metals is a worldwide problem.

  • The major route of the groundwater and aquatic contamination by potentially toxic metals are the leaching from toxic industrial waste dumps, municipal landfills, and leaching of agricultural chemicals from soils into the upper aquifers.

  • Potentially toxic metals contaminated vegetables and fruits; fishes, seafood, and drinking water are the most sources of the ingestion of those metals by the citizenry.

  • A number of biological and biochemical processes are disrupted in the physical body by accumulation of those metals. These metals also cause developmental abnormalities in the children.

  • These potentially toxic metals promote the spread of antibiotic resistant genes which causes the ineffectiveness of broad- spectrum antibiotics.



No original data is utilized in this review; all information is accessed from published work.


  1. 1. Li L, Yang X. The essential element manganese, oxidative stress, and metabolic diseases: Links and interactions. Oxidative Medicine and Cellular Longevity. 2018;2018:7580707. DOI: 10.1155/2018/.580707
  2. 2. Zwolak A, Sarzyńska M, Szpyrka E, Stawarczyk K. Sources of soil pollution by heavy metals and their accumulation in vegetables: A review. Water, Air, and Soil Pollution. 2019;230:164. DOI: 10.1007/s11270-019-4221-y
  3. 3. Ali H, Khan E, Ilahi I. Environmental chemistry and ecotoxicology of hazardous heavy metals: Environmental persistence, toxicity, and bioaccumulation. Journal of Chemistry. 2019;2019:6730305. DOI: 10.1155/2019/6730305
  4. 4. Jiao Z, Li H, Song M, Wang L. Ecological risk assessment of heavy metals in water and sediment of the Pearl River estuary, China. Materials Science and Engineering. 2018;394:052055. DOI: 10.1088/1757-899X/394/5/052055
  5. 5. Nizami G, Rehman S. Assessment of heavy metals and their effects on quality of water of rivers of Uttar Pradesh, India: A review. Environmental Toxicology and Chemistry. 2018;2:65-71
  6. 6. Paul D. Research on heavy metal pollution of river ganga: A review. Annals of Agrarian Science. 2017;15:278-286
  7. 7. Toth G, Hermann T, Da Silva MR, Montanerella L. Heavy metals in agricultural soils of the European Union with implications for food safety. Environment International. 2016;88:299-309
  8. 8. Sharma B, Sarkar A, Singh P, Singh RP. Agricultural utilization of biosolids: A review on potential effects on soil and plant grown. Waste Management. 2017;64:117-132. DOI: 10.1016/j.wasman.2017.03.002
  9. 9. Galitskaya IV, Rama Mohan K, Keshav Krishna A, Batral GI, Eremina ON, Putilina VS, et al. Assessment of soil and groundwater contamination by heavy metals and metalloids in Russian and Indian megacities. Procedia Earth and Planetary Science. 2017;17:674-677
  10. 10. Rezania S, Taib SM, Md Din MF, Dahalan FA, Kamyab H. Comprehensive review on phytotechnology: Heavy metals removal by diverse aquatic plants species from wastewater. Journal of Hazardous Materials. 2016;318:587-599
  11. 11. Gbogbo F, Arthur-Yartel A, Bondzie JA, Dorleku WP, Dadzie S, Kwansa-Bentum B, et al. Risk of heavy metal ingestion from the consumption of two commercially valuable species of fish from the fresh and coastal waters of Ghana. PLoS One. 2018;13(3):e0194682. DOI: 10.1371/journal.pone.0194682
  12. 12. Ahmed MK, Parvin E, Islam MM, Akter MS, Khan S, Al-Mamun MH. Lead- and cadmium-induced histopathological changes in gill, kidney and liver tissue of freshwater climbing perch Anabas testudineus (Bloch, 1792). Chemistry and Ecology. 2014;30:532-540
  13. 13. Wei J, Duan M, Li Y, et al. Concentration and pollution assessment of heavy metals within surface sediments of the Raohe Basin, China. The Scientific Reporters. 2019;9:13100. DOI: 10.1038/s 415 98-019-49724-7
  14. 14. Kulkarni HV, Mladenov N, Datta S, Chatterjee D. Influence of monsoonal recharge on arsenic and dissolved organic matter in the Holocene and Pleistocene aquifers of the Bengal Basin. Science of the Total Environment. 2018;637–638:588-599. DOI: 10.1016/j.scitonev.2018.05.009
  15. 15. Fatima S, Hussain I, Rasool A, Xiao T, Farooqi A. Comparison of two alluvial aquifers shows the probable role of river sediments on the release of arsenic in the groundwater of district Vehari, Punjab, Pakistan. Environmental Earth Sciences. 2018;77:382. DOI: 10.1007/s12665-018-7542-z
  16. 16. Jiang Z, Li P, Tu J, Wei D, Zhang R, Wang Y, et al. Arsenic in geothermal systems of Tengchong, China: Potential contamination on freshwater resources. International Biodeterioration & Biodegradation. 2018;128:28-35. DOI: 10.1016/j.ibiod.2016.05.013
  17. 17. Bansal OP. Groundwater quality of Aligarh district of Uttar Pradesh, India, a 11 year study. Pollution Research. 2008;27:721-724
  18. 18. Bansal OP. Heavy metals in soil, plants as influenced by irrigation with sewage effluents: A 20 year study. Journal of Indian Association for Environmental Management. 2008;35:143-148
  19. 19. Chika OC, Prince EA. Comparative assessment of trace and heavy metals in available drinking water from different sources in the Centre of Lagos and off town (Ikorodu LGA) of Lagos state, Nigeria. Advanced Journal of Chemistry, Section A. 2020;3(1):94-104
  20. 20. Kwaya MY, Hamidu H, Kachalla M, Abdullahi IM. Preliminary ground and surface water resources trace elements concentration, toxicity and statistical evaluation in part of Yobe State, North Eastern Nigeria. Geosciences. 2017;7:117-128. DOI: 10.5923/j.geo.20170704.02
  21. 21. Salman SA, Zeid SAM, Seleem EMM, Abdel-Hafiz MA. Soil characterization and heavy metal pollution assessment in Orabi farms, El Obour, Egypt. Bulletin of the National Research Centre. 2019;43:42. DOI: 10.1186/s42269-019-0082-1
  22. 22. Aloke C, Uzuegbu IE, Ogbu PN, Ugwuja EL, Orinya OF, Obasi IO. Comparative assessment of heavy metals in drinking water sources from Enyigba Community in Abakaliki Local Government Area, Ebonyi state, Nigeria. African Journal of Environmental Science and Technology. 2019;13:149-154
  23. 23. Kacholi DS, Sahu M. Levels and health risk assessment of heavy metals in soil, water, and vegetables of Dar es Salaam, Tanzania. Journal of Chemistry. 2018;2018:1402674. DOI: 10.1155/2018/1402674
  24. 24. Chakraborti D, Singh S, Rahman M, Dutta R, Mukherjee S, Pati S, et al. Groundwater arsenic contamination in the Ganga River basin: A future health danger. International Journal of Environmental Research and Public Health. 2018;15:180. DOI: 10.3390/ijerph15020180
  25. 25. Ramachandran A, Krishnamurthy RR. Jayaprakash M, Balasubramanian M: Concentration of heavy metal in surface water and groundwater Adyar River basin, Chennai, Tamilnadu, India. IOSR Journal of Applied Geology and Geophysics. 2018;6:29-35
  26. 26. Sridhar SGD, Sakthivel AM, Sangunathan U, Jenefer S, Mohamed Rafik M, Kanagaraj G. Heavy metal concentration in groundwater from Besant Nagar to Sathankuppam, South Chennai, Tamil Nadu, India. Applied Water Science. 2017;7:4651-4662
  27. 27. Rana A, Bhardwaj SK, Thakur M, Verma S. Assessment of heavy metals in surface and ground water sources under different land uses in mid-hills of Himachal Pradesh. International Journal of Bio-resource and Stress Management. 2016;7(3):461-465. DOI: 10.5958/0976-4038.2016.00074.9
  28. 28. Godwin AOM, Chinenye NG. Bioaccumulation of selected heavy metals in water, sediment and blue crab (Callinectes amnicola) from Bodo Creek, Niger Delta, Nigeria. Journal of Fisheries Science. 2016;10(3):77-83
  29. 29. Wasike PW, Nawiri MP, Wanyonyi AA. Levels of heavy metals (Pb, Mn, Cu and Cd) in water from river Kuywa and the adjacent wells. Environment and Ecology Research. 2019;7:135-138. DOI: 10.13189/eer.2019.070303
  30. 30. Kwaya MY, Hamidu H, Mohammed I, Abdulmumini N, Adamu IH, Grema M, et al. Heavy metals pollution indices and multivariate statistical evaluation of groundwater quality of Maru town and environs. Journal of Materials and Environmental Science. 2019;10:32-44
  31. 31. Deda A, Alushllari M, Mico S. Measurement of heavy metals in ground water. AIP Conference Proceedings. 2019;2109:100001-100004. DOI:10.1063/1.5110136
  32. 32. Vatandoosta M, Naghipoura D, Omidia S, Ashrafia SD. Survey and mapping of heavy metals in groundwater resources around the region of the Anzali international wetland; a dataset. Data in Brief. 2018;18:463-469
  33. 33. Singh UK, Ramanathan AL, Subramanian V. Groundwater chemistry and human health risk assessment in the mining region of east Singhbhum Jharkhand, India. Chemosphere. 2018;204:501-513. DOI: 10.1016/j.chemosphere.2018.04.060
  34. 34. Withanachchi SS, Ghambashidze G, Kunchulia I, Urushadze T, Ploeger A. Water quality in surface water: A preliminary assessment of heavy metal contamination of the Mashavera River, Georgia. International Journal of Environmental Research and Public Health. 2018;15(4):621. DOI: 10.3390/ijerph15040621
  35. 35. Khan MI, Khisroon M, Khan A, Gulfam N, Siraj M, Zaidi F, et al. Bioaccumulation of heavy metals in water, sediments, and tissues and their histopathological effects on Anodonta cygnea (Linea, 1876) in Kabul River, Khyber Pakhtunkhwa, Pakistan. BioMed Research International. 2018;2018:1910274. DOI: 10.1155/2018/1910274
  36. 36. Decena SCP, Arguilles MS, Robel LL. Assessing heavy metal contamination in surface sediments in an Urban River in the Philippines. Polish Journal of Environmental Studies. 2018;27(5):1983-1995. DOI: 10.15244/pjoes/75204
  37. 37. Neha Kumar D, Shukla P, Kumar S, Bauddh K, Tiwari J, Dwivedi N, et al. Metal distribution in the sediments, water and naturally occurring macrophytes in the river Gomti, Lucknow, Uttar Pradesh, India. Current Science. 2017;113:1578-1585
  38. 38. Singh H, Pandey R, Singh SK, Shukla DN. Assessment of heavy metal contamination in the sediment of the river Ghaghara, a major tributary of the river ganga in northern India. Applied Water Science. 2017;7:4133-4149
  39. 39. Pandey J, Singh R. Heavy metals in sediments of Ganga River: Up- and downstream urban influences. Applied Water Science. 2017;7:1669-1678
  40. 40. Khan MZH, Hasan MR, Khan M, Aktar S, Fatema K. Distribution of heavy metals in surface sediments of the Bay of Bengal coast. Journal of Toxicology. 2017;2017:9235764. DOI: 10.1155/2017/9235764
  41. 41. Uddin MN, Hasan MK, Dhar PK. Contamination status of heavy metals in vegetables and soil in Satkhira, Bangladesh. Journal of Materials and Environmental Science. 2019;10:543-552
  42. 42. Ratul AK, Hassan M, Uddin MK, Sultana MS, Akbor MA, Ahsan MA. Potential health risk of heavy metals accumulation in vegetables irrigated with polluted river water. International Food Research Journal. 2018;25(1):329-338
  43. 43. Sultana MS, Rana S, Yamazaki S, Aono T, Yoshida S. Sofian Kanan (reviewing editor): Health risk assessment for carcinogenic and non-carcinogenic heavy metal exposures from vegetables and fruits of Bangladesh. Cogent Environmental Science. 2017;3:1. DOI: 10.1080/23311843.2017.1291107
  44. 44. Hadayat N, De Oliveria L, Silva ED, Han L, Hussain M, Liu X, et al. Assessment of trace metals in five most-consumed vegetables in the US: Conventional vs. organic. Environmental Pollution. 2018;243:292-300
  45. 45. Naghipour D, Chenari MA, Taheri N, Naghipour F, Mehrabian F, Attarchi MA, et al. The concentration data of heavy metals in vegetables of Guilan province, Iran. Data in Brief. 2018;21:1704-1708
  46. 46. Zafarzadeh A, Rahimzadeh H, Mahvi AH. Health risk assessment of heavy metals in vegetables in an endemic esophageal cancer region in Iran. Health Scope. 2018;7(3):e12340. DOI: 10.5812/jhealthscope.12340
  47. 47. Latif A, Bilal M, Asghar W, Azeem M, Ahmad IM, Abbas A, et al. Heavy metal accumulation in vegetables and assessment of their potential health risk. International Journal of Environmental Analytical Chemistry. 2018;5(1):234-245. DOI: 10.4172/2380-2391.1000234
  48. 48. Hong AH, Umaru AB. Heavy metals concentration levels in vegetables irrigated from Lake Geriyo area of Adamawa state, Nigeria. International Journal of Engineering and Science Invention. 2018;7:39-42
  49. 49. Hassoon HA. Heavy metals contamination assessment for some imported and local vegetables. Iraqi Journal of Agricultural Sciences. 2018;49(5):794-802
  50. 50. Zhou H, Yang WT, Zhou X, Liu L, Gu JF, Wang WL, et al. Accumulation of heavy metals in vegetable species planted in contaminated soils and the health risk assessment. International Journal of Environmental Research and Public Health. 2016;13:289-296. DOI: 10.3390/ijerph13030289
  51. 51. Siaka IM, Utama IMS, Manuaba IBP, Adnyana M. Heavy metals contents in the edible parts of some vegetables grown in Candi Kuning, Bali and their predicted pollution in the cultivated soil. Journal of Environment and Earth Science. 2014;4:78-83
  52. 52. Säumel I, Kotsyuk I, Hölscher M, Lenkereit C, Weber F, Kowarik I. How healthy is urban horticulture in high traffic areas? Trace metal concentrations in vegetable crops from plantings within inner city neighborhoods in Berlin, Germany. Environmental Pollution. 2012;165:124-132. DOI: 10.1016/j.envpol.2012.02.019
  53. 53. Bansal OP. Heavy metals in sewage effluent water irrigated vegetables and their potential health risks to consumers of Aligarh, India. Chemical Science Review and Letters. 2014;3:589-596
  54. 54. Kabir A, Bhuyan MS. Heavy metals in egg contents of hens (Gallus gallus domesticus) and ducks (Anas platyrhynchos) from Chittagong region. Bangladesh Journal Pollution Effective Control. 2019;7:232-237
  55. 55. Malik DS, Maurya PK. Heavy metal concentration in water, sediment, and tissues of fish species (Heteropneustis fossilis and Puntius ticto) from Kali River, India. Toxicological & Environmental Chemistry. 2014;96:1195-1206
  56. 56. Luo J, Ye Y, Gao Z, Wang W. Essential and nonessential elements in the red-crowned crane Grus japonensis of Zhalong wetland, North-Eastern China. Toxicological & Environmental Chemistry. 2014;96:1096-1105
  57. 57. Salam MA, Paul SC, Noor SNBM, Siddiqua SA, Aka TD, Wahab R, et al. Contamination profile of heavy metals in marine fish and shellfish. Global Journal of Environmental Science and Management. 2019;5:225-236
  58. 58. Kaur S, Khera KS, Kondal JK. Effect of water contaminated with heavy metals on histopathology of freshwater catfish, Clarias batrachus. International Journal of Chemical Studies. 2018;6(4):3103-3108
  59. 59. Yousafzai AM, Ullah F, Bari F, Raziq S, Riaz M, Khan K, et al. Bioaccumulation of some heavy metals: Analysis and comparison of Cyprinus carpio and Labeo rohita from Sardaryab, Khyber Pakhtunkhwa. BioMed Research International. 2017;2017:5801432. DOI: 10.1155/2017/5801432
  60. 60. Mahoob S, Kausar S, Jabeen F, Sultana S, Sultana T, Al-Ghanim KA, et al. Effect of heavy metals on liver, kidney, gills and muscles of Cyprinus carpio and Wallago attu inhabited in the Indus. Brazilian Archives of Biology and Technology. 2016;59:1-10. DOI: 10.1590/1678-4324-20161502
  61. 61. Harsimran Kaur B. Accumulation of heavy metals in fishes of freshwater [Thesis]. Department of Zoology, University of Delhi; 2016. p. 44
  62. 62. El-Batrawy OA, El-Gammal MI, Mohamadein LI, Darwish DH, El-Moselhy M. Impact assessment of some heavy metals on tilapia fish, Oreochromis niloticus, in Burullus Lake, Egypt. The Journal of Basic and Applied Zoology. 2018;79:13. DOI: 10.1186/s41936-018-0028-4
  63. 63. Ray Chaudhuri T, Dutta P, Zaman S, Mitra A. Status of edible fishes of lower gangetic delta in terms of heavy metals. International Journal of Environmental Sciences & Natural Resources. 2017;5(3):555661. DOI: 10.19080/IJESNR.2017.05.555661
  64. 64. Ibemenuga KN, Ezike F, Nwosu MC, Anyaegbunam LC, Okoye EI, Eyo JE. Bioaccumulation of some heavy metals in some organs of three selected fish of commercial importance from Niger River, Onitsha shelf, Anambra state, Nigeria. Journal of Fisheries Science. 2019;13:001-012
  65. 65. Mensoor M, Said A. Determination of heavy metals in freshwater fishes of the Tigris River in Baghdad. Fishes. 2018;3:23. DOI: 10.3390/fishes3020023
  66. 66. Ionita C, Mititelu M, Morosan E. Analysis of heavy metals and organic pollutants from some Danube River fishes. Farmácia. 2014;62(2):299-305
  67. 67. Milanov DR, Krstic M, Markovic R, Jovanovic AD, Baltic MB, Ivanovic SJ, et al. Analysis of heavy metals concentration in tissues of three different fish species included in human diet from Danube River, in the Belgrad region, Serbia. Acta Veterinaria Belgrade. 2016;66:89-102
  68. 68. Miloskovic A, Dojcinovic B, Kovacevic S, Radojkovic N, Radenkovic M, Milosevic D, et al. Spatial monitoring of heavy metals in the inland waters of Serbia: A multispecies approach based on commercial fish. Environmental Science and Pollution Research. 2016;23(10):9918-9933
  69. 69. Khillare K, Khillare YK, Wagh U. Bioaccumulation of heavy metals in freshwater fishes from Aurangabad district Maharashtra. World Journal of Pharmacy and Pharmaceutical Sciences. 2015;4:511-520
  70. 70. Mercy M, Dhanalakshmi B. Toxicological evaluation of heavy metals in tissues of freshwater fish Oreochromis niloticus collected from lakes of Coimbatore district, Tamilnadu, India. International Research Journal of Pharmacy. 2017;8(1):41-45. DOI: 10.7897/2230-8407.08018
  71. 71. Leung HM, Leung AO, Wang HS, Ma KK, Liang Y, Ho KC, et al. Assessment of heavy metals/metalloid (As, Pb, Cd, Ni, Zn, Cr, Cu, Mn) concentrations in edible fish species tissue in the Pearl River Delta (PRD), China. Marine Pollution Bulletin. 2014;78(1–2):235-245. DOI: 10.1016/j.marpolbul.2013.10.028
  72. 72. El-Moselhy KM, Othman AI, El-Azem A, El-Metwally MEA. Bioaccumulation of heavy metals in some tissues of fish in the Red Sea, Egypt. Egyptian Journal of Basic and Applied Sciences. 2014;1:97-105
  73. 73. Reyahi-Khoram M, Setayesh-Shiri F, Cheraghi M. Study of the heavy metals (Cd and Pb) content in the tissues of rainbow trouts from Hamedan Coldwater fish farms. Iranian Journal of Fisheries Sciences. 2016;15(2):858-869
  74. 74. Sulieman HMA, Suliman EAM. Appraisal of heavy metal levels in some marine organisms gathered from the Vellar and Uppanar estuaries southeast coast of Indian Ocean. Journal of Taibah University for Science. 2019;13:338-343
  75. 75. Nasyitah SN, Ahmad ZA, Khairul NM, Ley JL, Kyoung-Woong K. Bioaccumulation of heavy metals in Maricultured fish, Lates calcarifer (Barramudi), Lutjanus campechanus (red snapper) and Lutjanus griseus (Grey snapper). Chemosphere. 2018;197:318-324. DOI: 10.1016/j. chemosphere.2017.12.187
  76. 76. Younis AM, Amin HF, Alkaladi A, Mosleh YYI. Bioaccumulation of heavy metals in fish, squids and crustaceans from the Red Sea, Jeddah coast, Saudi Arabia. Open Journal of Marine Science. 2015;5:369-378. DOI: 10.4236/ojms.2015.54030
  77. 77. Bazzi AO. Heavy metal in sea water, sediment and marine organism in the gulf of Chabahar, Oman Sea. Journal of Oceanography and Marine Science. 2014;5:20-29
  78. 78. Bat L, Arici E, Sezgin M, Sahin F. Heavy metals in edible tissues of benthic organisms from Samsun coasts, south Black Sea Turkey and their potential risk to human health. Journal of Food and Health Science. 2016;2:57-66. DOI: 10.3153/JFHS16006
  79. 79. Sadeghi E, Pirsaheb M, Mohammadi M, Salti AP, Sharafi H, Mirzaei N, et al. Evaluation of cadmium and lead levels in fillet marine fish (Otolithes ruber and Lutjanus johni) from Persian gulf. Annals of Tropical Medicine & Public Health. 2017;10:1015-1018
  80. 80. Eken M, Aydin F, Turan F, Uyan A. Bioaccumulation of some heavy metals on silver-cheeked toadfish (Lagocephalus sceleratus) from Antalya Bay, Turkey. Natural and Engineering Sciences. 2017;2(3):12-21
  81. 81. Jayaprabha N, Balakrishnan S, Purusothaman S, Indira K, Srinivasan M, Anantharaman P. Bioaccumulation of heavy metals in flying fishes along southeast coast of India. International Food Research Journal. 2014;21(4):1381-1386
  82. 82. Mehouel F, Bouayad L, Hammoudi AH, Ayadi Q, Regad F. Evaluation of the heavy metals (mercury, lead, and cadmium) contamination of sardine (Sardina pilchardus) and swordfish (Xiphias gladius) fished in three Algerian coasts. Veterinary World. 2019;12(1):7-11. DOI: 10.14202/vetworld.2019.7-11
  83. 83. Bashir FA, Alhemmali EM. Analysis of some heavy metal in marine fish in muscle, liver and gill tissue in two marine fish spices from Kapar coastal waters, Malaysia. In: The Second Symposium on Theories and Applications of Basic and Biosciences. Misrata, Libya; 2015
  84. 84. Rosli MNR, Samat SB, Yasir MS, Yusof MFM. Analysis of heavy metal accumulation in fish at Terengganu coastal area, Malaysia. Sains Malaysiana. 2018;47(6):1277-1283. DOI: 10.17576/jsm-2018-4706-24
  85. 85. Olusola JO, Festus AA. Assessment of heavy metals in some marine fish species relevant to their concentration in water and sediment from coastal waters of Ondo state, Nigeria. Journal of Marine Science: Research & Development. 2015;5:163. DOI: 10.4172/2155-9910.1000163
  86. 86. Bandpei AM, Bay A, Zafarzadeh A, Hassanzadeh V. Bioaccumulation of heavy metals muscle of common carp fish (Cyprinus carpio L, 1758) from Ala gul and Alma gul wetlands of Golestan and consumption risk assessment. International Journal of Medical Research & Health Science. 2016;5:267-273
  87. 87. Tugui C, Szekeres E, Baricz A. Sources and mechanisms of combined heavy-metal and antibiotic resistance traits in bacteria. Studia Universitatis Babeș-Bolyai Biologia. 2017;LXII:101-114
  88. 88. Pal C, Asiani K, Arya S, Rensing C, Stek DJ, DGJ L, et al. Metal resistance and its association with antibiotic resistance. Advances in Microbial Physiology. 2017;70:261-301. DOI: 10.1016/bs.ampbs.2017.02.001
  89. 89. Knapp CW, Callan AC, Aitken B, Shearn R, Koenders A, Hinwood A. Relationship between antibiotic resistance genes and metals in residential soil samples from Western Australia. Environmental Science and Pollution Research International. 2017;24(3):2484-2494. DOI: 10.1007/s11356-016-7997-y
  90. 90. Knapp CW, McCluskey SM, Singh BK, Campbell CD, Hudson G, Graham DW. Antibiotic resistance gene abundances correlate with metal and geochemical conditions in archived scottish soils. PLoS One. 2011;6:e27300. DOI: 10.1371/journal.pone.0027300
  91. 91. Amachawadi RG, Scott HM, Alvarado CA, et al. Occurrence of the transferable copper resistance gene tcrB among fecal enterococci of U.S. feedlot cattle fed copper-supplemented diets. Applied and Environmental Microbiology. 2013;79:4369-4375
  92. 92. Becerra-Castro C, Machado RA, Vaz-Moreira I, Manaia CM. Assessment of copper and zinc salts as selectors of antibiotic resistance in gram-negative bacteria. The Science of the Total Environment. 2015;530-531:367-372. DOI: 10.1016/j.scitotenv.2015.05.102
  93. 93. Silverira E, Freitas AR, Antunes P, Barros M, Campos J, Coque TM, et al. Co-transfer of resistance to high concentrations of copper and first-line antibiotics among Enterococcus from different origins (humans, animals, the environment and foods) and clonal lineages. Journal of Antimicrobial Chemotherapy. 2014;69:899-906. DOI: 10.1093/jac/dkt479
  94. 94. Li H, Luo YF, Williams BJ, Blackwell TS, Xie CM. Structure and function of OprD protein in Pseudomonas aeruginosa: From antibiotic resistance to novel therapies. International Journal of Medical Microbiology. 2012;302:63-68. DOI: 10.1016/j.ijmm.2011.10.001
  95. 95. Wales AD, Davies RH. Co-selection of resistance to antibiotics, biocides and heavy metals, and its relevance to foodborne pathogens. Antibiotics. 2015;4(4):567-604
  96. 96. Kacar A, Kocyigit A. Characterization of heavy metal and antibiotic resistant bacteria isolated from Aliaga ship dismantling zone, eastern Aegean Sea, Turkey. International Journal of Environmental Research. 2013;7(4):895-902
  97. 97. Heck K, De Marco ÉG, Duarte MW, Salamoni SP, Van Der Sand S. Pattern of multiresistant to antimicrobials and heavy metal tolerance in bacteria isolated from sewage sludge samples from a composting process at a recycling plant in southern Brazil. Environmental Monitoring and Assessment. 2015;187:328. DOI: 10.1007/s10661-015-4575-6
  98. 98. Vignaroli C, Pasquaroli S, Citterio B, Di Cesare A, Mangiaterra G, Fattorini D, et al. Antibiotic and heavy metal resistance in enterococci from coastal marine sediment. Environmental Pollution. 2018;237:406-413
  99. 99. Baker-Austin C, Wright MS, Stepanauskas R, McArthur JV. Co-selection of antibiotic and metal resistance. Trends in Microbiology. 2006;14(4):176-182
  100. 100. Ji X, Shen Q, Liu F, Ma J, Xu G, Wang Y, et al. Antibiotic resistance gene abundances associated with antibiotics and heavy metals in animal manures and agricultural soils adjacent to feedlots in Shanghai, China. Journal of Hazardous Materials. 2012;235–236:178-185
  101. 101. Anssour L, Messai Y, Estepa V, Torres C, Bakour R. Characteristics of ciprofloxacin-resistant Enterobacteriaceae isolates recovered from wastewater of an Algerian hospital. Journal of Infection in Developing Countries. 2016;10(07):728-734
  102. 102. Ali NM, Mazhar SA, Mazhar B, Imtiaz A, Andleeb S. Antibacterial activity of different plant extracts and antibiotics on pathogenic bacterial isolates from wheat field water. Pakistan Journal of Pharmaceutical Sciences. 2017;30(4):1321-1325
  103. 103. Mirzaei N, Rastegari H, Kargar M. Antibiotic resistance pattern among gram negative mercury resistant bacteria isolated from contaminated environments. Jundishapur Journal of Microbiology. 2013;6(10):634-639
  104. 104. Habi S, Daba H. Plasmid incidence, antibiotic and metal resistance among enterobacteriaceae isolated from Algerian streams. Pakistan Journal of Biological Sciences. 2009;12(22):1474-1482. DOI: 10.3923/pjbs.2009.1474.1482
  105. 105. De Niederhäusern S, Bondi M, Anacarso I, Iseppi R, Sabia C, Bitonte F, et al. Antibiotics and heavy metals resistance and other biological characters in Enterococci isolated from surface water of Monte Cotugno Lake (Italy). Journal of Environmental Science and Health, Part A. 2013;48(8):939-946
  106. 106. Djouadi LN, Selama O, Abderrahmani A, Bouanane-Darenfed A, Abdellaziz L, Amziane M, et al. Multiresistant opportunistic pathogenic bacteria isolated from polluted rivers and first detection of nontuberculous mycobacteria in the Algerian aquatic environment. Journal of Water and Health. 2017;15(4):566-579
  107. 107. Verma T, Ramteke PW, Garg SK. Occurrence of chromium resistant thermotolerant coliforms in tannery effluent. Indian Journal of Experimental Biology. 2004;42(11):1112-1116
  108. 108. Martins VV, Zanetti MOB, Pitondo-Silva A, Stehling EG. Aquatic environments polluted with antibiotics and heavy metals: A human health hazard. Environmental Science and Pollution Research International. 2014;21(9):5873-5878
  109. 109. Budambula NML, Kinyua DM. Antibiotic resistance of metal tolerant bacteria isolated from soil in Juja, Kenya. In: Conference Paper: JKUAT Scientific, Technological and Industrialization Conference November 2013. Nairobi; 2013
  110. 110. Cavaco LM, Hasman H, Stegger M, Andersen PA, Skov R, Fluit AC, et al. Cloning and occurrence of czrC, a gene conferring cadmium and zinc resistance in methicillin-resistant Staphylococcus aureus CC398 isolates. Antimicrobial Agents and Chemotherapy. 2010;54(9):3605-3608. DOI: 10.1128/AAC.00058-1
  111. 111. Abskharon RN, Hassan SH, Gad El-Rab SM, Shoreit AA. Heavy metal resistant of E. coli isolated from wastewater sites in Assiut City, Egypt. Bulletin of Environmental Contamination and Toxicology. 2008;81(3):309-315
  112. 112. Sepahy AA, Sharifian S, Zolfaghari MR, Dermany MK, Rashedi H. Study on heavy metal resistant fecal coliforms isolated from industrial, urban wastewater in Arak, Iran. International Journal of Environmental Research. 2015;9(4):1217-1224
  113. 113. Deredjian A, Colinon C, Brothier E, Favre-Bonte S, Cournoyer B, Nazaret S. Antibiotic and metal resistance among hospital and outdoor strains of Pseudomonas aeruginosa. Research in Microbiology. 2011;162(7):689-700. DOI: 10.1016/j.resmic.2011.06.007
  114. 114. Mourão J, Marçal S, Ramos P, Campos J, Machado J, Peixe L, et al. Tolerance to multiple metal stressors in emerging non-typhoidal MDR Salmonella serotypes: A relevant role for copper in anaerobic conditions. The Journal of Antimicrobial Chemotherapy. 2016;71(8):2147-2157
  115. 115. Soltan MES. Isolation and characterization of antibiotic and heavy metal-resistant Pseudomonas aeruginosa from different polluted waters in Sohag district, Egypt. Journal of Microbiology and Biotechnology. 2001;11(1):50-55
  116. 116. Manegabe BJ, Marie-Médiatrice NK, Barr Dewar J, Christian SB. Antibiotic resistance and tolerance to heavy metals demonstrated by environmental pathogenic bacteria isolated from the Kahwa River, Bukavu town, Democratic Republic of the Congo. International Journal of Environmental Studies. 2017;74(2):290-302
  117. 117. Sandegren L, Linkevicius M, Lytsy B, Melhus A, Andersson DI. Transfer of an Escherichia coli ST131 multiresistance cassette has created a Klebsiella pneumoniae-specific plasmid associated with a major nosocomial outbreak. Journal of Antimicrobial Chemotherapy. 2012;67:74-83. DOI: 10.1093/jac/dkr40.5
  118. 118. Gilmour MW, Thomson NR, Sanders M, Parkhill J, Taylor DE. The complete nucleotide sequence of the resistance plasmid R478: Defining the backbone components of incompatibility group H conjugative plasmids through comparative genomics. Plasmid. 2004;52:182-202
  119. 119. Seginkova Z, Kralikova K. Monitoring the contemporary resistance of Escherichia coli and Salmonella Sp. strains isolated from aquatic environment to antibiotics and ions of heavy-metals. Ekologia Bratislava. 1993;12(1):111-118
  120. 120. Hu Q, Dou MN, Qi HY, Xie XM, Zhuang GQ, Yang M. Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE. Journal of Environmental Sciences. 2007;19(9):1114-1119
  121. 121. Oyetibo GO, Ilori MO, Adebusoye SA, Obayori OS, Amund OO. Bacteria with dual resistance to elevated concentrations of heavy metals and antibiotics in Nigerian contaminated systems. Environmental Monitoring and Assessment. 2010;168(1–4):305-314
  122. 122. Ojedokun AT, Bello OS. Sequestering heavy metals from wastewater using cow dung. Water Resources and Industry. 2016;13:7-13
  123. 123. Li JJ, Li-Na P, Shan W, Meng-Da Z. Advances in the effect of heavy metals in aquatic environment on the health risks for bone. Earth and Environmental Science. 2018;186:012057. DOI: 10.1088/1755-1315/186/3/012057
  124. 124. Hernández-García A, Romero D, Gómez-Ramírez P, María-Mojica P, Martínez-López E, García-Fernández AJ. In vitro evaluation of cell death induced by cadmium, lead and their binary mixtures on erythrocytes of common buzzard (Buteo buteo). Toxicology In Vitro. 2014;28:300-306. DOI: 10.1016/j.tiv.2013.11.005
  125. 125. Bhattacharya PT, Misra SR, Mohsina Hussain M. Nutritional aspects of essential trace elements in oral health and disease: An extensive review. Scientifica. 2016;2016:5464373. DOI: 10.1155/2016/5464373
  126. 126. Smith AH, Lingas EO, Rahman M. Contamination of drinking-water by arsenic in Bangladesh: A public health emergency. Bulletin of the World Health Organization. 2000;78(9):1093-1103

Written By

Om Prakash Bansal

Submitted: October 1st, 2019 Reviewed: March 16th, 2020 Published: May 4th, 2020