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Barely three months into the new year and we are happy to announce a monumental milestone reached - 150 million downloads.
\n\nThis achievement solidifies IntechOpen’s place as a pioneer in Open Access publishing and the home to some of the most relevant scientific research available through Open Access.
\n\nWe are so proud to have worked with so many bright minds throughout the years who have helped us spread knowledge through the power of Open Access and we look forward to continuing to support some of the greatest thinkers of our day.
\n\nThank you for making IntechOpen your place of learning, sharing, and discovery, and here’s to 150 million more!
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Evolutionary algorithm use has been steadily increasing in the number of published papers corresponding to an increasing number of applications over the past 20 years [1, 2, 3, 4, 5, 6, 7, 8]. Originating as an alternative to traditional mathematical optimization techniques, the techniques now span across almost every discipline to include data compression, traveling salesmen, image processing, and more importantly for spacecraft: control theory [9, 10, 11, 12, 13], system identification [14, 15, 16, 17, 18, 19], and trajectory optimization [20, 21, 22, 23, 24, 25]. Randomly searching a solution space to perform a global optimization routine can be computationally expensive and time consuming. Traditional methods such as stochastic parallel gradient decent, newton’s method, and quadratic programming [26] are mathematical methods which rely mainly on the “steepness” of the gradients, or of the corresponding derivatives to follow the solution set to a zero-crossing gradient value and a potential optimum value. These methods are not easily implemented in discontinuous, or highly non-linear systems with a time-dependence such as in trajectory generation, and system identification of complex systems. One can perform a systematic, or random, search across an entire solution space, but the complex nature of some applications can limit the optimization to solution sets of only those within a small parameter space. Performing an exhausting search across an entire solution space can be considered the “brute force” method where the routine tries every single possible solution until the optimization criteria are met. For systems with a large parameter space with many variables, the computational cost might be too burdensome to arrive at a solution in a timely manner, and is certainly not relevant for a real-time application outside of a few cherry-picked examples. However, if a problem can be defined in an approachable way, evolutionary algorithms can provide a simpler and quicker way to find a viable solution. Due to the nature of these derivative free metaheuristic random search algorithms, the global optima may not be found but a suitable local optima that meets the optimization criteria can.
\nThe most prominent evolutionary algorithm is the genetic algorithm officially introduced by John Holland in his 1975 book titled “Adaptation in Natural and Artificial Systems” [27] and its primary variants involving the concepts of chromosomes, elitism, parallel populations [28, 29, 30], and adaptation [31, 32, 33] which are derived from the concept of Darwinian evolution of animal species across many generations, also known as natural selection. Genetic Algorithms will be discussed more thoroughly in Section 2. The sister approach to natural selection based evolutionary algorithms are social-evolutionary algorithms also known as swarm intelligence which will be discussed in Section 3. Swarm intelligence is also a derivative free metaheuristic random search algorithm but with a slight modification on both selection criteria and on the definitions that spawn the “evolution”. Swarm intelligence optimization algorithms for astronautical applications can be sub-categorized into particle optimization and combinatorics. Particle optimization includes particle swarm optimization [34], firefly algorithm [35], and cuckoo search algorithms [36] which focus on a large parameter space with a correspondingly large solution space that may be impossible to evaluate with traditionally exhaustive optimization routines. The artificial bee colony optimization [37], and ant colony optimization [38] algorithms are designed for a smaller investigation swarm but can successfully navigate a problem defined as an infinite set of combinations such as the commonly referred to problem of a traveling salesman visiting a large number of cities.
\nGenetic algorithms have been a staple of heuristic artificial intelligence approaches since its inception in the 1960s and later more formally introduced by John Holland in 1975 [27]. In the 1990s this kind of random search global optimization routine became more mainstream through the use of greatly increased processing speeds brought on by the personal computers any more importantly GPUs. Just like other evolutionary algorithms, genetic algorithms rely on a very specific parameter space defining the population characteristics, parent selection, and success criteria.
\nBiesbroek presents a parametric study on the fundamental parameters within a genetic algorithm application in [39] via three cases toward spacecraft trajectory optimization. The fundamental parameters include population size, mutation probability, and cross-over probability. Figure 1 illustrates the baseline genetic algorithm structure.
\nGeneric genetic algorithm flow diagram.
The first major step is in seeding a population. Seeding a population is done by presenting the algorithm an initial starting point to grow a population from. An alternative here is to randomly generate a population. A Gaussian distribution is one common approach. The parent population size is generally dependent on how many parameters are to be optimized via GA.
\nHolland described the genetic algorithm as being comprised of building blocks [27], which was later rederived by Goldberg [40] who related the population size with the quality of decisions and predicted that for an initial population of
where
Looking again at Figure 1, the next step is to create children. Children are the result of statistical combinatorics of parents, and both of the mutation and cross-over of that parent population. The initial parent population will be evaluated and scored based on an objective function. The objective function is entirely user defined for the specific application. The objective function could be described with respect to minimizing the control effort needed to achieve a specified trajectory such as described in [51], or in minimizing the error between the actual and the desired trajectory in an attitude control scenario as presented in [52]. The objective function is the key component of any optimization and it is crucial to define it in such away as to minimize (or maximize) said function efficiently and precisely. This may seem obvious but the optimization routine will focus on the weighted parameter space defined by the objective function. If a control variable is not fully observable in the prescribed control law for a system, for example, the optimization may never converge to a viable solution set.
\nThe objective function will be used to rate the performance of each member of the parent population. The population will then be ranked based on the threshold parameter specified in the algorithm. Different variations of GAs focus on certain threshold schemes to achieve faster convergence in various applications. In the basic GA scenario, those members who performed well enough to score below the threshold value (for a minimization problem) will form the parent population for the next generation. Additionally, a random subset of the original parent population will remain unmodified. Through mutation or cross-over based on mutation probability and cross-over probability. These parameters will define the statistical probability that a member of the population will be chosen for mutation or crossover. These probabilities typically start with a higher value and continually decrease on each subsequent generation to encourage the population to converge nicely to an optimal value. If a member is chosen for
Generic genetic algorithm crossover illustration.
In the flow of the genetic algorithm framework, the algorithm is said to have “worked” when it reaches convergence. Convergence here is defined such that if the fitness of the entire population is decreasing (not counting stall generations where fitness is not improved) toward a global minimum, and that on the last generation the majority of the population has very similar fitness. An example is presented in Figure 3 which illustrates a simple problem of tailoring the input of a trajectory system utilizing the dynamics based on the forced Van der Pol equation shown in Eq. (2). In this simple control example illustrated in blue and green, some arbitrary steady state value is the input to the system. It is then converted to a desired trajectory and sent to the feedforward controller. The feedforward controller builds a desired torque which is then sent as the control signal to the system dynamics, also know as the “plant”. In an ideal situation, the equation used to determine the required control torque is perfectly understood such that the system is perfectly controllable. Here, the Van der Pol system converges to a desired state but only after through a large amount of transient states which can be detrimental to the physical stability of a mechanical system.
\nSample control flow diagram using the non-linear Van der pol equation as a system under test.
This control system breaks the desired input into three components, \n
Trajectory results using classically tuned PID feedback control on a highly non-linear system versus the results using a genetic algorithm tuned to minimize the RMS error. A 40% reduction is RMS-error is achieved. (a) Trajectory results using classically tuned PID controller, and (b) Trajectory Results using a GA tuned PID controller.
Parameter | \nValue | \nResult | \n
---|---|---|
Population size | \n200 | \nFunction call per generation | \n
Population | \n3 | \nNumber of parameters to optimize | \n
Mutation rate | \n10% | \nProbability to be selected for crossover | \n
Crossover rate | \n80% | \nProbability to be selected for crossover | \n
Lower bound a | \n[0,0,0] | \nMinimum values allowed in population | \n
Upper bound a | \n[1000,1000,1000] | \nMaximum values allowed in population | \n
Selection criteria | \n5% Elite | \nChoose the top 5% of population as parents | \n
Max Stall generations | \n20 | \nExit criteria | \n
Initial population rangea | \n[0,20] | \nInitial population bounds | \n
Genetic algorithm parameters for a highly nonlinear trajectory optimization of a Van der Pol system using PID feedback control.
Constraints to the genetic algorithm.
The reduction in RMS error is illustrated in Figure 4b. The dotted line is the desired trajectory whereas the solid line is the actual trajectory achieved within the phase plane of the system. The phase plane plots the angular position vs. the angular velocity. The phase plane plot can be used to monitor trajectory tracking when you are interested in more that one state in addition to highlighting any potential instability in the system.
\nWith this example, the rate of convergence can be illustrated and examined. Figure 5 highlights the fact that the GA implementation presented here required 8800 function calls, and 43 generations to converge within the exit criteria at 20 stall generations. Figure 5 also implies that reasonable performance was achieved after only 10 generations.
\nResults of the genetic algorithm as it steps through each generation in optimizing a PID control variables.
Let us look again at the paper presented by Biesbroek [39], they look at a parameter space of only two in the application of optimizing the trajectory of a rocket such that a maximum horizontal distance,
where
Here, \n
Another area of interest is in path planning. Jia presents a parallel evolutionary algorithm solution for real-time path planning for unmanned aerial vehicles (UAVs) in [29]. The Path planning problem for UAVs start with an initial point \n
Simple illustration of a UAV path plan with no-fly zones in gray.
Taking the path planning application one step further leads us to the interplanetary path planning regime. Gage from Stanford University presented a novel paper in 1994 on interplanetary trajectory optimization using a genetic algorithm [43]. The preliminary design space for interplanetary spacecraft missions are highly complex, discontinuous, and anything beyond a two-body problem is primarily solved numerically. This initial investigation was on the viability of using a Genetic Algorithm as part of the team’s suite of search methods toward finding viable interplanetary trajectories. It was shown that the computational need was reduced by almost four times from the more common grid-search method used up through the 1990s for designing interplanetary trajectories. The keys to the performance improvement were the constraints applied to objective functions which greatly penalized infeasible trajectories resultant from the parent-children, and to also artificially degrade the fitness of population members that were in close proximity within the GA’s search space. This paper also noted that it can be helpful to restrict “mating” between parents with a separation \n
The next major subset of evolutionary algorithms relies on the assumption of swarm intelligence and social evolution in time whereas the genetic algorithms previously discussed depends upon genetic evolution of the populations; which is metaheuristic in nature and is derived from the biological (and probabilistic) mechanisms describing the movement of swarms of birds, fish, and insects on their search for food or mates. Referring to this concept as metaheuristic means that these algorithms are high-level symbolic based approaches designed to utilize imperfect or incomplete data in order to identify or approximate a sufficient solution to the given optimization problems. Swarm intelligence algorithms are based on the simple individual actions of the swarm which can collectively be quite complex and result in self-organization, decentralization and cooperation utilizing what is also referred to as collective intelligence. The primary swarm intelligence algorithms to be discussed are particle swarm optimization (PSO), cuckoo search algorithm (CSA), firefly search algorithm (FA), ant colony optimization search algorithm (ACO), and artificial bee colony algorithm (ABC).
\nParticle swarm optimization (PSO) was first introduced by Kennedy and Eberhart in 1995 [34] as a data clustering algorithm [1], and follows a population-based evolutionary social algorithm [3] along side of what can be considered an individualized random search algorithm [54]. Figure 7 outlines the overarching procedure. Initially, the algorithm is seeded with a uniformly random distribution, which is called particles. These particles will result in many different values within the search space of the system of possibilities, and again, the individual particle performance is evaluated though an objective function just like with other optimization algorithms. In the general form of PSO, the algorithm utilizes a global topology which defines how the swarm communicates with each other. Utilizing the above mentioned communication, the swarm is aware of all other particle actions, success, and current velocity. Each particle’s position within the search space (searching for the optimum value) is calculated with a corresponding velocity as defined by Eq. (7).
\nParticle swarm optimization flow chart.
where \n
Particle swarm optimization iteration illustration.
Alternatives and modifications to the PSO algorithm can include constrained velocities such as a minimum or maximum value (such that it will not grow unbounded), local biases defined by Euclidean distance between particles to define neighborhoods in order to prevent two sets of parents from different neighborhoods to attract to each other and reduce the overall fitness, and penalties for leaving the desired search space. Additionally, PSO can be hybridized with other approaches to utilize the lower computational cost of PSO but to decrease the randomness of the search if a general solution space is already known [55].
\nThe firefly search algorithm (FA) is an interesting optimization technique based on the behavior of tropical fireflies who flash their abdomens with a bioluminescence chemical in order to both attract mates, and in some species to lure in prey such as the male of competing firefly species. With this behavior there are a few components that can lend itself toward the development of an interesting swarm optimization routine. Their light flashing pattern and intensity is also affected by their desire for mates or food, along with the distance another source is from the observing firefly. The definition on describing this pattern for what specifically prompts the firefly to signal and how they signal there light is still unknown. But taking some of these concepts, an optimization routine can be developed. With this concept, the firefly algorithm was introduced by Yang [35]. The light intensity is a function of distance through the environment, which can be described by Eq. (8).
\nwhere \n
The FA routine is shown in Figure 9. This algorithm depends on an objective function just like the previously mentioned optimization algorithms. This objective function will evaluate the fitness of the fireflies, where the fitness will also determine the light intensity value at each firefly. An initial population of fireflies is generated and evaluated through the objective function. At this point the calculation for each firefly will be calculated to evaluate the attractiveness to its nearest neighbors. The attractiveness will affect the firefly’s next step as shown in Eq. (9) [35].
\nFirefly search algorithm flow chart.
where \n
Firefly search algorithm iteration illustration.
The firefly search algorithm and its variants have been applied to trajectory optimization [56, 57, 58], control parameter optimization [59, 60], and dynamics [61, 62, 63] in what can be considered as an introductory investigation by looking for initial successes in applying the FA toward these astronautical research areas.
\nThe ant colony optimization (ACO) algorithm is another approach based off of the swarm behavior of insects, and was originally designed for combinatorial problems like the traveling salesman problem (TSP) where one tries to find the minimum path for an individual to traverse across n number of cities. In the instance of the traveling salesman, the salesman has a seemingly infinite number of combinations (but not really) to try but only wants to travel the shortest path, and to not repeat any stops along the way. The basic ant system, an earlier version of ACO, was presented by Dorigo in 1992 in his PhD thesis [38]. He presented a complex optimization algorithm based on the simultaneously simple and complex nature of foraging ants that would gain a large interest in the 1990s and later. Many variants and hybrids were presented by Dorigo and others. Most notably, offline pheromone updates, and pheromone evaporation was introduced which led to the more common ACO in 1999 [64, 65]. The concept of pheromones will be discussed shortly.
\nFigure 11 illustrates at a high-level the flow of the ant colony optimization routine. The algorithm is initiated with a given set of parameters and objective function. Next, an initial set of solutions is populated. This is the first round of traveling ants looking for an optimal solution. The given problem is defined and broken apart such that the optimization routine will look for the optimal set of these building blocks in order to minimize the objective functions, or distance in the realm of the traveling salesman. At this point the pheromone level will be calculated. The pheromone is laid out such that each ant lays the same amount of pheromone out on the path that it traverses. This pheromone makes the links between different combinatorial building blocks attractive. If more than one ant traverses a similar segment the pheromone level will increase on that path segment, with an end result of the most common path being the most attractive.
\nAnt colony optimization search algorithm flow chart.
With the most traversed paths being the most attractive, one may notice that there could be a strong potential for the algorithm to get “stuck” in a local minima. The ACO algorithm includes what is know as a local pheromone update, which means that either only the last segment of a successful path will include the pheromone, or that the end segment will be delivered a heavier weight of pheromone by the one ant who achieved the best path in the current iteration. Alternatively, the best path so far (out of all the iterations) may receive that additional pheromone instead. At this point the solution space is evaluated to see if a viable global solution was found. If not, then the current ants will go through an exponential pheromone decrease before starting the next iteration in the optimization process. This pheromone decay reduces the impact of hysteresis on the solution space and helps prevent premature convergence.
\nOn the following iteration the current ant population will then create a new set of paths to achieve a solution in the population. Each link will follow pseudorandom proportional rule, which uses a uniform distribution probability weighted by the segment pheromone values to decide on each link in the path. Once all ants create the new population of solutions another iteration of pheromone decisions will follow. This entire process will continue until convergence criteria are met.
\nAnt colony optimization has been successfully used for problem sets that can be discretized into a combinatory problem such as in path planning [66, 67, 68], trajectory optimization [21, 69], and even in spacecraft load bearing [70].
\nArtificial bee colony (ABC) optimization is a derivative of both the bees system presented in 1997 by Sato and Hagiwara and the bee colony optimization by Teodorovic and Dell in 2005 [4], and was introduced later in 2005 by Karaboga [37]. The underlying algorithm flow chart is illustrated in Figure 12 and involves three types of bees: onlookers, employed bees, and scouts. At the start of the algorithm, the routine parameters are initialized, and an initial population of food sources is generated via a uniform random distribution across the solution space. The population of food sources is discovered by employed bees and the quality of the food source is evaluated via an application specific fitness function. The employed bees then randomly search for a new food source, and if that food source is of better quality, then it becomes the primary food source. If not, then the new food source is abandoned. This is called a greedy search. Meanwhile, the onlooker bees observe the actions of the employed bees, and observe their communication dance through the lens of a randomly distributed variable. This represents the decision tree on which employed bee an onlooker will follow, or if it will create a new search. If the onlooker searches for a new food source, it will choose based on a random recombination of two solutions nearby. When a food source is not picked up by the onlookers when they transition to the employed bees, that food source is now considered to be abandoned and will not be a part of the known current solution space. The next step is to repeat the search for new sources, a greedy selection, and the corresponding employed bee dance to randomly attract an onlooker to the known food source.
\nArtificial bee colony algorithm flow chart.
Similar to the ant colony optimization algorithm, the artificial bee colony algorithm is primarily designed for a combinatorics based problem where the potential solution can be discretized into an array of building blocks that can be rearranged and mutated to find an optimum solution. Additionally, the ABC algorithm has bee used for trajectory optimization [71], parameter optimization [72, 73], and remote sensing applications [74, 75].
\nAnother metaheuristic search algorithm is introduced with the cuckoo search algorithm (CSA). This approach mimics the parasitic brood behavior in certain species of the cuckoo bird. This type of bird has a fascinatingly aggressive reproduction strategy which is the heart of the CSA. Quite a few species of cuckoos participate in the obligate brood parasitism which means that they will lay their eggs in the nests of other birds [36]. The key to the success of the individual cuckoo is dependent on the cuckoo’s ability to produce an egg that is able to mimic, or approximate, the host nest eggs such that the host nest mother bird can not distinguish the cuckoo egg from her own. The cuckoo egg will hatch before the host eggs, and ensure dominance in the nest and thus prolong their survival.
\nTaking this concept to an optimization algorithm it can be illustrated as seen in Figure 13. Step one is to initialize the algorithm and generate a population of host nests. Each host nest has a single potential solution to be compared against. The next step is to evaluate the initial population of nests with the defined fitness function. Once the fitness function is evaluated for each cuckoo egg, the next step is for the cuckoo to take flight via a classical Lvy flight path [36]. This is a type of step pattern is a heavy-tailed random walk similar to that of a fruit fly, which are observed to jolt out in a straight direction then randomly turn a sharp turn at a random angle. The desired behavior is described in Eq. (10).
\nCuckoo-search algorithm flow chart.
where \n
Cuckoo-search algorithm iteration illustration.
Yang’s and Deb’s seminal work on the CSA illustrated an enormous computational cost savings when compared to the genetic algorithm and the particle swarm optimization Algorithm as each algorithm was used to find the solution to a handful of standard challenging mathematical functions: Michalewiczs, Rosenbrocks, Schwefels, Rastrigins, with a 96, 89, 96, and 91% decrease in the number of required fitness function evaluations when compared to a GA solution respectively [36].
\nWith a prevalence of evolutionary algorithms focused on solving trajectory generation, path planning, remote sensing, control theory, and parameter identification for aeronautical and astronautical applications it is a must to review the fundamentals of the most common evolutionary algorithms being used for those applications. Genetic algorithm, particle swarm optimization, firefly algorithm, ant colony optimization, artificial bee colony optimization, and the cuckoo search algorithm are presented and discussed with an emphasis on astronautical applications. In summary, the genetic algorithm and its variants can be used for a large parameter space but is more efficient in investigating a smaller parameter space, less than 1000 parameters in the chromosome. It is found that PID controller parameters, nonlinear parameter identification, and trajectory optimization are applications ripe for the genetic algorithm. Ant colony optimization, and artificial bee colony optimization are optimization routines more suited for combinatorics, such as with trajectory optimization, path planning, scheduling, and spacecraft load bearing. Particle swarm optimization, firefly algorithm, and cuckoo search algorithms are best suited for large parameter spaces due to the decrease in computation need and function calls when compared to the genetic algorithm family of optimizers. Key areas of investigation for these social evolution algorithms are in spacecraft trajectory planning, and in parameter identification.
\nEvolutionary algorithms have been shown to have a great potential to solve challenging problems that traditional optimization routines may not be able to tackle due to large computational need to support an exhaustive search of the solution space. The reader should now have the tools to take the foundational material presented here, to review the referenced sources, and conduct their own deep dive in an application area of interest.
\nThe views expressed in this paper are those of the author and do not reflect the official policy or position of the United States Air Force, Department of Defense, or U.S. Government.
\nPublic health protection is of paramount importance that demands the rapid and accurate detection and quantitation of microorganisms in potable water and in various raw and processed foods to prevent undesirable outbreaks of microbial contamination. Water quality has been assessed for potable and recreational activities using culture-dependent quantification and sensing of fecal indicator bacteria (FIB), such as total coliforms,
Waterborne diseases have been one of the major causes due to the consumption of contaminated water affecting seriously the public health of a humongous number of people in quick succession. In the 2014–2016 survey, the detection rate of pathogenic bacteria was 79.3%, followed by pathogenic
Between 2013 and 2016, a monocentric hospital-based investigation showed that
Coliforms, particularly
Traditional microbiological detection techniques consume time as
It is necessary to develop new approaches for detecting
PCR being a mighty and handy tool with molecular biologists showed enormous potential in various forms including multiplex PCR and quantitative real-time PCR. The advantage of PCR is that despite its inability to distinguish between live and dead cells, nonculturable cells may be detected rapidly. In the recent two decades, various PCR-based strategies have been introduced to improve the detection of indicator organisms [23, 24]. Genetic markers such as 23S rRNA and lacZ are often used to establish PCR tests for detecting
The
Li et al. [43] established a multiplex real-time PCR test that targets the
Being a rapid, sensitive, and specific method enabling the detection of multiple pathogens simultaneously this method finds applications in different types of foods and poultry industries. Nguyen et al. [45] developed a multiplex PCR for the rapid and simultaneous detection of three epidemic food-borne pathogens:
In developing countries, the identification of enteric pathogens in food and other edible items are time-consuming process and often results in wrong and delayed diagnosis. Enteropathogenic
Toma et al. [49] used a single-tube mPCR for the identification of enteropathogenic
Chen et al. [50] developed a multiplex rtPCR assay for the identification of diarrheagenic
Detection of harmful bacteria with higher specificity, sensitivity, and reliability is the focus of nucleic acid-based approaches. The desired nucleic acid sequence is hybridized to a synthetic oligonucleotide for specific detection of the pathogen [51]. Nucleic acid-based approaches are routinely used to detect bacterial infections and their toxin-producing genes [51]. Nucleic acid-based methods are rapid and easy to use, and they do not require the pathogens to be cultured (Figure 1).
Schematic depicting the steps in culture-independent detection of
Even a decade ago, the identification and measurement of specific target genes with absolute accuracy and as little as a few copies in a matter of hours was a dream. In the area of water quality assessment, however, qPCR technology has proven to be a powerful technique [53]. Unlike the classical PCR, which needs agarose-gel electrophoresis to identify the end-point PCR products, the qPCR enables assessing PCR product amplification by measuring fluorescence signals released by specialized dual-labeled probes or the intercalating dyes. The fluorescence intensity generated during the qPCR is directly related to the quantity of PCR products produced [12, 54, 55]. The most often used fluorescent systems for qPCR include SYBR green, TaqMan probes, and molecular beacons [56]. The qPCR techniques, which have higher specificity, sensitivity, and reliability than classic culture methods and mPCR [57], allow for the time-efficient detection of harmful bacteria with higher specificity, sensitivity, and reliability [12, 56, 58]. Although the qPCR has been used to detect and quantify
Utilizing TaqMan probes labeled with different fluorophores, microfluidic qPCR was shown to identify pathogens such as
In another study, Liu et al. [66] reported designing of the novel oligonucleotide primer set and TaqMan probes targeting the specific virulence genes of twelve common food pathogens such as
In order to circumvent the use of thermocyclers that entail the time-consuming thermal cycling, an innovative method such as isothermal DNA amplification has been introduced which finds its application in the advanced Research & Development (R & D) unit of the food industry. The LAMP reaction that involves isothermal amplification chemistry has a good range of possible applications, including point-of-care testing with the potential of getting developed into portable diagnostic systems, and quick testing of food products, clinical and environmental samples.
The isothermal characteristics of LAMP enable the simplification of the detection process without involving any costly and complex instrumentation wherein a simple heating block or a precise digital water bath would work. Though conventional PCR and LAMP techniques were reported to be vulnerable to several inhibitors while testing various biological (for example urinary and plant materials) matrices [64], yet LAMP is much less sensitive to amplification inhibitors [64], potentially permitting its application bypassing the general requirement for cultural enrichment or DNA purification.
Despite some disadvantages like its qualitative nature of detection, the LAMP offers several advantages over PCR. LAMP assay emphasizes the requirement of a heating block and obviates the need for a thermal cycler. Unlike PCR that requires DNA extraction from samples for amplification, LAMP assay does not require DNA extraction step. The difficulties in amplifying DNA in PCR from unprocessed urinary samples in the presence of a high concentration of urea were reported by Khan et al. [65]. Therefore the LAMP assay, by rendering the DNA extraction step redundant, has made the process more rapid and facile [67]. The implementation of LAMP does not require any denatured template as due to the use of Bst DNA polymerase from
Schematic representation of the principle steps in a LAMP assay and localization of the eight LAMP primers for specific amplification of target DNA. Adapted from Gallas-Lindemann et al. [
The very purpose of inner primers that consisted of two different sequences was to recognize a sense and antisense sequences of the target viral DNA, and the outer primers were designed to recognize an external sequence of the target viral DNA [69]. Additionally, in the LAMP assay, as an advantage, the identification of a positive reaction does not involve any special processing or electrophoresis. Only the visual observation of color change of the reaction mix in normal light is enabled when the appropriate DNA-binding dye is used. Thus, LAMP positive results could be better detected through visual observation of turbidity changes [70]. This visualization process can be improved by a UV transilluminator. Hill et al. [67] had demonstrated the use of propidium iodide for detecting the LAMP products.
In order to detect generic
It is to be noted that the LAMP assay reported by Hill et al. [67] was able to detect a large number of strains with very high sensitivity. Since biological samples such as cerebrospinal fluid and blood require very high sensitivity as compared to urine samples LAMP can be suitably modified for its clinical uses. LAMP has also been proposed to detect a lower copy number in partially treated infections (post-empirical antibiotic doses) [67].
A biosensor typically consists of a bioreceptor element with a transducer. The bioreceptor, interacts specifically with the analyte, whereas the transducer converts the biomolecular interaction into an electronic signal. Three basic parts of a biosensor are recognition material, transducer or detector system, and signal processor [74]. Monitoring the molecular interaction between the DNA-based bioreceptor and the analyte is an essential element of various DNA-based sensing strategies. The measurement methods of DNA–DNA interactions that take place on the various sensor surfaces are gaining much interest to improve sensor performance. The assays are applicable to the determination of low numbers of
Arora et al. [77] reported an electrochemical DNA biosensor for the detection of
Since its discovery in the 1980s, the system has demonstrated widespread applications in basic biotechnology research and disease treatment [80, 81]. A pressing need of the hour is the availability of a cost-efficient, rapid and selective molecular diagnostic platform to detect different pathogens and lethal diseases in the early stage of the infection. Quantitative PCR and metagenomic next-generation sequencing (mNGS) are the most commonly explored molecular platforms for the same; however, these methods have their disadvantages and limitations. Clustered Regularly Interspaced Short Palindromic Repeat/associated protein (CRISPR/Cas)-based diagnostic platform for the detection of nucleic acids has progressively demonstrated its potential as an ideal diagnostic approach for pathogens, cancer biomarker, and single-nucleotide polymorphisms (SNPs) detection. CRISPR systems have evolved in prokaryotes as a defensive mechanism against foreign viruses by cleaving their nucleic acids [82, 83, 84].
Additionally, the unique cleavage activity of Cas9 is often utilized for the development of ultra-low abundance DNA biosensors. A highly innovative and sensitive CRISPR/Cas9 system was developed by Huang et al. [84] that triggered isothermal exponential amplification reaction (CAS-EXPAR) strategy to detect DNA targets with attomolar (aM) sensitivity and single-base specificity [84]. CAS-EXPAR was primed by the target DNA fragment produced by cleavage of CRISPR/Cas9, and associated with the cyclical amplification reaction to produce numerous DNA replicates capable of getting detected by a real-time SYBR Green fluorescence signal [83].
Recently, Sun et al. [84] reported the detection of
CRISPR/Cas9 platform coupled with two-step isothermal amplification for detection of
Molecular diagnostic platforms have become promising alternatives to traditional methods for
The authors declare no conflict of interest.
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We first introduce a general framework and several conventional models for functional data, including the functional linear model, penalized regression splines, and the spatial temporal model. However, in engineering practice, a naive mathematical modeling of functional response may fail due to the lack of expressing the underlying physical mechanism. We propose a series of quasiphysical models to handle the functional response. A motivating example of metamaterial design is thoroughly discussed to demonstrate the idea of quasiphysical models. In real applications, various uncertainties have to be taken into account, such as that of the permittivity or permeability of the substrate of the metamaterial. For the propagation of uncertainty, simulation‐based methods are discussed. A Bayesian framework is presented to deal with the model calibration in the case of functional response. Experimental results illustrate the efficiency of the proposed method.",book:{id:"5832",slug:"uncertainty-quantification-and-model-calibration",title:"Uncertainty Quantification and Model Calibration",fullTitle:"Uncertainty Quantification and Model Calibration"},signatures:"Xiao Guo, Yang He, Binbin Zhu, Yang Yang, Ke Deng, Ruopeng Liu\nand Chunlin Ji",authors:[{id:"198216",title:"Dr.",name:"Chunlin",middleName:null,surname:"Ji",slug:"chunlin-ji",fullName:"Chunlin Ji"},{id:"199075",title:"Dr.",name:"Xiao",middleName:null,surname:"Guo",slug:"xiao-guo",fullName:"Xiao Guo"}]}],mostDownloadedChaptersLast30Days:[{id:"54982",title:"Polynomial Chaos Expansion for Probabilistic Uncertainty Propagation",slug:"polynomial-chaos-expansion-for-probabilistic-uncertainty-propagation",totalDownloads:2869,totalCrossrefCites:6,totalDimensionsCites:11,abstract:"Uncertainty propagation (UP) methods are of great importance to design optimization under uncertainty. As a well-known and rigorous probabilistic UP approach, the polynomial chaos expansion (PCE) technique has been widely studied and applied. However, there is a lack of comprehensive overviews and studies of the latest advances of the PCE methods, and there is still a large gap between the academic research and engineering application for PCE due to its high computational cost. In this chapter, latest advances of the PCE theory and method are elaborated, in which the newly developed data-driven PCE method that does not depend on the complete information of input probabilistic distribution as the common PCE approaches is introduced and improved. Meanwhile, the least angle regression technique and the trust region scenario are, respectively, extended to reduce the computational cost of data-driven PCE to accommodate it to practical engineering design applications. In addition, comprehensive comparisons are made to explore the relative merits of the most commonly used PCE approaches in the literature to help designers to choose more suitable PCE techniques in probabilistic design optimization.",book:{id:"5832",slug:"uncertainty-quantification-and-model-calibration",title:"Uncertainty Quantification and Model Calibration",fullTitle:"Uncertainty Quantification and Model Calibration"},signatures:"Shuxing Yang, Fenfen Xiong and Fenggang Wang",authors:[{id:"200594",title:"Dr.",name:"Fenfen",middleName:null,surname:"Xiong",slug:"fenfen-xiong",fullName:"Fenfen Xiong"},{id:"200601",title:"Prof.",name:"Shuxing",middleName:null,surname:"Yang",slug:"shuxing-yang",fullName:"Shuxing Yang"},{id:"205382",title:"Mr.",name:"Fenggang",middleName:null,surname:"Wang",slug:"fenggang-wang",fullName:"Fenggang Wang"}]},{id:"54755",title:"Fitting Models to Data: Residual Analysis, a Primer",slug:"fitting-models-to-data-residual-analysis-a-primer",totalDownloads:2503,totalCrossrefCites:10,totalDimensionsCites:16,abstract:"The aim of this chapter is to show checking the underlying assumptions (the errors are independent, have a zero mean, a constant variance and follows a normal distribution) in a regression analysis, mainly fitting a straight‐line model to experimental data, via the residual plots. Residuals play an essential role in regression diagnostics; no analysis is being complete without a thorough examination of residuals. The residuals should show a trend that tends to confirm the assumptions made in performing the regression analysis, or failing them should not show a tendency that denies them. Although there are numerical statistical means of verifying observed discrepancies, statisticians often prefer a visual examination of residual graphs as a more informative and certainly more convenient methodology. When dealing with small samples, the use of the graphic techniques can be very useful. Several examples taken from scientific journals and monographs are selected dealing with linearity, calibration, heteroscedastic data, errors in the model, transforming data, time‐order analysis and non‐linear calibration curves.",book:{id:"5832",slug:"uncertainty-quantification-and-model-calibration",title:"Uncertainty Quantification and Model Calibration",fullTitle:"Uncertainty Quantification and Model Calibration"},signatures:"Julia Martin, David Daffos Ruiz de Adana and Agustin G. Asuero",authors:[{id:"190870",title:"Dr.",name:"Agustín G.",middleName:null,surname:"Asuero",slug:"agustin-g.-asuero",fullName:"Agustín G. Asuero"},{id:"190871",title:"Dr.",name:"Julia",middleName:null,surname:"Martín",slug:"julia-martin",fullName:"Julia Martín"},{id:"203694",title:"Mr.",name:"David",middleName:null,surname:"Daffos Ruiz De Adana",slug:"david-daffos-ruiz-de-adana",fullName:"David Daffos Ruiz De Adana"},{id:"203695",title:"Mr.",name:"Alberto",middleName:null,surname:"Romero Gracia",slug:"alberto-romero-gracia",fullName:"Alberto Romero Gracia"}]},{id:"55003",title:"Uncertainty Quantification and Reduction of Molecular Dynamics Models",slug:"uncertainty-quantification-and-reduction-of-molecular-dynamics-models",totalDownloads:1439,totalCrossrefCites:2,totalDimensionsCites:5,abstract:"Molecular dynamics (MD) is an important method underlying the modern field of Computational Materials Science. Without requiring prior knowledge as inputs, MD simulations have been used to study a variety of material problems. However, results of molecular dynamics simulations are often associated with errors as compared with experimental observations. These errors come from a variety of sources, including inaccuracy of interatomic potentials, short length and time scales, idealized problem description and statistical uncertainties of MD simulations themselves. This chapter specifically devotes to the statistical uncertainties of MD simulations. In particular, methods to quantify and reduce such statistical uncertainties are demonstrated using a variety of exemplar cases, including calculations of finite temperature static properties such as lattice constants, cohesive energies, elastic constants, dislocation energies, thermal conductivities, surface segregation and calculations of kinetic properties such as diffusion parameters. We also demonstrate that when the statistical uncertainties are reduced to near zero, MD can be used to validate and improve widely used theories.",book:{id:"5832",slug:"uncertainty-quantification-and-model-calibration",title:"Uncertainty Quantification and Model Calibration",fullTitle:"Uncertainty Quantification and Model Calibration"},signatures:"Xiaowang Zhou and Stephen M. Foiles",authors:[{id:"201277",title:"Dr.",name:"Xiaowang",middleName:null,surname:"Zhou",slug:"xiaowang-zhou",fullName:"Xiaowang Zhou"},{id:"205437",title:"Dr.",name:"Stephen M.",middleName:null,surname:"Foiles",slug:"stephen-m.-foiles",fullName:"Stephen M. Foiles"}]},{id:"54841",title:"State‐of‐the‐Art Nonprobabilistic Finite Element Analyses",slug:"state-of-the-art-nonprobabilistic-finite-element-analyses",totalDownloads:1647,totalCrossrefCites:0,totalDimensionsCites:0,abstract:"The finite element analysis of a mechanical system is conventionally performed in the context of deterministic inputs. However, uncertainties associated with material properties, geometric dimensions, subjective experiences, boundary conditions, and external loads are ubiquitous in engineering applications. The most popular techniques to handle these uncertain parameters are the probabilistic methods, in which uncertainties are modeled as random variables or stochastic processes based on a large amount of statistical information on each uncertain parameter. Nevertheless, subjective results could be obtained if insufficient information unavailable and nonprobabilistic methods can be alternatively employed, which has led to elegant procedures for the nonprobabilistic finite element analysis. In this chapter, each nonprobabilistic finite element analysis method can be decomposed as two individual parts, i.e., the core algorithm and preprocessing procedure. In this context, four types of algorithms and two typical preprocessing procedures as well as their effectiveness were described in detail, based on which novel hybrid algorithms can be conceived for the specific problems and the future work in this research field can be fostered.",book:{id:"5832",slug:"uncertainty-quantification-and-model-calibration",title:"Uncertainty Quantification and Model Calibration",fullTitle:"Uncertainty Quantification and Model Calibration"},signatures:"Wang Lei, Qiu Zhiping and Zheng Yuning",authors:[{id:"196882",title:"Prof.",name:"Zhiping",middleName:null,surname:"Qiu",slug:"zhiping-qiu",fullName:"Zhiping Qiu"},{id:"198421",title:"Dr.",name:"Lei",middleName:null,surname:"Wang",slug:"lei-wang",fullName:"Lei Wang"},{id:"204754",title:"Dr.",name:"Yuning",middleName:null,surname:"Zheng",slug:"yuning-zheng",fullName:"Yuning Zheng"}]},{id:"55556",title:"An Improved Wavelet‐Based Multivariable Fault Detection Scheme",slug:"an-improved-wavelet-based-multivariable-fault-detection-scheme",totalDownloads:1542,totalCrossrefCites:3,totalDimensionsCites:3,abstract:"Data observed from environmental and engineering processes are usually noisy and correlated in time, which makes the fault detection more difficult as the presence of noise degrades fault detection quality. Multiscale representation of data using wavelets is a powerful feature extraction tool that is well suited to denoising and decorrelating time series data. In this chapter, we combine the advantages of multiscale partial least squares (MSPLSs) modeling with those of the univariate EWMA (exponentially weighted moving average) monitoring chart, which results in an improved fault detection system, especially for detecting small faults in highly correlated, multivariate data. Toward this end, we applied EWMA chart to the output residuals obtained from MSPLS model. It is shown through simulated distillation column data the significant improvement in fault detection can be obtained by using the proposed methods as compared to the use of the conventional partial least square (PLS)‐based Q and EWMA methods and MSPLS‐based Q method.",book:{id:"5832",slug:"uncertainty-quantification-and-model-calibration",title:"Uncertainty Quantification and Model Calibration",fullTitle:"Uncertainty Quantification and Model Calibration"},signatures:"Fouzi Harrou, Ying Sun and Muddu Madakyaru",authors:[{id:"197090",title:"Dr.",name:"Fouzi",middleName:null,surname:"Harrou",slug:"fouzi-harrou",fullName:"Fouzi Harrou"}]}],onlineFirstChaptersFilter:{topicId:"613",limit:6,offset:0},onlineFirstChaptersCollection:[{id:"82166",title:"Evaluation of Principal Component Analysis Variants to Assess Their Suitability for Mobile Malware Detection",slug:"evaluation-of-principal-component-analysis-variants-to-assess-their-suitability-for-mobile-malware-d",totalDownloads:10,totalDimensionsCites:0,doi:"10.5772/intechopen.105418",abstract:"Principal component analysis (PCA) is an unsupervised machine learning algorithm that plays a vital role in reducing the dimensions of the data in building an appropriate machine learning model. It is a statistical process that transforms the data containing correlated features into a set of uncorrelated features with the help of orthogonal transformations. Unsupervised machine learning is a concept of self-learning method that involves unlabelled data to identify hidden patterns. PCA converts the data features from a high dimensional space into a low dimensional space. PCA also acts as a feature extraction method since it transforms the ‘n’ number of features into ‘m’ number of principal components (PCs; m < n). Mobile Malware is increasing tremendously in the digital era due to the growth of android mobile users and android applications. Some of the mobile malware are viruses, Trojan horses, worms, adware, spyware, ransomware, riskware, banking malware, SMS malware, keylogger, and many more. To automate the process of detecting mobile malware without human intervention, machine learning methods are applied to discover the malware more precisely. Specifically, unsupervised machine learning helps to uncover the hidden patterns to detect anomalies in the data. In discovering hidden patterns of malware, PCA is an important dimensionality reduction technique that can be applied to transform the features into PCs containing important feature values. So, by implementing PCA, the correlated features are transformed into uncorrelated features automatically to explore the anomalies in the data effectively. This book chapter explains all the variants of the PCA, including all linear and non-linear methods of PCA and their suitability in applying to mobile malware detection. A case study on mobile malware detection with variants of PCA using machine learning techniques in CICMalDroid_2020 dataset has been experimented. Based on the experimental results, for the given dataset, normal PCA is suitable to detect the malware data points and forms an optimal cluster.",book:{id:"11201",title:"Advances in Principal Component Analysis",coverURL:"https://cdn.intechopen.com/books/images_new/11201.jpg"},signatures:"Padmavathi Ganapathi, Shanmugapriya Dhathathri and Roshni Arumugam"},{id:"81645",title:"Determining an Adequate Number of Principal Components",slug:"determining-an-adequate-number-of-principal-components",totalDownloads:11,totalDimensionsCites:0,doi:"10.5772/intechopen.104534",abstract:"The problem of choosing the number of PCs to retain is analyzed in the context of model selection, using so-called model selection criteria (MSCs). For a prespecified set of models, indexed by k=1,2,…,K, these model selection criteria (MSCs) take the form MSCk=nLLk+anmk, where, for model k,LLk is the maximum log likelihood, mk is the number of independent parameters, and the constant an is an=lnn for BIC and an=2 for AIC. The maximum log likelihood LLk is achieved by using the maximum likelihood estimates (MLEs) of the parameters. In Gaussian models, LLk involves the logarithm of the mean squared error (MSE). The main contribution of this chapter is to show how to best use BIC to choose the number of PCs, and to compare these results to ad hoc procedures that have been used. Findings include the following. These are stated as they apply to the eigenvalues of the correlation matrix, which are between 0 and p and have an average of 1. For considering an additional PCk + 1, with AIC, inclusion of the additional PCk + 1 is justified if the corresponding eigenvalue λk+1 is greater than exp−2/n. For BIC, the inclusion of an additional PCk + 1 is justified if λk+1>n1/n, which tends to 1 for large n. Therefore, this is in approximate agreement with the average eigenvalue rule for correlation matrices, stating that one should retain dimensions with eigenvalues larger than 1.",book:{id:"11201",title:"Advances in Principal Component Analysis",coverURL:"https://cdn.intechopen.com/books/images_new/11201.jpg"},signatures:"Stanley L. Sclove"},{id:"81542",title:"On the Use of Modified Winsorization with Graphical Diagnostic for Obtaining a Statistically Optimal Classification Accuracy in Predictive Discriminant Analysis",slug:"on-the-use-of-modified-winsorization-with-graphical-diagnostic-for-obtaining-a-statistically-optimal",totalDownloads:14,totalDimensionsCites:0,doi:"10.5772/intechopen.104539",abstract:"In predictive discriminant analysis (PDA), the classification accuracy is only statistically optimal if each group sample is normally distributed with different group means, and each predictor variance is similar between the groups. This can be achieved by accounting for homogeneity of variances between the groups using the modified winsorization with graphical diagnostic (MW-GD) method. The MW-GD method involves the identification and removal of legitimate contaminants in a training sample with the aim of obtaining a true optimal training sample that can be used to build a predictive discriminant function (PDF) that will yield a statistically optimal classification accuracy. However, the use of this method is yet to receive significant attention in PDA. An alternative statistical interpretation of the graphical diagnostic information associated with the method when confronted with the challenge of differentiating between a variable shape in the groups of the 2-D area plot remains a problem to be resolved. Therefore, this paper provides a more comprehensive analysis of the idea and concept of the MW-GD method, as well as proposed an alternative statistical interpretation of the informative graphical diagnostic associated with the method when confronted with the challenge of differentiating between a variable shape in the groups of the 2-D area plot.",book:{id:"11201",title:"Advances in Principal Component Analysis",coverURL:"https://cdn.intechopen.com/books/images_new/11201.jpg"},signatures:"Augustine Iduseri"},{id:"81460",title:"Spatial Principal Component Analysis of Head-Related Transfer Functions and Its Domain Dependency",slug:"spatial-principal-component-analysis-of-head-related-transfer-functions-and-its-domain-dependency",totalDownloads:15,totalDimensionsCites:0,doi:"10.5772/intechopen.104449",abstract:"In this chapter, the Principal Component Analysis (PCA) was adopted to spatial variation of Head-Related Transfer Function (HRTF) or its corresponding inverse Fourier Transform, called Head-Related Impulse Response (HRIR), in order to compactly represent their spatial variation. This is called the Spatial PCA (SPCA). The SPCA was carried out for a database of HRTFs in all directions by selecting the domain as one of the HRIRs, the complex HRTFs, the frequency amplitudes of HRTFs, log-amplitudes of HRTFs, and complex logarithm of HRTFs. The minimum phase approximation was incorporated for the frequency amplitudes and log-amplitudes of HRTFs. Comparison of the accuracies in both time and frequency domains taking into account their influence on subjective evaluation showed that the log-amplitudes and complex logarithm of HRTFs are suitable for the SPCA of HRTFs.",book:{id:"11201",title:"Advances in Principal Component Analysis",coverURL:"https://cdn.intechopen.com/books/images_new/11201.jpg"},signatures:"Shouichi Takane"},{id:"81407",title:"Space-Time-Parameter PCA for Data-Driven Modeling with Application to Bioengineering",slug:"space-time-parameter-pca-for-data-driven-modeling-with-application-to-bioengineering",totalDownloads:28,totalDimensionsCites:0,doi:"10.5772/intechopen.103756",abstract:"Principal component analysis is a recognized powerful and practical method in statistics and data science. It can also be used in modeling as a dimensionality reduction tool to achieve low-order models of complex multiphysics or engineering systems. Model-order reduction (MOR) methodologies today are an important topic for engineering design and analysis. Design space exploration or accelerated numerical optimization for example are made easier by the use of reduced-order models. In this chapter, we will talk about the use of higher-order singular value decompositions (HOSVD) applied to spatiotemporal problems that are parameterized by a set of design variables or physical parameters. Here we consider a data-driven reduced order modeling based on a design of computer experiment: from high-dimensional computational results returned by high-fidelity solvers (e.g. finite element ones), the HOSVD allows us to determine spatial, time and parameters principal components. The dynamics of the system can then be retrieved by identifying the low-order discrete dynamical system. As application, we will consider the dynamics of deformable capsules flowing into microchannels. The study of such fluid-structure interaction problems is motivated by the use of microcapsules as innovative drug delivery carriers through blood vessels.",book:{id:"11201",title:"Advances in Principal Component Analysis",coverURL:"https://cdn.intechopen.com/books/images_new/11201.jpg"},signatures:"Florian De Vuyst, Claire Dupont and Anne-Virginie Salsac"},{id:"81414",title:"Prediction Analysis Based on Logistic Regression Modelling",slug:"prediction-analysis-based-on-logistic-regression-modelling",totalDownloads:14,totalDimensionsCites:0,doi:"10.5772/intechopen.103090",abstract:"The chapter aims to show an application of logistic regression modelling for prediction analysis in the offshore industry. The different variables shown in dynamic positioning incident reports are analysed and processed using logistic regression modelling. The results of the models are then analysed, showing which data influence the loss of positioning and human errors and how the model can be interpreted. Afterwards, and based on the obtained models, operational limits can be proposed to reduce downtimes and thus improve the safety of the operations and the productivity of the offshore operations when using dynamic positioning systems.",book:{id:"11201",title:"Advances in Principal Component Analysis",coverURL:"https://cdn.intechopen.com/books/images_new/11201.jpg"},signatures:"Zaloa Sanchez-Varela"}],onlineFirstChaptersTotal:12},preDownload:{success:null,errors:{}},subscriptionForm:{success:null,errors:{}},aboutIntechopen:{},privacyPolicy:{},peerReviewing:{},howOpenAccessPublishingWithIntechopenWorks:{},sponsorshipBooks:{sponsorshipBooks:[],offset:8,limit:8,total:0},allSeries:{pteSeriesList:[{id:"14",title:"Artificial Intelligence",numberOfPublishedBooks:11,numberOfPublishedChapters:91,numberOfOpenTopics:6,numberOfUpcomingTopics:0,issn:"2633-1403",doi:"10.5772/intechopen.79920",isOpenForSubmission:!0},{id:"7",title:"Biomedical Engineering",numberOfPublishedBooks:12,numberOfPublishedChapters:108,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2631-5343",doi:"10.5772/intechopen.71985",isOpenForSubmission:!0}],lsSeriesList:[{id:"11",title:"Biochemistry",numberOfPublishedBooks:33,numberOfPublishedChapters:332,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2632-0983",doi:"10.5772/intechopen.72877",isOpenForSubmission:!0},{id:"25",title:"Environmental Sciences",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2754-6713",doi:"10.5772/intechopen.100362",isOpenForSubmission:!0},{id:"10",title:"Physiology",numberOfPublishedBooks:14,numberOfPublishedChapters:145,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-8261",doi:"10.5772/intechopen.72796",isOpenForSubmission:!0}],hsSeriesList:[{id:"3",title:"Dentistry",numberOfPublishedBooks:11,numberOfPublishedChapters:142,numberOfOpenTopics:2,numberOfUpcomingTopics:0,issn:"2631-6218",doi:"10.5772/intechopen.71199",isOpenForSubmission:!0},{id:"6",title:"Infectious Diseases",numberOfPublishedBooks:13,numberOfPublishedChapters:124,numberOfOpenTopics:4,numberOfUpcomingTopics:0,issn:"2631-6188",doi:"10.5772/intechopen.71852",isOpenForSubmission:!0},{id:"13",title:"Veterinary Medicine and Science",numberOfPublishedBooks:11,numberOfPublishedChapters:112,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2632-0517",doi:"10.5772/intechopen.73681",isOpenForSubmission:!0}],sshSeriesList:[{id:"22",title:"Business, Management and Economics",numberOfPublishedBooks:1,numberOfPublishedChapters:22,numberOfOpenTopics:3,numberOfUpcomingTopics:0,issn:"2753-894X",doi:"10.5772/intechopen.100359",isOpenForSubmission:!0},{id:"23",title:"Education and Human Development",numberOfPublishedBooks:0,numberOfPublishedChapters:12,numberOfOpenTopics:1,numberOfUpcomingTopics:1,issn:null,doi:"10.5772/intechopen.100360",isOpenForSubmission:!0},{id:"24",title:"Sustainable Development",numberOfPublishedBooks:1,numberOfPublishedChapters:19,numberOfOpenTopics:5,numberOfUpcomingTopics:0,issn:"2753-6580",doi:"10.5772/intechopen.100361",isOpenForSubmission:!0}],testimonialsList:[{id:"6",text:"It is great to work with the IntechOpen to produce a worthwhile collection of research that also becomes a great educational resource and guide for future research endeavors.",author:{id:"259298",name:"Edward",surname:"Narayan",institutionString:null,profilePictureURL:"https://mts.intechopen.com/storage/users/259298/images/system/259298.jpeg",slug:"edward-narayan",institution:{id:"3",name:"University of Queensland",country:{id:null,name:"Australia"}}}},{id:"13",text:"The collaboration with and support of the technical staff of IntechOpen is fantastic. 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",coverUrl:"https://cdn.intechopen.com/series/covers/23.jpg",latestPublicationDate:"August 17th, 2022",hasOnlineFirst:!0,numberOfPublishedBooks:0,editor:{id:"280770",title:"Dr.",name:"Katherine K.M.",middleName:null,surname:"Stavropoulos",slug:"katherine-k.m.-stavropoulos",fullName:"Katherine K.M. Stavropoulos",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRdFuQAK/Profile_Picture_2022-05-24T09:03:48.jpg",biography:"Katherine Stavropoulos received her BA in Psychology from Trinity College, in Connecticut, USA and her Ph.D. in Experimental Psychology from the University of California, San Diego. She completed her postdoctoral work at the Yale Child Study Center with Dr. James McPartland. Dr. Stavropoulos’ doctoral dissertation explored neural correlates of reward anticipation to social versus nonsocial stimuli in children with and without autism spectrum disorders (ASD). She has been a faculty member at the University of California, Riverside in the School of Education since 2016. Her research focuses on translational studies to explore the reward system in ASD, as well as how anxiety contributes to social challenges in ASD. She also investigates how behavioral interventions affect neural activity, behavior, and school performance in children with ASD. She is also involved in the diagnosis of children with ASD and is a licensed clinical psychologist in California. She is the Assistant Director of the SEARCH Center at UCR and is a faculty member in the Graduate Program in Neuroscience.",institutionString:null,institution:{name:"University of California, Riverside",institutionURL:null,country:{name:"United States of America"}}},editorTwo:null,editorThree:null},subseries:{paginationCount:2,paginationItems:[{id:"89",title:"Education",coverUrl:"https://cdn.intechopen.com/series_topics/covers/89.jpg",isOpenForSubmission:!1,editor:{id:"260066",title:"Associate Prof.",name:"Michail",middleName:null,surname:"Kalogiannakis",slug:"michail-kalogiannakis",fullName:"Michail Kalogiannakis",profilePictureURL:"https://mts.intechopen.com/storage/users/260066/images/system/260066.jpg",biography:"Michail Kalogiannakis is an Associate Professor of the Department of Preschool Education, University of Crete, and an Associate Tutor at School of Humanities at the Hellenic Open University. He graduated from the Physics Department of the University of Crete and continued his post-graduate studies at the University Paris 7-Denis Diderot (D.E.A. in Didactic of Physics), University Paris 5-René Descartes-Sorbonne (D.E.A. in Science Education) and received his Ph.D. degree at the University Paris 5-René Descartes-Sorbonne (PhD in Science Education). His research interests include science education in early childhood, science teaching and learning, e-learning, the use of ICT in science education, games simulations, and mobile learning. He has published over 120 articles in international conferences and journals and has served on the program committees of numerous international conferences.",institutionString:"University of Crete",institution:{name:"University of Crete",institutionURL:null,country:{name:"Greece"}}},editorTwo:{id:"422488",title:"Dr.",name:"Maria",middleName:null,surname:"Ampartzaki",slug:"maria-ampartzaki",fullName:"Maria Ampartzaki",profilePictureURL:"https://mts.intechopen.com/storage/users/422488/images/system/422488.jpg",biography:"Dr Maria Ampartzaki is an Assistant Professor in Early Childhood Education in the Department of Preschool Education at the University of Crete. Her research interests include ICT in education, science education in the early years, inquiry-based and art-based learning, teachers’ professional development, action research, and the Pedagogy of Multiliteracies, among others. 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His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"94311",title:"Prof.",name:"Martins",middleName:"Ochubiojo",surname:"Ochubiojo Emeje",slug:"martins-ochubiojo-emeje",fullName:"Martins Ochubiojo Emeje",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/94311/images/system/94311.jpeg",biography:"Martins Emeje obtained a BPharm with distinction from Ahmadu Bello University, Nigeria, and an MPharm and Ph.D. from the University of Nigeria (UNN), where he received the best Ph.D. award and was enlisted as UNN’s “Face of Research.” He established the first nanomedicine center in Nigeria and was the pioneer head of the intellectual property and technology transfer as well as the technology innovation and support center. Prof. Emeje’s several international fellowships include the prestigious Raman fellowship. He has published more than 150 articles and patents. He is also the head of R&D at NIPRD and holds a visiting professor position at Nnamdi Azikiwe University, Nigeria. He has a postgraduate certificate in Project Management from Walden University, Minnesota, as well as a professional teaching certificate and a World Bank certification in Public Procurement. Prof. Emeje was a national chairman of academic pharmacists in Nigeria and the 2021 winner of the May & Baker Nigeria Plc–sponsored prize for professional service in research and innovation.",institutionString:"National Institute for Pharmaceutical Research and Development",institution:{name:"National Institute for Pharmaceutical Research and Development",country:{name:"Nigeria"}}},{id:"436430",title:"Associate Prof.",name:"Mesut",middleName:null,surname:"Işık",slug:"mesut-isik",fullName:"Mesut Işık",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/436430/images/19686_n.jpg",biography:null,institutionString:null,institution:{name:"Bilecik University",country:{name:"Turkey"}}},{id:"268659",title:"Ms.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/268659/images/8143_n.jpg",biography:"Dr. Zhan received his undergraduate and graduate training in the fields of preventive medicine and epidemiology and statistics at the West China University of Medical Sciences in China during 1989 to 1999. He received his post-doctoral training in oncology and cancer proteomics for two years at the Cancer Research Institute of Human Medical University in China. In 2001, he went to the University of Tennessee Health Science Center (UTHSC) in USA, where he was a post-doctoral researcher and focused on mass spectrometry and cancer proteomics. Then, he was appointed as an Assistant Professor of Neurology, UTHSC in 2005. He moved to the Cleveland Clinic in USA as a Project Scientist/Staff in 2006 where he focused on the studies of eye disease proteomics and biomarkers. He returned to UTHSC as an Assistant Professor of Neurology in the end of 2007, engaging in proteomics and biomarker studies of lung diseases and brain tumors, and initiating the studies of predictive, preventive, and personalized medicine (PPPM) in cancer. In 2010, he was promoted to Associate Professor of Neurology, UTHSC. Currently, he is a Professor at Xiangya Hospital of Central South University in China, Fellow of Royal Society of Medicine (FRSM), the European EPMA National Representative in China, Regular Member of American Association for the Advancement of Science (AAAS), European Cooperation of Science and Technology (e-COST) grant evaluator, Associate Editors of BMC Genomics, BMC Medical Genomics, EPMA Journal, and Frontiers in Endocrinology, Executive Editor-in-Chief of Med One. He has\npublished 116 peer-reviewed research articles, 16 book chapters, 2 books, and 2 US patents. His current main research interest focuses on the studies of cancer proteomics and biomarkers, and the use of modern omics techniques and systems biology for PPPM in cancer, and on the development and use of 2DE-LC/MS for the large-scale study of human proteoforms.",institutionString:null,institution:{name:"Xiangya Hospital Central South University",country:{name:"China"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"418340",title:"Dr.",name:"Jyotirmoi",middleName:null,surname:"Aich",slug:"jyotirmoi-aich",fullName:"Jyotirmoi Aich",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038Ugi5QAC/Profile_Picture_2022-04-15T07:48:28.png",biography:"Biotechnologist with 15 years of research including 6 years of teaching experience. Demonstrated record of scientific achievements through consistent publication record (H index = 13, with 874 citations) in high impact journals such as Nature Communications, Oncotarget, Annals of Oncology, PNAS, and AJRCCM, etc. Strong research professional with a post-doctorate from ACTREC where I gained experimental oncology experience in clinical settings and a doctorate from IGIB where I gained expertise in asthma pathophysiology. A well-trained biotechnologist with diverse experience on the bench across different research themes ranging from asthma to cancer and other infectious diseases. An individual with a strong commitment and innovative mindset. Have the ability to work on diverse projects such as regenerative and molecular medicine with an overall mindset of improving healthcare.",institutionString:"DY Patil Deemed to Be University",institution:null},{id:"349288",title:"Prof.",name:"Soumya",middleName:null,surname:"Basu",slug:"soumya-basu",fullName:"Soumya Basu",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000035QxIDQA0/Profile_Picture_2022-04-15T07:47:01.jpg",biography:"Soumya Basu, Ph.D., is currently working as an Associate Professor at Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India. With 16+ years of trans-disciplinary research experience in Drug Design, development, and pre-clinical validation; 20+ research article publications in journals of repute, 9+ years of teaching experience, trained with cross-disciplinary education, Dr. Basu is a life-long learner and always thrives for new challenges.\r\nHer research area is the design and synthesis of small molecule partial agonists of PPAR-γ in lung cancer. She is also using artificial intelligence and deep learning methods to understand the exosomal miRNA’s role in cancer metastasis. Dr. Basu is the recipient of many awards including the Early Career Research Award from the Department of Science and Technology, Govt. of India. She is a reviewer of many journals like Molecular Biology Reports, Frontiers in Oncology, RSC Advances, PLOS ONE, Journal of Biomolecular Structure & Dynamics, Journal of Molecular Graphics and Modelling, etc. She has edited and authored/co-authored 21 journal papers, 3 book chapters, and 15 abstracts. She is a Board of Studies member at her university. She is a life member of 'The Cytometry Society”-in India and 'All India Cell Biology Society”- in India.",institutionString:"Dr. D.Y. Patil Vidyapeeth, Pune",institution:{name:"Dr. D.Y. Patil Vidyapeeth, Pune",country:{name:"India"}}},{id:"354817",title:"Dr.",name:"Anubhab",middleName:null,surname:"Mukherjee",slug:"anubhab-mukherjee",fullName:"Anubhab Mukherjee",position:null,profilePictureURL:"https://intech-files.s3.amazonaws.com/0033Y0000365PbRQAU/ProfilePicture%202022-04-15%2005%3A11%3A18.480",biography:"A former member of Laboratory of Nanomedicine, Brigham and Women’s Hospital, Harvard University, Boston, USA, Dr. Anubhab Mukherjee is an ardent votary of science who strives to make an impact in the lives of those afflicted with cancer and other chronic/acute ailments. He completed his Ph.D. from CSIR-Indian Institute of Chemical Technology, Hyderabad, India, having been skilled with RNAi, liposomal drug delivery, preclinical cell and animal studies. He pursued post-doctoral research at College of Pharmacy, Health Science Center, Texas A & M University and was involved in another postdoctoral research at Department of Translational Neurosciences and Neurotherapeutics, John Wayne Cancer Institute, Santa Monica, California. In 2015, he worked in Harvard-MIT Health Sciences & Technology as a visiting scientist. He has substantial experience in nanotechnology-based formulation development and successfully served various Indian organizations to develop pharmaceuticals and nutraceutical products. He is an inventor in many US patents and an author in many peer-reviewed articles, book chapters and books published in various media of international repute. Dr. Mukherjee is currently serving as Principal Scientist, R&D at Esperer Onco Nutrition (EON) Pvt. Ltd. and heads the Hyderabad R&D center of the organization.",institutionString:"Esperer Onco Nutrition Pvt Ltd.",institution:null},{id:"319365",title:"Assistant Prof.",name:"Manash K.",middleName:null,surname:"Paul",slug:"manash-k.-paul",fullName:"Manash K. Paul",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/319365/images/system/319365.png",biography:"Manash K. Paul is a scientist and Principal Investigator at the University of California Los Angeles. He has contributed significantly to the fields of stem cell biology, regenerative medicine, and lung cancer. His research focuses on various signaling processes involved in maintaining stem cell homeostasis during the injury-repair process, deciphering the lung stem cell niche, pulmonary disease modeling, immuno-oncology, and drug discovery. He is currently investigating the role of extracellular vesicles in premalignant lung cell migration and detecting the metastatic phenotype of lung cancer via artificial intelligence-based analyses of exosomal Raman signatures. Dr. Paul also works on spatial multiplex immunofluorescence-based tissue mapping to understand the immune repertoire in lung cancer. Dr. Paul has published in more than sixty-five peer-reviewed international journals and is highly cited. He is the recipient of many awards, including the UCLA Vice Chancellor’s award and the 2022 AAISCR-R Vijayalaxmi Award for Innovative Cancer Research. He is a senior member of the Institute of Electrical and Electronics Engineers (IEEE) and an editorial board member for several international journals.",institutionString:"University of California Los Angeles",institution:{name:"University of California Los Angeles",country:{name:"United States of America"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/219081/images/system/219081.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals. He is currently working on the protective activity of phenolic compounds in disorders associated with oxidative stress and inflammation.",institutionString:null,institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Dr.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. He has many years of experience in research and teaching in various fields of medicine, is an author/co-author of more than 200 scientific publications, 13 patents, 15 medical books/chapters, including Chapter in Book «Metabolomics», IntechOpen, 2020 «Metabolomic Discovery of Microbiota Dysfunction as the Cause of Pathology».",institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"199461",title:"Prof.",name:"Natalia V.",middleName:null,surname:"Beloborodova",slug:"natalia-v.-beloborodova",fullName:"Natalia V. Beloborodova",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/199461/images/system/199461.jpg",biography:'Natalia Vladimirovna Beloborodova was educated at the Pirogov Russian National Research Medical University, with a degree in pediatrics in 1980, a Ph.D. in 1987, and a specialization in Clinical Microbiology from First Moscow State Medical University in 2004. She has been a Professor since 1996. Currently, she is the Head of the Laboratory of Metabolism, a division of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russian Federation. N.V. Beloborodova has many years of clinical experience in the field of intensive care and surgery. She studies infectious complications and sepsis. She initiated a series of interdisciplinary clinical and experimental studies based on the concept of integrating human metabolism and its microbiota. Her scientific achievements are widely known: she is the recipient of the Marie E. Coates Award \\"Best lecturer-scientist\\" Gustafsson Fund, Karolinska Institutes, Stockholm, Sweden, and the International Sepsis Forum Award, Pasteur Institute, Paris, France (2014), etc. Professor N.V. Beloborodova wrote 210 papers, five books, 10 chapters and has edited four books.',institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"354260",title:"Ph.D.",name:"Tércio Elyan",middleName:"Azevedo",surname:"Azevedo Martins",slug:"tercio-elyan-azevedo-martins",fullName:"Tércio Elyan Azevedo Martins",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/354260/images/16241_n.jpg",biography:"Graduated in Pharmacy from the Federal University of Ceará with the modality in Industrial Pharmacy, Specialist in Production and Control of Medicines from the University of São Paulo (USP), Master in Pharmaceuticals and Medicines from the University of São Paulo (USP) and Doctor of Science in the program of Pharmaceuticals and Medicines by the University of São Paulo. Professor at Universidade Paulista (UNIP) in the areas of chemistry, cosmetology and trichology. Assistant Coordinator of the Higher Course in Aesthetic and Cosmetic Technology at Universidade Paulista Campus Chácara Santo Antônio. Experience in the Pharmacy area, with emphasis on Pharmacotechnics, Pharmaceutical Technology, Research and Development of Cosmetics, acting mainly on topics such as cosmetology, antioxidant activity, aesthetics, photoprotection, cyclodextrin and thermal analysis.",institutionString:null,institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"334285",title:"Ph.D. Student",name:"Sameer",middleName:"Kumar",surname:"Jagirdar",slug:"sameer-jagirdar",fullName:"Sameer Jagirdar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334285/images/14691_n.jpg",biography:"I\\'m a graduate student at the center for biosystems science and engineering at the Indian Institute of Science, Bangalore, India. I am interested in studying host-pathogen interactions at the biomaterial interface.",institutionString:null,institution:{name:"Indian Institute of Science Bangalore",country:{name:"India"}}},{id:"329248",title:"Dr.",name:"Md. Faheem",middleName:null,surname:"Haider",slug:"md.-faheem-haider",fullName:"Md. Faheem Haider",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329248/images/system/329248.jpg",biography:"Dr. Md. Faheem Haider completed his BPharm in 2012 at Integral University, Lucknow, India. In 2014, he completed his MPharm with specialization in Pharmaceutics at Babasaheb Bhimrao Ambedkar University, Lucknow, India. He received his Ph.D. degree from Jamia Hamdard University, New Delhi, India, in 2018. He was selected for the GPAT six times and his best All India Rank was 34. Currently, he is an assistant professor at Integral University. Previously he was an assistant professor at IIMT University, Meerut, India. He has experience teaching DPharm, Pharm.D, BPharm, and MPharm students. He has more than five publications in reputed journals to his credit. Dr. Faheem’s research area is the development and characterization of nanoformulation for the delivery of drugs to various organs.",institutionString:"Integral University",institution:{name:"Integral University",country:{name:"India"}}},{id:"329795",title:"Dr.",name:"Mohd Aftab",middleName:"Aftab",surname:"Siddiqui",slug:"mohd-aftab-siddiqui",fullName:"Mohd Aftab Siddiqui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329795/images/system/329795.png",biography:"Dr. Mohd Aftab Siddiqui is an assistant professor in the Faculty of Pharmacy, Integral University, Lucknow, India, where he obtained a Ph.D. in Pharmacology in 2020. He also obtained a BPharm and MPharm from the same university in 2013 and 2015, respectively. His area of research is the pharmacological screening of herbal drugs/natural products in liver cancer and cardiac diseases. He is a member of many professional bodies and has guided many MPharm and PharmD research projects. Dr. Siddiqui has many national and international publications and one German patent to his credit.",institutionString:"Integral University",institution:null}]}},subseries:{item:{id:"9",type:"subseries",title:"Biotechnology - Biosensors, Biomaterials and Tissue Engineering",keywords:"Biotechnology, Biosensors, Biomaterials, Tissue Engineering",scope:"The Biotechnology - Biosensors, Biomaterials and Tissue Engineering topic within the Biomedical Engineering Series aims to rapidly publish contributions on all aspects of biotechnology, biosensors, biomaterial and tissue engineering. We encourage the submission of manuscripts that provide novel and mechanistic insights that report significant advances in the fields. 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Finally, the tissue engineering subcategory will support topics such as the fundamentals of stem cells and progenitor cells and their proliferation, differentiation, bioreactors for three-dimensional culture and studies of phenotypic changes, stem and progenitor cells, both short and long term, ex vivo and in vivo implantation both in preclinical models and also in clinical trials.",coverUrl:"https://cdn.intechopen.com/series_topics/covers/9.jpg",hasOnlineFirst:!0,hasPublishedBooks:!0,annualVolume:11405,editor:{id:"126286",title:"Dr.",name:"Luis",middleName:"Jesús",surname:"Villarreal-Gómez",slug:"luis-villarreal-gomez",fullName:"Luis Villarreal-Gómez",profilePictureURL:"https://mts.intechopen.com/storage/users/126286/images/system/126286.jpg",biography:"Dr. Luis Villarreal is a research professor from the Facultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California, Tijuana, Baja California, México. 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