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Dr. Wei Wu has received awards from many national societies for the originality and quality of his projects. He has authored 70 peer-reviewed papers in international journals.",coeditorOneBiosketch:"A pioneering researcher in obstetrics and holder of three registered patents. Dr. Qiuqin Tang's research interests include genetic and epigenetic risk factors of reproductive and developmental health. She has authored over 20 papers in international journals.",coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"178661",title:"Dr.",name:"Wei",middleName:null,surname:"Wu",slug:"wei-wu",fullName:"Wei Wu",profilePictureURL:"https://mts.intechopen.com/storage/users/178661/images/system/178661.jpeg",biography:"Dr. Wei Wu is an associate professor and associate department\nchair in the Department of Toxicology, Nanjing Medical University, China, where he received his Ph.D. in Toxicology in 2012.\nHe was a guest researcher at the National Institute of Environmental Health Sciences (NIEHS) between 2017 and 2018. Dr.\nWu is a member of different national and international societies\nin the fields of human reproduction and toxicology and has\nreceived awards from many national societies for the originality and quality of his\nprojects. Dr. Wu has authored seventy-three peer-reviewed papers in international\njournals. He has edited four books and collaborated on ten others as well as seventeen patents and in the organization of three international conferences. He is a\nreviewer for ninety-eight journals.",institutionString:"Nanjing Medical University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"4",totalChapterViews:"0",totalEditedBooks:"4",institution:{name:"Nanjing Medical University",institutionURL:null,country:{name:"China"}}}],coeditorOne:{id:"184798",title:"Ms.",name:"Qiuqin",middleName:null,surname:"Tang",slug:"qiuqin-tang",fullName:"Qiuqin Tang",profilePictureURL:"https://mts.intechopen.com/storage/users/184798/images/13334_n.jpg",biography:"Qiuqin Tang is an attending doctor of The Women’s Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital). Her research interests include genetic and epigenetic risk factors of reproductive and developmental health. She has authored over 20 papers in international journals such as EBioMedicine, Clinical Epigenetics, Molecular Human Reproduction, Scientific Reports, and European Journal of Endocrinology. She has collaborated in four books and three patents. She is the Editor-in-Chief of Journal of Woman\\'s Reproductive Health, and editor of many other journals including Journal of Gynecology and Obstetrics, and Journal of Gynecology and Obstetrics Forecast.",institutionString:"Nanjing Medical University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"4",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Nanjing Medical University",institutionURL:null,country:{name:"China"}}},coeditorTwo:{id:"48837",title:"Prof.",name:"Panagiotis",middleName:null,surname:"Tsikouras",slug:"panagiotis-tsikouras",fullName:"Panagiotis Tsikouras",profilePictureURL:"https://mts.intechopen.com/storage/users/48837/images/system/48837.jpg",biography:"Dr. Panagiotis Tsikouras is a specialist in obstetrics-gynecology,\nperinatal medicine, and contraception at the School of Medicine,\nDemocritus University of Thrace, Greece. He is also the headmaster of the Family Planning Centre and Gynecological Cytology\nLaboratory at the same university. Dr. Tsikouras is a fellow of the\nInternational Academy of Clinical and Applied Thrombosis/Hemostasis. His scientific activities focus on paediatric and adolescence medicine, gynecological oncology, high-risk pregnancies. He is a reviewer for several international journals and has numerous scientific publications to his credit, including papers and book chapters. He has also contributed to international and national guidelines on coagulation and thrombosis in obstetrics-gynecology.",institutionString:"Democritus University of Thrace, Komotini",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"11",totalChapterViews:"0",totalEditedBooks:"1",institution:{name:"Democritus University of Thrace",institutionURL:null,country:{name:"Greece"}}},coeditorThree:{id:"290374",title:"Prof.",name:"Werner",middleName:null,surname:"Rath",slug:"werner-rath",fullName:"Werner Rath",profilePictureURL:"https://mts.intechopen.com/storage/users/290374/images/system/290374.jpg",biography:"Dr. Werner Rath is a specialist in obstetrics and gynecology, gynecologic oncology, perinatal medicine, and hemostaseology. He\nis currently a professor in the Gynecology and Obstetrics Faculty\nof Medicine, University of Kiel, Germany, and honorary doctor\nat the Democritus University of Thrace, Alexandroupoli University Hospital He previously served as chief of the Department\nof Gynecology and Obstetrics at University Hospital RWTH Aachen,\nGermany. Dr. Rath is a reviewer for numerous journals and chief editor of Geburtshilfe und Frauenheilkunde (GebFra). He has several publications, including thirteen\nbook chapters, to his credit.",institutionString:"Kiel University",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"4",totalChapterViews:"0",totalEditedBooks:"0",institution:{name:"Kiel University",institutionURL:null,country:{name:"Germany"}}},coeditorFour:{id:"299669",title:"Prof.",name:"Georg-Friedrich",middleName:null,surname:"Von Tempelhoff",slug:"georg-friedrich-von-tempelhoff",fullName:"Georg-Friedrich Von Tempelhoff",profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:"St. Vinzenz Krankenhaus",position:null,outsideEditionCount:0,totalCites:0,totalAuthoredChapters:"1",totalChapterViews:"0",totalEditedBooks:"0",institution:null},coeditorFive:null,topics:[{id:"16",title:"Medicine",slug:"medicine"}],chapters:[{id:"79159",title:"Open Fetal Surgery and Fetoscopic Repair in Spina Bifida and Myelomeningocele in Romania",slug:"open-fetal-surgery-and-fetoscopic-repair-in-spina-bifida-and-myelomeningocele-in-romania",totalDownloads:61,totalCrossrefCites:0,authors:[null]},{id:"79947",title:"Endoscopic Approach to Ectopic Pregnancy",slug:"endoscopic-approach-to-ectopic-pregnancy",totalDownloads:59,totalCrossrefCites:0,authors:[null]},{id:"80212",title:"Diagnosis of Ectopic Pregnancy",slug:"diagnosis-of-ectopic-pregnancy",totalDownloads:74,totalCrossrefCites:0,authors:[null]},{id:"80756",title:"Medical Management of Ectopic Pregnancy",slug:"medical-management-of-ectopic-pregnancy",totalDownloads:32,totalCrossrefCites:0,authors:[null]},{id:"81269",title:"Fetal Craniospinal Malformations: Aetiology and Diagnosis",slug:"fetal-craniospinal-malformations-aetiology-and-diagnosis",totalDownloads:13,totalCrossrefCites:0,authors:[null]},{id:"81570",title:"Prenatal Diagnosis of Diaphragmatic Hernia",slug:"prenatal-diagnosis-of-diaphragmatic-hernia",totalDownloads:15,totalCrossrefCites:0,authors:[null]},{id:"81868",title:"Prenatal Diagnosis: The Main Advances in the Application of Identification of Biomarkers Based on Multi-Omics",slug:"prenatal-diagnosis-the-main-advances-in-the-application-of-identification-of-biomarkers-based-on-mul",totalDownloads:1,totalCrossrefCites:0,authors:[null]},{id:"81273",title:"Ectopic Pregnancy after Ipsilateral Salpingectomy",slug:"ectopic-pregnancy-after-ipsilateral-salpingectomy",totalDownloads:13,totalCrossrefCites:0,authors:[null]}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},personalPublishingAssistant:{id:"278926",firstName:"Ivana",lastName:"Barac",middleName:null,title:"Ms.",imageUrl:"https://mts.intechopen.com/storage/users/278926/images/8058_n.jpg",email:"ivana.b@intechopen.com",biography:"As an Author Service Manager my responsibilities include monitoring and facilitating all publishing activities for authors and editors. From chapter submission and review, to approval and revision, copyediting and design, until final publication, I work closely with authors and editors to ensure a simple and easy publishing process. I maintain constant and effective communication with authors, editors and reviewers, which allows for a level of personal support that enables contributors to fully commit and concentrate on the chapters they are writing, editing, or reviewing. I assist authors in the preparation of their full chapter submissions and track important deadlines and ensure they are met. I help to coordinate internal processes such as linguistic review, and monitor the technical aspects of the process. As an ASM I am also involved in the acquisition of editors. 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\n\t\t\t
1. Introduction
\n\t\t\t
Colloquially speaking, turbulence in any language means disorderly, incomprehensible, and of course, unpredictable movement. Consequently, we encounter expressions that employ the word turbulence in social and economic contexts; in aviation whenever there are abnormalities in the air, and even in psychology and the behavioural sciences in reference to turbulent conduct, or a turbulent life, in the sense of a dissolute existence. Thus has the word turbulence become associated with chaos, unpredictability, high energy, uncontrollable movement: dissipation. All of the foregoing concepts have their source in the world of hydrodynamics, or fluid mechanics.
\n\t\t\t
In fluid mechanics, turbulence refers to disturbance in a flow, which under other circumstances would be ordered, and as such would be laminar. These disturbances exert an effect on the flow itself, as well as on the elements it contains, or which are submerged in it. The process that is taking place in the flow in question is also affected. As a result, they possess beneficial properties in some fields, and harmful ones in others. For example, turbulence improves processes in which mixing, heat exchange, etc., are involved. However, it demands greater energy from pumps and fans, reduces turbine efficiency and makes noise in valves and gives rise to vibrations and instabilities in pipes, and other elements.
\n\t\t\t
The study of turbulence and its related effects is a mental process; one that begins with great frustration and goes on to destroy heretofore accepted theories and assumptions, finally ending up in irremediable chaos. “I am an old man now, and when I die and go to heaven, there are two matters on which I hope for enlightenment. One is the quantum electrodynamics, and the other is the turbulent motion of fluids. An about the former I am rather optimistic” (Attributed to Horace Lamb).
\n\t\t\t
Nonetheless, some progress has been made in turbulence knowledge, modelling and prediction. (Kolmogorov, 1941). This chapter will deal briefly with these advances, as well as with the effects of turbulence on practical applications. In this sense, reference will be made to noise effects and modelling, as well as to flow vibration and instabilities provoked by turbulence. (Gavilán 2008, Gavilán 2009).
\n\t\t
\n\t\t
\n\t\t\t
2. Turbulence.
\n\t\t\t
Of itself, turbulence is a concept that points to unpredictability and chaos. For our purposes, we will deal with this concept as it applies to fluid mechanics. Therefore, we will be dealing with turbulent flow. Throughout what follows, the terms turbulence and turbulent flow will be understood as synonymous. Some texts treat the terms turbulence and vortex as analogous, however, this seems to be rather simplistic. For the purposes of this work, it seems best to take the concept of turbulence in its broadest sense possible.
\n\t\t\t
Historically, fluid mechanics has been treated in two different ways, namely, in accordance with the Euler approach, or pursuant to the Lagrange approach. The Eulerian method is static, given that upon fixing a point, fluid variations are determined on the effect they have on this point at any given time. On the other hand, the Lagrangian method is dynamic, given that it follows the fluid. In this way, variations in the properties of the fluid in question are observed and/or calculated by following a particle at every single moment over a period of time.
\n\t\t\t
The Eulerian method is the one most employed, above all, in recent times, by means of numerical methods, such as that of finite elements, infinites, finite volumes, etc. Notwithstanding, there is great interest in the Lagrangian method or approach, given that it is one that is compatible with methods that do not use mesh or points. (Oñate, E; et al. 1996)
\n\t\t\t
Throughout history, there have been two currents of thought as regards the treatment of turbulence. One is the so-called deterministic approach, which consists of solving the Navier-Stokes equation, with the relevant simplifications, (Euler, Bernoulli) practically exclusively via the use of numbers. The other approach is statistical. The work of Kolmogorov stands out in this field; work which will be dealt with below, given its later influence on numerical methods and the results of same. Apart from the Eulerian or Lagrangian methods, classic turbulent fluid theory will be dealt with in Section 2.1, whereas the statistical or stochastic approach will be dealt with in Section 2.2, in clear reference to Kolmogorov’s theory. (Kolmogorov, 1941).
\n\t\t\t
\n\t\t\t\t
2.1. General theory
\n\t\t\t\t
This section provides a brief and concise exposition of successive fluid flow approaches designed to respond to the presence of anomalies that were later referred to as turbulence, and which gave rise to the concept of turbulent fluid. Furthermore, the equations given enable the visualisation of the turbulence in question and its later development. A Eulerian and deterministic focus will be followed in this section.
\n\t\t\t\t
Working in reverse to the historical approach, the fluid flow equation formulated by Navier-Stokes in 1820 is given; firstly, because it is the most general one, and secondly, because, of itself, its solution can represent the turbulent flow equation.
Where u\n\t\t\t\t\ti stands for the velocity components at each point and at each moment in time, υ is the viscosity, p is the pressure at each point and at each moment in time, and f\n\t\t\t\t\ti refers to the external forces at each point and at each moment in time. By annulling the viscosity and its effects, we get Euler’s fluid flow equation announced in 1750.
The solving of these equations, given their simplifications and context conditions, provide us with a field of speeds and pressures for a fluid in movement. Indeed, as regards the Navier-Stokes equation, it can be solved and turbulences and instabilities determined by employing powerful numerical solution methods, such as that of the finite element.
\n\t\t\t\t
Thus, the equations that govern fluid movement. By means of these two equations, particularly the last two (those of Euler and Bernoulli), it was observed that, under certain conditions, the results did not correspond to reality, on account of a certain problem of disorder developing in the fluid and its flow. Only the accurate and numerical solving of the Navier-Stokes equation can exactly reproduce these phenomena. To this end, resort must be had to potent computational fluid dynamic (CFD) software. Notwithstanding, in 1883, Osborne Reynolds discovered a parameter that predicted or anticipated the chaotic and turbulent of the fluid: the Reynolds number.
Thus was it established that the flow is stationary, and therefore laminar, for Re<2000 values, a fact which meant that the solutions given by Bernoulli and Euler were very accurate. For values of 2000<Re<4000 the system was deemed to be in transition, and therefore, not stationary. The functions that work best are those of Euler and Navier-Stokes. This turbulence undergoes three phases or states of development.
\n\t\t\t\t
đ Growth of coherent bidimensional vortices\n\t\t\t\t\t\t\t\t
These are referred to as coherent because the vorticity is concentrated and the fluid flows around as if it were a solid obstacle. It keeps its shape for longer than a single rotation.
\n\t\t\t\t\t\t\t.
đ Joining of Vortices
đ Separation of vortices and turbulent state in 3D
\n\t\t\t\t
Finally, if completely turbulent, the fluid is non-stationary and tri-dimensinal for values of Re>4000. These solutions are only possible by means of using the Navier-Stokes equations.
\n\t\t\t\t
In conclusion, only the numerical solution of the Navier-Stokes equation provides a solution that considers turbulence. Nevertheless, there are other processes and approaches that will be developed in Section 2.3. Turbulence is, therefore, produced by the interaction of the fluid with geometry, by the loss of energy due to viscosity, by density variations caused by temperature, or other factors, such as changes in speed, or all of these at once. Consequently, the turbulent flow is unpredictable and chaotic in the sense that it depends on a host of small variations in the initial conditions and these disturbances are amplified in such a way that it becomes possible to predict them in space and time. Another of its features is its great capacity for mixing and, lastly, that it affects at various scales and wavelengths. It could be said that together, fluid, structure and context conditions, constitute a non-linear, non-stationary dynamic system. Its most noteworthy characteristic is its sensitivity to the initial conditions and its self-similarity, which will serve as a staring point to develop Kolmogorov’s theory.
\n\t\t\t\t
In the 19th and 20th centuries, several researchers devoted great efforts to studying turbulence under certain, extremely particular conditions. Examples of such turbulence or instabilities are to those of Von Karman, Kevin-Helmotz, Raleygh-Bernard, and so on.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
2.2. Kolmogorov´s theory
\n\t\t\t\t
Kolmogorov’s theory cannot be dealt with without first mentioning the spectral analysis of turbulence, or the application of Fourier\'s analysis to the study of turbulence. Fourier’s theory decomposes the fluctuations into sinusoidal components and studies the distribution of the turbulent energy along several wavelengths. In this way it becomes possible to get several scales of turbulence and their evolution in time. This technique works and produces acceptable results when turbulence is homogenous. Under this condition, accurate equations can be determined for the speed spectrum and for the transferring of energy between difference scales of turbulence, as well as the dissipation of turbulent energy due to viscosity. The simplest development assumes that there is no average speed gradient, in such a way that the turbulence interacts with itself, with the energy dropping by itself. Neither energy sources nor sinks are taken into account.
\n\t\t\t\t
To start, we assume that the speed of a particle in the fluid can be decomposed into an average speed plus a fluctuation component.
Therefore, we assume that the value of the correlation function tends to zero when the radius tends to the infinite. We now define a spectral function Ф\n\t\t\t\t\tij as the Fourier transform of the correlation function in 3D:
This spectral function, which will form the spectral matrix, depends on time and on the wave number k, which is a vector. The turbulent kinetic energy can be expressed as:
The magnitude of the vector of the wave number k=[k] is the inverse of the length, thus the length is k\n\t\t\t\t\t-1. Therefore, if L is a characteristic scale of turbulence, the L\n\t\t\t\t\t-1 value of the wave number represents the higher scales of turbulence, where all the turbulent kinetic energy is contained. The spectral function, Ф\n\t\t\t\t\tij, describes the energy distribution in relation to wave numbers. It has a maximum value of [k]=L\n\t\t\t\t\t-1, which drops to zero when [k] tends to the infinite. The evolution of kinetic energy is defined as:
As far as the evolution of the k energy value is concerned, this can be seen in the following figure (Figure 1).
\n\t\t\t\t
Figure 1.
Energy ranges.
\n\t\t\t\t
This is where the term, or value, η comes in, referring to Kolmogorov’s scale, which determines the scale point at which energy dissipation by viscosity is most significant. We will use the following equation for Kolmogorov’s theory:
That is to say, the energy and its evolution in time will be a function of that which is transferred and that which is dissipated. Integrating it from k=0 to a greater, or finite, as well as limited, value gives us:
“Energy changes in wave numbers below k on account of the interchange with larger wavelengths through the energy spectral fluid and the dissipation caused by viscosity forces on its own wavelength”
\n\t\t\t\t
Kolmogorov holds that turbulence properties in the inertial range, above η, only depend on the energy spectral fluid, in such a way that:
The idea behind this is that the transfer cascade evolves towards lower scales, leaving the spectral fluid as a fixed parameter. Consequently, the energy equation now stands at:
where C is a Kolmogorov constant, considered as a universal, the value of which is 1.5 when L\n\t\t\t\t\t-1<<k<<η\n\t\t\t\t\t-1 and the Re (Reynolds number) is high. Thus, beyond the inertial scale wave numbers, we have the dissipation range numbers, as shown in Figure 2.
\n\t\t\t\t
Figure 2.
Kolmogorov scales and data from several tests.
\n\t\t\t\t
This range is only determined by the η parameter, which only has a dependence parameter, as shown by the equation (21).
We will define the Kolmogorov time and speed scale factor with the ε y υ parameters, which determine the behaviour of the flow in the dissipation range:
Thus, the Kolmogorov theory completes the spectral analysis. The theory postulates high Reynolds number values. The small turbulence scales are assumed that serve to balance and be controlled by the average energy flow, which is generated in the inertial scale and which equals the dissipation rate. Furthermore, Kolmogorov’s theory universally predicts speed properties and their differences for small separations, as well as their correlations and spectre, only depending on the υ y ε parameters. Kolmogorov also marked the boundary between the transferred or contained energy range (inertial range) and the dissipative structures by way of the following expression:
Therefore, given a Reynolds number, the lower scales are not sensitive to the turbulent flow in which they find themselves. Nevertheless, the lower scales are intimately related to the flow, with their properties varying substantially depending on the specific flow. These concepts will form the basis for future methods of modelling and solving fluid movement problems, such as k-ε and Large Eddy Simulation (LES) models.
\n\t\t\t
\n\t\t\t
\n\t\t\t\t
2.3. Simulation of turbulent fluids.
\n\t\t\t\t
As is well known, Navier, L.H.H. and Stokes, G.G derived fluid movement equations over 150 years ago. This equation, along with that of continuity, provides an answer to any fluid movement problem.
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The solution and determination of the speed field is, therefore, nothing more than discretising the domain and the differential equations and applying context conditions in order to repeatedly solve the system formed until achieving convergence. This is the so-called Direct Numerical Solution (DNS). Nonetheless, this simple method is only useful in simple geometries, given that otherwise, the time calculation would be so big as to make any simulation unfeasible. This defect arises noticeably when we design fluid-structure (FIS) models. Consequently, other models need to be found, which are lest costly computationally speaking.
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The most common solution to the high number of elements required by the former simulation method, DNS, is to use weighted, or weighting, techniques. In this way, modelling is done on a small scale expecting that the solution will respond to the flow as a whole. This is the idea that underlies the so-called Reynolds Averaged Navier-Stokes (RANS). It must be assumed that the speed of a turbulent flow can be described as follows:
That is to say, as the sum of an average speed plus a fluctuating component on this average speed. The following is an example of the application of this assumption. It refers to the 2D modelling of a turbulent flow on a flat plate, the Navier Stokes (NS) equation for which is given below:
known as Reynold Stress. In order to avoid this difficulty, the kinetic energy (k) and the dissipation rate (ε) are modelled. This gives rise to a well known model: the k- ε model.
This k- ε model has a big advantage over the DNS model in that it takes less time to compute. This model provides acceptable solutions when flow fluctuations are not very important. When the flow motor, or the problem, to be studied has to do with pressure fluctuation, that is to say, external flow Flow Induced Vibrations (FIV), the RANS model is unviable, given that it does not provide quality solutions. There are several RANS model variations, the main features of which are shown in Table 1.
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\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Method
\n\t\t\t\t\t\t\t
Strengths
\n\t\t\t\t\t\t\t
Weaknesses
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
Spalart- Allmaras
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A one-equation model, which provides less computational effort than most other models. Producet for external flow over aitfoils but is increasing in popularity for turbo machinery applications. Performs well for attached wall-bounded flows with weakly complex boundary layers
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Weak for adverse pressure gradients that produce boundary layer separation. Since it is relatively new, it has a lack of submodels available.
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\n\t\t\t\t\t\t\t
RNG k-ε
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Possesses many of the same characteristics as the standard k- ε, but uses mathematical group theory to detrmine the previously empirical constans. It preforms better for moderately complex flows like jet impingement
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Subject to limitations due to isotropic eddy viscosity assumptions
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Reynolds Stress Model (RSM)
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Highly rooted in the physics by solving a transport equation for each Reynolds stress.
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Requires much more computational effort than any other technique.
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Table 1.
RANS submodels, strengths and weaknesses.
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Lastly, there is a model that lies halfway between the RANS and DNS. The DNS model has been seen to represent the real circumstance and disturbances very well. On the other hand, though the RANS model is extremely comfortable computationally speaking, it does not represent turbulence or disturbances very well. The so-called Large Eddy Simulation (LES) model provides instantaneous solutions, as does the DNS model, while at the same time containing models and simplifications such as those in the RANS model. The LES model solves the Navier-Stokes equation as does the DNS model, but the equations are spatially filtered, or refined. This filtering or refining of the equations means that the flow is determined at a characteristic scale, and is modelled afterwards at lower scales. It is the application of Kolmogorov’s theory to non-stochastic numerical models.
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This succession of modelling and refining is due to the fact that big eddies behave anisotropically and, therefore, must be calculated, whereas smaller eddies behave isotropically and can, as such, be treated statistically. Thus, the mesh is such that the majority of energy is contained in big eddies and calculated directly, the rest is assigned in a weighted manner to flow as a whole.
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The bigger the Reynolds number, the bigger the cost in this LES model. Mathematically, the model is based on the Ф flow being formed by two superimposed flows:
the mesh flow, or the grand scale flow,\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\tϕ\n\t\t\t\t\t\t\t\t\t\t¯\n\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t
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the sub-mesh flow, or the small scale flow\n\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\tϕ\n\t\t\t\t\t\t\t\t\t\t\'\n\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t
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The grand scale (GS) flow is calculated as follows:
the latter term is modelled on the sub-mesh, or on the SGS sub-model and the accuracy of the model rests on the idea that lower speeds than the mesh are homogenous and, therefore, can be modelled with great accuracy. The aim of the LES method is to solve the majority of the flow and to model only a small part of it. Thus, the LES method strikes a balance between mesh size and accuracy. Figure 3, in which the solution for a turbulent flow in a pipe compares the RANS method with the LES one, provides an explanatory example.
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These are practically the general models that are used in flow simulation and fluid movement. Notwithstanding, there are variations of these basic methods depending on the application in question.
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Figure 3.
Solutions of the same problem with a RANS model (on the left) and LES model (on the right).
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Quite clearly, discretisations can be Eulerian, or Lagrangian, in such a way as to respond to both forms of tackling problems concerning fluid dynamics.
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3. General effects of the turbulence. (FIC)
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Having introduced turbulence, the turbulent flow and its equations and simulations, the question arises as to what can turbulence do? What are its possible effects? To answer these questions, first off it is necessary to decide whether the effects in question are general or local, that is to say, whether they are provoked by the turbulent flow or, on the contrary, are caused by turbulence or instabilities. The latter will be dealt with in Sections 4, 5 and 6, while the general effects are considered below.
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Numerous studies have been carried out highlighting the benefits of turbulence with respect to miscibility, diffusion and heat exchange, though it can also have harmful effects. Here we are going to deal with the phenomenon of spontaneous cavitation, and its effects, in turbulent flow. Specifically, our aim is to study the effect of the change of speed caused by vorticity and pressure due to load loss on account of turbulence in high energy turbulent fluids near saturation point, such as, for example, feed water in power stations.
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Firstly, we are going to define a non-dimensional parameter, which is called the cavitation number.
\n\t\t\t\tP\n\t\t\t\ta is the local or system pressure;
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\n\t\t\t\tP\n\t\t\t\tv is the vaporization pressure at system temperature;
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\n\t\t\t\tΡ is the fluid density at system temperature;
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\n\t\t\t\tV is the fluid speed.
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Cavitation will occur when the parameter value is low. There is a limit value that corresponds to a determined speed, for each temperature and pressure, below which cavitation is ensured.
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Speed can be defined in a turbulent flow as follows:
If we assume that δu(t) is proportional to the standard deviation of the measured values in the speed system, and that in turn, this standard deviation is a function of the average speed, (Gavilán, C.J. 2008) we find that:
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\n\t\t\t\t\t\tδu(t) is zero for low speeds, therefore, the Y expression is accurate and can be applied to all fluid points. Moreover, the Bernoulli and Euler theorem is applicable, therefore, there will be no spontaneous cavitation, unless the average speed changes.
For high speeds, δu(t)>>0, there may be some speed values at which Ca is lower than the average value, and therefore, spontaneous cavitation may occur.
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This effect will be called Fluid Induced Cavitation (FIC), given that, although cavitation is associated with turbulence, in turbo-machines or rotary pumps, little attention is paid to spontaneous cavitation due to the effect of turbulence on fluid systems. This effect is not widely known, though it is most definitely of great importance when dealing with fluids that are working very near the vaporization limit, or to put it more clearly, when the system pressure is very near the fluid vapour pressure at working temperature. This is particularly important for thermal power plants, or energy producing stations. In such facilities, the liquid is heated before entering the vaporization element; if there are elements that make the pressure fall, such as elbows, T\'s, filters, etc. in the conditions prior to vaporization, the numerator of equation 39 drops, reducing the Ca value, thus representing a high risk situation. In the same way, if we have elements in which the speed rises, such as jet pumps, venturi tubes, and other restrictions, the denominator increases, in such a way that the Ca value falls, thus increasing the risk of cavitation.
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Figure 4.
Flow in pipe system.
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Cavitation, which elsewhere and in other fora is referred to as flashing, is a vibration and noise - FIV and FIN - and may even manage to change the flow pattern, and therefore, cause FII flow instabilities. Other effects also need to be taken into account, such as the presence of erosion-corrosion, in what is, in principle, a single-phase fluid. Furthermore, if there are stable cavitation conditions, and a real and permanent void ratio is established, pump cavitation is ensured, even when this should not occur according to the calculations.
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There are other reasons for possible cavitation, such as variations in the density and vapour pressure parameters on account of changes in the pressure and temperature conditions.
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Let us have a look at a real example. Figure 4 shows the case of feed water speed in a pipe to an electricity-generating installation boiler. If we calculate the Ca parameter between two points, A and B, already having the average, or tendency, speed and the real one, we see that their Ca values are different. After the Ca, v and p values have been calculated in several situations, if we extrapolate them, there is such a turbulent speed value that it provokes spontaneous cavitation (Figure 5). Therefore, there is a speed limit for each flow at which spontaneous cavitation occurs, which coincides with the asymptotic value.
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Figure 5.
Cavitation number versus flow speed.
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The cavitation effect is not completely harmful, given that occasionally it is provoked by means of the speed of the fluid around a vehicle in such a way that movement resistance is very low and energy is saved in the movement, the fluid entry length is increased, or the energy is increased on knocking against another object in the fluid. This is the case of the supercavitating torpedoes used by the Russian navy.
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4. Fluid Induced Vibration. (FIV)
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There are lots of books and articles that have dealt with this subject. Strictly speaking, the term was only coined in the 1980’s. In truth, this case study is a particular case of fluid-structure interaction. Interest in this subject lies in the fact that the source of vibration is dissipated energy caused by turbulence, or in other cases, by eddies that produce oscillating lift forces that impregnate objects immersed in the fluid with a vibratory movement.
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There are two basic FIV mechanisms:
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a self-induced vibrating mechanism
a forced vibration mechanism.
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Mathematically speaking, this is true. Nevertheless, the subject is somewhat wider than this might suggest, as can be seen from the following classification, which provides us with a more complete view. (Figure 6).
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Figure 6.
Vibration mechanism.
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As far as we are concerned, two of the most important effects, or examples, as regards nuclear plants of the Boiling Water Reactor (BWR) type, are those that reflect the influence of FIV on reactor internals. That is to say, on single-phase fluids in a non-stationary operating system with turbulent flow, and in the case of a bi-phase flow, those that are subject to FIV on account of the vibrations caused by the phase change, which may even go as far as to cause thermo-hydraulic instabilities.
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Therefore, we will analyse two examples, fuel element vibrations in a flow with phase change and vibration induced by the leak flow in a BWR’s jet pumps.
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In the latter case, the jet pump FIV can be modelled as a vibration induced by the external axial flow. This is so because the leak flow through the Slip-Joint is deemed to be external to the mixer, as can be seen from Figure 7.
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Figure 7.
JetPump and Slipjoint leak.
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Thus, the model will be an axial flow cylinder, as described by Chen and Wambsganss (1970). The turbulent flow in the exterior, axial fluid gives rise to uneven pressure distribution on the outer wall of the pipe. Thus, on lacking balance it possesses resulting transverse forces. Moreover, as it is neither a permanent nor a stationary situation, the continuous change in pressure distribution over time, in space and in axial length, produces vibrations. The situation described is that which is shown in Figure 8.
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Figure 8.
Pressure distribution in an external flow pattern.
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The Corcos (1963) model is used to analyse this type of situation. The model is expressed by:
where ψ\n\t\t\t\tpp is the cross-spectral density of the pressure field, φ\n\t\t\t\tpp is the pressure power spectrum at the point, A and B are spatial functions and V\n\t\t\t\tc is the convection speed.
These equations result in the generation of transversal forces, which move the mixer parts, the elbows and the riser. Moreover, as a result of its geometrical configuration, mode 3 vibration causes damage as a result of the stress at the first fixed point of the system, which is the joint between the riser and its support. Indeed, it is this stress that provokes breakages. A detail of the turbulence associated with the simulation of slipjoint leak can be seen in Figure 9.
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In general, the Païdousis (1973) model is the equation used for industrial settings.
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As far as FIV caused by external flow with phase change is concerned, its study and development can basically be put down to the nuclear industry, after the development of boiling water reactors. At such plants, boiling occurs on the external part of fuel rods in the upflow. This study was later extended to the steam generator pipes of pressurised water reactors. Figure 10.
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Figure 9.
Simulated Slipjoint leak and vortex induced.
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Figure 10.
Fuel Bundle and Steam Generator.
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Both studies reached the same conclusion, namely that the vibration amplitude is proportional to the mass upflow, but decreases with pressure. As far as fluid quality, or the void ratio, is concerned, two peaks can be seen, 0.1-0.25 and 0.4-0.5, which suggests that there is great dependence between vibration amplitude and the void ratio, or fluid quality.
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On a BWR (Figure x) type fuel bar, it was established that the pressure power spectrum of the excitation force is proportional to V1.56-2.7, while the amplitude is proportional to V0.78-1.35. These values were determined in a rod test carried out by Pettigrew and Taylor [13] under the following conditions:
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Pressure: 2.8-9 MPa
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Mass flow: 0-4600 Kg/m2s
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Power: 1-1000 Kw
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Quality: 0-0.25.
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\n\t\t\t\tSaito (2002) came to the same conclusions in another test under different conditions.
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5. Fluid Induced Noise (FIN)
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In this section, we will take a look at the noise generated by a turbulent flow. Throughout this chapter and the following sections, fluid dynamics is based on movement equations, with speed as the unknown, essential analysis value.
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For this particular case of noise induced by turbulence, a change of mindframe is called for in the sense of assessing the fluid in terms of pressure, and even density. Original FIN theory and expressions are given by the Ligthhill aeroacoustic model (1952/1954), as indicated below:
where T\n\t\t\t\tij is the turbulent stress tensor. Whenever the fluid can be compressed, pressure variation is accompanied by density variation, the expression of which is:
Thus, turbulence collaterally generates pressure and density variation in the fluid. By means of the turbulent stress tensor the turbulence produces variations in pressure and density. The former cause the noise, and as such, are deemed to be sound sources.
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There are many practical applications of the analysis of turbulence generated noise. Two particularly curious, albeit useful ones, have solved some serious problems. The two situations in question are:
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Determination of leaks through the seat of safety relief valves from the outside by means of non-intrusive techniques.
Element breakage due to resonance frequencies.
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The first of the above situations has been used to detect safety relief valve leaks in BWR nuclear plants. The theoretical principle employed is that the seat leak flow produces turbulence which in turn generates a characteristic sound. The conceptual model is similar to the one used by Van Herpe and Creghton (1994), in which they model the fluid flow through a conduit with a restriction inside. These researchers came to the conclusion that the acoustic power, dB, is proportional to the speed in the conduit and to the passage diameter determined by the restricting element. Consequently, an SRV leak can be detected by means of sound and its register, but also by its evolution. The latter claim is based on the tests done by EPRI on this particular study NP-2444-SY. In this study, the frequency band is established at which the leak is best detected, 40-55 KHz, as well as the complete detection interval, 30-60KHz. Likewise, the author holds that regular monitoring can establish drift and trend patterns, the development mathematical law of which is that determined by Van Herpe y Creghton (1994).
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The other case refers to the catastrophic breakages of steam dryers at BWR plants due to an acoustically sourced resonance. (Figure 11).
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After the failure of the Quad cities dryer, a study was carried out on the loads to which it was subject. In none of the cases did the operating loads justify the breakage, or degradation, of the component. Consequently, a study was made of the vibratory or pulsatory phenomena, by measuring the vibrations at the component and the passage of pressure along the main steam lines. Acoustic excitation coming from the main steam lines is the current model. (Figure 12).
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Figure 11.
BWR vessel and Steam dryer detail. (Cracked areas)
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Figure 12.
MSL, SRV’s and the main characteristic of the sound transmission.
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Acoustic excitation is produced in the main steam lines as a result of the turbulence generated at the SRV connections, instrumentation connections, equalising header, etc… (Figure 13)
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This turbulence, which in general, manifests itself as vortices or eddies will, in the Von Karman sense, give off a determined frequency. Moreover, the lower scale turbulence will have its own frequency in accordance with the Lighthill model. Thus, the pressure waves that are generated with a defined frequency will constitute an external dryer load that increase its total dynamic load. (Figure 14).
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Figure 13.
Singing branch. Vortex Sheeding.
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Figure 14.
Frequency dependency of the power and acceleration of the induced load.
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Moreover, this load with be pulsatory in nature and will have a determined frequency, thus, if it coincides with a natural dryer frequency, the pressure effects are amplified, exceeding the resistance of the material, in some cases breaking, in others causing cracks.
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As can be seen, there are frequencies in Figure 14 that coincide with certain natural ones of parts of the dryer (Figure 15). Consequently, there will certainly be damage. The only variable will be time and the power of the vibration due to the reactor power. Notwithstanding, the solution to the problem is to be found at the same source as the excitation, namely, by studying the turbulence. It has been proven that by changing the geometry of the SRV connection, this changes the spectrum frequency, and consequently, the effect on the dryer. (Figure 16).
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In line with the practical focus followed in this chapter, there are reduced analytical studies that make it possible, in a T-type geometry, to predict and check the existence of acoustic excitation applied to steam pipes.
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Figure 15.
Natural frequencies of the steam dryer Hood and skirt.
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Figure 16.
Acoustic side branch, and the effect on the pressure, or load.
\n\t\t\t\ta is the speed of sound in a fluid medium;
\n\t\t\t
\n\t\t\t\tN takes on value of 1 and 3;
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\n\t\t\t\td is the branch diameter;
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\n\t\t\t\tr is the branch curve radius at its connection to the main line;
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\n\t\t\t\tL is the branch length;
\n\t\t\t
\n\t\t\t\tV\n\t\t\t\t0 is the main line speed.
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The assessment is carried out in accordance with the Strouhal number, defined by the equation x, if the value lies within the 0.25-0.60 interval, for which it can be said that there is a big likelihood of acoustic excitation appearing. The frequency estimate can be approximated by the following function:
In this section, we are going to deal with fluid instabilities, though more specifically with that which makes the flow bistable, given that this represents a transition within the turbulent system. Notwithstanding, what follows is a brief description of other instabilities about which references and studies abound. Turbulent flow, as has been above, is a generalised process, whereas instability is different. This is an unexpected situation, and one which, in principle, should not be happening. Quite often it is associated with the local formation of turbulence in a laminar, ordered flow. The most widely known instabilities model and study turbulence of this type of situation, but there are other instabilities in a turbulent flow that cause changes to the flow pattern. As with turbulent flow and its transition, instabilities possess factors that give rise to same, which are:
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Fluid-structure interaction: Remodels the flow
Von Karman
Stroudhal
Density or viscosity variations
Raleigh-Taylor
Plateau-Railegh
Ritchmyer-Meshkov
Speed difference
Kevin-Heltmotz.
Increase in turbulence intensity
Bistable flow.
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All of these instabilities exert different effects on the structure that is either immersed in them, or that contains the flow, such as vibrations, noise or change in process flow values. The latter, is of itself only an instability, albeit a very perculiar one, thus it will be dealt with separately. The others, given their spatial characteristics, have been dealt with separately in Sections 4 and 5.
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Presented here is an important advance on the investigation of the bistable flow phenomenon inside a boiling water reactor (BWR) vessel. The study of the flow time series concludes that the phenomenon of the bistable flow is a particular case of transitions induced by turbulence. Afterwards the phenomenon is identified, simulated and the model is validated. The real behaviour is reproduced using the mathematical model, which has given excellent results. Therefore the bistable flow is an instability that comes by transitions induced by turbulent flows and vortex.
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During this analysis a new technique was used to characterize the phenomenon. The new technique was the Hilbert´s transform. The conclusion that came from this last study is that:
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The bistable flow is non linear and non stationary
The bistable flow is produced when the plant is near the coast down and the flow is the maximum
There is a relationship between the average value of the flow and the bistability.
The bistable flow has two attractors structure.
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Figure 17.
Flow rate time series of in the recirculation loop. Bistable event.
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One of the most absurd process features is that the white noise can induce order in a system that is non linear and non stationary, is not in equilibrium. That is, a chaotic system can be ordered by itself. This is called vibration resonance (Horsthemke, 2005), with transitions induced by noise. In this case the word noise and turbulence will be similar. So this phenomenon is modeled by the Laugevin´s equation (56).
ε(t) it is a gaussian noise zero-averaged an non correlated.
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Figure 18.
Results of the Hilbert´s Transform. Real Time series (central), Phase (bottom), Inst. Frequency (upper)
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The last terms in the equation, represent the external and periodic forces represented as low frequency and amplitude A, and another of high frequency with amplitude B.
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For the case under study the expressions of the functions g(x) and f(x) are:
x(t) is the flow and ζ is the zero averaged and non-correlated noise white. The parameters K1\' and. K3 will be calculated to determine the times in those that, statistically, the system is in each value, or also called escape times.
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To determine the values of the K´s factors it must be consider that:
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The averaged value of the flow is 5650 t/h and if there´s no noise or turbulence the results of the equation (61) are zero.
The averages deviations of the average value must be +50 and -50 t/h.
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The values of the parameters expressed in the equation (61) and that are coherent with the previous premises are given by the table 2.
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\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Parameter
\n\t\t\t\t\t\t
Value
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
K1`
\n\t\t\t\t\t\t
0.0052
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
K2
\n\t\t\t\t\t\t
8e-11
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
η
\n\t\t\t\t\t\t
0.1797
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Desv. Typical Noise
\n\t\t\t\t\t\t
40
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\n\t\t\t\t
Table 2.
Values of parameter to equation (58).
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The realization of the equation (61), gives results such the follows, showed in figure 19 and 20.
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Figure 19.
Realization of the equation (61) with the parameters of the table 2.
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Figure 20.
Realization (2nd) of the equation (61) with the parameters of the table 2.
\n\t\t\t
The previous graphics are two drawings of the flow value in sets of 2000 samples, in order to have a more representative graphic.
\n\t\t\t
The difference between the simulation and the real time series is due to the measurement method that adds a new noise to the signal, but that noise is not important to the phenomenon. The flow is measured by the difference of pressure in an elbow, so in this method the centrifugal force has to be measured and then the mass flow.
\n\t\t\t
The validation is based in two steps. The first step is the generation of the histogram, autocorrelation graphic and the spectral density graphic (figures 21, 22 and 23) to compare with the results showed in the characterization previous work done by Gavilán (2007). And the second is the evaluation of the phase diagram given by the Hilbert´s transform over the simulated data and the comparison with the real data phase
\n\t\t\t
Figure 21.
Simulated time serie Histogram. (2000 seconds)
\n\t\t\t
Figure 22.
Simulated time serie Autocorrelation factor
\n\t\t\t
Figure 23.
Simulated time serie Spectrogram.
\n\t\t\t
The statistic parameters of the simulated series, (from figures 21, 22 and 23) are coherent with the results found by Gavilán (2007) in the characterization of the bistable flow signal.
\n\t\t\t
To conclude the validation method, is necessary to check if the simulated time series have the same characteristics as the real one in the Hilbert´s sense. So to compare is necessary to repeat the Hilbert´s transform but now to the simulated time series. The results are showed in the figure 24.
\n\t\t\t
In the figure 24, y can be observed the structure of two attractors in the phase, and in the instantaneous frequency. The law for the transitions between one and the other attractor, in the phase space, is an arcos(K(t)) and the instantaneous frequency is the derivate of it. If we compare the figures17, 18, 19, 20 and 24 can be concluded that the simulated series adjust fairly to the real one. The adjustment appears very qualitative but must remember that the relationship in statistical.
\n\t\t\t
Figure 24.
Results of Hilbert´s transform to simulated data.
\n\t\t\t
The characterization of the bistable flow results in, that is non linear and non stationary process. The bistable flow can be evaluated and characterized, properly using the Hilbert´s Transform.
\n\t\t\t
The bistable flow appears under some operative conditions without change in the values of control variables, so is associated to an internal variation. The variation is due to the turbulence that is a self oscillatory process in a continuous medium. The development of a turbulence event is the birth of an attractor in the space of the phase. The turbulence is a convective process because it doesn´t increase indefinitely but rather it evolve to water bellow. All those conclusions about the turbulence justify that the flow change the average value of the speed and then the value of the flow taking one of the two values of the attractor.
\n\t\t\t
The turbulence is an unstructured process, because there´s no pattern in it. The Fourier transform gives poor results when is used so that´s the reason why the turbulence is associated to a noise, and sometimes to a white noise. The phenomenon of bistable flow is a turbulence induced transition process, because the reason of the jumping between two flow values is the turbulence. Because turbulence and noise are physically the same, mathematically the phenomenon can be simulated with a noise-induced transition model.
\n\t\t\t
The simulation gives us, a low error simulated time series that had the same statistical parameters than the original. And the other idea is that the bistable flow only appears in a finite region of the flow space, because there´s only a set of values of the turbulence and flow that induce the bistable behavior. In the same idea, the noise (turbulence) has only one value to induce the bistable flow. The characteristics parameter of the noise is the average that must be zero, and the standard deviation that has a relationship with the value of flow, and only in a small interval of values produce the bistable flow (table 3, figure 25). The relationship between the noise standard deviation and the flow depends in every installation because depends on the hardware but in this case can be modeled by the equation (62).
Relationship between average flow and noise typical deviation.
\n\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Interval Time
\n\t\t\t\t\t\t
Min. Flow (t/h)
\n\t\t\t\t\t\t
Max. Flow(t/h)
\n\t\t\t\t\t\t
Average Error
\n\t\t\t\t\t\t
Typical Deviation
\n\t\t\t\t\t\t
R2 adjusts lineal
\n\t\t\t\t\t\t
Bistability \n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
300-1200
\n\t\t\t\t\t\t
4423.9
\n\t\t\t\t\t\t
5479.2
\n\t\t\t\t\t\t
4.17
\n\t\t\t\t\t\t
32.01
\n\t\t\t\t\t\t
0.9844
\n\t\t\t\t\t\t
No
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
1500-3000
\n\t\t\t\t\t\t
5323.3
\n\t\t\t\t\t\t
5682.9
\n\t\t\t\t\t\t
-41.01
\n\t\t\t\t\t\t
30.47
\n\t\t\t\t\t\t
0.7952
\n\t\t\t\t\t\t
No
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
3001-10000
\n\t\t\t\t\t\t
5492.7
\n\t\t\t\t\t\t
5825.7
\n\t\t\t\t\t\t
0.091
\n\t\t\t\t\t\t
40.71
\n\t\t\t\t\t\t
0.5011
\n\t\t\t\t\t\t
Yes
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
10001-13000
\n\t\t\t\t\t\t
5579.9
\n\t\t\t\t\t\t
5867.7
\n\t\t\t\t\t\t
0.055
\n\t\t\t\t\t\t
36.72
\n\t\t\t\t\t\t
0.2972
\n\t\t\t\t\t\t
Yes, smaller
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
13001-15000
\n\t\t\t\t\t\t
5695.3
\n\t\t\t\t\t\t
5929.5
\n\t\t\t\t\t\t
0.0455
\n\t\t\t\t\t\t
29.63
\n\t\t\t\t\t\t
0.1482
\n\t\t\t\t\t\t
Residual
\n\t\t\t\t\t
\n\t\t\t\t
Table 3.
Parameters of several intervals of flow and conditions.
\n\t\t
\n\t\t
\n\t\t\t
7. Conclusion
\n\t\t\t
This chapter has shown that turbulence exercises several effects in the industrial setting, and except for those of diffusion, or mixture, all the others are both harmful and give rise to problems. Given its variety and applicability, it is not easy to discover the reason for a component failure. Sometimes, only an in-depth analysis of the problem from the perspective of turbulence can serve to clarify a case that defies normal analyses.
\n\t\t\t
Therefore, a thorough grounding in fluid mechanics is needed to solve the problems related to turbulence. Furthermore, a knowledge of computer fluid flow modelling tools, which involves so-called Computational Fluid Dynamics (CFD), represents a great advance in the knowledge of the turbulent behaviour of fluids in movement. Notwithstanding, all is not science and mathematics, given that turbulence is chaos, and therefore, there will always be a certain druidic facet in those professionals given to working in this field.
\n\t\t\t
As an end to this chapter, we provide a summary table (Table 4) that can serve as a guide to locating what effect may be producing the turbulence, with the aim in mind of better focusing the subsequent analyses.
\n\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Fluid type // condition
\n\t\t\t\t\t\t
Turbulence effect
\n\t\t\t\t\t\t
Affected area
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Single-phase // Saturated fluid
\n\t\t\t\t\t\t
Cavitation
\n\t\t\t\t\t\t
Venturi tubes and geometry changes
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Single-phase fluid
\n\t\t\t\t\t\t
Instability
\n\t\t\t\t\t\t
Connection geometry
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Noise // Acoustic Excitation
\n\t\t\t\t\t\t
Connection geometry
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
Fluid structure
\n\t\t\t\t\t\t
Vibration
\n\t\t\t\t\t\t
Elements submerged in the current
\n\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
Noise // Acoustic Excitation
\n\t\t\t\t\t\t
Elements submerged in the current
\n\t\t\t\t\t
\n\t\t\t\t
Table 4.
Summary of fluid type and effects.
\n\t\t\t
Lastly, a look at the use of CFD´s. The use of this type of tool does not ensure the success of the study, given that employing it a preliminary analysis needs to be done and the following defined:
\n\t\t\t
Model type (LES, RANS, DNS…)
Minimum modelling scale (Kolmogorov)
Analysis type (permanent or provisional system)
Variable to be simulated (density, pressure or speed)
\n\t\t\t
Otherwise, the results from the computer will be nothing more that a nicely coloured drawing, without any coherent information.
\n\t\t\t
\n\t\t\t\t“Linearity is an idea sought after, yearned for and forced by the human mind. It is a reflection of our condition as animal. Only by accepting, assimilating and understanding the chaos of the world that surrounds us, will we truly ascend to the rational condition.”
\n\t\t
\n\t\n',keywords:null,chapterPDFUrl:"https://cdn.intechopen.com/pdfs/6732.pdf",chapterXML:"https://mts.intechopen.com/source/xml/6732.xml",downloadPdfUrl:"/chapter/pdf-download/6732",previewPdfUrl:"/chapter/pdf-preview/6732",totalDownloads:9439,totalViews:742,totalCrossrefCites:0,totalDimensionsCites:2,totalAltmetricsMentions:3,impactScore:1,impactScorePercentile:70,impactScoreQuartile:3,hasAltmetrics:1,dateSubmitted:null,dateReviewed:null,datePrePublished:null,datePublished:"January 1st 2010",dateFinished:null,readingETA:"0",abstract:null,reviewType:"peer-reviewed",bibtexUrl:"/chapter/bibtex/6732",risUrl:"/chapter/ris/6732",book:{id:"3194",slug:"computational-fluid-dynamics"},signatures:"Carlos Gavilán Moreno",authors:[{id:"6829",title:"Dr.",name:"Carlos",middleName:null,surname:"Gavilán Moreno",fullName:"Carlos Gavilán Moreno",slug:"carlos-gavilan-moreno",email:"cgavilan@iberdrola.es",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",institution:null}],sections:[{id:"sec_1",title:"1. Introduction",level:"1"},{id:"sec_2",title:"2. Turbulence.",level:"1"},{id:"sec_2_2",title:"2.1. General theory",level:"2"},{id:"sec_3_2",title:"2.2. Kolmogorov´s theory",level:"2"},{id:"sec_4_2",title:"2.3. Simulation of turbulent fluids.",level:"2"},{id:"sec_6",title:"3. General effects of the turbulence. (FIC)",level:"1"},{id:"sec_7",title:"4. Fluid Induced Vibration. (FIV)",level:"1"},{id:"sec_8",title:"5. Fluid Induced Noise (FIN)",level:"1"},{id:"sec_9",title:"6. Fluid induced instabilities.",level:"1"},{id:"sec_10",title:"7. Conclusion",level:"1"}],chapterReferences:[{id:"B1",body:'\n\t\t\t\t\n\t\t\t\t\tUnited Stated Nuclear Regulatory Commision.\n\t\t\t\t\t1986 Anomaluos behavior of recirculation loop flow in jet pump BWR plants, Information. notice 86-110\n\t\t\t\t\t86\n\t\t\t\t\t110 . 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American Institute of Aeroanutics.\n\t\t\t'},{id:"B20",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tVan Herpe\n\t\t\t\t\t\t\tF.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCrighton\n\t\t\t\t\t\t\tD. G.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1994\n\t\t\t\t\tNoise generation by turbulent flow in ducts. Journal de Physique III, 4\n\t\t\t\t\t947\n\t\t\t\t\t950 .\n\t\t\t'},{id:"B21",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tDavies\n\t\t\t\t\t\t\tP.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHolland\n\t\t\t\t\t\t\tK. R.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t2004 Flow Noise generation in expansion chambers. Procc of the Institute of Acoustic.\n\t\t\t\t\t26 Pt. 2, 206\n\t\t\t\t\t213 .\n\t\t\t'},{id:"B22",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tHamakawa\n\t\t\t\t\t\t\tH.\n\t\t\t\t\t\t\n\t\t\t\t\t\tet al.\n\t\t\t\t\t\n\t\t\t\t\t2006 Effects of flow induced Acoustic resonance on vortex shedding form Staggered tube banks. JSME International Journal. Series B, 49\n\t\t\t\t\t1\n\t\t\t\t\t142\n\t\t\t\t\t151 .\n\t\t\t'},{id:"B23",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tChen\n\t\t\t\t\t\t\tS. S.\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tWambsganss Jr\n\t\t\t\t\t\t\tM. W.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1970\n\t\t\t\t\tResponse of a flexible rod to near field flow noise.\n\t\t\t\t\tProceedings of the conference on flow induced vibrations in reactor system components. ANL-7685 Argonne National Laboratory.\n\t\t\t'},{id:"B24",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tCorcos\n\t\t\t\t\t\t\tG. M.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1963\n\t\t\t\t\tResolution of pressure in turbulence\n\t\t\t\t\t. J. Acoustic Soc. Am. 35\n\t\t\t\t\t2\n\t\t\t\t\t192\n\t\t\t\t\t199 .\n\t\t\t'},{id:"B25",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tPaïdoussis\n\t\t\t\t\t\t\tM. P.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1973\n\t\t\t\t\tDynamics of cylindrical structures subjected to axial flow. Journal of Sound and vibration. 29\n\t\t\t\t\t3\n\t\t\t\t\t365\n\t\t\t\t\t385 .\n\t\t\t'},{id:"B26",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tSaito\n\t\t\t\t\t\t\tN.\n\t\t\t\t\t\t\n\t\t\t\t\t\tet al.\n\t\t\t\t\t\n\t\t\t\t\t2002 BWR 9x9 Type Rod Assembly Thermal Hydraulic Test (2)- Hydraulic Vibration Test. 10th International Conf. on Nuclear Engineering. ICONE10\n\t\t\t\t\t22557 .\n\t\t\t'},{id:"B27",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLighthill\n\t\t\t\t\t\t\tM. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1952\n\t\t\t\t\tOn Sound Generated Aerodynamically. I. General Theory, Proc. R. Soc. Lond. A 211 564\n\t\t\t\t\t587 .\n\t\t\t'},{id:"B28",body:'\n\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\tLighthill\n\t\t\t\t\t\t\tM. J.\n\t\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t1954\n\t\t\t\t\tOn Sound Generated Aerodynamically. II. Turbulence as a Source of Sound\n\t\t\t\t\t, Proc. R. Soc. Lond. A 222 1\n\t\t\t\t\t32 .\n\t\t\t'}],footnotes:[{id:"fn1",explanation:"These are referred to as coherent because the vorticity is concentrated and the fluid flows around as if it were a solid obstacle. It keeps its shape for longer than a single rotation."}],contributors:[{corresp:"yes",contributorFullName:"Carlos Gavilán Moreno",address:"",affiliation:'
Cofrentes N.P.P. Iberdrola S.A., Spain
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1. Introduction
With the increasing demand for herbal medicinal products, nutraceuticals, and natural products for primary healthcare worldwide, medicinal plant extract manufacturers and essential oil producers have started using the most appropriate extraction techniques. Different methods are used to produce extracts and essential oil of defined quality with the least variations.
Herbs and medicinal plants have been used for centuries as source of a wide variety of biologically active compounds. The plant crude material or its pure compounds are extensively used to treat diverse ailments by generations of indigenous practitioners [1, 2]. They are currently the subject of much research interest, but their extraction as part of phytochemical and biological investigations presents specific challenges that must be addressed throughout the solvent extraction [3]. Natural products provide unlimited opportunities for new drug discovery because of the unmatched availability of chemical diversity [4]. Thanks to two drugs derived from alkaloids of Madagascar’s rosy periwinkle (Catharanthus roseus), the likelihood of remission for a child who has leukemia increased by 85 percent between 1960 and 1997 [5, 6]. New compounds, such as one recently discovered in a plant in Madagascar, are likely to provide novel antibiotics and help to curb the epidemic of antibiotic-resistant diseases [7].
Natural products are currently of considerable significance due to their unique attributes as a significant source of therapeutic phytochemicals and their efficacy, safety, and minimal side effects [2, 8]. Bioactive compounds in plants include alkaloids, terpenoids, coumarins, flavonoids, nitrogen-containing compounds, organosulfur compounds, phenolics, etc. A wide spectrum of bioactivities is exhibited by these compounds such as anti-inflammatory, immunostimulatory, anticancer, antioxidant, antimicrobial, etc.
Research on medicinal plants is particularly important as that on conventional drugs due to the beneficial phytochemicals from plants and the shift towards natural products in pharmaceutical and cosmeceutical industries. Chemical structures of a few essential bioactive compounds isolated from plants are presented in Figure 1 [9, 10, 11, 12, 13, 14].
Figure 1.
Chemical structures of a few important bioactive compounds isolated from plants.
Extraction of the bioactive constituents from plants has always been challenging for researchers [15]. As the target compounds may be non-polar to polar and thermally labile, the suitability of the extraction methods must be considered. The study on medicinal plants starts with extraction procedures that play a critical role in the extraction outcomes and the consequent assays.
Hence, this chapter aims to provide an overview of the process of plant extraction, describe, and compare extraction methods based on their principle, the effect of solvent on extraction procedures, strength, limitations, and economic feasibility, with their advantages and disadvantages. This chapter shall also emphasize the common problems encountered and methods for reducing or eliminating these problems. Since millions of natural products derived from plants are known, only selected groups and compounds are presented.
2. Medicinal plants and herbs
The term “medicinal” as applied to a plant indicates that it contains a substance or substances which modulate beneficially the physiology of sick mammals, and man has used it for healthful purpose [16]. Medicinal plants were described by Farnsworth and Soejarto as: “all higher plants with medicinal effects that relate to health, or which are proven as drugs by Western standards, or which contain constituents that are defined as hits.” [17].
Medicinal plant (MP) refers to any plant which, in one or more of its organs, contains substances that can be used for therapeutic purposes or which are precursors of the synthesis of valuable drugs. A whole plant or plant parts may be medicinally active [18, 19, 20, 21, 22]. Medicinal plants (MPs) are becoming very important due to their uses mainly as a source of therapeutic compounds that may lead to novel drugs. MPs are plants that are used for healthcare purposes in both allopathic and traditional medicine systems. MPs cover various species used including condiments, food aromatic and cosmetics [23, 24, 25, 26].
Herbs may be defined as the dried leaves of aromatic plants used to impart flavor and odor to foods with, sometimes, the addition of color. The leaves are commonly traded separately from the plant stems and leaf stalks [27].
Herbal medicine is referred to as medicinal preparations comprising active ingredients obtained from the herbal plant. The product can be made from the whole plant or any part. Preparations from by-product herbal plants such as oil, gum, and other secretions are also considered herbal medicines [18, 19, 22].
3. Primary and secondary metabolites
Metabolites are intermediate processes in nature and are small molecules. Primary metabolites are known vital or essential compounds and are directly involved in the average growth, development, and reproduction of plants [28]. Primary metabolites include cell constituents (e.g. carbohydrates, polysaccharides, amino acids, sugars, proteins, and lipids) and fermentation products (ethanol, acetic acid, citric acid, and lactic acid), and are mainly used during their growth and development stages [19, 22, 29, 30].
Secondary metabolites are not directly involved in those processes and usually have a function but are not that important for the organism (e.g. phenolic, steroids, lignans, etc.). They are found only in specific organisms or groups of organisms, and express of the individuality of species [19, 30, 31]. They are not necessarily produced under all conditions, and most often, the function of these compounds and their benefit to the organism is not yet known. Some are undoubtedly made for readily appreciated reasons, e.g., as toxic material providing defense against predators, as volatile attractants towards the same or other species, but it is logical to assume that all do not play some vital role for the well-being of the producer [27, 30]. Secondary metabolites are produced after the growing stage and are used to increase the ability of plants to survive and overcome their local challenges. Bioactive compounds are classified as terpenoids, alkaloids, nitrogen-containing compounds, organosulfur compounds, and phenolic compounds [29].
Bioactive compounds are reported to possess diverse bioactivities such as antioxidant, anticancer, antimalarial, antiulcer, antimicrobial, anti-inflammatory activity [32, 33, 34, 35, 36].
4. Bioactive compounds
The definition of bioactive compounds remained ambiguous and unclear for a long time. Very few references describe the term “bioactive”. It is composed of two words bio- and -active. In etymology bio- is from the Greek (βίo-) “bios” that means life while -active is derived from the Latin word “activus” that refers to dynamic, full of energy, with energy, or involved in activity [37, 38, 39]. The term “bioactive” is an alternative term for “biologically active” [40]. Hence, a bioactive compound is simply a substance with biological activity [41, 42].
A plant extract is a substance or an active substance with desirable properties removed from the tissues of a plant, frequently by treating it with a solvent, to be used for a particular purpose. The term “bioactive compounds” is generally referred to as biologically significant chemicals but not established as essential nutrients [43]. Bioactive compounds are essential (e.g., vitamins) and non-essential (e.g., polyphenols, alkaloids, etc.) compounds that occur in nature, are part of the food chain, and can affect human health [44]. They are derived from various natural sources such as plants, animals, microorganisms (e.g., fungi) and marine organisms (e.g., lichens) [2]. The amount of bioactive natural products in natural sources is always fairly low [45, 46]. Plant active compounds are usually contained inside plant matrixes. Active compounds are synthesized in small quantities and different concentrations in all plant organs or parts such as leaves, roots, barks, tubers, woods, gums or oleoresin exudations, fruits, figs, flowers, rhizomes, berries, twigs, as well as the whole plant. Further processes may be required after extraction to purify or isolate the desired compounds.
5. Fresh or dried plant materials
Fresh and dried samples are used and are reported in the literature in the preparation of medicinal remedies. Ideally, fresh plant tissues should be used for phytochemical analysis, and the material should be plunged into boiling alcohol within minutes of its collection. Alternatively, plants may be dried before extraction [47]. In most reported cases, dried materials are preferred considering their long conservation time compared to fresh samples. Furthermore, fresh specimens are fragile and tend to deteriorate faster than dried ones. Phytoconstituents such as Essential Oils (EOs) are found in fewer dried samples than in fresh samples. In case of fresh plant material extraction using organic solvents such as methanol or ethanol, is required to deactivate enzymes present in the plant sample. The extractive might contain a substantial portion of water; hence it can be partitioned using specific immiscible organic solvents [3].
6. Drying procedures
Drying is the most common method to preserve the plant material from enzymatic degradation, such as hydrolysis of glucoside, etc. It should be dried as quickly as possible in the open room under primitive conditions at ambient room temperature with air circulation around the plant material to avoid heat and moisture [47]. However, they placed in shallow trays with good atmospheric air-up dryness either in the sunshine or in shade depending on nature of the indicated or identified constituents. However, direct sunlight is usually avoided to reduce the possibility of chemical reactions, responsible for forming of the artifact that may result from chemical transformations after exposure to ultraviolet radiation. Alternatively, plant materials should be dried under optimum temperature conditions between 40 and 50°C, or they can be dried in the oven if needed. Generally, plant material is dried at temperatures below 30°C to avoid the decomposition of thermolabile compounds [3]. Plants containing volatile or thermolabile components may be lyophilized (freeze-dried). In freeze-drying the frozen material is placed in an evacuated apparatus with a cold surface maintained at −60 to −80°C. Water vapors from the frozen material then pass rapidly to the cold surface to yield the dry material [8, 48].
7. Grinding or powdering plant materials
Lowering particle sizes increase surface contact between samples and extraction solvents and therefore, increase the yield rate and yield. Grinding resulted in coarse smaller samples, meanwhile, powdered samples gave a more homogenized and smaller particle, leading to better surface contact with solvents used for extraction. Before the extraction, pretreatments such as drying and grinding of plant materials are usually conducted to increase the extraction efficiency [48]. It is essential that the particles are of as uniform size as possible because larger particles take a longer time to complete the extraction process [49]. Usually, solvent molecules most contact the larger analytes, and particle size smaller than 05 mm is ideal for efficient extraction [8]. Conventional methods are usually used to reduce the particle size of dried plant samples viz. mortar and pestle or electric blenders and mills, etc.
8. Extraction techniques of actives compounds from plants and herbs
Extraction is separating the medicinally active mixture of many naturally active compounds usually contained inside plant materials (tissues) using selective solvents through the standard procedure [50]. It can also be defined as the treatment of the plant material with solvent, whereby the medicinally active constituents are dissolved and most of the inert matter remains undissolved. Thus, the purpose of all extraction is to separate the soluble plant metabolites, leaving behind the insoluble cellular marc known as residue [8]. The obtained product is a relatively complex mixture of metabolites, in liquid or semisolid state or (after removing water) in dried powder form, and are intended for oral and/or external uses. Extraction is based on the difference in solubility between the solute, other compounds in the matrix, and the solvent used to stabilize [29].
In general, there are three common type of extractions: liquid/solid, liquid/liquid and acid/base [51]. The extraction of these active compounds needs appropriate extraction methods that consider the plant parts used as starting material, the solvent used, extraction time, particle size and the stirring during extraction [52, 53]. Extraction methods include solvent extraction, distillation method, pressing, and sublimation according to the extraction principle. Solvent extraction is the most widely used method [47].
The solvent used, the plant part used as starting material and the extraction procedure are three basic parameters reported that influence the quality of an extract [15]. Proper extraction procure is the first step towards isolating and identifying the specific compounds in crude herbal material. It plays a significant and crucial role in the outcome. Successful extraction begins with careful selection and preparation of plant sample and thorough review of the appropriate literature for indications of which protocols are suitable for a particular class of compounds or plant species [3]. For instance, if the components are volatile or prone to degradation, they can first be frozen and homogenized with liquid nitrogen [29]. The extraction, in most cases, involves soaking the plant material in solvent for some specific time. Reported properties on an excellent extraction solvent include low toxicity, preservative action, ease of evaporation at low heat, promotion of rapid physiologic absorption of the extract, and inability to cause the extract to be complex or dissociate.
The principle of solid–liquid extraction is that when a solid material comes in contact with the solvent, the soluble components in the solid material are dissolved in, and move to the solvent. In solvent extraction, the mass transfer of soluble ingredients to the solvent takes place in a concentration gradient. The mass transfer rate depends on the concentration of ingredients, until equilibrium is reached. After that, there will no longer be a mass transfer from plant material to the solvent. In addition, heating the solvent can also enhance the mass transfer because of better solubility.
Moreover, the concentration gradient changes if fresh solvent replace the solvent equilibrium with the plant material [50]. Properties required for an excellent extracting solvent (or a mixture of solvents) include removal, inert, non-toxic, free from plasticizers, not easily inflammable, and no or less chemical interaction [53]. The selection of solvent is therefore crucial for solvent extraction. Solubility, selectivity, cost, and safety should be taken into account in selecting solvent [47]. The factors affecting the choice of solvent are quality of phytochemicals to be extracted, rate of extraction, diversity of metabolites extracted, the toxicity of the solvent in the bioassay process, and the potential health hazard of the extractants and ease of subsequent handling of the extract. Obtaining maximum yield and the highest quality of the targeted compounds is the central goal of the extraction process [29]. Extraction methods are usually chosen per the properties of targeted active compounds, the water content of the plant material, and the objectives of extraction. Initially, natural bioactive compounds are extracted using various extraction techniques, and their bioactivities are identified using in vitro and in vivo testing [45, 47]. A successful determination of biologically active compounds from plant material is largely dependent on the type of solvent used in the extraction. Since the extract will contain traces of residual solvent, the solvent should not interfere with the bioassay [15].
Various conventional (classical) and non-conventional (innovative) methods can extract plant materials. Variation in extraction procedures usually depends on key factors as extraction time, the temperature used, the particle size of tissues, the solvent-to-sample ratio, the pH of the solvent.
8.1 Classical and/or conventional techniques
The commonly employed extraction methods (long been used) are primarily based on liquid–solid extraction. They are ordinarily easy to operate and are based on heat and/or solvents with different polarities.
8.1.1 Maceration
This process is conducted by soaking the plant materials (coarse or powered) in a closed stoppered container in a solvent allowed to stand at room temperature for 2–3 days with frequent stirring to obtain plant extracts. A sealed extractor is used to avoid solvent evaporation at atmospheric pressure. The process is intended to soften and break the plant’s cell walls to release the soluble phytoconstituents. The mixture is then pressed or strained by filtration or decantation after a specific time [8, 54]. Maceration is the simplest and still widely used procedure. The extraction procedure in this stationary process works on principle of molecular diffusion, which is a time-consuming process. Maceration ensures dispersal of the concentrated solution accumulation around the particles’ surface and brings fresh solvent to the surface of particles for further extraction [46].
8.1.2 Digestion
This is a kind of maceration in which gentle heat is applied during the maceration extraction process. The temperature does not alter the active ingredients of plant material, so there is greater efficiency in the use of menstruum (solvent or mixture of solvent used for extraction). It is used when the moderately elevated temperature is not objectionable and the solvent efficiency of the menstruum is increased thereby [15]. The most used temperatures are between 35 and 40°C, although it can rise to no higher than 50°C. The plant part to be extracted is placed in a container with the pre-heated liquid to the indicated temperatures, is maintained for a period that may vary between half an hour to 24 hours, shaking the container regularly. This process is used for the herbal material or plant parts that contain poorly soluble substances or polyphenolic compounds [49].
8.1.3 Infusion
Infusion is a simple chemical process used to extract plant material that is volatile and dissolves readily or release its active ingredients easily in organic solvents [49]. Infusion and decoction use the same principle as maceration; both involve soaking the plant material in boiled or cold water which is then allowed to steep in the liquid. The maceration time for infusion is, however shorter. The liquid may then be separated and concentrated under a vacuum using a rotary evaporator.
Infusion finds its application in tea preparation and consumption prescribed in psychophysical asthenia, diarrhea, bronchitis, asthma, etc. In Tropical Africa, the infusion of the bark of Prunus africana (pygeum) is taken orally to increase the ease of urination and reduce inflammation and cholesterol deposits [30].
8.1.4 Lixiviation (elution)
The word “lixiviation” (comes from the Latin lixivium, “lessive”.) The extraction is carried out with cold or boiled, fresh and new solvent, always. Extraction of components is done using water as solvent.
8.1.5 Decoction
The current process involves boiling the plant material in water to obtain plant extracts. Heat is transferred through convection and conduction, and the choice of solvents will determine the type of compound extracted from the plant material [8]. The sample is boiled in a specified volume of water for a defined time (15 to 60 minutes.) It is then cooled, strained, filtered, and added enough water through the drug to obtain the desired volume. This method is suitable for extracting thermostable (that does not modify with temperature) and water soluble compounds, hard plant materials and commonly resulted in more oil-soluble compounds than maceration.
8.1.6 Tincture
It is the extraction of plant material in alcohol. Usually, the plant material (fresh) and ethyl alcohol are taken at the ratio of 1:5. Because of the alcohol content, the tinctures can be stored at room temperatures without decomposing [55].
8.1.7 Percolation
It is conducted by passing the boiled solvent through the plant material at a controlled and moderate rate (e.g. 5–7 drops per min) until the extraction is complete before evaporation. The concentrated plant extracts are commonly collected at the bottom of the vessel. To obtain a significant amount of extract, successive percolations can be performed by refilling the percolator with fresh solvent and pooling all extracts together. This procedure is mostly used to extract active compounds in the preparation of tinctures and fluid extracts. Its major disadvantage is that large volumes of solvents are required, and the procedure can be time-consuming and may require skilled persons [49].
8.1.8 Steam distillation and hydrodistillation
Steam and hydrodistillation methods are usually used to extract volatile compounds, including essential oil, insoluble in water, from various aromatic and medicinal plants. This is conducted by boiling the plant materials in water to obtain EOs after vapor condensation. Steam distillation occurs at a temperature lower than the boiling point of the ingredients. The method is useful for thermos-sensitive bioactive compounds e.g., natural aromatic compounds. The heat leads to breakage in the sample’s pores and then enables the release of the target compound from a matrix. As Raoult’s law states that while mixing two immiscible liquids, the boiling point will be reduced. Therefore, in the mixture of volatile compounds having a boiling point between 150 and 300°C and water having a boiling point at about 100°C (at atmospheric pressure), the mixture evaporation will be getting closer to that of the water [29, 56].
There are similarities between the hydrodistillation and the steam distillation principles. In brief, plant material is immersed in water or a proper solvent followed by heating to boiling under atmospheric pressure in the alembic. In a condenser, EOs vapors and water undergo a liquefaction process, and EOS are then separates from water/solvent after collection of the condensate in the decanter. The principle of extraction is based on isotropic distillation. Hydrodistillation with water immersion, direct vapor injection, and water immersion and vapor injection are the three main types of hydrodistillation. The distillation time depends on the plant material being processed [56].
8.1.9 Hot continuous extraction or Sohxlet extraction, soxhletation
In this method, finely ground sample is placed in a porous bag or “thimble” made from a strong filter paper or cellulose, set in the thimble chamber of the Soxhlet apparatus. The first Soxhlet apparatus was developed in 1879 by Franz von Soxhlet (Figure 2) [58]. Extraction solvents are heated in a round bottom flask, vaporized into the sample thimble, condensed in the condenser, and dripped back. When the liquid content reaches the siphon arm, the liquid content is emptied into the bottom flask again, and the process is continued [8]. The disadvantages include no possibility of stirring, and a large amount of solvent is required. This method is unsuitable for thermolabile compounds as prolonged exposure (long extraction time) to heat may lead to their degradation. It constitutes an official classical method used to determine different foods’ fat content [15, 29, 57].
Figure 2.
Experimental Soxhlet extraction apparatus [57].
Exposure to hazardous and flammable liquid organic solvents are the most noticed disadvantages in this method, and the high purity of extraction solvents needed may add to the cost. Also, shaking or stirring cannot be provided in the Soxhlet device to accelerate the process [57].
However, it requires a smaller quantity of solvent as compared to maceration. Besides, instead of many portions of warm solvent passing through the sample, just one batch of solvent is recycled. Other advantages of this technique include its simple operational mode, its applicability to a higher temperature that increases the kinetics process, its low capital cost, the absence of filtration, and the continuous contact of the solvent and the sample. It maintains a relatively high extraction temperature with heat from the distillation flask [29, 57, 59].
8.1.10 Serial exhaustive extraction
It is a standard extraction procedure that involves successive extraction with various solvents of increasing polarity from non-polar to polar ones. The aim is to ensure that a broad polarity range of compounds could be extracted [15].
Some medicinal preparations adopt the technique of fermentation for extracting the active principles. The extraction procedure involves soaking the crude drug, either a powder or a decoction, for a specified period. Alcohol is generating in situ after fermentation occur; this eases the extraction of the active components contained within the plant material. The alcohol hence generated additionally serves as preservative. Water should be boiled first, if the fermentation is to be performed in an earthen vessel. Wooden vats, porcelain jars, or metal vessels are used in place of earthen vessels in large-scale manufacturing. This method is not yet standardized [50].
Hydrodistillation and steam distillation, hydrolytic maceration followed by distillation, expression and effleurage (cold fat extraction) may be employed for aromatic plants. Some of the latest extraction methods for aromatic plants include headspace trapping, solid phase micro extraction, protoplast extraction, micro distillation [15].
These techniques are the easiest and simplest methods. Despite the establishment of advanced extraction methods, the potential of conventional solid–liquid extractions is still being used to obtain active compounds from plants. These methods are criticized due to large solvent consumption and long extraction times that can destroy some metabolites. Solvents used in these techniques for soaking play a critical role. Many other advanced extraction methods that incorporate various technologies have been developed [8, 48].
8.2 Innovative (non-conventional) techniques
There is steady progress in the development of extraction technology in recent years. They are also known as advanced techniques with the most recently developed.
8.2.1 Microwave-assisted extraction (MAE)
Microwaves are part of the electromagnetic spectrum of light with a range of 300 MHz to 300 GHz, and wavelengths of these waves range from 1 cm−1 to 1 m−1 [60]. These waves are made up of two perpendicular oscillating fields which are used as energy and information carriers.
In this extraction process, the use of microwave energy results in faster heating. Due to the exposure of each molecule to the microwave field, its direct effects include, thermal gradients reduction, volume generation due to heat, equipment size reduction, because of the higher process rates, and thus increase in productivity, through better usage of the same equipment process volume [61]. MAE is a feasible green solvent extraction procedure as it uses water or alcohol at elevated temperature and controlled pressure conditions (Figure 3).
Figure 3.
Schematic representation of microwave-assisted extraction equipment [62].
This procedure has demonstrated various benefits like ease to handle and understand steadiness. Many studies reported that MAE has higher yields and is significantly faster than conventional methods for extracting active substances from plant materials [48, 54, 62]. MAE can be presented as a potential alternative to the traditional soli-liquid extraction techniques. A few of the potential advantages are as follow:
a lesser amount of solvent is required (few milliliters of solvent can be used);
shorter extraction time, from few seconds to few minutes (15–20 min);
improved extraction yield;
favorable for thermolabile constituents;
heavy metals and pesticides residue which is present in the trace can be extracted from a few milligrams of plant sample;
during extraction, it provides a stirring, by which the mass transfer phenomenon is improved [54, 60, 62, 63].
MAE intensification needs special equipment to be functional, and electricity produces waves, leading to higher investments and higher operating costs than conventional methods [64]. Banar and collaborators extracted the bioactive compounds from Urtica dioica grown in Lebanon using conventional methods (maceration, reflux, Soxhlet, hydrodistillation, Ultrasound-Assisted Extraction (UAE) and Microwave-Assisted Extraction (MAE)) with different solvents. Their results revealed that MAE was the most effective technique. The extraction time was reduced, the lesser solvent was used and the amount of extracted compounds was increased [65].
8.2.2 Ultrasound-assisted extraction (UAE) or sonication extraction
This extraction method involves using ultrasound with frequencies ranging from 20 to 2000 KHz; this increases the permeability of cell walls and produce cavitation. Although the process is helpful in some cases, its large-scale application is limited due to its high cost. The most noticeable disadvantage of the procedure is the occasional but known deleterious effect of ultrasound energy on the active components of the medicinal plants through the formation of free radicals and consequently undesirable changes on the drug molecules [50]. The schematic representation of the equipment is given below (Figure 4).
Figure 4.
Schematic representation of an ultrasound-assisted extraction equipment.
Factors that affect the efficiency of UAE are extraction time, power, solvent, Liquid/Solid (L/S) ratio, plant material, frequency, amplitude, and intensity. UAE more advantageous than other advanced extraction methods and provided the best mass and heat transfer efficiency, lowest energy consumption and carbon emission. It was reported to yield high total phenolic content, antioxidant activity, or specific active compounds [62, 66].
8.2.3 Pressurized liquid extraction (PLE) or accelerated solvent extraction (ASE)
Pressurized liquid extraction (PLE) also known as pressurized fluid extraction (PFE), accelerated solvent extraction (ASE), and pressurized solvent extraction (PSE), or as enhanced solvent extraction system (ESE) [67].
Dionex Corporation introduced PLE in 1995 as an alternative to maceration, percolation, sonication, Soxhlet extraction, etc. It is an automated technique for extracting solid samples with liquid solvents (either aqueous or organic, single or mixtures) above their boiling point, combine high pressures (4–12 MPa) and moderate to high temperatures (50–300°C) [68]. When water is the extraction solvent, different terms are used to define the method, that includes hot water extraction (HWE), subcritical water extraction (SWE), high-temperature water extraction (HTWE), hot water extract pressurized (PHWE), liquid water extraction or superheated water extraction [67]. Sample size, solvent, pressure, temperature, pH, flow rate, extraction time are the standard parameters influencing the PLE process, with temperature and solvent type being the most significant ones [69, 70, 71].
In this process, for a short period of time (5–10 min), a cartridge in which the ample has been placed is filled with an extracting solvent and used to statically extract the sample under elevated temperature and pressure. To purge the sample extract from the extraction cell into a collector flask pressurized gas is used (Figure 5) [68].
Figure 5.
Scheme of pressurized liquid extraction equipment [68].
To increase the efficiency of this extraction process, environmentally friendly liquid solvents are used at moderate to elevated temperature and pressure [72]. The increased temperature causes dramatic changes in the physical–chemical properties of water, enhances the analytes’ solubility, breaks matrix-analyte interactions achieving a higher diffusion rate, and accelerates the extraction process by increasing the diffusivity of the solvent. The increased pressure in contrast, keeps the solvent in a liquid state without boiling and forces the solvent to penetrate the matrix pores [55, 73, 74, 75].
The main advantages of this technique are: (i) faster extraction from 15 to 50 min, (ii) low quantity of solvents (15–40 mL), and no filtration is required. However, costly equipment and the need for a throughout optimization of variables to avoid a matrix-dependent efficiency are the main demerits [72, 73, 74].
8.2.4 Supercritical fluid extraction (SFE)
SFE is used for separating components from the matrix with the application of supercritical fluids as the extracting solvent (Figure 6) [30].
Figure 6.
Schematic diagram of supercritical fluid extraction (SFE) set-up [76].
Using CO2 as the extracting fluid has many advantages. Besides, its lower boiling point (31°C) and its critical pressure (74 bar). Moreover, carbon dioxide is abundant in nature, safe and inexpensive. But while carbon dioxide is the preferred fluid for SFE, it possesses several polarity limitations. When extracting polar solutes and when strong analyte-matrix interactions are present solvent polarity is crucial. Carbon dioxide fluid is usually mixed with organic solvents to alleviate the polarity limitations (Figure 7) [2].
Figure 7.
Schematic representation of a supercritical fluid extraction (SFE) system [62].
The SFE extraction procedure possesses distinct advantages:
the extraction of constituents is carried out at a low temperature, strictly avoiding damage from heat and some organic solvents. SFE offers gentle treatment for heat-sensitive material;
fragrances and aroma remain unchanged;
CO2 is an inexpensive solvent;
No solvent residues are left behind;
possibility of direct coupling with analytical chromatographic techniques such as gas chromatography (GC) or supercritical fluid chromatography (SFC);
environmentally friendly extraction procedure. CO2 as the solvent does not cause environmental problems and is physiologically harmless, germicidal, and non-flammable.
Some specific disadvantages of this method are:
high investment cost;
the use of high pressures leads to capital costs for the plant, and operating costs may also be high, so the number of commercial processes utilizing supercritical fluid extraction is relatively small, due mainly to the existence of more economical methods;
high polar substances (sugars, amino acids, inorganic salts, proteins, etc.) are soluble;
phase equilibrium of the solvent/solute system is complex and making design of extraction conditions is difficult.
SFE finds extensive application in extracting pesticides, environmental samples, foods and fragrances, essential oils, polymers, and natural products [50, 77]. Conde-Hernández and collaborators extracted the essential oil of rosemary (Rosmarinus officinalis) by S-CO2 extraction, hydro distillation and steam distillation. They found that both yields of essential oil and antioxidant activity of SFC extract were higher than those from the other two methods [78, 79].
8.2.5 Pulsed electric field (PEF) extraction
Pulsed electric field extraction is a technique based on the exposure of vegetable matrix to an electrical potential. A transformer generates an electric pulse, increasing voltages from 140 or 220 V to 1000 V, or even greater than that (25000 V). A capacitor transforms this high voltage in a closed chamber with metallic electrodes. The general scheme of PEF equipment is presented in Figure 8 [80].
Figure 8.
General scheme of a PEF equipment process.
This “cold” extraction assisted by PEF prevent the degradation of the cell and the extraction of components from the intracellular vacuoles [81]. It considerably increases the yield and decreases the time because it can increase mass transfer by destroying membrane structures during the extraction process.
Specific energy input, treatment temperature and field strength are considered among parameters that can influence the treatment efficacy of the PEF extraction. It is known as a non-thermal method which reduces the decomposition of the thermolabile components [47].
8.2.6 Enzyme-assisted extraction (EAE)
The EAE is an enzymatic pre-treatment that is carried out by the addition of specific hydrolyzing enzymes during the extraction step. In the cell membrane and cell wall structure, micelles are formed by macromolecules such as polysaccharides and protein. The coagulation and denaturation of proteins at high temperatures during extraction are the main barriers to extracting natural products. EAE enhance the extraction efficiency due to the hydrolytic action of the enzymes on the components of the cell wall and membrane and the macromolecules inside the cell, which facilitate the release of the natural products. Cellulose, α-amylase, and pectinase are hydrolyzing enzymes usually employed in EAE [47, 82]. This procedure is suitable for extracting various bioactive substances from plant matrices, but after filtration the obtained fraction is rich in small water-soluble molecules that include polyphenols and flavonoids [82].
8.2.7 Turbo-distillation extraction or turbo-extraction (turbolysis)
Turbo-distillation was patented in 1983 by Martel, and has been used in several companies as an industrial purpose for extracting EOs from hard matrixes (such as wood, bark, and seeds) [83]. The extraction process is similar to hydrodistillation with slight modifications [84]. The turbo-extraction or turbolysis is based on extraction with stirring and simultaneous reduction of particle size. Due to of high shearing force, cells disruption leads to rapid dissolution of the active constituents. It results in an extraction time of the order of minutes and the plant content is almost completely depleted [85]. Compare to hydrodistillation, turbo-distillation minimize extraction time and energy consumption and prevents the degradation of volatile constituents (Figure 9) [84].
Figure 9.
Laboratory turbo-Clevenger: (a) schematic, (b) bench apparatus. The vessel (1); the rotor (2); the turbo shredder (3); the thermometer (4); the distillation column (5); the condenser (6); the receiver-cum separator (7) [46, 84].
In 2017, Martins and collaborators studied the turbo-extraction of stevioside and rebaudosideo A from Stevia rebaudiana dried and powdered leaves. The extraction is carried out by applying a fractional factorial design that allowed the evaluation of the main effects of drug powder size, solvent to drug ratio by weight, temperature, stirring and time on the yield of these glycosides. Their work demonstrated that turbo-extraction was promising for Stevia rebaudiana glycosides extraction. It stimulated new research on the purification of these extracts, which became an exciting source of income for developing countries such as India and Brazil [86]. Perino and collaborators showed that the essential oil extracted by turbodistillation in 30 minutes were quantitatively (yield and kinetics profile) and qualitatively (aromatic profile) similar to those obtained using conventional hydrodistillation in 3 hours. They concluded that this process, which gave a reduced extraction time, was perfectly adapted to the extraction of hard matrixes [84]. It can be advantageous over dynamic maceration.
8.2.8 Counter-current extraction (CCE)
In this procedure, the wet raw material is pulverized to produce a fine slurry. The target material is moved in one direction (usually as a fine slurry) within a cylindrical extractor where it comes in contact with extracting solvent. Further, the starting material moves making more concentrated extract. Thus, complete extraction is possible when the amounts of material and the flow rate of solvent are optimized the complete extraction is possible. The process is extremely efficient, takes little time and poses no danger when high temperature is applied. Lastly, the extracts come out sufficiently concentrated at one end of the extractor, while the residue falls on the other end [50]. This extraction procedure has great advantages:
compared to other methods such as maceration, decoction, percolation a unit amount of the plant material cab be extracted with a much smaller volume of solvent;
CCE is usually performed at room temperature, which avoids the thermolabile constituents from being exposed to heat which is used in most other techniques;
Since the drug is pulverized under wet conditions, the heat generated during comminution is neutralized by water. This once more avoids the thermal degradation of components from heat exposure;
Compare to continuous hot extraction, CCE is rated to be more efficient and effective.
8.2.9 Solid-phase extraction (SPE)
Solid-phase extraction (SPE) is a sample preparation technology using chromatographic packing material, solid particle, commonly found in a cartridge-type device, to chemically separate the different components. Samples are almost constantly in the liquid state (although special applications can be run with some samples in the gas phase). In this method, the dissolved or suspended compounds in a liquid mixture are separated from other compounds depending on their physical and chemical properties. The technically correct name for this technology is “Liquid–Solid Phase Extraction”, since the chromatographic particles are solid and the sample is in the liquid state [87].
SPE has many benefits, but four significant benefits deserve special attention:
simplification of complex sample matrix along with compound purification;
reduce ion suppression or enhancement in MS applications;
capability to fractionate sample matrix to analyze compounds by class;
trace concentration (enrichment) of very low-level compounds.
This rapid, economical and sensitive technique uses different types of cartridges and disks, with various sorbents, where the solute molecules are preferentially attached over the stationary phase.
8.2.10 High-voltage-assisted extraction
The principle of this equipment is similar to PEF, with the difference that electrical discharge is made through a small point. For this, a needle electrode is used from which the release is made in a plate ground electrode.
These methods are known as greener methods, are often better than conventional ones in terms of high yields, high selectivity, lower solvent consumption and shorter extraction time. They are also found to be environmentally ecofriendly since energy, and organic solvent consumption are reduced. The combination of extraction methods to obtain high purity extracts or high overall yields are described in the literature [40, 88, 89, 90]. Its main advantage is the operability in continuous mode, which is very important from an industrial and economic point of view [80].
8.2.11 Phytonics process
A new solvent-based on hydrofluorocarbon-134a and a new technology to optimize its remarkable properties in the extraction of plant material offer significant environmental advantages and health and safety benefits over traditional processes to produce advanced quality natural fragrant oil, flavors and biological extracts.
The technology known as “phytonics process” was developed and patented by Advanced Phytonics Limited (Manchester, UK). Fragrant components of EOs and biological or phytopharmacological extracts that can be used straightly without additional chemical or physical treatment are the products frequently extracted by this process. The properties of the new generation of fluorocarbon solvents have been applied to the extraction of plant material. The core of the solvent is 1,1,2,2-tetrafluoroethane, better known as hydrofluorocarbon-134a (HFC-134a) with a boiling point of – 25°C; a vapor pressure of 5.6 bar at ambient temperature. It is flammable and non-toxic. This product was developed as a replacement for chlorofluorocarbons and more importantly, it does not deplete the ozone layer. By most standards this is a poor solvent that is unable to break up (dissolve) plant waste.
The process is advantageous because the solvents can be customized: by using modified solvents with HFC-134a, the process can be made highly selective in extracting a specific class of phytoconstituents. Likewise, to withdraw a broader spectrum of constituents other modified solvents can be employed. The biological products obtained by this process contain extremely low residual solvent. Residuals are constantly below the levels of detection and are fewer than 20 parts per billion. Therefore, selected solvents have minimal potential reaction effects on the botanical material, and are neither acidic nor alkaline. At the end of each production cycle, the processing plant is sealed so that solvents are constantly recycled and totally recovered. Electricity is the unique utility required to perform these systems and, even then, they consume little energy. There is no scope for the escape of the solvents, and even if some solvents come to escape, they pose no threat to the ozone layer because they do not contain chlorine. The waste product (biomass) from these plants is dry and “ecofriendly” to handle.
As the benefits of this procedure, we have the following:
the phytonic process is soft and its products are never damaged by exposure to temperatures over ambient because relatively low temperatures are employed;
vacuum stripping is necessary which, in other processes, leads to the loss of precious volatiles;
the process is performed completely at neutral pH, and in without oxygen, the products never suffer acid hydrolysis damage or oxidation;
the procedure is extremely selective, and offer a choice of operating conditions end products;
it requires a minimum amount of electrical energy;
it is less threatening to the environment;
no harmful emission in the atmosphere and the subsequent waste products (spent biomass) are inoffensive and pose no effluent disposal problems;
the solvents employed are neither toxic, nor flammable, or ozone-depleting;
the solvents are entirely recycled within the system.
In biotechnology, the utilization of the phytonics process is frequently employed to extract (e.g., for the production of antibiotics), herbal drug, food, EOs and flavor industries, and pharmacologically active products. It is particularly used to produce top-quality pharmaceutical-grade extracts, pharmacologically active intermediates, antibiotic extracts, and phytopharmaceuticals. However, the fact that it is used in all these areas prevents its use in other areas. The technique is being used to extract high-quality essential oils, oleoresins, natural food colors, flavors and aromatic oils from all types of plant material. The technique is also used in refining crude products obtained from other extraction processes. It provides extraction without wax or other contaminants. It helps in the removal of many biocides from contaminated biomass [50].
8.3 Liquid–Liquid extraction (partitioning)
Upon extraction of the solids and release of desired organics into the extraction solvent, the most common next step is a liquid–liquid extraction, taking advantage of mixing two (or sometimes three or even more that can establish two phases) non miscible solvents, for example, water and ether. The standard rule of thumb is that polar compounds go into polar solvents (e.g., amino acids, sugars, and proteins remain in water). To the contrary, the nonpolar components usually remain in the organic phase (e.g., steroids, terpenoids, waxes, and carotenoids are typically extracted into a solvent such as ethyl acetate).
It is important to minimize interference from compounds that may coextract with the target compounds during the extraction of plant material by conventional or by advanced methods. It is also needed to avoid contamination of the extract and to prevent decomposition of important metabolites or artifact formation as a result of extraction conditions or solvent impurities [3]. Regardless of the extracting procedure employed, the resulting solution should be filtered to withdraw whatever particulate matter. Due to the accompanying increased risk of formation of artifact and decomposition or isomerization of extract components plant extract should not be stored in the solvent for a long time at room temperature or in sunlight because [3].
9. Extraction of specific metabolites
The chemical investigation profile of a plant extract, fractionation of a crude extract is suitable to isolate the major classes of compounds from each other before further chromatographic analysis. One procedure based on varying polarity that might be used on an alkaloids-containing plant is indicated in Figure 10. The type and quantity of components to be separate into different fractions will, vary from plant to plant. Such procedure can be modified when labile substances are investigated [47].
Figure 10.
A general procedure for extracting fresh plant tissues and fractionating into different classes according to polarity.
9.1 Extraction of essential oils (EOs)
Essential oils (EOs) are concentrated aromatic hydrophobic oily volatile liquids characterized by a strong odor and produced by all plant organs [91]. They are obtained from raw material by several extraction techniques such as water or steam distillation, hydrodiffusion, solvent extraction, Soxhlet extraction, expression under pressure or cold pressing method, also known as scarification method, microwave-assisted extraction, microwave hydrodiffusion and gravity, supercritical fluid or subcritical water extractions. The best extraction method to use depends on the ease of evaporating (volatility) and the hydrophilicity or hydrophobicity (polarity) of the desired components [92, 93, 94, 95, 96]. However, the three most commonly applied techniques to extract EOs are Soxhlet, hydrodistillation, and SFE [97]. The extraction method chosen significantly affects the chemical composition of EOs [91]. Benmoussa and collaborators have recently found that the microwave hydrodiffusion and gravity (MHG) appeared like a rapid process, a green technology, and a desirable alternative protocol to enhance both the quality and the quantity of the EOs extracted from medicinal and aromatic plants [92].
9.2 Extraction of fats and oils
Lipids contain a broad category of non-polar molecules that are barely soluble or completely insoluble in water, but soluble in an organic solvent such as n-hexane, diethyl ether, chloroform, and alcohol [98]. Fats are triglycerides that are solid or semi-solid at room temperature, while oil is also triglycerides that are liquid or clear liquid at room temperature, however, their chemistry is determined by the degree of solubility. Fats and oil may be of vegetable, animal, and marine origin [99]. Oilseeds and fats production requires several units-operations, starting with a pre-treatment stage. It is often necessary to dry the sample before oil extraction using solvents because many organic solvents are not miscible with water and cannot easily penetrate the matrix and extraction would be inefficient [100]. The processing methods used are usually neither specific to lipids, nor insure 100% recovery of the lipid material because of the nature of the matrix. Diethyl ether and petroleum ether stands as favorite solvents in the case of crude fat because they are relatively non polar, hence extract most non-polar components [98].
Extraction process of edible oils may have negative effects on taste, stability, appearance or nutritional value, preserve tocopherols, and prevent chemical changes in the triacylglycerol. Fats and oil can be extracted from plants using conventional and advanced techniques that include hot water extraction, cold pressing, solvent extraction, high-pressure solvent extraction, microwave –assisted extraction, and supercritical fluid extraction [99]. Extraction of oil involves several mechanisms for removing a liquid from a solid such as leaching, washing, diffusion and dialysis [98]. In the case of palm oil (seeds of Elaeis oleifera), crude oil is obtained after a digestion step followed by a pressing stage. Digestion helps the rupture or breaking down the oil-bearing cells, thus releasing the palm oil in the fruit [101, 102, 103]. Enzyme-assisted extraction (EAE) is an efficient method to improve lipid extraction from several different biomasses such as soybean, sunflower, and microalgae [104, 105].
The main side reactions reported during oil processing are (i) trans fatty acid formation, (ii) cis-trans isomerization, (iii) and physical loss [99]. Before oilseeds processing, moisture must not exceed a certain limit to prevent growth of fungi and the occurring lipase formation, resulting in a free fatty acid increase [98].
9.3 Volatile organic compounds
Volatile organic compounds (VOCs) are odorant compounds emitted from plant tissues. Plants can produce a high diversity of VOCs. They are responsible for the distinct aroma of certain dried plants, including the tea, Camellia sinensis. VOCs can therefore be used as an indicator of tea quality [106, 107]. Several VOCs are emitted as a natural defense mechanism against arthropods and pathogen attacks [108, 109].
Hydro-distillation (HD), steam distillation (SD), simultaneous distillation solvent extraction (SDE), microwave-assisted hydro-distillation (MWHD), supercritical fluid extraction (SFE), purge and trap, and solid phase microextraction (SPME), are used to extract VOCs [110].
Verde and collaborators conducted a work to optimize the MAE of the volatile oil terpenes from Pterodon emarginatus fruits and characterize the volatile compounds. According to their study, MAE proved to be feasible with a particular interest in avoiding the need of organic solvents in volatile oil extraction from plants. They proved that a minimum amount of water could be enough to bring result in extraction. That green methodology appears to be an excellent alternative to extract terpenes from aromatic plants [111].
9.4 Alkaloids
The alkaloids are low molecular weight nitrogen-containing compounds found mainly in plants and a lesser extent in microorganisms and animals. They contain one or more nitrogen atoms, typically as primary, secondary, or tertiary amines, which usually confers basicity on the alkaloids. If the free electron pair on the nitrogen atom is not involve in mesomerism, the salt formation can occur mineral acids. This fundamental property of alkaloids is used in their extraction and further clan-up. According to the nature of the nitrogen-containing structure, alkaloids are classified as pyrrolidine, piperidine, quinoline, isoquinoline, indole, etc. [27].
Two methods may be used for alkaloids extraction. One is to basify the plant material using diethylamine or ammonia and extract with an organic solvent [112, 113]. Alkaloids are substances with a basic character and their solubility is a function of pH. They are soluble in low polar organic solvents in basic medium, while in acidic medium, they are soluble in water.
Alkaloids containing basic amines can be selectively extracted using a modified version of the classical “acid–base shake-out” method (Figure 11).
Figure 11.
General procedure to obtain alkaloidal extracts from crude plant material [114].
As recommendations, mineral acids and strong bases should be avoided in extracting alkaloids (and plant material in general) because of the risk of artifact formation [3, 114, 115].
9.4.1 Extraction of caffeine
Caffeine is a natural product found in Coffee, cocoa beans, kola nuts, and tea leaves in a substantial amount. Its efficient extraction from Coffee relies heavily on the properties of caffeine and other components present in Coffee. One of the most popular species of the genus whose seeds contains caffeine is Coffea arabica (Figure 12). Several methods can be used to extract caffeine, including Ultrasonic extraction, Heat Reflux extraction, and Soxhlet extraction. Heat Reflux extraction is commonly used methods to extract caffeine from Coffee [116]. The initial solvent used in the extraction of caffeine is water. Caffeine is sparingly soluble in water at ambient temperature (2 g/100 mL) but increasing when mixed in boiled water (100°C) with a yield of 66 g/ 100 mL. Meanwhile, the solubility of caffeine in chloroform, toluene, acetone and ethyl acetate is relatively high at ambient temperature [116, 117]. Caffeine is a weakly basic, white colorless powder in its anhydrous state.
Figure 12.
Chemical structure and a few data of caffeine.
There are several ways to remove caffeine from coffee. Here are few reported procedures:
9.4.1.1 Extraction procedure I: solvent extraction using dichloromethane (DCM)
Coffee seeds are firstly grounded and refluxed in an aqueous sodium carbonate solution for about 20 minutes under constant stirring. After filtration of the resulted mixture to filtrate is allowed for cooling at room temperature. The DCM is use to perform the partition of the aqueous filtrate. The process is repeated several times to extract more caffeine. The DCM fractions are then mixed with anhydrous sodium sulfate to remove water traces, the DCM-caffeine solution is filtered through reverse-phase filter paper, which will trap any water and residual matter. The DCM solution is allowed to evaporate and the white amorphous powder of caffeine is obtained [118].
The addition of sodium carbonate converts the protonated form of caffeine, which is naturally present in coffee, to its free caffeine form. During the extraction of caffeine, tannins being soluble in water and organic solvents can interfere with extraction. A weak base such as calcium carbonate or sodium sulphate can be added to break down tannins esters bonds into glucose and calcium or sodium salts of gallic acid, both of which will not be extracted into the organic solvent.
Some benefits are reported when using this method: caffeine is easily extracted from the final product after avoiding the use of flammable and toxic solvents. In this process, caffeine diffuses into supercritical CO2 with water. Coffee beans are introduced at the top while fresh CO2 is introducing at the bottom of an extractor vessel in a continuous extraction to remove caffeine. The recovery is accomplished in a separate absorption chamber containing water. Higher temperature and pressure are mandatory to obtain great yields. A pretreatment step is needed in this process. The addition of polar cosolvents affects cosolvent solute specific chemical or physical interactions. The extraction rate is accelerated by the solvent–cosolvent interaction and makes the extraction easier. The material is humidified with ultrapure water for prewetting, this will destroy the hydrogen bonds that link the caffeine to its natural matrix. Cell membrane swelling enhances solute diffusion. Subsequently, the quality of caffeine extracted can reach a purity >94%, which is generally the standard criteria for use in the soft drink and drug companies [119].
There are some benefits to use charcoal: it is cheaper, “green,” and ease to regenerate by heat and steam. The choice of active charcoal with the appropriate number of micropores and a specific area up to 1000 m2/gram is mandatory for good absorption performance.
Cleaned green coffee beans are firstly soak in water, and the caffeine and other soluble content transferred to the aqueous phase. During the filtration through the activated charcoal, solely caffeine will continue to migrate in water. The recovered and dried coffee beans are now decaffeinated [30].
9.4.2 Extraction of morphine
The poppy straw (Papaver somniferum capsules) produces a white sticky latex known as opium. Usually, two weeks after the petals fall from the bud farmers harvest and collect opium. To allow the viscous latex to ooze out slowly farmers generally use sharp blade to do two to five incisions into the pod’s skin. 24-hours after incisions of the pod, opium is then collected. This gummy latex, or opium (poppy tears), is a complex mixture containing at least 50 different alkaloids (Figure 11). Morphine is the major alkaloid, making up to 8–17% of the dry weight of opium. The chemical structure of morphine was established in 1925 despite de fact it has been used for centuries. Even if the immense majority of morphine continues to be harvested from the opium poppy, there are at least three classical processes (all old) for the extraction of morphine from simple starting material [120].
9.4.2.1 Extraction by Merck process
Cold water is used to treat the opium and the obtained aqueous solution concentrated until syrupy consistence. Powered sodium carbonate is added to precipitate hot and heated as long as ammonia given off; it is recommended that the solution remain alkaline to phenolphthalein and left aside four 24 hours at room temperature. After standing, the precipitate is filtered and cold water is use to wash several times until the wash-water become colorless. The precipitate is dissolved in alcohol at 85°C and the alcoholic solution is allowed for evaporation until dryness, and the residue is exhausted after neutralization with little amount of acetic acid. Decolorizing charcoal is used to treat the acidic solution and afterward precipitated with ammonia, avoiding excess is important. After filtration, the precipitate is washed and purified by crystallization in alcohol; concentration of the alcoholic mother-liquor yields a further quantity of morphine. This procedure was reported to be impossible to be consider for industrial scale because of the slight solubility of morphine is alcohol [120].
9.4.2.2 The Thiboumery and Mohr process
The gummy opium in divide into thin slices and treated with hot water thrice of its weight until obtain a homogeneous paste. After filtration the residue is pressed and treated again with thrice its weight in water. The resulted solutions are combined and allowed to evaporation until half their volume and poured into boiling milk of lime. One part of lime in ten parts of water should be used for four parts of opium; it is then filtered off again. The lime solutions are united and concentrated to a quantity twice the weight of the opium used. The solution is filtered, heated to boiling, and morphine is precipitated by adding ammonium chloride. The solution is filtrated after cooling at room temperature, and the precipitate is washed, then purified by solution in hydrochloric acid and crystallization of the morphine hydrochloride. It is an attractive process since there are no technical difficulties and the morphine is well separated from the secondary alkaloids. The morphine solutions are relatively clean; however, the yield might be bad. The contributory factors may be the oxidation of morphine in alkaline solution, and the fact that the lime always retains morphine [120].
9.4.2.3 The Roberson-Gregory process
Five to ten times its weight of cold distilled water is used to completely exhaust the opium. The resultant solution is evaporated to the consistency of a soft extract. The process is repeated with cold distilled water. This aqueous re-extraction causes impurities to precipitate, they are filtered off and the solution obtained is evaporated until its density is 10° Baumé. For each kilogram of opium, one hundred and twenty grams of calcium chloride are added to the boiling liquor, which is further diluted with an amount of cold water equal to its volume. A mixture of a precipitate of meconate and sulfate of calcium is thus formed and is filtered off. After filtration, the filtrate is once more concentrated to produce a new deposit which consist almost entirely of calcium meconate. After removal of the residue by filtration, the filtrate is left to stand for few days until it becomes a crystalline mass called “Gregory’s salt”. It is a mixture of hydrochloride and codeine hydrochloride. The crystals obtained are drained and then placed in a cloth and squeezed out in the presser. Successive crystallization is employed and each time animal charcoal is used to decolorize the solutions. To separate morphine to codeine, sufficiently pure crystals are dissolve in water and ammonia is therefore added to precipitate morphine while codeine remains in aqueous solution.
The first disadvantage of this procedure is that 20 to 25% of the morphine is left with the secondary alkaloids in the brown and viscous mother-liquids after filtration of the Gregory’s salt. The second drawback is that the hydrochloride of morphine and codeine crystallize in furry needles retains the mother-liquids in which the crystallization occurred. Several successive crystallization and subsequent recoveries are required for purification, which is a time-consuming process [120].
Later in 1957, an efficient method of extraction of morphine from poppy straw was developed by Mehltretter and Weakley. Water-saturated isobutanol containing 0.23% ammonia was used to extract morphine. Almost all the alkaloid was absorbed by passing off the raw opium through a cation exchange ions resin bed. Quantitative elution of morphine from the bed was achieved with dilute aqueous alkali. After neutralization and concentration, the crude morphine is obtained, and the eluate can be converted to hydrochloride pharmaceutical grade without difficulty. The general recovery of morphine was 90% [121].
Cooper and Nicola have reported recently a straightforward process for extraction of morphine with a good overall yield (Figures 11 and 13). Morphine and related alkaloids can be purified from opium resin and crude extracts by extraction in the following manner: first, soaking the resin with diluted sulfuric acid, which releases the alkaloids into solution. Either ammonium hydroxide or sodium carbonate then precipitates the alkaloids. The last step separates morphine from other opium alkaloids. Today, morphine is isolated from opium in relatively large quantities: over 1000 tons per year (Figure 14) [30].
Figure 13.
Extraction of raw opium from poppy straw.
Figure 14.
Extraction protocol of morphine from raw opium by Cooper and Nicola [30].
Till date, morphine is used as a powerful painkiller to alleviate severe pain by acting straightaway on the brain. It also possesses euphoric and hallucinatory effects. Morphine can also be chemically converted by an acetylation reaction using acetic anhydride and pyridine to create a much more potent form of the narcotic drug known as heroin [30].
9.5 Glycosides
Glycosides are relatively polar, and their polarity depends on both the number and type of sugar moieties attached to the aglycone. Cardiac glycosides have bulky steroidal aglycone, which are soluble in chloroform. However, most glycosides are extracted using polar solvents like acetone, methanol, ethanol, water or mixtures of these solvents. When extraction in done using water as solvent, enzymatic breakdown can happen. This will be avoid by using boiling water or add important proportions of alcohol or ammonium sulfate to the extract. In some cases, it may be the hydrolytic separation of the aglycone and sugar before or after extraction [122, 123].
9.6 Total phenolic and total flavonoids content
Phenolic compounds are well-known phytochemicals found in almost all pla