Open access peer-reviewed chapter

Antibiotic Use in Poultry Production and Its Effects on Bacterial Resistance

Written By

Christian Agyare, Vivian Etsiapa Boamah, Crystal Ngofi Zumbi and Frank Boateng Osei

Submitted: 20 March 2018 Reviewed: 07 June 2018 Published: 05 November 2018

DOI: 10.5772/intechopen.79371

From the Edited Volume

Antimicrobial Resistance - A Global Threat

Edited by Yashwant Kumar

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Abstract

A surge in the development and spread of antibiotic resistance has become a major cause for concern. Over the past few decades, no major new types of antibiotics have been produced and almost all known antibiotics are increasingly losing their activity against pathogenic microorganisms. The levels of multi-drug resistant bacteria have also increased. It is known that worldwide, more than 60% of all antibiotics that are produced find their use in animal production for both therapeutic and non-therapeutic purposes. The use of antimicrobial agents in animal husbandry has been linked to the development and spread of resistant bacteria. Poultry products are among the highest consumed products worldwide but a lot of essential antibiotics are employed during poultry production in several countries; threatening the safety of such products (through antimicrobial residues) and the increased possibility of development and spread of microbial resistance in poultry settings. This chapter documents some of the studies on antibiotic usage in poultry farming; with specific focus on some selected bacterial species, their economic importance to poultry farming and reports of resistances of isolated species from poultry settings (farms and poultry products) to essential antibiotics.

Keywords

  • bacteria
  • antibiotic resistance
  • antibiotics
  • antimicrobials
  • poultry

1. Introduction

Antibiotic resistance (AR) which is defined as the ability of an organism to resist the killing effects of an antibiotic to which it was normally susceptible [1] and it has become an issue of global interest [2]. This microbial resistance is not a new phenomenon since all microorganisms have an inherent capacity to resist some antibiotics [3]. However, the rapid surge in the development and spread of AR is the main cause for concern [4]. In recent years, enough evidence highlighting a link between excessive use of antimicrobial agents and antimicrobial resistance from animals as a contributing factor to the overall burden of AR has emerged [5]. The extent of usage is expected to increase markedly over coming years due to intensification of farming practices in most of the developing countries [6]. The main reasons for the use of antibiotics in food-producing animals include prevention of infections, treatment of infections, promotion of growth and improvement in production in the farm animals [7, 8].

Poultry is one of the most widespread food industries worldwide. Chicken is the most commonly farmed species, with over 90 billion tons of chicken meat produced per year [9]. A large diversity of antimicrobials, are used to raise poultry in most countries [10, 11, 12]. A large number of such antimicrobials are considered to be essential in human medicine [13, 14]. The indiscriminate use of such essential antimicrobials in animal production is likely to accelerate the development of AR in pathogens, as well as in commensal organisms. This would result in treatment failures, economic losses and could act as source of gene pool for transmission to humans. In addition, there are also human health concerns about the presence of antimicrobial residues in meat [15, 16], eggs [17] and other animal products [18, 19].

Generally, when an antibiotic is used in any setting, it eliminates the susceptible bacterial strains leaving behind those with traits that can resist the drug. These resistant bacteria then multiply and become the dominating population and as such, are able to transfer (both horizontally and vertically) the genes responsible for their resistance to other bacteria [1, 20]. Resistant bacteria can be transferred from poultry products to humans via consuming or handling meat contaminated with pathogens [21]. Once these pathogens are in the human system, they could colonize the intestines and the resistant genes could be shared or transferred to the endogenous intestinal flora, jeopardizing future treatments of infections caused by such organisms [5, 22, 23, 24].

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2. Use of antibiotic in animal production

Antimicrobials’ use in animal production dates as far back as the 1910 when due to shortage of meat products, workers carried out protests and riots across America [25]. Scientists at that time started looking for means of producing more meat at relatively cheaper costs; resulting in the use of antibiotics and other antimicrobial agents [26]. With the global threat of antibiotic resistance and increasing treatment failures, the non-therapeutic use of antibiotics in animal production has been banned in some countries [8, 27, 28, 29]. Sweden is known to be the first country to ban the use of antimicrobials for non-therapeutic purposes between 1986 (for growth promotion) and 1988 (for prophylaxis) [27]. This move was followed by Denmark, The Netherlands, United Kingdom and other European Union countries [27]. These countries also moved a step further and banned the use of all essential antibiotics as prophylactic agents in 2011 [30].

Several other countries have withdrawn the use of some classes of antibiotics or set up structures that regulate the use of selected antibiotics in animal production [29]. Despite these developments, it is currently estimated that over 60% of all antibiotics produced are used in livestock production, including poultry [6, 31].

The use of antibiotics in poultry and livestock production is favorable to farmers and the economy as well because it has generally improved poultry performance effectively and economically but at the same time, the likely dissemination of antibiotic resistant strains of pathogenic and non-pathogenic organisms into the environment and their further transmission to humans via the food chain could also lead to serious consequences on public health [32].

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3. Antimicrobial resistance

Bacteria counteract the actions of antibiotics by four well-known mechanisms, namely; enzyme modification, alteration in target binding sites, efflux activity and decreased permeability of bacterial membrane [33]. This expression of resistance towards antibiotics by bacteria could either be intrinsic or acquired. Intrinsic resistance is due to inherent properties within the bacteria chromosome such as mutations in genes and chromosomally inducible enzyme production [34], whereas acquired resistance could be due to the transmission of resistance genes from the environment and/or horizontally transfer from other bacteria [35, 36].

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4. Antibiotic resistance of some selected organisms in poultry

4.1. Staphylococcus species

The bacterial genus Staphylococcus is a Gram-positive cocci and a facultative anaerobe which appears in clusters when viewed under the microscope [37]. They are etiological agents of staphylococcosis, pododermatitis (bumblefoot) and septicaemia which affect mostly chicken and turkeys. Coagulase-negative species have also been implicated in human and animal infections [38, 39].

β-lactams were considered the first line of drugs for treatment of staphylococcal infections but due to emergence of high level of resistance to these and other drugs, there are currently very few drugs available for treatment of these infections [40]. Methicillin resistant Staphylococcus aureus (MRSA), now known as a superbug, is resistant to almost every available antibiotic used against Staphylococcus [41].

A study to detect the presence of MRSA in broilers, turkeys and the surrounding air in Germany reported the prevalence of MRSA in air as high as 77% in broilers compared to 54% in Turkeys. Ten different spa types were identified with spa type t011 and clonal complex (CC) 398 being the most prevalent. It was also found that for every farm, the same sequence types were present in both the birds and the environment [42]. This pattern of resistance was also reported in India with 1.6% of staphylococcal isolates containing mecA resistant gene [43].

In Africa, studies carried out in Ghana and Nigeria have shown that livestock-associated Staphylococci are susceptible to amoxicillin/clavulanic acid, amikacin, ciprofloxacin, gentamycin and cephalexin [39, 44], whereas in the US, most of the staphylococcal isolates were susceptible to rifampin, cotrimoxazole, gentamycin, vancomycin and chloramphenicol [45, 46]. It is worth noting that most of these organisms showed a high level of resistance to oxacillin and tetracycline, which would be disastrous if these oxacillin-resistant strains are transferred to humans [39, 44, 45].

4.2. Pseudomonas species

Pseudomonas is a genus of Gram-negative, aerobic bacteria that belongs to the family Pseudomonadaceae [47]. The genus Pseudomonas is ubiquitous in soil, water and on plants. It consists of 191 subspecies belonging to species groups including P. fluorescens, P. pertucinogena, P. aeruginosa, P. chlororaphis, P. putida, P. stutzeri and P. syringae. Pseudomoniasis, which is an opportunistic P. aeruginosa infection, is common in poultry birds like chickens, turkeys, ducks, geese and ostriches where infections in eggs destroy embryos [48].

P. aeruginosa causes respiratory infection, sinusitis, keratitis/keratoconjuctivitis and septicemia and responsible for pyogenic infections, septicemia, endocarditis and lameness along with many diverse diseases [49]. Infections may occur through skin wounds, contaminated vaccines and antibiotic solutions or needles used for injection. The disease may be systemic, affecting multiple organs and tissues or localized in tissues as infraorbital sinus or air sacs producing swelling of the head, wattles, sinuses and joints in poultry birds. P. aeruginosa has been isolated from many poultry farms and birds worldwide [49].

A study carried out in Ghana show that P. aeruginosa isolated from poultry litter were all susceptible to levofloxacin in the range of 20–100% and nearly 75% demonstrated intermediate susceptibility to aztreonam. The organisms showed resistance to cephalosporins, carbapenems, penicillins, quinolones, monobactam and aminoglycoside. Metallo β-Lactamase encoding genes (blaIMP, blaVIM) were not detected in any of the isolates but the class 1 integron which is known to carry multiple antibiotic resistant genes were detected in 89.4% of the multi-drug resistant strains [50]. This is contrary to a report by Zhang and his Colleagues [51], who identified the blaVIM gene in P. aeruginosa and P. putida from chicken that resembled corresponding regions in clinical isolates of P. aeruginosa. These isolates were resistant to all β-lactam antibiotics tested, including meropenem, imipenem, aztreonam, and ceftazidime [33, 51].

Another study in Nigeria reported that the P. aeruginosa isolates were highly resistant to β-lactams, tetracycline, tobramycin, nitrofurantoin and sulfamethoxazole-trimethoprim, while ofloxacin, imipenem and ertapenem were highly effective against the bacterial pathogens [52].

In Pakistan, a study which investigated the causative agents for necropsy in chicken, recorded a 28% prevalence for P. aeruginosa. These isolates were found to be 100% resistant towards ceftriaxone, meropenem, ciprofloxacin, erythromycin and colistin, while 60% sensitivity was observed against ampicillin sulbactam, ceftazidime, cefoperazone and rifampicin. Isolates exhibited variable multidrug resistance patterns to other antibiotics [53].

4.3. Escherichia species

Escherichia coli is a Gram-negative bacterium that has been known for ages to easily and frequently exchange genetic information through horizontal gene transfer with other related bacteria. Hence, it may exhibit characteristics based on the source of isolation. E. coli is a commensal organism living in the intestines of both humans and animals. However, some strains have been reported to cause gastrointestinal illnesses [54]. Tetracycline which is commonly used in poultry has been reported to be one of the drugs bacteria are most resistant to. There is a reported tetracycline resistance in poultry even without the administration of this antibiotic [21].

A study carried out on fecal isolates of E. coli in the Netherlands showed that there is a high level of multidrug resistance occurring in broilers, turkeys while majority of those from laying hens were susceptible. It was observed that the isolates from birds had high rates of resistance to amoxycillin alone and others had resistance to amoxicillin as well as oxytetracycline, streptomycin, sulfamethoxazole and trimethoprim. [55].

E. coli had a prevalence of 46.98% among the other bacteria isolated in Ghana. All isolates showed some degree of resistance to ceftriaxone (1.34%), cefotaxime (0.67%), gentamycin (2.01%), cotrimoxazole (1.34%), tetracycline (2.01%) and ampicillin (3.36%) [56]. Resistant genes have been found in E. coli isolates from Nigeria and these include bla-TEM (85%), sul2 (67%), sul3 (17%), aadA (65%), strA (70%), strB (61%), catA1 (25%), cmlA1 (13%), tetA (21%) and tetB (17%) which conveyed resistance to the following antibiotics; tetracycline (81%), sulfamethoxazole (67%), streptomycin (56%), trimethoprim (47%), ciprofloxacin (42%), ampicillin (36%), spectinomycin (28%), nalidixic acid (25%), chloramphenicol (22%), neomycin (14%) gentamicin (8%). In this study the isolates were susceptible to amoxicillin-clavulanate, ceftiofur, cefotaxime, colistin, florfenicol and apramycin. Class 1 and 2 integrons were found in five (14%) and six (17%) isolates, respectively, while one isolate contained both classes of integrons. There is that suggestion that poultry production environments represent important reservoirs of antibiotic resistance genes such as qnrS that may spread from livestock production farms to human populations via manure and water [57].

4.4. Salmonella species

Salmonella spp. are Gram-negative, facultative anaerobic, non-spore forming, usually motile rods belonging to the Enterobacteriaceae family, which are found in the alimentary tract of animals [37, 58]. Fecal shedding allows Salmonella to be transmitted among birds in a flock. Salmonella spp. is widespread in poultry production. Prevalence varies considerably depending on country and type of production as well as the detection methods applied. It is known to be the etiological agent responsible for salmonellosis by Salmonella spp. in both humans and animals. Food-borne salmonellosis caused still occurs throughout the world [58]. The risk factors associated with Salmonella infections and contamination in broiler chickens include contaminated chicks, size of the farm and contaminated feed and these risk increase when feed trucks are parked near the entrance of the workers’ change room and when chicken are fed with meals [59, 60]. It also depends on age of the chicken, animal health, survival of organism in the gastric barrier, diet and genetic constitution of the chicken could also affect the colonization ability of Salmonella spp. in poultry [61].

Pullorum disease in poultry is caused by the S. pullorum. Transmission of the disease in birds can be vertical (transovarian) but also occurs through direct or indirect contact with infected birds via respiratory route or fecal matter or contaminated feed, water, or litter. Antimicrobials used to treat pullorum disease are furazolidone, gentamycin sulfate and antimetabolites (sulfadimethoxine, sulfamethazine and sulfamerazine) [62].

Salmonella spp. have increasingly been isolated from poultry with prevalence of 2.7% in Brazil and the most common isolates were Salmonella enteritidis (48.8%), S. infantis (7.6%), S. typhimurium (7.2%), and S. heidelberg (6.4%). All the isolated strains were resistant to at least one class of antimicrobial and 53.2% showed multidrug resistance to three or more classes, with streptomycin (89.2%), sulfonamides (72.4%), florfenicol (59.2%), and ampicillin (44.8%) [63].

Salmonella spp. are one of the commonest microbial contaminants in the poultry industry. In Ghana, there is high prevalence rate of 44.0% in poultry with main isolates being S. kentucky (18.1%), S. nima (12.8%), S. muenster (10.6%), S. enteritidis (10.6%) and S. virchow (9.6%). Resistance of these isolates to the various antibiotics were nalidixic acid (89.5%), tetracycline (80.7%), ciprofloxacin (64.9%), sulfamethazole (42.1%), trimethoprim (29.8%) and ampicillin (26.3%).

4.5. Streptococcus species

Streptococcus is Gram-positive bacteria. Streptococcus gallolyticus is a common member of the gut microbiota in animals and humans; however, being a zoonotic agent, it has been reported to cause mastitis in cattle, septicemia in pigeons, and meningitis, septicemia, and endocarditis in humans [64]. A study carried out in Japan isolated Streptococcus gallolyticus from pigeons with septicaemia. Most of the isolates were susceptible to vancomycin, penicillin G and ampicillin, while some were resistant to tetracycline, doxycycline and lincomycin. All the isolates were resistant to tetracycline had tet(M) and/or tet(L) and/or tet(O) genes [65].

4.6. Campylobacter species

Campylobacter jejuni and Campylobacter coli are the most prevalent disease causing species of the genus Campylobacter. They are mostly responsible for foodborne gastroenteritis in humans [66, 67, 68]. Campylobacteriosis is often associated with handling of raw poultry or eating of undercooked poultry meat [69]. Cross-contamination of raw poultry to other ready-to-eat foods via the cook’s hands or kitchen utensils has been reported. Erythromycin is usually the drug of choice for the treatment of Campylobacter infections [68]. However, fluoroquinolones, gentamicin, and tetracycline are also clinically effective in treating Campylobacter infections when antimicrobial therapy is required [70].

Resistance of C. jejuni and C. coli isolates to fluoroquinolones, tetracycline, and erythromycin has been reported. The increased resistance is partly due to the wide use of these antimicrobials in animal husbandary, especially in poultry [71, 72].

A study carried out by Elz’bieta and his colleagues, in their quest to compare the prevalence and genetic background of antimicrobial resistance in Polish strains of C. jejuni and C. coli isolated from chicken carcasses and children reported a slight difference in resistance between human and chicken strains. The isolated Campylobacter strains were found to be resistant to gentamycin, tetracycline, ampicillin, ciprofloxacin and erythromycin and tet(O) gene and mutations in the gyrA genes were found to be associated with the observed antibiotic resistance in the study [73].

Another study carried out in Kenya isolated thermophilic Campylobacter species (C. jejuni and C. coli) from feces and clocal swabs of chicken. These isolates showed a high rate of resistance to nalidixic acid, tetracycline and ciprofloxacin of 77.4, 71.0 and 71.0%, respectively. Low resistance (25.8%) was detected for gentamicin and chloramphenicol and 61.3% of C. jejuni isolates exhibited multidrug resistance and 54.5% of the C. jejuni isolates possessed the tet(O) gene whereas all of C. coli had the tet(A) gene [74].

C. jejuni and C. coli are the predominant species of Campylobacter usually isolated from poultry farms. In Ghana, other species such as Campylobacter lari, Campylobacter hyo-intestinalis and C. jejuni sub sp. doylei have been isolated from poultry. These organisms have been found to be resistant to β-lactams, quinolones, aminoglycosides, erythromycin, tetracycline, chloramphenicol and trimethoprim-sulfamethoxazole and all isolated species were sensitive to imipenem [75, 76].

4.7. Yersinia species

It is a Gram-negative non-spore-forming rod, a psychrotrophic bacterium and able to survive and multiply at cold temperatures. Poultry meat is one of the most important sources of Yersinia spp. infections in humans. Yersinia enterocolitica is the predominant specie mostly isolated from poultry and poultry products [77]. In humans, Y. enterocolitica is an enteric pathogen which commonly causes acute enteritis associated with fever, bloody diarrhea and inflammation of lymph nodes. Contaminated food is one of the main sources of yersiniosis in humans [77].

Y. enterocolitica is widely distributed in nature and animals; food and environment are routinely contaminated with this organism. Major reservoir of Y. enterocolitica is swine. However, Y. enterocolitica has been frequently isolated from poultry and ready-to-eat foods [78]. A study in Iran reported a prevalence rate of Y. enterocolitica of 30% of among chicken meat samples [79]. Yersinia isolates (16%) from chicken and beef meat samples were mostly resistant to cephalotin (98%) and ampicillin (52%) [80].

Y. enterocolitica isolated from poultry raw meat and retailed meats in Poland were classified as biotype 1A and exhibited moderate ability of producing biofilms and ystB was the predominant virulence gene. In biofilms, a multi-system that include poor antibiotic penetration, nutrient limitation and slow growth, adaptive stress responses, and formation of persister cells are hypothesized to constitute the organisms’ resistance to antibiotics [81].

4.8. Clostridium species

Clostridium is a genus of Gram-positive obligate anaerobic bacteria which includes several significant human pathogens. Spore of Clostridium normally inhabits soil and intestinal tract of animals and humans [82]. Common infections caused by Clostridia include botulism caused by C. botulinum¸ pseudomembranous colitis caused by C. difficile, cellulitis and gas gangrene caused by C. perfringens, tetanus caused by C. tetani and fatal post-abortion infections caused by C. sordellii [83].

High-dose penicillin-G remains sensitive to Clostridia species and thus widely used to treat Clostridial infections. Clostridia species such as welchii and tetani respond to sulfonamides [82]. Tetracyclines, carbapenems, metronidazole, vancomycin and chloramphenicol are effective options for treatment of Clostridia infections [84].

C. perfringens is known to cause necrotic enteritis in poultry. Bacitracin or virginiamycin is an effective treatment option when administered in the feed or drinking water. C. colinum is responsible for ulcerative enteritis. Bacitracin and penicillins are the most effective drugs in the treatment and prevention of this infection [85, 86].

A study in Egypt, identified 125 isolates of C. perfringens from clinical cases of necrotic enteritis in broiler chickens from 35 chicken coops and the all isolates were resistant to gentamycin, streptomycin, oxolinic acid, lincomycin, erythromycin and spiramycin. Over 95% of isolates were resistant to sulfamethoxazole-trimethoprim, doxycycline, perfloxacin, colistin and neomycin. Most of the isolates were susceptible to amoxicillin, ampicillin, fosfomycin, florfenicol and cephradine [85].

Thirty strains of C. perfringens isolated from chickens with necrotic enteritis in Korea were found to susceptible to ampicillin, amoxicillin/clavulanic acid, cephalothin, cefepime, chloramphenicol, cefoxitin, ceftiofur, florfenicol and penicillin but resistant to gentamycin, neomycin, streptomycin, apramycin and colistin [87]. This trend of resistance was similar to that observed in 43 C. perfringens isolates from the ileum of 5-week old broiler chicken in Taiwan. Most of the C. perfringens isolates were susceptible to amoxicillin, bacitracin and enrofloxacin but resistant to erythromycin, lincomycin and chlortetracycline [88].

4.9. Bacillus species

Bacillus is a genus of Gram-positive, obligate aerobic or facultative anaerobic rod shaped bacteria of the phylum firmicutes. Bacillus spp. include both free-living non-parasitic and parasitic pathogenic species [89]. Medically significant species include B. anthracis which causes anthrax and B. cereus which causes food poisoning [90]. Other infections caused by Bacilli spp. include pneumonia, endocarditis, ocular and musculoskeletal infections. Antibiotics usually used for Bacillus infections include vancomycin, imipenem, ciprofloxacin, gentamycin, tetracycline, chloramphenicol, clindamycin and erythromycin. Most Bacillus spp. have been found to be resistant to broad spectrum cephalosporins and ticarcillin-clavulanate [91].

In a study involving 18 strains of B. cereus isolated from raw and processed poultry meat from supermarkets in Iasi county, all the isolates were found to be resistant to penicillin, amoxicillin, amoxicillin-clavulanate, colistin, cefoperazone, sulfamethizole and metronidazole but sensitive to erythromycin, cotrimoxazole, tylosin, flumequine, kanamycin, gentamycin, enrofloxacin, oxolinic acid, apramycin, tetracycline and doxacilin. All B. cereus isolates were resistant to nearly half of tested antibiotics [92]. This pattern of resistance was also observed in 44 strains of B. cereus isolated from chicken and chicken products in the Jammu region of India. All isolates were resistant to penicillin G but sensitive to streptomycin. Over 60% of isolates were resistant to amoxicillin, ampicillin and carbenicillin [93].

4.10. Mycobacterium species

Mycobacteria are acid-fast, aerobic, nonmotile of bacteria of the genus Mycobacterium [94]. Mycobacteria are widespread organisms that live in water and food sources and can colonize their hosts without showing any adverse signs and symptoms. Pathogenic mycobacterial species including M. tuberculosis, M. bovis, M. africanum, M. macroti cause tuberculosis whiles M. leprae is responsible for leprosy. Mycobacteria spp. are naturally resistant to penicillin and mostly susceptible to clarithromycin and rifamycin [95].

A study in Bangladesh identified three Mycobacterium isolates from 80 poultry droppings and all isolates were found to be resistant to rifampicin but highly susceptible to azithromycin, ciprofloxacin, streptomycin and doxycycline. One isolate was identified as multi-drug resistant [96].

4.11. Klebsiella species

Klebsiella is a genus of non-motile, Gram-negative, oxidase-negative, rod-shaped bacteria with a prominent polysaccharide capsule and belong to the family Enterobacteriaceae [97]. Klebsiella species are found everywhere in nature including soil, plants, insect, humans and other animals [98]. Infections caused by Klebsiella spp. include septicaemia, meningitis, urinary tract infections, pneumonia, diarrhea [97]. Common pathogenic Klebsiella in humans and animals include K. pneumoniae, K. oxytoca and K. variicola [99]. Antibiotics commonly used in the treatment of Klebsiella infections include third-generation cephalosporins, carbapenems, aminoglycosides and quinolones [100].

A study in Langa, South Africa identified 102 sub-species of K. pneumonia (96 K. ozaenae and 6 K. rhinoscleromatis strains) from 17 free-range chicken samples. The isolates exhibited high level of resistance towards ampicillin (66.7%), nalidixic acid (61.8%), tetracycline (59.8%) and trimethoprim (50.0%) but highly susceptible towards gentamycin (3.9%) and ciprofloxacin (4.8%). Almost 40% of the isolates were found to be multi-drug resistant K. pneumonia strains [99]. Similar trend of resistance was observed among 77 K. pneumoniae isolates from poultry birds in Ekiti-state, Nigeria. The isolates showed high level of resistance towards tetracycline (100%), amoxicillin (94.8%), cotrimoxazole (94.8%) and augmentin (85.7%) [98].

4.12. Enterococcus species

Enterococcus is a large genus of Gram-positive diplococci, lactic acid-producing bacteria of the phylum Firmicutes [101]. Commonly found species include Enterococcus faecalis and Enterococcus faecium [102]. Notable infections caused by Enterococci include urinary tract infections, bacteremia, meningitis, endocarditis [103]. Antibiotics active against Enterococci include ampicillin, penicillin, nitrofurantoin and vancomycin [104]. Enterococci often possess intrinsic resistance towards β-lactam antibiotics and aminoglycosides. However, resistance of Enterococci to vancomycin has been reported in several studies [105, 106, 107].

A study in Czech Republic identified 228 enterococcal isolates from the intestinal tract of poultry. These isolates were found to be highly resistant to tetracycline (80%), erythromycin (59%) and ofloxacin (51%) but exhibited low resistance to ampicillin (3%) and ampicillin/sulbactam (3%) [105]. A similar trend of resistance was reported among 163 Enterococcal isolates from poultry litter in the Abbotsford area of British Columbia, Canada. The identified enterococcal isolates were found to be highly resistant to lincomycin (80.3%), tetracycline (65.3%), penicillin (61.1%) but showed low resistance towards to nitrofurantoin (3.8%), daptomycin (3.5%) and gentamycin (0.8%) [108]. There is a high possibility of multi-drug resistant enterococci in animal meat and fecal matter being transferred to humans [106].

4.13. Proteus species

Proteus is a genus of Gram-negative Proteobacteria which is widely distributed as saprophytes [109]. They are mainly found in decomposing animal matter, sewage, manure, mammalian intestine, human and animal fecal matter. They are mainly opportunistic pathogens responsible for nosocomial urinary and septic infections [110]. Three species, namely, P. vulgaris, P. mirabilis and P. penneri are the only opportunistic species responsible for human infections. Most strains of P. mirabilis are sensitive to ampicillin and cephalosporins whereas P. vulgaris strains are not sensitive to these antibiotics [109].

A study in Iran identified 54 P. mirabilis isolates from chicken intestines and 54 P. mirabilis isolates were screened for antimicrobial susceptibility to 13 antimicrobial agents. None of the P. mirabilis isolates in this study were found to be resistant to gentamycin. Over 90% of isolates were resistant to nalidixic acid, doxycycline and tetracycline. Less than a quarter of isolates were resistant to norfloxacin, ampicillin, amikacin and ceftriaxone. Nearly 96% of the isolates were resistant to at least two or more antibiotics. One isolate exhibited resistance to 10 antibiotics whereas three and five isolates were resistant to nine and seven antibiotics, respectively. The results showed that chicken could be a source of antibiotic resistant and multi-drug resistant P. mirabilis strains and these resistant strains can cause worldwide problem both for veterinary sector and public health [111].

A similar trend of antibiotic resistance was observed in 36 P. mirabilis isolates from chicken droppings from commercial poultry farms in Bangladesh. Nearly 95% of the isolates were resistant to tetracycline followed by nalidixic acid (89%) and almost 20% of the isolates were found to be resistant to ciprofloxacin and 84% of the isolates exhibited multidrug resistance [112].

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5. Other species of importance

Infections from other bacterial species could also result in the use of antibiotics. These include Mycoplasmosis (caused by Mycoplasma gallisepticum, Mycoplasma meleagridis and Mycoplasma synoviae) [86], Pasteurella multocida and Haemophilus gallinarum infections [62, 113]. These infections usually require the use broad spectrum antibiotics including tylosin, aureomycin, terramycin, gallimycin, penicillin, erythromycin, sulfadimethoxine, sulfathiazole and other sulfa drugs administered either in the feed, drinking water or by injections [62].

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6. Conclusion

Several bacterial species are the major causes of infections in poultry and other animal husbandry. Most of these infections are linked to foodborne outbreaks, live animal contact, poor hygiene, and environmental exposure. With the emergence of antimicrobial resistance, the pathogenicity and virulence of these organisms have increased and treatment options are diminishing and also more expensive. Multidrug resistant bacteria have been found in poultry, poultry products, carcasses, litter and fecal matter of birds and these pose a risk to both handlers, consumers and a threat to global and public health. The above information also calls for increased surveillance measures and monitoring of antibiotic usage in both animal husbandry and humans throughout the world.

References

  1. 1. Madigan MT, Martinko JM, Bender KS, Buckley FH, Stahl DA. Brock Biology of Microorganisms. 14th ed. Illinois: Pearson International; 2014. p. 1006
  2. 2. Antimicrobial Resistance Global Report on Surveillance. Geneva: World Health Organi-zation; 2014: 256. Retrieved from: http://www.who.int/drugresistance/documents/surveillancereport/en/ on 15th April, 2018
  3. 3. Hugo WB, Russel AD. Pharmaceutical Microbiology. 6th ed. Oxford: Blackwell Science Ltd; 1998. p. 514
  4. 4. Aarestrup FM, Wegener HC, Collignon P. Resistance in bacteria of the food chain: Epidemiology and control strategies. Expert Review of Anti-Infective Therapy. 2008;6:733-750
  5. 5. Marshall BM, Levy SB. Food animals and antimicrobials: Impacts on human health. Clinical Microbiology Reviews. 2011;24:718-733
  6. 6. Van Boeckel TP, Brower C, Gilbert M, Grenfell BT, Levin SA, Robinson TP, Teillant A, Laxminarayan R. Global trends in antimicrobial use in food animals. Proceedings of the National Academy of Sciences. 2015;112:5649-5654
  7. 7. Mathew AG, Liamthong S, Lin J. Evidence of Int 1 transfer between Escherichia coli and Salmonella typhi. Food Biology. 2009;6(8):959-964
  8. 8. Castanon JIR. History of the use of antibiotic as growth promoters in European poultry feeds. Poultry Science. 2007;86:2466-2471
  9. 9. Food and Agricultural Organization. FAO Publications Catalogue 2017. United Nations: Food and Agricultural Organization; 2017. Retrieved from http://www.fao.org/3/b-i6407e.pdf on 14th April, 2018
  10. 10. Landers TF, Cohen B, Wittum TE, Larson EL. A review of antibiotic use in food animals: Perspective, policy, and potential. Public Health Reports. 2012;127(1):4-22
  11. 11. Sahoo KC, Tamhankar AJ, Johansson E, Lundborg CS. Antibiotic use, resistance development and environmental factors: A qualitative study among healthcare professionals in Orissa, India. BioMedical Central Public Health. 2010;10:629
  12. 12. Boamah VE, Agyare C, Odoi H, Dalsgaard A. Antibiotic practices and factors influencing the use of antibiotics in selected poultry farms in Ghana. Journal of Antimicrobial Agents. 2016;2:120. doi: 10.4172/2472-1212.1000120
  13. 13. World Health Organization Model List of Essential Medicines. Geneva: World Health Organization; 2010:1-43. Retrieved from http://www.who.int/medicines/publications/essentialmedicines/en/ on 13th April, 2018
  14. 14. World Health Statistics 2017: Monitoring Health for the Sustainable Development Goals. Geneva: World Health Organization; 2017. Retrieved from http://apps.searo.who.int/PDS_DOCS/B5348.pdf on the 10th April, 2018
  15. 15. Mirlohi M, Aalipour F, Jalali M. Prevalence of antibiotic residues in commercial milk and its variation by season and thermal processing methods. International Journal of Environmental Health Engineering. 2013;2:41
  16. 16. Darwish WS, Eldaly EA, El-Abbasy MT, Ikenaka Y, Nakayama S, Ishizuka M. Antibiotic residues in food: The African scenario. Japanese Journal of Veterinary Research. 2013;61:S13-S22
  17. 17. Goetting V, Lee KA, Tell LA. Pharmacokinetics of veterinary drugs in laying hens and residues in eggs: A review of the literature. Journal of Veterinary Pharmacology and Therapy. 2011;34:521-556
  18. 18. Addo KK, Mensah GI, Aning KG, Nartey N, Nipah GK, Bonsu C, Akyeh ML, Smits HL. Microbiological quality and antibiotic residues in informally marketed raw cow milk within the coastal savannah zone of Ghana. Tropical Medicine and International Health. 2011;16:227-232
  19. 19. Mehdizadeh S, Kazerani HR, Jamshidi A. Screening of chloramphenicol residues in broiler chickens slaughtered in an industrial poultry abattoir in Mashhad, Iran. Iranian Journal of Veterinary Science and Technology. 2010;2:25-32
  20. 20. Laxminarayan R, Duse A, Wattal C, Zaidi AKM, Wertheim HFL, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C, So AD, Bigdeli M, Tomson G, Woodhouse W, Ombaka E, Peralta AQ, Qamar FN, Mir F, Kariuki S, Bhutta ZA, Coates A, Bergstrom R, Wright GD, Brown ED, Cars O. Antibiotic resistance–The need for global solutions. Lancet Infectious Diseases. 2013;13:1057-1098
  21. 21. van, den Bogaard AE, Stobberingh EE. Epidemiology of resistance to antibiotics: Links between animals and humans. International Journal of Antimicroial. Agents. 2000;14:327-335
  22. 22. Hall MAL, Dierikx CM, Stuart JC, Voets GM, van den Munckhof MP. Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strains. Clini-cal Microbiology and Infection. 2011;17(6):873-880
  23. 23. Jakobsen L, Kurbasic A, Skjøt-rasmussen L, Ejrnæs K, Porsbo LJ, Pedersen K, Jensen LB, Emborg H, Agersø Y, Olsen KEP, Aarestrup FM, Frimodt-møller N, Hammerum AM. Escherichia coli isolates from broiler chicken meat, broiler chickens, pork and pigs share phylogroups and antimicrobial resistance with community-dwelling. Foodborne Pathogens and Disease. 2010;7:537-547
  24. 24. de Leener E, Martel A, de Graef EM, Top J, Butaye P, Haesebrouck F, Willems R, Decostere A. Molecular analysis of human, porcine, and poultry Enterococcus faecium isolates and their erm (B) genes. Applied Environmental Microbiology. 2005;71:2766-2770
  25. 25. Ogle M. In Meat We Trust: An Unexpected History of Carnivore America. New York: Houghton Mifflin Harcourt Publishing Company; 2013. p. 384
  26. 26. Dibner JJ, Richards JD. Antibiotic growth promoters in agriculture: History and mode of action. Poultry Science. 2005;84:634-643
  27. 27. Cogliani C, Goossens H, Greko C. Restricting antimicrobial use in food animals: Lessons from Europe. Microbe. 2011;6:274-279
  28. 28. European Union. Ban on antibiotics as growth promoters in animal feed enters into effect. Regulation. Brussels: European Union. 2006. 1P:05:1687
  29. 29. Choct M. Alternatives to in-feed antibiotics in monogastric animal industry. ASA Technical Bulletin. 2001;30:1-7
  30. 30. Maron DF, Smith TJS, Nachman KE. Restrictions on antimicrobial use in food animal production: An international regulatory and economic survey. Globalization and Health. 2013;9:48
  31. 31. van Boeckel TP, Gandra S, Ashok A, Caudron Q, Grenfell BT, Levin SA, Laxminarayan R. Global antibiotic consumption 2000 to 2010: An analysis of national pharmaceutical sales data. Lancet Infectious Diseases. 2014;14:742-750
  32. 32. Apata DF. Antibiotic resistance in poultry. International Journal of Poultry Science. 2009;8:404-408
  33. 33. Bassetti M, Merelli M, Temperoni C, Astilean A. New antibiotics for bad bugs: Where are. we? Annual Clinical Microbiology and Antimicrobials. 2013
  34. 34. Davies J. Microbes have the last word. European Molecular Biology Organization Reports. 2007;8:616-621
  35. 35. McDermott PF, Walker RD, White DG. Antimicrobials: Modes of action and mechanisms of resistance. International Journal of Toxicology. 2003;22:135-143
  36. 36. Randall LP, Cooles SW, Osborn MK, Piddock LJV, Woodward MJ. Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK. Journal of Antimicrobial Chemotherapy. 2004;53:208-216
  37. 37. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria. 3th ed. Cambridge, UK: Cambridge University Press; 2009. p. 331
  38. 38. Koksal F, Yasar H, Samasti M. Antibiotic resistance patterns of coagulase-negative Staphylococcus strains isolated from blood cultures of septicemic patients in Turkey. Microbiology Research. 2009;164:404-410
  39. 39. Boamah VE, Agyare C, Odoi H, Adu F, Gbedema S, Dalsgaard A. Prevalence and antibiotic resistance of coagulase-negative Staphylococci isolated from poultry farms in three regions of Ghana. Infection and Drug Resistance. 2017;10:175-183
  40. 40. Mamza SA, Egwu GO, Mshelia GD. Beta-lactamase Escherichia coli and Staphylococcus aureus isolated from chickens in Nigeria. Veterinary Italian Journal. 2010;46:155-165
  41. 41. Stapleton PD, Taylor PW. Methicillin resistance in Staphylococcus aureus. Science Progress. 2007;85:57-72
  42. 42. Friese A, Schulz J, Zimmermann K, Tenhagen BA, Fetsch A, Hartung J, Rösler U. Occur-rence of livestock-associated methicillin-resistant Staphylococcus aureus in Turkey and broiler barns and contamination of air and soil surfaces in their vicinity. Applied Environmental Microbiology. 2013;79:2759-2766
  43. 43. Bhedi KR, Nayak JB, Brahmbhatt MN, Roy A. Detection and molecular characterization of methicillin-resistant Staphylococcus aureus obtained from poultry and poultry house environment of Anand district, Gujarat, India. International Journal Current Microbiology and Applied Sciences. 2018;7:867-872
  44. 44. Suleiman A, Zaria LT, Grema HA, Ahmadu P. Antimicrobial resistant coagulase positive Staphylococcus aureus from chickens in Maiduguri, Nigeria. Sokoto Journal of Veterinary Science. 2013;11:51-55
  45. 45. Waters AE, Contente-Cuomo T, Buchhagen J, Liu CM, Watson L, Pearce K, Foster JT, Bowers J, Driebe EM, Engelthaler DM, Keim PS, Price LB. Multidrug-resistant Staphylococcus aureus in US meat and poultry. Clinical Infectious Diseases. 2011;52:1227-1230
  46. 46. Abdalrahman LS, Stanley A, Wells H, Fakhr MK. Isolation, virulence, and antimicrobial resistance of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin sensitive Staphylococcus aureus (MSSA) strains from Oklahoma retail poultry meats. International Journal of Environmental Research and Public Health. 2015;12:6148-6161
  47. 47. Skerman SV, McGowan V, Sneath P. Approved Lists of Bacterial Names (Amended). Approved List of Bacteria Names. Washington DC: ASM Press; 1989. p. 196
  48. 48. de Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB. Bergey’s Manual of Systematic Bacteriology. New York: Springer: 2009. p. 1450
  49. 49. Sams AR. Poultry Meat Processing. Boca Raton: CRC Press; 2001. p. 345
  50. 50. Odoi H. Isolation and Characterization of Multi-Drug Resistant Pseudomonas aeruginosa from Clinical, Environmental and Poultry Litter Sources in Ashanti Region of Ghana (MPhil Thesis). Kumasi: Kwame Nkrumah University of Science and Technology; 2016
  51. 51. Zhang R, Liu Z, Li J, Lei L, Yin W, Li M, Wu C, Walsh TR, Wang Y, Wang S, Wua Y. Presence of VIM-positive Pseudomonas species in chickens and their surrounding environment. Antimicrobial Agents and Chemotherapy. 2017;61:1-5
  52. 52. Aniokette U, Iroha CS, Ajah MI, Nwakaeze AE. Occurrence of multi-drug resistant Gram-negative bacteria from poultry and poultry products sold in Abakaliki. Journal of Agricultural Science and Food Technology. 2016;2:119-124
  53. 53. Sharma S, Galav V, Agrawal M, Faridi F, Kumar B. Multi-drug resistance pattern of bacterial flora obtained from necropsy samples of poultry. Journal of Animal Health and Production. 2017;5:165-171
  54. 54. Tenaillon O, Skurnik D, Picard B, Denamur E. The population genetics of commensal Escherichia coli. National Review of Microbiology. 2010;8:207-217
  55. 55. van den Bogaard AE, London N, Driessen C, Stobberingh EE. Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. Journal of Antimicrobial Chemotherapy. 2001;47(6):763-771
  56. 56. Yao GM. Prevalence and Antibiotic Resistance of Salmonella sp., Shigella sp. and Escherichia coli in Fresh Retail Chicken in the Accra Metropolis. Accra: University of Ghana; 2015
  57. 57. Adelowo OO, Fagade OE, Agersø Y. Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, Southwest Nigeria. Journal of Infections in Developing Countries. 2014;8:1103-1112
  58. 58. Bell C, Kyriakides A. Salmonella. A Practical Approach to the Organism and its Control in Foods. Oxford: Blackwell Science; 2007. p. 338
  59. 59. Marin C, Balasch S, Vega S, Lainez M. Sources of Salmonella contamination during broiler production in eastern Spain. Preventive Veterinary Medicine. 2011;98:39-45
  60. 60. Arsenault J, Letellier A, Quessy S, Normand V, Boulianne M. Prevalence and risk factors for Salmonella spp. and Campylobacter spp. faecal colonization in broiler chicken and Turkey flocks slaughtered in Quebec, Canada. Preventive Veterinary Medicine. 2007;81:250-264
  61. 61. Cosby DE, Cox NA, Harrison MA, Wilson JL, Buhr RJ, Fedorka-Cray PJ. Salmonella and antimicrobial resistance in broilers: A review. Journal of Applied Poultry Research. 2015;24:408-426
  62. 62. Msoffe PL, Aning KG, Byarugaba DK, Mbuthia PG, Sourou S, Cardona C, Bunn DA, Nyaga PN, Njagi LW, Maina AN, Kiama SG. Handbook of Poultry Diseases Important in Africa. CRSP: A Project of the Global Livestock; 2009. p. 83
  63. 63. Medeiros MAN, de Oliveira DCN, Rodrigues DP, de Freitas DRC. Prevalence and antimicrobial resistance of Salmonella in chicken carcasses at retail in 15 Brazilian cities. Pan American Journal of Public Health. 2011;30:555-560
  64. 64. de Herdt P, Devriese L, de Groote B, Ducatelle R, Haesebrouck F. Antibiotic treatment of Streptococcus bovis infections in pigeons. Avian Pathology. 1993;22:605-615
  65. 65. Nomoto R, Tien LHT, Sekizaki T, Osawa R. Antimicrobial susceptibility of Streptococcus gallolyticus isolated from humans and animals. Japanese Journal of Infectious Diseases. 2013;66:334-336
  66. 66. Sackey BA, Mensah P, Collison E, Sakyi-Dawson E. Campylobacter, Salmonella, Shigella and Escherichia coli in live and dressed poultry from Accra metropolitan. International Journal of Food Microbiology. 2001;71:21-28
  67. 67. Wimalarathna HML, Richardson JF, Lawson AJ, Elson R, Meldrum R, Maiden MCJ, Mccarthy ND, Sheppard SK. Widespread Acquisition of Antimicrobial Resistance among Campylobacter Isolates from UK Retail Poultry and Evidence for Clonal Expansion of Resistant Lineages. BioMedical Central Microbiology; 2013
  68. 68. Acheson D, Allos BM. Campylobacter jejuni infections: Update on emerging issues and trends. Clinical Infectious Diseases. 2001;32(8):1201-1206
  69. 69. Altekruse SF, Stern NJ, Fields PI, Swerdlow DL. Campylobacter jejuni–An emerging foodborne pathogen. Emerging Infectious Diseases. 1999;5:28-35
  70. 70. Moore JE, Deborah C, Dooley JSG, Fanning S, Lucey B, Matsuda M, Mcdowell DA, Mégraud FB, Millar C, O’Mahony R, O’Riordan L, O’Rourke M, Rao JR, Rooney PJ, Sails A, Whyte P. Campylobacter. Veterinary Research. 2005;36:351-382
  71. 71. Wilson IG. Antibiotic resistance of Campylobacter in raw retail chickens and imported chicken portions. Epidemiology and Infection. 2003;131:1181-1186
  72. 72. Randall LP, Ridley AM, Cooles SW, Sharma M, Sayers AR, Pumbwe L, Newell DG, Piddock LJV, Woodward MJ. Prevalence of multiple antibiotic resistance in 443 Campylobacter spp. isolated from humans and animals. Journal of Antimicrobial Chemo-therapy. 2003;52:507-510
  73. 73. Rożynek E, Dzierżanowska-Fangrat K, Korsak D, Konieczny P, Wardak S, Szych J, Jarosz M, Dzierżanowska D. Comparison of antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from humans and chicken carcasses in Poland. Journal of Food Protection. 2008;71:602-607
  74. 74. Nguyen TNM, Hotzel H, Njeru J, Mwituria J, El-Adawy H, Tomaso H, Neubauer H, Hafez HM. Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya. Gut Pathology. 2016;8:1-9
  75. 75. Kumar VA, Steffy K, Chatterjee M, Sugumar M, Dinesh KR, Manoharan A, Karim S, Biswas R. Detection of oxacillin-susceptible mecA-positive Staphylococcus aureus isolates by use of chromogenic medium MRSA ID. Journal of Clinical Microbiology. 2013;51(1):318-319
  76. 76. Karikari AB, Obiri-Danso K, Frimpong EH, Krogfelt K.A. Antibiotic resistance of Campylobacter recovered from faeces and carcasses of healthy livestock. Biomed Research International. 2017; 4091856
  77. 77. Annamalai T, Venkitanarayanan K. Expression of major cold shock proteins and genes by Yersinia enterocolitica in synthetic medium and foods. Journal of Food Protection. 2005;68:2454-2458
  78. 78. Rahman A, Bonny TS, Stonsaovapak S, Ananchaipattana C. Yersinia enterocolitica: Epidemiological studies and outbreaks. Journal of Pathogens. 2011:1-11
  79. 79. Sirghani K, Zeinali T, Jamshidi A. Detection of Yersinia enterocolitica in retail chicken meat, Mashhad, Iran. Journal of Pathogens. 2018;2018:1286216
  80. 80. Dallal MMS, Doyle MP, Rezadehbashi M, Dabiri H, Sanaei M, Modarresi S, Bakhtiari R, Sharifiy K, Taremi M, Zali MR, Sharifi-Yazdi MK. Prevalence and antimicrobial resistance profiles of Salmonella serotypes, Campylobacter and Yersinia spp. isolated from retail chicken and beef, Tehran, Iran. Food Control. 2010;21(4):388-392
  81. 81. Zadernowska A, Chaje W. Prevalence, bio film formation and virulence markers of Salmonella sp. and Yersinia enterocolitica in food of animal origin in Poland. LWT-Food Science and Technology. 2017;75:552-556
  82. 82. Péchiné S, Collignon A. Immune responses induced by Clostridium difficile. Anaerobe. 2016;41:68-78
  83. 83. Num SM, Useh NM. Clostridium : Pathogenic oles, industrial uses and medicinal prospects of natural products as ameliorative agents against pathogenic species. Jordan Journal of Biological Sciences. 2014;7(2):81-94
  84. 84. Banawas SS. Clostridium difficile infections: A global overview of drug sensitivity and resistance mechanisms. Biomed Research International. 2018:1-9
  85. 85. Osman KM, Elhariri M. Antibiotic resistance of Clostridium perfringens isolates from broiler chickens in Egypt. Review of Science and Technology. 2013;32(2):841-850
  86. 86. Nhung NT, Chansiripornchai N, Carrique-Mas JJ. Antimicrobial resistance in bacterial poultry pathogens: A review. Frontiers in Veterinary Science. 2017;4:1-17
  87. 87. Park JY, Kim S, Oh JY, Kim HR, Jang I, Lee HS, Kwon YK. Poultry science. 2015;94:1158-1164
  88. 88. Fan YC, Wang CL, Wang C, Chen TC, Chou CH, Tsai HJ. Incidence and antimicrobial susceptibility to Clostridium perfringens in premarket broilers in Taiwan. Avian Disease. 2016;60(2):444-449
  89. 89. Slepecky RA, Hemphill HE. The genus Bacillus-nonmedical. In: Balows A, Truper HG, Dworkin M, Harder W, Schleifer KH, editors. The Prokaryotes. 2nd ed. New York: Springer; 2009. p. 562
  90. 90. Fagerlund A, Lindbäck T, Granum PE. Bacillus cereus cytotoxins Hbl, Nhe and CytK are secreted via the sec translocation pathway. BioMed Central Microbiology. 2012;10:304
  91. 91. Reboli AC, Bryan CS, Farrar WE. Bacteremia and infection of a hip prosthesis caused by Bacillus alvei. Jounal of Clinical Microbiology. 1989;27(6):1395-1396
  92. 92. Floriştean V, Cretu C, Carp-Cărare M. Bacteriological characteristics of Bacillus Cereus isolates from poultry. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. 2007;64:1-2
  93. 93. Bashir M, Malik MA, Javaid M, Badroo GA. Prevalence and characterization of Bacillus cereus in meat and meat products in and around Jammu region of Jammu and Kashmir, India. International Journal of Current Microbiology and Applied Sciences. 2017;6(12):1094-1106
  94. 94. Rastogi N, Legrand E, Sola C. The mycobacteria: An introduction to nomenclature and pathogenesis. Review of Science Technology. 2001;20(1):21-54
  95. 95. Barrow WW. Treatment of mycobacterial infections pathogenesis intracellular parasitism. Scientific and Technical Review of the Office International des Epizooties (Paris). 2001;20(1):55-70
  96. 96. Reza M, Lijon M, Khatun M, Islam M. Prevalence and antibiogram profile of Myco-bacterium spp. in poultry and its environments. Journal of Advanced Veterinary and Animal Research. 2015;2(4):458
  97. 97. Podschun R, Ullmann U. Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors. Journal of Clinical Microbiology. 1998;11(4):589-603
  98. 98. Ajayi AO, Egbebi AO. Antibiotic susceptibility of Salmonella typhi and Klebsiella pneumoniae from poultry and local birds in Ado-Ekiti, Ekiti-state. Nigeria. Annals of Biological Research. 2011;2(3):431-437
  99. 99. Fielding BC, Mnabisa A, Gouws PA, Morris T. Antimicrobial-resistant Klebsiella species isolated from free-range chicken samples in an informal settlement. Archives of Medical Science. 2012;8(1):39-42
  100. 100. van Duin D, Bonomo RA. Ceftazidime/avibactam and ceftolozane/tazobactam: Second-generation β-lactam/β-lactamase inhibitor combinations. Clinical Infectious Diseases. 2016;63(2):234-241
  101. 101. Teixeira LM, De Janeiro R, Merquior VLC. Enterococcus. In: Filippis I, McKee M, editors. Molecular Typing in Bacterial Infections. New York: Springer Science; 2013. p. 17-27
  102. 102. Gilmore MS, Clewell DB, Courvalin P. The Enterococci: Pathogenesis, Molecular Biology and Antibiotic Resistance. Washington, USA: ASM Press; 2002. p. 484
  103. 103. Fisher K, Phillips C. The ecology, epidemiology and virulence of Enterococcus. Micro-biology. 2009;155(6):1749-1757
  104. 104. Zhanel GG, Laing NM, Nichol KA, Palatnick LP, Noreddin A, Hisanaga T, Johnson JL, Hoban DJ. Antibiotic activity against urinary tract infection (UTI) isolates of vancomycin-resistant Enterococci (VRE): Results from the 2002 north American vancomycin resistant Enterococci susceptibility study (NAVRESS). Journal of Antimicrobial Chemotherapy. 2003;52(3):382-388
  105. 105. Kolář M, Pantůček R, Bardoň J, Vágnerová I, Typovská H, Válka I, Doškař J. Occurrence of antibiotic-resistant bacterial strains isolated in poultry. Veterinary Medicine (Praha). 2002;47(2-3):52-59
  106. 106. Vignaroli C, Zandri G, Aquilanti L, Pasquaroli S, Biavasco F. Multidrug-resistant enterococci in animal meat and faeces and co-transfer of resistance from an Enterococcus durans to a human Enterococcus faecium. Current Microbiology. 2011;62(5):1438-1447
  107. 107. Oguttu WJ. Antimicrobial Drug Resistance of Enteric Bacteria from Broilers Fed Antimicrobial Growth Enhancers and Exposed Poultry Abattior Workers [Thesis]. Pretoria: University of Pretoria; 2007
  108. 108. Furtula V, Jackson CR, Farrell EG, Barrett JB, Hiott LM, Chambers PA. Antimicrobial resistance in Enterococcus spp. isolated from environmental samples in an area of intensive poultry production. International Journal of Environmental Research and Public Health. 2013;10(3):1020-1036
  109. 109. Różalski A, Torzewska A, Moryl M, Kwil I, Maszewska A, Ostrowska K, Drzewiecka D, Zabłotni A, Palusiak A, Siwińska M, Staçzek P. Proteus spp. – An opportunistic bacterial pathogen–Classification, swarming growth, clinical significance and virulence factors. Folia Biologica et Oecologica. 2012;8(1):1-17
  110. 110. Ahmed DA. Prevalence of Proteus spp. in some hospitals in Baghdad City. Iraqi Journal of Science. 2015;56(1):665-672
  111. 111. Nemati M. Antimicrobial resistance of Proteus isolates from poultry. European Journal of Experimental Biology. 2013;3(6):499-500
  112. 112. Nahar A, Siddiquee M, Nahar S, Anwar KS, Ali SI, Islam S. Multidrug resistant-Proteus mirabilis isolated from chicken droppings in commercial poultry farms : Bio-security concern and emerging public health threat in Bangladesh. Biosafety and Health Education. 2014;2(2):120-125
  113. 113. McEwen SA, Fedorka-Cray PJ. Antimicrobial use and resistance in animals. Clinical Infectious Diseases. 2002;34(3):S93-S106

Written By

Christian Agyare, Vivian Etsiapa Boamah, Crystal Ngofi Zumbi and Frank Boateng Osei

Submitted: 20 March 2018 Reviewed: 07 June 2018 Published: 05 November 2018