Distinctive uncertainties of the objects and processes in the oil and gas industry.
\r\n\tHealth and mental health issues of both children and adults and evidence-based treatments will be included. The types of sexual violence that occur and prevention efforts that have – or have not – been made to address the occurrence of these types of violence will be covered.
\r\n\r\n\tCultural and governmental policies, as well as legal and jurisdiction issues to address victims of these crimes, will also be incorporated in the book. For instance, Meagan’s Law and its worthiness in protecting children will be incorporated as well as the Federal program to reimburse victims of online child pornography and the legal entanglements and ramifications of that program.
\r\n\r\n\tThe typology of offenders and the effectiveness of treatment will also be addressed.
\r\n\tFinally, the direction of prevention strategies, treatment needs for both victims and offenders, and policy issues to move the field forward, particularly in terms of research, will be presented. The field of sexual violence has made significant strides in the past 45 years in terms of understanding sexually deviant behavior, the impact on children who then experience the symptoms of that trauma in adulthood, how to effectively interview sexually victimized children, and finally, treatment and social mores that make disclosure possible and recovery hopeful.
A history of development of the oil and gas industry all over the world, and in the Russian Federation, is impressive. Since 1930s large oil and gas fields have been opened; a huge number of oil refining and petrochemical factories are constructed. In recent years, the role of the gas branch has essentially increased; pipeline transport, thanks to which the basic part of Russia’s territory is provided with gas, oil and mineral oil, has actively developed; export of these products is carried out; there has been development of sea deposits. Hydrocarbon reservoirs, pipeline transport, oil refining and petrochemical factories, various storehouses of oil and gas, sea platforms and terminals and so on are examples of objects of modeling in oil and gas systems (OGS)—see Figure 1.
\nObjects of modeling.
Technological processes of oil and gas branches are various. As a matter of fact, it is all a spectrum of processes from hydrocarbon extraction to end-product production. There are geological and geophysical researches; drilling; developing of hydrocarbon reservoirs (both on land, shelves and on the sea); pipeline transport and oil and gas storage; refining and chemistry. The end production of oil and gas manufacturing is used in majority branches of the modern economy. Unfortunately, up-to-date claims for deposits of hydrocarbons are the reasons for international conflicts.
\nFeatures which are necessary for consideration for the creation of control systems by technological processes and at construction are peculiar to the oil and gas branch. So technological processes are continuous, and objects are difficult and demand at the management level of performing synergistic researches. Objects of oil and gas manufacturing are technologically dangerous; therefore, the role of systems’ safety and ecological monitoring is significant. The initial information possesses are characterized by the high level of uncertainty generated by natural factors.
\nThe automated dispatching control (ADC) meets the requirements of continuous technological processes control (ADC is the heterogeneous man–machine control system of the technological process integrating the dispatcher with an information-operating system, providing automatic information gathering, transfer, processing and display [1, 2]).
\nTheoretical bases for creating heterogeneous control systems are at the formation stage. Effective ACD operation in general depends on the quality of modeling objects and managerial processes. Problems of modeling for oil and gas systems should be considered for two levels:
problems of technological process control taking into account features of oil and gas manufacture and.
problems of monitoring and prediction of the integrated metrics, providing safe and competitive development of the OGS enterprises.
In such a manner, the systemic uncertainty inherent in oil and gas technologies due to the specificity of the objects under study leads to the need for modeling oil and gas systems, the goal of which is ultimately to manage risks at all levels of the hierarchy and all stages of the life cycle [1, 2, 3, 4, 5, 6, 7, 8, 9, 10].
\nThe problems posed are quite sophisticated, due to the complexity of the systems being studied, the operation of which is clearly non-linear. And at the same time, it is highly an actual one, taking into account the noted role of the OGS in the economy of modern world.
\nThe proposed probabilistic approaches, applicable in the system’s life cycle, help to answer the main question: “What rational measures should lead to expected effects without wasted expenses, when, by which controllable and uncontrollable conditions and costs?”
\nModeling demands the analysis of specificity for OGS, estimating existing uncertainties. Prominent features of objects of oil and gas manufacture, characterizing uncertainties and complexity of modeling are presented in Table 1.
\nObject or process | \nDistinctive factors (uncertainty) and applicable modeling tool | \n
---|---|
Geological structures (it is the isolated area of the earth shell, differing from the cross-border regions for the tectonic behavior, i.e. specific combination of the geological formations, its bedding and structuring conditions) | \nThe text and cartographical information symbiosis is used. The most popular tasks: correlation analysis, cluster analysis, association theory, interpolation theory. The spatial data modeling with the variograms evaluation (the spatial correlation measure) and other estimations. Natural phenomena modeling – one of the most progressive lines of the modern science. It is based on the digital models of the geological data combined with the spatial databases. The computer modeling tasks in the practical geology are solved with help of the modeling software packages. Actual tendencies of the geostatistics are connected with the development of: spatial analogues of the Monte-Carlo methods; approaches based on the multipoint statistics; hybrid models with artificial intelligence algorithms application; with additional information of the varied type and applications in the images processing and transfer area and others [11]. | \n
The Oil (it is typified as the oil disperse system) | \nIn the oil disperse system the behavior principles and physical–chemical properties in the molecular or disperse state can be quite differ from each other, and this is the reason of the non-linear response while changing of the external input character and scale. Then the phase transitions occur, and system properties are changed in a qualitative manner. This field researches show that oil systems are structured at the nanoscale level, what creates the basics for the new technologies development in the oil and gas industry. So the phase transitions are possible with the aggregate state changing. This is the object of the synergistic analysis; also the physical–chemical analysis models are applied; fractal analysis is used. | \n
Hydrocarbons reservoirs | \nThey were formed for millions years. Recently the count of hypothesizes about the hydrocarbon reservoirs origin increased, and generally speaking the self-organizing processes are typical for the oil and gas reservoirs. | \n
Hydrocarbons reservoir rock (it is mine rock containing the voids, i.e. the pores, cavities and others and having the potential to store and filtrate the fluids) | \nPorosity, permeability are the key indexes for the estimation task solution. The deformations are typical for the reservoir rocks. In certain cases it is needed to take into account the non-Newtonian fluid and to use the rheological models. The most dependencies have the non-linear character. The initial information has the statistical character, then the mathematical statistics and probabilistic modeling apparatus is actively applied. The percolation task is also has its special features. | \n
Processes of the petrochemical industry and oil refining | \nNon-linear processes with catalysts’ application, and its activity, are varied with time. Most wide-spread modeling and engineering evaluations systems are actively used for the project design as well as the calculations providing in management. | \n
Management of the oil production process (Intelligent field, i.e. I-field) | \nWhile solving the problem of the hydrocarbons production on the reservoir the task of the adjusted management with Kalman filter application. In classic case the adjusted management task supposes the object model correction with control input generation. Toward to the hydrocarbons reservoir the uncertainty is increased with the changing of the object characteristics while the reservoir development process. The tasks of the estimation and identification are widely used and applied. | \n
Management in the emergency situations and accidents | \nIn the oil disperse system occur the negative phenomena, which are connected with the phase transitions in technological processes of the oil refining, hydrocarbons reservoirs development, wells drilling and other processes of the oil and gas industry. These phenomena may include: the asphaltenes, paraffins and salts sedimentations, gas hydrates formation and others. Physical–chemical analysis of the oil disperse systems is supposed to be the key point in development of the decisions support systems in the emergency situations. Combined to the experimental researches practice the computer modeling at the molecular level is carried. Based on the results the type of the catastrophe is evaluated and the order parameter is identified. Finally the recommendations to the management in the emergency situations are developed. | \n
Distinctive uncertainties of the objects and processes in the oil and gas industry.
The performed analysis has revealed prominent features of uncertainties for separate objects of oil and gas manufacture and has shown that probabilistic modeling, models for estimations and identifications, a method of Monte-Carlo, is widely and successfully applied for solving problems of technological process control. The nature of uncertainty of processes and objects of oil and gas manufacture is various; that is in many respects caused by long processes of hydrocarbon formation. Therefore, the occurrence of technology of evolutionary modeling as often named synergistic analysis (with the theory of non-linear systems and the self-organizing processes, the determined chaos, fractal analysis, etc.) has considerably expanded possibilities of the researches of the natural uncertainty of oil and gas manufacture.
\nEvolutionary processes as a development basis, actively acted not only the system analysis in a control context, but also for the decision of problems at level of organizational-economic management. At this level, the nature of uncertainty is connected with many criteria. In Figure 2, the evolution of risk-oriented criteria is shown: from economic criteria to competitiveness.
\nEvolution of risk-oriented criteria.
In different areas, the heterogeneous threats for complex systems are inevitable. The uncertainties in the system’s life cycle are usual. Different problems, connected with evaluations, comparisons, selections, controls, system analysis and optimization, are solved by the probabilistic modeling of processes according to system engineering standards (general–ISO/IEC/IEEE 15288, ISO 9001, IEC 60300, 61,508, CMMI и т.д. and specific for the oil and gas industry ISO 10418, 13,702, 14,224, 15,544, ISO 15663, ISO 17776 etc.). The saved-up experience confirms the high importance of scientific system researches based on probabilistic modeling. For example, in general cases, prediction and optimization are founded on modeling different processes. Any process is a repeated sequence of consuming time and resources for outcomes’ receiving in all application areas. From the probability point of view, the moments for any activity beginning and ending are random events on the timeline. In practice, a majority of timed activities is repeated during the system’s life cycle (estimations, comparisons, analysis, rationale, etc.) [1, 2, 3, 4, 5, 6, 7, 8, 9, 10]. See some problems that are due to be and can be solved by the mathematical modeling of processes according to ISO/IEC/IEEE 15288 “Systems and software engineering. System life cycle processes” in Figure 3. The applications of models allows one to manage rationally risks, raise the quality and safety of oil and gas systems and at the expense of them be successful in the market—see in Figure 4 the example of formalized problems which are solved on the basis of probabilistic modeling in the life cycle [6].
\nThe problems that are due to be and can be solved by probabilistic modeling processes.
Examples of formalized problems which are solved on the base of probabilistic modeling.
The summary of the analysis of existing approaches is presented in the next section.
\nAn existing risk control concept tries to consider different uncertainties. But in the application for various areas, the results of information gathering and processing are not used purposefully for modeling, because as the used models of risk prediction that are used in a majority of complex systems, are specific, results and interpretations are not comparable. A universal objective scale of measurement is not established yet. Moreover, the terms “acceptable quality” and “admissible risk” should be defined on a probability scale level only in dependence with corresponding methods and precedents (considering system analogue). For heterogeneous threats, an analytical rationale of the balanced preventive measures of system integrity support at limitations on admissible risks and resources cannot be solved in many cases. The probabilistic modeling, aimed at pragmatic effects, helps to prove probability levels of” acceptable quality” and “admissible risk” for different systems in uniform interpretation, creates techniques to solve different problems for quality and helps in risk optimization. It supports making-decisions in quality and safety and/or helps to avoid wasted expenses in the system’s life cycle—see the proposed purposeful way in Figure 5, based on dozens of probabilistic models and software tools [6]. There are proposed universal metrics for system processes: probabilities of success or failure during a given period for an element, subsystem and system. A calculation of these metrics within the limits of the offered probability space built on the basis of the theory for random processes allows one to predict quality and risks on a uniform probability scale, quantitatively proving comprehensive levels of acceptable quality and admissible risks from “precedents cases.” The prediction of risks can use widely safety monitoring data and statistics. In practice, an application of the proposed model and method allows a customer to formulate better justified requirements and specifications, a developer to implement them rationally without wasted expenses and a user to use the system’s potential in the most effective way [1–12].
\nThe proposed way to support making-decisions in quality and safety.
In a general case, a probabilistic space (
The descriptions for some from the proposed probabilistic models and methods for their transformations, adaptations, applications and result interpretations are the following.
\nProblems of item content analysis are everywhere for any oil and gas systems in their life cycle. Pipes and pipelines, the equipment (e.g., fountain armature, columned heads and welded tanks), monolithic walls of buildings and the constructions, to be checked in the presence of emptiness, can be considered as such items—see Figure 6.
\nExamples of item content analysis.
For solving some problems of item content analysis, the existing probabilistic model “information faultlessness after checking” may be used by renaming input and output [6]. For example, for estimating non-destructive testing, the probability of soundness of the checked item (renamed) may be estimated instead of the probability of information faultlessness during the required term (according to referenced model [6]). A soundness of the checked item means the zero of defects (or anomalies) after non-destructive testing during the given term.
\nWhat about the effectiveness of non-destructive testing methods for some technical items?
\nExample 1: Let an application of some instruments of non-destructive testing be planned in the applications to check 10,000 conditional items (the items can be meters of pipes, square meters of walls in storehouses and so on). The operator using instruments forms a system for non-destructive testing. Speed of testing equals 5000 items a day. Taking into account the human factor, a frequency of first-type errors (when the absence of defect [anomaly] is accepted as defect [anomaly]) equals one error a week. The mean time between second-type errors for the system (when real defect [anomaly] does not come to light) is equal once a month. The non-destructive testing is performed permanently for 10 days. It needs to estimate the maximum density of defects (anomalies) for which the probability of soundness of the checked 10,000 conditional items is more than 0.90.
\nResults of probabilistic modeling have shown that the required density is about 0.02%, that is, 2 defects (anomalies) on 10,000 items. In addition it is expedient to notice that since density of defects about 1%, the probability of soundness is stabilized at level 0.88. It does not fall as less, because first-type and second-type errors seldom occur in example 1.
\nExample 2: Continuing example 1, it needs to prove minimum speed of non-destructive testing, the checked volume for which the probability of soundness of 10,000 conditional items will exceed 0.95 at continuous work within 8 h of working hours.
\nThe results of probabilistic modeling are reflected in Figure 7.
\nThe way for rationale speed of non-destructive testing.
The analysis shows that the found rational speed is about 1100 items per hour. And the part of defects after the control in the checked-up volume of 10,000 items will be 0.0008% against the primary 0.02%. It can be interpreted: at the checked volume of 1,00,000 items (i.e., in 10 times more primary 10,000, when quantity of defects is 20), the average residual quantity of defects will not exceed 1. It means that under the second example conditions, 19 from 20 defects will be revealed in time with probability 0.95 and more.
\nThe probabilistic approaches for modeling “black box” and complex structures operating in conditions of heterogeneous threats are proposed.
\nThere are two general technologies proposed of providing protection from critical influences on the system: technology 1 is the periodical diagnostics of system integrity (without the continuous monitoring between diagnostics) and technology 2 is the continuous monitoring between periodical diagnostics added to technology 1—see Figure 8.
\nSome accident events for technology 2 (left – “Correct operation”, right – “a loss of integrity” during Treq.).
Technology 1 is based on periodical diagnostics of system integrity, which is carried out to detect danger source penetration from threats (destabilizing factors) into a system or the consequences of negative influences. The lost system integrity can be detected only as a result of diagnostics, after which system recovery starts. Dangerous influence on a system is acted upon step by step: at first, a danger source penetrates into a system and then after its activation begins to influence. System integrity cannot be lost before a penetrated danger source is activated. Danger from threats (destabilizing factors) is considered to be realized only after a danger source has influenced a system.
\nTechnology 2, unlike the previous one, implies that operators alternating each other trace system integrity between diagnostics (the operator may be a man or a special device or their combination). In case of detecting a danger source an operator recovers system integrity. The ways of integrity recovering are analogous to the ways of technology 1. Faultless operator’s actions provide the neutralization of a danger source trying to penetrate into a system. When operators alternate a complex diagnostic is held. A penetration of a danger source is possible only if an operator makes an error but a dangerous influence occurs if danger is activated before the next diagnostic. Otherwise the source will be detected and neutralized during the next diagnostic.
\nIt is supposed for technologies 1 and 2 that the used diagnostic allows to provide necessary system integrity recovery after revealing danger source penetration into a system or consequences of influences. Assumption: for all time input characteristics, the probability distribution function (PDF) exists. Thus, the probability of the correct system operation within the given prognostic period (i.e., the probability of success) may be computed as a result of the use of models. For identical damage risk, to lose integrity is an addition to 1 for the probability of correct system operation, R = 1−P [3–4].
\nThere are possible next variants for technologies 1 and 2: variant 1 in the given prognostic period Treq is less than the established period between neighboring diagnostics (Treq < Tbetw. + Tdiag); variant 2 in the assigned period Treq is more than or equals to the established period between neighboring diagnostics (Treq3Tbetw. + Tdiag). Here, Tbetw. is the time between the end of the diagnostic and the beginning of the next diagnostic, Tdiag is the diagnostic time.
\nThe main output of integration modeling is the probability of the correct system operation or risk to losing system integrity during the given period of time. If probabilities for all points Тreq. from 0 to ∞ are computed, it means a trajectory of the PDF, depending on the characteristics of threats, periodic control, monitoring and recovery. And the building of PDF is the real base to prediction metrics P and R for given time Тreq.. In analogy with reliability, it is important to know a mean time between neighboring losses of integrity like mean time between neighboring failures in reliability (MTBF), but in application to quality, safety, etc.
\nFor complex systems, parallel or serial structure existing models with known PDF can be developed by usual methods of probability theory. Let’s consider the elementary structure from two independent parallel or series elements. Let PDF of time between losses of the ith element of integrity be Вi(t), that is, Вi(t) = Р (τi ≤ t); then:
\nApplying recurrently expressions (1), (2), it is possible to build PDF of time between losses of integrity for any complex system with parallel and/or series structures.
\nAll these ideas for analytical modeling operation processes are supported by the software tools “Mathematical modeling of system life cycle processes”—“know how” (registered by Rospatent №2,004,610,858), “Complex for evaluating quality of production processes” (registered by Rospatent №2,010,614,145) and others [1–4].
\nBy using the models and software tools above the problems of optimization for an element, subsystem and system can be solved through calculating probabilities of success or failure during a given period on the timeline. This approach considers the threats, conditions of counteractions and the given admissible risk established by the precedent principle. Thus, the final choice of integrated measures is allocated on a payoff to the customer in view of specificity of the created or maintained system.
\nFor example, the next general formal statements of problems for optimization can be used [6]:
\n2. On an operation stage: System parameters, technical and management measures, presented in terms of time characteristics of threats, control and/or monitoring of conditions and comprehensible recovery of lost integrity, are the most rational for the given period of operation if the minimum of risks to system integrity loss is reached at limitations on the admissible level of risk and/or probability of an admissible level of quality and expenses for operations under other operations or maintenance conditions.
\nThe combination of these formal statements also can be used in the system’s life cycle.
\nThe approach for using the developed models, methods and software tools to analyze and optimize system processes is illustrated in Figure 9.
\nThe approach to analyze and optimize system processes.
A typical set of manufacturing processes of gas preparation equipment (GPE) on the enterprise includes:
processes connected with operation of entrance threads;
processes of low temperature gas separations;
process of economical measure of gas;
processes of gas heating and reduction;
processes of candle and torch separation;
processes connected with storage and use methanol;
processes connected with storage, supply and drainage dumps of the weathered condensation and diesel fuel;
managing processes in the engineering division;
managing processes in the manufacturing division;
managing processes in booster compressor station division;
managing processes in the administrative department.
Not to tire the attentive reader, we will not state results of modeling for all processes—in examples 3 and 4, there are only results for processes connected with the operation of entrance threads and managing processes.
\nExample 3: It is required to predict the quality of the production processes and reliability of equipment connected with the operation of entrance threads.
\nInput data for modeling are formed as an analysis result of the average statistical data and requirements for production processes of the enterprise. A separate quality of each group of processes is estimated; then, quality of productions for GPE as a whole is predicted. Let an average time of recovery of each group of the processes earlier is equal to the duration of work of one shift, that is, 8 h. The predicted period is 1 month, 1 year and 5 years at observance of set modes for processes.
\nNote: For a pre-emergency condition, input data can essentially differ; that will cause also change of modeling results.
\nFor the decision the models above are used. The results of modeling of the productions connected with the operation of entrance threads are analyzed in Figure 10.
\nPrediction of quality of production processes connected with operation of entrance threads.
Results of modeling: Owing to the recovery in time technological and production processes as a result of periodic control, the mean time between failures (MTBF), affecting quality, increases from 1361 to 20,431 h, that is, by 15 times. It is reached at the expense of timely reaction in process control. The integral probability of the performing processes, connected with the operation of entrance threads with an acceptable quality, is 0.97 for a month of GPE operation, 0.70 for GPE operation in a year and 0.32 for GPE operation in 5 years. The last probability (0.32) means that it may be a real one or more accidents or failures for 5 years of GPE operation, when counter-emergency measures should be performed. Risk of this is about 0.68, that is, twice more than the probability of success.
\nAnd what about reliability? The maintenance and diagnostic measures are performed every half a year according to recommendations of equipment suppliers. How much it is effectively for real operation conditions on the level of predicted reliability?
\nResults of predicting reliability of equipment connected with the operation of entrance threads are demonstrated in Figure 11. Expected integral MTBF is equal to 5770 h. It is 3.5 times less in comparison with 20,431 h owing to daily periodic control (see the earlier section).
\nPredicted reliability of equipment connected with operation of entrance threads.
Summary: The account of daily results of control and measurements is necessary. Otherwise, if it is to be guided by only guarantee recommendations of equipment suppliers’ occurrence, at least one accident or failure demanding counter-emergency measures of protection annually is possible and for 5 years it is inevitable.
\nExample 4: The next system question is very important: What about the benefit for enterprise “the prediction of complex quality” based on the probabilistic modeling of processes? Modeling allows one to compare the quality of various productions on a uniform scale, to establish levels of acceptable quality, taking into account expenses, to allocate “bottlenecks” in each of these processes and also to develop the general and separate recommendations about process improvements. For example, the comparative results of modeling of production processes are demonstrated in Table 2.
\n№ | \nProcesses | \nProbability of providing acceptable quality during a year | \n
---|---|---|
1 | \nProcesses connected with operation of entrance threads | \n0.70–0.90 | \n
2 | \nProcesses of low temperature gas separations | \n0.62–0.87 | \n
3 | \nProcesses of economical measure of gas | \n0.9999 | \n
4 | \nProcesses of gas heating and reduction | \n0.94 | \n
5 | \nProcesses of candle and torch separation | \n0.82 | \n
6 | \nProcesses connected with storage and use methanol | \n0.63 | \n
7 | \nProcesses connected with storage, supply and drainage dumps of the weathered condensation and diesel fuel | \n0.60 | \n
8 | \nManaging processes in engineering Division, manufacturing Division, booster compressor station Division, administrative Department | \n0.67 | \n
Comparative results of production processes modeling.
Thus, with other things being equal, a more complex structure of processes, as a rule, possesses more risks. It should be considered.
\nOn the basis of the analysis of modeling results, numerous logical decisions should be made by enterprise management according to the criterion “quality-risks-cost.”
\nExample 5: There is system which consists of a 560-km pipeline for pumping liquefied natural gas across the South American territory (the source of modeling data is a technical report of one of the oil companies). All lay of the line conventionally is divided into three parts (subsystems) by service conditions: first part through the jungle (200 km), second part through the mountains (300 km) and third through the plains (60 km). These characteristics of pipeline subsystems are presented in Table 3. It is assumed that the annual profit of operation of the pipeline in the first 5 years is 1500.000 and after is 2500.000 conventional units of accounts per year. It is required to predict the risks taking into account profits and the estimated costs (in conventional units of account) for the construction and maintenance of various sections of the pipeline between 10 and 50 years of its operation.
\nCharacteristics | \nPart through the jungle (200 km) | \nPart through the mountains (300 km) | \nPart through the plains (60 km) | \n
---|---|---|---|
The frequency of potentially hazards impacts on 100 km lay of the line (technical, natural, human or criminal, etc.) | \n15 times a year | \n10 times a year | \n50 times a year | \n
The period between system controls the integrity of area | \n1 month | \n1 month | \n1 week | \n
The mean time to failure of monitoring tools at the area (without using or using existing/ prospective monitoring tools) | \n1 day/1 year | \n1 day/1 year | \n1 day/1 year | \n
The resistance of areas (the average time of preserving the integrity) for the dangerous influences statistically and in comparison with analogues | \n228.1 days | \n331.8 days | \n1217 days | \n
The average cost of construction and maintenance of the area, over 1 km, | \n1000 c.u. per year | \n2000 c.u. per year | \n200 c.u. per year | \n
Average recovery time pipeline integrity after occurrence of the fault | \n\n | 10 days | \n\n |
Characteristics of hazards, measures of control, monitoring and maintaining pipeline integrity.
Predicted risks taking into account monitoring possibilities.
As a result of applying technologies, which had been developed in 2008, the average time achievable of the safe operation is approximately 3000–5000 h. At the same mean time, failure in the jungle is 5767–8745 h; in the mountains it is 8255–12,676 h; and on the plain it is 29,500–1,22,145 h. Note that the upper estimate was inherent for the systematic maintaining of pipeline integrity (when all failures and critical areas with potential danger are identified) in the jungle and in the mountains every month and at the plains weekly. Subsystems’ state monitoring is tracked mainly in the days of control. The analysis of the results of the calculations shows that systematic monitoring allows one to increase the safety of operations of the pipeline in the jungle and in the mountains by 1.5 times, in the plain by 4 times, but throughout the 560-km stretch of the pipeline it is by 1.6 times! This is a real job for pre-emption as compared with the case of the absence of any control; when troubleshooting, it is only after the accident that cannot be overlooked. It is assumed that operative repair with restore the integrity follows after the failure detection immediately.
\nPromising technologies will implement a continuous monitoring of the pipeline at any point. For example, it may be a scan of the air situation using electronic locator fighters of the fourth and fifth generations with the smart cover. Similarly, intellectual filling of the pipeline will signal the dangers of the results with relevant coordinates and diagnosis. If we know the location and cause of the potential failure of the restoration of the integrity it becomes a routine “matter of technique.” Under these conditions there’s real will be the mean time of safe operation of the pipeline of about 165,000 h, which is achieved when the mean time of failure of monitoring tools is about a year. The mean time of failure in the jungle will be more than 28,0000 h, in the mountains more than 40,000 h and on the plains more than 18 million h (as in the engines of space vehicles!). The analysis of calculation results shows that rational frequency of periodically controlled, continuous monitoring and prompt removal of the detected faults increase security in 33–54% compared to existing technology.
\nThe results obtained show clearly the following:
\nfor the existing technologies, the security risks for 10 years constitute 0.95–0.97 (which means that a number of accidents seem almost inevitable, while in the jungles, with a probability 0.91–0.94, in the mountains with a probability 0.88–0.92 and on the plains with a probability 0.42–0.75; in 50 years, the risk exceeds 0.99 (dozens of accidents, even in the jungle, with a probability of 0.98–0.99, in the mountains with a probability of 0.97–0.98 and on the plains with a probability of 0.78–0.94);
\nfor the promising technologies, the security risks in 10 years constitute 0.35 (i.e., practically for some 10 years, we can even avoid accidents, and in the jungle, the accident will be possible with a probability of 0.24, in the mountains with a probability of 0.18 and on the plains with a probability of 0.005), in 50 years it is 0.73 (2–4 crashes in 50 years in the jungle with a probability of 0.61, in the mountains with a probability of 0.52 and in the plain with probability 0.02);
the cost will be in 10 years 8.012.000 c.u. and over 50 years it will be 40.060.000 c.u.; moreover, the costs of an area of the pipeline in the mountains are twice more than costs in the jungles and on the order more than ones on the plains;
the approximate profit of the pipeline owner costs less and without adjustment of inflation in 10 years is 11.988.000 c.u.; that in one and a half times exceeds the costs, and in 50 years is 79.940.000 c.u., which is double the costs. Moreover, the expenditure will produce returns in less than in a year. That means that when using promising technologies the quantity of accidents may be reduced on the matter; even these accidents happen either in the jungle or in the mountains. It’s quite a profitable and secure project. It must be admitted that the level of security obtained—the risks are 0.35 in 10 years and 0.73 in 50 years—can be considered as normative “acceptable.”
Thus, the examples of forecasting the security operation of the pipelines have illustrated the ability to proactively manage risk. The effectiveness is not just using the universal models but also in the justification of the necessary system requirements for new materials (pipes should be intelligent with the ability of continuous monitoring and mean time of failure for at least a year) and in technologies of restoring functional integrity, in minimizing risks on the basis of the control parameters of the processes of control, monitoring and restoring even before promising technologies have appeared! It is therefore proposed to manage the risks for pipelines of the future even before their creation and based on this, to justify the technical requirements to the system and their components.
\nThere are many standards used in the oil and gas industry (ISO 10418 “Basic surface safety systems”, ISO 13702 “Control & mitigation of fire & explosion”, ISO 14224 “Reliability/maintenance data”, ISO 15544 “Emergency response”, ISO 15663 “Life cycle costing”, ISO 17776 “Assessment of hazardous situations” etc.), but they focus on technical aspects and do not consider terrorist threats.
\nThe principal difference of GOPS consists of the fact that safety problems should be resolved in the sea because long distances from the shore and probable ice conditions in northern regions exclude any help from the outside—see Figure 13.
\nSome explanation of conditions for examples 6 and 7.
Oil and gas are usually produced on stationary stills and concrete platforms located up to 200 km from the shore at the depth from several dozens to several hundred meters. There are nearly 5000 sea platforms dispersed all over the world. Dozens of thousand oil wells are drilled from these platforms. Produced oil is delivered to the buyers by tankers or directly through pipelines.
\nProduced gas before transportation goes into the liquefied natural gas terminals. After the liquefaction its volume reduces by 600 times, that makes its transportation profitable. Statistics shows that during the time of sea field development, emergencies are distributed in the following ways [10, 11]: drilling—32% (including 23% at survey and 9% at production drilling); gas-and-oil production—19%; ship collision and towing of floating drilling rigs and blocks for platform construction —14%; storms—11%; floating drilling rigs’ delivery to the point of drilling—6%; and other kinds of works—18%.
\nA safety policy concerning sea GOPS safety includes accident prevention and drawing, plans concerning failure consequences of liquidation and actions taken in case of emergencies. Special brigades are formed and trained to prevent and liquidate failure consequences. High-quality materials and pipes and application of computer diagnostics for pipe integrity monitoring provide safety of the GOPS operation.
\nAll safety measures undertaken nowadays provide system protection from inexperienced personnel (because according to statistics about 80% of all failures are connected with the human factor) or from the natural causes and “cataclysms” which are of an unpremeditated character. However, the attitude to safety cardinally varies in case of terrorist threats because terrorist actions are malicious and aimed at damaging the system through its vulnerable “bottlenecks.” As a result the existing risks of system safety violation essentially grow.
\nThe examples 6 and 7 are devoted to modeling processes of possible terrorist influence and GOPS safety provision (including platforms, coastal technological complexes including terminals for floating storage and offloading, liquefied natural gas terminals, pipelines, tubing stations) and to withdraw quantitative evaluations of their vulnerability in various scenarios.
\nExample 6: connected with an estimation of effectiveness of a safety monitoring system for sea GOPS. Before we start the analysis of possible terrorist threats, let us consider the basic dangers that can arise on sea platforms in case of failures. They are explosions of fuel-air mixed clouds; generation and burning of fire balls; oil spill and burning; separation and spread of technological equipment parts; and others. Each of these dangers can aggravate consequences of failures, that is, lead to the “dominoes” effect. To control risks the following measures are taken: application of safe technologies; measures preventing dangerous situations; applications of systems providing early detection of emergencies; control over operating parameters of the technological process, the signal system and the notification about emergency technologies; measures directed on mitigation of emergency consequences; and preparation of a platform staff to react immediately.
\nThe analysis shows that the basic preventive mechanism of risk reduction is safety monitoring in various variations of its application. Let us estimate commonly used safety technologies, technology 1 (periodical diagnostics of system integrity without the continuous monitoring between diagnostics) and technology 2 (continuous monitoring between periodical diagnostics is added to technology 1)—see Section 4.
\nLet’s estimate efficiency of sea GOPS safety technologies used in the case of emergencies for dozens of years. Thus we take into account that the basis of safety systems consists of automatic facilities’ mean time where failures of which are estimated for several years.
\nTo form inputs for probabilistic modeling the arising of basic dangers for sea platforms in the case of failures is considered. There is generally one of the abovementioned technologies to provide safety of GOPS components (platforms, coastal technological complexes including floating storage and offloading terminals, liquefied natural gas terminals, pipelines, tubing stations). The script of emergency development provides frequency of danger source appearance equal to 1 time per 24 h with a mean time of activization within an hour. Time between the termination of the previous and the beginning of the next diagnostics taking into account broken integrity recovery is 2 h. Let us suppose that monitoring is performed by automatic means of tracking the integrity of system components. To such means systems of fire and gas detection, systems of water fire-fighting and foaming, circled fire mains, systems of platform irrigation, pressure relief systems, emergency switching of systems, various locking device and so on may be related. Let the mean time between failures of these means be not less than 2 years.
\nIt needs to estimate a safety of the sea platform operation in such scenarios within several hours, a day, several weeks and a month.
\nThe integrated results of calculations prove that at the realization of technology 1, the required safety is provided only for several working days—what is inadmissible in practice. If the most effective technology, technology 2 is realized, the probability that a dangerous influence does not occur within 24 h is above 0.99997, that is, the probability of emergency is about 0.00003. At the same time provision of the required safety within a month in conditions of daily failure danger this risk increases up to 0.001 that also appears to be a practically admissible result.
\nAgainst the background of proved measures of counteraction to sources of emergency the situation concerning the struggle against terrorist threats appears to be cardinally worse because this problem is still at the initial stage. Other things being equal, let us estimate the expected sea GOPS protection from terrorist threats with differences in abilities of a security service operator to reveal suspicious actions and objects which can be a means of terrorist purpose realization.
\nExample 7: Let the deliberately formal conditions of a terrorist influence scenario be similar to the emergency dangers in example 6. Let us suppose that for providing protection of sea GOPS platforms from terrorist threats, any of the protecting technologies are used. Let the scenario of the potentially dangerous influence of terrorists provide the frequency of a danger source appearance from air, the water table or from under water equal to 1 time per 24 h with the mean time of activation after penetration onto a platform equal to 1 h. The time between the termination of the previous and the beginning of the following diagnostics taking into consideration the broken integrity recovery is 2 h. Let us assume the mean continuous time of potentially faultless operators’ works in each shift to be 6 hours. It is required to evaluate the safety of the sea platform operation in such scenarios within several hours, days, weeks and a month.
\nThe integrated calculation results prove that without any additional protection the system remains in safety with the probability of 0.9 only within 2–3 h. It is explained by a comparative rarity of a danger source appearance. If the 1st technology of counteraction to terrorists is applied (this technology exists on the most of platforms and implies visual tracking of air conditions, the working hours what is an equivalent to the case if there are no measures of counteraction to terrorists on a platform at all.
\nIf the most effective second technology is used, the probability of GOPS integrity within a day is more than 0.92, that is, the risks of latent introductions of a terrorist danger source into a system and the overcoming of all technological protection barriers preventing realization of terrorist threats in the conceived volume approximate to 0.08. At the same time to provide the required safety within a month in conditions of daily danger that a sudden terrorist attack happens, this risk runs up to 0.93. The main cause of this is insufficient preparedness of operators to recognize terrorist threats at the background of other technical threats. That’s why it is necessary to increase the mean time between failures of a safety service operator to tens and hundreds of hours what requires creation of special “smart subsystems” in order to support operator functions (radar-tracking, optical, acoustic, electromagnetic means etc.). Compare the results of examples 6 and 7.
\nThe presented probabilistic approaches allow us to research different problems for providing safe and effective development of hydrocarbon deposits and rational operation of oil and gas systems. Their application in the system’s life cycle helps to answer the main question. “What rational measures should lead to expected effects without wasted expenses, when, by which controllable and uncontrollable conditions and costs?” The efficiency from implementation is commensurable with expenses for system creation.
\nThe probabilistic modeling, comprehensive and systematic studies on the competitiveness of OGS have been carried out in Gubkin Russian State University of Oil and Gas (National Research University) over a period of several years. These researches are concerned with the most important economic branch, which largely determines the country’s energy security and efficiency. Certainly, the integral view of OGS competitiveness seamlessly includes the most important components of quality, safety, energy efficiency, environmental compatibility, economic aspects and so on. In turn, these components are also complex, integral and affect a wide range of activities. Moreover, competitiveness as a complex integral metric characterizes the studied considered systems and objects (as living organisms) that have the property of changes in the life time. The proposed probabilistic models and methods are widely used in the practice of education and research.
\nOsteoporosis is a metabolic disorder causing bone mineral density to decrease and changing the bone structure [1]. It is a degenerative disease whose initial symptoms are not known with certainty. Someone who suffers from osteoporosis will usually experience complaints if the stage is severe [2, 3]. Bones with osteoporosis will experience a decrease in mechanical strength so they are prone to fracture, and will easily crack or become brittle if exposed to a hard object. It is characterized by low bone mass and structural breakdown of bone tissue. Some parts of the body that are at risk for osteoporosis include the spine, pelvis, femur, tibia, pelvic bones, wrist bones, and other bone parts dominated by the trabecular bone [4, 5, 6].
Osteoporosis can be diagnosed clinically using bone mineral density measurements. At present, bone densitometry is the standard method for diagnosis and treatment monitoring. However, it still possesses significant drawbacks because it cannot give information about the structural manifestations of the disease. Frequently, bone mineral density is analyzed using x-ray or ultrasound imaging methods. In x-ray imaging such as dual-energy x-ray absorptiometry (DEXA) and quantitative computer tomography (QCT), the intensity of the image is correlated to the mineral density of the tissue. In ultrasound, the intensity of the image reflects changes in the frequency and amplitude of sound waves traveling through tissue. X-ray procedures employ ionizing radiation, which can have a damaging impact in sufficient doses. Ultrasound, although harmless, offers only a small field of view, which can restrict measurement accuracy. In addition to bone density, bone quality which includes bone microarchitecture is also a concern. Recent developments in imaging, especially electron microscopy, can now give detailed information about the effects of architecture on disease progression and regression in response to treatment. However, before the diagnosis is made, of course, it is necessary to study and research in a sample or biological material to determine the process of bone remodeling and osteoporosis. The samples analyzed generally use rats as animal models. It takes a long time to make rats osteoporosis naturally. Therefore, rats were given treatment to condition the occurrence of osteoporosis. Some of the common actions taken to condition osteoporosis rats are by giving them a calcium-deficient diet or by performing ovariectomy on these rats [7, 8, 9, 10].
Several characterization tools that can be used to analyze mouse bones include X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Ultraviolet (UV)-visible Spectroscopy, or Atomic Absorption Spectroscopy (AAS). However, these tools provide information in the form of numbers or graphs. A promising imaging modality for morphological analysis of both cortical and trabecular bone is electron microscopy. The types of electron microscopes commonly used to analyze bone morphology are Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM). Figure 1 illustrates the different imaging modalities, between SEM and TEM, which were used to analyze the morphology of the rat femur bone.
(a) Image of SEM scans, and (b) image of TEM scans on femoral bones of rats from the osteoporosis group.
This review focuses on the emerging methodology of quantitative electron microscopy to assess the bone structure and morphology of osteoporotic rats. For more than 10 years, numerous approaches have been investigated to obtain quantitative image-based information on bone architecture, both trabecular bone, and cortical bone. An indirect method that does not require resolution at individual trabecular scales and can therefore be performed at any skeletal location, a recoverable component of the degree of total transverse relaxation. Therefore, electron microscopy-based structure analysis is technically demanding in terms of the required image acquisition. Other requirements that must be fulfilled involve motion correction and image registration, both of which are important to achieve the reproducibility required in repeated studies. The main targeted clinical application involves the prediction of fracture risk in femoral rats conditioned by osteoporosis due to ovariectomy.
An electron microscope is a type of microscope in which the illumination source is an electron beam. Illumination itself is a process of light coming to an object. There are electron microscopes that have high image resolution, even magnifying objects on the nanometer scale, which are produced by the controlled use of electrons in a vacuum captured on a fluorescent screen. The first electron microscope was introduced by an engineer and professor from German, Ernst Ruska (1906-1988), in 1931, and the same principles behind his prototype still dominate modern Ems [11, 12].
Electron microscopy uses signals generated by the interaction of the electron beam with the sample to gain information about its structure, morphology, and composition. The process and major parts of an electron microscope are:
Electrons are produced by the electron gun
The electron beam is concentrated on the sample by condenser lenses.
About 100 kV – 1000 kV accelerating voltage is employed between the tungsten filament and anode to move electrons down the column.
The sample to be observed should be fabricated very thin, or minimal 200 times thinner than that observed in optical microscopes. A very thin sample with a size of 20-100 nm was sliced and put in the sample holder.
The electronic beam traverses the sample and electrons are scattered relying on the thickness or refractive index of different areas of the sample.
The denser sample areas will scatter more electrons so that the image displayed in these areas will be darker because fewer electrons hit this area of the screen. Contrarily, the transparent areas will look brighter.
The electron beam leaving the sample is transferred to the objective lens which will make a magnified image.
The eyepiece then renders the final image for further magnification.
Electron microscopes are categorized into three types based on operating styles:
Nowadays, scanning electron microscopy (SEM) is a robust and effective imaging instrument. It is employed for scanning surfaces with a magnification from 1 m to 1 nm which depends on the hardware used to create the electron beam with various lenses and vacuum systems. Further, it is integrated with an energy dispersion spectrometer to combine the elemental analysis potential on the sample surface. SEM imaging has new characteristics those are backscattering electrons and secondary electrons which increase the scanning potential. The electron gun includes the main parts of the SEM components. With the existence of different magnetic lenses and vacuum systems, SEM has become a unique imaging tool [13].
The characterization method with SEM can deliver visual information on the morphology of the bone surface. SEM images can also be analyzed with an image processing program such as ImageJ, with the output in the form of a histogram of pixels that can provide information about the cavities in the bone and their distribution. From the histogram, bone quality can be known quantitatively by looking at the average pixel value and the percentage of cavity intensity. Schematically, the scan with SEM is shown in Figure 2.
Schematic flow diagram of a scanning electron microscope [
From Figure 2, Electron Microscopes utilize electrons beam to illuminate a sample and construct an image with high magnification. The electrons from the electron source passing through the condenser lenses, aperture, scanning coil, objective lens, detectors and hit the gold-coated sample positioned on its holder. The condenser lenses center the electron beam in a specific area corresponding to the sample and thus generate the image. Electrons hit the sample surface thereby producing the secondary electrons which are detected by the secondary electron detector and transformed into a signal delivered to a monitor scanner.
Conventional SEM relies on the emanation of auxiliary electrons from the sample surface. As its large focus depth, the SEM is the EM analog of the stereo light microscope. It gives nitty-gritty pictures of the cell surface and the whole life form. It can moreover be worked for molecule checking and measuring, and for handle control. A SEM, it is so called, because it forms the image by scanning a focused electron beam onto the sample surface in a raster design. The primary electron beam interacting with atoms nearby the surface induces particle emission at any location in the raster. The emissions, for instance, include low energy secondary electrons, high energy scattering electrons, X-rays, and photons that then can be gathered by distinct detectors, and their relative quantities are converted to brightness at every equivalence point on the cathode ray tube (CRT). Due to the considerably smaller raster size than the CRT screen display, the resulting image is the image magnification of the sample. SEMs are equipped with proper equipment such as secondary detectors, backscattering, and X-rays, which can be functioned to analyze the topography and atomic composition of the sample and the surface distribution of immune labels [15, 16].
Transmission electron microscopes are exploited to examine thin samples (parts of tissue, molecules, etc.) that electrons can traverse to produce a projected image. TEM is analogous to a conventional light microscope. Schematically, the scan with TEM is presented in Figure 3.
Schematic flow diagram of a transmission electron microscope [
In Figure 3, the TEM applies high-energy electrons for imaging. It has been developed since the 1938’s. Its operation requires a very high voltage of about 500 − 1000 kV with a resolution reaching 0.1 nm. During TEM operation, the electrons beam is generated and transmitted through an ultra-thin sample. Then, the unscattered electrons are transmitted through the sample and hit the fluorescent screen at the bottom of the microscope, thus producing an image. By changing the gun voltage, the electron velocity can be modified which in turn changes the image. Commonly, TEM generates a grayscale image that exhibits lighter and darker regions. The lighter regions demonstrate regions with a large number of transmitted electrons while the darker ones represent a lower number and denser regions in the sample. The sample used in TEM should be prepared thin enough for electrons to be transmitted [17].
Another type of development of the electron microscope is the reflection electron microscope (REM). The REM is an electron microscope that has almost the same way of working as TEM, the difference is that REM uses the detection of electron reflections on the object’s surface. The sample is semi-infinite and the surface to be observed is almost parallel to the electron beam. The transmitted spot may or may not be observable, depending on the sample size as shown in Figure 4. This technique is specifically used in combination with the Reflection High Energy Electron Diffraction (RHEED) technique and the reflection high-energy loss spectrum (RHELS) technique.
These ray diagrams illustrate (a) TEM and (b) REM [
REM could be a combination of imaging, diffraction, and spectroscopy procedures for the characterization of topography, crystal structure, and composition of surfaces of single crystals. High-energy electrons are occurring at looking points to the surface and reflected electrons are utilized to create a REM picture. Utilization of REM in analyzing osteoporosis in bone is still rarely done, because REM has several drawbacks including REM images are shortened in the direction of electron events and high resolution is only achieved in the normal direction, so that in analyzing surface topographic details more than one azimuth is needed. Meanwhile, bones that are not homogeneous can produce different images in each image. These techniques are applicable to metal [19], semiconductor [20], crystal surfaces [21], surface reconstructions and phase transformations [22], correlation between topographical features and reconstructions, directions, distribution, and motion of surface steps, dislocations on surfaces, nucleation and growth of films, and surface reactions [23].
Sample preparation for REM is the same as for other types of electron microscopy, i.e., it must be ensured that the surface is sufficiently flat and clean. The size of the sample should fit the microscope sample holder by about 3 mm. Then inserted into the electron microscope with a surface normal perpendicular to the optical axis. The nominal size of a REM sample is no more than 1 mm3 for a sample holder which gets 3 mm grids. Hence, the perceptible surface is about 1 mm or less. The lower restrain of the surface area is approximately 10 μm in diameter.
Electron microscopy (EM) is a method to obtain biological and non-biological samples’ images with a high-resolution. This method is frequently employed in biomedical research to examine the detailed structure of tissues, cells, organelles, and macromolecular complexes. High-resolution EM images are produced from the use of electrons having very short wavelengths as the illumination source. EM is used in conjunction with numerous additional methods (e.g., thin cutting, immune labeling, and negative staining) to answer specific questions. EM images can deliver crucial information about the structural and morphological basis of bone. Several results of prior studies that investigated bone with EM are presented in Table 1.
Scanning electron microscopy (SEM) | Transmission electron microscopy (TEM) |
---|---|
The SEM image exhibited that the longer the time after ovariectomy, the greater the degree of damage seen in the tibial cavity [24] | Acicular crystals of apatite with approximate dimensions of ~20–30 nm by 5 nm. Gap zones and overlap zones in collagen fibrils [25] |
The group of ovariectomized rats had histograms that increasingly shifted more black areas. Areas that were black or dark relate to cavities in the bone [26] | Apatite crystals that resemble tablet form, in the control group have a longer size, and for groups of ovariectomized rats there was a decrease in size both length and width [26] |
Network organization in trabecular bone showing topographical details [27] | Different calcium phosphate minerals morphologies in the bone extracellular matrix: dense granules, globular aggregates of needle-like apatite, and mature fibrous minerals [28, 29] |
Canalicular network with residing osteocytes [30] | Woven arrangement of aligned collagen fibrils in the ordered phase of trabecular bone [31] |
Osteons and cement line delineating osteonal and interstitial bones [32] | Characteristic collagen banding pattern with a periodicity of ~67 nm [33] |
Cross-sectional photomontage of an entire human rib bone [34] | Disorganized, entangled collagen fibrils without characteristic banding pattern in the disordered phase of trabecular bone [35] |
Characterization of bone structure by SEM and TEM.
After obtaining the image from the electron microscope, the next step that needs to be done is to analyze the resulting image. Several applications that can be used to process the output image of an electron microscope, including ImageJ, Matlab, Python, OpenCV, Dragonfly, HyperSpy, and others. Each has its own advantages and disadvantages. However, on this occasion, we will review the analysis of images from electron microscopy using the ImageJ application. Figure 5 shows the results of SEM imaging of sham rat femur (a) and osteoporosis due to ovariectomy (b).
SEM image of the rat femur bone, (a) sham, (b) osteoporosis due to ovariectomy.
Figure 5 shows an SEM image of a rat bone taken from the femur at 1000x magnification. Figure 5(a) SEM image of the femur bone of a 13-week-old sham rat, visually it can be seen that the surface is denser, there are no large cavities found. This is different from the SEM image shown in Figure 5(b), the image was taken from the femur bones of rats with osteoporosis due to ovariectomy treatment. The surface is clearly visible in the presence of wider cavities. The picture was taken when the rats were 21 weeks old or 9 weeks after being given ovariectomy. In accordance with the results of previous studies, the rats began to show the characteristics of osteoporosis at the ninth week since ovariectomy [36].
The characteristics of osteoporosis are clearer from the SEM image that has been analyzed with the help of the ImageJ application as shown in Figure 6. Figure 6(a) results of the analysis of the sham femur, the black color is thicker and fused together, indicating that the bone is still solid. This is supported by the results of the [37] study which showed that the bones of sham rats contained minerals such as calcium, magnesium, and phosphorus which were still normal. Meanwhile in Figure 6(b) the results of the analysis of the femur bones of rats treated with ovariectomy, it appears that the color is lighter, with the black parts that have started to break off and are thinner. This is because ovariectomy treatment can cause a decrease in the hormone estrogen in the body. With a decrease in the hormone estrogen, bone resorption by osteoclasts increases, and conversely osteoblast activity becomes inhibited [38, 39, 40]. As a result, bone density will also decrease, and osteoporosis occurs [41]. In addition, a decrease in the hormone estrogen can also increase the resorption of calcium (Ca) in bone, so that bone mass will decrease [42, 43]. Even the absorption of Ca in the intestine also decreases and the excretion of Ca through the kidneys increases [44, 45, 46]. All these conditions cause parathyroid hormone activity to increase and bone density to decrease which in turn triggers osteoporosis [47, 48].
SEM image of rat femur after analysis with ImageJ, (a) sham, (b) osteoporosis due to ovariectomy.
Quantitatively several parameters that can be known from SEM image analysis with Image J application include particle diameter, percentage of voids, or porosity analysis. Particle diameter analysis for the same sample as previously mentioned is shown in Figure 7. Figure 7(a) shows the particle diameter size of the sham rat femur bone ranging from 1.5 to 34.4 μm. The particle diameter experienced a significant increase in the ovariectomized femur bone, the highest size reaching 150.2 μm as shown in Figure 7(b). Larger particle sizes tend to be more porous, as a result, are more brittle [49].
Particle diameter size of the rat femur bone, (a) sham, (b) osteoporosis due to ovariectomy.
Likewise, TEM images can be analyzed and obtained the same information as for images from SEM. The output of the porosity analysis can also be carried out, some quantitative data can be obtained from the results of the porosity analysis, namely the pore volume and the percentage of pores. Some of these parameters can be used as a reference for osteoporosis analysis in bone, especially in experimental animal models.
Imaging at the nanoscale is very important to analyze the quality and structure of bone morphology. This review examines the images produced by electron microscopy of the femur bones of rats under sham conditions and osteoporosis due to ovariectomy. The scanned electron microscopy image with the help of the ImageJ application provides information that the femur bones of ovariectomized rats show signs of osteoporosis. Some of the parameters that characterize the cavities in the ovariectomized femur appear wider, with the edges of the cavity appearing to be cracked. In addition, the particle diameter also increased by an average of 77.16%. Therefore, electron microscopy is one of the best approaches, which can directly provide ultrastructural evidence for bone morphology, and furthermore, the results of this bone morphology analysis can provide insight into the characteristics and processes of osteoporosis.
This project was partially funded by “Hibah TADOK Universitas Indonesia” No. 1331/UN2.R3.1/HKP.05.00/2018.
The author declares no conflict of interest.
AAS | Atomic Absorption Spectroscopy |
DEXA | Dual-Energy X-Ray Absorptiometry |
EM | Electron Microscopy |
FTIR | Fourier Transform Infrared Spectroscopy |
QCT | Quantitative Computer Tomography |
REM | Reflection Electron Microscope |
SEM | Scanning Electron Microscope |
TEM | Transmission Electron Microscope |
UV | Ultraviolet |
XRD | X-Ray Diffraction |
Units | |
kV | Kilovolt |
nm | Nanometer |
μm | Micrometer |
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The forearc basin type and tectonic history are characteristic for each forearc territory, reflecting the differences in plate tectonic processes. Several major unconformity events seem to be synchronous for a forearc territory or whole forearc territories around Japan, suggesting that these events originated from more or less wider scale plate tectonic events. In the NE Japan forearc territory, the Oligocene unconformity can be the largest events, which transformed the forearc basin styles from the trench slope break-uplifted, fluvial system-dominated type to the tensional, deeper marine sloped type. In the SW Japan and Ryukyu forearc territories, the latest Oligocene to Middle Miocene gap was the transformation phase from the Palaeogene Shimanto-type forearc and accretionary complex, to the Neogene compressive, sloped to ridged forearc basins, developments of which have been interrupted by several unconformity events possibly related to changes in plate tectonic condition. 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She is also a UNESCO-trained International Bioethics Facilitator.",institutionString:"University of the Witwatersrand",institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419588",title:"Ph.D.",name:"Sergio",middleName:"Alexandre",surname:"Gehrke",slug:"sergio-gehrke",fullName:"Sergio Gehrke",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0033Y000038WgMKQA0/Profile_Picture_2022-06-02T11:44:20.jpg",biography:"Dr. Sergio Alexandre Gehrke is a doctorate holder in two fields. The first is a Ph.D. in Cellular and Molecular Biology from the Pontificia Catholic University, Porto Alegre, Brazil, in 2010 and the other is an International Ph.D. in Bioengineering from the Universidad Miguel Hernandez, Elche/Alicante, Spain, obtained in 2020. In 2018, he completed a postdoctoral fellowship in Materials Engineering in the NUCLEMAT of the Pontificia Catholic University, Porto Alegre, Brazil. He is currently the Director of the Postgraduate Program in Implantology of the Bioface/UCAM/PgO (Montevideo, Uruguay), Director of the Cathedra of Biotechnology of the Catholic University of Murcia (Murcia, Spain), an Extraordinary Full Professor of the Catholic University of Murcia (Murcia, Spain) as well as the Director of the private center of research Biotecnos – Technology and Science (Montevideo, Uruguay). Applied biomaterials, cellular and molecular biology, and dental implants are among his research interests. He has published several original papers in renowned journals. In addition, he is also a Collaborating Professor in several Postgraduate programs at different universities all over the world.",institutionString:null,institution:{name:"Universidad Católica San Antonio de Murcia",country:{name:"Spain"}}},{id:"342152",title:"Dr.",name:"Santo",middleName:null,surname:"Grace Umesh",slug:"santo-grace-umesh",fullName:"Santo Grace Umesh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/342152/images/16311_n.jpg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"333647",title:"Dr.",name:"Shreya",middleName:null,surname:"Kishore",slug:"shreya-kishore",fullName:"Shreya Kishore",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/333647/images/14701_n.jpg",biography:"Dr. Shreya Kishore completed her Bachelor in Dental Surgery in Chettinad Dental College and Research Institute, Chennai, and her Master of Dental Surgery (Orthodontics) in Saveetha Dental College, Chennai. She is also Invisalign certified. She’s working as a Senior Lecturer in the Department of Orthodontics, SRM Dental College since November 2019. She is actively involved in teaching orthodontics to the undergraduates and the postgraduates. Her clinical research topics include new orthodontic brackets, fixed appliances and TADs. She’s published 4 articles in well renowned indexed journals and has a published patency of her own. Her private practice is currently limited to orthodontics and works as a consultant in various clinics.",institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"323731",title:"Prof.",name:"Deepak M.",middleName:"Macchindra",surname:"Vikhe",slug:"deepak-m.-vikhe",fullName:"Deepak M. Vikhe",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/323731/images/13613_n.jpg",biography:"Dr Deepak M.Vikhe .\n\n\t\n\tDr Deepak M.Vikhe , completed his Masters & PhD in Prosthodontics from Rural Dental College, Loni securing third rank in the Pravara Institute of Medical Sciences Deemed University. He was awarded Dr.G.C.DAS Memorial Award for Research on Implants at 39th IPS conference Dubai (U A E).He has two patents under his name. He has received Dr.Saraswati medal award for best research for implant study in 2017.He has received Fully funded scholarship to Spain ,university of Santiago de Compostela. He has completed fellowship in Implantlogy from Noble Biocare. \nHe has attended various conferences and CDE programmes and has national publications to his credit. His field of interest is in Implant supported prosthesis. Presently he is working as a associate professor in the Dept of Prosthodontics, Rural Dental College, Loni and maintains a successful private practice specialising in Implantology at Rahata.\n\nEmail: drdeepak_mvikhe@yahoo.com..................",institutionString:null,institution:{name:"Pravara Institute of Medical Sciences",country:{name:"India"}}},{id:"204110",title:"Dr.",name:"Ahmed A.",middleName:null,surname:"Madfa",slug:"ahmed-a.-madfa",fullName:"Ahmed A. Madfa",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204110/images/system/204110.jpg",biography:"Dr. Madfa is currently Associate Professor of Endodontics at Thamar University and a visiting lecturer at Sana'a University and University of Sciences and Technology. He has more than 6 years of experience in teaching. His research interests include root canal morphology, functionally graded concept, dental biomaterials, epidemiology and dental education, biomimetic restoration, finite element analysis and endodontic regeneration. Dr. Madfa has numerous international publications, full articles, two patents, a book and a book chapter. Furthermore, he won 14 international scientific awards. Furthermore, he is involved in many academic activities ranging from editorial board member, reviewer for many international journals and postgraduate students' supervisor. Besides, I deliver many courses and training workshops at various scientific events. Dr. Madfa also regularly attends international conferences and holds administrative positions (Deputy Dean of the Faculty for Students’ & Academic Affairs and Deputy Head of Research Unit).",institutionString:"Thamar University",institution:null},{id:"210472",title:"Dr.",name:"Nermin",middleName:"Mohammed Ahmed",surname:"Yussif",slug:"nermin-yussif",fullName:"Nermin Yussif",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/210472/images/system/210472.jpg",biography:"Dr. Nermin Mohammed Ahmed Yussif is working at the Faculty of dentistry, University for October university for modern sciences and arts (MSA). Her areas of expertise include: periodontology, dental laserology, oral implantology, periodontal plastic surgeries, oral mesotherapy, nutrition, dental pharmacology. She is an editor and reviewer in numerous international journals.",institutionString:"MSA University",institution:null},{id:"204606",title:"Dr.",name:"Serdar",middleName:null,surname:"Gözler",slug:"serdar-gozler",fullName:"Serdar Gözler",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/204606/images/system/204606.jpeg",biography:"Dr. Serdar Gözler has completed his undergraduate studies at the Marmara University Faculty of Dentistry in 1978, followed by an assistantship in the Prosthesis Department of Dicle University Faculty of Dentistry. Starting his PhD work on non-resilient overdentures with Assoc. Prof. Hüsnü Yavuzyılmaz, he continued his studies with Prof. Dr. Gürbüz Öztürk of Istanbul University Faculty of Dentistry Department of Prosthodontics, this time on Gnatology. He attended training programs on occlusion, neurology, neurophysiology, EMG, radiology and biostatistics. In 1982, he presented his PhD thesis \\Gerber and Lauritzen Occlusion Analysis Techniques: Diagnosis Values,\\ at Istanbul University School of Dentistry, Department of Prosthodontics. As he was also working with Prof. Senih Çalıkkocaoğlu on The Physiology of Chewing at the same time, Gözler has written a chapter in Çalıkkocaoğlu\\'s book \\Complete Prostheses\\ entitled \\The Place of Neuromuscular Mechanism in Prosthetic Dentistry.\\ The book was published five times since by the Istanbul University Publications. Having presented in various conferences about occlusion analysis until 1998, Dr. Gözler has also decided to use the T-Scan II occlusion analysis method. Having been personally trained by Dr. Robert Kerstein on this method, Dr. Gözler has been lecturing on the T-Scan Occlusion Analysis Method in conferences both in Turkey and abroad. Dr. Gözler has various articles and presentations on Digital Occlusion Analysis methods. He is now Head of the TMD Clinic at Prosthodontic Department of Faculty of Dentistry , Istanbul Aydın University , Turkey.",institutionString:"Istanbul Aydin University",institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"240870",title:"Ph.D.",name:"Alaa Eddin Omar",middleName:null,surname:"Al Ostwani",slug:"alaa-eddin-omar-al-ostwani",fullName:"Alaa Eddin Omar Al Ostwani",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/240870/images/system/240870.jpeg",biography:"Dr. Al Ostwani Alaa Eddin Omar received his Master in dentistry from Damascus University in 2010, and his Ph.D. in Pediatric Dentistry from Damascus University in 2014. Dr. Al Ostwani is an assistant professor and faculty member at IUST University since 2014. \nDuring his academic experience, he has received several awards including the scientific research award from the Union of Arab Universities, the Syrian gold medal and the international gold medal for invention and creativity. Dr. Al Ostwani is a Member of the International Association of Dental Traumatology and the Syrian Society for Research and Preventive Dentistry since 2017. He is also a Member of the Reviewer Board of International Journal of Dental Medicine (IJDM), and the Indian Journal of Conservative and Endodontics since 2016.",institutionString:"International University for Science and Technology.",institution:{name:"Islamic University of Science and Technology",country:{name:"India"}}},{id:"42847",title:"Dr.",name:"Belma",middleName:null,surname:"Işik Aslan",slug:"belma-isik-aslan",fullName:"Belma Işik Aslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/42847/images/system/42847.jpg",biography:"Dr. Belma IşIk Aslan was born in 1976 in Ankara-TURKEY. After graduating from TED Ankara College in 1994, she attended to Gazi University, Faculty of Dentistry in Ankara. She completed her PhD in orthodontic education at Gazi University between 1999-2005. Dr. Işık Aslan stayed at the Providence Hospital Craniofacial Institude and Reconstructive Surgery in Michigan, USA for three months as an observer. She worked as a specialist doctor at Gazi University, Dentistry Faculty, Department of Orthodontics between 2005-2014. She was appointed as associate professor in January, 2014 and as professor in 2021. Dr. Işık Aslan still works as an instructor at the same faculty. She has published a total of 35 articles, 10 book chapters, 39 conference proceedings both internationally and nationally. Also she was the academic editor of the international book 'Current Advances in Orthodontics'. She is a member of the Turkish Orthodontic Society and Turkish Cleft Lip and Palate Society. She is married and has 2 children. Her knowledge of English is at an advanced level.",institutionString:"Gazi University Dentistry Faculty Department of Orthodontics",institution:null},{id:"178412",title:"Associate Prof.",name:"Guhan",middleName:null,surname:"Dergin",slug:"guhan-dergin",fullName:"Guhan Dergin",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178412/images/6954_n.jpg",biography:"Assoc. Prof. Dr. Gühan Dergin was born in 1973 in Izmit. He graduated from Marmara University Faculty of Dentistry in 1999. He completed his specialty of OMFS surgery in Marmara University Faculty of Dentistry and obtained his PhD degree in 2006. In 2005, he was invited as a visiting doctor in the Oral and Maxillofacial Surgery Department of the University of North Carolina, USA, where he went on a scholarship. Dr. Dergin still continues his academic career as an associate professor in Marmara University Faculty of Dentistry. He has many articles in international and national scientific journals and chapters in books.",institutionString:null,institution:{name:"Marmara University",country:{name:"Turkey"}}},{id:"178414",title:"Prof.",name:"Yusuf",middleName:null,surname:"Emes",slug:"yusuf-emes",fullName:"Yusuf Emes",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178414/images/6953_n.jpg",biography:"Born in Istanbul in 1974, Dr. Emes graduated from Istanbul University Faculty of Dentistry in 1997 and completed his PhD degree in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery in 2005. He has papers published in international and national scientific journals, including research articles on implantology, oroantral fistulas, odontogenic cysts, and temporomandibular disorders. Dr. Emes is currently working as a full-time academic staff in Istanbul University faculty of Dentistry Department of Oral and Maxillofacial Surgery.",institutionString:null,institution:{name:"Istanbul University",country:{name:"Turkey"}}},{id:"192229",title:"Ph.D.",name:"Ana Luiza",middleName:null,surname:"De Carvalho Felippini",slug:"ana-luiza-de-carvalho-felippini",fullName:"Ana Luiza De Carvalho Felippini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192229/images/system/192229.jpg",biography:null,institutionString:"University of São Paulo",institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"256851",title:"Prof.",name:"Ayşe",middleName:null,surname:"Gülşen",slug:"ayse-gulsen",fullName:"Ayşe Gülşen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256851/images/9696_n.jpg",biography:"Dr. Ayşe Gülşen graduated in 1990 from Faculty of Dentistry, University of Ankara and did a postgraduate program at University of Gazi. \nShe worked as an observer and research assistant in Craniofacial Surgery Departments in New York, Providence Hospital in Michigan and Chang Gung Memorial Hospital in Taiwan. \nShe works as Craniofacial Orthodontist in Department of Aesthetic, Plastic and Reconstructive Surgery, Faculty of Medicine, University of Gazi, Ankara Turkey since 2004.",institutionString:"Univeristy of Gazi",institution:null},{id:"255366",title:"Prof.",name:"Tosun",middleName:null,surname:"Tosun",slug:"tosun-tosun",fullName:"Tosun Tosun",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255366/images/7347_n.jpg",biography:"Graduated at the Faculty of Dentistry, University of Istanbul, Turkey in 1989;\nVisitor Assistant at the University of Padua, Italy and Branemark Osseointegration Center of Treviso, Italy between 1993-94;\nPhD thesis on oral implantology in University of Istanbul and was awarded the academic title “Dr.med.dent.”, 1997;\nHe was awarded the academic title “Doç.Dr.” (Associated Professor) in 2003;\nProficiency in Botulinum Toxin Applications, Reading-UK in 2009;\nMastership, RWTH Certificate in Laser Therapy in Dentistry, AALZ-Aachen University, Germany 2009-11;\nMaster of Science (MSc) in Laser Dentistry, University of Genoa, Italy 2013-14.\n\nDr.Tosun worked as Research Assistant in the Department of Oral Implantology, Faculty of Dentistry, University of Istanbul between 1990-2002. \nHe worked part-time as Consultant surgeon in Harvard Medical International Hospitals and John Hopkins Medicine, Istanbul between years 2007-09.\u2028He was contract Professor in the Department of Surgical and Diagnostic Sciences (DI.S.C.), Medical School, University of Genova, Italy between years 2011-16. \nSince 2015 he is visiting Professor at Medical School, University of Plovdiv, Bulgaria. \nCurrently he is Associated Prof.Dr. at the Dental School, Oral Surgery Dept., Istanbul Aydin University and since 2003 he works in his own private clinic in Istanbul, Turkey.\u2028\nDr.Tosun is reviewer in journal ‘Laser in Medical Sciences’, reviewer in journal ‘Folia Medica\\', a Fellow of the International Team for Implantology, Clinical Lecturer of DGZI German Association of Oral Implantology, Expert Lecturer of Laser&Health Academy, Country Representative of World Federation for Laser Dentistry, member of European Federation of Periodontology, member of Academy of Laser Dentistry. Dr.Tosun presents papers in international and national congresses and has scientific publications in international and national journals. He speaks english, spanish, italian and french.",institutionString:null,institution:{name:"Istanbul Aydın University",country:{name:"Turkey"}}},{id:"171887",title:"Prof.",name:"Zühre",middleName:null,surname:"Akarslan",slug:"zuhre-akarslan",fullName:"Zühre Akarslan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/171887/images/system/171887.jpg",biography:"Zühre Akarslan was born in 1977 in Cyprus. She graduated from Gazi University Faculty of Dentistry, Ankara, Turkey in 2000. \r\nLater she received her Ph.D. degree from the Oral Diagnosis and Radiology Department; which was recently renamed as Oral and Dentomaxillofacial Radiology, from the same university. \r\nShe is working as a full-time Associate Professor and is a lecturer and an academic researcher. \r\nHer expertise areas are dental caries, cancer, dental fear and anxiety, gag reflex in dentistry, oral medicine, and dentomaxillofacial radiology.",institutionString:"Gazi University",institution:{name:"Gazi University",country:{name:"Turkey"}}},{id:"256417",title:"Associate Prof.",name:"Sanaz",middleName:null,surname:"Sadry",slug:"sanaz-sadry",fullName:"Sanaz Sadry",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/256417/images/8106_n.jpg",biography:null,institutionString:null,institution:null},{id:"272237",title:"Dr.",name:"Pinar",middleName:"Kiymet",surname:"Karataban",slug:"pinar-karataban",fullName:"Pinar Karataban",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/272237/images/8911_n.png",biography:"Assist.Prof.Dr.Pınar Kıymet Karataban, DDS PhD \n\nDr.Pınar Kıymet Karataban was born in Istanbul in 1975. After her graduation from Marmara University Faculty of Dentistry in 1998 she started her PhD in Paediatric Dentistry focused on children with special needs; mainly children with Cerebral Palsy. She finished her pHD thesis entitled \\'Investigation of occlusion via cast analysis and evaluation of dental caries prevalance, periodontal status and muscle dysfunctions in children with cerebral palsy” in 2008. She got her Assist. Proffessor degree in Istanbul Aydın University Paediatric Dentistry Department in 2015-2018. ın 2019 she started her new career in Bahcesehir University, Istanbul as Head of Department of Pediatric Dentistry. In 2020 she was accepted to BAU International University, Batumi as Professor of Pediatric Dentistry. She’s a lecturer in the same university meanwhile working part-time in private practice in Ege Dental Studio (https://www.egedisklinigi.com/) a multidisciplinary dental clinic in Istanbul. Her main interests are paleodontology, ancient and contemporary dentistry, oral microbiology, cerebral palsy and special care dentistry. She has national and international publications, scientific reports and is a member of IAPO (International Association for Paleodontology), IADH (International Association of Disability and Oral Health) and EAPD (European Association of Pediatric Dentistry).",institutionString:null,institution:null},{id:"202198",title:"Dr.",name:"Buket",middleName:null,surname:"Aybar",slug:"buket-aybar",fullName:"Buket Aybar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202198/images/6955_n.jpg",biography:"Buket Aybar, DDS, PhD, was born in 1971. She graduated from Istanbul University, Faculty of Dentistry, in 1992 and completed her PhD degree on Oral and Maxillofacial Surgery in Istanbul University in 1997.\nDr. Aybar is currently a full-time professor in Istanbul University, Faculty of Dentistry Department of Oral and Maxillofacial Surgery. She has teaching responsibilities in graduate and postgraduate programs. Her clinical practice includes mainly dentoalveolar surgery.\nHer topics of interest are biomaterials science and cell culture studies. She has many articles in international and national scientific journals and chapters in books; she also has participated in several scientific projects supported by Istanbul University Research fund.",institutionString:null,institution:null},{id:"260116",title:"Dr.",name:"Mehmet",middleName:null,surname:"Yaltirik",slug:"mehmet-yaltirik",fullName:"Mehmet Yaltirik",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/260116/images/7413_n.jpg",biography:"Birth Date 25.09.1965\r\nBirth Place Adana- Turkey\r\nSex Male\r\nMarrial Status Bachelor\r\nDriving License Acquired\r\nMother Tongue Turkish\r\n\r\nAddress:\r\nWork:University of Istanbul,Faculty of Dentistry, Department of Oral Surgery and Oral Medicine 34093 Capa,Istanbul- TURKIYE",institutionString:null,institution:null},{id:"172009",title:"Dr.",name:"Fatma Deniz",middleName:null,surname:"Uzuner",slug:"fatma-deniz-uzuner",fullName:"Fatma Deniz Uzuner",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/172009/images/7122_n.jpg",biography:"Dr. Deniz Uzuner was born in 1969 in Kocaeli-TURKEY. After graduating from TED Ankara College in 1986, she attended the Hacettepe University, Faculty of Dentistry in Ankara. \nIn 1993 she attended the Gazi University, Faculty of Dentistry, Department of Orthodontics for her PhD education. After finishing the PhD education, she worked as orthodontist in Ankara Dental Hospital under the Turkish Government, Ministry of Health and in a special Orthodontic Clinic till 2011. Between 2011 and 2016, Dr. Deniz Uzuner worked as a specialist in the Department of Orthodontics, Faculty of Dentistry, Gazi University in Ankara/Turkey. In 2016, she was appointed associate professor. Dr. Deniz Uzuner has authored 23 Journal Papers, 3 Book Chapters and has had 39 oral/poster presentations. She is a member of the Turkish Orthodontic Society. Her knowledge of English is at an advanced level.",institutionString:null,institution:null},{id:"332914",title:"Dr.",name:"Muhammad Saad",middleName:null,surname:"Shaikh",slug:"muhammad-saad-shaikh",fullName:"Muhammad Saad Shaikh",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Jinnah Sindh Medical University",country:{name:"Pakistan"}}},{id:"315775",title:"Dr.",name:"Feng",middleName:null,surname:"Luo",slug:"feng-luo",fullName:"Feng Luo",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Sichuan University",country:{name:"China"}}},{id:"423519",title:"Dr.",name:"Sizakele",middleName:null,surname:"Ngwenya",slug:"sizakele-ngwenya",fullName:"Sizakele Ngwenya",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"University of the Witwatersrand",country:{name:"South Africa"}}},{id:"419270",title:"Dr.",name:"Ann",middleName:null,surname:"Chianchitlert",slug:"ann-chianchitlert",fullName:"Ann Chianchitlert",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419271",title:"Dr.",name:"Diane",middleName:null,surname:"Selvido",slug:"diane-selvido",fullName:"Diane Selvido",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"419272",title:"Dr.",name:"Irin",middleName:null,surname:"Sirisoontorn",slug:"irin-sirisoontorn",fullName:"Irin Sirisoontorn",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"Walailak University",country:{name:"Thailand"}}},{id:"355660",title:"Dr.",name:"Anitha",middleName:null,surname:"Mani",slug:"anitha-mani",fullName:"Anitha Mani",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"355612",title:"Dr.",name:"Janani",middleName:null,surname:"Karthikeyan",slug:"janani-karthikeyan",fullName:"Janani Karthikeyan",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}},{id:"334400",title:"Dr.",name:"Suvetha",middleName:null,surname:"Siva",slug:"suvetha-siva",fullName:"Suvetha Siva",position:null,profilePictureURL:"//cdnintech.com/web/frontend/www/assets/author.svg",biography:null,institutionString:null,institution:{name:"SRM Dental College",country:{name:"India"}}}]}},subseries:{item:{id:"94",type:"subseries",title:"Climate Change and Environmental Sustainability",keywords:"Environmental protection, Socio-economic development, Resource exploitation, Environmental degradation, Climate change, Degraded ecosystems, Biodiversity loss",scope:"\r\n\tSustainable development focuses on linking economic development with environmental protection and social development to ensure future prosperity for people and the planet. To tackle global challenges of development and environment, the United Nations General Assembly in 2015 adopted the 17 Sustainable Development Goals. SDGs emphasize that environmental sustainability should be strongly linked to socio-economic development, which should be decoupled from escalating resource use and environmental degradation for the purpose of reducing environmental stress, enhancing human welfare, and improving regional equity. Moreover, sustainable development seeks a balance between human development and decrease in ecological/environmental marginal benefits. Under the increasing stress of climate change, many environmental problems have emerged causing severe impacts at both global and local scales, driving ecosystem service reduction and biodiversity loss. Humanity’s relationship with resource exploitation and environment protection is a major global concern, as new threats to human and environmental security emerge in the Anthropocene. Currently, the world is facing significant challenges in environmental sustainability to protect global environments and to restore degraded ecosystems, while maintaining human development with regional equality. Thus, environmental sustainability with healthy natural ecosystems is critical to maintaining human prosperity in our warming planet.
",coverUrl:"https://cdn.intechopen.com/series_topics/covers/94.jpg",hasOnlineFirst:!0,hasPublishedBooks:!1,annualVolume:11978,editor:{id:"61855",title:"Dr.",name:"Yixin",middleName:null,surname:"Zhang",slug:"yixin-zhang",fullName:"Yixin Zhang",profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002aYWJgQAO/Profile_Picture_2022-06-09T11:36:35.jpg",biography:"Professor Yixin Zhang is an aquatic ecologist with over 30 years of research and teaching experience in three continents (Asia, Europe, and North America) in Stream Ecology, Riparian Ecology, Urban Ecology, and Ecosystem Restoration and Aquatic Conservation, Human-Nature Interactions and Sustainability, Urbanization Impact on Aquatic Ecosystems. He got his Ph.D. in Animal Ecology at Umeå University in Sweden in 1998. He conducted postdoc research in stream ecology at the University of California at Santa Barbara in the USA. After that, he was a postdoc research fellow at the University of British Columbia in Canada to do research on large-scale stream experimental manipulation and watershed ecological survey in temperate rainforests of BC. He was a faculty member at the University of Hong Kong to run ecological research projects on aquatic insects, fishes, and newts in Tropical Asian streams. He also conducted research in streams, rivers, and caves in Texas, USA, to study the ecology of macroinvertebrates, big-claw river shrimp, fish, turtles, and bats. 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