Open access peer-reviewed chapter

Chitosan and Xyloglucan-Based Hydrogels: An Overview of Synthetic and Functional Utility

Written By

Diana M. Martínez-Ibarra, Jaime López-Cervantes, Dalia I. Sánchez- Machado and Ana Sanches-Silva

Submitted: October 4th, 2017 Reviewed: January 30th, 2018 Published: July 18th, 2018

DOI: 10.5772/intechopen.74646

Chapter metrics overview

1,699 Chapter Downloads

View Full Metrics


The development of new strategies for wound healing has resulted in the design of biomedical devices using polymers of natural origin. Hydrogels are biomaterials formed by three-dimensional polymeric networks that can retain large amounts of water or biological fluids, and smooth texture similar to living tissue. Chitosan is a linear polysaccharide, (1-4)-2-amino-2deoxy-ß-D-glucan, which has desirable features such as biocompatibility, non-toxicity, hemostasis and antibacterial character. Xyloglucans have different applications in tissue engineering for their physicochemical properties, biocompatibility and control of cell expansion. Hydrogels had been made of homogeneous mixtures prepared of chitosan and purified xyloglucan, followed by a freeze-drying process to develop a flexible and porous structure. Additionally, their mechanical properties such as porosity, solubility, biodegradation, and the antibacterial activity of the hydrogels are studied. The results suggest that the incorporation of xyloglucan favors the characteristics from chitosan-based hydrogels, providing a promising alternative for application in biomaterials with antimicrobial activity.


  • chitosan
  • chitin
  • xyloglucan
  • hemicellulose
  • hydrogels
  • biocomposites
  • biomaterials
  • polysaccharide

1. Chitosan

1.1. Chemical properties and production

Chitosan is an amino-polysaccharide of natural origin. This polymer consists of a linear chain of repeating monomers of D-glucosamine and N-acetyl-D-glucosamine, whose contents and sequence are variable [1]. The amino groups allow specific chemical reactions and confer very important functional properties [2]. It is usually, produced by the partial deacetylation of chitin, a linear polymer of N-acetyl-2-amino-2-deoxy-D-glucopyranose linked with β-(1-4) bonds [3].

It is estimated that approximately 10 million tons of chitin, can be synthesized in nature every year [4]. Chitin and its derivatives are renewable, biocompatible, non-toxic, biodegradable and have biological properties such as anti-cancer, antioxidant, antimicrobial and anticoagulant [5]. It is mainly found in the exoskeleton of crustaceans such as shrimp and crab with contents from 58 to 85%, which are the most important source of chitin for commercial use due to their availability as waste produced during its industrial processing. These residues in turn constitute to one of the main problems of these industries for society because of its negative impact on the environment [5, 6]. Also, chitin is a structural component of the cell wall of fungi such as Aspergillus niger and Mucor rouxii with up to 45% [7]. Some marine invertebrates contain from 3 to 28% of chitin. In squid pens, 31–49% of chitin has been reported [8]. Moreover, in a recent investigation it has been reported that insect larvae and imagos are an alternative source of isomorphic α-chitin, in a range of 20–60% [4, 9], authors reported 14% of α-chitin [10] in grasshoppers (Dociostaurus maroccanus).

Chitin exists in three major polymorphic forms, α, β and γ-chitin. These differ in the arrangement of the chains within the crystalline regions [11]. α-chitin is the most stable and abundant form [5]. It possess a compact rhombic structure, due to the antiparallel chain that favors the formation of interlaminar hydrogen bonds [12] between the hydroxyl and carbonyl groups [13]. Conversely, the β-chitin structure is monoclinic with a parallel arrangement that inhibits the formation of interlaminar hydrogen bonds. In some studies, it have been reported that β-chitin has a higher solubility, reactivity and affinity to polar solvents than α-chitin [14, 15]. γ-chitin, is a combination of the α- and β-chitin configurations, has been found in the stomach of squid and in the buds of beetles. Squid pens, extracellular fibers of diatoms and spines of annelids are sources of β-chitin [5], while α-chitin is isolated from the exoskeletons of crustaceans, particularly shrimp and crab [15].

Traditionally, the extraction of chitin from exoskeletons of crustaceans consists of a treatment with hydrochloric acid in order to remove inorganic components such as calcium carbonate and calcium phosphate. This is followed by an alkaline treatment with NaOH to solubilize the proteins and remove some pigments such as melanin and carotenoids [16] temperature control and concentration of NaOH are crucial to achieve a satisfactory result. In addition to the chemical methods for obtaining chitin, biological methods involving the use of microorganisms [17] and enzymatic hydrolysis [18] have been reported.

One of the limitations in the use of chitin on a large scale is its insolubility in water, due to which water-soluble derivatives are produced, and chitosan being the most important of them [5]. Once the decalcification and deproteinization steps are completed, chitosan is obtained by alkaline deacetylation of chitin using a saturated solution of NaOH 45% [19, 20]. The deacetylated form of chitosan in acidic solutions offers the advantage to be efficiently processed as powder, pastes, gel, membranes, sponges, beads, microparticles, nanoparticles and nanofibers [21]. Some methodologies for the production of chitosan by chitin deacetylation are presented in Table 1.

ReferenceSources of chitinConditions/chemical methodProperties
[11]Crab shellsNaOH 12 M
Under nitrogen atmosphere 110°C (2–3 h)
[25]Shrimp shellsNaOH 50%, ratio 1/50 g mL−1
Under nitrogen atmosphere, 100°C (9 h)
DD = 80%
[26]ShellfishNaOH 47%, ratio 1/10 g mL−1
Under nitrogen atmosphere 110°C (30 min)
DA = 26.9 ± 0.8%
[13]Prawn shellsNaOH 40%
High intensity ultrasound irradiation
DA = < 32%
1 × 105 g mol−1 < Mv < 2 x 10 5 g mol−1
[27]Shrimp shellsNaOH 50%, ratio 1/20 g mL−1
Intensity 350 W (8 min)
DD = 82.73%,
Mw = 2.3 × 10–18 ± 137 g mol−1
[28]Squid pensNaOH 40%, ratio 1/10 g mL−1
Ultrasound irradiation, 60°C (50 min)
DA = 36.7%, Mv = 10.3 ± 0.3 x 105 g mol−1, Mw = 12.6 ± 0.4 x 105 g mol−1
[29]Lobster by-productsNaOH 50%, ratio 1/10 g mL−1
120°C (4 h)
DD = 71.59%
Enzyme/biological method
[30]Deacetylase chitin from Colletotrichum lindemuthianu Reaction performed at 45°C
[31]Shrimp shellsDeacetylase chitin from Mucor rouxii
Reaction performed at 50°C
FA = 0.582, 0.400 y 0.188
[32]Shrimp shellsDeacetylase chitin from Pichia pastoris
Reaction performed at 50°C (60 min)
DA = 33%
[33]Crab and shrimp shellsChitinase isolated from the stomach of Parapristipoma trilineatum
Reaction performed at 37°C (2 h)
[34]Deacetylase chitin from Absidia orchidis vela coerulea
Reaction performed at 50°C (250 h)
[35]Steam explosion (SE) High pressure (1 Mpa), at 180°CDA = 3.7%
Reduction of crystallinity index 11.28%
[12]Shrimp shells and squid pensSteam explosion (SE) (9 Kg/cm2), at 179°CDD = 42.9% (α-chitin)
DD = 43.7% (β- chitin)

Table 1.

Chemical and biological methods for the chitin deacetylation.

DA, degree of acetylation; DD, deacetylation degree; Mw, weight average molecular weight; Mv, viscosity average molecular weight; FA, fraction of acetylated units.

Chemical method for the preparation of chitosan provides a degree of deacetylation of 85–93%, products with a wide range of molecular weight are obtained [22]. Studies have shown that the enzymatic conversion offers a degree of deacetylation up to 97%, and could generate new polymers with different characteristics. Steam explosion is a hydrothermal method to deacetylate chitin, where the biomass is treated with saturated steam at high pressure and temperature for minutes, followed by an explosive decompression; during the process, molecular interactions are broken by thermo-mechanical forces [12]. It has been proposed to differ between chitin and chitosan based on their solubility in acid solutions, that is, if chitosan is soluble and chitin is insoluble [16].

Chitosan oligomers are short fragments of chitosan composed by the same units and glycosidic bonds, commonly oligomers are obtained by chemical or enzymatic methods [23]. They are named according to the number of sugar rings in their chemical structure (dimer, tetramer and hexamer). Compared with conventional chitosan and its derivatives, chitosan oligomers have relatively lower molecular weight and attributed remarkable characteristics of water solubility [24].

The confection of chitosan with respect to the degree of polymerization, polydispersity, degree of acetylation, molecular weight and acetyl group distribution provides tools to manipulate functions and properties in regard to their biological effects and/or applications [18, 23]. These parameters are important to examine the relation of structural units between N-acetyl glucosamine and glucosamine, for example, in the case of N-acetylation degree, the molecular weight depends on the source of obtention and the deacetylation conditions [16] during the conversion process, that is, temperature, time of exposure and alkali concentration.

1.2. Biological properties

Chitosan is one of the most widely used natural biopolymers due to its high biocompatibility, biodegradability, non- toxicity, bioadhesivity, antigenic capacity and hemostasis [23, 36, 37, 38]. In the materials science, biocompatibility is defined as the absence of cytotoxicity of a biomaterial and its biofunctionality that allows it to support cell-biomaterial interactions [39]. The evaluation of the biocompatibility of the implantable systems requires an understanding of the inflammatory and curative responses of each material. Inflammation, scarring and response to foreign bodies are tissue responses to injury [40]. Bioadhesivity refers to the ability of the polymer to adhere to hard or soft tissues [41]. It adheres to epithelial tissues and the mucous coating present on the surface of tissues [37]. Clinically, when the chitosan biocomposites come in contact with a wound, it adheres to covering the site of the lesion and attracts the red blood cells, forming a seal that prevents further bleeding [42]. Chitosan hemostatic mechanism involves agglutination of blood cells, possibly due to its intrinsic polycationic properties and non-specific binding to cell membrane [43]. Research has led the addition of new formulations to adapt the biocomposites to the needs of the injury. Researchers studied the gelatin-chitosan interaction for sponge formulation as a hemostatic agent [4, 44]. Chitosan-based hydrogel sheets with honey and gelatin have been made as a coating for burn wounds [45]. Authors reported the development of chitosan-agarose hydrogels for tissue engineering application [46]. Chitosan biocomposites treated with sodium hydroxide (NaOH) and sodium tripolyphosphate (Na5P3O10) for hemostatic use have been developed [42]. Phosphate incorporation as a precoagulant and silver nanoparticles as antimicrobial agent into biocomposite-based chitosan has resulted in the blood clotting acceleration, platelet adhesion and significantly absorb more blood than chitosan biocomposites [47].

Researchers defined biodegradation as an event that takes place through the action of enzymes and/or chemical decomposition associated with living organisms and their secretion products [48]. The final result is a loss of structural integrity and radical decrease of molecular weight [4, 49].

The biodegradable chitosan effect is attributed to lysozyme, an existing enzyme in several plants and in the human body [4, 21, 50], which is produced by macrophages during wound healing [51]. It is known as a glycoside hydrolase that possess the ability to slowly hydrolyze the β-(1-4) N-acetylmuramic acid and N-acetyl-D-glucosamine bonds or between N-acetyl-D-glucosamine residues [52, 53] of chitosan membranes. Moreover, promotes tissue granulation, increases the expression of collagen among other components of the extracellular matrix and accelerates wound healing [54, 55]. Additionally, it acts by enhancing the proliferation and migration functions of inflammatory cells such as polymorphonuclear leukocytes (PMN), macrophages and fibroblasts [56] at the site of injury [6, 57]. The N-acetylglucosamine of chitin and chitosan are the major components of dermal tissue, and is essential for the wound repair; in addition, its positive surface charge allows it to be a support for cellular development and promotes blood clotting [58, 59].

1.3. Antimicrobial activity

An ideal antimicrobial polymer must be economically and simply synthesized, stable in long term, soluble in water or neutral medium, should not be decomposed or emit toxic products, must possess bactericidal activity against to a broad spectrum of pathogenic microorganisms in brief times of contact [16]. Chitosan has been shown to have advantages over other disinfectants, due its high antimicrobial capacity, a broad spectrum of activity and higher mortality rate [14, 60]. In medicine, wounds caused by burns are highly susceptible to infection by skin deterioration that acts as a barrier against microorganisms [54]. Researchers report that biocomposites with more than 0.025% of chitosan inhibits the growth of Escherichia coli, Fusarium, Alternaria and Helminthosporium [21]. Studies about the antimicrobial activity of chitosan, honey and gelatin hydrogels as possible coatings for burn injuries reported antibacterial efficiency against S. aureus and E. coli [45]. Chitosan-gelatin composites have presented similar inhibitory activity against Gram-positive and Gram-negative microorganisms [61]. PVA addition to chitosan solutions for nanofibers productions with multiple applications reported bacteriostatic activity against E. coli [62]. According to investigations, the use of chitosan sponges for diabetic foot ulcers treatment prevents polymicrobial infection and decrease the risk of amputation [63].

Chitosan it is a potent antimicrobial agent of cationic nature at pH below 6.3 [62]. An antimicrobial agent is one that eliminates microorganisms or inhibits its growth [21]. Some hypotheses indicate that chitosan could interact with anionic groups on the cell surface of microorganisms increasing the permeability of the membranes, facilitating the scape of proteins and other intracellular constituents of the microorganisms [61]. Another mechanism involves the formation of chitosan, chelates with trace elements or nutrients, resulting in the enzymatic activity inhibition [64] due to the chitosan-DNA interaction that modifies the synthesis of RNA messenger [7]. The antimicrobial effects are regulated by intrinsic factors including the type of chitosan, degree of polymerization, the source, chemical composition of substrates (e.g., moisture and/or water activity) and environmental conditions [21]. Research on antimicrobial properties of chitosan films with different deacetylation degree (DD) and molecular weight, against Gram-positive and Gram-negative bacteria demonstrated that the inactivation step of the bacteria increases with the increase in deacetylation degree of the biopolymer; however, the bacteriostatic and bactericidal mechanism of action is not fully known [65].

1.4. Structural properties

At neutral or basic pH, chitosan contains free amino groups and is insoluble in water, while in acidic pH it is soluble in water, due to the protonation of its amino groups [21]. Chitosan exhibits unique polycationic characteristics, chelating properties and film forming abilities, due to the presence of amino and hydroxyl active groups [64]. It is widely used to prepare natural hydrogels, however, they generally lack mechanical stability unless they are cross-linked and/or reinforced by suitable compounds [66]. By definition, hydrogels are polymer networks having hydrophilic properties [67, 68]. They have been used in the pharmaceutical and biomedical area for wound care, as drug releasers, organ and tissue transplantation [69]. The incorporation of therapeutic agents into the hydrogel formulations has been used in order to facilitate many healing processes, particularly in burn wounds [70].

Hydrogels are defined as three-dimensional, hydrophilic networks capable of swelling and absorbing large amounts of water or biological fluids [71], when deposited in aqueous solutions. Hydrogels containing more than 95% water are called as “superabsorbents” and have a high biocompatibility due to their high degree of water retention [72], which is due to its high water content, porosity and soft consistency that are very similar to the natural living tissues [73].

Physical hydrogels are the result of environmental changes (temperature, pH, molecular arrangements and supramolecular interactions), which have the advantage of forming gels under mild conditions, without the use of organic solvents, while chemical hydrogels can be produced by radical polymerization, chemical reactions and/or enzymatic reticulation that also possess better mechanical properties; however, they need solvents and/or toxic crosslinkers [74].

The cross-linking of chitosan can be achieved with the implementation of chemicals products such as epichlorohydrin or glutaraldehyde to enhance their stability in acid solutions [75]. Researchers studied the combination of two cross-linking methods, ionic with CaSO4 and covalent with genipin for the preparation of chitosan-based hydrogel films [76]. They found that ionic and covalent cross-linking exhibit differences in mechanical characterization (strength and maximum load). Investigators designed thermosensitive hydrogel-based chitosan and its derivatives, without the addition of chemical cross-linking agents [77], showed that it may be a viable alternative as a vehicle for the release of injectable drugs. Further studies on the behavior of chitosan-agarose hydrogels as a biocomposite for tissue regeneration [78], determined that the hydrogel provides an adequate environment for healing, that is, meets the criteria for an ideal wound dressing. Chitosan cross-linking with poly(alginic acid) for the manufacture of nanohydrogels (30–80 nm) with ability to remove aqueous metals, reported excellent absorption abilities for Cr (IV) removal [79].

The structures formed in the chitosan hydrogels are: (a) chitosan cross-linked with itself, that is, without the need for any additives, the process that is based on the neutralization of the amino groups of chitosan and thus the inhibition of repulsion between the chains of the polymer [73, 80]. (b) Hybrid polymer network consists of the mixing of two polymer solutions, which commonly use the same solvent, with or without the addition of cross-linking agents [81]. (c) Semi-interpenetrating polymer network is formed when a linear, biological or synthetic polymer is trapped within a polymer matrix [71] and (d) ionic cross-linking are considered as biocompatible have a non-permanent network formed by reversible bonds and have a greater sensitivity to swelling in pH changes [50]. Depending on the nature of the cross-linker, the main interactions that form the network are ionic or covalent bonds [21].

1.5. Other uses

In addition to the biomedical and pharmaceutical area, the multiple properties of chitosan have made it a polymer of interest for food preservation, agriculture and water treatment, among others (Figure 1).

Figure 1.

Potential applications of chitin and chitosan.

It has been used in water treatment as a flocculating polymer [66]. Chitosan has high efficiency in the removal of organic pollutants, suspended solids and metal ions in comparison with commercial chemical flocculants [82, 83]. In addition, its potential use as a natural coagulant in the hybrid membrane coagulation-nanofiltration process for water treatment has been studied [84], also the manufacture of hollow fiber nanocomposites in the removal of chemical compounds from water [85]. Chitosan even has been applied as a simple films for the selective removal of mercury in multimetal solutions [86].

During food production, the packaging is an important part to ensure its integrity, in this sense, the food industry has put special interest in the application of materials with antimicrobial capacity. Edible coatings can be applied in liquid form while edible films are made as solid sheets and can be used to wrap food products, its application improves the quality and extends the shelf life of slightly elaborated products [87, 88, 89].

Panczyk et al. [65] evaluated the antimicrobial properties of chitosan-gelatin films, and found that Pseudomonas fluorescens and Listeria innocua were more sensitive to chitosan than Escherichia coli and Staphylococcus aureus. Studies have reported effective antimicrobial properties against Listeria monocytogenes in chitosan films added with plasticizers [19] (Sorbitol and glycerol) for active packaging use. Similar results were reported by Coma et al. [90] in chitosan solutions for the production of edible films. Leceta et al. [91] reported a bacteriostatic behavior of high and low molecular weight chitosan films against E. coli and L. plantarum, which cause the decomposition of food. Bourtoom et al. [92] investigated the mixture of chitosan and rice starch for the elaboration of edible films as an alternative to commercial packaging materials. Its applications have been studied as a cholesterol-lowering agent and its application as an agent for weight reduction [16].

Chitosan has been shown to stimulate plant growth and promote tolerance to abiotic and biotic stress in various horticultural products [93]. It has been studied that the application of chitosan, controlled the release of agrochemicals and genetic materials, and they function as a reservoir of protection for active ingredients [94].

In the cosmetic industry, it has been incorporated in the elaboration of shampoos, conditioners and hair coloring agents. Additionally, in deodorants, and for moisturizing the skin, it could compete with hyaluronic acid [18]. It can also be used for the formulation sunscreens, minimize acne problems, and reduce static electricity of hair, among others [37].

The textile industry has made use of biodegradable polymers for the manufacture of towels, filters and geotextiles for the control of erosion and landscaping [48]. The lack of commercial chitosan-based products could be attributed to the several challenges when working with it [95].


2. Xyloglucan

2.1. Chemical structure and sources

Hemicelluloses are branched polymers, and the main monomers found are D-glucose, D-mannose, D-xylose, D-glucuronic acid, 4-O-methyl-D-glucuronic acid and D-galacturonic acid [96]. Together with lignin, they form the microfibrils surrounding cellulose [95].

Xyloglucans (XG) are structural polysaccharides and the major components of hemicellulose [97]. They are localized in the middle lamellar and gelatinous layer of the primary cell walls of the superior plants [98, 99]. XG are structurally related to cellulose, as it is associated in a non-covalently form within the cell walls of plants [100].

Xyloglucans or generally called galactoxyloglucans, possess a main chain identical to cellulose, is a glucose polymer linked by β-(1-4) bonds, with side residues of xylose linked by bonds 1–6 along the backbone. The major structural differences of the polysaccharide occur when galactosyl and fucosyl-galactosyl residues are added to the xylose residues in dicotyledons, as well as less residues of xylosyl in monocotyledons [96, 99].

Xyloglucans can be extracted from different species as Copaifera langsdorffii, Hymenaea courbaril and Tamarindus indica. Some investigations indicate that they are composed by the same monomers, nonetheless, their proportion and distribution result in a fine structure, which varies according to the species and even within the same species [101]. The XG commercialized on a large scale is extracted from the tamarind seeds [102]. Table 2 describes the morphological characteristics of plants that produce seeds as a source of xyloglucan as well as their potential applications.

SourcePlant description/cultivation areaXyloglucan contentApplications
Chia seed (Salvia hispanica L.)Seeds are 2 mm long, with a diameter 1.5 mm/from the region center-west of Mexico to north of Guatemala4–6% [105]Edible films [106]; pharmacology industry and nanocomposites [107]; drug delivery excipients for site-specific release and transdermal drug delivery agents [105]
Seed kernel of tamarind (Tamarindus indica L.)Adult tree (20–30 m, with a trunk diameter (1.5–2 m), Seeds are 1.6 cm long/India, Africa, Pakistan, Bangladesh, Nigeria and most of the tropical countries.Abundant deposits of xyloglucan [108]Drug delivery [98]; stabilizer, binder and gelling agent [109]; food thickener, sizing agent in textile, paper and jute industries [110]
Seeds of Copaifera langsdorfiiAdult tree (up to 35 m), seeds are 1.5 cm long/forest and savanna populations40% [111]Potential use in the pharmaceutical, food or cosmetic industries [112]
Seed of Hymenaea courbaril L. (Jatobá)Adult tree produces an average of 10 kg of seed. Seeds are 1.5 cm long with a diameter of 2.5 cm/Neotropical region of the world40–50% [113, 114]Useful as partial substitute for agar in culture media for micropropagation of apples [115]
Seed of Guibourtia hymenaefoliaAdult tree (10–18 m, with diameters 40–70 cm) produce approximately 1400 seeds per kg/Congo, Equatorial Africa, Nigeria, Cameroon Gabon and other tropical regions54.20%Food technologies and biotechnological processes, pharmaceutical and medical industries [116]
Seed of Detarium senegalenseSmall tree (5–7 m high)/mainly found in West Africa, Chand and SudanAbundant deposits of xyloglucan [108]Pharmaceutical (for controlling drug release) and food industries [117]

Table 2.

Description of plants as xyloglucan sources and its applications.

An alternative source of xyloglucan is chia seed (Salvia hispanica L.). This ancient seed is native from the central-west Mexico region to the north of Guatemala, where it was consumed by the Aztecs; however, at present it has spread to other regions. The seed has essential fatty acids such as linoleic acid (ω-6) and α-linoleic acid (ω-3), additionally, it is a source of phenolic compounds that provide various effects as antioxidant, antitumor, antithrombotic and anti-inflammatory [103]. It possess high fiber content formed of natural sugars [104].

When the seed is placed in contact with water, the gum or mucilage is secreted by chia seed (Figure 2), covering it with a transparent halo [118]. This mucilage represents 5–6% of the total weight of the seed, and is described as an anionic heteropolysaccharide with a high molecular weight (800–2000 kDa) [106]. Structurally xyloglucan is constituted by xylose monomers linked to glucose monomers in a 2:1 ratio [105]. A tentative polysaccharide structure is a tetrasaccharide with the main chain composed by (1-4)-β-D-xylopyranosyl-(1-4)-α-D-glucopyranosyl-(1-4)-β-D-xylopyranosyl units with 4-O-methyl-α-D-glucuronic acid ramifications in the O-2 position of β-D-xylopyranosyl main chain [119].

Figure 2.

Extraction and potential uses of xyloglucan from chia (Salvia hispanica L.).

Hymenaea courbaril L. (legume tree) seeds store xyloglucan, which has a major chain of β- (1-4) glucose units, with some ramifications α-(1-6) xylanopiranosyl or β-(1-2)-D-galactopyranosyl- α-(1-6)-D-xylaropiranosyl [113].

Aspen wood, is a source of xylan composed by a linear chain β-(1-4), linked to a D-xylose with a 4-O-methyl-α-D-glucuronic acid replacing the 2-position of approximately every 8 xylose units [120]. Various sources of hemicellulose and the monomers that compose them are presented in Table 3. Aspen wood hemicellulose fractions contain mainly xylose monomers and in a less proportion arabinose and glucose , in comparation with abedul wood commercial xylan [121].

[98, 122]Seed kernel of Tamarindus indicaXylose-glucose (3:1)
[105]Chia seed (Salvia hispanica L.)Xylosa-glucose (2:1)
[118, 119]Chia seed (Salvia hispanica L.)Xylose, glucose and glucuronic acids (2:1:1)
[120]Aspen wood (Populus tremula)Xylose: glucuronic acids
[66]Pine woodArabinose, galactose, glucose, mannose and xylose
Switchgrass (Panicum virgatum)Arabinose, galactose, glucose, mannose and xylose
Coastal bermudagrassArabinose, galactose, glucose and xylose
[101]Hymenaea courbaril seedGlucose: xylose: galactose (4:3:2)
[100]Quinoa (Chenopodium quinoa W.)Glucose: galacturonic acid: arabinose: galactose: mannose: xylose
Amaranth (Amaranthus caudatus L.)Glucose: galacturonic acid: arabinose: galactose: mannose: xylose
[123, 124]Almond GumArabinose: galactotose: xylose: glucose: rhamnose: glucuronic acid
[125]Neolamarckia cadamba (Rubiaceae)Xylose: galactose: rhamnose: glucuronic acid: mannose: fucose

Table 3.

Monomers and hemicellulose extraction sources.

The monosaccharides composition from three different biomasses studied by liquid chromatography, reported a composition of arabinose: galactose: glucose: mannose: xylose (2.2: 1.4: 1.3: 4.7:13.5) for pine wood, arabinose: galactose: glucose: mannose: xylose (3.1: 0.8: 1.0: 0.2: 21.8) for switchgrass (Panicum virgatum) and for coastal bermudagrass, the composition was arabinose: galactose: glucose: xylose with a 4.4: 1.9: 0.8: 22.0 proportion, respectively [66].

Glucose, galacturonic acid and arabinose are the principal monomers found in quinoa (Chenopodium quinoa W.) and amaranth (Amaranthus caudatus L.) identified by gas chromatography [100]. In almond gum, the main hemicellulose monomers identify by gas chromatography are galactose, arabinose, xylose, mannose, rhamnose and glucuronic acid with 45:26:7:10:1:11 molar ratio, respectively [123].

The composition of hemicellulose monomers studied by acid hydrolysis and HPLC analysis in three fractions (apical, middle and basal) of Neolamarckia Cadamba [125], reported that xylose is the main monomer on the three fractions. The mannose glucuronic acid, rhamnose, galactose and fucose content is in the range 22–28%.

2.2. Extraction methodologies

Recent research has shown interest in the xyloglucans extraction from several vegetal sources. The methodologies involve the hydration of matter in water, the methods major differences are based on the raw material/ water (w/v) proportion, temperature (°C), drying methods (vacuum drying or lyophilization) and the process of seeds separation (filtration or sieving).

Specifically, for the mucilage extraction from chia seed, the treatments involve hydration with distilled water (1:40, w/v) at 80°C, for 2 h. Finally, the gum drying process is carried out at 50°C and the seed detachment is performed by sieving [119]. Another method involves soak the seed into water (1:30 w/v, ratio) at 25°C for 2 h to moisturize the chia seed, afterwards the mucilage is separated by centrifugation and vacuum filtration to remove the solid waste, subsequently the gum is stored as lyophilized material [106].

Capitani et al. [118] discuss two methods for chia seed mucilage extraction, in the first, it is proposed to submerge the seeds in distilled water (1:10, w/v) for 4 h, followed by a lyophilization process, the mucilage separation occurs by friction in a sieve. The second method involves the same water repose and vacuum filtration separation, followed by a pre-concentration on a rotary evaporator and complemented by lyophilization. After evaluating its rheological properties, it was concluded that the second extraction method provides a greater consistency to the mucilage.

Simi et al. [97] studied the xyloglucan physicochemical properties extracted from tamarind seeds. The procedure involves a pulverized seed deproteinization step with protease (at 30 ± 2°C, pH 6). For grease extraction, they used hexane as solvent, subsequently a 95% (v/v) ethanol solution was used to precipitate the XG, once extracted it is lyophilized and sprayed before use.

Alternative methods of hemicellulose extraction involve alkaline treatments with NaOH combined with an ultrafiltration process. The previous has been applied to aspen wood (Populus tremula), the final product is obtained by spray drying [120].

Xyloglucan extraction from Hymenaea courbaril L. is carried out with 80% ethanol (80°C, for 10 min). Water is then added and maintained at 80°C for 3 h. The insoluble material is extracted with 4 M KOH. The polymer extracted with alkali is neutralized with acetic acid, followed by a dialysis process and lyophilization [113].

Researchers performed a hemicellulose alkaline extraction from sugarcane bagasse with NaOH (1:25, w/v), to precipitate the hemicellulose, four different ethanol solutions were tested and the pellet was dried at 40°C for 24 h [126] the extract was used to prepare biodegradable films.

Arruda et al. [101] studied the biological activities of xyloglucan extracted from courbaril seeds (Hymenaea courbaril). The extraction procedure included enzymes inactivation, as well as treatments with NaCl and ethanol at 46% (1:3, v/v) to precipitate the gum.

In another trial, alkaline extraction and delignification with toluene-ethanol (2:1, v/v) is proposed, for obtaining Neolamarckia cadamba (Rubiaceae) hemicellulose monomers. The ethanol precipitate was finally lyophilized [125].

2.3. Properties

Xyloglucans possess important applications, especially in pharmaceutical formulations for the gel production [122]. Furthermore, they participate in the control of cellular expansion, own an effect on cell growth, and act as a seeds carbon reserve of many dicotyledons. XG are neutral, non-mutagenic, non-irritating, non-toxic and blood compatible [127, 128]. Additionally, increase the viscosity, have wide pH tolerance, high temperature regimes resistance and salt, also possess adhesiveness, non-carcinogenicity and biocompatibility properties [101, 102]. Considering its attributes, xyloglucans have promising biotechnological purposes [129].

This polysaccharide is considered a hydrocolloid, as a result of its viscosity and ability to retain water, particularly when highly viscous solutions are formed. Currently they are used in a wide range of industries for different applications [118]. Other investigators have attributed properties such as thickener, binder, as a controlled release, texture modifiers, gelling agent, emulsion stabilizers and syneresis control [106, 119]. In Japan xyloglucan is widely used as a food additive, provide texture and can be used in combination or replacement of starch [108]. The previous attributes are due to the xyloglucan possess a high viscosity degree and stability in acid pH and resistance to high temperatures [130].

Researchers evaluated the solubility and water vapor transmission rate (WVTR) effect in xyloglucan films from sugarcane bagasse [126] concluded that the purification process intervenes directly in the micro-structural properties of biodegradable films. In agreement with other authors, xyloglucan films can be industrially used as coatings in ready-to-eat foods and with health benefits because of their high content of soluble fiber [106].

Biosorption is the property possessed by some biomolecules to bind and concentrate selected ions and other molecules in aqueous solutions [131]. Hemicelluloses mainly conformed by xyloses have application field in the ecology. Thus their combinations with biopolymers such as chitosan have been investigated to produce biosorbent materials in the desalination and heavy metals (Ni, Cu & Pb) removal from water [66]. Other research suggests its application for the development of flocculant-adsorbents for remove several types of dyes from textile wastewater [132].

Bioadhesion can be defined as the state in which two materials, being at least one of them from biological nature, are maintained together by interfacial forces for long periods of time [133]. Natural polymers have been widely used as bioadhesives because of their biocompatibility, specifically the xyloglucan extracted from tamarind seeds has been studied as a mucoadhesive polysaccharide for the transport of medicament administered through the oral route [109].


3. Chitosan-xyloglucan hydrogels

3.1. Preparation

The use of natural polymers with different mechanical, physical and biological properties is frequent in the design and development of biomedical matrices [134]. Biopolymers, which include polysaccharides such as cellulose, chitosan, wool, silk, gelatin and collagen, have been found promising for multitudinal applications in different forms [21].

Chitosan is compatible with a wide variety of biologically active components [18]. The inclusion of carbohydrates such as glucose, cellulose and hemicellulose in chitosan particles generate changes in their structure and by consequence in the biomaterials properties [135]. However, the addition of biopolymers such as xyloglucan (hemicellulose) for the formulation of hydrogels confers resistance properties that increase the value and suitability of the polymer. The main component of chitosan is glucosamine, being a natural substance produced by the body from glucose and it is related to the production of glycosaminoglycans (GAG) that form cartilage tissue in the body and that is also present in ligaments and tendons. It is a biocompatible material that slowly decomposes into harmless products that are completely absorbed in the body [21].

The antimicrobial effect of chitosan has been shown to be beneficial for its application as implants and drug liberators [23]. Hydrogels have attracted attention in various investigations because of their great ability to absorb liquids and their swelling-deswelling capacities sensitive to stimuli without disintegration, what makes it of interest in biomedical and pharmaceutical applications [36, 67]. The swelling is associated by itself with the bioadhesiveness, this depends on the concentration of the polymer, ionic strength, as well as the presence of water, during the dynamic process of bioadhesion, the maximum bioadhesion in vitro occurs with a optimal water content [41]. The addition of xyloglucan for the formulation of chitosan-xyloglucan hydrogels increases the swelling capacity, because it increases the amount of hydrogen bonds and facilitates the absorption of water [135]. Investigations on the formulation of films from chitosan-hemicellulose demonstrated the capacity and biocompatibility for the application of coatings for wounds, because both polymers are of natural origin and the native properties of chitosan and xyloglucan are beneficial for cell growth [2].

During the healing process, it is indispensable to count on wound coatings for regenerate and repair dermal and epidermal tissue. For a passive coating is essential to stimulate wound healing and to preserve a humidifying environment [70, 136]. In addition, it should prevent the loss of body fluid, prevents accumulation of exudate and protects the lesions from external contamination [21].

Hydrogels have attracted attention because their three-dimensional polymer networks [74] have high capacity to absorb and retain large amounts of water, saline or physiological solutions [137] and present stimulus of response to the swelling-deswelling without disintegration [121]. They owe their mechanical stability to the cross-links introduced between the macromolecular chains that allow flexibility and sufficient resistance [66]. From the science of materials point of view, biological tissues, the essentially moist and soft materials have an elastic modulus of 104–107 Pa and a water content of 50–85% [138].

In Table 4, some biocomposites formulated with mixtures of chitosan, xyloglucan, crosslinkers and other materials are described. Some hydrogels experience continuous and discontinuous changes in swelling, these are regulated by external stimuli such as changes in pH, temperature, ionic strength, solvent type, electric and magnetic field, light and the presence of chelating agents [129].

[139]Chitosan: celluloseFilms
[110]Plasticized xyloglucanFilms
[122]Bacterial cellulose/xyloglucan (10, 20, 30 wt%)Films
[106]Xyloglucan (1%) - glycerol as plasticizer (25, 50 and 75% w/w), based on XG weight.Films
[2]Chitosan (0.1%): Hemicellulose, (1:1)Films
[121]Chitosan 1%: hemicellulose 1% (70:30, 30:70)Hydrogels
[128]Oxidized xyloglucan-chitosan (1:0.5, 1:1, 1:2, 1:3, 1:4) in acetic acid solutionTransparent hydrogels
[140]Chitosan: xylan hemicellulose, (3:1)Hydrogels for bone tissue regeneration
[141]Pharmaceuticals coated with xyloglucan (0.5% or 3%)Nanocomposites for drug delivery
[96]Hemicellulose citrate: chitosan, (1:1 w/w)Aerogel foams
[142]Chitosan: celluloseSponges

Table 4.

Biocomposites for medical applications.

The films formation by mixtures of cellulose and chitosan in the presence of ionic liquid has shown a successful miscibility in the solid state [139].

The characterization of bacterial cellulose compounds added with xyloglucan (10, 20 and 30 wt%) by means of drying techniques, show that the inclusion of XG promotes better fiber adhesion and orientation [122].

The addition of plasticizers such as sorbitol, glycerol, urea and polyethylene glycol has been studied in order to facilitate thermal processes and to improve the mechanical properties of xyloglucan films. Thus, the results have shown considerable thermal stability as well as strength and hardness for the xyloglucan and sorbitol (20–30%) combination [110].

Results on the films characterization from xyloglucan extracted from Salvia Hispanica L. seeds added with glycerol (25, 50, 75% w/w) indicate that the moisture content, water vapor permeability and solubility of the film in water increased with increasing plasticizer. A high solubility could be advantageous in various applications such as transporter of bioactive compounds [106].

The addition of cross-linking agents such as epichlorohydrin (ECH) for the films preparation of hemicellulose and chitosan (0.1%) involves the reaction of ECH with the chitosan hydroxyl groups. As a result, the smooth, homogeneous and porous surfaces of the obtained films can be beneficial for the breathing of the skin [2].

In the studies on the hydrogels preparation for various applications have proposed the integration of oxidized xyloglucan in combination with chitosan solutions 1% (w/v), the results have evidenced that the strength of the gel depends on the concentration of both polymers [128].

Some researchers have used hemicellulose extracted from woods in combination with chitosan and cross-linked with glutaraldehyde for the hydrogels formation, in consequence the results showed a high response to swelling with increasing hemicellulose content [121].

In the hydrogels preparation studies for the regeneration of osseous tissue, xylan hemicellulose has been incorporated to improve the chitosan properties and was found improvement in the healing of tibia fractures caused by blows, the films preparation was achieved with chitosan:xylan (3:1) mixtures [140].

Researchers have proposed the use of xyloglucan as a protective agent for controlled release of drugs that are limited by the pH of the stomach (pH~1.2), for this, mixtures of xyloglucan (0.5% and 3%) and Enalaprilat (Enal) as pharmaceutical component have been implemented. The results suggest that formulations with 3% xyloglucan can be used as slow drug releaser, especially when needed in other parts of the intestinal tract [141].

The incorporation of carboxylic acid groups by the reaction with citric acid, followed by chitosan addition, has shown an improvement in the properties of sponge-like products, with regard to its elasticity, softness, durability and high porosity [96].

The use of chitosan microparticles to reinforce cellulose biocomposites prepared by lyophilization has been studied, researchers found a more uniform pore size distribution, additionally increasing the chitosan concentration from 0.0 to 1.0% improves the sponge’s resistance to breakage, also an antibacterial behavior against S. aureus and E. coli is exhibited [142].

Xyloglucans show no activity as a bacterial growth inhibitor. In contrast, chitosan exhibits activity against a broad spectrum of microorganisms with absence and absence of hemolytic activity, so it can be potentially applied in the health industry [101].

Assessments of antimicrobial activity of xyloglucan hydrogels from Tamarindus indica seeds and chitosan did not show growth of microorganisms on nutrient agar plates exposed to air pollution [128].

Studies on the biological properties of hemicellulose obtained Prunus amygdalus showed that it could be a promising component to replace synthetic antioxidants [123].

Some alternatives for the conjugates preparation involve the xyloglucan dissolution in chitosan solutions in oil bath for the heat reaction [60]. Other published methods involve the dissolution of hemicellulose xylan, chitooligomers and glucosamine hydrochloride in distilled water, after adjusting the pH with NaOH (1 M) in an oil bath at 100°C for 4 h [143]. The previous oxidation of xyloglucan with sodium periodate has been proposed for the preparation of complex with chitosan to form hydrogels [128]. In other investigations, the addition of hemicellulose to chitosan solutions is carried out in a water bath at 60°C during the preparation of conjugates [2].

Although it is known that cellulose by itself does not possess antimicrobial activity to prevent infection in wounds [81]. Cellulose and its derivatives have been used extensively in combination with chitosan for the preparation of new materials with antimicrobial activity. Some studies report that cellulose and chitosan can be bound by intermolecular inclusion interaction and also based on their antimicrobial capacity against Escherichia coli (Gram−) and Staphylococcus aureus (Gram+), such materials can be used as wound coverings, due to their potential to prevent excessive dehydration and wound infection [95]. Similar studies on the antimicrobial activity of xyloglucan-chitosan hydrogels exposed to atmospheric contamination showed no growth of microorganisms on nutritive agar [128]. Studies on the characterization of chitosan microparticles to reinforce cellulose biocomposites showed antimicrobial activity against Escherichia coli and Staphylococcus aureus with an average zone of inhibition>2 mm and an inhibition rate greater than 80% [142].

3.2. Interaction between functional groups

In hydrogels based on polysaccharides the term “cruising zone” is used to describe crosslinking, because each aggregate involves molecular chains in the form of helices. Generally, the helices are united by non-covalent bonds such as hydrogen bonds, hydrophobic interactions, ionic bonds, etc. [128].

The molecular interaction of both polymers, chitosan and xyloglucan, results in the improvement of hydrogel properties. It is known that cellulose derivatives can act as reinforcement to improve the mechanical and thermal barrier properties. Chitosan by itself has no mechanical and barrier properties [95]. Some factors that influence the combination of both polymers for the formulation of hydrogels is the order of the addition, the concentration and molecular weight of both polymers, temperature, pH and ionic strength of the medium in which they are immersed.

The process for the formation of hydrogels from chitosan and xyloglucan involves the previous dissolution of chitosan in acid solutions, and xyloglucan in distilled water at elevated temperatures and under continuous agitation to prepare the mixtures of both solutions [121]. Other proposed methods involve the oxidation of xyloglucan with periodate prior to the mixing of both polymers [128].

The molecular structure from chitosan and the main chain of xyloglucans are very similar, the difference is the functional group bonded to carbon two in both carbohydrate. Recent research has reported that the addition of hemicelluloses in chitosan polymer biomaterials increases crystallinity and water retention capacity, especially at low pH [135].

Crystallinity occurs because the hemicellulose is capable of interacting with the bonds formed by the chitosan molecules. The ability to retain water is improved by increasing the concentration of hemicellulose increases the amount of hydrogen bonds, which favors the absorption of water. A graphical representation of the physical interaction between chitosan and xyloglucan is shown in Figure 3.

Figure 3.

Graphical representation of physicochemical treatments involved in chitosan and xyloglucan extractions.

Studies on the characterization of hemicellulose and chitosan hydrogels report that the FTIR analyzes showed deformation in the amide II bands and stretching between the OH and NH groups. This is due to the intermolecular interactions between hemicellulose and chitosan, this could be attributed to events such as hydrogen bonds and hydrophobic attractions. Some ionic interactions could take place between carboxyl groups in hemicelluloses and free amino groups in chitosan, although the presence of carboxyl groups in hemicellulose is relatively low [121, 139, 144].

In research on the properties of hemicellulose hydrogels from aspen wood and their interactions with chitosan solutions, it is concluded that the stability of films and hydrogels formation is attributed to the crystalline arrangements and electrostatic interactions of the acidic groups in the hemicellulose and the amino groups in the chitosan [120].

Hydrogels are capable of transforming into a variety of physical forms including slabs, membranes, waits, microspheres, microgels, nanoparticles and porous materials once they have been lyophilized [81]. The biological properties of chitosan and xyloglucan allow the formation of conjugates that transformed into sponges by the process of lyophilization allow obtaining flexible, porous materials and with ability to absorb large amounts of physiological fluids. Properties that make them suitable for biomedical applications in the preparation of coatings [150].

3.3. Mechanical stability

A biocomposite for the restoration of biological tissue based on repair and/or regeneration strategies must meet criteria such as: (a) force to resist application manipulation, (b) biocompatibility with natural polymers, (c) defined structure at the micro-molecular and macromolecular levels and (d) deformation recovery capacity without fracture [74].

Researchers have proposed numerous strategies to promote the chitosan stability and the materials based on this compound. The stability of the chitosan mixtures depends on specific interactions such as hydrogen bonds, ionic bonds, dipole interference; finally, the final properties depend on the miscibility of its components [95]. The addition of plasticizers is necessary to improve the mechanical and permeability properties of some polymer matrices. Such properties could be attributed to the lubricating action to reduce the frictional forces between the chains of the polymers [135].

Plasticizers are usually small molecules that have been employed to increase flexibility and improve the handling of polymeric films. Among plasticizers, glycerol is one of the most widely applied in the films elaboration. It has been successfully introduced in the production of films based on polysaccharides. Features such as water solubility, polarity, non-volatility, and low molecular weight have converted glycerol into a plasticizer compatible with water-soluble polymers (Dick et al. 2015).

The addition of 20% of glycerol to the chitosan films causes the reduction in the tension modulus and the increase in the values of elongation at break [19]. The addition of sorbitol (20–30%) in combination with XG results in a considerable thermal stability superior than that of other plastics, which have disadvantages by evaporation or decomposition at high temperatures [110].

Researchers implemented a methodology for the hydrogel elaboration with a high degree of resistance, through the combination of extracts of bamboo hemicellulose (Phyllostachys pubescens), polyvinyl alcohol (PVA), and chitin nano-cylinders, this methodology allows to associate their molecular chains through physical cross-linking. The polymer segments guarantee the connectivity around the porous membrane, while the water fills the pores and acts as a swelling agent forming hydrogen bonds between the hydroxyl groups of the three polymers [145].

Investigations suggest that the mechanical properties of microporous membranes based on chitosan and hemicellulose are related to the concentration of the cross-linking agent and the reaction temperature, because the increase in the concentration of epichlorohydrin (ECH) during the preparation of membranes results in an increase in tensile strength [2].

3.4. In vivo and in vitro assays

Skin lesions are a common problem affecting the world’s population. Traditionally, the normal healing process is divided into several systematic, and coordinated [2] but overlapping phases: hemostasis/coagulation, inflammation, proliferation (granulation tissue formation), re-epithelialization and remodeling [146, 147]. Usually wounds are classified as wounds from trauma, abrasion or secondary events, wounds without tissue loss, and wounds with tissue loss such as burns [148].

Burn wounds are one of the most complex and painful conditions to treat and handle [56]. These lesions are highly susceptible to infection mainly due to the deterioration of the skin, which acts as a protective barrier against microorganisms [54].

The safety and effectiveness of medical devices made of resorbable biomaterials depends largely on its complete biocompatibility [149]. That is, the tissues and the human body accept the implantable material completely and do not provoke a massive immune response as a result of a threatening material.

3.4.1. Mixture of both polymers

Guan et al. [2] carried out tests on cell viability with films made of hemicellulose—chitosan, and found to exhibit drug-loading capability, non-toxicity, and good compatibility for wound healing application. In addition, the films showed good mechanical properties, uniform porous structure and adequate optical transparency. The trial demonstrates the potential use of natural polymers for the production of films with future perspectives in the biomedical area.

Bush et al. [140] proposed the addition of xylan to chitosan hydrogels to improve restoration of bone fractures in mice. The hydrogels were injected into the site of the lesion. The differences between treatments of chitosan and chitosan with xylan were presented between the 3rd and 4th week. Chitosan hydrogels added with xylan revealed improvement in healing and reduction in fracture size. The untreated fracture was maintained without union after 6 weeks. The above demonstrates that the composite is capable of accelerating the normal healing process in severe wounds, without the need to add growth factors.

3.4.2. Without xyloglucan

One of the essential features that is considered for medical devices is the biocompatibility with the host cells, the anterior is to reduce collateral damage. Biomaterials must accomplish specific requirements in relation to their interactions with the blood elements, thus the materials should not induce coagulation or thrombus formation. Studies on hemocompatibility have established that the lower the value of the hemolysis ratio, the better the biomaterial compatibility with blood. Some authors have reported that a value of up to 5% hemolysis is permissible for biomaterials. Investigations on the in vitro evaluation of chitosan-based nano-materials have shown hemolysis values at 1.14% after 60 min of the material contact with the blood [58].

Investigations in relation to the homeostatic mechanism of biomaterials, through the reaction with erythrocytes, have compared and tested the conventional dressing gauze, polyurethane sponges, chitosan absorbents and aqueous chitosan solution [150]. Experimental studies showed a change in the shape of erythrocytes in contact with conventional gauze and polyurethane sponges, this can be attributed to the morphological changes of the red blood cells by external stimuli; however, no aggregation was observed. Nevertheless, when the erythrocytes were in contact with the porous chitosan sponges showed deformation and its incorporation on the surface of the chitosan sponges, as a result of a hemostatic interaction. The coagulation of blood cells by the chitosan action could be the result of the interaction of the polymer positive charges with the receptors on the cell surface of the erythrocytes.

Lysozyme is present in the human body as body fluid and in tissues, and hydrolyzes the glycosidic bonds (1–4) of chitin and some peptidoglycans. Studies on the in vitro biodegradation of chitosan: gelatin dressings with lysozyme, suggested a total incubation time of 8 days at 37°C [151]. Researchers observed biodegradation of the prototype by 28% after day 8, through the evident destruction of the structure. Studies on the enzymatic activity in lyophilized chitosan-collagen hydrogels exhibited a dynamic degradation from day 14 [152], the anterior could be attributed to the hydrophilicity in sponges that consequently causes the biodegradation and cleavage of the bonds after the swelling of the matrix.

Burns can be classified according to their degree of severity as wounds with loss of tissue and wounds without loss of tissue. To evaluate the curative activity in second degree burns, chitosan coatings were used in rat assays. The coating was replaced every 2 days in the inflammatory phase, 3–4 days in the proliferative phase and every 4–7 days during the maturation phase [153]. It was observed that 90% of the burn healing was reached between the 9th and 12th day. Similar results were obtained in a study where chitosan dressings were loaded with antibiotics. A reduction in wound size was observed between 80 and 90%, with no significant difference on the 15th day after wound induction [154].

To evaluate the efficiency in the quality of cicatrization, female pigs were used for the application of chitosan hydrogel as a coating for third degree burns. Dermal-epidermal reconstruction and re-epithelialization of the affected area were observed without irritation or noxious effects. After 10 months of the cutaneous condition [155], it was observed that the quality of healing, especially in thickness, was better with chitosan hydrogels than with commercial gauze.

Histological observations on the effect of chitosan, heparin and mixtures of both on partial depth burns in adult rats, indicate that burns with local application of chitosan powder are much less severe than control wounds [156]. It also was observed that the mixture of chitosan and heparin inhibited the inflammatory reaction.

Studies on the speed and effectiveness of second degree burns healing in rabbits using chitosan gel, microscopically confirmed the acceleration of wound healing on the 20th day, as a consequence of the epidermal cells proliferation with complete re-epithelialization of the affected area [157].

Clinical studies reported on the healing of human wounds with chitosan-based materials are the result of previous observations in animals. The use of chitosan membranes to cover fresh wounds as a result of a skin graft donor site [53], reported that the wound adherence was uniform, which is a requirement for a successful biomaterial. It was concluded that chitosan membranes promote hemostasis, healing and rapid re-epithelialization of the affected area, transforming it into new, healthy and esthetically acceptable tissue.

Studies on the development and characterization of chitosan-based microparticles added with Aloe Vera and vitamin E, incorporated into a gel for the burns treatment showed mucoadhesive properties influenced by the presence of chitosan. A high degree of re-epithelialization was found after 14 days of treatment [56].

Researchers proposed combining sodium alginate, compatible biopolymer, with chitosan for the films production, this with the aim of improving the skin burn healing in rats as a research model. They concluded that the combination of low level laser therapy with a film based on chitosan and alginate improves the healing process, specifically with respect to re-epithelialization and supply of blood vessels [134].

Chitosan hydrogels with gelatin and honey have been used as coatings for wounds caused by second degree burns in rabbits as an experimental unit. A positive synergistic effect was found with the chitosan and honey mixture in terms of antibacterial activity. Thus, the primary objective in the treatment of burns is achieved, which involves preventing infection and acting as an effective promoter of wound healing caused by burns [45].

Magnesium meets various characteristics to be used as a candidate for applications in biodegradable implants. However, some studies have shown that the rate of magnesium corrosion is too rapid in body fluid to meet medical application characteristics. To counteract the effect, researchers have applied chitosan as an effective corrosion resistant coating, reducing the hydrogen released by the corrosion of the magnesium matrix [158].

Researchers developed nanoparticles of encapsulated insulin as an oral delivery system, which were administered in diabetic rats. The study concluded that oral administration of nanoparticles could be a promising tool to counteract adverse reactions, which are associated with subcutaneous insulin application [159].

3.4.3. Without chitosan

The effectiveness of a polymeric biomaterial for cell restoration depends on the binding of the cells to their surface. In evaluations of in vitro cell adhesion of animal fibroblasts on films with xyloglucan, values of 83% in cell adhesion to membranes have been reported [160]. Due to the anterior, the cell adhesion of the coatings determines its potential biotechnological application in biocompatible wounds.

Recent investigations have focused on the incorporation of growth factors (FGF-18) into injectable xyloglucan gels to promote cartilage reconstruction [161]. These authors report that the porosity and mechanical properties of the gels are highly dependent on the concentration of the polymer, in addition, they exhibit a weight loss between 15 and 20%, followed by a slow disintegration, with increase in rheological properties and porosity. Also, they demonstrated that growth factors are not released by the gel, so that the uncontrolled growth of cartilage in healthy areas is avoided. The cell viability of chondrocytes in xyloglucans with growth promoters involved a suitable environment to grow and proliferate.

In vitro studies with 3% (w/v) xyloglucan covered nanocomposites for the transport of drugs (Enalaprilate) through the gastrointestinal tract, report that these formulations could be used for slow release of drugs when needed in other regions of the gastrointestinal tract [141].

Researchers developed and evaluated xyloglucan-based ocular films as a possible antibiotic-releaser agent such as ciprofloxacin. They performed an eye irritation test on rabbits, in order to determine their ability to cause damage to the cornea, and an acceptable tolerance was reported, with no redness or inflammation. Therefore, ocular administration of xyloglucan is suggested because of the potential absence of irritation [162].

In studies on the biological characterization of xyloglucan extracted from seeds of Hymenaea courbaril var., an evaluation was made on its hemolytic activity. In the essay, the red blood cells were diluted with saline solution to a 1% (v/v) suspension, then was mixed with a xyloglucan solution. The researchers concluded that the polysaccharide has no hemolytic activity, verifying its potential application in the health industry [101].

Tests on the application of cellulose membranes in patients with burn injuries showed that cellulose offers advantages over conventional treatments such as the use of gauze with Vaseline. Total re-epithelialization occurred in about 7 days [163]. The rate of re-epithelialization is closely related to the age of the patient. The healing process slows down with aging.

Some other important properties of xyloglucan include non-carcinogenicity, mucoadhesivity, biocompatibility and high thermal stability. Therefore, researchers have used it for the preparation of microspheres and the encapsulation of anti-asthmatic agents. In vitro pulmonary pharmacokinetic evaluation indicated the potential use of xyloglucan as a release of anti-asthmatic agents through the pulmonary route [164].


4. Conclusion

The review presented in this chapter shows studies on the effectiveness of biomaterials for medical application based on chitosan and xyloglucan, promising results for application in tissue restoration are reported because of their multiple biological properties. Xyloglucans of various sources are defined as a promising biopolymer for biomedical purposes because of their ability to form gels, biocompatibility, adhesiveness, non-carcinogenicity and compatibility with blood. On the other hand, chitosan has remarkable characteristics such as biocompatibility, biodegradability, non-toxicity, bioadhesivity, antigenic capacity and hemostasis. According to the published in vivo and in vitro tests, both polymers could be used for the hydrogels preparation and their application for the wounds with a high level of dehydration to improve their ability to re-epithelialize. In addition, the interaction of xyloglucan with chitosan confers on these biomaterials the antimicrobial capacity over a wide range of pathogenic microorganisms that cause infection. Additionally, the introduction of xyloglucan favors the characteristics of fluid absorption and mechanical resistance in chitosan hydrogels.



The first author is grateful to CONACYT (477730). This research was financed under Project No. 248160 from CONACYT-PN2014 and by Project PROFAPI No. 2017-0010 from Instituto Tecnológico de Sonora.


  1. 1. Baysal K, Aroguz A, Adiguzel Z, Baysal B. Chitosan/alginate crosslinked hydrogels: Preparation characterization and application for cell growth purposes. International Journal of Biological Macromolecules. 2013;59:342-348. DOI: 10.1016/j.ijbiomac.2013.04.073
  2. 2. Guan Y, Qi M, Chen G, Peng F, Sun C. Facile approach to prepare drug-loading film from hemicelluloses and chitosan. Carbohydrate Polymers. 2016;153:542-548. DOI: 10.1016/j.carbpol.2016.08.008
  3. 3. Brodnjak U, Svetec D. Preparation and characterization of chitosan coatings onto regular celulose fibers with ultrasound technique. Journal of Coating Technology and Research. 2013;10(2):247-254. DOI: 10.1007/s11998-012-9447-1
  4. 4. Baouche N, Elchinger P, Baynast H, Pierre G, Delattre C, Michaud P. Chitosan as an adhesive. European Polymer Journal. 2014;60:198-212. DOI: 10.1016/j.eurpolymj.2014.09.008
  5. 5. Hamed I, Özogul F, Regenstein M. Industrial applications of crustacean by-products (chitin, chitosan and chitooligosaccharides): A review. Trends in Food Science and Technology. 2016;48:40-50. DOI: 10.1016/j.tifs.2015.11.007
  6. 6. Moura I, Dias M, Leal C, Carvalho L, de Sousa C, Carvalho E. Chitosan-based dressings loaded with neurotensin-na efficient strategy to improve early diabetic wound healing. Acta Biomaterialia. 2014;10:843-857. DOI: 10.1016/j.actbio.2013.09.040
  7. 7. Hafdani N, Sadeghinia N. A review on application of chitosan as a natural antimicrobial. World Academy of Science, Engineering and Technology. 2011;50:252-256
  8. 8. Cuong H, Minh N, Hoa N, Trung T. Preparation and characterization of high purity β-chitin from squid pens (Loligo chenisis). International Journal of Biological Macromolecules. 2016;93:442-447. DOI: 10.1016/j.ijbiomac.2016.08.085
  9. 9. Wasko A, Bulak P, Polak-Berecka M, Nowak K, Polakowski C, Bieganowski A. The first report of the physicochemical structure of chitin isolated from Hermetia illucens. International Journal of Biological Macromolecules. 2016;92:316-320. DOI: 10.1016/j.ijbiomac.2016.07.038
  10. 10. Erdogan S, Kaya M. High similarity in physicochemical properties of chitin and chitosan from nymphs and adults of a grasshopper. International Journal of Biological Macromolecules. 2016;89:118-126. DOI: 10.1016/j.ijbiomac.2016.04.059
  11. 11. Duarte M, Ferreira M, Marvão M, Rocha J. An optimised method to determine the degree of acetylation of chitin and chitosan by FTIR spectroscopy. International Journal of Biological Macromolecules. 2002;31:1-8. DOI: 10.1016/S0141-8130(02)00039-9
  12. 12. Tan T, Chin H, Tsai M, Liu C. Structural alterations, pore generation, and deacetylation of α- and β- chitin submitted to steam explosion. Carbohydrate Polymers. 2015;122:321-328. DOI: 10.1016/j.carbpol.2015.01.016
  13. 13. Birolli G, de Moura D, Campana F. Ultrasound-assisted conversion of aplha-chitin into chitosan. Applied Acoustics. 2016;103:239-242. DOI: 10.1016/j.apacoust.2015.10.002
  14. 14. Younes I, Rinaudo M. Chitin and chitosan preparation from marine sources. Structure, properties and applications. Marine Drugs. 2015;13(3):1133-1174. DOI: 10.3390/md13031133
  15. 15. Hajji S, Younes I, Ghorbel-Bellaaj O, Hajji R, Rinaudo M, Nasri M, et al. Structural differences between chitin and chitosan extracted from three different marine sources. International Journal of Biological Macromolecules. 2014;65:298-306. DOI: 10.1016/j.ijbiomac.2014.01.045
  16. 16. Badawy M, Rabea E. A biopolimer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. International Journal of Carbohydrate Chemistry. 2011. Article ID: 460381:29. DOI: 10.1155/2011/460381
  17. 17. Lago M, Rodríguez Q, Sendón R, Sanches S, Costa H, Sánchez M, et al. Copilation of analytical methods to characterize and determine chitosan, and main applications of the polymer in food active packaging. Journal of Food Science. 2011;9(4):319-328. DOI: 10.1080/19476337.2011.603844
  18. 18. Babu R, O’Connor K, Seeram R. Current progress on bio-based polymers and their future trends. Progress in Biomaterials. 2013;2:8. DOI: 10.1186/2194-0517-2-8
  19. 19. Rodríguez N, Santana M, Sanchez M, López C, Soto V. Chitosan/hydrophilic plasticizer-based films: Preparation, physicochemical and antimicrobial properties. Journal of Polymers and the Environment. 2014;22:41-51. DOI: 10.1007/s10924-013-0621-z
  20. 20. Tomihata K, Ikada Y. In vitro and in vivo degradation of films of chitin and its deacetylated derivatives. Biomaterials. 1997;18:567-575. DOI: 10.1016/S0142-9612(96)00167-6
  21. 21. Ahmed S, Ikram S. Chitosan based scaffolds and their applications in wound healing. Achievements in the Life Sciences. 2016;10:27-37. DOI: 10.1016/j.als.2016.04.001
  22. 22. Tsigos I, Martinou A, Kafetzopoulos D, Bouriotis V. Chitin deacetylases: New, versatile tools in biotechnology. Trends in Biotechnology. 2000;18(7):305-312. DOI: 10.1016/S0167-7799(00)01462-1
  23. 23. Feng Y, Kopplin G, Kimihiko S, Draget K, Vårum K. Alginate gels with a combination of calcium and chitosan oligomer mixtures as crosslinkers. Carbohydrate Polymers. 2016;156:490-497. DOI: 10.1016/j.carbpol.2016.09.006
  24. 24. Zhang H, Mi J, Hou T, Huang X, Xing J, Yamamoto A, Gao Y. Absorption enhancing effects of chitosan oligomers on the intestinal absorption of low molecular weight heparin in rats. International Journal of Pharmaceutics. 2014;466:156-162. DOI: 10.1016/j.ijpharm.2014.03.010
  25. 25. Dash M, Chiellini F, Fernandez G, Piras M, Chiellini E. Statistical approach to the spectroscopic determination of the deacetylation degree of chitins and chitosans. Carbohydrate Polymers. 2011;86:65-71. DOI: 10.1016/j.carbpol.2011.04.010
  26. 26. Beil S, Schamberger A, Naumann W, Machill S, Van Pée K. Determination of the degree of N-acetylation (DA) of chitin and chitosan in the presence of water by first derivative ATR FTIR spectroscopy. Carbohydrate Polymers. 2012;87:117-122. DOI: 10.1016/j.carbpol.2011.07.025
  27. 27. El Knidri H, El Khalfaoury R, Laajeb A, Addaou A, Lahsini A. Eco-friendly extraction and characterization of chitin and chitosan from the shrimp shell waste via microwave irradiation. Process Safety and Environmental Protection. 2016;104:395-405. DOI: 10.1016/j.psep.2016.09.020
  28. 28. Fiamingo A, de Moura D, Trombotto S, David L, Campana F. Extensively deacetylated high molecular weight chitosan from the multistep ultrasound-assisted deacetylation of beta-chitin. Ultrasonics Sonochemistry. 2016;32:79-85. DOI: 10.1016/j.ultsonch.2016.02.021
  29. 29. Sayari N, Sila A, Abdelmalek B, Abdallah R. Chitin and chitosan from the Norway lobster by-products: Antimicrobial and anti-proliferative activities. International Journal of Biological Macromolecules. 2016;87:163-171. DOI: 10.1016/j.ijbiomac.2016.02.057
  30. 30. Tokuyasu K, Ono H, Ohnishi K, Hayashi K, Mori Y. Deacetylation of chitin oligosaccharides of dp 2-4 by chitin deacetylase from Colletotrichum lindemuthianum. Carbohydrate Research. 1997;303:353-358. DOI: 10.1016/S0008-6215(97)00166-3
  31. 31. Martinou A, Bouriotis V, Stokke T, Vårum M. Mode of action of chitin deacetylase from Mucor rouxii on partially N-acetylated chitosans. Carbohydrate Research. 1998;31:71-78. DOI: 10.1016/S0008-6215(98)00183-9
  32. 32. Aguila E, Gomes L, Andrade C, Silva J, Paschoalin V. Biocatalytic production of chitosan polymers from shrimp shells, using a recombinant enzyme produced by Pichia pastoris. American Journal of Molecular Biology. 2012;2:341-350. DOI: 10.4236/ajmb.2012.24035
  33. 33. Ikeda M, Kondo Y, Matsumiya M. Purification, characterization, and molecular cloning of chitinases from the stomach of the threeline grunt Parapristipoma trilineatum. Process Biochemistry. 2013;48:1324-1334. DOI: 10.1016/j.procbio.2013.06.016
  34. 34. Jaworska M. Kinetics of enzymatic deacetylation of chitosan. Cellulose. 2012;19:363-369. DOI: 10.1007/s10570-012-9650-3
  35. 35. Villa L, Gonzáles M, Gimeno M, López L, Bárzana E, Shirai K. Ultrasonication and steam-explosion as chitin pretreatments for chitin oligosaccharide production by chitinases of Lecanicillium lecanni. Bioresource Technology. 2013;146:794-798. DOI: 10.1016/j.biortech.2013.08.003
  36. 36. Pinheiro A, Bourbon A, Cerqueira M, Maricato E, Nunes C, Coimbra M, et al. Chitosan/fucoidan multilayer nanocapsules as a vehicle for controlled release of bioactive compounds. Carbohydrate Polymers. 2015;115:1-9. DOI: 10.1016/j.carbpol.2014.07.016
  37. 37. Bansal V, Sharma P, Sharma N, Pal O, Malviya R. Applications of chitosan and chitosan derivatives in drug delivery. Advanced Biomedical Research. 2011;5:28-37
  38. 38. Liu L, Tang X, Wang Y, Guo S. Smart gelation of chitosan solution in the presence of NaHCO3 for injectable drug delivery system. International Journal of Pharmaceutics. 2011;414:6-15. DOI: 10.1016/j.ijpharm.2011.04.052
  39. 39. Onuki Y, Bhardwaj U, Papadimitrakopoulos F, Burgess D. A review of the biocompatibility of implantable devices: Current challenges to overcome foreign body response. Journal of Diabetes Science and Technology. 2008;2(6):1003-1015
  40. 40. Anderson J, Shive M. Biodegradation and biocompatibility of PLA and PLGA microspheres. Advanced Drug Delivery Reviews. 2012;28:5-24. DOI: 10.1016/S0169-409X(97)00048-3
  41. 41. Lee J, Park J, Robinson J. Bioadhesive-based dosage forms: The next generation. Journal of Pharmaceutical Sciences. 2000;89:850-866. DOI: 10.1002/1520-6017(200007)89:7<850::AID-JPS2>3.0.CO;2-G
  42. 42. Kang P, Chang S, Manousakas I, Lee C, Yao C, Lin F, et al. Development and assessment of hemostasis chitosan dressings. Carbohydrate Polymers. 2011;85:565-570. DOI: 10.1016/j.carbpol.2011.03.015
  43. 43. Rao S, Sharma C. Use of chitosan as a biomaterial: Studies on its safety and hemostatic potential. Journal of Biomedical Materials Research. 1997;34:21-28. DOI: 10.1002/(sici)1097-4636(199701)34:160;21::aid-jbm462;;2-p
  44. 44. Lan G, Lu B, Wang T, Wang L, Chen J, Yu K, et al. Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent. Colloids and Surfaces B. 2015;136:1026-1034. DOI: 10.1016/j.colsurfb.2015.10.039
  45. 45. Wang T, Zhu X, Xue X, Wu D. Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings. Carbohydrate Polymers. 2012;88:75-83. DOI: 10.1016/j.carbpol.2011.11.069
  46. 46. Mora V, Velasco D, Hernández R, Mijangos C, Kumacheva E. Chitosan/agarose hydrogels: Cooperative properties and microfluidic preparation. Carbohydrate Polymers. 2014;111:348-355. DOI: 10.1016/j.carbpol.2014.04.087
  47. 47. Ong S, Wu J, Moochhala S, Tan M, Lu J. Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties. Biomaterials. 2008;29:4323-4332. DOI: 10.1016/j.biomaterials.2008.07.034
  48. 48. Luckachan G, Pillai C. Biodegradable polymers- a review on recent trends and emerging perspectives. Journal of Polymers and the Environment. 2011;19:637-676. DOI: 10.1007/s10924-011-0317-1
  49. 49. Huang J, Shetty A, Wang M. Biodegradable plastics: A review. Advances in Polymer Technology. 1990;10:23-30. DOI: 10.1002/adv.1990.060100103
  50. 50. Berger J, Reist M, Mayer J, Felt O, Gurny R. Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications. European Journal of Pharmaceutics and Biopharmaceutics. 2004;57:35-52. DOI: 10.1016/S0939-6411(03)00160-7
  51. 51. Adekogbe I, Ghanem A. Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering. Biomaterials. 2005;26:7241-7250. DOI: 10.1016/j.biomaterials.2005.05.043
  52. 52. Wang H, Chou Y, Wen Z, Wang Z, Chen C, Ho M. Novel biodegradable porous scaffold applied to skin regeneration. PLoS One. 2013;8(6):e56330. DOI: 10.1371/journal.pone.0056330
  53. 53. Azad K, Sermsintham N, Chandrkrachang S, Stevens F. Chitosan membrane as a wound-healing dressing: Characterization and clinical application. Journal of Biomedical Materials Research. 2004;69B(2):216-222. DOI: 10.1002/jbm.b.30000
  54. 54. Sohrabi S, Haeri A, Mahboubi A, Mortazavi A, Dadashzadeh S. Chitosan-gel-embedded moxifloxacin niosomes: An efficent antimicrobial hybrid system for burn infection. International Journal of Biological Macromolecules. 2016;85:625-633. DOI: 10.1016/j.ijbiomac.2016.01.013
  55. 55. Baxter R, Dai T, Kimball J, Wang E, Hamblin M, Wiesmann W, et al. Chitosan dressing promotes healing in third degree burns in mice: Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling. Journal of Biomedical Materials Research. Part A. 2013;101(2):340-348. DOI: 10.1002/jbm.a.34328
  56. 56. Pereira G, Oliveira R, Albernaz M, Canema D, Weismüller G, Barros E, et al. Microparticles of Aloe vera/vitamin E/chitosan: Microscopic a nuclear imaging and an in vivo test analysis for burn treatment. European Journal of Pharmaceutics and Biopharmaceutics. 2014;86:292-300. DOI: 10.1016/j.ejpb.2013.10.011
  57. 57. Ahamed M, Sastry T. Wound dressing application of chitosan based bioactive compounds. International Journal of Pharmacy and Life Sciences. 2011;2(8):991-996
  58. 58. Archana D, Dutta J, Dutta P. Evaluation of chitosan nano dressing for wound healing: Characterization, in vitro and in vivo studies. International Journal of Biological Macromolecules. 2013;57:193-203. DOI: 10.1016/j.ijbiomac.2013.03.002
  59. 59. Khor E, Lim L. Implantable applications of chitin and chitosan. Biomaterials. 2003;24:2339-2349. DOI: 10.1016/S0142-9612(03)00026-7
  60. 60. Li X, Shi X, Wang M, Du Y. Xylan chitosan conjugate–A potential food preservative. Food Chemistry. 2011;126:520-525. DOI: 10.1016/j.foodchem.2010.11.037
  61. 61. Pereda M, Ponce A, Marcovich N, Ruseckaite R, Martucci J. Chitosan-gelatin composites and bi-layer films with potential antimicrobial activity. Food Hydrocolloids. 2011;25:1372-1381. DOI: 10.1016/j.foodhyd.2011.01.001
  62. 62. Liu R, Xu X, Zhuang X, Cheng B. Solution blowing of chitosan/PVA hydrogel nanofber mats. Carbohydrate Polymers. 2014;101:1116-1121. DOI: 10.1016/j.carbpol.2013.10.056
  63. 63. Anisha B, Biswas R, Chennazhi K, Jayakumar R. Chitosan-hyaluronic acid/nanosilver composites sponges for drug resistant bacterial infected diabetic wounds. International Journal of Biological Macromolecules. 2013;62:310-320. DOI: 10.1016/j.ijbiomac.2013.09.011
  64. 64. Abdelgawad A, Hudson S, Rojas O. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol). Carbohydrate Polymers. 2014;100:166-179. DOI: 10.1016/j.carbpol.2012.12.043
  65. 65. Panczyk M, Staroszczyk H, Gottfried K, Kolodziejska I, Pajak W. Antimicrobial properties of chitosan solutions, chitosan films and gelatin-chitosan films. Polimery. 2015;60:735-741. DOI: 10.14314/polimery.2015.735
  66. 66. Ayoub A, Venditti R, Pawlak J, Salam A, Hubbe A. Novel hemicellulose-chitosan biosorbent for wáter desalination and heavy metal removal. ACS Sustainable Chemistry & Engineering. 2013;1:1102-1109. DOI: 10.1021/sc300166m
  67. 67. Ahmed E. Hydrogel: Preparation, characterization, and applications: A review. Journal of Advanced Research. 2015;6:105-121. DOI: 10.1016/j.jare.2013.07.006
  68. 68. Zhou H, Jiang l CP, Li J, Chen X. Glycerolphosphate-based chitosan thermosensitive hydrogels and their biomedical applications. Carbohydrate Polymers. 2015;117:524-536. DOI: 10.1016/j.carbpol.2014.09.094
  69. 69. Das N, Bera T, Mukherjee A. Biomaterial hydrogels for diferente biomedical applications. International Journal of Pharma and Bio Sciences. 2012;3(3):586-597
  70. 70. Zhu C, Zhao J, Kempe K, Wilson P, Wang J, Velkov T, et al. A hydrogel-based localized release of Colistin for antimicrobial treatment of burn wound infection. Macromolecular Bioscience. 2016;17(2):1600320. DOI: 10.1002/mabi.201600320
  71. 71. Dragan E. Design and applications of interpenetrating polymer network hydrogels. A review. Chemical Engineering Journal. 2014;243:572-590. DOI: 10.1016/j.cej.2014.01.065
  72. 72. Deligkaris K, Tadele T, Olthuis W, Van den Berg A. Hydrogel –based devices for biomedical applications. Sensors and Actuators B: Chemical. 2010;147:765-774. DOI: 10.1016/j.snb.2010.03.083
  73. 73. Caló E, Khutoryanskiy V. Biomedical applications of hydrogels: A review of patents and commercial products. European Polymer Journal. 2015;65:252-267. DOI: 10.1016/j.eurpolymj.2014.11.024
  74. 74. Costa M, Mano F. Extremely strong and tough hydrogels as prospective candidates for tissue repair- a review. European Polymer Journal. 2015;72:344-364. DOI: 10.1016/j.eurpolymj.2015.07.053
  75. 75. Ruiz M, Sastre A, Guinal E. Palladium sorption on glutaraldehyde-crosslinked chitosan. Reactive and Functional Polymers. 2000;45:155-173. DOI: 10.1016/S1381-5148(00)00019-5
  76. 76. Bao D, Chen M, Wang H, Wang J, Liu C, Sun R. Preparation and characterization of double crosslinked hydrogel films from carboxymethylchitosan and carboxymethylcellulose. Carbohydrate Polymers. 2014;110:113-120. DOI: 10.1016/j.carbpol.2014.03.095
  77. 77. Ji Q, Deng J, Xiang X, Yuan C, Yu X, Xu Q, et al. Biocompatibility of a chitosan-based injectable thermosensitive hidrogel and its effects on dog periodontal tissue regeneration. Carbohydrate Polymers. 2010;82:1153-1160. DOI: 10.1016/j.carbpol.2010.06.045
  78. 78. Miguel S, Ribeiro M, Brancal H. Thermoresponsive chitosan-agarose hydrogel for skin regeneration. Carbohydrate Polymers. 2014;111:366-373. DOI: 10.1016/j.carbpol.2014.04.093
  79. 79. Sharma G, Naushad M, Al-Muhtaseb A, Kumar A, Khan M, Kalia S, et al. Fabrication and characterization of chitosan-crosslinked-poly (alginic acid) nanohydrogel for adsorptive removal of Cr (IV) metal ion from aqueous medium. International Journal of Biological Macromolecules. 2017;95:484-493. DOI: 10.1016/j.ijbiomac.2016.11.072
  80. 80. Croisier F, Jérôme C. Chitosan-based biomaterials for tissue engineering. European Polymer Journal. 2013;49:780-792. DOI: 10.1016/j.eurpolymj.2012.12.009
  81. 81. Shen X, Shamshina J, Berton P, Gurau G, Rogers R. Hydrogels based on cellulose and chitin fabrication, properties, and applications. Green Chemistry. 2016;18:53-75. DOI: 10.1039/C5GC02396C
  82. 82. Zeng D, Wu J, Kennedy J. Application of a chitosan flocculant to water treatment. Carbohydrate Polymers. 2008;71(1):135-139. DOI: 10.1016/j.carbpol.2007.07.039
  83. 83. Divakaran R, Pillai S. Flocculation of kaolinite suspensions in water by chitosan. Water Research. 2001;35:3904-3908. DOI: 10.1016/S0043-1354(01)00131-2
  84. 84. Ang W, Mohammad A, Benamor A, Hilal N. Chitosan as natural coagulant in hybrid coagulation-nanofiltration membrane process for water treatment. Journal of Environmental Chemical Engineering (JECE). 2016;4(4):4857-4862. DOI: 10.1016/j.jece.2016.03.029
  85. 85. Seyed D, Amani G, Hanifehpour Y, Woo J, Figoli A, Carraro M, et al. Performance of chitosan based nanocomposite hallow fibers in the removal of selenium (IV) from water. Chemical Engineering Research and Design. 2017;117:309-317. DOI: 10.1016/j.cherd.2016.10.043
  86. 86. Rocha L, Almeida A, Nunes C, Henriques B, Coimbra M, Lopes C, et al. Simple and effective chitosan based films for the removal of Hg from waters: Equilibrium, kinetic and ionic competition. Chemical Engineering Journal. 2016;300:217-229. DOI: 10.1016/j.cej.2016.04.054
  87. 87. Yuan G, Chen X, Li D. Chitosan films and coatings containing essential oils: The antioxidant and antimicrobial activity, and application in food systems. Food Research International. 2016;89:117-128. DOI: 10.1016/j.foodres.2016.10.004
  88. 88. Elsabee M, Abdou E. Chitosan based edible films and coatings: A review. Materials Science & Engineering, C: Materials for Biological Applications. 2013;33:1819-1841. DOI: 10.1016/j.msec.2013.01.010
  89. 89. Dong H, Cheng L, Tan J, Zheng K, Jiang Y. Effects of chitosan coating on quality and shelf life of peeled litchi fruit. Journal of Food Engineering. 2004;64:355-358. DOI: 10.1016/j.jfoodeng.2003.11.003
  90. 90. Coma V, Gros A, Garreau S, Copinet A, Salin F, Deschamps A. Edible antimicrobial films based on chitosan matrix. Journal of Food Science. 2002;67:1162-1169. DOI: 10.1111/j.1365-2621.2002.tb09470.x
  91. 91. Leceta I, Guerrero P, Ibarburu I, Dueñas M, de la Caba K. Characterization and antimicrobial analysis of chitosan-based films. Journal of Food Engineering. 2013;116:889-899. DOI: 10.1016/j.jfoodeng.2013.01.022
  92. 92. Bourtoom T, Chinnan M. Preparation and properties of rice starch-chitosan blend biodegradable film. Food Science and Technology. 2008;41:1633-1641. DOI: 10.1016/j.lwt.2007.10.014
  93. 93. Malerba M, Cerana R. Chitosan Effects on Plant Systems. International Journal of Molecular Sciences. 2016;17(7):pii: E996. DOI: 10.3390/ijms17070996
  94. 94. Kashyap P, Xiang X, Heiden P. Chitosan nanoparticle based delivery systems for sustainable agriculture. International Journal of Biological Macromolecules. 2015;77:36-51. DOI: 10.1016/j.ijbiomac.2015.02.039
  95. 95. Abdul Khalil HPS, Saurabh K, Chaturbhuj K, Adnan A, Fazita N, Syakir M, et al. A review on chitosan-cellulose blends and nanocellulose reinforced chitosan biocomposites: Properties and their applications. Carbohydrate Polymers. 2016;150:216-226. DOI: 10.1016/j.carbpol.2016.05.028
  96. 96. Salam A, Venditti R, Pawlak J, El-Tahlawy K. Crosslinked hemicellulose citrate-chitosan aerogel foam. Carbohydrate Polymers. 2011;84:1221-1229. DOI: 10.1016/j.carbpol.2011.01.008
  97. 97. Simi K, Abraham E. Physico chemical properties of aminated tamarind xyloglucan. Colloids and Surfaces, B: Biointerfaces. 2010;81:513-520. DOI: 10.1016/j.colsurfb.2010.07.051
  98. 98. Madgulkar A, Bhalekar M, Asgaonkar K, Dikpati A. Synthesis and characterization of a novel mucoadhesive derivative of xyloglucan. Carbohydrate Polymers. 2016;135:356-362. DOI: 10.1016/j.carbpol.2015.08.045
  99. 99. Hayashi T, Kaida R. Functions of xyloglucan in plant cells. Molecular Plant. 2011;4(1):17-24. DOI: 10.1093/mp/ssq063
  100. 100. Lamothe M, Srichuwong S, Reuhs L, Hamaker R. Quinoa (Chenopodium quinoa W.) and amaranth (Amaranthus caudatus L.) provide dietary fibres high in pectic substances and xyloglucans. Food Chemistry. 2015;167:490-496. DOI: 10.1016/j.foodchem.2014.07.022
  101. 101. Arruda R, Albuquerque B, Santos R, Silva G, Mourão A, Correia T, et al. Structure and rheological properties of a xyloglucan extracted from Hymenaea courbaril var. courbaril seeds. International Journal of Biological Macromolecules. 2015;73:31-38. DOI: 10.1016/j.ijbiomac.2014.11.001
  102. 102. Yan Y, Takemasa M, Zhao C, Yu L, Nishinari K. Structure-gelation research on gallate analogs and xyloglucan by rheology, thermal analysis and NMR. Food Hydrocolloids. 2016;52:447-459. DOI: 10.1016/j.foodhyd.2015.07.012
  103. 103. Guindani C, Podestá R, Block J, Rossi M, Mezzomo N, Ferreira S. Valorization of chia (Salvia hispánica) seed cake by means of supercritical fluid extraction. Journal of Supercritical Fluids. 2016;112:67-75. DOI: 10.1016/j.supflu.2016.02.010
  104. 104. Felisberto F, Wahanik A, Gomes-Ruffi C, Clerici S, Chang Y, Steel C. Use of chia (Salvia hispanica L.) mucilage gel to reduce fat in pound cakes. LWT- Food Science and Technology. 2015;63:1049-1055. DOI: 10.1016/j.lwt.2015.03.114
  105. 105. Timilsena Y, Wang B, Adhikari R, Adhikari B. Preparation and characterization of chia seed protein isolate-chia seed gum complex coacervates. Food Hydrocolloids. 2016;52:554-563. DOI: 10.1016/j.foodhyd.2015.07.033
  106. 106. Dick M, Costa H, Gomaa A, Subirade M, de Olivero R, Flôres H. Edible film production from chia seed mucilage: Effect of glycerol concentration on its physicochemical and mechanical properties. Carbohydrate Polymers. 2015;130:198-205. DOI: 10.1016/j.carbpol.2015.05.040
  107. 107. Salgado C, Calderón D, Chanona P, Ferrera R, Méndez M, Díaz R. Chia (Salvia hispánica L.) seed mucilage release characterisation. A microstructural and image analysis study. Industrial Crops and Products. 2013;51:453-462. DOI: 10.1016/j.indcrop.2013.09.036
  108. 108. Nishinari K, Takemasa M, Yamatoya K, Shirakawa. Xyloglucan. In: Philips GO, Williams PA, editors. Handbook of Hydrocolloids. Woodhead Publishing Limited; 2009. pp. 535-566
  109. 109. Avachat A, Gujar K, Wagh K. Development and evaluation of tamarind seed xyloglucan-based mucoadhesive buccal films of rizatriptan benzoate. Carbohydrate Polymers. 2013;91:537-542. DOI: 10.1016/j.carbpol.2012.08.062
  110. 110. Bergström M, Salmén L, Kochumalayil J, Berglund L. Plasticized xyloglucan for improved toughness-thermal and mechanical behavior. Carbohydrate Polymers. 2012;87:2532-2537. DOI: 10.1016/j.carbpol.2011.11.024
  111. 111. Buckeridge M, Rocha D, Reid G, Dietrich M. Xyloglucan structure and post-germinative metabolism in seeds of Copaidera langsdorfii from savanna and forest populations. Physiologia Plantarum. 1992;86:145-151. DOI: 10.1111/j.1399-3054. 1992.tb01323.x
  112. 112. Stupp T, de Freitas R, Sierakowski M, Deschamps F, Wisniewski J, Biavatti M. Characterization and potential uses of Copaifera langsdorfii seeds and seed oil. Bioresource Technology. 2008;99:2659-2663. DOI: 10.1016/j.biortech.2007.04.051
  113. 113. Tiné S, Cortelazzo L, Silveira B. Xyloglucan mobilisation in cotyledons of developing plantlets of Hymenaea courbarl L. (Leguminosae-Caesalpinoideae). Plant Science. 2000;154:117-126. DOI: 10.1016/S0168-9452(99)00245-9
  114. 114. Alcântara P, Martim L, Silva C, Dietrich S, Buckeridge M. Purification of a β-galactosidase from cotyledons of Hymenaea Courbaril L. (Leguminosae). Enzyme properties and biological function. Plant Physiology and Biochemistry. 2006;44:619-627. DOI: 10.1016/j.plaphy.2006.10.007
  115. 115. Lima N, Quoirin M, Naddaf Y, Wilhelm H, Ribas L, Sierakowski M. A xyloglucan from sedes of the native Brazilian species Hymenaea courbaril for micropropagation of Marubakaido and Jonagored apples. Plant Cell Reports. 2003;21:402-407. DOI: 10.1007/s00299-002-0538-9
  116. 116. Lucyszyn N, Lubambo A, Ono L, Jó T, Souza C, Sierakowski R. Chemical, physic-chemical and cytotoxicity characterisation of xyloglucan from Guibourtia hymenifolia (Moric.) J. Leonard Seeds. Food Hydrocolloids. 2011;25(5):1242-1250. DOI: 10.1016/j.foodhyd.2010.11.012
  117. 117. Wang Q, Ellis P, Ross M, Burchard W. Solution characteristics of the xyloglucan extracted from Detarium senegalense Gmelin. Carbohydrate Polymers. 1997;33(2–3):115-124. DOI: 10.1016/S0144-8617(97)00026-X
  118. 118. Capitani M, Corzo R, Chel G, Betancur A, Nolasco S. Rheological propierties of aqueous dispersions of chia (Salvia hispanica L.). Journal of Food Engineering. 2015;149:70-77. DOI: 10.1016/j.jfoodeng.2014.09.043
  119. 119. Muñoz L, Cobos A, Diaz O, Aguilera J. Chia seed: Microstructure, mucilage extraction and hydration. Journal of Food Engineering. 2012;108:216-224. DOI: 10.1016/j.jfoodeng.2011.06.037
  120. 120. Gabrielii I, Gatenholm P, Glasser W, Jain R, Kenne L. Separation, characterization and hydrogel-formation of hemicellulose from aspen wood. Carbohydrate Polymers. 2000;40:367-374. DOI: 10.1016/S0144-8617(00)00181-8
  121. 121. Karaaslan A, Tshabalala M, Buschle- Diller G. Wood hemicellulose/chitosan-based semi-interpenetrating network hydrogels: Mechanical, swelling and controlled drug reléase properties. BioResources. 2010;5(2):1036-1054
  122. 122. Souza C, Lucyszyn N, Woehl M, Riegel-Vidotti I, Borsali R, Sierakowski M. Property evaluations of dry-cast reconstituted bacterial cellulose/tamarind xyloglucan biocomposites. Carbohydrate Polymers. 2013;93:144-153. DOI: 10.1016/j.carbpol.2012.04.062
  123. 123. Bouaziz F, Koubaa M, Helbert B, Kallel F, Driss D, Kacem I, et al. Purification, structural data and biological properties of polysaccharide from Prunus amygdalus gum. International Journal of Food Science and Technology. 2014;50(3):578-584. DOI: 10.1111/ijfs.12687
  124. 124. Bouaziz F, Koubaa M, Jeddou K, Kallel F, Helbert B, Khelfa A, et al. Water-soluble polysaccharides and hemicelluloses from almond gum: Functional and prebiotic properties. International Journal of Biological Macromolecules. 2016;93:359-368. DOI: 10.1016/j.ijbiomac.2016.08.032
  125. 125. Zhao X, Tong T, Li H, Lu H, Ren J, Zhang A, et al. Characterization of hemicelluloses from Neolamarckia cadamba (Rubiaceae) during xylogenesis. Carbohydrate Polymers. 2017;156(20):333-339. DOI: 10.1016/j.carbpol.2016.09.041
  126. 126. Hanim S, Norsafurah S. Physical properties of hemicellulose film from sugarcane bagasse. Procedia Engineering. 2012;42:1390-1395. DOI: 10.1016/j.proeng.2012.07.532
  127. 127. Mahajan S, Mahajan R. Development of grafted xyloglucan micelles for pulmonary delivery of curcumin: In vitro and in vivo studies. International Journal of Biological Macromolecules. 2016;82:621-627. DOI: 10.1016/j.ijbiomac.2015.09.053
  128. 128. Simi K, Abraham T. Transparent xyloglucan-chitosan complex hydrogels for different applications. Food Hydrocolloids. 2010;24:72-80. DOI: 10.1016/j.foodhyd.2009.08.007
  129. 129. Brun-Graeppi A, Richard C, Bessodes M, Scherman D, Narita T, Ducouret G, et al. Study on the sol-gel transition of xyloglucan hydrogels. Carbohydrate Polymers. 2010;80:555-562. DOI: 10.1016/j.carbpol.2009.12.026
  130. 130. Kai K, Petkowickz C. Influence of extraction conditions on properties of seed xyloglucan. International Journal of Biological Macromolecules. 2010;46:223-228. DOI: 10.1016/j.ijbiomac.2009.12.012
  131. 131. Mahamadi C. Water hyacinth as a biosorbent: A review. African Journal of Environmental Science and Technology. 2011;5(13):1137-1145. DOI: 10.5897/AJESTX11.007
  132. 132. Mishra A. Tamarind seed xyloglucan: A food hydrocolloid for water remediation. Journal of Biobased Materials and Bioenergy. 2013;7:12-18. DOI: 10.1166/jbmb.2013.1282
  133. 133. Roy S, Prabhakar B. Bioadhesive polymeric platforms for transmucosal drug delivery systems-a review. Tropical Journal of Pharmaceutical Research. 2010;9(1):91-104. DOI: 10.4314/tjpr. v9i1.52043
  134. 134. Dantas M, Cavalcante D, Araújo F, Barretto S, Aciole G, Pinheiro A, et al. Improvement of dermal burn healing by combining sodium alginate/chitosan-based films and low level laser therapy. Journal of Photochemistry and Photobiology. B. 2011;105:51-59. DOI: 10.1016/j.jphotobiol.2011.06.009
  135. 135. Hernández A, Bohórquez V. Inclusión de compuestos químicos en matrices polímericas de quitosano y su efecto en las propiedades de película. VITAE, Revista de la facultad de química farmacéutica. 2014;21(1):49-59
  136. 136. Mogosanu G, Grumezescu A. Natural and synthetic polymers for wounds and burns dressing. International Journal of Pharmaceutics. 2014;463:127-136. DOI: 10.1016/j.ijpharm.2013.12.015
  137. 137. Chang C, Duan B, Cai J, Zhang L. Superabsorbent hydrogels based on cellulose for smart swelling and controllable delivery. European Polymer Journal. 2010;46:92-100. DOI: 10.1016/j.eurpolymj.2009.04.033
  138. 138. Gong J, Osada Y. Soft and wet materials: From hydrogels to biotissues. In: Cloitre M, editor. High Solid Dispersions, Advances in Polymer Science. Berlin, Heidelberg: Springer; 2010. pp. 203-246. DOI: 10.1007/12_2010_91
  139. 139. Stefanescu C, Daly H, Negulescu I. Biocomposite films prepared from ionic liquid solutions of chitosan and cellulose. Carbohydrate Polymers. 2012;87:435-443. DOI: 10.1016/j.carbpol.2011.08.003
  140. 140. Bush R, Liang H, Dickinson M, Botchwey A. Xylan hemicellulose improves chitosan hydrogel for bone tissue regeneration. Polymers for Advanced Technologies. 2016;27(8):1050-1055. DOI: 10.1002/pat.3767
  141. 141. Ribeiro C, Arizaga G, Wypych F, Sierakowski M. Nanocomposites coated wih xyloglucan for drug delivery: In vitro studies. International Journal of Pharmaceutics. 2009;367(1–2):204-210. DOI: 10.1016/j.ijpharm.2008.09.037
  142. 142. Lv F, Wang C, Zhu P, Zhang C. Characterization of chitosan microparticles cellulose biocomposite sponges regenerated from ionic liquid. Cellulose. 2014;21:4405-4418. DOI: 10.1007/s10570-014-0440-y
  143. 143. Wu S, Hu J, Wei L, Du Y, Shi X, Zhang L. Antioxidant and antimicrobial activity of maillard reaction products from xylan with chitosan/chitooligomer/glucosamine hydrochloride/taurine model systems. Food Chemistry. 2014;148:196-203. DOI: 10.1016/j.foodchem.2013.10.044
  144. 144. Fangbing L, Wang C, Zhu P, Zhang C. Characterization of chitosan microparticles reinforced cellulose biocomposite sponges regenerated from ionic liquid. Cellulose. 2014;21:4405-4418. DOI: 10.1007/s10570-014-0440-y
  145. 145. Guan Y, Bian J, Peng F, Ming Z, Cang S. High strength of hemicelluloses based hydrogels by freeze/thaw technique. Carbohydrate Polymers. 2014;101:272-280. DOI: 10.1016/j.carbpol.2013.08.085
  146. 146. Tang F, Lv L, Lu F, Rong B, Li Z, Lu B, et al. Preparation and characterization on N-chitosan as a wound healing accelerator. International Journal of Biological Macromolecules. 2016;93(Pt A):1295-1303. DOI: 10.1016/j.ijbiomac.2016.09.101
  147. 147. Moura L, Dias A, Carvalho E, de Sousa H. Recent advances on the development of wound dressings for diabetic foot ulcer treatment- a review. Acta Biomaterialia. 2013;9:7093-7114. DOI: 10.1016/j.actbio.2013.03.033
  148. 148. Paul W, Sahrma C. Chitosan and alginate wound dressigns: A short review. Biomaterials and Artificial Organs. 2004;18(1):18-23
  149. 149. Villa T, Brianza S. Form and function of resorbable materials-based medical devices. In: Perale G, Hilborn J, editors. Bioresorbable Polymers for Biomedical Applications. Woodhead Publishing; 2017. pp. 95-100. DOI: 10.1016/B978-0-08-100262-9.00005-7
  150. 150. Chen C, Liu L, Huang T, Wang Q, Fang Y. Bubble template fabrication of chitosan/poly (vinyl alcohol) sponges for wound dressing applications. International Journal of Biological Macromolecules. 2013;62:188-193. DOI: 10.1016/j.ijbiomac.2013.08.042
  151. 151. Alizadeh M, Abbasi F, Khoshfetrat A, Ghaleh H. Microstructure and characteristic properties of gelatin/chitosan scaffold prepared by a combined freeze-drying/leaching method. Materials Science & Engineering, C: Materials for Biological Applications. 2013;33:3958-3967. DOI: 10.1016/j.msec.2013.05.039
  152. 152. Arpornmaeklong P, Pripatnanont P, Suwatwirote N. Properties of chitosan-collagen sponges and osteogenic differentiation of rat-bone-marrow stromal cells. International Journal of Oral and Maxillofacial Surgery. 2008;37:357-366. DOI: 10.1016/j.ijom.2007.11.014
  153. 153. Khodja N, Mahlous M, Tahtat D, Benamer S, Youcef L, Chader H, et al. Evaluation of healing activity if PVA/chitosan hydrogels on deep second degree burn: Pharmacological and toxicological test. Burns. 2013;39:98-104. DOI: 10.1016/j.burns.2012.05.021
  154. 154. Sung H, Hwang M, Kim O, Lee H, Kim I, Kim H, et al. Gel characterization and in vivo evaluation of minocycline- loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan. International Journal of Pharmaceutics. 2010;392:232-240. DOI: 10.1016/j.ijpharm.2010.03.024
  155. 155. Boucard N, Viton C, Agay D, Mari E, Roger T, Cancerelle Y, et al. The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials. 2007;28:3478-3488. DOI: 10.1016/j.biomaterials.2007.04.021
  156. 156. Jin Y, Ling X, He Y, Zhang M. Effects of chitosan and heparin on early extension of burns. Burns. 2007;33:1027-1031. DOI: 10.1016/j.burns.2006.12.002
  157. 157. Honardar S, Kordestani S, Daliri M, NayebHabib F. The effect of chitosan-based gel on second degree burn wounds. Journal of Wound Care. 2016;25(8):488-494. DOI: 10.12968/jowc.2016.25.8.488
  158. 158. Liangjian C, Jun Z, Kun Y, Chang C, Yilong D, Xueyan Q, et al. Improving of in vitro biodegradation resistance in a chitosan coated magnesium bio-composite. Rare Metal Materials and Engineering. 2015;44(8):1862-1865. DOI: 10.1016/S1875-5372(15)30114-4
  159. 159. Erel G, Kotmakçı M, Akbaba H, Karadağlı S, Kantaracı A. Nanoencapsulated chitosan nanoparticles in emulsion-based oral delivery system: In vitro and in vivo evaluation of insulin loaded formulation. Journal of Drug Delivery Science and Technology. 2016;26:161-167. DOI: 10.1016/j.jddst.2016.10.010
  160. 160. Lucyszyn N, Ono L, Lumbambo A, Woehl M, Sens C, de Souza C, et al. Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan. Carbohydrate Polymers. 2016;151:889-898. DOI: 10.1016/j.carbpol.2016.06.027
  161. 161. Dispenza C, Todaro S, Bulone D, Sabatino M, Ghersi G, et al. Physico-chemical and mechanical characterization of in-situ forming xyloglucan gels incorporating a growth factor to promote cartilage reconstruction. Materials Science and Engineering: C. 2017;70:745-752. DOI: 10.1016/j.msec.2016.09.045
  162. 162. Mahajan S, Deshmukh R. Development and evaluation of gel-forming ocular film based on xyloglucan. Carbohydrate Polymers. 2015;122:243-247. DOI: 10.1016/j.carbpol.2015.01.018
  163. 163. Liu J, Li Y, Rong X, Lin W, Zhang T, Wang B, et al. Application of crystalline cellulose membrane (Veloderm®) on split-thickness skin graft donor sites in burn or reconstructive plastic surgery patients. Journal of Burn Care & Research. 2013;34(3):76-82. DOI: 10.1097/BCR.0b013e31825d5d8d
  164. 164. Mahajan S, Gundare S. Preparation, characterization and pulmonary pharmacokinetics of xyloglucan microspheres as dry powder inhalation. Carbohydrate Polymers. 2014;102(15):529-536. DOI: 10.1016/j.carbpol.2013.11.036

Written By

Diana M. Martínez-Ibarra, Jaime López-Cervantes, Dalia I. Sánchez- Machado and Ana Sanches-Silva

Submitted: October 4th, 2017 Reviewed: January 30th, 2018 Published: July 18th, 2018