Characteristics of the design areas and proposal.
\\n\\n
IntechOpen Book Series will also publish a program of research-driven Thematic Edited Volumes that focus on specific areas and allow for a more in-depth overview of a particular subject.
\\n\\nIntechOpen Book Series will be launching regularly to offer our authors and editors exciting opportunities to publish their research Open Access. We will begin by relaunching some of our existing Book Series in this innovative book format, and will expand in 2022 into rapidly growing research fields that are driving and advancing society.
\\n\\nLaunching 2021
\\n\\nArtificial Intelligence, ISSN 2633-1403
\\n\\nVeterinary Medicine and Science, ISSN 2632-0517
\\n\\nBiochemistry, ISSN 2632-0983
\\n\\nBiomedical Engineering, ISSN 2631-5343
\\n\\nInfectious Diseases, ISSN 2631-6188
\\n\\nPhysiology (Coming Soon)
\\n\\nDentistry (Coming Soon)
\\n\\nWe invite you to explore our IntechOpen Book Series, find the right publishing program for you and reach your desired audience in record time.
\\n\\nNote: Edited in October 2021
\\n"}]',published:!0,mainMedia:{caption:"",originalUrl:"/media/original/132"}},components:[{type:"htmlEditorComponent",content:'With the desire to make book publishing more relevant for the digital age and offer innovative Open Access publishing options, we are thrilled to announce the launch of our new publishing format: IntechOpen Book Series.
\n\nDesigned to cover fast-moving research fields in rapidly expanding areas, our Book Series feature a Topic structure allowing us to present the most relevant sub-disciplines. Book Series are headed by Series Editors, and a team of Topic Editors supported by international Editorial Board members. Topics are always open for submissions, with an Annual Volume published each calendar year.
\n\nAfter a robust peer-review process, accepted works are published quickly, thanks to Online First, ensuring research is made available to the scientific community without delay.
\n\nOur innovative Book Series format brings you:
\n\nIntechOpen Book Series will also publish a program of research-driven Thematic Edited Volumes that focus on specific areas and allow for a more in-depth overview of a particular subject.
\n\nIntechOpen Book Series will be launching regularly to offer our authors and editors exciting opportunities to publish their research Open Access. We will begin by relaunching some of our existing Book Series in this innovative book format, and will expand in 2022 into rapidly growing research fields that are driving and advancing society.
\n\nLaunching 2021
\n\nArtificial Intelligence, ISSN 2633-1403
\n\nVeterinary Medicine and Science, ISSN 2632-0517
\n\nBiochemistry, ISSN 2632-0983
\n\nBiomedical Engineering, ISSN 2631-5343
\n\nInfectious Diseases, ISSN 2631-6188
\n\nPhysiology (Coming Soon)
\n\nDentistry (Coming Soon)
\n\nWe invite you to explore our IntechOpen Book Series, find the right publishing program for you and reach your desired audience in record time.
\n\nNote: Edited in October 2021
\n'}],latestNews:[{slug:"webinar-introduction-to-open-science-wednesday-18-may-1-pm-cest-20220518",title:"Webinar: Introduction to Open Science | Wednesday 18 May, 1 PM CEST"},{slug:"step-in-the-right-direction-intechopen-launches-a-portfolio-of-open-science-journals-20220414",title:"Step in the Right Direction: IntechOpen Launches a Portfolio of Open Science Journals"},{slug:"let-s-meet-at-london-book-fair-5-7-april-2022-olympia-london-20220321",title:"Let’s meet at London Book Fair, 5-7 April 2022, Olympia London"},{slug:"50-books-published-as-part-of-intechopen-and-knowledge-unlatched-ku-collaboration-20220316",title:"50 Books published as part of IntechOpen and Knowledge Unlatched (KU) Collaboration"},{slug:"intechopen-joins-the-united-nations-sustainable-development-goals-publishers-compact-20221702",title:"IntechOpen joins the United Nations Sustainable Development Goals Publishers Compact"},{slug:"intechopen-signs-exclusive-representation-agreement-with-lsr-libros-servicios-y-representaciones-s-a-de-c-v-20211123",title:"IntechOpen Signs Exclusive Representation Agreement with LSR Libros Servicios y Representaciones S.A. de C.V"},{slug:"intechopen-expands-partnership-with-research4life-20211110",title:"IntechOpen Expands Partnership with Research4Life"},{slug:"introducing-intechopen-book-series-a-new-publishing-format-for-oa-books-20210915",title:"Introducing IntechOpen Book Series - A New Publishing Format for OA Books"}]},book:{item:{type:"book",id:"6191",leadTitle:null,fullTitle:"Selected Topics in Breastfeeding",title:"Selected Topics in Breastfeeding",subtitle:null,reviewType:"peer-reviewed",abstract:"Breastfeeding is a cornerstone of child nutrition and the growth and development of children. In addition, it generates other multiple benefits for both child and mother. Consequently, it has been recognized as a strategy of promotion and protection of the main health for different countries across the world. However, despite the strong evidence of its benefits and the public health policies being implemented to promote breastfeeding, the prevalence of exclusive breastfeeding at the sixth month does not reach the recommendations of many countries. This book intends to provide the reader with an overview of selected topics on current state-of-the-art breastfeeding in different situations and conditions. Specialists in the field of breastfeeding from different countries have developed these chapters and through them they share part of their experience.",isbn:"978-1-78984-909-7",printIsbn:"978-1-78984-908-0",pdfIsbn:"978-1-83881-325-3",doi:"10.5772/intechopen.68517",price:100,priceEur:109,priceUsd:129,slug:"selected-topics-in-breastfeeding",numberOfPages:78,isOpenForSubmission:!1,isInWos:null,isInBkci:!1,hash:"3334b831761ffa52e78de6fc681e33b3",bookSignature:"R. Mauricio Barría P.",publishedDate:"December 19th 2018",coverURL:"https://cdn.intechopen.com/books/images_new/6191.jpg",numberOfDownloads:6050,numberOfWosCitations:5,numberOfCrossrefCitations:3,numberOfCrossrefCitationsByBook:0,numberOfDimensionsCitations:8,numberOfDimensionsCitationsByBook:0,hasAltmetrics:1,numberOfTotalCitations:16,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"May 30th 2017",dateEndSecondStepPublish:"June 20th 2017",dateEndThirdStepPublish:"September 16th 2017",dateEndFourthStepPublish:"December 15th 2017",dateEndFifthStepPublish:"February 13th 2018",currentStepOfPublishingProcess:5,indexedIn:"1,2,3,4,5,6",editedByType:"Edited by",kuFlag:!1,featuredMarkup:null,editors:[{id:"88861",title:"Dr.",name:"R. Mauricio",middleName:null,surname:"Barría",slug:"r.-mauricio-barria",fullName:"R. Mauricio Barría",profilePictureURL:"https://mts.intechopen.com/storage/users/88861/images/system/88861.jpg",biography:"R. Mauricio Barría, DrPH, is a principal investigator and associate professor at the Faculty of Medicine, Universidad Austral de Chile. He was trained as an epidemiologist and received his MSc in Clinical Epidemiology from Universidad de la Frontera, Chile, and his DrPH from Universidad de Chile. His research interests include maternal-child health, neonatal care, and environmental health. He is skilled in epidemiological study design with a special interest in cohort studies and clinical trials. From 2010 until 2017 Dr. Barría was director of the Evidence-Based Health Office. He is currently the director of the Institute of Nursing, Faculty of Medicine, Universidad Austral de Chile.",institutionString:null,position:null,outsideEditionCount:null,totalCites:0,totalAuthoredChapters:"4",totalChapterViews:"0",totalEditedBooks:"5",institution:{name:"Austral University of Chile",institutionURL:null,country:{name:"Chile"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null,coeditorOne:null,coeditorTwo:null,coeditorThree:null,coeditorFour:null,coeditorFive:null,topics:[{id:"189",title:"Obstetrics and Gynecology",slug:"obstetrics-and-gynecology"}],chapters:[{id:"64417",title:"Introductory Chapter: A Comprehensive Approach to the Process of Breastfeeding",doi:"10.5772/intechopen.82177",slug:"introductory-chapter-a-comprehensive-approach-to-the-process-of-breastfeeding",totalDownloads:1288,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:null,signatures:"René Mauricio Barría P",downloadPdfUrl:"/chapter/pdf-download/64417",previewPdfUrl:"/chapter/pdf-preview/64417",authors:[{id:"88861",title:"Dr.",name:"R. Mauricio",surname:"Barría",slug:"r.-mauricio-barria",fullName:"R. Mauricio Barría"}],corrections:null},{id:"58668",title:"Bioactive Components of Human Milk: Similarities and Differences between Human Milk and Infant Formula",doi:"10.5772/intechopen.73074",slug:"bioactive-components-of-human-milk-similarities-and-differences-between-human-milk-and-infant-formul",totalDownloads:1740,totalCrossrefCites:3,totalDimensionsCites:5,hasAltmetrics:1,abstract:"Nowadays, there is an increasing awareness regarding the relationship between food, nutrition, and health. It is obvious that this relation starts from the birth. In the early stage of life, breastfeeding is considered the preferred choice for infant feeding and human milk is the optimal food for an infant to keep its nutritional and health status. Because it contains a large group of bioactive compounds such as proteins, vitamins, nucleotides, oligosaccharides, immunoglobulins, and some of the bioavailable minerals beyond its content of the essential nutrients, human milk is classified as the first functional food in the infant life. The various bioactive components of human milk play a pivotal role in preventing the gastrointestinal and respiratory infections, anemia, and bone-related problems as well as it enhances the immune function and helps in the maturation of the digestive system. The exclusive breastfeeding pattern during the first 6 months of infant life and introducing complementary foods after this period have a potential role in protecting against certain diseases in the adult stage of life. This chapter is underlying the great potential of breastfeeding for mothers and babies. Moreover, it discusses the functionality of some components of human milk and its similarities and differences between human milk and infant formulas.",signatures:"Esmat Aly, Aliaa Ali Darwish, Ruben Lopez-Nicolas, Carmen Frontela-Saseta and Gaspar Ros-Berruezo",downloadPdfUrl:"/chapter/pdf-download/58668",previewPdfUrl:"/chapter/pdf-preview/58668",authors:[null],corrections:null},{id:"58880",title:"The Influence of Breastfeeding and the Infant’s Social Environment on Neuroplasticity and Brain Development: The First 1000 Days",doi:"10.5772/intechopen.73209",slug:"the-influence-of-breastfeeding-and-the-infant-s-social-environment-on-neuroplasticity-and-brain-deve",totalDownloads:1061,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"There is substantial evidence that breastfeeding and an enriched environment provide significant contributions to the infant’s brain development. In the past 2 decades, there have been overwhelming data on the benefits of breastfeeding for 1 year and longer and its association with higher intelligence in later life. There is clear and convincing evidence from a number of disciplines, neuroscience, genetics, animal experiments and magnetic imaging techniques that indicate breastfeeding results in optimal brain development and higher IQ in later life. Magnetic imaging studies of infants, children and adolescents have provided significant evidence that the higher IQ in later life in breastfed infants is associated with larger brain size and higher degree of myelination of the white matter. Furthermore, observational studies of infants have provided clear evidence that breastfeeding and mother-baby sensory interaction result in significant cognitive and behavioral development of breastfed as compared to formula fed infants. Large-scale longitudinal studies of infants’ development have shown clear and convincing evidence of higher intelligence in children who were breastfed during infancy, and that the higher IQ persists through adulthood. In this communication, we provide evidence that breastfeeding and an enriched environment result in accelerated developmental potentials in the first 1000 days last a life time. The first 1000 days last the rest of our lives.",signatures:"Touraj Shafai, Monika Mustafa, Sandra Compsos and Lida Niake",downloadPdfUrl:"/chapter/pdf-download/58880",previewPdfUrl:"/chapter/pdf-preview/58880",authors:[{id:"192429",title:"M.D.",name:"Touraj",surname:"Shafai",slug:"touraj-shafai",fullName:"Touraj Shafai"}],corrections:null},{id:"63837",title:"Support for Breastfeeding",doi:"10.5772/intechopen.80383",slug:"support-for-breastfeeding",totalDownloads:920,totalCrossrefCites:0,totalDimensionsCites:0,hasAltmetrics:0,abstract:"Breastfeeding support for mothers of newborn babies in neonatal units is the basis for successful breastfeeding. With this, health professionals should educate the key members of the family and the environment surrounding the family about the benefits of breast milk in the first months of life and how to encourage and support the mother in the first months of life breastfeeding days. Exclusive breastfeeding is the most effective intervention to reduce infant morbidity and mortality and is estimated to prevent 13% of infant mortality under 5 years in low-income countries. However, the rate of exclusive breastfeeding is alarmingly low in developing countries. Mothers who face problems in breastfeeding immediately turn to high-quality milk formulas. Therefore, it is very important to assume the responsibility of health professionals to identify and adequately manage breastfeeding problems. UNICEF/World Health Organization, through the Baby Friendly Hospital Initiative (BFHI), has recommended good health care practices that support breastfeeding to increase the likelihood of optimal breastfeeding. The focus of breastfeeding in preterm infants and hospitalized term infants should must worry on the physical, emotional, legal, and social difficulties that may occur in the mother. It is necessary to consciously strengthen these terms for a successful breastfeeding.",signatures:"Patricia Triviño Vargas",downloadPdfUrl:"/chapter/pdf-download/63837",previewPdfUrl:"/chapter/pdf-preview/63837",authors:[null],corrections:null},{id:"58256",title:"Breastfeeding and Reduced Risk of Breast Cancer in Women: A Review of Scientific Evidence",doi:"10.5772/intechopen.72688",slug:"breastfeeding-and-reduced-risk-of-breast-cancer-in-women-a-review-of-scientific-evidence",totalDownloads:1041,totalCrossrefCites:0,totalDimensionsCites:3,hasAltmetrics:0,abstract:"Recent research shows that women who breastfed their children are at considerably less risk of developing breast cancer. Nonetheless, the results of other studies show that this greater protection only applies to pre-menopausal women. Based on the above results, there is still a certain controversy as to whether breastfeeding protects women against breast cancer. The main objective of this chapter is to provide a review of the scientific evidence regarding the relationship between breast cancer and certain aspects of pregnancy as breastfeeding period. For this purpose, it was conducted a systematic review in four databases (Web of Science, MEDLINE, Scopus and CINAHL), using the MeSH terms (Breast Feeding, Primary Prevention, Breast Neoplasms). The available scientific evidence justifies that breastfeeding for periods of over 6 months results in statistically significant reductions in the risk of developing breast cancer, the most common gynecological tumor in young women. However, it remains to be studied further whether the observed risk reduction applies to women with inherited susceptibility to develop breast cancer.",signatures:"Emilio González-Jiménez",downloadPdfUrl:"/chapter/pdf-download/58256",previewPdfUrl:"/chapter/pdf-preview/58256",authors:[{id:"77001",title:"Dr.",name:"Emilio",surname:"González-Jiménez",slug:"emilio-gonzalez-jimenez",fullName:"Emilio González-Jiménez"}],corrections:null}],productType:{id:"1",title:"Edited Volume",chapterContentType:"chapter",authoredCaption:"Edited by"},subseries:null,tags:null},relatedBooks:[{type:"book",id:"6550",title:"Cohort Studies in Health Sciences",subtitle:null,isOpenForSubmission:!1,hash:"01df5aba4fff1a84b37a2fdafa809660",slug:"cohort-studies-in-health-sciences",bookSignature:"R. Mauricio Barría",coverURL:"https://cdn.intechopen.com/books/images_new/6550.jpg",editedByType:"Edited by",editors:[{id:"88861",title:"Dr.",name:"R. Mauricio",surname:"Barría",slug:"r.-mauricio-barria",fullName:"R. 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In 2010, he received a Ph.D. in Egyptology from the University of Basel, Switzerland.\nFrom 2012 to 2017, Dr. Pereira was a post-doctoral fellow at CHAM/FCSH – Universidade Nova de Lisboa.\nIn 2018, he became an Onassis Fellow, hosted by the Department of Mediterranean Studies, University of the Aegean, Greece. \nIn 2019, he became an auxiliary researcher at CHAM/FCSH – Universidade Nova de Lisboa. 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Brain-like computation is about processing and interpreting data or directly putting forward and performing actions. Learning is a very important aspect. This book is on reinforcement learning which involves performing actions to achieve a goal.\r\nThe first 11 chapters of this book describe and extend the scope of reinforcement learning. The remaining 11 chapters show that there is already wide usage in numerous fields. Reinforcement learning can tackle control tasks that are too complex for traditional, hand-designed, non-learning controllers. As learning computers can deal with technical complexities, the tasks of human operators remain to specify goals on increasingly higher levels. This book shows that reinforcement learning is a very dynamic area in terms of theory and applications and it shall stimulate and encourage new research in this field.",isbn:null,printIsbn:"978-3-902613-14-1",pdfIsbn:"978-953-51-5821-9",doi:"10.5772/54",price:139,priceEur:155,priceUsd:179,slug:"reinforcement_learning",numberOfPages:434,isOpenForSubmission:!1,isSalesforceBook:!1,isNomenclature:!1,hash:"8a1290de1769ec93ed92327f93a9a4bb",bookSignature:"Cornelius Weber, Mark Elshaw and Norbert Michael Mayer",publishedDate:"January 1st 2008",coverURL:"https://cdn.intechopen.com/books/images_new/2220.jpg",keywords:null,numberOfDownloads:98560,numberOfWosCitations:80,numberOfCrossrefCitations:30,numberOfDimensionsCitations:75,numberOfTotalCitations:185,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:null,dateEndSecondStepPublish:null,dateEndThirdStepPublish:null,dateEndFourthStepPublish:null,dateEndFifthStepPublish:null,dateConfirmationOfParticipation:null,remainingDaysToSecondStep:null,secondStepPassed:null,areRegistrationsClosed:null,currentStepOfPublishingProcess:1,editedByType:"Edited by",kuFlag:!1,biosketch:null,coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"130979",title:"Prof.",name:"Cornelius",middleName:null,surname:"Weber",slug:"cornelius-weber",fullName:"Cornelius Weber",profilePictureURL:"https://mts.intechopen.com/storage/users/130979/images/system/130979.jpg",biography:"Cornelius Weber received the Diploma degree in physics, from the University of Bielefeld, Bielefeld, Germany, and the Ph.D. degree in computer science with the Technische Universität Berlin, Berlin, Germany, in 2000.He is a Laboratory Manager with the Knowledge Technology Group, University of Hamburg, Hamburg, Germany. 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There is a real threat of an environmental degradation. In 1968, the Club of Rome published a report where “limits of growth” were questioned [1] and highlighted the preoccupation with the existing economic models. In June 1972, the
The proposal of a sustainable development was consolidated and widely disseminated after the
According to Lindholm [6], urban planning shows a utopic aspect that has to be complemented with a practical “here and present” perspective. To do this, it is necessary to identify what kind of spatial relations are being created intending to discuss and allow new future possibilities.
Today, we already fully understand that an urban planning cannot be separated from the environmental planning. Cities managed without integrated planning suffer with the loss of ecosystems and of its environmental and cultural values. Inundations, pollution, scarcity of water, shortcomings in public health, among many other grave problems, arise from this lack of integration.
Without intending to create an only definition for a so wide and complex topic, it is here understood that a sustainable city is the one which meets the social functions that are expected from it, through time, in a viable way and with a general positive balance, reaching an adequate quality of life for all its inhabitants, guaranteeing the distributed access to essential services and recourses, respecting the limits imposed by the natural system, being resilient and secure.
The definition of resilience [7, 8, 9, 10, 11, 12, 13, 14, 15] is a concept very much present in urban discussion nowadays [16, 17]. The resilience involves, generally (and with various interpretations, depending on the topic of the discussion), three main points: the capacity of a system to continue resisting, even if submitted to stressing conditions beyond those projected; the capacity of the system to recuperate functions in a fast way, continuing to offer its services; and the capacity of the system to recuperate structurally from suffered damages [18]. Meerow et al. [19] stated that resilience is a dynamic concept, recognising the importance of its temporal scale, where a systemic adaptability applies. This perception considers the urban system as a complex and adaptable system composed by socioecological and sociotechnical patterns that go through multiple spatial scales. In this way, the concept of resilience approaches the discussion about sustainability [20, 21, 22, 23] as both concepts involve an integration through time, in which the systems must continue to function and provide adequate services today, as well as for future generations, submitted to future challenges.
McHarg [24] points to the necessity of considering landscaping as an ecological system, where the prime concern refers to the integration of social and environmental issues within the planning process. This author proposes a new perspective for a healthy relation between nature and the built environment, understanding the processes that shape landscape and using them as the basis for an efficient planning process. Rogers [25] poses that at the same time, the cities reunite and potentiate physical, intellectual and creative energies, they tend to damage natural environment configuring a threat to the very humanity itself. Today, cities are better known by their conflicts and problems than their potentialities. Harvey [26] highlights that the right to do (and redo) the cities (and ourselves), evolving towards a better place, is one of our most precious and neglected rights.
Various discussions are being motivated by the necessity of making the cities more sustainable [27, 28, 29]. Among these discussions, one that has been gaining space refers to the compact and diverse city concept [30, 31, 32, 33, 34, 35], in which the networks of the infrastructure become less extensive and more efficient, less land is consumed and the reduced commuting consumes less energy. Burgess [36] defines the compact city as the one that results from a set of politics seeking to increase the constructed area and the demographic densities, to intensify the economic, social and cultural urban activities and manipulate the size, the form and the urban structure as well as the housing systems. This concept benefits from the environmental, social and global sustainability that derives from the concentration of urban activities. It is further believed that in more compact and diverse cities, social relations are intensified, increasing the process of democratisation. For Rogers [37], a city is sustainable when it is fair, pretty, creative, ecological, easy, compact, polycentric and diversified. Nevertheless, if extremely densified, cities that are more compact may lead to greater local negative ecological impacts, when compared with that resulting from less compact cities. There certainly is a limit for compaction, equalising the supporting capacity of the natural environment and the optimisation of the built environment. In this way, a balance shall be established between the built and the open spaces, defining the degree of city compaction affecting its sustainability. The idea is not creating an absolute rule, as all cities are cultural representations of huge magnitudes. However, a point of equilibrium for each city in its diversity must be found.
In this discussion about balancing built and open spaces, urban flooding arise as an element of disruption of urban services, of infrastructure networks, of housing and health systems. When urban rivers and drainage networks fail, leading to flooding, they produce urban negative consequences and, very often, irreparable socioenvironmental damages. However, although extensive flood control structures have been implemented in the recent years (e.g. dikes, dams, canals, pump stations, etc.), cities everywhere remain vulnerable to floods [38]. Ecologically, more sensitive mitigation approaches have appeared in recent years, encouraging flood hazard mitigation to work with nature [39]. Grizzetti et al. [40] remember that a territorial planning for urban water management is needed, and system multifunctionality has to be recognised as well as the benefits of approaching city and nature. Conceptually, urban drainage is an infrastructure system that occupies a key position interfacing natural demands (rainfall-runoff transformation and consequent discharges) and city needs (healthy neighbourhoods and city functions maintenance).
The capacity of the environment to support a human settlement can be defined as the maximum elasticity to which the environment can be subjected, without any irreversible degradation, to attend the urban necessities that will be developed systematically in this space.
The process of urbanisation, generally, demands multiple and continued efforts to satisfy the necessities of the population. However, this process, if not planned or barely conducted, causes harmful effects on the environment, resulting in a possible degradation of the natural environment as well as of the built environment. As the urban population increases, various problems related with the necessary infrastructure to attend this growing demand also increase. Many times the concept of supporting capacity is reduced to the capacity of the infrastructure to continue meeting the demands of the city development. However, from a systematic point of view, one must not overlook the wider concept of the supporting capacity and the perception that the built environment needs to respect the limits imposed by the natural environment.
As introduced earlier, the urban drainage system occupies a very specific position in this discussion. This system lies between the demands of the natural environment and the necessities of the built environment: the rain that falls on a watershed and needs to flow through the city is able to find its way within the drainage system, which offers, in turn, secure conditions for the city to occupy the natural space [41]. Therefore, the city may coexist with the natural watershed in a healthy combination [42]. Nevertheless, the very urbanisation modifies the natural space and the way in which this space responds to the water cycle, aggravating floods [43, 44]. In this way, environmental issues specifically linked to the urban drainage becomes more evident, once failures of the drainage system affect the functioning of the city, reflecting in vectors of environmental degradation. These are key questions for urban planners and decision makers, since they are structuring factors for planning the space and its sustainable occupation.
However, it is frequent that cities exceed the supporting capacity, consuming more than necessary (depleting natural recourses and expanding over the watershed space, modifying land use) and creating more and more waste (which the environment cannot absorb). The areas of a city which usually are exposed to more extreme conditions, with high densities of population, occupation and construction, with fewer open spaces and higher inundation risks, are also usually related with the more critical shortcomings of infrastructure and higher economic and social losses.
When modifying nature, without considering the supporting capacity of the environment, by means of unrestrictedly building and spreading urban growth, the population suffers with the reduction of environmental quality, which could directly and/or indirectly interfere in the quality of people’s lives. Some consequences of this development, such as vegetal cover removal, erosion and/or silting-up of watercourses, lacking of open spaces, air and water pollution, inadequate deposition of garbage and sewage, among others, bear witness to the disequilibrium between the cities growth and the functional aspects of the natural environment.
Several studies have been realised and a series of efforts have been made to establish indicators for sustainable development. In 1996, for example, the Commission of the United Nations for Sustainable Development announced a project to evaluate and compare the degree of sustainable development for each country, generating indicators [45]. Since then, several indicators have been developed to evaluate the results of urban growth. Such indicators generally include social, economic, environmental and institutional categories. Among those, the “environmental” dimension ought to be the prime preoccupation in the pursuit of an environmentally healthy and sustainable development.
Kyushik et al. [46] discussed the urban carrying capacity as a determinant for the developing density and presented some authors who also discussed the supporting capacity concept. In this discussion, they firstly cite Chung [47], who stated that the ecologists generally consider the supporting capacity as the maximum number of individuals who can be supported by the environment in a given area, without compromising future generations living in this area. Planners generally define the supporting capacity as the capacity of a natural or artificial system to absorb the growth of the population or the physical development without considerable degradation or damage [48]. It is also defined as the level of human activities, growth of the population, land use and physical development, which could be sustained by the urban environment without causing serious degradation and irreversible damage [49]. This concept is based on the assumption that a certain environmental thresholds exists, which, when crossed, can cause serious and irreversible damages to the natural environment [50].
Considering the discussion developed and the definitions cited above, one can conclude that the limit of the supporting capacity of the environment is interlinked with the social environmental quality of the urban space. For this reason, the urban planning process and the discussion about socioeconomic, morphologic and functional aspects of the city have to incorporate the supporting capacity concept.
The urban planning integrated with the environmental planning (or, simply, the urban environment planning), can provide an improvement in the quality of life in cities. However, this is only possible when some concepts and relations are understood linking environment, open spaces system and cultural landscapes.
Tuan [51] stated that “environment sustains us as creatures; landscapes display us as cultures”. Schlee et al. [52] stressed that “the landscape is a product deeply impregnated with culture, which results in processes of continuous alterations, dictated by biophysical, social, economic and political factors, reflected in the forms of occupation and management of the territory”.
Therefore, a landscape, and especially the urban landscape, is not only a cultural representation of a society but also a set of human actions in an environment which establishes itself in an integrated way, but not without conflicts during time. For Meining [53] “any landscape is so dense with evidence and so complex and cryptic that we can never be assured that we have read it all or read it right”.
In this way, every urban landscape is distinct, but all of them consist of built spaces and non-built spaces—these last ones are the open spaces free of construction, either being public or private. Macedo and Queiroga [54] understand the open space system as “all the elements and the relationships that organise and structure the set of all open spaces of a determined urban area – from intra-urban to regional scale. It is basic in the existence of the city, because it is fundamental in the performance of the day to day life and in defining the urban form and the image of the city, reflecting its history and memory, participating in the public and private life spheres”.
Rego et al. [55] define the open urban spaces system in: (1) environmental open spaces; (2) social interaction open spaces, such as parks, squares or gardens; (3) open spaces of infrastructure character, such as a strip of land protecting transmission lines; and (4) open spaces of mobility character, such as streets, roads and avenues.
Ironically, every city starts to be designed by its streets. These are the ways which connect the various parts and structured elements, leaving the first marks on the land which will define a human settlement. Therefore, the occupation/construction of a city is initiated by an element that afterwards will be named as an open space, which defines not only the border between what is public and private, but also where the largest part of the urban network infrastructure will be implemented.
It has to be pointed out that, at times, the open urban spaces are only apparently free of construction as the substratum is occupied by large structures such as metro, underground roads, garages or even canalised rivers.
In this way, it is understood that the open spaces system of a great number of consolidated cities is not the result of an environmental urban planning but the result of a process of hundreds or thousands of years which today, many times, is insufficient or inadequate to couple with the socioenvironmental demands.
Generally, the segmented organisation of a territory, without the proper preoccupation regarding its recourses, especially referring to open spaces, is one of the facts that characterises the urban sprawl of current metropolis. Urbanisation spreads without borders and without giving attention to the collective necessities, favouring a general disequilibrium [56]. A systematic and integrated vision is necessary, associating environmental questions and urban infrastructures. Public politics should be concerned in re-evaluating land use and the occupation, seeking to adopt more resilient and sustainable urban standards. The open spaces always present a great possibility to suffer and generate transformations (positives or negatives) in the landscape shaping (or re-shaping). Urban spaces are simultaneously the most promising and most fragile spaces, because they are subjected to inadequate or disorderly occupation, when their fundamental importance is not recognised [24].
This fact occurs in many cities in developing countries, from small town to metropolis, where open spaces, generally of environmental character, are ending up being occupied in an irregular manner by substandard dwellings of a population economically and socially less favoured, such as on hill sides, mangroves areas, the margins of rivers and lakes, following an accelerated population growth which is neither accompanied by a socioeconomic development, nor by an adequate infrastructure support.
A strategy to restrain the irregular occupation of those open spaces is to have them recognised as public structures with a social and patrimonial value, that is, spaces for congregating social groups, while appropriate for collective leisure.
Chiesura [57] argues that urban parks and open green spaces are of strategic importance for the quality of life of our increasingly urbanised society. Increasing evidences indicate that the presence of natural assets (i.e. urban parks and forests, green belts) and components (i.e. trees, water) in urban territories contributes to the quality of life in many ways. Besides, important environmental services such as air and water purification, wind and noise filtering, flood control or microclimate stabilisation, natural areas provide social and psychological services, which are of crucial significance for the liveability of modern cities and the well-being of urban dwellers.
Nevertheless, the lack of open spaces in consolidated cities is frequent, as it is frequent that such spaces are not adequately foreseen in still growing cities. To minimise the conflicts in the demand for space, the concept of multifunctional spaces could be the answer to optimise the landscape design, associating the open spaces with the urban occupation demands, without losing any functions already existing today.
A multifunctional space is an urban intervention which foresees more than just one use for the same place, guaranteeing a more rational use of the urban land. Different uses can target infrastructure aspects, such as urban drainage, as well as trying to overcome social privation, such as the absence of leisure areas.
It is necessary to densify cities into more compact arrangements, associating diverse and multiple uses to urban spaces, intending to optimise commuting and the several services offered, without eliminating open spaces. These spaces need to be preserved as a basic support for a city capable of meeting the environment, infrastructural and social needs, corroborating with the very concept of sustainability, also emphasising the well-being and the quality of life of the population.
In this way, the solutions for urban drainage problems should be able to integrate the response to urban demands regarding sanitation purposes with the supporting capacity of the urban watershed, using multifunctional spaces to reorganise the water cycle functions that were modified by urbanisation. Spirn [58] criticises superficial urban projects, created only to beautify the city with an artificial nature. For the author, radical changes are necessary, seeking to recognise and point out a series of damages, mainly in artificialized urban rivers, which do not respect the necessities of nature and do not consider the river as an essential strength that permeate the city. According to Naveh [59], one of the premises for a holistic concept of multifunctional landscapes is that these represent a complex interaction between nature and culture.
In this context, all the aggregated values of landscapes should be encouraged: their environmental aspects; their cultural, social and economical values; defending the environmental legislation as part of the regulation for a proper urban expansion, with the purpose of bringing the concept of supporting capacity to the discussion of urban and environmental planning.
Water is probably the most essential natural resource and relates to diverse aspects of human settlements, from the beginning of the history, ranging from water supply, irrigation, transportation, territory defence, among others. From ancient times, man always looked for locations where the environment could give support for his survival. Great part of the first civilisations developed along river banks or near lakes.
Any city, however, tends to introduce modifications in the land use patterns, which induce a series of processes that change the environment, affecting the quality both of the natural and the constructed areas, and many of the supervening consequences are related to urban waters, in its wider sense.
Therefore, a paradox exists in the relationship between water and city [41]. Water is a fundamental resource, which is present in the history and in the origin of cities, but is also a problem in modern cities. Actually, for this reason, it is not unusual that cities turn their back to the rivers, which are degraded, polluted, frequently seen as sewage conveyors or buried and hidden. Most urban rivers are lost as landscape elements, impoverishing the urban biodiversity and degrading also the urban vicinity [41, 42].
The rivers, in general, can be considered as a synthesis of the territory to which they are connected [60]. Or rather, the actions which take place in the watershed reflect in the fluvial corridor. In this way, it can be said that artificial rivers, degraded, suffering from flooding and degrading the city, end up to be the product of the urban environment itself, which have dis-characterised the natural processes of their hydrographic basin.
The urbanisation process strongly alters the natural water cycle and the responses of the fluvial systems on the built environment. The removal of vegetation, the increasing imperviousness that follows this process, the regularisation of the surfaces and the introduction of an artificial drainage system modify significantly the superficial flow patterns, producing larger flow volumes and peak flows, reducing the base flow discharges and the time of the concentration of the basin. A frequent result of this process is the aggravation of flood problems observed in cities and the loss of fluvial ecosystems.
Urban flooding is strongly related with land use questions. Typical urban factors, such as housing shortage, for example, turn up as aggravating agents. The irregular occupation of riverine areas, which should be used as river space, increases flooded areas and flooding consequences. The floods, in turn, are responsible for the degradation of the urban built environment, interfering with various other urban systems. They generate damages to structures and urban equipment, devaluate areas subjected to inundation, induce losses associated to paralysed businesses and services, interrupt the circulation of pedestrians and transport systems. They spread waterborne diseases, affecting and being affected by the improper collection and disposal of urban sewage and solid waste. The system weakens as a whole, risks increase and a degradation cycle emerges [41]. In resume, the impacts of urbanisation are presented in Figure 1.
Impacts of urbanisation on inundations [
The main gaps of integration between drainage design and urbanisation can be cited in a resumed way, as given in the following:
When the increasing urban growth is not controlled, the exceeding soil sealing by impervious surfaces generate superficial flows surpassing the capacity of the drainage network, going beyond those discharges foreseen in the project horizon;
lack of urban growth control in the total project area (mainly upstream), advancing towards areas which were originally natural, also increases discharges in the drainage network;
unsuitable occupation of the bottom of valleys and riverine areas, which should be preserved as flood plains, directly exposes the communities installed there;
lack of integration between drainage systems and the other basic sanitation systems, which need to be understood as complementary and not independent systems.
A series of reciprocal interferences can be mapped between the drainage system and the other urban systems, producing faults in cascades, as in a house of cards.
Gusmaroli et al. [61] proposed the adoption of an ecosystem approach to face river problems in urban areas, with the objective to amplify the concept of Waterfront Design, in which one seeks to improve the value of the contact line between city and water surface, reintroducing the watercourse in the city landscape. This approach widens the possibility of just using the presence of water as an aesthetic urban value, but also, and mainly, as an environmental asset, an element for connecting the city with nature. This possibility bring an opportunity to pursue the concept of fluvial restoration, from the point of view of an effective environmental improvement, looking at the city as a live organism in constant transformation and, due to this characteristic, capable to remodel and adapt itself (even if only partially, given all the modifications already suffered) to the demands of a more natural watercourse. The perspective to incorporate concepts of environmental sustainability into the process of rethinking the city growth opens a diversified set of opportunities to be explored as integrated solutions in a multidisciplinary context.
The traditional practices associated with drainage projects tend to focus on the problem of the resulting discharges in the storm drains and canals, equating them to these discharges. In this concept, the water needs to be rapidly conducted out of the watershed. This concept is very often responsible for transferring of flooding problems to downstream reaches situated in the lower river basin, without really solving the drainage problems.
Based on these questions, along the last decades, this traditional concept is being complemented or replaced by concepts which seek systematic solutions for the basin, with distributed interventions, looking to recover flow patterns similar to those existing before urbanisation. The new approach joins preoccupation of sustainable management of the urban rain waters, integrating those with urban space solutions. Measures for storing waters and incrementing the infiltration appear to be the alternative to treat the principal modifications introduced by the city growth on the water cycle [41]. These are called compensatory techniques [62], because they seek exactly to “compensate” the effects of urbanisation on the water cycle.
A resumed history of the conceptual evolution of urban drainage solutions since the Industrial Revolution is presented as follows:
The necessity of urgent sanitation appeared as a primary concern in industrial cities. As they suffered from serious problems of epidemics, this fact led to the development of hygienist concepts in drainage projects, reflecting on the necessity to convey as fast as possible rain waters and sewage—storm drains and artificial canals were adopted as frequent solutions and the fluvial and sanitary systems worked together.
The possibility of environmental degradation, the great rainfall intensities and the difficulty to treat diluted sewage led to the concept of separate sewer network at the beginning of the twentieth century.
Until approximately the 1970s, the drainage focus was set on the increase of conveyance and the necessity to improve flow conditions to face the increase of run off generated by the cities growth.
Since 1970, the traditional paradigms started to be broken.
The vision that the traditional measures of canalisation tended to transfer problems became dominant.
Compensatory techniques were adopted to control the distributed generated run off.
On source controls to minimise run off were introduced.
Quantity and quality of urban waters became integrated concerns.
The concept of “sustainable drainage” was formalised, seeking to: minimise the transfer of flood problems in space (for other locations) and in time (for future generations); integrate drainage solutions with socioenvironmental aspects; increase the biodiversity in the built environment; revitalise the urban spaces and articulate with urban development plans.
Rivers came to be seen as possible drivers for restructuring the urban landscape.
Rain water started to be seen as a usable resource.
In this new approach, the objectives of an urban drainage system, in a wider context, can be defined as:
The reduction of inundation of a given region of interest and the minimisation of damages to the community installed in the drained basin.
Integration with the urban developing plan, both in what regards land use questions and the future urban growth.
Preservation of riverine areas, as well as the integration of drainage solutions within urban landscapes, in multifunctional combinations.
Integrated evaluation of questions regarding quality and quantity of the urban waters.
A compromise between the drainage of the interest region and final destination in the receiving water body, without transferring problems downstream.
It is necessary to evaluate the points of conflict between the natural and the built environment, searching for means of convergence, equalising conflicts and potentialising synergies and positive results.
The search for sustainability need to be kept in the conjugation of the social, economic and environmental scales, with lasting results, as sustainability is a concept that directs the vision to the future. Therefore, a solution that intends to be sustainable needs to be able to stand and face future challenges. This characteristic converges to the concept of resilience. In this context, the sustainable development is capable to face future risks supported by the environmental resilience.
A set of premises is suggested here to direct the major drainage projects towards an effective solution for the urban flooding, considering an integrated vision for the river and the city, with a complementary (and not a competitive) approach regarding the natural and built environments. In this sense, the creation of “multifunctional spaces” allows that the open spaces remain in the urban cores already consolidated, associating hydrologic functions to the already existing socioenvironmental and economic functions.
The utilisation of these open spaces can permit:
to control and reorganise flow discharges avoiding flooding;
the introduction of fluvial parks, which may be used for leisure, landscaping and flood storage, besides helping the organisation of urban space and creating limits for the urban growth (preventing the city to advance over preservation areas);
the increment of urban biodiversity;
the use of green ways with the purpose of mobility and connection of the fragmented environmental areas;
the valorisation of property in the neighbourhood;
the eventual waterway transportation in specific cases.
Therefore, the creation of multifunctional spaces can (and should) be linked to a strategy that embodies the concepts of fluvial restoration. Such an action foresees the environmental recovery of the fluvial corridor, as an additional component of the sustainable urban drainage approach, which, in turn, offers a distributed action in the watershed, reorganising flow patterns and restoring water cycle functions, preventing flooding.
Dona Eugênia River Basin (coordinates 22° 46′ 55″ S, 43° 25′ 44″ O) drains a watershed of 18 km2 situated in the Rio de Janeiro metropolitan area, crossing two cities of a lowland region called Baixada Fluminense: Nova Iguaçu and Mesquita. Dona Eugênia River is about 10 km long. The first 4 km are located in Nova Iguaçu inside an environmental preservation area called Gericinó/Mendanha. The subsequent 6 km run through the city of Mesquita, crossing a dense urban area until its outfall at Sarapuí River. The preserved upstream area contrasts with the degradation found in the lower urban area, just a few kilometres downstream, and is a reference for restoration goals. The climate is hot and humid with a summer rainy season (Aw in the Koppen climate classification). It has an average annual temperature of 22°C and an average annual rainfall of 1700 mm [63]. Mesquita has approximately 170,000 inhabitants, according to the 2010 Brazilian Census [64] and the Human Development Index calculated for the city and reported by the United Nations Development Programme in 2010, is 0.737, which places Mesquita at the 850th on the list of Brazilian municipalities.
The city has many infrastructure problems. According to Ref. [42], in the most populated areas of the city, there are numerous shantytowns built illegally, without considering environmental risk factors, both in flat flood prone areas and on steep hillsides. Even in areas regularly occupied, urban growth was not controlled, urban occupation sprawled, open areas are few and infrastructure was not adequately provided. The main problems of this city can be summarised as follows:
Intense and irregular informal settlements on riverine areas;
Lack of vegetation in the urban reaches of the river;
Urban settlements in risk areas, including dwellings on river banks, where the houses act as dikes, “canalising” the river;
Parts of the main river run canalised underground;
Recurrent problem of flooding, which affects about 80% of the city population;
Sedimentation at various points of the river;
Discharge of sewage and solid waste into the river, with visible environmental degradation and health hazards;
Scarce infrastructure;
Degradation of the urban environment.
In the recent past, the municipality has canalised Dona Eugênia River towards its upstream reaches in an artificialisation process that is stressing the environmental protection area, while configuring a loss of opportunity to integrate the river as an environmental asset in the urban landscape. The consequences are clear: less biodiversity, fewer open green areas, less leisure opportunities, greater environmental fragmentation (with consequent degradation) and worse floods downstream.
In the context of the discussion developed in the previous sections of this chapter, this case has motivated a research joining the necessity to find a flood control alternative, the aspiration to revitalise the degraded urban areas and the goal to improve environmental conditions. The main part of this research is concerned with the reorganisation of the open spaces system, providing a storage capacity to the urban drainage system, while using the river path as a corridor to integrate environment fragmented areas among them and with the upstream protected forest.
A mathematical hydrodynamic model—MODCEL—developed in the Federal University of Rio de Janeiro (UFRJ) was used to produce flood maps and then evaluate flood conditions. Due to the limited availability of data, the model calibration used the results of a previous study as reference. This study was, carried out by the Rio de Janeiro state government, in the revision of the
The diagnosis of the current situation considered as a design storm event with a 25-year return period, as recommended by the Ministry of the Cities, in Brazil. Figure 2 shows the flood map obtained for this situation, confirming the criticality of the flooding problem in Mesquita. The mathematical modelling showed areas with inundation levels reaching more than 0.75 m in some places. The critical points are associated with the city centre region and in riparian areas with informal settlements. This antagonistic situation shows that both formal and informal urban areas suffer from significant flooding. The region located upstream the railway line is also critical, once the railway walls act as a barrier to runoff and urban storm drains are insufficient.
Current situation—flood map—recurrence period 25 years (Source: see Ref. [
It is important to note that some flooding observed in the current situation is due to the lack of minor drainage in the basin. Thus, floods are retained in the plains and cannot reach the river rapidly. The city centre is greatly affected: both major and the minor drainage system fail. The plains near the river, at the city centre and a natural wetland downstream (where some informal settlements appear) show the highest flooding levels.
An interesting observation related to a past situation helps to illustrate the discussion. In the beginning of 1970 decade, only the area upstream the railway was urbanised (Figure 3).
Past situation (1970) (Source: see Ref. [
Mesquita population, according to IBGE census for 1970 [65], was 93,678. Considering that soil imperviousness is proportional to the population density, it was possible to simulate floods in 1970. Taking the same rainfall event of 25 years of recurrence period, flood maps simulated for 1970 were compared with the ones obtained for 2010 (Figure 4). Two main observations arise from comparing these maps:
In the downstream areas, where no urbanisation took place, there were no flooding problems.
In the upstream area, although with fewer people and less imperviousness, floods were already significant (even if less important than today). This means that the space that should be occupied by flood flows was inadvertently occupied by the built environment.
Current and past (1970) situation—flood maps—recurrence period 25 years (Source: see Ref. [
Flood control is a matter of allocating spaces. Flood flows need space and the open spaces system of a city can be used to fulfil this aim.
After completing the flooding diagnosis, an alternative has been studied to solve this problem, with the expectation of avoiding river overflows and organising major drainage demands, using the open space system to reorganise flow patterns, but also providing river improvement and urban revitalisation, increasing both natural and built environment values. This alternative combined River Restoration (RR) solutions and Sustainable Drainage (SD) techniques, acting through the spaces provided by the open spaces system. In this alternative (RR + SD), the river was analysed and divided in three stretches in the urban area, identified as different landscape units: an upper reach near the natural protected area (area 1—upstream); a central area in the core of the city (area 2—middle reach); and a wetland in the river outfall (area 3—downstream), as schematically shown in Figure 5.
RR + SD alternative (Source: see Ref. [
All the three areas suffer from informal settlements, substandard habitations and flood risks. Taking into account that it is a consolidated urban watershed, the open spaces shall be considered with multifunctional characteristics (landscaping, recreation, biodiversity and flood control, for example).
The design project proposals are listed in Table 1 and Figures 6–9 show the concepts supporting the proposals. The total storage volume offered by the three areas, in the combined RR + SD alternative, sums approximately 270,000 m3, with additional contribution of infiltration measures and on-site detentions (adding 106,000 m3).
Watershed Reaches | Characteristics | Vocation | Proposal |
---|---|---|---|
Area 1 (upstream) | This area is the most upstream urban reach. Urban growth is spreading and forcing the occupation of the valley that leads to environmental protection area. Substandard houses, poverty and an informal growth mark this area. Natural and built environment degradation is spreading. Floods are not observed in this area | This area may act as a transition from the city to the upstream protected areas, working as the entrance door linking the city with the upper green park. It is possible to use the hills and the steeper slopes to propose a dam for temporary flood storage purposes. |
|
Area 2 (middle reach) | This area is the most floodable one. It is also the densest occupied area. The Major hall is here, in the city centre, near the river. There are also a line of houses, stacked on both riverbanks, strangling the river, discharging their sewage and hiding the river from urban landscape. | This area should provide space for flood storage. However, multifunctionality here is a key concept, once free space is rare. |
|
Area 3 (downstream) | This is the lowest area, near the outfall on the Sarapuí River. It is a floodable area, and there are slums spreading fast. It is also a swampy area. | This area can be used as a humid park, also contributing in improving water quality. Urban occupation should be avoided |
|
Characteristics of the design areas and proposal.
RR + SD alternative – area 1 (upstream) proposals (Source: see Ref. [
RR + SD alternative – area 2 (middle reach) proposals (Source: see Ref. [
RR + SD alternative – area 2 (middle reach) – details (Source: see Ref. [
RR + SD alternative – area 3 (downstream) proposals (Source: see Ref. [
Figure 10 shows the flood map for the alternative proposed. It is noticeable that flooding levels reduce all over the basin. The modelling system did not represent minor drainage, what is probably the explanation to the remaining flooding areas. These water depths are low and the river does not overflow anymore. Additionally, the city of Mesquita currently marked by a grey landscape has the opportunity to revitalise degraded urban areas, also improving environmental connections.
RR + SD alternative – flood map – recurrence period 25 years (Source: see Ref. [
The urban flooding problem can be seen as problem of space allocation. In using areas that should be preserved for inundation purposes and encouraging a land use that generates a major quantity of runoff, the process of urbanisation must foresee new and alternative spaces to temporarily store floods. In this way, the drainage system may continue working, and, consequently, the whole city may preserve its functions.
Therefore, one is aware of the necessity of utilising open spaces as fundamental pieces for mitigating floods in the cities. By doing this, the limited discharge capacity of the traditional drainage system, whose result is converted into a flooding volume spread throughout the city, can be resolved when the open spaces are considered as a possible reservoir to store exceeding volumes of the drainage system. In this approach, open spaces act as multifunctional landscapes. In this sense, the creation of multifunctional spaces can permit that the remaining open spaces in the already consolidated urban centres incorporate hydrologic functions to their original purposes.
It needs to be pointed out, nevertheless, that the city growth, in an uncontrolled form, leads to urban sprawl, on the one side, and the discussion of sustainability considering the concept of compact cities, on the other side, can both lead to a suppression of open spaces. Therefore, the urban and environmental planning, taking into consideration the supporting capacity of the natural environment in which the city is inserted, make it possible to improve the quality of life in cities.
The set of actions in the urban tissue, utilising open spaces to support drainage solutions, acting in the fluvial corridor and combining efforts to revitalise the urban environment, poses a sustainable approach for facing urban floods within an agglomeration trend.
The case study chosen to illustrate this discussion, and shown in the previous section, confirms the proposed concepts, collaborating for an urban sustainable development, through a model of interventions capable to embrace environmental, economic and social aspects.
Food matrices having water activities above 0.9 and wet food processing environments are wonderlands for microorganism multiplication and biofilm development. Biofilms are considered of great concern in regard to functioning of mechanical parts that may be blocked, to energy consumption, which becomes higher when heat transfer decreases, and to corrosion as corrosion rate of surfaces increases underneath biofilms (corrosion grows 10–1000 times faster causing loss of material and increasing porosity) but their presence in food and food processing environments is also a serious public health risk due to problems associated with foodborne illnesses and food spoilage [1].
\nThe biofilms that are threatening the safety of food products are produced by some pathogenic bacteria such as
Biofilms are formed on all types of surfaces existing in food plants ranging from plastic, glass, metal, cement, to wood and food products [4]. Usually, biofilms form a monolayer or more often multilayers, in which bacteria may undergo a significant change in physiology with an increased tolerance to environmental stresses [5].
\n\n
\n
Flagella, pili, and membrane proteins are also used by
In general terms, different
\n
\n
\n
Within the biofilm,
Four mechanisms based on the flagellar motility of
It has been showed that, in its planktonic form,
Over time, beside the conditions that favor the biofilm formation in food processing plants, the genetic background of biofilm forming microorganisms was also intensively studied. At each step of biofilm development and dispersal, there is a specific genetic signal control.
\nThe
In the biofilm formation, the attachment step is a prerequisite in which flagella and type I pili-mediated motilities are critical for the initial interaction between the cells and surface.
\nIn order to find out the roles of the genes and regulatory pathway controlling the biofilm formation, researchers applied one or two genome-wide approaches, like transposon insertion mutagenesis or/and transcriptome analyses. With a transposon mutagenesis library, it was possible to identify 70
Gene/KEGG/protein encoded | \nGene function | \nRole | \nBacterium | \nRef. | \n
---|---|---|---|---|
\n | \nMotility Flagella bio-synthesis | \nCell adhesion and bacterial attachment | \n\n | \n[23–25] | \n
\n | \n||||
\n | \nEnable the polymerization of the flagellin monomers; flagellar capping protein | \n[148] | \n||
\n | \nFlagellar motor rotation | \n|||
\n | \nDNA-binding transcription factor activity | \nPositive regulation of single species biofilm formation | \n\n | \n|
\n | \nEnable bacteria to colonize the host epithelium | \nCell adhesion | \n\n | \n[30, 31] | \n
\n | \nMotility bacterial-type flagellum assembly | \n|||
\n | \nFimbrial bio-synthesis | \n|||
\n | \nRepresses fimbria genes | \nSingle-species biofilm | \n||
\n | \nAcetylglucosaminyl transferase activity, cell adhesion | \nInvolved in the polymerization of a biofilm adhesin polysaccharide | \n\n | \n[149] | \n
\n | \nInvolved in gluconeogenesis pathway | \nRole in adherence | \n[150] | \n|
\n | \nMediates binding to human platelets | \nPlays a positive role in biofilm formation | \n[151] | \n|
\n | \nRegulatory role in sporulation | \nSingle-species surface biofilm formation | \n\n | \n[152] [153] | \n
\n | \nTransition to growth phase; flagellum formation | \nBiofilm formation | \n[154, 155] | \n|
\n | \nControls the rotational direction of flagella | \nMotility, cell adhesion | \n\n | \n[156] | \n
\n | \nPromotes the attachment to the surface | \n[157] | \n||
\n | ||||
\n | \nCatalyzes the first step in the D-alanylation of lipoteichoic acid (LTA) | \nCell wall biogenesis | \n\n | \n[24] | \n
\n | \nCarrier protein involved in the D-alanylation of LTA | \n|||
\n | \nInvolved in the transport of activated D-alanine through the membrane | \n\n | \n||
\n \n | \nCell adhesion | \nMediates interactions with components of the extracellular matrix to promote bacterial adhesion | \n\n | \n[158] | \n
\n | \nStress response, response to copper ion | \nRegulation of biofilm formation. May repress cell–cell interaction and cell surface interaction | \n\n | \n[159] | \n
\n | \nStress response to hydrogen peroxide and to DNA damage | \nSingle-species biofilm formation; enhanced flagellar motility | \n\n | \n[160] | \n
\n | \nDNA-binding transcription activator activity | \nThe master regulator for adhesive curli fimbriae expression | \n[161] | \n|
\n | \nDNA-binding transcription factor activity | \nActivates transcription of | \n[162] | \n|
\n | \nNegatively regulates transcription of the | \nDNA-binding protein master regulator of biofilm formation | \n\n | \n[163, 164] | \n
\n | \nProduction of exopolysaccharide | \nBiofilm maintenance | \n[165] | \n|
\n | \n[166] | \n|||
\n | \nRegulatory role. Induces genes involved in biofilm formation | \nDirecting the early stages of colony development | \n||
\n | \nCatalyzes the interconversion between glucose-6-phosphate and alpha-glucose-1-phosphate | \nExopolysaccharide synthesis | \n[167] | \n|
\n | \nBiofilm production under low-nutrient concentrations | \n\n | \n | \n[156] | \n
\n | \nIdentical protein binding | \nMajor component of the biofilm extracellular matrix | \n\n | \n[168] | \n
\n | \nImportant for proper anchoring and polymerization of TasA fibers at the cell surface | \nEssential for biofilm formation | \n\n | \n[169] | \n
\n | \nCleavage of the signal sequence of TasA and TapA | \n\n | \n||
\n | \nConfers a specific microstructure to the biofilm surface | \nConfers hydrophobicity to the biofilm | \n\n | \n[170–171] | \n
\n | \nSynthesis of colanic acid | \nInvolved in the pathway slime polysaccharide biosynthesis | \n\n | \n[172] | \n
\n | \n\n | \n[173] | \n||
\n | \nRegulation of biofilm formation | \nIn the glucose presences, cells showed increased biofilm formation | \n\n | \n[33] | \n
\n | \nMotility-quorum sensing cell proliferation | \nBiofilm architecture | \n[172] | \n|
\n | \nEfflux transmembrane transporter activity | \nRepresses biofilm formation and motility | \n[31] | \n|
\n | \nControls cell motility, size, aggregation, and production of extracellular DNA and extracellular polysaccharides | \nBiofilm dispersal | \n\n | \n[174] | \n
\n | \nSpecific DNA-binding protein | \nMatrix density Cellulose production | \n\n | \n[175–177] | \n
\n | \nLarge surface proteins family | \nBacterial adhesion Biofilm maturation | \n||
\n | \nCell surface-associated protein implicated in bacterial attachment | \nAggregation of unicellular organisms; cell adhesion | \n\n | \n[178] | \n
\n | \nExport of PNAG across the cell membrane | \n\n | \n[149] | \n|
\n \n | \nEnzymes that catalyze the first step in the acetogenesis from pyruvate | \nOrganic free radical synthesis | \n[29] | \n|
\n | \nGlobal regulator of a few genes with important roles in biofilm development | \nBiofilm formation process in a cell density-dependent manner | \n\n | \n[179] | \n
\n | \nInvolved in proteolytic processing | \nQuorum Sensing | \n\n | \n[180] | \n
lmo0048/Putative AgrB-like protein | \nInvolved in proteolytic processing | \n\n \n | \n||
\n | \nHistidine kinase activity | \n\n | \n[181] | \n|
\n Accessory gene regulator | \nA response regulator | \n[182] [183] | \n||
\n | \nProteolytic processing of | \n\n | \n[184] | \n|
luxS/lmo1288/S-ribosyl-homo-cysteine lyase | \nCatalysis of precursor molecules of AI-2 | \n\n | \n[48] [49] | \n|
\n | \nPhospho-relay sensor kinase activity | \n\n | \n
List of genes with significant role in biofilm formation within pathogenic microorganisms (UniprotKB database).
Extracellular and surface proteins such as internalin A and BapL, respectively, have been found to be involved in the initial bacterial adhesion in
\n
In the case of
Moreover,
Microcolonies are formed by cell proliferation, and many genes involved in cell division, cell wall biogenesis, virulence and motility, stress response, and transcriptional regulation factors are expressed.
\n\nTable 1 shows a selection of the genes that are expressed in all the steps of biofilm formation or are upregulated under influence of different biotic or abiotic factors. It was reported that the ∆
The mature biofilm evolves from microcolonies and this development is associated with EPS production. The biofilm matrix of
Furthermore, the structural proteins encoded by
An important polysaccharide identified in the matrix biofilm of many pathogenic bacteria is the colanic acid, which plays an important physiological role for bacteria living in biofilm. This EPS is synthesized by specific enzymes encoded by
Consequently, the transition from the planktonic state to the biofilm state is critical and it is subjected to a strict gene regulation, essential for matrix synthesis, cell aggregation, and cell signaling.
\nNevertheless, bacteria of multiple genetic backgrounds communicate by regulating their relationship of cooperativeness through a mechanism called quorum sensing (QS) in which the bacterial cells are having social interactions with each other through small diffusible signal molecules called autoinducers, thus contributing to the biofilm development [10].
\nQuorum sensing process described in the 1970s is involved in the control of various gene expressions through chemical signaling molecules that are synthesized in response to cell population density [39]. When bacteria start to sense their critical biomass, they answer by activating or repressing genes from 10% of bacteria genome [40]. The system has been described for both Gram-negative and Gram-positive bacteria.
\nAmong QS, other two important regulators are known to control biofilm shape and structure: cyclic diguanosine-5′-monophosphate (c-di-GMP) and small RNAs. For example,
The c-di-GMP involvement in
Gram-positive bacteria such as
The accessory gene regulator of
For
The
Since biofilms act as a barrier that protects the embedded cells against cleaning and disinfecting agents [51], the control of biofilm is an issue that is currently addressed to find effective solutions that can prevent biofilm formation or eliminate the already formed one. Biocontrol of biofilms by using bacteriocins, disruptive enzymes, essential oils, or bacteriophages is gaining importance, as well as using nanoemulsions and nanoparticles. These new methods are promising strategies with remarkable results in the fight against biofilms.
\nBacteriocins are antimicrobial peptides ribosomally produced by an extensive range of bacteria to inhibit or kill competing microorganisms in a micro-ecological system [52, 53]. The most studied bacteriocin and the only one allowed presently as food-grade additive is nisin, a lantibiotic with proven effects against many Gram-positive bacteria including foodborne pathogens [54]. This bacteriocin was shown to penetrate the biofilm formed by
Another way to prevent biofilms development is represented by the adsorption of these bioactive compounds on the surfaces that come into contact with foods [59]. In this case, Nisaplin adsorbed to three types of food-contact surfaces commonly encountered in food processing plants, namely stainless steel, polyethylene terephthalate (PET), and rubber, reduced the adhesion ability of food-isolated
A bacteriocin found to markedly inhibit the biofilm formed by
Disruptive enzymes, such as proteases, glycosidases, amylases, cellulases, or DNAses, are considered a green alternative to chemical treatments often used in the fight against biofilms’ formation in food-related environments [2]. Such enzymes do not have toxic effects and are used both alone and as part of the industrial detergents’ composition to improve their cleaning efficacy [64–66].
\nProteases are a class of enzymes that catalyzes the cleavage of proteins’ peptide bonds. Although they are produced by all living organisms, microbial proteolytic enzymes are preferred over animal or plant origin proteases. The most commonly used source of bacterial proteases is represented by those produced by the genus
Polysaccharide-hydrolyzing enzymes were indicated to remove the biofilms formed by
Plant essential oils (EOs) are rich in phytochemical compounds, which are secondary metabolites produced by plants as defense mechanism against pathogens [74]. Regarding microbial inactivation, EOs have been reported to mainly affect the cellular membrane by permeabilization [75]. This leads to the disruption of vital cellular processes, including energy production, membrane transport, and metabolic regulatory functions [76].
\nStudies evaluating the potential of EOs as disinfectants were conducted. Leonard et al. [77] assessed the bioactivity of
Thyme EO has proven antimicrobial properties [80]. In terms of biofilm inhibition capacity, this EO was shown to inhibit significantly the biofilm formed by
Eugenol is a phytochemical compound preponderantly found in aromatic plants [89]. Interestingly, a study showed that this substance was able to inhibit the intracellular signaling pathway called quorum sensing in the case of biofilms formed by methicillin-resistant
Bacteriophages are viruses that infect bacterial cells. They use the genetic machinery of their host cells to replicate, killing bacteria when reaching a sufficiently high number to produce lysis [92]. They are abundantly encountered anywhere host bacteria live [93] and, therefore, their potential is presently harnessed as natural antimicrobial agents to control pathogenic bacteria in food products and food-related environments [94]. One of the bacteriophages’ applications that is intensively explored targets biofilm-forming bacteria that are relevant for food industry, including
Although it is generally thought that biofilms confer resistance to bacteriophages, these bacterial predators developed several mechanisms to destroy bacteria communities. Once they reach the EPS (extracellular polymeric substances) producing host, they start to replicate, resulting in an increased number and, implicitly, in a progressive degradation of the biofilms and prevention of their regeneration. Bacteriophages can also express or induce the expression from within host genome of depolymerizing enzymes that degrade EPS. Nevertheless, they can also infect persister cells, which are dormant variants of regular bacterial cells that are highly resistant to antibiotics. In this case, the lysis process is triggered once persister bacteria are reactivated [96].
\nScientists [97] reported the ability of a bred phage to reduce L-form biofilms formed by
Currently, controlling biofilm formation by nanotechnology-based antimicrobials is of industrial interest, nanoemulsions and nanoparticles (NPs) with antibiofilm activity being an alternative to conventional methods.
\nRecently, some studies made on model system (polystyrene well plates) and real systems (fresh pineapple, tofu, and lettuce) indicated that nanoemulsions of EOs have significantly higher antibiofilm activity compared to pure EOs (Table 2). Antimicrobial efficacy of nanoemulsions is dependent on the droplet size and electrical properties of nanoemulsions [102, 103], nature of bacteria [75, 104], and food matrix [105–107].
\nNanoemulsion | \nParticle size, nm | \nBiofilm-forming bacteria | \nMode of action | \nRef. | \n
---|---|---|---|---|
EO of | \n73 | \n\n | \nInhibit the ability of bacteria to attach to surfaces | \n[185] | \n
EO of | \n78.46 ± 0.51 | \n\n | \nReduce the adhesion of pathogenic bacteria to surfaces | \n[186] | \n
Trans-CA | \n>100 <100 | \n\n | \nMembrane disruption by destabilization of lipids | \n[187] | \n
Linalool | \n10.9 ± 0.1 | \n\n | \nCell membrane integrity disruption | \n[107] | \n
Antibiofilm activity of essential oil (EO) nanoemulsions.
Nanoparticles (NPs) can be used for both inhibition of biofilm formation and eradication of already formed ones [108].
\nIn the last period, NPs with natural compounds gained increased interest because it was demonstrated that the inorganic capsules can protect the natural products with antimicrobial activity [109]. In this respect, cinnamaldehyde-encapsulated chitosan nanoparticles, garlic-silver NPs, and “tree of tee” oil NPs were used to combat biofilm formation by
Metal-based NPs (silver, gold, and metal oxides) with antimicrobial activity can be used to create different nanocomposite materials able to prevent bacterial adhesiveness to food-contact surfaces and equipment. Wu and coworkers [114] showed that cysteine dithiothreitol and beta-mercaptoethanol were able to reduce
The ability of two types of superparamagnetic iron oxide (IONs and IONs coated with 3-aminopropyltriethoxysilane) to inhibit biofilm formation by
Some food technologies belonging to alternative technologies seem to be successful for preventing the biofilm formation and/or for targeting resistant microorganisms and making them more susceptible to molecular interventions in order to hinder their biofilm formation ability. Among these technologies are included plasma treatments, ultrasound treatments, light-based technologies, pulsed electric fields (PEF), and high hydrostatic pressures. With the exception of ultrasound treatments that can be used to fight against biofilms formed on mechanical parts or pipes, the others are mostly applied for food matrix decontamination.
\nPlasma is generated when the added energy ionizes a gas, which is composed of ions, neutrals, and electrons. Plasma treatment is a surface treatment that has a low penetration depth and was reported to be effective against biofilms, depending on the type of surface biofilms are formed on, the distance between plasma and surface, and the thickness or the microbial load.
\nPlasma sources for producing nonthermal plasma at atmospheric pressure are plasma jets, dielectric barrier discharges (DBD), corona discharges, and microwave discharges. Different other characteristics of the plasma have been reported to influence the biofilms’ inactivation such as the setup (electrode configuration), the exposure mode, the operating gas, the frequency, the plasma intensity (voltage), and the time of exposure [122].
\nResearches [123] showed that the efficacy of DBD in-package atmospheric cold plasma (ACP) against
Govaert et al. [122] studied the influence of different plasma characteristics on the inactivation of
It was shown that ACP is a promising technique but alone cannot achieve complete biofilm inactivation and thus it should be complemented by other surface treatments. Possibility to combine ACP with different biocides such as hydrogen peroxide, sodium hypochlorite, ethylenediaminetetraacetic acid, chlorhexidine, octenidine, and polyhexanide applied before or after the plasma treatment was tested by [126] to reduce biofilms cultivated on titanium discs. Also, Gupta et al. [127] studied the antimicrobial effect of an ACP, plasma jet combined with chlorhexidine, for the sterilization of the biofilms formed by
Ultrasound (US) is a form of energy generated by sound waves at frequencies that are too high to be detected by the human hearing (>16 kHz). The US band is also divided into low frequency (16 kHz−1 MHz) and high frequency (>1 MHz) bands.
\nUS was used as biofilm removal method; however, many studies demonstrated that it should be complemented by other inactivation methods [129, 130]. For example, [130] demonstrated that US removed a significant amount of
Combination of US with mild heat and slightly acidic electrolyzed water was used to test the inactivation of
Synergistic effects were registered also for ultrasound (US; 37 kHz, 380 W for 10–60 min) assisted by peroxyacetic acid (PAA; 50–200 ppm) on reducing
The efficacy of US (37 kHz, 200 W, for 30 min)-assisted chemical cleaning methods (10% alcohols, 2.5% benzalkonium chloride, and 2.5% didecyl dimethyl ammonium chloride) for the removal of
Ultraviolet (UV) light technology is based on the emission of radiation within the ultraviolet region (100–400 nm). The antimicrobial behavior of UV light is based on the formation of DNA photoproducts that inhibit transcription and replication and can lead to cell death [135]. Since the absorption of the DNA is in the 200–280 nm range with the maximum at 254 nm, this wavelength of the UV-C range is called germicidal UV light [136].
\nPulsed light (PL) is the next-generation approach to UV delivery. PL is a technology that can be used to decontaminate surfaces by generating short-time high-energy light pulses (millions or thousands of a second) of an intense broad spectrum (200–1100 nm). PL can be used to decontaminate a great variety of foods as well as to decontaminate contact surfaces, thus improving safety in foods and extending their shelf life [137]. The antimicrobial effect is based on strand breaks that lead to the destruction/chemical modification of the DNA and thus prevent the replication of the bacterial cell [138].
\nRecently, Rajkovic and coworkers [139] evaluated the efficacy of pulsed UV light treatments to reduce
A comprehensive review in the literature underlined the various researches directed mainly at inactivation of pathogens in food or on surfaces and for preventing biofilm formation [137]. While there are often considerable differences in the rate of microbial inactivation by PL, a maximum reduction of 3-log was typically achieved, which is below the reduction performance standard of 5-log required by HACCP regulation [138].
\nRegarding the combined methods, synergistic interaction between gallic acid and UV-A light was able to inactivate
However, PL technology limitation related to the inability to effectively treat uneven food surfaces with crevices, the presence of organic material, and large microbial populations generating shading effects should also be taken into account. Future innovation in PL technology will seek to improve fluence efficiency, for example by considering alternative light sources such as LEDs [141], reflective surfaces included in the treatment chamber, using materials such as titanium dioxide to augment irradiation efficacy [138], and other combination of treatments assisted by PL, based on hurdle approach.
\nPulsed electric field (PEF) is a food processing technology that applies short, high-voltage pulses, across a food material placed between two or more electrodes. The pulses enhance cell permeability by damaging the cell membrane, and if the transmembrane potential is sufficiently high, it produces electroporation. Further, if pores are not resealed, it results in cell death. Most of the food applications are designed for liquid flow through pipes where in a certain region the liquid passes in-between the electrodes area that applies the PEFs [142].
\nThermosonication (TS) was investigated in combination with PEF to determine its effects on inactivation and sublethal injury of
PEF demonstrated synergistic potential in combination with additives (EDTA or triethyl citrate) to inactivate
There is a lot of potential demonstrated by PEF and the combination with different other hurdles could contribute to the elimination of persistent clones able to form biofilms.
\nHigh pressure processing (HPP) is a cutting-edge technology that represents an alternative to conventional processing. HPP has the ability to inactivate microorganisms and enzymes and has a minimal impact on sensorial and nutritional properties of food [145, 146].
\nCombined with other different hurdles, the pressure-assisted processing could be oriented toward a more targeted inactivation of pathogens and prevention of biofilm formation.
\nRecent studies were focused on
However,
Understanding better the molecular mechanisms of stress-related genes will allow to better target pathogen inactivation and to select the right hurdle combination and parameters of unconventional technologies to able to reduce the susceptibility of certain pathogens to form biofilms. These types of studies are just at the beginning and many more researches are expected to focus on these topics in the near future.
\nPathogenic and toxigenic bacteria are able to form biofilms, structures that protect the cells and allow them to remain postsanitation in the food processing environment.
\nSpecific genes are expressed in all the steps of biofilm formation or are upregulated under influence of different biotic or abiotic factors. Genes codify for cell surface structures and appendages (flagella, curli, fimbriae, and pili) that are facilitating biofilm formation by helping bacteria to move toward surfaces and to adhere to them, for extracellular polymeric substances that stabilize the biofilms and protect the cells and for quorum sensing communication.
\nScientists developed novel agents and strategies to control biofilm formation or removal. Their application to the food industry would contribute to eradication of undesirable bacteria from food-processing environments and, subsequently, from food products.
\nThis study was supported by a grant of the Executive Agency for Higher Education, Research, Development and Innovation Funding in Romania awarded to the Dunarea de Jos University of Galati (International and European Cooperation—Subprogramme 3.2—Horizon 2020—Contract no: 15/2017), an institution that is a member of the ERA-IB2 consortium “SafeFood” (ID: ERA-IB-16-014).
\nFlorentina Ionela Bucur’s work has been funded by the European Social Fund through the Sectoral Operational Programme Human Capital 2014–2020, through the Financial Agreement with the title “Scholarships for entrepreneurial education among doctoral students and postdoctoral researchers (Be Entrepreneur!),” Contract no. 51680/09.07.2019—SMIS code: 124539.
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",metaTitle:"Conflicts of Interest Policy",metaDescription:"As an Open Access publisher, IntechOpen is dedicated to maintaining the highest ethical standards and principles in publishing. In addition, IntechOpen promotes the highest standards of integrity and ethical behavior in scientific research and peer-review.",metaKeywords:null,canonicalURL:"/page/conflicts-of-interest-policy",contentRaw:'[{"type":"htmlEditorComponent","content":"In each instance of a possible Conflict of Interest, IntechOpen aims to disclose the situation in as transparent a way as possible in order to allow readers to judge whether a particular potential Conflict of Interest has influenced the Work of any individual Author, Editor, or Reviewer. IntechOpen takes all possible Conflicts of Interest into account during the review process and ensures maximum transparency in implementing its policies.
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\\n\\nAuthors are required to declare all potentially relevant non-financial, financial and material Conflicts of Interest that may have had an influence on their scientific work.
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\\n"}]'},components:[{type:"htmlEditorComponent",content:"In each instance of a possible Conflict of Interest, IntechOpen aims to disclose the situation in as transparent a way as possible in order to allow readers to judge whether a particular potential Conflict of Interest has influenced the Work of any individual Author, Editor, or Reviewer. IntechOpen takes all possible Conflicts of Interest into account during the review process and ensures maximum transparency in implementing its policies.
\n\nA Conflict of Interest is a situation in which a person's professional judgment may be influenced by a range of factors, including financial gain, material interest, or some other personal or professional interest. For IntechOpen as a publisher, it is essential that all possible Conflicts of Interest are avoided. Each contributor, whether an Author, Editor, or Reviewer, who suspects they may have a Conflict of Interest, is obliged to declare that concern in order to make the publisher and the readership aware of any potential influence on the work being undertaken.
\n\nA Conflict of Interest can be identified at different phases of the publishing process.
\n\nIntechOpen requires:
\n\nCONFLICT OF INTEREST - AUTHOR
\n\nAll Authors are obliged to declare every existing or potential Conflict of Interest, including financial or personal factors, as well as any relationship which could influence their scientific work. Authors must declare Conflicts of Interest at the time of manuscript submission, although they may exceptionally do so at any point during manuscript review. For jointly prepared manuscripts, the corresponding Author is obliged to declare potential Conflicts of Interest of any other Authors who have contributed to the manuscript.
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\n\nEditors can also have Conflicts of Interest. Editors are expected to maintain the highest standards of conduct, which are outlined in our Best Practice Guidelines (templates for Best Practice Guidelines). Among other obligations, it is essential that Editors make transparent declarations of any possible Conflicts of Interest that they might have.
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Larramendy",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/14764/images/system/14764.jpg",biography:"Marcelo L. Larramendy, Ph.D., serves as Professor of Molecular Cell Biology at the School of Natural Sciences and Museum (National University of La Plata, Argentina). Appointed Senior Researcher of the National Scientific and Technological Research Council of Argentina. Former Member of the Executive Committee of the Latin American Association of Environmental Mutagenesis, Teratogenesis and Carcinogenesis. Author of more than 450 contributions, including scientific publications, research communications and conferences worldwide. Recipient of several national and international awards. Prof. Larramendy is a regular Lecturer at the international A. Hollaender Courses organized by the IAEMS and former guest scientist at NIH (USA) and the University of Helsinki, (Finland). 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Buchholz",profilePictureURL:"https://mts.intechopen.com/storage/users/89438/images/6463_n.jpg",institutionString:null,institution:{name:"Loma Linda University",institutionURL:null,country:{name:"United States of America"}}}],equalEditorOne:null,equalEditorTwo:null,equalEditorThree:null}]},subseriesFiltersForPublishedBooks:[{group:"subseries",caption:"Plant Physiology",value:13,count:1},{group:"subseries",caption:"Human Physiology",value:12,count:2},{group:"subseries",caption:"Cell Physiology",value:11,count:8}],publicationYearFilters:[{group:"publicationYear",caption:"2022",value:2022,count:1},{group:"publicationYear",caption:"2020",value:2020,count:4},{group:"publicationYear",caption:"2019",value:2019,count:5},{group:"publicationYear",caption:"2018",value:2018,count:1}],authors:{paginationCount:245,paginationItems:[{id:"196707",title:"Prof.",name:"Mustafa Numan",middleName:null,surname:"Bucak",slug:"mustafa-numan-bucak",fullName:"Mustafa Numan Bucak",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/196707/images/system/196707.png",biography:"Mustafa Numan Bucak received a bachelor’s degree from the Veterinary Faculty, Ankara University, Turkey, where he also obtained a Ph.D. in Sperm Cryobiology. He is an academic staff member of the Department of Reproduction and Artificial Insemination, Selçuk University, Turkey. He manages several studies on sperms and embryos and is an editorial board member for several international journals. His studies include sperm cryobiology, in vitro fertilization, and embryo production in animals.",institutionString:"Selçuk University, Faculty of Veterinary Medicine",institution:null},{id:"90846",title:"Prof.",name:"Yusuf",middleName:null,surname:"Bozkurt",slug:"yusuf-bozkurt",fullName:"Yusuf Bozkurt",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/90846/images/system/90846.jpg",biography:"Yusuf Bozkurt has a BSc, MSc, and Ph.D. from Ankara University, Turkey. He is currently a Professor of Biotechnology of Reproduction in the field of Aquaculture, İskenderun Technical University, Turkey. His research interests include reproductive biology and biotechnology with an emphasis on cryo-conservation. He is on the editorial board of several international peer-reviewed journals and has published many papers. Additionally, he has participated in many international and national congresses, seminars, and workshops with oral and poster presentations. He is an active member of many local and international organizations.",institutionString:"İskenderun Technical University",institution:{name:"İskenderun Technical University",country:{name:"Turkey"}}},{id:"61139",title:"Dr.",name:"Sergey",middleName:null,surname:"Tkachev",slug:"sergey-tkachev",fullName:"Sergey Tkachev",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/61139/images/system/61139.png",biography:"Dr. Sergey Tkachev is a senior research scientist at the Institute of Fundamental Medicine and Biology, Kazan Federal University, Russia, and at the Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. He received his Ph.D. in Molecular Biology with his thesis “Genetic variability of the tick-borne encephalitis virus in natural foci of Novosibirsk city and its suburbs.” His primary field is molecular virology with research emphasis on vector-borne viruses, especially tick-borne encephalitis virus, Kemerovo virus and Omsk hemorrhagic fever virus, rabies virus, molecular genetics, biology, and epidemiology of virus pathogens.",institutionString:"Russian Academy of Sciences",institution:{name:"Russian Academy of Sciences",country:{name:"Russia"}}},{id:"310962",title:"Dr.",name:"Amlan",middleName:"Kumar",surname:"Patra",slug:"amlan-patra",fullName:"Amlan Patra",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/310962/images/system/310962.jpg",biography:"Amlan K. Patra, FRSB, obtained a Ph.D. in Animal Nutrition from Indian Veterinary Research Institute, India, in 2002. He is currently an associate professor at West Bengal University of Animal and Fishery Sciences. He has more than twenty years of research and teaching experience. He held previous positions at the American Institute for Goat Research, The Ohio State University, Columbus, USA, and Free University of Berlin, Germany. His research focuses on animal nutrition, particularly ruminants and poultry nutrition, gastrointestinal electrophysiology, meta-analysis and modeling in nutrition, and livestock–environment interaction. He has authored around 175 articles in journals, book chapters, and proceedings. Dr. Patra serves on the editorial boards of several reputed journals.",institutionString:null,institution:{name:"West Bengal University of Animal and Fishery Sciences",country:{name:"India"}}},{id:"53998",title:"Prof.",name:"László",middleName:null,surname:"Babinszky",slug:"laszlo-babinszky",fullName:"László Babinszky",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/53998/images/system/53998.png",biography:"László Babinszky is Professor Emeritus, Department of Animal Nutrition Physiology, University of Debrecen, Hungary. He has also worked in the Department of Animal Nutrition, University of Wageningen, Netherlands; the Institute for Livestock Feeding and Nutrition (IVVO), Lelystad, Netherlands; the Agricultural University of Vienna (BOKU); the Institute for Animal Breeding and Nutrition, Austria; and the Oscar Kellner Research Institute for Animal Nutrition, Rostock, Germany. In 1992, Dr. Babinszky obtained a Ph.D. in Animal Nutrition from the University of Wageningen. His main research areas are swine and poultry nutrition. He has authored more than 300 publications (papers, book chapters) and edited four books and fourteen international conference proceedings.",institutionString:"University of Debrecen",institution:{name:"University of Debrecen",country:{name:"Hungary"}}},{id:"201830",title:"Dr.",name:"Fernando",middleName:"Sanchez",surname:"Davila",slug:"fernando-davila",fullName:"Fernando Davila",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/201830/images/5017_n.jpg",biography:"I am a professor at UANL since 1988. My research lines are the development of reproductive techniques in small ruminants. We also conducted research on sexual and social behavior in males.\nI am Mexican and study my professional career as an engineer in agriculture and animal science at UANL. Then take a masters degree in science in Germany (Animal breeding). Take a doctorate in animal science at the UANL.",institutionString:null,institution:{name:"Universidad Autónoma de Nuevo León",country:{name:"Mexico"}}},{id:"309250",title:"Dr.",name:"Miguel",middleName:null,surname:"Quaresma",slug:"miguel-quaresma",fullName:"Miguel Quaresma",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/309250/images/9059_n.jpg",biography:"Miguel Nuno Pinheiro Quaresma was born on May 26, 1974 in Dili, Timor Island. He is married with two children: a boy and a girl, and he is a resident in Vila Real, Portugal. He graduated in Veterinary Medicine in August 1998 and obtained his Ph.D. degree in Veterinary Sciences -Clinical Area in February 2015, both from the University of Trás-os-Montes e Alto Douro. He is currently enrolled in the Alternative Residency of the European College of Animal Reproduction. He works as a Senior Clinician at the Veterinary Teaching Hospital of UTAD (HVUTAD) with a role in clinical activity in the area of livestock and equine species as well as to support teaching and research in related areas. He teaches as an Invited Professor in Reproduction Medicine I and II of the Master\\'s in Veterinary Medicine degree at UTAD. Currently, he holds the position of Chairman of the Portuguese Buiatrics Association. He is a member of the Consultive Group on Production Animals of the OMV. He has 19 publications in indexed international journals (ISIS), as well as over 60 publications and oral presentations in both Portuguese and international journals and congresses.",institutionString:"University of Trás-os-Montes and Alto Douro",institution:{name:"University of Trás-os-Montes and Alto Douro",country:{name:"Portugal"}}},{id:"38652",title:"Prof.",name:"Rita",middleName:null,surname:"Payan-Carreira",slug:"rita-payan-carreira",fullName:"Rita Payan-Carreira",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRiFPQA0/Profile_Picture_1614601496313",biography:"Rita Payan Carreira earned her Veterinary Degree from the Faculty of Veterinary Medicine in Lisbon, Portugal, in 1985. She obtained her Ph.D. in Veterinary Sciences from the University of Trás-os-Montes e Alto Douro, Portugal. After almost 32 years of teaching at the University of Trás-os-Montes and Alto Douro, she recently moved to the University of Évora, Department of Veterinary Medicine, where she teaches in the field of Animal Reproduction and Clinics. Her primary research areas include the molecular markers of the endometrial cycle and the embryo–maternal interaction, including oxidative stress and the reproductive physiology and disorders of sexual development, besides the molecular determinants of male and female fertility. She often supervises students preparing their master's or doctoral theses. She is also a frequent referee for various journals.",institutionString:null,institution:{name:"University of Évora",country:{name:"Portugal"}}},{id:"283019",title:"Dr.",name:"Oudessa",middleName:null,surname:"Kerro Dego",slug:"oudessa-kerro-dego",fullName:"Oudessa Kerro Dego",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/283019/images/system/283019.png",biography:"Dr. Kerro Dego is a veterinary microbiologist with training in veterinary medicine, microbiology, and anatomic pathology. Dr. Kerro Dego is an assistant professor of dairy health in the department of animal science, the University of Tennessee, Institute of Agriculture, Knoxville, Tennessee. He received his D.V.M. (1997), M.S. (2002), and Ph.D. (2008) degrees in Veterinary Medicine, Animal Pathology and Veterinary Microbiology from College of Veterinary Medicine, Addis Ababa University, Ethiopia; College of Veterinary Medicine, Utrecht University, the Netherlands and Western College of Veterinary Medicine, University of Saskatchewan, Canada respectively. He did his Postdoctoral training in microbial pathogenesis (2009 - 2015) in the Department of Animal Science, the University of Tennessee, Institute of Agriculture, Knoxville, Tennessee. Dr. Kerro Dego’s research focuses on the prevention and control of infectious diseases of farm animals, particularly mastitis, improving dairy food safety, and mitigation of antimicrobial resistance. Dr. Kerro Dego has extensive experience in studying the pathogenesis of bacterial infections, identification of virulence factors, and vaccine development and efficacy testing against major bacterial mastitis pathogens. Dr. Kerro Dego conducted numerous controlled experimental and field vaccine efficacy studies, vaccination, and evaluation of immunological responses in several species of animals, including rodents (mice) and large animals (bovine and ovine).",institutionString:"University of Tennessee at Knoxville",institution:{name:"University of Tennessee at Knoxville",country:{name:"United States of America"}}},{id:"251314",title:"Dr.",name:"Juan Carlos",middleName:null,surname:"Gardón",slug:"juan-carlos-gardon",fullName:"Juan Carlos Gardón",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/251314/images/system/251314.jpeg",biography:"Juan Carlos Gardón Poggi received University degree from the Faculty of Agrarian Science in Argentina, in 1983. Also he received Masters Degree and PhD from Córdoba University, Spain. He is currently a Professor at the Catholic University of Valencia San Vicente Mártir, at the Department of Medicine and Animal Surgery. He teaches diverse courses in the field of Animal Reproduction and he is the Director of the Veterinary Farm. He also participates in academic postgraduate activities at the Veterinary Faculty of Murcia University, Spain. His research areas include animal physiology, physiology and biotechnology of reproduction either in males or females, the study of gametes under in vitro conditions and the use of ultrasound as a complement to physiological studies and development of applied biotechnologies. Routinely, he supervises students preparing their doctoral, master thesis or final degree projects.",institutionString:"Catholic University of Valencia San Vicente Mártir, Spain",institution:{name:"Valencia Catholic University Saint Vincent Martyr",country:{name:"Spain"}}},{id:"125292",title:"Dr.",name:"Katy",middleName:null,surname:"Satué Ambrojo",slug:"katy-satue-ambrojo",fullName:"Katy Satué Ambrojo",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/125292/images/system/125292.jpeg",biography:"Katy Satué Ambrojo received her Veterinary Medicine degree, Master degree in Equine Technology and doctorate in Veterinary Medicine from the Faculty of Veterinary, CEU-Cardenal Herrera University in Valencia, Spain. She is a Full Professor at the Department of Medicine and Animal Surgery at the same University. She developed her research activity in the field of Endocrinology, Hematology, Biochemistry and Immunology of horses. She is a scientific reviewer of several international journals : American Journal of Obstetrics and Gynecology, Comparative Clinical Pathology, Veterinary Clinical Pathology, Journal of Equine Veterinary Science, Reproduction in Domestic Animals, Research Veterinary Science, Brazilian Journal of Medical and Biological Research, Livestock Production Science and Theriogenology. Since 2014, she has been the Head of the Clinical Analysis Laboratory of the Hospital Clínico Veterinario from the Faculty of Veterinary, CEU-Cardenal Herrera University.",institutionString:"CEU-Cardenal Herrera University",institution:{name:"CEU Cardinal Herrera University",country:{name:"Spain"}}},{id:"309529",title:"Dr.",name:"Albert",middleName:null,surname:"Rizvanov",slug:"albert-rizvanov",fullName:"Albert Rizvanov",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/309529/images/9189_n.jpg",biography:'Albert A. Rizvanov is a Professor and Director of the Center for Precision and Regenerative Medicine at the Institute of Fundamental Medicine and Biology, Kazan Federal University (KFU), Russia. He is the Head of the Center of Excellence “Regenerative Medicine” and Vice-Director of Strategic Academic Unit \\"Translational 7P Medicine\\". Albert completed his Ph.D. at the University of Nevada, Reno, USA and Dr.Sci. at KFU. He is a corresponding member of the Tatarstan Academy of Sciences, Russian Federation. Albert is an author of more than 300 peer-reviewed journal articles and 22 patents. He has supervised 11 Ph.D. and 2 Dr.Sci. dissertations. Albert is the Head of the Dissertation Committee on Biochemistry, Microbiology, and Genetics at KFU.\nORCID https://orcid.org/0000-0002-9427-5739\nWebsite https://kpfu.ru/Albert.Rizvanov?p_lang=2',institutionString:"Kazan Federal University",institution:{name:"Kazan Federal University",country:{name:"Russia"}}},{id:"210551",title:"Dr.",name:"Arbab",middleName:null,surname:"Sikandar",slug:"arbab-sikandar",fullName:"Arbab Sikandar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/210551/images/system/210551.jpg",biography:"Dr. Arbab Sikandar, PhD, M. Phil, DVM was born on April 05, 1981. He is currently working at the College of Veterinary & Animal Sciences as an Assistant Professor. He previously worked as a lecturer at the same University. \nHe is a Member/Secretory of Ethics committee (No. CVAS-9377 dated 18-04-18), Member of the QEC committee CVAS, Jhang (Regr/Gen/69/873, dated 26-10-2017), Member, Board of studies of Department of Basic Sciences (No. CVAS. 2851 Dated. 12-04-13, and No. CVAS, 9024 dated 20/11/17), Member of Academic Committee, CVAS, Jhang (No. CVAS/2004, Dated, 25-08-12), Member of the technical committee (No. CVAS/ 4085, dated 20,03, 2010 till 2016).\n\nDr. Arbab Sikandar contributed in five days hands-on-training on Histopathology at the Department of Pathology, UVAS from 12-16 June 2017. He received a Certificate of appreciation for contributions for Popularization of Science and Technology in the Society on 17-11-15. He was the resource person in the lecture series- ‘scientific writing’ at the Department of Anatomy and Histology, UVAS, Lahore on 29th October 2015. He won a full fellowship as a principal candidate for the year 2015 in the field of Agriculture, EICA, Egypt with ref. to the Notification No. 12(11) ACS/Egypt/2014 from 10 July 2015 to 25th September 2015.; he received a grant of Rs. 55000/- as research incentives from Director, Advanced Studies and Research, UVAS, Lahore upon publications of research papers in IF Journals (DR/215, dated 19-5-2014.. He obtained his PhD by winning a HEC Pakistan indigenous Scholarship, ‘Ph.D. fellowship for 5000 scholars – Phase II’ (2av1-147), 17-6/HEC/HRD/IS-II/12, November 15, 2012. \n\nDr. Sikandar is a member of numerous societies: Registered Veterinary Medical Practitioner (life member) and Registered Veterinary Medical Faculty of Pakistan Veterinary Medical Council. The Registration code of PVMC is RVMP/4298 and RVMF/ 0102.; Life member of the University of Veterinary and Animal Sciences, Lahore, Alumni Association with S# 664, dated: 6-4-12. ; Member 'Vets Care Organization Pakistan” with Reference No. VCO-605-149, dated 05-04-06. :Member 'Vet Crescent” (Society of Animal Health and Production), UVAS, Lahore.",institutionString:"University of Veterinary & Animal Science",institution:{name:"University of Veterinary and Animal Sciences",country:{name:"Pakistan"}}},{id:"311663",title:"Dr.",name:"Prasanna",middleName:null,surname:"Pal",slug:"prasanna-pal",fullName:"Prasanna Pal",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311663/images/13261_n.jpg",biography:null,institutionString:null,institution:{name:"National Dairy Research Institute",country:{name:"India"}}},{id:"202192",title:"Dr.",name:"Catrin",middleName:null,surname:"Rutland",slug:"catrin-rutland",fullName:"Catrin Rutland",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/202192/images/system/202192.png",biography:"Catrin Rutland is an Associate Professor of Anatomy and Developmental Genetics at the University of Nottingham, UK. She obtained a BSc from the University of Derby, England, a master’s degree from Technische Universität München, Germany, and a Ph.D. from the University of Nottingham. She undertook a post-doctoral research fellowship in the School of Medicine before accepting tenure in Veterinary Medicine and Science. Dr. Rutland also obtained an MMedSci (Medical Education) and a Postgraduate Certificate in Higher Education (PGCHE). She is the author of more than sixty peer-reviewed journal articles, twelve books/book chapters, and more than 100 research abstracts in cardiovascular biology and oncology. She is a board member of the European Association of Veterinary Anatomists, Fellow of the Anatomical Society, and Senior Fellow of the Higher Education Academy. Dr. Rutland has also written popular science books for the public. https://orcid.org/0000-0002-2009-4898. www.nottingham.ac.uk/vet/people/catrin.rutland",institutionString:null,institution:{name:"University of Nottingham",country:{name:"United Kingdom"}}},{id:"283315",title:"Prof.",name:"Samir",middleName:null,surname:"El-Gendy",slug:"samir-el-gendy",fullName:"Samir El-Gendy",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRduYQAS/Profile_Picture_1606215849748",biography:"Samir El-Gendy is a Professor of anatomy and embryology at the faculty of veterinary medicine, Alexandria University, Egypt. Samir obtained his PhD in veterinary science in 2007 from the faculty of veterinary medicine, Alexandria University and has been a professor since 2017. Samir is an author on 24 articles at Scopus and 12 articles within local journals and 2 books/book chapters. His research focuses on applied anatomy, imaging techniques and computed tomography. Samir worked as a member of different local projects on E-learning and he is a board member of the African Association of Veterinary Anatomists and of anatomy societies and as an associated author at local and international journals. Orcid: https://orcid.org/0000-0002-6180-389X",institutionString:null,institution:{name:"Alexandria University",country:{name:"Egypt"}}},{id:"246149",title:"Dr.",name:"Valentina",middleName:null,surname:"Kubale",slug:"valentina-kubale",fullName:"Valentina Kubale",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/246149/images/system/246149.jpg",biography:"Valentina Kubale is Associate Professor of Veterinary Medicine at the Veterinary Faculty, University of Ljubljana, Slovenia. Since graduating from the Veterinary faculty she obtained her PhD in 2007, performed collaboration with the Department of Pharmacology, University of Copenhagen, Denmark. She continued as a post-doctoral fellow at the University of Copenhagen with a Lundbeck foundation fellowship. She is the editor of three books and author/coauthor of 23 articles in peer-reviewed scientific journals, 16 book chapters, and 68 communications at scientific congresses. Since 2008 she has been the Editor Assistant for the Slovenian Veterinary Research journal. She is a member of Slovenian Biochemical Society, The Endocrine Society, European Association of Veterinary Anatomists and Society for Laboratory Animals, where she is board member.",institutionString:"University of Ljubljana",institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"258334",title:"Dr.",name:"Carlos Eduardo",middleName:null,surname:"Fonseca-Alves",slug:"carlos-eduardo-fonseca-alves",fullName:"Carlos Eduardo Fonseca-Alves",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/258334/images/system/258334.jpg",biography:"Dr. Fonseca-Alves earned his DVM from Federal University of Goias – UFG in 2008. He completed an internship in small animal internal medicine at UPIS university in 2011, earned his MSc in 2013 and PhD in 2015 both in Veterinary Medicine at Sao Paulo State University – UNESP. Dr. Fonseca-Alves currently serves as an Assistant Professor at Paulista University – UNIP teaching small animal internal medicine.",institutionString:null,institution:{name:"Universidade Paulista",country:{name:"Brazil"}}},{id:"245306",title:"Dr.",name:"María Luz",middleName:null,surname:"Garcia Pardo",slug:"maria-luz-garcia-pardo",fullName:"María Luz Garcia Pardo",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/245306/images/system/245306.png",biography:"María de la Luz García Pardo is an agricultural engineer from Universitat Politècnica de València, Spain. She has a Ph.D. in Animal Genetics. Currently, she is a lecturer at the Agrofood Technology Department of Miguel Hernández University, Spain. Her research is focused on genetics and reproduction in rabbits. The major goal of her research is the genetics of litter size through novel methods such as selection by the environmental sensibility of litter size, with forays into the field of animal welfare by analysing the impact on the susceptibility to diseases and stress of the does. Details of her publications can be found at https://orcid.org/0000-0001-9504-8290.",institutionString:null,institution:{name:"Miguel Hernandez University",country:{name:"Spain"}}},{id:"350704",title:"M.Sc.",name:"Camila",middleName:"Silva Costa",surname:"Ferreira",slug:"camila-ferreira",fullName:"Camila Ferreira",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/350704/images/17280_n.jpg",biography:"Graduated in Veterinary Medicine at the Fluminense Federal University, specialist in Equine Reproduction at the Brazilian Veterinary Institute (IBVET) and Master in Clinical Veterinary Medicine and Animal Reproduction at the Fluminense Federal University. She has experience in analyzing zootechnical indices in dairy cattle and organizing events related to Veterinary Medicine through extension grants. I have experience in the field of diagnostic imaging and animal reproduction in veterinary medicine through monitoring and scientific initiation scholarships. I worked at the Equus Central Reproduction Equine located in Santo Antônio de Jesus – BA in the 2016/2017 breeding season. 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