Lipid formulations used for preparation of lipobeads by polymerization within lipid vesicles.
A combination of lipid bilayer and cross-linked polymer network is the logical step in development of polymeric and liposomal nanoscopic systems to provide the natural level of functionality. From liposomal systems, lipobeads borrow the well-developed methods for preparation, diversity of lipids to control the properties of a lipid bilayer, biocompatibility of the lipid bilayer, possibility to vary size and morphology (passive targeting), availability of the external surface for attachment of various ligands (active targeting), encapsulation efficiency of both hydrophilic and hydrophobic molecules. Mechanical stability of the lipid bilayer and environmental responsiveness of the whole structure are the properties that hydrogel core brings to the new construct. The reports reviewed in this chapter demonstrate that lipobeads of nanometer and micrometer sizes can be prepared in different media, retain their stimuli responsiveness under physiological conditions, exhibit both reversible and irreversible aggregation, can be loaded with both small and high molecular weight molecules. As a platform for drug delivery systems, lipobeads have already been loaded with chemotherapeutics, malaria vaccine, and dermatological agent providing different controlled release profiles without leakage. Consecutive multistep triggering, new schemes of drug release, combined drug delivery, vesobeads, proteo-lipobeads, enzyme-containing lipobeads, and hemi-lipobeads are the directions for their future development.
- supramolecular assembly
1. Introduction: why lipobeads?
In accord with our understanding of complex biological mechanisms prevailing
In the last several decades, different terminology, such as supramolecular biovectors (SMBVs), lipid-coated microgels, lipobeads (LB), gel-filled vesicles, lipogels, gel core liposomes, microgel-in-liposomes, hydrogel-supported lipid bilayer, and nanolipogels particles (nLG), has been utilized to describe these lipid membrane/hydrogel structures. In this chapter, we use the term “lipobeads” for the spherical bipartite structures made of a hydrogel core enclosed within a lipid bilayer.
Actually, the bicompartmental structure of lipobeads mimics natural arrangements of living cells. Just look at the cell envelopes for all three main domains of life (
Evidently, the first synthetic lipid vesicles filled with hydrogel (lipobeads) were reported in 1987, when a successful polymerization within liposomes had been accomplished  and microspherules of agarose-gelatin gels filled with gold particles had been mixed with liposomes in the course of their preparation . In 1989, a concept of supramolecular biovectors (SMBVs) was filed as a patent application . The SMBV system was prepared from polysaccharide gel fragments obtained by disruption of a gel of chemically cross-linked maltodextrins and subsequently phospholipidated. In 1994, the SMBVs were reported as new carriers of active substances, such as interleukin-2 (IL-2) . In 1995, lipobeads with Ca-alginate hydrogel core were obtained as a byproduct of a method for the preparation of Ca-alginate hydrogel nanoparticles using the internal compartment of liposomes . In 1996, the spherical hydrogel/lipid bilayer constructs for the first time were named as “lipobeads,” and it was shown that a lipid bilayer was formed on the surface of hydrogel polymer beads upon the addition of phospholipids, if their surface had been modified with the covalently attached fatty acids . Lipobeads with an environmentally sensitive hydrogel core prepared by hydration of lipid films with microgel suspension were described as drug delivery systems in 1998 . In the early 2000s, photopolymerization within liposomes was used for preparation of the so-called synthetic polymer complements with imprinted recognition sites  and the environmentally responsive nanogels . The latter work contributed toward the characterization of the compatibility of nanogels and phospholipid bilayer leading to spontaneous phospholipidation of nanogels . Further studies on lipobeads development were devoted either to new methodologies including different compositions of hydrogel core or different agents which could be loaded into the lipobeads.
The chapter has a comprehensive view on (1) synthetic feasibility, functionality and characterization of lipobeads (Sections 2 and 3) and (2) their potential applications as precursors for novel encapsulated and combined drug delivery systems, as microbiochemical reactors, and as an experimental model to study the origin of life (Section 4). It is a useful source of references for the researchers from both academia and industry, who deal with the aforementioned areas of applications. It may be predicted that in the future, the demand in this information will rise dramatically because of a growing interest, especially, in the encapsulated drug delivery systems with tiny bioscopic mechanisms of drug release.
2. Strategies of lipobeads preparation
Two general methods available to date for preparation of artificial bilayer-coated hydrogel particles (lipobeads, giant, or nano) are sketched in Figure 2.
The first one (Figure 2A) uses the liposomal interior as a chemical reactor for the formation of hydrogel by polymerization [1, 8–22]. In this method, lipid bilayer should be sealed enough to retain the concentration of pregel components and strong enough to withstand the steps preventing polymerization in the surrounding medium.
The second one (Figure 2B) is based on the formation of lipid layers around hydrogels after microgel/liposome mixing. In this case, lipid bilayer adsorption on the surface of hydrogel particles prepared separately was promoted via Coulombic attraction between the charged microgels and oppositely charged lipids [7, 23, 24], hydration of lipid films with micro- or nanogel suspension [2, 3], introduction of hydrophobic anchors at the microgel surface around which adsorbing lipids may assemble [6, 25–28], centrifugation of microgels onto a lipid film , microfluidic flowing , and emulsification [30–32].
In both cases, it is the stability, fluidity, and permeability that are the main properties of a lipid bilayer, which should be governed in the course of lipobeads engineering.
2.1. Effectors of the lipid bilayer fluidity, stability, and permeability
Stability and permeability of a lipid bilayer depend on its fluidity, which can vary with temperature and phospholipid composition. Bilayers undergo a change from liquid to gel (solid) state at the so-called lipid (or order-disorder) phase transition temperature (
Therein, the shorter the length of hydrocarbon chains, the lower is the
In the liquid-crystalline “disordered” state, the membrane is fluidic, namely: (i) both alkane chains and head groups of phospholipids are more flexible than in the solid “ordered” state, (ii) the area lipids occupy becomes greater by changing from a 0.48 nm2/head group to 0.7 nm2/head group, that is, bilayer expands, (iii) lateral diffusion of phospholipids in the plane of the bilayer and rotation of lipid molecules around C─C bonds accelerate, and (iv) transverse “flip-flop” migration of lipids from one monolayer to the other side of the bilayer becomes more probable.
The factors affecting the lipid phase transition temperature are crucial for lipobeads engineering. Indeed, one can expect the lipid bilayer to be more elastic (favorable for formation of unilamellar membrane) and less sealed (unfavorable for gelation within liposomal interior) above
2.2. Polymerization within liposomal nano-/microreactors
In general, preparation of lipobeads using vesicle interior as microreactors includes a number of crucial steps, as shown in Figure 2A.
First of all, encapsulation of hydrogel-forming components into liposomes can result from hydration of lipid cast film formed upon solvent evaporation [1, 2, 5, 9, 11, 13, 14, 16, 32], electroformation [12, 22, 33, 34], or rapid phase evaporation [19, 20]. The size of liposomes ensures the final size of lipobeads. Depending on the size, lipobeads can be classified into two groups: nanolipobeads (<1000 nm) and giant lipobeads (>1 μm). Nanolipobeads should be used in realistic drug delivery systems, whereas giant lipogels permit a direct study of structural functionality of the hydrogel/lipid bilayer assemblies, cargo loading, and release mechanisms  using optical microscopy.
For 100-nm lipobeads, the liposomal formulations have to be sonicated [1, 9] or extruded through a nanopore filter of a needed pore size [5, 8, 11, 14, 16, 19, 32]. Another approach to control the size of pre-lipobeads is based on hydrodynamic focusing of a stream of the liposome precursor solution by the flow of a hydrogel-forming solution within a microfluidic device . Although the microfluidic-directed approach and electroformation are very elegant methods, they are hardly suitable for a scaled production of lipobeads with regards to pharmaceutical applications.
If the ultimate goal was to engineer the giant lipobeads greater than 1 μm, the gentle hydration of a phospholipid cake [17, 36] or a hybrid agarose/lipid film [unpublished], electroformation [12, 22, 33, 34], emulsification , and reverse phase evaporation [1, 13, 30] were used without any agitation of the lipid formulations.
Typical lipid formulations for preparation of lipid vesicles filled with the hydrogel-forming solution are shown in Table 1.
|Lipid formulation||Method for prelipobead preparation||Size||Ref.|
|Main phospholipid||Other components|
|EPC||−10°C||Cholesterol||Reverse phase evaporation, sonication||~600 nm|||
|—||Lipid film hydration, sonication||~150 nm|||
|—||Detergent removal or extrusion||~200 nm|||
|Cholesterol||Reverse phase evaporation||~1–2 μm|||
|—||Lipid film hydration, extrusion||~100 nm|||
|0.2–100 μm||[22, 34]|
|DPPC||42°C||—||Lipid film hydration, extrusion||~800 μm|||
|Cholesterol/DCP||Hydrodynamic focusing in microfluidic device||150–300 nm|||
|SOPC, neutral||6°C||DOTAP, positively charged||Lipid film hydration, extrusion||~350 nm|||
|Soybean polar lipid extract: PC (45.7%), PE (22.1%), PI (18.4%), PA (6.9%), others (6.9%)||?||–||Lipid film hydration||10–50 μm|||
|HSPC||52°C||Cholesterol||Lipid film hydration||2–200 μm|||
Second, when a suspension of vesicles filled with the hydrogel-forming solution is prepared, it is important to prevent cross-linking or polymerization outside those vesicles. This has been done by several methods, such as a 5- to 20-fold dilution [9, 11, 12, 14, 16, 22, 33, 34], gel filtration [1, 21], centrifugation and dialysis [5, 13, 20, 30], or introduction of polymerization scavengers (e.g., ascorbic acid ) into the extravesicular space. In addition, hydrogel-forming solution as well as cross-linker and initiator can be microinjected directly into the internal compartment of a giant unilamellar phospholipid vesicle (GUV) .
The third step is gelation of the hydrogel-forming solution entrapped within the closed lipid bilayer. Thermal and ionotropic cross-linking are the examples of physical cross-linking reactions. Some hydrogel cores were made of polysaccharides when temperature changes [2, 5, 32]. Indeed, agarose  and κ-carrageenan  are the temperature-sensitive polysaccharides which structure in aqueous solutions undergoes a transition from a random-coil conformation to the cross-linked double helixes upon cooling. Agarose is not biodegradable, but its combination with gelatin can bring biodegradability . Gelatin is a thermoresponsive protein, forming a reversible cross-linked network by cooling a water-based solution of the polymer below 35°C. The hydrogel can be liquefied by heating it to physiological temperatures. Interestingly, κ-carrageenan, an anionic polysaccharide carrying one sulfate group, can be cross-linked both thermally (upon cooling) and ionotropically in the presence of divalent or monovalent cations . Similar to alginate, the degradation of carrageenan hydrogels is driven by the exchange of ions with the surrounding fluids. In the course of ionic cross-linking within interior of vesicles, the sections of the polymer backbone carrying the charge bind with ions of opposite charge. For example, when multivalent cations (e.g., Ca2+) were added to a water-based alginate  or poly(ethylene dioxythiophene)/poly(styrene sulfonate)  solutions, they bound adjacent polymer chains forming ionic interchain bridges that caused a cross-linking. The pH-driven cross-linking inside liposome was carried out by lowering the pH of aqueous solution of poly(acrylic acid) carrying carboxyl groups [20, 30].
The greatest portion of works on gelation within liposomal reactor used photopolymerization to generate a strong covalently cross-linked hydrogel [1, 9, 11, 14, 16, 22, 33, 34, 39]. The monomer and cross-linker depends on the hydrogel core properties required for different applications, as shown in Table 2.
|Monomer.||Cross-linker||Initiator||Prevention of macroscale gelation||Property of hydrogel core||Ref.|
|PAAm||MBA||ACVA + TEMED||GPC||Not specified|||
|Anchored and nonanchored PNIPA-VI||MBA||DEAP||Dilution 25-fold||Temperature and pH sensitivity|||
|Anchored PNIPA||TEGDM||DEAP||Dialysis||Temperature sensitivity (probably)|||
|PNIPA or PAAm||MBA||DEAP||Dilution 20-fold||Temperature sensitive|||
|PNIPA||MBA||DEAP||Gel filtration||Temperature sensitivity|||
|dex-HEMA||HEMA||IC2959||Dilution 10-fold||Enzymatically degradable (dextranase)|||
|PAA||MBA||IC2959||Radical scavenging by AA||pH sensitivity (probably)|||
|PNIPA||MBA||DEAP||Dilution||Temperature, pH, pI sensitivity||[22, 33, 34]|
|PAAm||MBA||DEAP||Dilution 20-fold by glucose solution (2.8 M)||Enzymes entrapment, storage, protection, and release|||
Finally, the formulation has to be washed from unreacted chemicals using centrifugation and/or dialysis. In the course of this step, the required medium external to the lipobeads can be introduced. For example, lipobeads could be dispersed in distilled water [13, 30, 36], buffers with pH varied from 7.0 to 7.8 [1, 8, 9, 11, 14, 16, 17, 19–21, 30], or aqueous solutions of sucrose [12, 24, 33, 34]. If necessary, the prepared lipobeads can be dried by gentle evaporation in temperature gradient to be stored at 4°C.
2.3. Hydrogel/liposome mixing
From the general scheme of hydrogel/liposome mixing (Figure 2B), one can conclude that hydrogel particles and liposomes should be prepared separately, therein the final size of lipobeads will be defined by the size of hydrogel particles prepared before mixing with liposomes. It has been demonstrated microscopically [40, 41] that hydrophobic modification of the nanogels is not required for spontaneous formation of the bilayer on their surface. Together with the other studies , these findings presume that hydrogel/lipid bilayer is an energetically favorable structure.
Lipid formulations used for preparation of lipobeads by hydrogel/liposome mixing are systemized in Table 3. Phospholipid with different
|Lipid formulation||Lipid vesicles preparation||Medium (pH)||Ref.|
|Main phospholipid||Other components|
|Cholesterol/triolein||w/o emulsion, organic solvent evaporation||5% dextrose HEPES (pH 7.4)|||
|Cholesterol||Injection of ethanol solution of PL in water, homogenization||PBS (pH 7.4)||[3, 4, 40]|
|EPC||−10°C||–||Lipid film hydration, sonication or extrusion||HEPES (pH 7.4)|||
|–||Tris (pH 7.0)|||
|EPC/DMPE||−10°C/50°C||Cholesterol||HEPES (pH 7.0)|||
|EPC||−10°C||PS/cholesterol||HEN (pH 7.4)|||
|DMPC/DPPC||24°C/42°C||–||PBS (pH 7.4)|||
|EPC||−10°C||PS/cholesterol||HEN (pH 8.0)|||
|PE||63°C||Olein oil/cholesterol||HEPES (pH 7.4)|||
|POPC||−2°C||–||TRIS (pH 7.2)||[28, 48]|
|DOTAP(+)||HEPES (pH 7.6)|||
|HSPC||52°C||Cholesterol||Injection of ethanol solution of PL in hot water, sonication||Water||[37, 42]|
There are only a few reports (Table 4) that deal with nanogels to prepare lipobeads on the nanometer scale: one group employed a high pressure homogenizer to crash bulk polysaccharide hydrogel down to nanosized particles [39, 42], the other group used nanogels extracted from liposomal reactors [9, 10, 26, 27]. In principle, emulsion polymerization enables preparation of hydrogel particles with a diameter less than 150 nm. However, there is a problem of complete removal of the residual materials. In the absence of an added surfactant, the method is called precipitation polymerization. With the latter two methods, the lipobeads of 1-μm diameter are produced [2, 7, 23, 25, 28, 31]. To prepare giant lipobeads with a diameter up to 100 μm, the inverse suspension polymerization (ISP) method is commonly applied [6, 24, 35, 43–45].
|Hydrogel bead composition||Method of bead preparation||Size||Property of hydrogel beads||Ref.|
|Agarose-gelatin cross-linked by cooling||Emulsification||~1–5 μm||Degradable|||
|Cross-linked polysaccharide fragments||Extrusion in high-pressure homogenizer||30–60 nm||Temperature, pH, pI: core/bilayer interactions|||
|Acylated PVA cross-linked by freeze-thaw||ISP||1–100 μm||–|||
|PMAA-NPMA cross-linked by MBA||ISP||~6 μm||pH sensitivity|||
|Anchored PDMAA cross-linked by E-BIS||ISP||5–600 μm||–
Antibody-antigen interaction 
|dex-HEMA-MAA (−) or dex-HEMA-DMAEMA (+)||Emulsification||2–5 μm||Degradable|||
|Acylated PVA cross-linked by freeze-thaw||ISP||~80–100 μm||–|||
|Anchored PNIPA/P(NIPA-AA) core-shell cross-linked by MBA||Precipitation polymerization||~0.3–2 μm||Temperature, pH sensitivity||[25, 28]|
|Sodium hyaluronate-PEGDA||Emulsification||1–15 μm||Drying/wetting|||
|PAAm-allylamine (+) or PAAm-AMPS (−) cross-linked by MBA||ISP||1–100 μm||Electrostatic liposomes/MG interactions|||
Liposomes can be brought into contact with hydrogel particles by mixing hydrogel particles and liposomes, addition of hydrogel particles into dried lipid film before hydration or hydration of the lipid film by aqueous suspension of hydrogel particles. The formation of lipid bilayer around hydrogel particles can be enhanced by shaking, vortexing, pipette agitation, centrifugation, freezing-thawing, heating-cooling, or their combination. The fusion of liposomes will be more advanced at temperatures higher than the
2.4. Modification of hydrogel core and lipid bilayer
To the great extent, the major methods for lipobeads’ synthesis (polymerization within liposomal interior and liposome/hydrogel mixing) and further functionalization are analogous to those used for engineering their compartments—conventional nanogels and lipid bilayers.
2.4.1. Hydrogel core
Polymeric nanogels can be synthesized by three straightforward methods: (i) cross-linking polymer chains within already formed nanoparticles using, for example, emulsion polymerization technique [47–49], (ii) polymerization within the liposomal interior followed by the lipid bilayer removal , and (iii) photolithographic fabrication of submicrometer hydrogel particles using the PRINT technique [50, 51] or step and flash imprint lithography (S-FIL)  as an alternative nanoimprint photolithographic approach.
To engineer the stimuli-responsive nanogels (Figure 4), a molecule of interest can be conjugated to the polymer network through a cleavable tether, so that when the tether is cleaved, the drug is allowed to diffuse into the nearby medium. Alternatively, if different molecules are trapped within an environmentally sensitive polymer network with or without environmentally responsive cleavable linkers, the network either changes its volume (swells/shrinks) or degrades when the environmental conditions change, allowing the molecules to be released. For example , the doxorubicin-loaded, pH- and redox-sensitive poly(oligo(ethylene glycol) methacrylates-ss-acrylic acid) nanogels exhibited strong internalization by human hepatocellular carcinoma cells (Bel7402) under reduced opsonization and phagocytosis. Herein, the intracellular glutathione (GSH) triggered the release of doxorubicin from the nanogels into cytosol for subsequent entering the nucleus.
2.4.2. Lipid bilayer
Specific functionality and entrapment of both hydrophilic and hydrophobic molecules into a liposomal interior are the two main objectives of the liposomal system development. Eventually, four types of liposomal systems can be distinguished (Figure 5), namely: classical “plain” liposomes, “stealth” liposomes, ligand conjugated liposomes, and stimuli-sensitive liposomes.
The studies on “plain” (traditional) liposomes revealed the difficulty in loading of some types of molecules and leakage of contents from the liposomal interior [54–58]. The further development of the “plain” liposome systems aimed at overcoming these obstacles. In particular, to reduce leakage from liposomes, phospholipids with a higher phase transition temperature  were used, and cholesterol  and sphingomyelin  were incorporated into the lipid bilayer to make it more solid at temperatures of application.
Loading and retention of molecules of interest within liposomes are the molecule dependent processes. For example, weak bases were loaded in response to pH gradients [62–66]. Some molecules, such as doxorubicin, exhibited good retention properties under conditions enhancing their precipitation inside liposomes [67–69], whereas retention of highly hydrophobic molecules, like paclitaxel, was still a challenge [70, 71] until they were converted into the weak bases .
Furthermore, the pharmaceutical studies revealed that (i) serum proteins effected on release of drug molecules entrapped into liposomes, (ii) liposomes were cleared very rapidly from circulation by uptake into the cells of the mononuclear phagocyte system (MPS), predominantly in the liver and spleen [73, 74], and (iii) there existed both cellular and intracellular barriers to liposomal delivery . The so-called “stealth” liposomes were developed by stabilizing liposomes with protective polymers (e.g., polyethylene glycol, PEG)  in order to increase their circulation time within a biologically active environment, such as blood. In addition, the clearance of liposomes from the body was found [74, 80] to be slower, if they contained neutral or slightly negative phospholipids and their size was around 100 nm. Therefore, the rate of molecules’ release should be optimized [77–79].
Ligand-conjugated liposomes were built to target specific cells, intracellular organelles, tumor microenvironment, and/or facilitating receptor-mediated endocytosis (attachment of antibodies, folate, transferrin, tyrosine kinase, vascular endothelial growth factor, introduction of fusogenic lipids, and membrane-active peptides). In particular, three ways to facilitate the intracellular drug delivery include (i) introduction of fusogenic lipids or membrane active peptides into liposomal bilayer enhances fusion or even disruption of cell/organelle membrane and thereby improves cytoplasmic delivery of drug [81–85], (ii) utilization of macrophages for natural endocytosis of drug-loaded liposomes , and (iii) receptor-mediated endocytosis of ligand-targeted liposomal drug carriers into the intracellular compartment (see reviews [87–90]).
The release of liposomal contents can be triggered either remotely by heat, radiation, and ultrasound or locally by pH, enzymes, and redox triggers (see review  and references therein). For these purposes, the lipid bilayer can be modified with stimuli-sensitive phospholipids, polymers, cleavable tethers, and linkers, as shown in Figure 5. Recently, the lipid bilayer consisting of molecules of the temperature-sensitive phospholipid 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (MSPC) and decorated with gold nanorods was shown to become more permeable for the pain blocking molecules (tetrodotoxin) without a tissue burn when exposed repeatedly to a low intensity near-infrared irradiance .
2.5. Encapsulation strategies
2.5.1. Loading during gelation within liposomal interior
Unfortunately, little work has been done so far to test loading capability of lipobeads prepared by polymerization within the lipid vesicles [11, 14, 15, 20, 30]. Typically, a hydrophobic cargo was either incorporated within lipid bilayer at the step of lipid vesicle formation or copolymerized with hydrogel core as an anchor, whereas a hydrophilic cargo was added as a component of the hydrogel-forming solution and incorporated into the intravesicular space before gelation started [9, 11, 13].
The main challenge of the scheme when a load is introduced into the aqueous phase followed by rehydration of a lipid film and further polymerization within a liposomal reactor might be the damage to the loading molecules by toxic ingredients of the hydrogel-forming solution (if any) and/or high temperature and UV radiation initiating polymerization. This approach can be especially problematic for encapsulation of proteins, because of denaturation. Nonetheless, it has been reported that antigen model (BSA)  or combination of protein antigen (Pfs25) and oligonucleotide sequence (CpGODN) encapsulated into pH-cross-linked PAA hydrogel core of lipobeads remain intact and active . Moreover, encapsulation efficiencies of lipobeads were shown to be by 10% higher than those for liposomal carriers. A high encapsulation efficiency of lipobeads has been demonstrated also for hemoglobin [14, 15], which withstands the conditions of free radical polymerization and UV radiation. The other example of successful coencapsulation of hydrophilic proteins (IL-2) and small hydrophobic molecules (TGF-receptor-I inhibitor, SB505124) into the biodegradable hydrogel core of lipobeads has been presented in : encapsulation efficiencies were 80 and 36%, respectively, and UV polymerization did not compromise bioactivity of both immunomodulators. Recently, PAAm lipobeads with a good encapsulation efficiency (37%) of enzymes (bovine Cu, Zn-superoxide dismutase, and bovine milk lactoperoxidase) were synthesized to prove that the UV irradiation used for interior gelation did not cause any reduction in the enzymatic activity of the proteins .
2.5.2. Loading of lipobeads prepared by hydrogel/liposome mixing
In the case of lipobeads prepared by hydrogel/liposome mixing, hydrophilic cargos usually are introduced into the interior of hydrogel particles, whereas hydrophobic ones—into the lipid bilayer of liposomes before their mixing. Some molecules can penetrate through the lipid bilayer into the hydrogel core.
Back in 1987, the 200–600-nm agarose-gelatin nanogels were filled with colloidal gold particles prior to mixing with liposomes to form lipobeads . Since colloidal gold particles are very adsorptive for proteins and peptides, their encapsulation into hydrogel core can increase the loading capacity of lipobeads. The cytokine Interleukin-2 (IL-2) plays an important role as an immunostimulator and can be relevant as a treatment by itself for cancer and HIV. However, the difficulties faced today with IL-2 are its toxicity and short half-life. To resolve these problems, this protein was bound to the lipobeads (polysaccharide hydrogel nanoparticles coated with lipid bilayer) [4, 93]. In principle, the lipobeads can be loaded with a number of entities just by their incubation in the corresponding solutions, for example, Ca2+ ions and drug mimicking molecules , adenosine triphosphate (ATP) , and dextran (1.5–3.0 kDa) . Herein, it was found that permeability of the lipid membrane was similar to the free bilayer. To incorporate transmembrane proteins into the peripheral membrane of lipobeads, the hydrogels core particles were mixed and incubated with liposomes containing the proteins of interest within their lipid bilayer .
The only chemotherapeutic drug—doxorubicin—was loaded into lipobeads [6, 43]. Encapsulation was performed before lipobeads formation by soaking the dry hydrogel particles in a drug-dissolved solution. The drug diffuses inside in the course of the polymer network swelling and mesh size increase. Further mixing of hydrogel particles with liposomes encapsulates the drug into the lipobeads. As a result, the unbelievably high doxorubicin concentration of ~2 M, which is 10-fold the concentration in liposomes , was achieved.
A lecithin-based microemusion method was proposed for fabrication and loading of single-core or multicore lipobeads . The loading efficiency of caffeine into thus prepared sodium hyaluronate lipobeads was obtained to be 30%. A concentration of natural moisturizing factor close to the one present in corneocytes (15%) was encapsulated into the lipobeads, which acquire an enhanced water retention ability similar to corneocytes. This makes them potential for applications in cosmetics and dermatology.
Encapsulation of a protein drug into hydrogel particles before lipobeads formation can be performed either by formation of a hydrogel particle in the presence of a protein drug or by incubation of the preformed hydrogel particles in a protein solution. The first approach again could be problematic due to a danger of protein denaturation. The second approach is limited by the size-exclusion effect resulting in a lower loading concentration of proteins. However, encapsulation of proteins into microgels is a promising tool to increase the amount of drug loaded in a prelipobead (loading capacity) by using the “intelligent” properties of polymer networks (swelling/shrinking ability in response to stimuli) .
3. Hydrogel core swelling/collapsing and lipobeads properties
The three-dimensional polymer network within a closed lipid bilayer (liposome) can be considered as a gigantic single molecule stabilized by chemical (covalent bonds) or/and physical (ionic bonds, entanglements, crystallites, charge complexes, hydrogen bonding, van der Waals, or hydrophobic interactions) cross-links. The hydrogel core is also an open container with semipermeable boundaries, across which water and solute molecules can move whereas charged (ionizable) groups fixed on the network chains cannot move (Figure 4). Herein, the network of cross-linked polymers exhibits both liquid-like and solid-like behavior [96–99]. Therefore, because of its high water content and elastic nature similar to natural tissue, hydrogel core within a liposome is solid enough to support lipid bilayers and liquid enough to keep the membrane intact and functional.
Besides the mechanical stability that hydrogel core provides to the lipid bilayer, the stimuli-sensitivity of the polymer network can be used for managing the environmental responsiveness of lipobeads.
3.1. A variety of possible hydrogel cores
Depending on the composition of a gel/solvent system, the polymer and cross-linking chemistry, nanogels swell or shrink discontinuously or continuously, reversibly or irreversibly in response to many different stimuli (temperature, pH, ion concentration, electric fields, light, reduction/oxidation, enzymatic activity, etc.) [47, 100–105]. In general, various types of hydrogels based upon either natural (e.g., hyaluronic acid, collagen, chondroitin sulfate, alginates, fibrin, and chitosan) and synthetic polymers made of neutral (e.g., 2-hydroxyethyl methacrylate, N-alkylmethacrylamides, N-alkylacylamides, and N,N-dialkylacrylamides), acidic (e.g., acrylic acid, metacrylic acid, and 2-acrylamido-2-methyl propane sulfonic acid), or basic (e.g., N,N-dialkylaminoethyl methacrylate, 1-vinylimidasole, and methacryloyoloxyethyltrialkylammonium bromide) monomers have been prepared, studied, and used in numerous applications (bioseparation, tissue engineering, sensing and molecular recognition, drug and gene delivery, controlled release, artificial muscles, and flow control).
3.1.1. Temperature-sensitive volume change
Typically, the thermoresponsive hydrogels are classified as having either positive or negative volume phase transition with a characteristic temperature (
Thus, first and foremost, a temperature range where the hydrogel shrinks or swells intrinsically depends on the chemical nature of the polymer constituting its network. Herein, the volume changes in a water-swollen hydrogel can be either continuous or discontinuous, as a function of environmental stimuli. If the system remains totally miscible at given thermodynamic conditions, one can expect continuous volume transition. On the contrary, if changes in chemical nature of the polymer network, solvent quality, or environmental stimulus “push” the system into a two-phase (unstable) region of the solubility phase diagram, one can expect that properties of the hydrogel, most notably its volume, change discontinuously. In addition, the studies [115–117] show that increased cross-linking may significantly decrease swelling ability of hydrogel, especially, below
Figure 6 demonstrates that temperature-sensitive shrinking ability of hydrogels depends on their microstructure and method of preparation: the granular hydrogel (A) exhibits a continuous volume decrease with temperature, whereas the denser hydrogels (B and C) exhibit more abrupt changes in volume within the 35–45°C range. Interestingly, the granular structure of the PNIPA hydrogels prepared by thermal polymerization in water (A) can be broken down into separate submicroscopic domains by means of sonication (data not shown). On the contrary, sonication does not affect the structure of “dense” hydrogels prepared either by thermal polymerization in dimethyl sulfoxide (DMSO) (B) or by photopolymerization in water (C).
It has been shown  that incorporation of a small amount of ionizable groups into the nonionic gel network drives the volume phase transition from continuous volume changes toward discontinuous one (Figure 7). Moreover, an increase in the portion of sodium acrylate with carboxylic groups on the PNIPA network allowed one to vary the
3.1.2. Ionic sensitivity of hydrogels
Figure 7 also suggests that incorporation of charged (anionic, cationic, or both) groups on the polymer network makes the volume transition temperature and degree of swelling dependent on pH and ionic strength. Indeed, the poly(N-isopropylacrylamide-co-methacrylic acid) (PNIPA-MAA) microgel particles  at pH 3.4 exhibited a decrease in
The type of ionizable groups on the polymer networks makes the maximum swelling ability of a gel strongly dependent on pH. For example, the anioic PNIPA-MAA microgels exhibited the maximum swelling in the range of pHs from 6.5 to 10 , whereas for the cationic PNIPA-VI nanogels, the maximum swelling ratio was observed in the range of pHs from 6 to 3.5 . It becomes even more intriguing if the so-called polyampholyte hydrogels are designed  by addition of both cationic (VI) and anionic (AA) groups on the network. The polyampholyte gel was in a shrunken state near the isoelectric point (pH ~ pI), and it swelled at both higher and lower pHs. It is interesting that such designed polyampholyte gels can work like biochemomechanical systems in which the enzymatically induced pH changes control the volume of polyampholyte network or, in opposite direction, the pH sensitive volume changes control the activity of enzymes immobilized into the gel .
There are experimental evidences [126–130] that different monovalent (Li+, Na+, K+, and Cs+) and divalent (Ca2+, Mg2+, Sr2+, and Ba2+) ions are able to promote deswelling effects in hydrogels of different chemical nature. Interestingly, at the same molar ratios of divalent to monovalent cations (~1 mM/30 mM), the similar volume changes were observed in biological polyelectrolyte systems during physiological processes like nerve excitation, muscle contraction, and cell locomotion [131–137].
3.1.3. Surfactants as effectors of hydrogel volume change
The extensive theoretical and experimental [138–145] studies have shown that the addition of anionic, cationic, and nonionic surfactants to the solution containing a gel can also influence the
3.1.4. Light-sensitive hydrogels
Photosensitive hydrogels with incorporated photosensitive molecules into the gel network have been reported as well. For example, the gels with incorporated leucocyanide and leucohydroxide  underwent volume changes upon irradiation and removal of ultraviolet light resulted from ionization reaction and internal osmotic pressure initiated by UV light. Significant volume changes in hydrogels were also induced by visible light . However, the mechanism of volume transition was different—it was due to direct heating of the polymer network by light. Nevertheless, more recent reports  showed that a focused laser beam was able to induce reversible shrinking in polymer gels due to radiation forces, rather than local heating, modifying the weak interactions in the gels. Herein, gel shrinkage was observed up to several tens of micrometers away from the irradiation spot. The light-induced contraction was also found in acrylamide gels, which are not temperature sensitive. In hydrogels with temperature-sensitive volume phase transitions, such as PNIPA gel, it was found that the radiation force of the laser beam not only induces the volume phase transition but also lowers the transition temperature
The fact that the volume change initiated by light is extremely fast seems of great importance for the development of the light-sensitive lipobeads. One could predict that the photosensitive hydrogel cores will also receive an increasing scientific and technological attention due to their capability of serving as the so-called shape-memory polymeric systems . Being exposed to the light with lower wavelengths, the shape-memory materials become deformed and their temporary shape is fixed due to cross-linking. When irradiated with higher wavelengths, they recover their initial shape because of the cross-links cleavage.
3.1.5. Electrical field-induced volume change
Back in the 1950s, it was found that contraction, oscillation, and bending of polyelectrolyte gels can be induced electrically [150–152]. In particular, gels prepared from polymers and copolymers that contain ionizable groups exhibited remarkable contraction when placed between a pair of electrodes connected to a direct current source. Polymer gels containing no ionizable groups showed no volume change under electrical field applied. The extent and rate of volume change of the polyelectrolyte gels were shown to increase with increasing electrical field . An increase in the ionic strength (e.g., an addition of NaCl) also increases the rate of gel shrinkage, whereas an addition of organic solvent (e.g., acetone or ethanol) decreases both the extent and the rate of shrinking. In different types of hydrogels, an electrically activated volume changes were associated with the induction of the medium pH change by the electric field , the electrically initiated volume phase transition , and the so-called electrokinetic phenomena—ion transport of counter-ions in the electric field .
3.2. Lipobeads by hydrogel/liposome mixing
Success in formation of lipobeads by hydrogel/liposome mixing (see references from Tables 3 and 4) is an experimental confirmation of the main property of hydrogel and lipid bilayer—their compatibility. Indeed, the phospholipid bilayer spontaneously self-assembled around a nanogel once extracted from a lipobead (Figure 8a) and mixed with liposomes (Figure 8b) to form a secondary lipobead (Figure 8c) .
Moreover, spontaneous formation of the lipid bilayer on the surface of nonanchored microgels was shown microscopically for liposomes made of different phospholipids with or without cholesterol [36, 41]. Figure 9 represents both laser scanning confocal (Figure 9A, A′, A”) and scanning electron microscopy (Figure 9B, B´) images evidencing the fusion of liposomes on the hydrogel surface to form a lipid membrane around porous PNIPA-co-FA microgels.
Unilamellarity, continuity, and nonleakiness of the lipid bilayer formed upon microgel/liposome mixing were proven for the micrometer-sized, hydrophobically modified, pNIPAM/p(NIPAM-co-AA) core-shell hydrogel spheres [25, 28]. It was also demonstrated by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM)  that hydrophobic modification of the nanogels is not required for spontaneous formation of the bilayer on their surface.
Interesting behavior of the lipobeads was observed when their hydrogel cores change its volume. It was shown (Figure 10) that a collapse of the hydrophobically modified PNIPA microgels within the lipid bilayer caused a shape change of the lipobeads from sphere (below
3.3. Lipobeads by polymerization within liposomal interior
3.3.1. Lipobeads with hydrophobically modified nanogels
As shown by DLS, the size distribution of lipobeads with hydrophobically anchored PNIPA-VI nanogels became bimodal when temperature was raised to 40°C: the position of the first peak corresponded to the initial size of lipobeads at 25°C, while the second peak was assigned to the aggregates of lipobeads. The further cooling back to 25°C restored the original unimodal size distribution of lipobeads, indicating reversibility of anchored lipobeads aggregation. Figure 11 sketches the behavior of lipobeads resulted from the anchored hydrogel collapsing.
Presumably, the aggregation reduces the hydrophobic/hydrophilic imbalance caused by collapsed nanogels within lipobeads. The reversible dissociation of the lipobead aggregates may evidence that anchored lipobeads do not fuse. The only explanation is that the hydrophobic chains of anchored PNIPA-VI nanogels penetrate into the lipid bilayer and stabilize the liposomal membrane against fusion.
On the contrary to the hydrophobically modified giant lipobeads (Figure 10), the anchored PNIPA-VI nanolipobeads did not reveal a size change under temperature or pH variations, as shown by DLS . Probably, on the nanometer scale, a highly curved lipid bilayer is too stiff to follow the collapse of the hydrogel core. This effect remains to be proven.
3.3.2. Lipobeads with nonmodified nanogels
In contrast to the hydrophobically modified lipobeads, the unanchored lipobeads exhibited the unimodal size distribution recorded by DLS at 40°C. The single peak was significantly shifted toward a greater average diameter than that at 25°C. This observation indicated that a more pronounced aggregation occurred in this case. After cooling back to 25°C, the bimodal size distribution of lipobeads was observed. The presence of two peaks indicated that not all aggregates of lipobeads did break up into elementary lipobeads. This pattern of the lipobeads behavior with temperature suggests that the aggregation of lipobeads at elevated temperatures can be irreversible, if lipid bilayers fuse to form a “giant” lipobead containing several nanogels as sketched in Figure 12. Herein, the aggregation of unfused lipobeads is reversible (see processes 1 and 2).
The effect of temperature on shape and size of lipobeads was studied  using the giant (not nano-) nonanchored PNIPA lipobeads prepared by polymerization within giant vesicles. As shown microscopically, the giant lipobeads retained their spherical shape when hydrogel core collapsed at high temperature and swelled back after cooling. Their size was found to change reversibly, so that after six cycles of heating/cooling around the volume transition temperature, the lipobeads remained undamaged. The authors postulated that the membrane was coupled to the gel during the volume change, although the study of mechanisms of the gel core/lipid membrane interactions are still in demand.
4. Special applications and new perspectives
4.1. Lipobead-based drug delivery systems
To figure out which properties make lipobeads attractive for the next generation of drug delivery systems, it is worthwhile to consider first their fate in the body once being administered.
4.1.1. From injection to internalization of lipobeads into the cells
Different administration routes including intravenous, intramuscular, pulmonary, and topical could be suitable to deliver drugs by lipobeads. However, the peripheral intravenous injection seems the most reliable and reproducible route for their administration. Once entering the bloodstream after intravenous injection, lipobeads, similar to liposomal or polymeric delivery systems, should withstand a number of environmental (physiological and physicochemical) attacks on the way to targeted organs. The bloodstream is a complex environment of the serum (proteins, electrolytes, etc.) and immune system (macrophages, proteins of complement system, etc.) components, so that interaction of those components with lipobeads could result in either leakage of their content or their removal from the blood circulation as exogenous pathogens. As it was reported for liposomes, the proteins of complement system were able to produce lytic pores and enhance the release of liposomal content , whereas blood lipoproteins destabilized liposomes to enhance the leakage of their payload . The opsonins and dysopsonins are another blood proteins, which could be responsible for recognition of lipobeads and their enhanced uptake by the mononuclear phagocyte system (MPS
The effect of physicochemical factors (size, charge, hydrophobicity, surface morphology, and composition) on lipobeads’ leakage in and clearance from blood is not known and should be the other target for the future study of lipobeads as a drug delivery system. Nonetheless, even just a few results available on the drug-encapsulated lipobeads (pegylated  or not ) have already demonstrated their noticeably better stability, biodistribution, nontoxicity, and therapeutic activity than those for liposomes.
Once reaching the heart, the blood with lipobeads is pumped up to organs. Undoubtedly, the mechanical stability of lipobeads in the blood flow will be higher than that of liposomes, since in this construct, a lipid bilayer is supported by hydrogel core and can be strengthened even more by anchoring. The capillaries with a diameter ranging from 2 to 10 μm constitute the first sieving constraint for the lipobead size. The particles of the size between 0.4 and 3 μm would mainly be captured by the liver macrophages. The lipobeads greater than 200 nm  would preferentially be filtered by the spleen. The smaller limit comes from the fact that particles less than 40 nm  should undergo clearance through metabolism in the liver and excretion through kidneys. Therefore, the diameter of lipobeads is supposed to be in a relatively narrow range from 50 to 180 nm for a longer retention in the bloodstream. Interestingly, it has been proven that formulations of lipobeads were the most reproducible in this range of sizes especially if prepared by polymerization within a liposomal reactor (see Table 2).
This range of sizes looks appropriate for lipobeads to exit systemic circulation. To reach interstitial space, lipobeads must cross a thin inner membrane of squamous endothelial cells provided by the capillaries. In normal capillaries, the endothelial cells form uninterrupted linings with typical gaps of 5–10 nm in size. In capillaries associated with pathologies such as tumor and inflammation, the gaps between endothelial cells were reported to vary from 100 to 780 nm for different types of cancer . Due to rapid and imbalanced vessel formation, the tumor neovasculature is chaotic, extremely heterogeneous and “leaky” . The enhanced vascular permeability of the tumor capillaries is the first factor contributing to the phenomenon referred as the enhanced permeation and retention (EPR) effect [165, 166]. The second factor of the EPR effect, an enhanced retention of lipobeads in the interstitial space, can be expected due to a poor lymphatic drainage in the tumor tissue, which results in a slower clearance of drug carriers and their accumulation in the interstitial space . Biodistribution experiments have already performed in mice bearing a distant subcutaneous tumor and in mice with metastatic lung melanoma to show accumulation of drug-loaded lipobeads both in the area surrounding the tumor and within the tumor itself . Therein, the payload is evident in the interstitial spaces between the tumor cells outside the vasculature.
In the interstitial space, lipobeads passively or actively target the cellular surface. Strategies of active cell targeting which has been proposed for liposomal carriers [86–88] could be applicable to lipobeads as well.
The internalization of lipobeads into the cells can proceed via several mechanisms [168, 169] sketched in Figure 13. Phagocytosis provides the so-called “cell eating” mechanism by which larger lipobeads can be taken into and degraded within the cells. Using pinocytosis, the cells internalize the fluid surrounding the cell simultaneously with all substances (“cell drinking” mechanism), so that if lipobeads are in the fluid phase area of invagination, they would be taken up to form pinosomes inside. Different endocytic pathways can be distinguished in accord with the specific molecular regulators (not shown in Figure 13), such as the clathrin-mediated endocytosis, dynamin-dependent and dynamin-independent mechanisms, as well as receptor-mediated endocytosis. In addition, the mutual fusion of cell membrane and lipid bilayer of lipobeads  can occur at the cell surface with internalization of just the drug-loaded nanogels. Understanding the cellular entry of lipobeads, their intracellular trafficking, drug release, and therapeutic action mechanisms are the future topics for studies on lipobeadal drug delivery systems.
4.1.2. New mechanisms of drug release
A drug release profile (the amount of drug released into the bloodstream over time) depends on the properties of the drug itself and drug carrier system. Even a few available examples of drug-encapsulated lipobeads showed that the additional element in their structure, the hydrogel core, significantly prolongs the release time for both high molecular weight (e.g., proteins) and small molecule (e.g., doxorubicin) drugs as compared to conventional liposomes and uncoated hydrogel particles. The characteristic time for release of 50% (
Different applications require different release profiles, and bicompartmental structure of lipobeads brings more options to change the concentration profile of a released drug from a steep rise (burst release) and a cyclic variations (pulsatile release) to a gradual increase up to the value within the therapeutic window is reached (sustained or controlled release). Of particular importance is the capability of lipobeads to provide a better-sustained release, which is the most desirable but more difficult mode to achieve and maintain.
Let us consider the novelty the hydrogel core can bring with regard to drug release mechanisms. Undoubtedly, an advanced property of polymer networks is their responsiveness to environmental stimuli. Depending on possible responses of the hydrogel core (swelling, contraction, and degradation), three mechanisms of drug release from lipobeads could be developed in the future.
In the “sponge-like” mechanism (Table 5), hydrogel core initially is in a swollen state. Nevertheless, encapsulated drug molecules release for a prolonged period as compared to conventional liposomes. When the environment changes (temperature, pH, etc.), the polymer network shrinks, so that the hydrogel core, like a squeezed sponge, releases the loaded drug into the space between gel and lipid membrane, and the drug diffuses through the membrane outside the lipobead. This mechanism provides a slow gradual drug release in response to temperature change, for example. The characteristic time of the drug diffusion through the lipid bilayer could be projected to hours.
In the “poration” mechanism, the hydrogel core initially is a shrunken state, and drug molecules are trapped more tightly within the polymer network. Their release can be even more suppressed in comparison with conventional liposomes. When the environment changes (temperature, pH, etc.), the polymer network swells so much that the volume of hydrogel core becomes greater than the space provided by the closed lipid bilayer. Therefore, a “growing” hydrogel core causes stretching of the lipid bilayer and pore formation (“poration”) resulting in the drug release through the pores. This mechanism provides a faster drug release in response to stimuli with the projected characteristic time of minutes.
The “exploding” lipobeads have been discovered  as a byproduct of biodegradation of microgels covered with phospholipid membrane. As schematically outlined in Table 5 (the bottom row), if a polymer network degrades (for example, the interchain cross-links can be cleaved by hydrolysis), the swelling pressure inside increases, because the degradation products are unable to diffuse through the lipid membrane even it stretches. At some point, the internal pressure becomes sufficient to break the membrane. As a result, encapsulated drug falls out of lipobeads with the maximal release rate (“burst” release with the characteristic time of seconds).
4.1.3. Drug combination within lipobeads
In the first example , a combination of protein (Pfs25) and oligonucleotide (CpGODN) has been simultaneously encapsulated into lipobeads. The recombinant protein Pfs25 expressed in
In the second scheme , hydrophilic protein (IL-2, 17 kDa) and hydrophobic small molecule drug (SB505124, SB, 335 Da) have been coencapsulated into the hydrogel core of 120-nm lipobeads cross-linked by a free radical photopolymerization. The IL-2 belongs to the family of cytokines, soluble proteins that supposedly stimulate natural killer cells (NK) and enhance lytic activity against melanomas and renal cancer. However, efficiency of the IL-2 as an immunotherapeutic agent may be significantly reduced by the ability of tumor cells to secret a number of immunosuppressive factors, such as the transforming growth factor-β (TGF-β) that decreases local immune responses. The SB is a TGF-β antagonist that inhibits TGF-β receptor. The study on coencapsulation into lipobeads and simultaneous sustained delivery of the aforementioned drugs showed that no toxicity was observed on intravenously administrated mice. Biodistribution analysis of rhodamine-loaded lipobeads in healthy mice indicated that the lipobeads primarily accumulated in lungs, liver, and kidney, the heart and spleen were also reached though. In B16 lung metastatic animals, the highest accumulation of lipobeads and drug was found to occur in the lungs and liver. In comparison to other delivery systems including liposomal, a significantly greater reduction in both tumor growth rate and tumor mass was observed after one-week therapy of the B16/B6 mouse models of metastatic melanoma administered intravenously. It was found that the lipobead-delivered combination immunotherapy stimulated both innate and adaptive immune systems resulting in drastically increased survival.
4.1.4. Combined multifunctional drug containers
As per Figures 11 and 12, the nanogel core collapse at elevated temperature causes either reversible or irreversible aggregation of lipobeads depending on whether lipid bilayer fusion occurs or not. Reversible and irreversible aggregation of lipobeads is a key step for designing two types of combined multifunctional containers.
In the system made of anchored lipobeads, the initial formulation may consist of two different drugs entrapped in different lipobeads (Figure 14). Under switching condition 1, both drugs can be simultaneously delivered as one aggregate to the targeted organs in the body. At switching condition 2 or 3, either one or the other drug can be released in the desired order.
In the system based on irreversible aggregation of lipobeads (Figure 15), several nanogels loaded with different predrug reagents are trapped under the same lipid membrane (“giant lipobeads”) to react inside without damaging the surrounding organs and to be delivered to the targeted site in one “giant” container able to release the final product controllably.
4.1.5. Remarks on lipobead-encapsulated anticancer drugs
Today, cancer is one of the most dangerous illnesses on Earth, because mortality in patients with solid malignant tumors is caused mainly by tumoral metastases, the appearance of new cancerous centers in another organs or different tissues. Administration of anticancer drugs by intravenous route (chemotherapy) is the main treatment aimed at destruction of primary tumor and reduction of the probability of formation of secondary tumors due to metastases. Since single metastatic cells cannot be localized, followed, and monitored so far, a high concentration of an anticancer drug should be systematically distributed throughout the entire human body to increase the probability of the cancer cells’ distraction. Being strong poisons and/or cancerogenic themselves, anticancer drugs destroy not only malignant cells but also normal ones giving rise to serious side effects of a chronic and irreversible origin and/or causing the formation of a new malignant tumor even without metastases of the primary one. Moreover, the high concentrations of anticancer drugs can induce a resistance of the malignant cells to these drugs. To reduce the toxicity of the anticancer drugs by controlling their suitable concentrations and reaching the targeted cells without healthy cells being affected, numerous polymeric nanoparticles and liposomal drug delivery systems are under development or undergo clinical trials. However, only two polymer conjugates and six liposome-encapsulated anticancer drugs were approved to market as the most clinically successful liposomal anticancer products so far (for details, see  and references therein). Probably, loading drugs into the environmentally responsive hydrogel core covered with the lipid layer is the right way to the chemotherapy with superior tumor response and minimal side effects even at a greater loading concentration.
4.2. Prospective applications and perspectives
4.2.1. Hydrogel/lipid bilayer assembly: Mimicking cell membrane system
Recently developed technologies of using the liposome interior as a microreactor and the concept of lipobeads itself inspire the idea of artificial membrane system with controlled properties. The bottom-up approach to design “liposomes-within-giant vesicle” structures (
By analogy with vesosomes, it would be intriguing to design the so-called
The aforementioned membrane/membrane and hydrogel/membrane structures comprise fusion/fission of LUVs and GUVs and can be served as a model system to study exo- and endocytosis, hydrogel/membrane compatibility, loading ability of the lipid bilayers, polymer networks, and interior of GUVs, and interactions between those assemblages when their surfaces are specifically modified.
Besides all the advantages of conventional lipobeads discussed in this chapter, the multicompartmental structure of vesobeads will provide additional protection against degradation and leakage in bloodstream and greater biocompatibility. Moreover, the structure of a vesobead resembles the structure of a macropinosome (Figure 13) and can provide simultaneous internalization of several lipobeads into the cell interior. Indeed, if an external lipid bilayer of vesobead fuses with the cellular plasma membrane, a bunch of loaded lipobeads are injected into cytoplasm.
The next step of lipobead functionalization is incorporation of proteins into their lipid bilayers or/and loading of the hydrogel core with functional proteins. This type of hydrogel/membrane structures has been already named as
Proteins within lipid bilayer
The first proteo-lipobeads were prepared when transmembrane receptors were reconstituted into the lipid bilayer of lipobeads . It was found that the receptors retained their native-specific binding. Recently, new proteo-lipobeads with the controlled orientation of the membrane protein and enhanced stability have been developed by modifying agarose beads with linkers, binding membrane proteins to the linkers, and surface coverage with phospholipids . The lipobead incorporated cytochrome c oxidase was shown to be functional in terms of antibody binding and proton transport modulation. The proteo-lipobeads, as alternatives of living cells for monitoring properties of membrane proteins and ion transport through ionic channels and transporters, did exhibit a higher stability, capability of uniform orientation, and functional activity of the membrane proteins in comparison with proteo-liposomes and polymersomes . The further interplay between lipids and the lipobead-encapsulated proteins will allow one to remodel the dynamic cell membrane systems  and, despite the increase in complexity of a lipobead structure, will bring about new benefits, such as tiny living cells mimicking mechanisms of drug release regulated by signaling.
If ionic channels (transmembrane proteins) incorporated into the lipid bilayer, it would be necessary to measure ionic transport through the membrane without rupturing the lipid bilayer. For these purposes, one can imagine a hemispherical configuration of the lipobead, which would allow an electrical access to the interior as shown in Figure 18. By the way, this device could be used as a biomimetic sensor and a cell analogue, which functional properties could be modeled and studied by changing the inner compartments of the probe.
126.96.36.199. Proteins inside hydrogel core
As it was mentioned in Section 2.5.1, so far the only test enzymes were loaded into the lipobeads interior to show retention of their enzymatic activity after release from the hydrogel core .
By analogy with the enzyme-containing lipid vesicles (liposomes) , one can predict that in turn the near-future development of enzyme-containing lipobeads will follow the same steps and directions, including the studies on (i) methods for adsorption of a variety of enzymes onto the interior or exterior site of the lipid bilayer and for their encapsulation within hydrogel core, (ii) enzyme encapsulation efficiencies, (iii) potential applications, especially, in the biotechnology (e.g., cheese production) and biomedical fields (e.g., enzyme-replacement therapy or for immunoassays). As in the case of liposomes, the enzyme-containing lipobeads could play a twofold role: enzyme carriers and enzymatic nanoreactors. In the first case, the enzyme molecules are expected to be controllably released from the lipobeads at the target site. In the second case, enzymes trapped inside the hydrogel matrix or interspace between the hydrogel core and lipid bilayer catalyze an enzymatic reaction either upon permeation of a substrate across the bilayer or by stimuli-responsive activation of the enzyme molecules.
Obviously, a deeper understanding and modeling of the catalytic activity of the entrapped enzyme molecules will be in demand. In this context, it is worthy to highlight the key property which hydrogel core brings to the enzyme-containing lipobeads: the activity of the enzyme entrapped into the hydrogel can be affected by the 3D-polymeric network density. For example, the studies on activities of enzymes immobilized into the temperature-sensitive hydrogels explicitly indicated that their activity (e.g., urease , β-galactosidase , α-chymotrypsin ) decreased upon PNIPA hydrogel shrinking at elevated temperatures. Since the volume transition in the temperature-sensitive hydrogels is reversible (see Section 3.1.1 for details), the activities of the aforementioned enzymes were restored with temperature decrease beyond the
There may be another case, when the activity of enzyme immobilized into the hydrogel matrix increases upon a polymer network collapse. In accord with the recently proposed model of electrochemical mechanics of bacterial spores , all bacterial spores have a lipobead-like structure consisting of layered protein network (coat), peptidoglycan cross-linked matrix (cortex) enclosed between two lipid membranes, and the spore core containing the genetic information. Some piece of evidence indicates that the co-called cortex lytic enzymes involved into the spore cortex degradation enhance their activity in response to the collapse of the peptidoglycan cortex where these enzymes are located. Note, in this case, the cortex matrix is a substrate for the enzymes, so that its collapse is equivalent to the increase in the substrate concentration, which in turn is responsible for the rate of the degradation reaction.
5. Closing remarks
The concept of lipobeads has been proposed about 30 years ago and the time has come to explore a combination of lipid bilayer and cross-linked polymer network as a logical step of the development of polymeric and liposomal nanoscopic systems to provide the desired level of functionality, which Nature achieves in living cells. Two general approaches to preparation of lipobeads (polymerization within lipid vesicle interior and mixing of separately prepared lipid vesicles and hydrogel particles) actually are the modified methods for fabrication of liposomes or nanogels. Besides attractive properties from both liposomal and polymeric systems, the mechanical stability of the lipid bilayer and environmental responsiveness of the total structure are the two important properties that hydrogel core brings about to the new construct. Bicompartmental structure of lipobeads predefines their numerous future applications in biotechnology and bioengineering, tissue engineering, sensing and molecular recognition, drug and gene delivery, controlled release, artificial muscles, flow control, and so on.
As a platform for drug delivery systems, lipobeads have already been loaded with chemotherapeutics (doxorubicin, a combination of immunotherapeutic agent and inhibitor of growth factor receptor), malaria vaccine (a combination of stage potential blocker and immunostimulator), and dermatological agent (natural moisturizing factor). In animal experiment, the lipobead-delivered combination chemotherapy demonstrated a drastically increased survival.
Further development of the hydrogel/lipid bilayer assemblies may include vesobeads (e.g., many dispersed lipobeads or their aggregate inside of a giant vesicle, a number of nanogels inside of a double membrane), proteo-lipobeads (lipobeads with functional proteins incorporated into lipid bilayers or/and hydrogel core), enzyme-containing lipobeads (a particular case of proteo-lipobeads with controllable enzymatic activity), and hemi-lipobeads (a cell analogue probe with electrical access to its interior).
This chapter shows that additional technological expenses on production of lipobeads will not be a high cost for the aforementioned advantages of their use in the following Era of the Bioscopic Systems.
Financial support for this work was provided by Pace University (Dyson College of Arts and Sciences, Summer Research and Scholarly Research Funds). I would like to thank the entire cohort of my collaborators and all my students participated in this project. I am also grateful to Dr Irina Gazaryan for many comments, which helped clarify my intent.
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