In order to control microbial resistance against commonly used antibiotics, it is indispensable to develop novel and efficient antimicrobial agents. For this purpose, metallic nanoparticles (mainly inorganic) with their antimicrobial activites represent an effective solution for this global problem. However, synthesis of nanoparticles involves the use of expensive, poisonous and dangerous chemicals responsible for different biological and environmental hazards. This fact increases the necessity of developing environment-friendly procedure by means of green synthesis (using plants) and extra-biological methods (using microbes such as bacteria and fungi). More recently, metallic nanoparticles, derived from fungal sources, have demonstrated their potential not only as a new-generation antimicrobial agents but also as anticancer agents. Therefore, this chapter is aimed to explore the various nanoparticles producing fungi with ultimate objective of elucidating the possible (i) mechanism of biosynthesis of metallic NPs by various fungi and (ii) mode of action of these mycosynthesized NPs on bacterial cell. This chapter would certainly increase our knowledge about interaction of nanoparticles with bacterial cell for their use in health biotechnology.
- antibacterial activities
In the field of nanotechnology and nanomaterial, more specifically, nanoparticles are extremely important because of their unique optical, physicochemical, and biological facets. Though their biological synthesis is still at early stages and many material scientists around the globe are working on the production of nanoparticles from different sources including plants, animals, microbes, and metallic compounds like gold, silver, platinum, etc. Various studies on biosynthesis of nanoparticles have been carried out using a wide array of microorganisms such as algae, bacteria, actinomycetes, fungi, yeasts, and viruses [1, 2]. Among the many possible bioresources, biologically active products from fungi and yeast represent excellent scaffolds for this purpose. Since fungi and yeast are very effective secretors of extracellular enzymes and number of species grow fast and therefore culturing and keeping them in the laboratory is very simple . They are able to produce metal nanoparticles and nanostructure via reducing enzyme extracellularly [3, 4]. Not only the harvesting of extracellularly synthesized nanoparticles from fungi is easy and inexpensive [2, 5], they can also be manipulated by controlling the pH, temperature, substrate concentration (metal ions), and reaction time [6, 7]. Therefore, the biosynthesis could be ideally used for large-scale production of nanoparticles for several industrial applications . Importantly, fungi have also secreted fairly large amount of proteins and secondary metabolites extracellularly, and hence, the fungal biomass could reduce the metal ions more easily leading to the rapid formation of nanoparticles . Because of these advances, the myco-based extracellular synthesis method is often considered as a better resource for higher productivity of nanoparticles .
On the other hand, increasing incidence of microbial resistance to clinically approved classes of antibiotics has emerged in recent years and is a major health problem, requiring to develop novel and effective antimicrobial agents [4, 9–11]. Owing to their antibacterial activities, metallic nanoparticles (mainly silver and gold) represent a novel and an effective solution for overcoming bacterial resistance. In general, nanoparticles are divided into two groups, i.e., organic and inorganic, where the later one being significant biomedical agents . These silver and gold nanoparticles, derived from microbial sources, have demonstrated their potential not only as a new generation antimicrobial  agents against a broad spectrum of Gram-positive and Gram-negative bacteria including multidrug-resistant human pathogens, but also as anticancer agents . They have also significantly enhanced the bactericidal activity when used in combination with standard antibiotics . However, synthesis of nanoparticles involves the use of expensive, poisonous, and dangerous chemicals responsible for different biological and environmental hazards. This fact increases the necessity of developing environment-friendly procedure by means of green synthesis (using plants) and extra biological methods (using microbes such as bacteria and fungi) . Therefore, as a part of our continuing search to identify microorganisms with the potential to synthesize nanoparticles with amazing biological properties, interest has spurred on microbes , especially fungi. Indeed a few fungal species around the globe have been reported to secrete nanoparticles. Therefore, the present chapter is aimed to provide an overview of nanoparticles producing fungi, with the ultimate objective of producing effective antimicrobial agents.
2. Biosynthesis of nanoparticles by fungi
In the last decade, biomineralization has been developed as an emerging and attractive technique for the synthesis of metallic nanoparticles. In which microbial cells are preferably being used for the synthesis of nanosized material involving the oxidation/reduction of metallic ions (like silver, gold, platinum, etc.) giving rise to nanostructures and nanoparticles . This green synthesis of metallic nanoparticles undergo three necessary steps (Figure 1) that are (i) choice of appropriate solvent medium, (ii) choice of appropriate reducing agent (environment friendly), and (iii) stabilizing agents or substances for silver nanoparticles stability . Generally, this reduction is carried out by means of microbial enzymes (Figure 1), present in microbes, and considered to be responsible for synthesis of nanoparticles by those organisms [18, 19].
For this purpose, various fungi have been used as novel and assuring resources for manufacturing of nanoparticles extracellularly as well as intracellularly, such as
2.1. Biosynthesis of nanoparticles by
Several fungal strains belonging to genera
2.2. Biosynthesis of nanoparticles by
Certain filamentous fungi such as
2.3. Biosynthesis of nanoparticles by
Biosynthesis of NPs from species belonging to genera
In another study, AgNP were synthesized using aqueous supernatant of
2.4. Biosynthesis of nanoparticles by other fungi
Various other fungal genera were also exploited for the synthesis of silver nanoparticles. Metallic NPs can be synthesized using fungal biomass and cell-free supernatant. Among eukaryotes, yeast was repeatedly explored for the formation of metallic nanoparticles. Since then, many studies were carried out in order to evaluate the fungal potential as potent producer of silver nanoparticles. For example, cadmium nanoparticles (CdS) were synthesized intracellularly by
However, first example of intracellular mycosynthesis of silver and gold NPs was demonstrated using
3. Application of mycosynthesized nanoparticles in biomedics
Mycosynthesized nanoparticles are of extreme importance because of their unique optical, physicochemical and biological facets. These nanosized particles are continuously being used in different fields including electronics , catalytic processes , optical devices , sensor technology , biological labeling , and may suppress the expression of proteins associated with adenosine triphosphate production , agriculture, pharmacology, and environmental monitoring . More recently they are being used as novel antimicrobial and anticancer agents [61, 74–82]. Antimicrobial mediators can be either synthetic and/or partially modified natural compounds [82, 83]. Type of antimicrobials agent(s) may vary according to targeted pathogens, e.g., antibacterial (for bacteria), antifungal (for fungi), and antiviral (for virus). Similarly, their mechanism of action on microbial cell varies according to the nature of the antimicrobial agents and pathogens. Main targets for antimicrobial compounds could be cell wall (cephalosporins, bacitracin, penicillins, etc.) and protein synthesis, DNA replication (tetracyclines, chloramphenicol, lincosamides, etc.) and metabolism (intermediary) processes (valinomycin, Gramicidin A, etc.), and DNA-synthesis (rifampicin, sulfonamides, quinolones, etc.) as described in Figure 2 . However, abusive use of antimicrobials against various pathogens has evolved into the development of highly adapted and resistant microbes. In addition, these resistant microbes are now being spread globally, challenging the treatment to common infections, and posing significant health threat around the globe. Multidrug-resistant bacteria, such as methicillin-resistant
4. Antibacterial activity of mycosynthesized nanoparticles
Uses of nanoparticles in medicine, particularly in the treatment of bacterial infections, have proved them as effective and novel healing tools against microorganisms. Though for centuries, natural silver has already been considered as one of the most safe and nontoxic inorganic antimicrobial agents . Even silver nanoparticles have also exhibited broad-spectrum antimicrobial properties due to its unique physiochemical and biological characteristics . Recently, silver NPs have shown excellent antimicrobial efficacy against certain bacterial pathogens such as
Silver nanoparticles have shown inhibitory effect on growth in both Gram-positive and Gram-negative bacteria. As described earlier, larger surface area of silver nanoparticles are believed to be key player in interaction with bacterial cell wall and would have resulted in more antibacterial activity. Although mechanism of action of silver NPs on bacterial cell is debatable; however, assumed mechanism include (i) adherence of AgNPs with bacterial cell wall causing membrane lysis, ultimately releasing cellular contents leading to destruction of cell structure and death [89–91], (ii) interaction of Ag ions with free thiol groups (−SH) and disulfide bridges (-S-S-) of cellular enzymes and membrane of bacteria to downregulate or inactivate the cellular protein synthesis by interfering the translation [72, 91, 92], (iii) blocking DNA replication , and (iv) inhibition of signal transduction by dephosphorylation of peptide substrates on tyrosine residues (Figure 2) . Furthermore, inhibitory effect of AgNPs varied for different bacterium, e.g., Gram-positive bacteria were more susceptible to silver nanoparticles as compared to Gram-negative one . The authors have found larger inhibition zone (diameter) in case of
5. Anticancer potential of mycosynthesized nanoparticles
About 10 million people are being diagnosed with cancer annually, with higher mortality rate around the globe . It is generally developed due to disturbance in various physiological processes like cell signaling and apoptosis . In this disease, continuous growth of cancer cell due to resistance to wide range of anticancer agents has become the major challenge to pharmacist while treating the disease [95, 96]. Certain factors such as insufficient drug concentrations approaching tumor cells, intolerable cytotoxicity, nonspecific distribution of anticancer agents, and poor monitoring of drug responses particularly in developing countries has made cancer incurable [97, 98].
Recently, the emerging field of nano-biomedicine has highlighted the possibility of metallic NPs to be used for disease diagnosis and cancer treatment in humans . Among various metallic NPs, silver nanoparticles are considered as novel and promising nanoproducts that can be exploited in the field of nanomedicine due to their unique and distinctive properties. Although antibacterial activity of Ag nanoparticles is well established, however, their anticancer activities have recently been reported against various cancerous cell lines [94, 99], and mechanism of action of AgNPs are still under evaluation and there are many ambiguities that should be addressed in future research.
It is well established that silver nanoparticles when interacting with various biomolecules, such as DNA, proteins, and carbohydrates, may cause cell death due to apoptosis or cytotoxicity probably through reactive oxygen species (ROS) [32, 94]. Various factors influencing cytotoxicity of silver nanoparticles include dosage, contact time, and size of particles. For example, in case of human epidermoid larynx (Hep-2) cell line, cellular damage was dose dependent, causing cell death through ROS [96, 100, 101]. In another study, silver nanoparticles have shown cytotoxic effect through loss of mitochondrial integrity and activation of caspase-cascade exhibiting the apoptotic effect leading to cell death. In a recent study, AgNPs from plant extract, inhibited proliferation of human colon cancer cell line HCT15 while suppressing its cellular growth, inhibiting the G0/G1-phase, interfering DNA synthesis, and ultimately causing cell death through apoptosis . Another important criterion for efficient anticancer drug is its ability to induce apoptosis cancer cell since they generally escape from programed cell death . In recent studies it has been shown that silver nanoparticles possess this property . The authors have treated the tumor-bearing mice (
The use of nanoparticles in the field of medicine is proving to be a novel and promising technique in order to treat various infections. Among various NPs, silver NPs have proved to possess therapeutic alternative that can be exploited in diagnostic and treatment of certain bacterial infections and cancer. To date, various fungi have been reported to biosynthesize the silver nanoparticles. A few among them have been evaluated for the treatment of infection(s) caused by bacterial pathogens providing evidence for their potential role as a new generation antimicrobial agents against a broad spectrum of Gram-positive and Gram-negative bacteria including multidrug-resistant human pathogens. In addition, monodispersed silver NPs can be synthesized by controlling various parameters (such as pH, temperature, etc.) to avoid toxic effects on human cells. Furthermore, biosynthesized silver NPs have successfully exhibited the anticancer activities against different cancer cell lines through inhibiting cell progression (cell proliferation), ROS formation, blockage of DNA synthesis, and apoptosis. However, further research work is required to understand possible mechanism of action of antibacterial and anticancer activities of silver NPs on microbial cell. Various factors (physiochemical and biological) affecting bioavailability, biocompatibility, and cellular toxicity of silver NPs at molecular level should also be addressed in future research that will open new insight for their application alone or in combination with other bioactive agents to control and treat the microbial infections and cancer.