Open access peer-reviewed chapter

Evaluation of Herbicide Efficacy, Injury, and Yield in White Lupin (Lupinus albus L.)

Written By

Anika Folgart, Andrew J. Price, Jessica A. Kelton, Edzard van Santen and Glenn R. Wehtje

Submitted: 06 February 2015 Reviewed: 07 October 2015 Published: 26 November 2015

DOI: 10.5772/61708

From the Edited Volume

Herbicides, Agronomic Crops and Weed Biology

Edited by Andrew Price, Jessica Kelton and Lina Sarunaite

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Abstract

White lupin is of increasing interest in the southeastern United States (US) as a winter legume cover crop or as mid-winter forage for ruminants. White lupins are poor weed competitors during early establishment, making effective weed control necessary; however, only three herbicides are currently registered for use in lupin. An experiment was conducted at two Alabama sites in 2007 and 2008 to evaluate herbicide efficacy provided by ten preemergence (PRE) and nine postemergence (POST) herbicides as well as lupin injury and yield. Overall, PRE applied herbicides, particularly imazethapyr, linuron, and flumioxazin, caused less crop injury than POST herbicides while providing ≥ 86% control of annual bluegrass, corn spurry, heartwing sorrel, henbit, and lesser swinecress six weeks after application. Grass-active herbicides, fluazifop and sethoxydim, provided greater than 95% of annual bluegrass control without causing unrecoverable lupin damage. Imazethapyr applied POST controlled shepherd’s purse (96% to 98%), cutleaf evening-primrose (81% to 96%), and wild radish (71% to 99%) without lupin injury. POST-directed spray applications of glyphosate and flumioxazin provided good weed control of corn spurry (80% to 98%) and winter vetch (71% to 95%) but caused significant crop injury due to drift. In general, grain yields were only reduced with the use of chlorimuron, diclosulam, glyphosate, and thifensulfuron. This research suggests there are several herbicides not currently registered that could be beneficial for use in US lupin production.

Keywords

  • Alternative nitrogen source
  • cover crop
  • weed contro

1. Introduction

Conventional agriculture depends on synthetic nitrogen (N) fertilizers and herbicides for high crop performance [1]. Alternative N sources are available in the form of leguminous crops such as Lupinus spp. White lupin is of major interest in the southeastern US because new cultivars exhibit differential vernalization requirements similar to wheat (Triticum aestivum L.) and can be utilized as mid-winter forage. White lupin has been utilized in the southeastern US as a livestock feed, for human consumption and as a winter cover crop in conservation agriculture [2, 3]. Since its introduction in the 1930s, until the 1950s the US lupin production reached over 1 million ha; however, production declined with the loss of government support, cold-weather damage to seed nurseries, and the increased availability of inorganic fertilizers [3-5].

Lupinus spp. are poor weed competitors during early establishment since canopy development is slow, facilitating light penetration and subsequent weed seed germination and yield loss due to competition. Lupin reaches maximum vegetative growth during flowering when it can successfully compete with newly emerging weeds [6]. Effective weed control is necessary to ensure lupin success under competition with weed species for water, nutrients, and light [6, 7].

Previous research has been conducted to compare the effectiveness of herbicides on weed control and potential for crop injury in lupin. A successful preemergence (PRE) herbicide treatment resulting in no crop damage is pendimethalin alone, or in combination with metribuzin [8, 9]. Pendimethalin use in white lupin provided 100% control of Russian thistle (Salsola tragus L.) and prostrate knotweed (Polygonum aviculare L.) [10]. The use of PRE applied metolachlor and alachlor, primarily in mixes with other herbicides, successfully controlled annual grasses and some broadleaf weed species greater than 90% in spring-type white lupin [11, 12]. Additionally, metolachlor, alone or mixed with linuron, did not cause white lupin injury [13].

Knott [8] found that lupin are especially sensitive to postemergent (POST) herbicides. Fluazifop, as a POST application, provided ≥98% control of wheat (Triticum aestivum L.), triticale (x Triticosecale Wittm ex A. Camus), and annual ryegrass (Lolium multiflorum Lam.) without causing injury to the lupin crop [8, 14]. POST application of imazethapyr provided good weed control but resulted in 15% to 24% crop injury and yield reduction [13]. Similarly, Penner et al. [12] found that the use of imazethapyr, as either PRE or POST, caused crop damage of 35% to 60%. Hashem et al. [15] showed that interrow weed control in narrow-leaf lupin provided by paraquat plus diquat increased yields compared to glyphosate alone, glyphosate plus metrabuzin, and glyphosate followed by paraquat plus diquat.

Currently, only three herbicides are registered for use in lupin: S-metolachlor, carfentrazone-ethyl, and glyphosate [16]. Therefore, the objective of this experiment is to investigate the use of chemical weed management practices in white lupin and evaluate their effect on weed control, crop injury, and lupin grain yield.

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2. Materials and methods

Experimental treatment and design. A two-year experiment was established at two test sites on the E.V. Smith Research and Extension Center of the Alabama Agricultural Experiment Station in October 2007 and 2008, respectively. The experiment was a 2 (year) x 2 (location) x 3 (cultivar) x 4 (block) x 24 (weed control) factorial treatment arrangement. The experiment design was a randomized complete block design (r = 4) nested within each year x location x cultivar combination. The weed control factor had 20 levels: one nontreated control, ten PRE-applied herbicides, and nine POST-applied herbicides (Table 1). The two locations of the experiment were the Field Crops Unit (FCU), near Shorter, AL (32.42 N, 85.88 W) and the Plant Breeding Unit (PBU), Tallassee, AL (32.49 N, 85.89 W). At FCU, the experiment was established on a Compass soil; a coarse-loamy, siliceous, subactive, thermic Plinthic Paleudults with a loamy sand surface structure. At PBU, the experiment was conducted on a Compass Soil: a fine-loamy, mixed, semiactive, thermic Typic Hapludults with a sandy loam surface structure. The three cultivars used in the experiment were AU Homer (a high-alkaloid, indeterminate cover crop type), AU Alpha (a low-alkaloid, indeterminate forage type), and ABL 1082 (a low-alkaloid, determinate grain type experimental cultivar).

Treatment Class Rate Unit
None
S-metolachlor + Linuron PRE 1.12 + 1.12 kg ai ha-1
Metribuzin PRE 0.42 kg ai ha-1
Linuron PRE 1.12 kg ai ha-1
S-metolachlor PRE 1.12 kg ai ha-1
Pendimethalin (0.5 X) PRE 0.84 kg ai ha-1
Pendimethalin (1 X) PRE 1.68 kg ai ha-1
Pendimethalin (2 X) PRE 3.36 kg ai ha-1
Diclosulam PRE 0.026 kg ai ha-1
Flumioxazin PRE 0.071 kg ai ha-1
Imazethapyr PRE 0.071 kg ai ha-1
Thifensulfuron (2007) POST 0.071 kg ai ha-1
Carfentrazone (2008) PDS 46.8 g product ha-1
Fluazifop POST 0.84 kg ai ha-1
Fomesafen POST 0.28 kg ai ha-1
2,4-DB POST 0.28 kg ai ha-1
Chlorimuron (2007) POST 0.052 kg ai ha-1
Clove/Cinnamon Oil (2008) PDS 6.9 L product ha-1
Glyphosate PDS 1.12 kg ai ha-1
Sethoxydim POST 0.28 kg ai ha-1
Flumioxazin PDS 0.071 kg ai ha-1
Imazethapyr POST 0.071 kg ai ha-1

Table 1.

Herbicide treatments, timing, and rates for 2007 and 2008 at the Field Crops Unit and Plant Breeding Unit at E.V. Smith Research Center.

Crop management. Inoculated lupin was seeded in four-row plots with a John Deer 1700 four-row vacuum planter1 with a row spacing of 90 cm at a depth of 1.25 cm in October 2007 and October 2008. Seeding density was 17 seeds m-1. Smooth seedbeds were prepared one to two weeks prior to planting in 2007. In 2008, the cultivars were planted in raised beds prepared by a KMC four-row ripper/bedder2 due to concerns about potential saturated soil conditions at both locations. The plot length was 7.5 m at PBU and 7.5 m and 6 m at FCU in 2007 and 2008, respectively. The PRE herbicide treatments were applied one day after planting in both years. Application of POST herbicides followed 13 (2007) to 16 (2008 due to rainfall) weeks after planting.

Ratings. Weed control ratings were recorded at both locations on a scale from 0% (no weed control) to 100% (complete weed control). The nontreated control was used to estimate the level of control in the treated plots. Two weed control ratings per treatment/plot were taken in each study year. The first rating was taken six weeks after planting and PRE application in both years. The second rating was taken 22 and 26 weeks after planting in 2007/2008 and 2008/2009, respectively.

Crop injury ratings were taken on a scale from 0 (no injury/alive) to 10 (complete injury/dead). The nontreated control was considered to have 0 crop injury. In 2007/2008, crop injury ratings were taken three weeks after planting and PRE application and 15 weeks after planting. In 2008/2009, injury ratings were taken four weeks after planting and PRE application and 18 weeks after planting. In study year 2007/2008, plots at PBU and FCU were harvested on June 17, 2008. In study year 2008/2009, plots at FCU were harvested on June 16, 2009 and at PBU on June 29, 2009 due to differences in attaining maturity. The two center rows of each plot were harvested with a 2-row/10 ft Massey Ferguson plot combine3 to determine grain yield (kg ha-1).

Statistical analysis. We used generalized linear mixed models procedures as implemented in SAS4 PROC GLIMMIX to analyze weed control data. This tool is flexible in the analysis of data with nonnormal distribution and unbalanced designs. Violations of normality and homogeneity of variance issues are often encountered when including a nontreated control treatment or percent control data with a large range. Weed control data were modelled using a binary distribution function or arcsine transformed data. Crop injury data were modelled using arcsine transformed data and then analyzed with a normal distribution function. All treatment factors and their interactions were considered fixed effects except the block factor and its interaction with the various treatment factors. Statistical significance was declared at Dunnett’s P < 0.1.

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3. Results and discussion

Weed control. Over the course of the two-year study, 14 weed species were observed. Not all species were present in all environments; therefore, weed control is presented for only those species that appear at both sites in each year of the study. At the first rating after planting, in both years, the following PRE herbicides provided greater than 90% control of all rated weed species when compared to the nontreated included: S-metolachlor5/linuron6 mixture, metribuzin7, diclosulam8, flumioxazin9, and imazethapyr10 (Table 2). Linuron and S-metolachlor alone provided greater than 90% control in most instances except for henbit (Lamium amplexicaule L.), which was controlled by linuron at 86%, as well as lesser swinecress [Coronopus didymus (L.) Sm.] and heartwing sorrel (Rumex hastatulus Baldw.), which were controlled by S-metolachlor at 86% and 88%, respectively (Table 2). The mixture of S-metolachlor/linuron has been used previously in lupin study, even though linuron is not labeled for use in white lupin production in the southeastern US [17, 18]. In this study, at both early weed and late weed ratings, this mixture provided greater than 70% control of all rated weed species. Pendimethalin11 provided good early season control of all weed species at the 0.5X, 1X, and 2X rate with the exception of lesser swinecress and heartwing sorrel, which were controlled less than 50% by the 0.5X and 1X rates.

Treatment Annual bluegrass Corn spurry Heartwing sorrel Henbit Lesser swinecress
Name Class 2008 2007 2008 2007 2007 2008 2008
None Control 5 35 4 4 22 1 3
S-metolachlor/Linuron PRE 94 99 99 94 99 92 93
Metribuzin PRE 96 99 96 98 97 97 96
Linuron PRE 98 99 99 92 95 86 94
S-metolachlor PRE 95 98 76 88 90 98 86
Pendimethalin (0.5X) PRE 86 98 97 48 97 88 45
Pendimethalin (1X) PRE 89 94 94 46 99 97 41
Pendimethalin (2X) PRE 93 98 98 79 99 98 78
Diclosulam PRE 97 99 95 98 99 98 98
Flumioxazin PRE 97 99 99 99 98 99 99
Imazethapyr PRE 90 98 90 97 93 99 95

Table 2.

Mean weed control ratings for 2007 and 2008 six weeks after lupin planting (prior to postemergence herbicide applications) at the Plant Breeding Unit at E.V. Smith Research Center, Tallassee, AL.a

a All means were significantly different from the control plot using the Dunnett’s test with P <0.1.


In 2007, the second weed rating (22 weeks after planting) conducted after POST herbicide applications revealed PRE applied herbicide weed control to be greater than nontreated controls at both FCU and PBU for each rated weed species except for cutleaf evening-primrose (Oenothera laciniata Hill). At PBU, pendimethalin (0.5X rate) provided only 14% weed control and at FCU, cutleaf evening-primrose control was only 23% with the 1X rate of pendimethalin (Table 3). Less than 50% control was achieved for this weed species with the 2X rate of pendimethalin as well as S-metolachlor. The following POST applied herbicides provided greater than 50% control of all rated weed species included: fluazifop12, chlorimuron13, and imazethapyr. With the exception of black medic (Medicago lupulina L.) and crimson clover (Trifolium incarnatum L.), which were controlled by less than 70% (data not shown), imazethapyr controlled all broadleaf weed species by more than 80%. Ivany and McCully [13] evaluated various herbicides for use in sweet white lupin, they also showed that imazethapyr applied PRE and POST provided good broadleaf weed control (80% to 91%). Sethoxydim14 provided good control for all weed species except for cutleaf evening-primrose, which was less than 50% at both sites. The grass weed species, annual bluegrass (Poa annua L.), was successfully controlled by the POST-applied grass active herbicides sethoxydim and fluazifop which is in agreement with previous research evaluating grass control in lupin [14, 19]. Thifensulfuron15 did not provide greater weed control than the nontreated for cutleaf evening-primrose at FCU (15%) and provided less than 50% control of this species at PBU (31%) as well as corn spurry (Spergula arvensis L.) at FCU (43%) (Table 3). Corn spurry control was also less than 50% for fomesafen16 at both FCU (22%) and PBU (37%) and 2,4-DB at FCU (39%). Glyphosate17 and flumioxazin, which were both POST-directed spray applications, provided good weed control of all rated weeds at both locations (Table 3).

Treatment Annual bluegrass Corn spurry Cutleaf evening-primrose Shepherd's purse Winter vetch
FCU PBU FCU PBU FCU PBU FCU PBU FCU PBU
Name Class Mean* Mean* Mean* Mean* Mean Dunnett’s P-value Mean Dunnett’s P-value Mean* Mean* Mean* Mean*
None Control 0 0 0 0 2 0 3 0 6 0
S-metolachlor/
Linuron
PRE 98 97 98 99 92 <0.0001 95 <0.0001 98 99 97 97
Metribuzin PRE 98 78 96 86 94 <0.0001 91 <0.0001 99 99 89 81
Linuron PRE 96 90 97 99 70 <0.0001 83 <0.0001 98 99 94 72
S-metolachlor PRE 98 93 99 99 45 0.0015 36 0.0007 97 95 96 66
Pendimethalin (0.5X) PRE 98 58 99 99 42 0.0031 14 0.5624 97 90 95 86
Pendimethalin (1X) PRE 98 92 99 99 23 0.1595 28 0.0089 99 96 95 61
Pendimethalin (2X) PRE 99 82 99 99 39 0.0065 48 0.0003 99 99 98 73
Diclosulam PRE 95 68 88 97 96 <0.0001 94 <0.0001 99 99 99 97
Flumioxazin PRE 98 80 99 99 97 <0.0001 95 <0.0001 99 99 83 74
Imazethapyr PRE 97 87 88 97 85 <0.0001 92 <0.0001 89 97 79 60
Thifensulfuron POST 98 64 43 98 15 0.5624 31 0.0005 98 99 98 89
Fluazifop POST 97 99 80 65 57 0.0001 50 <0.0001 98 95 94 57
Fomesafen POST 94 67 22 37 59 <0.0001 70 <0.0001 97 98 93 63
2,4-DB POST 93 76 39 60 98 <0.0001 99 <0.0001 99 90 96 76
Chlorimuron POST 99 65 93 98 98 <0.0001 98 <0.0001 99 99 99 98
Glyphosate PDS 98 89 88 92 69 <0.0001 83 <0.0001 97 99 92 71
Sethoxydim POST 97 96 71 84 45 0.0014 45 0.0002 97 91 93 53
Flumioxazin PDS 95 80 98 93 93 <0.0001 88 <0.0001 98 91 95 79
Imazethapyr POST 97 77 86 88 81 <0.0001 85 <0.0001 98 96 91 65

Table 3.

Mean weed control ratings 22 weeks after lupin planting in 2007 at the Plant Breeding Unit (PBU) and the Field Crops Unit (FCU) at E.V. Smith Research Center, Tallassee, AL.

* Denotes means within location that are all significantly different from control using Dunnett’s test with P-value <0.1.


The second weed control rating in 2008 was conducted 26 weeks after planting at both locations. Due to excessive crop injury in 2007, the POST herbicides thifensulfuron and chlorimuron were replaced with carfentrazone18 and a clove/cinnamon oil19 mixture. When compared to a nontreated, PRE herbicides at both locations provided good weed control for rated weed species with the exception of shepherd’s purse [Capsella bursa-pastoris (L.) Medik.] and cutleaf evening-primrose. At PBU, pendimethalin at the 0.5X rate and full rate did not provide better control of shepherd’s purse than the nontreated (Table 4). Similar results were seen at both PBU and FCU for all rates of pendimethalin in cutleaf evening-primrose control with 10% to 12% control with the 0.5X rate, 6% to 23% control with the 1X rate, and 8% to 18% control with the 2X rate. Control of corn spurry at both locations was also lacking for several POST herbicides including: fluazifop (6% to 7%), fomesafen (14% to 19%), 2,4-DB (5% to 6%), and sethoxydim (7% to 45%) (Table 4). Fluazifop, 2,4-DB, and sethoxydim also did not increase control of shepherd’s purse compared to the nontreated at PBU with 32%, 21%, and 36% control, respectively. The clove/cinnamon oil mixture achieved poor control of shepherd’s purse (29%) and cutleaf evening-primrose (14%) at PBU.

Treatment Corn spurry Cutleaf evening-primrose Shepherd’s purse Wild radish Winter vetch
FCU PBU FCU PBU PBU FCU PBU FCU
Name Class Mean Dunnett’s
P-value
Mean Dunnett’s P-value Mean Dunnett’s P-value Mean Dunnett’s P-value Mean Dunnett’s P-value Mean* Mean* Mean*
None Control 0 0 0 0 0 2 0 0
S-metolachlor/
Linuron
PRE 94 <0.0001 97 <0.0001 77 <0.0001 72 <0.0001 92 <0.0001 98 99 96
Metribuzin PRE 74 <0.0001 85 <0.0001 73 <0.0001 81 <0.0001 93 <0.0001 91 98 67
Linuron PRE 83 <0.0001 93 <0.0001 85 <0.0001 75 <0.0001 98 <0.0001 93 99 76
S-metolachlor PRE 57 <0.0001 63 <0.0001 30 0.0182 12 0.1984 42 0.0651 80 94 85
Pendimethalin (0.5X) PRE 78 <0.0001 96 <0.0001 10 0.5673 12 0.1746 3 0.9999 63 97 46
Pendimethalin (1X) PRE 98 <0.0001 92 <0.0001 23 0.0681 6 0.6044 21 0.3890 93 96 92
Pendimethalin (2X) PRE 91 <0.0001 96 <0.0001 18 0.1434 8 0.4548 64 0.0051 96 99 83
Diclosulam PRE 79 <0.0001 91 <0.0001 85 <0.0001 91 <0.0001 99 <0.0001 98 99 98
Flumioxazin PRE 94 <0.0001 98 <0.0001 94 <0.0001 95 <0.0001 98 <0.0001 96 98 90
Imazethapyr PRE 42 0.0003 97 <0.0001 48 0.0003 96 <0.0001 85 0.0002 95 99 49
Carfentrazone POST 23 0.0292 55 0.0001 62 <0.0001 35 0.0007 69 0.0028 74 96 70
Fluazifop POST 7 0.6489 6 0.8047 66 <0.0001 28 0.0041 32 0.1578 43 90 82
Fomesafen POST 14 0.1718 19 0.1242 41 0.0018 75 <0.0001 94 <0.0001 99 99 77
2,4-DB POST 6 0.7628 5 0.9214 82 <0.0001 96 <0.0001 21 0.3932 63 98 73
Clove/
Cinnamon Oil
POST 26 0.0160 32 0.0124 58 <0.0001 14 0.1984 29 0.2074 99 99 51
Glyphosate PDS 94 <0.0001 98 <0.0001 95 <0.0001 91 <0.0001 96 <0.0001 57 97 94
Sethoxydim POST 7 0.6549 45 0.0010 39 0.0028 25 0.0091 36 0.1083 83 95 54
Flumioxazin PDS 80 <0.0001 81 <0.0001 81 <0.0001 68 <0.0001 60 0.0082 72 97 80
Imazethapyr POST 34 <0.0001 96 <0.0001 82 <0.0001 96 <0.0001 97 <0.0001 71 99 68

Table 4.

Mean weed control ratings 26 weeks after lupin planting in 2008 at the Plant Breeding Unit (PBU) and the Field Crops Unit (FCU) at E.V. Smith Research Center, Tallassee, AL.

* Denotes means within location that are all significantly different from control using Dunnett’s test with P-value <0.1.


Crop injury. Two-way interactions (herbicide–cultivar and location–herbicide) were significant; therefore, injury ratings are presented by location and cultivar. Injury ratings presented here as the mean crop injury was taken after the POST herbicide applications. PRE-applied herbicides in 2007 resulted in no significant increases in crop injury in comparison to nontreated, with a few exceptions. Metribuzin caused increased white lupin injury (4.45) at FCU in cultivar AU Alpha; pendimethalin at the 2X rate resulted in increased injury (3.95) at FCU for the same cultivar (Table 5). Although metribuzin injury was not repeated in 2008 for any cultivar, past research in lupin, as well as soybean has shown variable cultivar tolerance to this herbicide [8, 20]. Diclosulam caused significant injury (6.05 to 9.94) at both locations regardless of cultivar. In 2007, POST herbicide applications, in general, caused greater crop injury than PRE herbicide applications. Thifensulfuron and chlorimuron caused significant lupin damage (9.43 to 10.00), regardless of cultivar or location; therefore, they were discontinued in 2008 (Table 5). Thifensulfuron and chlorimuron were initially included in this study since they are registered for use in soybean; however, research has shown variable phytotoxicity among soybean cultivars for both herbicides [21, 22]. Research conducted by Knott [8] suggests that sulfonylurea herbicides such as metsulfuron cause variable crop injury in white lupin, ranging from limited to severe when applied at the normal field rate. Flumioxazin, as a POST-directed spray, caused significant crop injury at each location for each cultivar (4.50 to 7.84). Significant injury resulted from the use of fomesafen at FCU regardless of cultivar; however, increased injury was not observed with this herbicide at PBU. Glyphosate also resulted in increased lupin injury at FCU for ABL 1082 (6.30) and AU Alpha (5.89) (Table 5). Glyphosate is registered for POST-directed application in lupin in the US [16]; however, herbicide drift can easily cause significant crop injury. This was the most likely cause of lupin injury in our study. Injury from POST flumioxazin applications may also be attributed to drift since PRE applications of this herbicide did not result in increased crop injury in most cases; although, in drier soil conditions, increased phytotoxicity of flumioxazin has been observed in other crops. This could pose a risk for increased lupin damage [23]. Fluazifop (0.50 to 3.81), 2,4-DB (0.06 to 0.75), sethoxydim (0.26 to 2.28), and imazethapyr (0.94 to 4.45) did not result in increased lupin injury over the nontreated (Table 5).

Treatment ABL 1082 AU Alpha AU Homer
FCU PBU FCU PBU FCU PBU
Name Class Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s
P-value
Mean crop injury Dunnett’s P-value
None Control 1.49 0.91 0.21 1.68 0.57 1.06
S-metolachlor/
Linuron
PRE 0.38 0.8758 2.16 0.9490 1.68 0.4758 1.22 1.0000 0.26 1.0000 0.57 1.0000
Metribuzin PRE 1.85 1.0000 1.95 0.9891 4.45* 0.0011 1.68 1.0000 0.06 0.9795 1.22 1.0000
Linuron PRE 0.88 1.0000 2.40 0.8482 0.75 0.9980 0.91 0.9997 0.26 1.0000 1.00 1.0000
S-metolachlor PRE 2.05 1.0000 1.22 1.0000 1.04 0.9355 1.95 1.0000 1.68 0.9422 1.46 1.0000
Pendimethalin (0.5X) PRE 2.32 0.9999 1.22 1.0000 0.38 1.0000 1.72 1.0000 0.26 1.0000 0.75 1.0000
Pendimethalin (1X) PRE 1.99 1.0000 0.53 1.0000 0.57 1.0000 1.46 1.0000 1.22 0.9997 0.38 0.9970
Pendimethalin (2X) PRE 2.88 0.9652 1.46 1.0000 3.95* 0.0042 1.22 1.0000 1.46 0.9891 2.00 0.9980
Diclosulam PRE 9.06* <0.0001 6.05* 0.0011 9.94* <0.0001 4.74 0.2180 8.54* <0.0001 6.79* 0.0002
Flumioxazin PRE 1.56 1.0000 0.26 0.9934 2.86* 0.0555 1.00 1.0000 0.13 0.9989 0.75 1.0000
Imazethapyr PRE 1.65 1.0000 1.46 1.0000 2.08 0.2500 1.35 1.0000 0.38 1.0000 1.68 1.0000
Thifensulfuron POST 10.00* <0.0001 10.00* <0.0001 9.52* <0.0001 9.87* <0.0001 10.00* <0.0001 9.43* <0.0001
Fluazifop POST 3.81 0.5138 1.68 0.9997 0.50 1.0000 2.62 0.9997 2.71 0.3323 1.68 1.0000
Fomesafen POST 8.00* <0.0001 2.40 0.8482 6.78* <0.0001 3.36 0.8909 7.37* <0.0001 2.71 0.8047
2,4-DB POST 0.50 0.9631 0.75 1.0000 0.57 1.0000 0.75 0.9934 0.75 1.0000 0.06 0.6103
Chlorimuron POST 9.94* <0.0001 9.94* <0.0001 9.99* <0.0001 9.62* <0.0001 10.00* <0.0001 9.74* <0.0001
Glyphosate PDS 6.30* 0.0060 2.71 0.6636 5.89* <0.0001 1.42 1.0000 2.91 0.2497 2.18 0.9868
Sethoxydim POST 2.28 1.0000 3.81 0.1551 0.26 1.0000 1.22 1.0000 1.22 0.9997 0.75 1.0000
Flumioxazin PDS 7.29* 0.0003 4.50* 0.0452 7.84* <0.0001 3.70 0.7209 6.01* 0.0002 6.02* 0.0024
Imazethapyr POST 4.45 0.2304 0.94 1.0000 1.06 0.9242 1.46 1.0000 1.12 1.0000 1.00 1.0000

Table 5.

Mean crop injury ratings 15 weeks after planting in 2007 at the Plant Breeding Unit (PBU) and the Field Crops Unit (FCU) at E.V. Smith Research Center, Tallassee, AL.

* Denotes mean crop injury significantly different from control within location using Dunnett’s P-value <0.1.


Crop injury in 2008 resulted in less overall lupin injury than in 2007. PRE applied herbicides did not cause significant injury in comparison to a nontreated at either location for any of the cultivars except for diclosulam (5.26 to 9.00), which caused unacceptable injury, regardless of location or cultivar (Table 6). Diclosulam, which is applied either preplant incorporated (PPI) or PRE, is registered in soybean [Glycine max (L.) Merr.] and peanut (Arachis hypogaea L.) with little injury to either crop [24, 25]. Lupin injury from PRE applications of diclosulam was significant for each cultivar included in the experiment. POST-applied herbicides did not increase crop injury over nontreated except for glyphosate (4.49 to 7.76) and fomesafen in AU Alpha at both locations (3.22 to 3.48) and in AU Homer at PBU (4.00) (Table 6). Crop injury from fomesafen was noted in both years of the study with inconsistent injury for each cultivar. In other crops, such as soybean and dry beans, previous research has reported negligible fomesafen injury regardless of cultivar [26, 27]. In this study, however, it is evident that fomesafen can produce significant injury to lupin.

Treatment AU Alpha AU Homer
FCU PBU FCU PBU FCU PBU
Name Class Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value Mean crop injury Dunnett’s P-value
None Control 0.75 1.72 0.57 1.00 1.90 1.46
S-metolachlor/
Linuron
PRE 1.00 1.0000 3.09 0.8164 0.57 1.0000 0.57 0.9999 1.95 1.0000 1.22 1.0000
Metribuzin PRE 2.40 0.3071 1.72 1.0000 0.26 0.9999 0.57 0.9999 0.53 0.4054 1.00 1.0000
Linuron PRE 0.26 0.9871 1.22 1.0000 0.06 0.8164 0.57 0.9999 0.75 0.7267 1.46 1.0000
S-metolachlor PRE 1.88 0.7463 1.22 1.0000 1.22 0.9871 0.06 0.2778 1.95 1.0000 0.57 0.8730
Pendimethalin (0.5X) PRE 0.53 1.0000 0.94 0.9871 0.38 1.0000 0.57 0.9999 0.06* 0.0120 1.22 1.0000
Pendimethalin (1X) PRE 0.53 1.0000 1.72 1.0000 1.06 0.9994 0.38 0.9716 0.26 0.1031 1.00 1.0000
Pendimethalin (2X) PRE 0.91 1.0000 2.11 1.0000 0.57 1.0000 1.00 1.0000 0.38 0.2178 1.00 1.0000
Diclosulam PRE 8.78* <0.0001 5.26* 0.0080 9.00* <0.0001 9.00* <0.0001 7.60* <0.0001 7.26* <0.0001
Flumioxazin PRE 0.75 1.0000 6.28* 0.0002 0.57 1.0000 2.51 0.5138 0.38 0.2178 1.68 1.0000
Imazethapyr PRE 0.57 1.0000 2.51 0.9986 0.26 0.9999 1.22 1.0000 1.46 1.0000 1.00 1.0000
Carfentrazone PDS 0.94 1.0000 1.22 1.0000 1.46 0.8730 0.57 0.9999 1.12 0.9932 1.72 1.0000
Fluazifop POST 1.72 0.8730 1.46 1.0000 1.46 0.8730 1.22 1.0000 0.75 0.7267 1.22 1.0000
Fomesafen POST 2.66 0.1776 3.22 0.7188 3.22* 0.0155 3.48* 0.0648 2.11 1.0000 4.00* 0.0981
2,4-DB POST 1.00 1.0000 2.40 0.9998 0.38 1.0000 0.26 0.8164 0.06* 0.0120 1.00 1.0000
Clove/Cinnamon Oil PDS 0.57 1.0000 2.40 0.9998 0.06 0.8164 1.00 1.0000 0.75 0.7267 1.22 1.0000
Glyphosate PDS 6.01* <0.0001 6.26* 0.0002 4.49* 0.0001 5.25* 0.0002 3.09 0.9334 7.76* <0.0001
Sethoxydim POST 1.42 0.9932 0.75 0.8730 1.00 0.9999 1.00 1.0000 0.75 0.7267 1.00 1.0000
Flumioxazin PDS 1.22 0.9999 1.22 1.0000 0.38 1.0000 1.22 1.0000 1.72 1.0000 1.72 1.0000
Imazethapyr POST 1.22 0.9999 0.75 0.8730 0.06 0.8164 0.38 0.9716 0.94 0.9331 1.46 1.0000

Table 6.

Mean crop injury ratings 18 weeks after planting in 2008 at the Plant Breeding Unit (PBU) and the Field Crops Unit (FCU) at E.V. Smith Research Center, Tallassee, AL.

* Denotes mean crop injury significantly different from control within location using Dunnett’s P-value <0.1.


Grain yield. Mean grain yields (kg ha-1) were much higher for all three cultivars in 2008 as compared to 2007 (Table 7). The grain type cultivar ABL 1082 yielded highest of the three cultivars in both years. The interaction of treatment and cultivar was statistically significant.

ABL 1082. The nontreated had a mean grain yield of 1337 kg ha-1 in 2007 and of 2074 kg ha-1 in 2008. In both years, none of the PRE herbicides, with the exception of diclosulam, reduced yield. Diclosulam caused yield losses of nearly 950 kg ha-1 in 2007 and 1430 kg ha-1 in 2008 (Table 7). Two POST-applied herbicides, thifensulfuron and chlorimuron, had no measurable yields in 2007. In 2008, glyphosate was the only POST-applied herbicide that caused significant yield losses of 1700 kg ha-1.

Treatment ABL 1082 AU Alpha AU Homer ABL 1082 AU Alpha AU Homer
Name Class Mean Dunnett’s P-value Mean* Mean* Mean Dunnett’s P-value Mean Dunnett’s P-value Mean Dunnett’s P-value
None Control 1337 702 555 2074 1957 1219
S-metolachlor/Linuron PRE 1331 1.0000 734 877 1936 1.0000 1108 0.0011 1262 1.0000
Metribuzin PRE 1174 0.9831 778 551 1612 0.2811 1410 0.1150 1368 0.9315
Linuron PRE 1370 1.0000 700 729 2126 1.0000 1484 0.2526 1359 1.0000
S-metolachlor PRE 1176 0.9855 825 671 1910 0.9998 1426 0.1384 1027 0.5331
Pendimethalin (0.5X) PRE 1353 1.0000 664 740 1937 1.0000 1567 0.5104 1522 0.7994
Pendimethalin (1X) PRE 1256 1.0000 767 617 2025 1.0000 1504 0.3048 1233 1.0000
Pendimethalin (2X) PRE 1294 1.0000 719 585 1907 0.9997 1619 0.7094 1442 0.8990
Diclosulam PRE 391 <0.0001 383 214 648 <0.0001 210 <0.0001 548 0.0667
Flumioxazin PRE 1305 1.0000 594 674 1470 0.0565 1264 0.0159 1217 1.0000
Imazethapyr PRE 1323 1.0000 632 630 1742 0.7320 1460 0.1984 1309 1.0000
Thifensulfuron (2007) POST 0 <0.0001 218 177 ---- ---- ---- ---- ---- ----
Carfentrazone (2008) POST ---- ---- ---- ---- 2081 1.0000 1877 1.0000 1203 1.0000
Fluazifop POST 1094 0.6993 893 536 1889 0.9987 1827 1.0000 1573 0.7980
Fomesafen POST 1167 0.9744 666 666 1738 0.7189 1511 0.3234 1372 0.9390
2,4-DB POST 1216 0.9996 892 783 2180 1.0000 1321 0.0364 1580 0.7716
Chlorimuron (2007) POST 0 <0.0001 0 143 ---- ---- ---- ---- ---- ----
Clove/cinnamon oil (2008) POST ---- ---- ---- ---- 2195 1.0000 1618 0.7065 1347 1.0000
Glyphosate PDS 971 0.1563 673 634 364 <0.0001 735 <0.0001 839 0.3234
Sethoxydim POST 1261 1.0000 706 525 1941 1.0000 1309 0.0309 1313 1.0000
Flumioxazin PDS 1229 0.9999 597 652 1938 1.0000 1350 0.0545 1153 1.0000
Imazethapyr POST 1317 1.0000 557 695 2020 1.0000 1226 0.0087 1433 0.9770

Table 7.

Mean grain yield (kg ha-1) for 2007 and 2008 at E.V. Smith Research Center, Tallassee, AL averaged across location.

* Denotes mean grain yield not significantly different from control within cultivar using Dunnett’s P-value <0.1.


AU Alpha. Mean grain yields of 702 kg ha-1 in 2007 and 1957 kg ha-1 were obtained in the nontreated (Table 7). In 2007, none of the PRE- and POST-applied herbicides reduced yield. However, the POST herbicides, thifensulfuron and chlorimuron, yielded 218 kg ha-1 and 0 kg ha-1, respectively. In 2008, diclosulam, with a mean grain yield of 210 kg ha-1, was the only PRE herbicide that reduced mean grain yield of this cultivar. Similarly, glyphosate, with a mean grain yield 735 kg ha-1, was the only POST herbicide that caused significant yield reduction in 2008.

AU Homer. The nontreated control had a mean grain yield of 555 kg ha-1 in 2007 and 1219 kg ha-1 in 2008 (Table 7). None of the PRE and POST herbicide treatments significantly reduced or increased yield as compared to the control in 2007. In 2008, none of the PRE or POST herbicide applications, with the exception of PRE diclosulam (548 kg ha-1), yielded lower than the nontreated control.

Experiments conducted by Payne et al. [4] in the Pacific Northwest showed a maximum white lupin yield of 2128 kg ha-1, but this yield is not stable. In our study, yield within each cultivar varied greatly between years depending on the treatment. The grain-type cultivar ABL 1082 had the highest mean grain yield, followed by the forage-type cultivar AU Alpha and the cover-crop-type cultivar AU Homer. In this experiment, diclosulam, thifensulfuron, chlorimuron, and glyphosate caused major grain yield losses. AU Homer appears to be the least sensitive to herbicide-induced yield reductions, since neither thifensulfuron nor chlorimuron reduced grain yield. Ivany and McCully [13] stated that POST applications of imazethapyr caused severe crop injury and yield loss in sweet white lupin. The results of this study did not confirm their findings. Neither the PRE nor the POST imazethpyr applications caused significant crop injury or subsequent yield reduction. This could be due, in part, to the use of different cultivars than those used by Ivany and McCully [13].

In general, PRE herbicide applications included in this study, excluding diclosulam, could be used in lupin without posing a significant risk of crop injury. Previous observations by Dittman [28] agree with findings that PRE herbicides may cause less lupin injury than POST herbicide options. Certain POST herbicides, such as thifensulfuron, chlorimuron, and fomesafen, are not viable herbicide options for use in lupin. Other POST options, like fluazifop, 2,4-DB, sethoxydim, and imazethapyr, may offer additional options for weed control in lupin without increasing crop injury.

The results of this experiment show that good weed control can be achieved by using a broad spectrum of herbicides that are currently not registered for use in US lupin production such as imazethapyr, flumioxazin, and linuron. With glyphosate and S-metolachlor, which are registered for use in lupin in the US, good weed control in lupin is possible; however, the use of a limited number of active ingredients can potentially increase resistance development in weed species in these systems. Based on these results, it is necessary to expand the number of registered herbicides for use in US lupin production.

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4. Sources of materials

1 John Deere 1700 four-row vacuum planter, John Deere, Moline, IL.

2 Four-row ripper/bedder, Kelley Manufacturing Co., Tifton, GA.

3Two-row Massey Ferguson plot combine, AGCO Corporation, Duluth, GA.

4Statistical Analysis Systems®, version 9.2, SAS Institute, Inc., Cary, NC.

5 S-metolachlor, Dual Magnum®, Syngenta Crop Protection, Inc., Greensboro, NC.

6 Linuron, Lorox® DF, Tessenderlo Kerley, Inc., Phoenix, AZ.

7 Metribuzin, Sencor®, Bayer CropScience, Research Triangle Park, NC.

8 Diclosulam, Strongarm®, Dow AgroSciences, LLC, Indianapolis, IN.

9 Flumioxazin, Valor®, Valent USA Corporation, Walnut Creek, CA.

10 Imazethapyr, Pursuit®, BASF Corporation, Research Triangle Park, NC.

11 Pendimethalin, Prowl® H2O, BASF Corporation, Research Triangle Park, NC.

12 Fluazifop, Fusilade® DX, Syngenta Crop Protection, Inc., Greensboro, NC.

13 Chlorimuron, DupontTM Classic®, E.I. duPont de Nemours & Company, Wilmington, DE.

14 Sethoxydim, Poast Plus®, BASF Corporation, Research Triangle Park, NC.

15 Thifensulfuron, DupontTM Harmony® SG, E.I. duPont de Nemours & Company, Wilmington, DE.

16 Fomesafen, Reflex®, Syngenta Crop Protection, Inc., Greensboro, NC.

17 Glyphosate, Honcho® Plus, Monsanto Company, St. Louis, MO.

18 Carfentrazone, Aim® EC, FMC Corporation, Philadelphia, PA.

19 Clove/cinnamon oil, Weed ZapTM, JH Biotech, Inc., Ventura, CA.

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Written By

Anika Folgart, Andrew J. Price, Jessica A. Kelton, Edzard van Santen and Glenn R. Wehtje

Submitted: 06 February 2015 Reviewed: 07 October 2015 Published: 26 November 2015