Environmental or growth conditions are constantly influencing the development and maturation of organisms. To ensure precise control of gene expression under these normal as well as stress conditions, organisms developed very accurate molecular regulatory mechanisms at transcriptional and posttranscriptional levels. Key roles of small noncoding RNAs (miRNAs and siRNAs) in regulation of gene expression are no longer astonishing us nor are constantly emerging new classes of non-protein coding RNAs (tRNA-derived small RNAs, snoRNA-derived small RNAs, mRNA-derived small RNAs, and long non-coding RNAs). Although there is evidence of involvement of those various classes of noncoding RNAs in response to stress conditions, in most cases, we are still lacking basic information on their targets and interplay between different regulatory pathways. In this review, we focus on “canonical” and “noncanonical” noncoding RNAs and their involvement in abiotic stress responses.
- Noncoding RNAs
- abiotic stress
Biological organisms exposed to any external circumstances that exert a detrimental influence on them are capable of establishing mechanisms of protection and adaptation. To minimize stress influence, several different strategies can be applied, these are tolerance, resistance, and avoidance or ultimately escape. Because of their sedentary lifestyle, plants are restricted to tolerance, resistance, and avoidance mechanisms only, and thus require short-term strategies to quickly and efficiently readapt their metabolism . Therefore, to ensure their survival under unfavorable conditions plants have established refined adjustments to stresses at all levels (anatomical, morphological, cellular, biochemical, and molecular) . On the other hand, microorganisms, such as industrially important baking yeast, have specific and delicately balanced internal conditions for optimal growth and function. Industrial applications expose yeast to multiple unfavorable environmental conditions, therefore accurate stress response is key for yeast cells. When environmental conditions change abruptly, yeast cells rapidly adjust its internal balance to that required for growth in the new conditions.
The physiological and biochemical changes in plants under stress conditions are a result of alteration in abundance of many transcripts and therefore proteins  pinpointing the role of TGS (transcriptional gene expression) and PTGS (post-transcriptional gene expression) in the adaptation to environmental changes. To achieve precise and timely regulation of stress-responsive gene networks in addition to regulatory DNA sequences and different types of proteins, plants, as well as many other organisms, including yeast, have employed the gene expression regulation by RNA molecules.
For many decades RNA was considered to be a passive intermediate in the flow of information from DNA to protein, apart from other well-known and described functions in translation (tRNAs, rRNAs) or splicing (UsnRNA). In 1998, its new role has been discovered and regulatory capacity of RNA molecules immensely broadened our understanding of gene expression processes. Moreover, new sequencing technologies and high-resolution microarray analysis have revealed genome-wide pervasive transcription in many eukaryotes, generating a large number of RNAs of no coding capacity. Now, nearly 20 years after the first discovery of the RNA interference, we know that small non-coding RNAs not only regulate gene expression on post-transcriptional and transcriptional levels, but can also affect the organization and modification of chromatin. Moreover, they regulate growth and development of organisms, organ development, hormone signalling, and the defense against pathogens [4, 5]. They also function during environmental stress adaptation, providing precise regulation of gene expression.
Herein, we wish to describe the roles of non-protein coding RNA molecules during abiotic stresses in industrially important organisms, such as crops and yeasts. We wish to focus on “canonical” small RNAs, such as miRNAs and siRNAs, but also new classes such as mRNA-derived RNAs, tRNA-derived small RNAs, snoRNA-derived small RNAs (sdRNAs), mRNA-derived small RNAs, and long non-coding RNAs (lncRNAs) (Figure 1).
2. Canonical small RNAs
In plants, small RNAs exhibit unexpected complexity, but two main categories can be distinguished based on their biogenesis and function and these are miRNAs (microRNAs) and siRNAs (short interfering RNAs). Baking yeast lack these two categories of small RNAs since their biogenesis and functions involve RNAi machinery, which is absent in
miRNAs are predominantly 21 nucleotides in length  and they are derived from 70–500 nucleotide long, imperfect, hairpin-like structured primary transcripts called pri-miRNAs by the activity of RNase III dicer-like (DCL) enzyme . DCL1 processes primary miRNA transcript into an miRNA-miRNA* duplex with 2nt overhangs at the 3’ end . With the help from hyponastic leaves (HYL1) and SERRATE proteins miRNA duplex is released and then Hua Enhancer 1 (HEN1) methylates 3’ ends of the duplex and therefore stabilizes it by preventing uridylation and subsequent degradation. HASTY, the plant homolog of exportin 5, transports the miRNA duplex into the cytoplasm [8, 9]. The mature miRNA is loaded into RISC (RNA-induced silencing complex) and guides the translational inhibition or cleavage of target mRNAs through the action of AGO proteins in a sequence-specific manner [8–10].
To date, many stress-responsive miRNAs have been identified and the vast majority of their targets are transcriptional factors or proteins playing important roles not only in developmental and growth processes but also in biotic and abiotic stress responses . There appears to exist common pathways of stress responses based on the induction/reduction of similar small noncoding RNA entities. It has been shown that different stress conditions trigger similar responses in various plant species; but on the other hand, the plant’s response to similar stress condition could trigger the expression of different pools of miRNAs even in different varieties of the same plant species.
Drought and salinity are major external factors influencing crop productivity and seed quality worldwide. Understanding plant’s responses to these stresses is therefore of high importance and one of the main research topics nowadays. Several studies showed differential expression profiles of many genes during water deficit conditions [12, 13]. Recent efforts have also led to the identification of many miRNAs altered in response to drought in many plant species such as cowpea , soybean , or
It is established that somewhere between 6% to 20% of worldwide cultivated arable land is affected by excessive concentrations of salt [24–26]. Some cellular and metabolic processes observed in plants during salt stress conditions (as a result of the limitation of the plant's ability to uptake water) are similar to those observed during drought . Nevertheless, there are many genes and pathways that discriminate these two stress conditions [12, 28]. In salt-tolerant and salt-sensitive
Cold stress is one of the most severe abiotic stresses. The disorders triggered by low temperatures depend on the duration of stress condition and the rate of temperature decrease. Cold stress includes chilling (above 0oC) that inhibits water uptake and freezing (below 0oC) that induces cellular dehydration and thus osmotic stress and hyper accumulation of reactive oxygen species . There are several factors (genes and transcription factors) playing important roles in cold stress acclimatization [10, 34–37]. Post-transcriptional mechanisms of cold acclimation include pre-mRNA processing, mRNA stability, and export from the nucleus. During the last few years, an emerging role of microRNAs in cold stress acclimatization and responses began to unveil. Cold, as well as ABA, dehydration, and salt stress upregulate the expression of miR393, miR397b, and miR402. In accordance with the upregulation of miR393 under cold stress, its target, putative E3 ubiquitin ligase SCF complex F-box protein, appears to be downregulated by cold stress in Arabidopsis . Thus, during cold acclimation, the cleavage of E3 ubiquitin ligase mRNA would lead to diminished proteolysis of its targets (probably positive regulators of cold stress). Moreover, one of the targets of miR393 encodes an F-box protein (At4g03190) [39, 40], which is similar to glucose repression resistance 1 (GRR1), a yeast protein involved in glucose repression. Plants can use sugar status as a signal to modulate growth and development in response to abiotic stresses since it has been observed that sugar metabolism is affected by various abiotic stresses. It has been speculated therefore that miR393 could integrate sugar signaling with cold stress responses . Moreover, in sugarcane, it has been observed that miR319 is differentially expressed during cold treatment for different periods of time, in both shoots and roots . These data are in accordance with previous reports showing that miR319 was upregulated in response to cold stress in Arabidopsis [39, 40]. Genome-wide experiments in
miRNAs also appear to play crucial roles in regulation of nutrient homeostasis. There are 14 mineral elements essential for plant growth and development. Phosphate (Pi) is one of the most prominent ones; it is involved in phosphorylation reactions, synthesis of nucleic acids, membrane lipids, but also in energy delivery and often is a limiting nutrient for plant growth. The phosphate acquisition from soil particles requires the secretion of plant enzymes that release phosphate, then phosphate transport through Pht1 family of transporters (phosphate transporters protein family 1). Phosphate homeostasis in cells is regulated through the action of PHO1 and PHO2 proteins, transcription factors PHR1, WRK75, ZAT6, bHLH32, mYB62, in Arabidopsis and PTF1 in rice and microRNAs. MiR399, upregulated in low-phosphate stressed plants [45–47] targets two genes; a phosphate transporter PHO2 and a putative ubiquitin conjugating enzyme UBC24 transcript [6, 48] have been isolated from the phloem and their level increases in the phloem upon phosphate starvation, suggesting their role as phosphate starvation signals being translocated from shoots to roots where they promote phosphate uptake by downregulating PHO2 [49, 50]. Recently, miR-827-3p was shown to be upregulated in nitrogen and phosphorus deficiency conditions [51, 52]. In rice, it has been shown to target two genes encoding proteins containing SPX and MFS (major facilitator superfamily) domains. SPX-domain proteins are involved in Pi sensing and/or Pi transport ; whereas MFS-domain proteins are membrane proteins functioning in small molecule transport . It has been suggested that SPX-MFS proteins might be involved in intracellular or intercellular Pi transport through regulating the expression of Pi transporter (PT) genes  and/or Pi storage or metabolism . Similarly, in Arabidopsis, it targets the nitrogen/phosphate balance regulator Nitrogen Limitation Adaptation (NLA, AT1g02860) transcripts . Overexpression of miR827 under Pi-starvation conditions causes the downregulation of NLA (together with downregulation of PHO2 through the action of miR399) leading to increased levels of phosphate transporter 1 (PHT1) and elevated activity of Pi uptake at the plasma membrane.
Sulfur (S) is another fundamental macronutrient necessary for proper growth and development. For plants, it is available from the soil in sulfate form, it is taken up by the roots, and distributed within the whole plant by sulfate transporters. It is found in amino acids, peptides and proteins, iron-sulfur clusters, and several co-factors, but also in an oxidized form in polysaccharides, lipids, and modified proteins [58, 59]. Sulfate deprivation induces the accumulation of miR395 that targets ATP sulfurylases (APSs) and sulpfate transporter AST68 [6, 60], thus reducing their levels. In Brassica plants, deprived of sulfur, the level of miR395 increases in the root, stem, and leaf tissues, it is however strongest in the phloem [61, 62].
The regulation of copper (Cu) homeostasis is also managed through the action of microRNAs. This micronutrient is indispensable for photosynthesis and plant protection against reactive oxygen species [63, 64]. Under copper-deficient conditions, upregulation of miR398 leads to the downregulation of cytosol and chloroplastic Cu/Zn superoxidase dismutase transcripts level (CSD1 and CSD2) and copper chaperone for superoxide dismutase CCS1 [65, 67]. Cu/Zn SOD can be, therefore, replaced with FeSOD in chloroplasts . The level of miR398 is elevated in Brassica under copper deprivation in leaf, stem, and root tissue but most importantly in the phloem , such as in the case of sulfur deprivation, which is in accordance with other research suggesting that the phloem has a distinct set of microRNAs from leaves and roots and responds specifically to stress conditions . Several other microRNAs have also been recently linked with copper-deficient conditions (miR397, miR408, and miR857). They are predicted to target Cu-containing proteins (laccases and plantacyanin) .
Under high, heavy metal concentrations, plants are subjected to cation imbalances that lead to alterations in plant physiology and biochemistry. It is not surprising, therefore, that in response to these kind of stresses a set of microRNAs is differentially regulated. It has been observed in rice ,
siRNAs are derived from double-stranded RNAs that result from transcription of inverted-repeat sequences, natural cis-antisense transcript pairs, or by the action of RNA-dependent RNA polymerases (RDRs) that convert single-stranded RNA transcripts (e.g., virus replication) into double-stranded RNAs . They also derive from heterochromatic regions and DNA repeats and mediate the silencing maintenance of the regions from which they originate . These are then cleaved by dicer-like (DCL) enzymes to produce 21-24-nucleotide long species, the size depends on the specific catalytic activity of the respective DCL protein. Similarly to miRNAs, siRNAs are then loaded into Argonaute (AGO) protein-containing RISC that guides target regulation at the transcriptional level via the so called RNA-directed DNA methylation (RdDM) or at post-transcriptional level [8, 26, 74]. Several classes of siRNA can be distinguished, such as trans-acting siRNAs (tasiRNA), heterochromatic RNAs (hc-siRNA), and natural antisense siRNAs (nat-siRNA).
Tasi-RNAs are derived from specific genetic loci called TAS genes through a microRNA-dependent pathway [75, 76]. Cleaved TAS transcripts are converted into dsRNAs by RNA-dependent RNA polymerase 6 (RDR6), which are in turn cleaved into 21-nt siRNAs. Four families of TAS genes have been identified in Arabidopsis. TAS2 siRNAs target PPR mRNAs (pentatricopeptide repeat); TAS3 siRNAs target ARF2, 3 and 4 transcription factors; TAS4 siRNAs decrease the level of MYB transcription factors . TAS1 and TAS2 are recognized by miR173, TAS3 by miR390 and TAS4 by miR828 . TasiRNAs derived from TAS4 have been implicated in Pi deficiency conditions. They are directly involved in the biosynthesis of anthocyanins in response to low Pi . A putative siRNA pta22 from
The usage of high-throughput sequencing methods allowed to identify thousands of genes in convergent overlapping pairs that can generate complementary transcripts in rice and
There has also been a report showing changes in 4 siRNA levels in wheat seedlings under cold, heat, salinity, and dehydration stresses. Two of them (named 002061_0636_3054.1 and 005047_0654_1904.1) were downregulated in heat, salinity, and dehydration. The latter one was also responding to the cold stress by being upregulated. The level of another one (080621_1340_0098.1) was decreased under heat but upregulated under cold stress. The fourth one (997927_0100_2975.1) was not differentiating only under heat stress, but downregulated when plants were treated with salinity, cold, and dehydration conditions .
Heterochromatic 24-nt siRNAs are essential components of RdDM pathway . The pathway has been established to protect plants from invasive nucleic acids, such as transposable elements and repetitive sequences, through DNA methylation and chromatin modifications [86–88]. It has been shown that 21-nt and 24-nt hcsiRNAs are the most abundant class (80%) of all small RNAs in plants [60, 89, 90]. The role of hcsiRNAs in abiotic stress responses is not well understood; although in the 1980s, McClintock has already suggested that all kinds of stresses could potentially reshape a plant genome via transpose activation . Global analysis in plants such as Arabidopsis and rice suggests that the vast majority of transposons are inactive, methylated, and targeted by siRNAs . Recently, few researches suggested that hcsiRNAs could play an important role in the adaptation to stress conditions. In Arabidopsis seedlings subjected to heat stress, ONSEN (copia-type retrotransposon) levels have been shown to increase in mutants affected in RdDM pathway . After the recovery period, the level of ONSEN transcripts decreased gradually suggesting a role for hcsiRNAs. What is interesting is that the insertion of new ONSEN copies has been observed during flower development but before gametogenesis. Surprisingly, ONSEN insertions were also able to confer heat stress responsiveness to nearby genes. Together, all these data suggest that hcsiRNAs can contribute to control of transgenerational retrotransposition triggered by abiotic stresses and can participate in new regulatory networks established to protect plants against stress conditions. The detection of mPing transposition into a rice homologue of flowering time gene-CONSTANS in stressed cultivars supported this intriguing hypothesis. It turns out that stress-activated transposons could positively contribute to genome adaptation to growth in colder climates (reviewed in ). Moreover, stress-mediated induction was shown for Tos17 (rice), Tto1 (tobacco), Tnt1 (tobacco), and BARE-1 (barley) retrotransposons. Low temperature treatment was shown to decrease methylation and increase the excision rate of Tam3 transposon by binding its transposase to GCHCG sites immediately after DNA replication, thus preventing de novo sequence methylation (reviewed in ).
3. Non-canonical small RNAs
In plants, RNAi-dependent as well long ncRNAs-dependent mechanisms constitute the vast majority of stress response mechanisms. However, other non-protein coding RNAs are continuingly being discovered. At the same time,
3.1. tRNA-derived small RNAs
In the expanding repertoire of small noncoding RNAs, tRNA-derived RNA fragments have been identified in all domains of life [reviewed in 94]. According to the nomenclature based on tRNA fragment size and the part of the tRNA molecule from which fragments are derived , tRNA fragments can be separated in two major classes: tRNA halves and smaller tRNA fragments (tRFs). In most of the cases reported till now, full-length tRNA levels do not decline significantly and tRNA fragment levels are consistently lower than those of full-length tRNAs, suggesting that only a small proportion of tRNAs is subjected to cleavage. This is in contrast to the complete depletion of tRNAs targeted by colicins in bacteria . Recent evidences suggest that tRNA-derived fragments are not just a random degradation products but rather stable entities that may have major biological functions. The direct influence on protein biosynthesis has been revealed since: (i) tRF binds to 30S and inhibit translation in
So far, only few reports on plant tRNA-derived fragments are published. Such small RNAs have been observed under non-stress conditions in plants ; however, the basal levels of tRNA halves are low and often increase during stress conditions. In 2009, it has been reported that tRNA fragments are present abundantly under phosphate (Pi) deficiency in
A comprehensive expression profile of non-coding small RNAs was also performed in barley (
Deep sequencing technologies also provided evidence that novel subset of small RNAs are derived from the chloroplast genome (csRNAs) of Chinese cabbage . The chloroplast small RNAs (csRNAs) included, among others, those that derive from tRNAs. Wang et al. found that the csRNAs derived from most of the chloroplast tRNA sequences constituted 5’ parts of the molecules. Moreover, the first nucleotide of these csRNAs were predominantly located at the first nucleotide of the mature chloroplast tRNA sequences, revealing that the biogenesis of csRNA in tRNA molecules was specific to the 5' end. The tRNA-derived csRNAs only slightly declined in the heat-treated seedlings. However, the length of these csRNAs was related to heat stress response. In the heat-treated seedlings, the abundance of longer csRNAs (29–32 nt) decreased, but the of the shorter ones (16–25 nt) increased. csR-trnA-1 and csR-trnA-2 were the two most predominant tRNA-derived csRNA families and originated from chloroplast tRNAAla. Such reduced abundance of tRNA-derived fragments 1 h after the heat treatment (the time period before the appearance of leaf etiolation) may suggest that the heat-responsive csRNAs play roles in the maintenance of subcellular structures and photosynthetic capacity of chloroplasts. The csRNAs derived from tRNAs may, therefore, play a role similar to the reported tRNA halves under various stress conditions.
In 2013, Loss-Morais et al. summarized all existing sequencing data aiming at the characterization of plant tRNA-derived fragments, their accumulation patterns under abiotic and biotic stresses, the identification of their putative targets, as well as possible association with Argonaute (AGO) proteins . They have inspected 34 Arabidopsis deep sequencing libraries, including 25 AGO-IP libraries and found tRFs in the AGO1, 2, 4, and 7 IP libraries. Both, 5' and 3' tRFs were associated with AGO, mirroring previous results in mammalian systems [104–105]. Interestingly, tRFs from the central part of tRNAs were also detected, although 5' tRFs formed the most abundant class and showed the highest sequence diversity. The AGO-associated 5’ tRFs were predominantly 19-mers. Then, to investigate if the 5' tRFs associated with AGOs act in the RNAi pathway in plants, as has been suggested in animals , the authors searched for tRF targets in Arabidopsis and identified four possible target genes. They have found that drought conditions enhanced the expression of the four tRFs, including the 5'-tRFGlyTCC, which was already known to be up-regulated in response to phosphate deprivation .
The existence of transgenerationally transmitted, heat-responsive tRNA-derived fragments in plants was revealed for the first time by Bilichak et al. in
The presence of tRNA-derived fragments has also been reported in industrially important budding yeast
The possible function of tRNA fragments in yeast has been described in 2012 . Although RNA interference (RNAi) is conserved in diverse eukaryotic species, including budding yeast
3.2. snoRNA-derived small RNAs
Small nucleolar RNAs are a highly evolutionarily conserved class of RNAs, which are present throughout the eukaryotes and whose origin lies in the Archaea. There are two classes of snoRNAs (C/D and H/ACA box) that function as ribonucleoprotein (RNP) complexes to guide enzymatic modification of target RNAs at sites determined by RNA:RNA antisense interactions. Generally, C/D box snoRNAs are ~70–120 nucleotides (nt) long and guide the methylation of target RNAs, while H/ACA box snoRNAs are ~100–200 nt long and guide the pseudouridulation of their targets. These RNAs were initially discovered in the nucleolus and thought to exclusively target ribosomal RNAs inside this sub-nuclear compartment. However, numerous snoRNAs do not possess target RNAs—therefore, they are called “orphan snoRNAs”.
Small nucleolar RNAs are localized in the nucleoli and Cajal bodies in eukaryotic cells. Components of the RNA silencing pathway associate with these structures, and two recent reports have revealed that a human and a protozoan snoRNA can be processed into microRNA-like RNAs [112–113]. By systematic analyses of deep-sequencing libraries from diverse eukaryotic organisms, Taft et al. revealed that small RNAs with evolutionary conservation of size and position are derived from the vast majority of snoRNA loci in animals (human, mouse, chicken, fruit fly), Arabidopsis, and fission yeast . These small RNAs derived from snoRNAs (sdRNAs) in Arabidopsis were strongly associated with AGO7 proteins. Arabidopsis Argonaute proteins preferentially load small RNAs with specific 5′ nucleotides and AGO7 is selective for 5′ uracil (U) and 5′ adenine (A). Intriguingly, H/ACA sdRNAs are dominantly 5′A, while C/D box are dominantly 5′U. Therefore, it is likely that sdRNAs play roles in the regulation of gene expression and transcriptional silencing. In particular, the fact that both miRNAs and sdRNAs are 5′U biased strengthens the link between them and suggests that some snoRNAs, including “orphan” snoRNAs whose targets are unknown, may function solely as intermediates in the sdRNA pathway.
However, snoRNA-mediated change in gene expression should be treated not as an example but as an addition to siRNA-based mechanisms. The reason for this statement is that canonical snoRNAs interact with their target RNAs through their 10–20 nt long antisense-box elements. In contrast, snoRNA-derived small RNAs can interact with other sequence elements with their targets, as they form a different ribonucleoprotein complexes.
The presence of snoRNA processing products was experimentally verified by high throughput sequencing, as well as northern blot hybridization in
Despite the differences in possible mechanism of action of sdRNAs, for both plants and yeast, the presence of small regulatory RNAs in the nucleolus allows us to speculate that the nucleolus is involved in the regulation of expression, possibly in response to cellular conditions.
3.3. mRNA-derived RNAs
Small mRNA fragments have been reported in diverse eukaryotes and a portion of them is stress-related [reviewed in 115]. A part of them is annotated as antisense RNAs (asRNAs), overlapping protein coding transcripts on the opposite strand. Some asRNAs were found to repress sense RNAs and lead to inverse expression between asRNAs and their corresponding sense RNAs in respect to growth phases, stress conditions, or environmental changes. Therefore, the corresponding protein-coding transcripts (mRNAs) represent a mixture of cell-cycle factors, chromatin remodellers, and metabolism related proteins. It has been shown recently that about one-fifth of the ORF genes in
Recent tiling assays in plants (mostly in Arabidopsis) also revealed the majority of stress-responsive novel antisense transcripts . Despite a linear correlation between the expression ratios of selected sense and antisense transcripts, biological functions of most antisense RNAs remains unclear. In plants, it has been postulated that antisense transcripts might have the potential to produce endogenous siRNAs (nat-siRNAs) as mentioned in Paragraph 2.2. Recent genome-wide analysis reported an accumulation of sRNAs in their overlapping region, suggesting the occurrence of an RNA interference event . However, the biological processes of generating nat-siRNAs is not completely understood at this time.
The power of mRNA-derived ncRNAs for rapid global translation attenuator in stress was recently demonstrated in
4. Long non-coding RNAs
Long non-coding RNAs (lncRNAs) are a wide group of molecules identified in yeast, plants, and mammals. In general, lncRNAs can be defined as polyadenylated or nonpolyadenylated, more than 200 nt long transcripts with low protein coding potential (coding for less than 100 amino acids). Acceleration in transcriptome research, achieved thanks to the development of high-throughput technologies such as microarrays or next-generation sequencing methods, allow us now to fully appreciate complicated interactions that lead to precise gene expression regulation. It turned out that apart from genes encoding proteins, transposons, genes for housekeeping RNAs (like ribosomal RNAs), intergenic regions, which lie between protein-coding sequences, are also being expressed. Intriguingly, similarly to mRNA, those transcripts, namely lncRNAs, are capped at the 5’ end and many of them are targets of the splicing process . However, in contrast to mRNA, their expression level is very low and they do not have long open reading frames, which are evolutionary conserved . LncRNA also differ from protein-coding transcripts in ribosome occupancy . Large-scale genomics projects, such as Encyclopedia of DNA Elements (ENCODE), proved that lncRNAs are not only transcriptome noise, but indeed transcripts with biological functions. As a result of the ENCODE project, it has been shown that 75% of human genome is transcribed and about 80% of those RNA molecules have some biochemical function . Most of the intergenic regions of Arabidopsis, rice or corn are shown to be transcribed as well as human and constitute a source of lncRNAs that are polyadenylated. Such polyadenylated stable lncRNAs are transcribed by RNA polymerase II and can be divided into four groups based on their genomic origin and relationship with adjacent protein-coding genes:
intergenic lncRNAs (lincRNAs) that are transcribed from sequences between two genes
intronic ncRNAs (incRNAs) that overlap with intronic sequences within another transcript
natural antisense transcripts (NATs) derived from complementary DNA strand of their associated genes
sense lncRNAs overlapping with one or more exon sequences of the transcript on the same strand.
Recently in Arabidopsis and rice [123, 124], another category of lncRNAs has been described — these are nonpolyadenylated transcripts, 50-300 nt in length with low coding potential, but without any sequence similarity to known ncRNAs. This novel group is referred to as intermediate-sized ncRNAs (im-ncRNAs).
Despite the effort of 20 years of investigation , the elusive role of lncRNAs is still not fully described nor understood. Thus far functions of only few such molecules are characterized. We know that lncRNAs are engaged mainly in transcriptional gene expression regulation by acting as scaffolds for transcriptional factors and genetic modifiers, molecular signals, decoys or guides. Moreover lncRNAs can also encode for miRNA and target specific mRNAs for decay or function as miRNA sponges. Most studies has been performed on animal systems, but although research on plants are limited, the emerging picture is that the regulatory functions of plant lncRNAs are similar to animal ones . Till today, almost 40,000 putative lncRNAs have been identified in
Another feature that complicates the retrieval of true lncRNAs is their weak sequence conservation. It is estimated that only from 2% to 5.5% of lncRNAS are conserved in their primary sequence and only some of them may be associated with short conserved elements. Most likely, it is a result of rapid evolution—lncRNAs are frequent targets of positive selection . Some lncRNAs and their target genes can be distinguished by their conserved synteny across species — those lncRNAs play roles in cis-functions . Other lncRNAs may be recognized by conserved secondary structures, which allow them to interact with RNA-binding proteins . Genome-wide analyses carried out so far determined that expression of different groups of lncRNAs is highly tissue-specific and many of them are responsive to biotic and abiotic stress conditions.
In conjunction with the climatic changes, drought is the condition that has been recently extensively studied, and thus many drought-responsive lncRNAs were identified. In
In the best known model plant,
One of best described mechanism of lncRNAs action is lncRNA transcript IPS1 (Induced by Phosphate Starvation 1). IPS1 can interact with miRNA as a competitor and function as miRNA target mimics, which resembles the miRNA sponges from animal systems. Maintaining the phosphate balance is a complicated mechanism in plants, regulated, among others, by miR399 as described in Paragraph 2.1. Low activity of PHO2, ruled by mRNA cleavage mediated by miR399, causes the elevation of phosphate uptake by increasing the expression of two root phosphate transporters. Phosphate starvation also increases the level of IPS1 transcript that has a 23-nt conserved domain, partially complementary to miR399 with 3-nt mismatch overlapping with the miR399-mediated cleavage site. As a non-cleavable product, IPS1 competes with PHO2 and can therefore weaken the miR399-mediated repression of PHO2 . The miRNA sponge strategy is used in the therapy of human diseases and similar processes in plants (target mimic) and can be a very useful tool in plant research as well as in agricultural applications. As mentioned before, in Arabidopsis to date about 20 putative target mimicry events were predicted, which suggest the potential role of this mechanism in other pathways than the maintenance of phosphate homeostasis .
Another model organism,
The rapidly growing human population is constantly stressing agro-ecosystems. In 2007, a FAO (Food and Agriculture Organization of the United Nations) report stated that only about 3.5% of the global agricultural area is not affected by any of the environmental stresses. Since recent advances in biotechnology and molecular biology have dramatically changed our understanding of gene expression regulation and responses of plants to abiotic stresses, we are now armed in new tools that could help us fight various stress conditions and improve the growth and yield of crops and other industrially important species. Our journey in the small RNA world has begun in the past century, in the 90s, with the discovery of RNA interference pathway, followed by siRNAs and their role in DNA methylation and chromatin modifications. Consequently, new and relatively surprising small RNA groups (tRNA-derived small RNAs, snoRNA-derived small RNAs, mRNA-derived small RNAs) have been discovered and slowly but surely their role in cells and in the adaptation to various environmental conditions is being established. Concurrently, thousands of lncRNAs have already been identified and thousands of them are still waiting to be discovered in different organisms, tissues, or in response to different stimuli. With the advent of new high-throughput techniques, it became easier and much faster to collect a vast amount of different types of molecular data. The analysis of such a high amount of data and understanding of mutual correlations and relationships between genes, their roles, and small RNAs is now a challenge that researchers and scientists must face. Nevertheless, we believe that by combining new methodologies with the help of bioinformatic approaches and wet lab experiments, we can shed light on a genomic "dark matter” and start to appreciate important physiological roles of various types of non-protein coding RNAs, as well as their potential applications.
This work was supported by grants from National Science Center Poland: 7690/B/P01/2011/40,DEC-2011/01/D/NZ9/03631, DEC-2011/01/N/NZ9/02900, DEC-2012/06/A/NZ9/00125 and 2014/13/D/NZ1/00061. This publication was also supported by the Polish Ministry of Science and Higher Education, under the KNOW program.
Boyko A, Kovalchuk I. Epigenetic control of plant stress response. Environ Mol Mutagen. 2008;49:61-72.
Grativol C, Hemerly AS, Ferreira PC. Genetic and epigenetic regulation of stress responses in natural plant populations. Biochim Biophys Acta. 2012;1819:176-85. DOI: 10.1016/j.bbagrm.2011.08.010.
Saibo NJ, Lourenço T, Oliveira MM. Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses. Ann Bot. 2009;103:609-23. DOI: 10.1093/aob/mcn227.
Ni FT, Chu LY, Shao HB, Liu ZH. Gene expression and regulation of higher plants under soil water stress. Curr Genomics. 2009;10:269-80. DOI: 10.2174/138920209788488535.
Moazed D. Small RNAs in transcriptional gene silencing and genome defence. Nature. 2009;22;457:413-20. DOI: 10.1038/nature07756.
Jones-Rhoades MW, Bartel DP. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Mol Cell. 2004;14:787-99.
Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP. MicroRNAs in plants. Genes Dev. 2002;16(13):1616-26.
Voinnet O.Origin, biogenesis, and activity of plant microRNAs. Cell. 2009; 136:669-87. DOI: 10.1016/j.cell.2009.01.046
Sunkar R, Jagadeeswaran G. In silico identification of conserved microRNAs in large number of diverse plant species. BMC Plant Biol. 2008;8:37. DOI: 10.1186/1471-2229-8-37.
Lv DK, Bai X, Li Y, Ding XD, Ge Y, Cai H, Ji W, Wu N, Zhu YM. Profiling of cold-stress-responsive miRNAs in rice by microarrays. Gene. 2010;459:39-47. DOI: 10.1016/j.gene.2010.03.011.
Guleria P, Mahajan M, Bhardwaj J, Yadav SK. Plant small RNAs: Biogenesis, mode of action and their roles in abiotic stresses. Genomics Proteomics Bioinformatics. 2011;9:183-99. DOI: 10.1016/S1672-0229(11)60022-3.
Bartels D, Sunkar R. Drought and salt tolerance in plants. Crit Rev Plant Sci. 2005;24:23-58.
Shinozaki K, Yamaguchi-Shinozaki K. Gene networks involved in drought stress response and tolerance. J Exp Bot. 2007;58:221-7.
Barrera-Figueroa BE, Gao L, Diop NN, Wu Z, Ehlers JD, Roberts PA, Close TJ, Zhu JK, Liu R. Identification and comparative analysis of drought-associated microRNAs in two cowpea genotypes. BMC Plant Biol. 2011;11:127. DOI: 10.1186/1471-2229-11-127.
Kulcheski FR, de Oliveira LF, Molina LG, Almerão MP, Rodrigues FA, Marcolino J, Barbosa JF, Stolf-Moreira R, Nepomuceno AL, Marcelino-Guimarães FC, Abdelnoor RV, Nascimento LC, Carazzolle MF, Pereira GA, Margis R. Identification of novel soybean microRNAs involved in abiotic and biotic stresses. BMC Genomics. 2011;12:307. DOI: 10.1186/1471-2164-12-307.
Kantar M, Lucas SJ, Budak H. miRNA expression patterns of Triticum dicoccoides in response to shock drought stress. Planta. 2011;233:471-84. DOI: 10.1007/s00425-010-1309-4.
Wei, L., Zhang, D., Xiang, F. and Zhang, Z. Differentially expressed miRNAs potentially involved in the regulation of defense mechanism to drought stress in maize seedlings. Int. J. Plant Sci. 2009;170:979-989.
Zhou L, Liu Y, Liu Z, Kong D, Duan M, Luo L. Genome-wide identification and analysis of drought-responsive microRNAs in Oryza sativa. J Exp Bot. 2010;61:4157-68. DOI: 10.1093/jxb/erq237.
Zhao B, Liang R, Ge L, Li W, Xiao H, Lin H, Ruan K, Jin Y. Identification of drought-induced microRNAs in rice. Biochem Biophys Res Commun. 2007;354:585-90.
Jian X, Zhang L, Li G, Zhang L, Wang X, Cao X, Fang X, Chen F. Identification of novel stress-regulated microRNAs from Oryza sativa L. Genomics. 2010;95:47-55. DOI: 10.1016/j.ygeno.2009.08.017.
Liu HH, Tian X, Li YJ, Wu CA, Zheng CC. Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. RNA. 2008;14:836-43. DOI: 10.1261/rna.895308.
Arenas-Huertero C, Pérez B, Rabanal F, Blanco-Melo D, De la Rosa C, Estrada-Navarrete G, Sanchez F, Covarrubias AA, Reyes JL. Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress. Plant Mol Biol. 2009;70:385-401. DOI: 10.1007/s11103-009-9480-3.
Trindade I, Capitão C, Dalmay T, Fevereiro MP, Santos DM. miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula. Planta. 2010;231:705-16. DOI: 10.1007/s00425-009-1078-0.
Munns R, Tester M. Mechanisms of salinity tolerance. Annu Rev Plant Biol. 2008;59:651-81. DOI: 10.1146/annurev.arplant.59.032607.092911.
Kruszka K, Pieczynski M, Windels D, Bielewicz D, Jarmolowski A, Szweykowska-Kulinska Z, Vazquez F. Role of microRNAs and other sRNAs of plants in their changing environments. J Plant Physiol. 2012;169:1664-72. DOI: 10.1016/j.jplph.2012.03.009.
Khraiwesh B, Zhu JK, Zhu J. Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants. Biochim Biophys Acta. 2012;1819:137-48. DOI: 10.1016/j.bbagrm.2011.05.001.
Golldack D, Lüking I, Yang O. Plant tolerance to drought and salinity: Stress regulating transcription factors and their functional significance in the cellular transcriptional network. Plant Cell Rep. 2011;30:1383-91. DOI: 10.1007/s00299-011-1068-0.
Ding D, Zhang L, Wang H, Liu Z, Zhang Z, Zheng Y. Differential expression of miRNAs in response to salt stress in maize roots. Ann Bot. 2009;103:29-38. DOI: 10.1093/aob/mcn205.
Zhao B, Ge L, Liang R, Li W, Ruan K, Lin H, Jin Y. Members of miR-169 family are induced by high salinity and transiently inhibit the NF-YA transcription factor. BMC Mol Biol. 2009;10:29. DOI: 10.1186/1471-2199-10-29.
Gao P, Bai X, Yang L, Lv D, Pan X, Li Y, Cai H, Ji W, Chen Q, Zhu Y. osa-MIR393: A salinity- and alkaline stress-related microRNA gene. Mol Biol Rep. 2011;38:237-42. DOI: 10.1007/s11033-010-0100-8.
Stephenson TJ, McIntyre CL, Collet C, Xue GP. Genome-wide identification and expression analysis of the NF-Y family of transcription factors in Triticum aestivum. Plant Mol Biol. 2007;65:77-92.
Macovei A, Tuteja N. microRNAs targeting DEAD-box helicases are involved in salinity stress response in rice (Oryza sativa L.). BMC Plant Biol. 2012;12:183. DOI: 10.1186/1471-2229-12-183.
Chinnusamy V, Zhu J, Zhu JK. Cold stress regulation of gene expression in plants. Trends Plant Sci. 2007;12:444-51.
Stockinger EJ, Gilmour SJ, Thomashow MF. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A. 1997;94:1035-40.
Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell. 1998;10:1391-406.
Chinnusamy V, Ohta M, Kanrar S, Lee BH, Hong X, Agarwal M, Zhu JK. ICE1: A regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. Genes Dev. 2003;17:1043-54.
Fowler S, Thomashow MF. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell. 2002;14:1675-90.
Zimmermann P, Hirsch-Hoffmann M, Hennig L, Gruissem W. GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol. 2004;136:2621-32.
Sunkar R, Girke T, Jain PK, Zhu JK. Cloning and characterization of microRNAs from rice. Plant Cell. 2005;17:1397-411.
Liu HH, Tian X, Li YJ, Wu CA, Zheng CC. Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. RNA. 2008;14:836-43. DOI: 10.1261/rna.895308.
Sunkar R, Chinnusamy V, Zhu J, Zhu JK. Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. Trends Plant Sci. 2007;12:301-9.
Thiebaut F, Rojas CA, Almeida KL, Grativol C, Domiciano GC, Lamb CR, Engler Jde A, Hemerly AS, Ferreira PC. Regulation of miR319 during cold stress in sugarcane. Plant Cell Environ. 2012;35:502-12. DOI: 10.1111/j.1365-3040.2011.02430.x.
Lee H, Yoo SJ, Lee JH, Kim W, Yoo SK, Fitzgerald H, Carrington JC, Ahn JH. Genetic framework for flowering-time regulation by ambient temperature-responsive miRNAs in Arabidopsis. Nucleic Acids Res. 2010;38:3081-93. DOI: 10.1093/nar/gkp1240.
Barakat A, Sriram A, Park J, Zhebentyayeva T, Main D, Abbott A. Genome wide identification of chilling responsive microRNAs in Prunus persica. BMC Genomics. 2012;13:481. DOI: 10.1186/1471-2164-13-481.
Fujii H, Chiou TJ, Lin SI, Aung K, Zhu JK. A miRNA involved in phosphate-starvation response in Arabidopsis. Curr Biol. 2005;15:2038-43.
Bari R, Datt Pant B, Stitt M, Scheible WR. PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants. Plant Physiol. 2006;141:988-99.
Chiou TJ, Aung K, Lin SI, Wu CC, Chiang SF, Su CL. Regulation of phosphate homeostasis by MicroRNA in Arabidopsis. Plant Cell. 2006;18:412-21.
Sunkar R, Zhu JK. Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis. Plant Cell. 2004;16:2001-19.
Aung K, Lin SI, Wu CC, Huang YT, Su CL, Chiou TJ. PHO2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene. Plant Physiol. 2006;141:1000-11.
Bari R, Datt Pant B, Stitt M, Scheible WR. PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants. Plant Physiol. 2006;141:988-99.
Lin WY, Huang TK, Chiou TJ. Nitrogen limitation adaptation, a target of microRNA827, mediates degradation of plasma membrane-localized phosphate transporters to maintain phosphate homeostasis in Arabidopsis. Plant Cell. 2013;25:4061-74. DOI: 10.1105/tpc.113.116012.
Hackenberg M, Shi BJ, Gustafson P, Langridge P. Characterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions. BMC Plant Biol. 2013;13:214. DOI: 10.1186/1471-2229-13-214.
Lenburg ME, O'Shea EK. Signaling phosphate starvation. Trends Biochem Sci. 1996;21:383-7.
Marger MD, Saier MH Jr. A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiport. Trends Biochem Sci. 1993;18:13-20.
Yao ZF, Liang CY, Zhang Q, Chen ZJ, Xiao BX, Tian J, Liao H. SPX1 is an important component in the phosphorus signalling network of common bean regulating root growth and phosphorus homeostasis. J Exp Bot. 2014;65:3299-310. DOI: 10.1093/jxb/eru183.
Lin SI, Santi C, Jobet E, Lacut E, El Kholti N, Karlowski WM, Verdeil JL, Breitler JC, Périn C, Ko SS, Guiderdoni E, Chiou TJ, Echeverria M. Complex regulation of two target genes encoding SPX-MFS proteins by rice miR827 in response to phosphate starvation. Plant Cell Physiol. 2010;51:2119-31. DOI: 10.1093/pcp/pcq170.
Thatcher SR, Burd S, Wright C, Lers A, Green PJ. Differential expression of miRNAs and their target genes in senescing leaves and siliques: insights from deep sequencing of small RNAs and cleaved target RNAs. Plant Cell Environ. 2015;38:188-200. DOI: 10.1111/pce.12393.
Rausch T, Wachter A. Sulfur metabolism: A versatile platform for launching defence operations. Trends Plant Sci. 2005;10:503-9.
Kopriva S. Regulation of sulfate assimilation in Arabidopsis and beyond. Ann Bot. 2006;97(4):479-95.
Jones-Rhoades MW, Bartel DP, Bartel B. MicroRNAS and their regulatory roles in plants. Annu Rev Plant Biol. 2006;57:19-53.
Buhtz A, Pieritz J, Springer F, Kehr J. Phloem small RNAs, nutrient stress responses, and systemic mobility. BMC Plant Biol. 2010;10:64. DOI: 10.1186/1471-2229-10-64.
Buhtz A, Springer F, Chappell L, Baulcombe DC, Kehr J. Identification and characterization of small RNAs from the phloem of Brassica napus. Plant J. 2008;53:739-49.
Burkhead JL, Reynolds KA, Abdel-Ghany SE, Cohu CM, Pilon M. Copper homeostasis. New Phytol. 2009;182:799-816. DOI: 10.1111/j.1469-8137.2009.02846.x.
Allen E, Xie Z, Gustafson AM, Carrington JC. micro-RNA-directed phasing during trans-acting siRNA biogenesis in plants. Cell. 2005;121:207-21.
Yamasaki H, Abdel-Ghany SE, Cohu CM, Kobayashi Y, Shikanai T, Pilon M. Regulation of copper homeostasis by micro-RNA in Arabidopsis. J Biol Chem. 2007;282:16369-78.
Abdel-Ghany SE, Pilon M. MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis. J Biol Chem. 2008;283:15932-45. DOI: 10.1074/jbc.M801406200.
Quinn JM, Merchant S. Two copper-responsive elements associated with the Chlamydomonas Cyc6 gene function as targets for transcriptional activators. Plant Cell. 1995;7:623-8.
Huang SQ, Peng J, Qiu CX, Yang ZM. Heavy metal-regulated new microRNAs from rice. J Inorg Biochem. 2009;103:282-7. DOI: 10.1016/j.jinorgbio.2008.10.019.
Zhou ZS, Huang SQ, Yang ZM. Bioinformatic identification and expression analysis of new microRNAs from Medicago truncatula. Biochem Biophys Res Commun. 2008;374:538-42. DOI: 10.1016/j.bbrc.2008.07.083.
Huang SQ, Xiang AL, Che LL, Chen S, Li H, Song JB, Yang ZM. A set of miRNAs from Brassica napus in response to sulphate deficiency and cadmium stress. Plant Biotechnol J. 2010;8:887-99. DOI: 10.1111/j.1467-7652.2010.00517.x.
Ding YF, Zhu C. The role of microRNAs in copper and cadmium homeostasis. Biochem Biophys Res Commun. 2009;386:6-10. DOI: 10.1016/j.bbrc.2009.05.137.
Xie FL, Huang SQ, Guo K, Xiang AL, Zhu YY, Nie L, Yang ZM. Computational identification of novel microRNAs and targets in Brassica napus. FEBS Lett. 2007;581:1464-74.
Chen X. Small RNAs and their roles in plant development. Annu Rev Cell Dev Biol. 2009;25:21-44. DOI: 10.1146/annurev.cellbio.042308.113417.
Trindade I, Santos D, Dalmay T, Fevereiro P. Facing the Environment: Small RNAs and the Regulation of Gene Expression Under Abiotic Stress in Plants. In: Shanker A, Venkateswarlu B, editors. Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives. Publisher: InTech, 2011. p. 113-136. DOI: 10.5772/1762.
Vazquez F, Vaucheret H, Rajagopalan R, Lepers C, Gasciolli V, Mallory AC, Hilbert JL, Bartel DP, Crété P. Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs. Mol Cell. 2004;16:69-79.
Vazquez F, Legrand S, Windels D. The biosynthetic pathways and biological scopes of plant small RNAs. Trends Plant Sci. 2010;15(6):337-45. DOI: 10.1016/j.tplants.2010.04.001.
Mallory AC, Bouché N. MicroRNA-directed regulation: To cleave or not to cleave. Trends Plant Sci. 2008;13:359-67. DOI: 10.1016/j.tplants.2008.03.007.
Hsieh LC, Lin SI, Shih AC, Chen JW, Lin WY, Tseng CY, Li WH, Chiou TJ. Uncovering small RNA-mediated responses to phosphate deficiency in Arabidopsis by deep sequencing. Plant Physiol. 2009;151:2120-32. DOI: 10.1104/pp.109.147280.
Lu S, Sun YH, Amerson H, Chiang VL. MicroRNAs in loblolly pine (Pinus taeda L.) and their association with fusiform rust gall development. Plant J. 2007;51:1077-98. Epub 2007 Jul 17.
Moldovan D, Spriggs A, Yang J, Pogson BJ, Dennis ES, Wilson IW. Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis. J Exp Bot. 2010;61:165-77. DOI: 10.1093/jxb/erp296.
Wang XJ, Gaasterland T, Chua NH. Genome-wide prediction and identification of cis-natural antisense transcripts in Arabidopsis thaliana. Genome Biol. 2005;6:R30.
Lu C, Jeong DH, Kulkarni K, Pillay M, Nobuta K, German R, Thatcher SR, Maher C, Zhang L, Ware D, Liu B, Cao X, Meyers BC, Green PJ. Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs). Proc Natl Acad Sci U S A. 2008;105:4951-6. DOI: 10.1073/pnas.0708743105.
Borsani O, Zhu J, Verslues PE, Sunkar R, Zhu JK. Endogenous siRNAs derived from a pair of natural cis-antisense transcripts regulate salt tolerance in Arabidopsis. Cell. 2005;123:1279-91.
Yao Y, Ni Z, Peng H, Sun F, Xin M, Sunkar R, Zhu JK, Sun Q. Non-coding small RNAs responsive to abiotic stress in wheat (Triticum aestivum L.). Funct Integr Genomics. 2010;10:187-90. DOI: 10.1007/s10142-010-0163-6.
Zilberman D, Cao X, Jacobsen SE. ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation. Science. 2003;299:716-9.
Matzke MA, Birchler JA. RNAi-mediated pathways in the nucleus. Nat Rev Genet. 2005;6:24-35.
Matzke M, Kanno T, Daxinger L, Huettel B, Matzke AJ. RNA-mediated chromatin-based silencing in plants. Curr Opin Cell Biol. 2009;21:367-76. DOI: 10.1016/j.ceb.2009.01.025.
Law JA, Jacobsen SE. Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet. 2010;11:204-20. DOI: 10.1038/nrg2719.
Chen X. Small RNAs and their roles in plant development. Annu Rev Cell Dev Biol. 2009;25:21-44. DOI: 10.1146/annurev.cellbio.042308.113417.
Ruiz-Ferrer V, Voinnet O. Roles of plant small RNAs in biotic stress responses. Annu Rev Plant Biol. 2009;60:485-510. DOI: 10.1146/annurev.arplant.043008.092111.
McClintock B. The significance of responses of the genome to challenge. Science. 1984; 16;226:792-801.
Lisch D. Epigenetic regulation of transposable elements in plants. Annu Rev Plant Biol. 2009;60:43-66. DOI: 10.1146/annurev.arplant.59.032607.092744.
Ito H, Gaubert H, Bucher E, Mirouze M, Vaillant I, Paszkowski J. An siRNA pathway prevents transgenerational retrotransposition in plants subjected to stress. Nature. 2011;472:115-9. DOI: 10.1038/nature09861.
Gebetsberger J, Polacek N. Slicing tRNAs to boost functional ncRNA diversity. RNA Biol. 2013;10:1798-806. DOI: 10.4161/rna.27177.
Sobala A, Hutvagner G.Transfer RNA-derived fragments: Origins, processing, and functions. Wiley Interdiscip Rev RNA. 2011;2:853-62. DOI: 10.1002/wrna.96.
Masaki H, Ogawa T. The modes of action of colicins E5 and D, and related cytotoxic tRNases. Biochimie. 2002;84:433-8.
Gebetsberger J, Zywicki M, Künzi A, Polacek N. tRNA-derived fragments target the ribosome and function as regulatory non-coding RNA in Haloferax volcanii. Archaea. 2012;2012:260909. DOI: 10.1155/2012/260909.
Ivanov P, Emara MM, Villen J, Gygi SP, Anderson P. Angiogenin-induced tRNA fragments inhibit translation initiation. Mol Cell. 2011;43:613-23. DOI: 10.1016/j.molcel.2011.06.022.
Nowacka M, Strozycki PM, Jackowiak P, Hojka-Osinska A, Szymanski M, Figlerowicz M. Identification of stable, high copy number, medium-sized RNA degradation intermediates that accumulate in plants under non-stress conditions. Plant Mol Biol. 2013;83:191-204. DOI: 10.1007/s11103-013-0079-3.
Zhang S, Sun L, Kragler F. The phloem-delivered RNA pool contains small noncoding RNAs and interferes with translation. Plant Physiol. 2009;150:378-87. DOI: 10.1104/pp.108.134767.
Hackenberg M, Huang PJ, Huang CY, Shi BJ, Gustafson P, Langridge P. A comprehensive expression profile of microRNAs and other classes of non-coding small RNAs in barley under phosphorous-deficient and -sufficient conditions. DNA Res. 2013;20:109-25. DOI: 10.1093/dnares/dss037.
Wang L, Yu X, Wang H, Lu YZ, de Ruiter M, Prins M, He YK. A novel class of heat-responsive small RNAs derived from the chloroplast genome of Chinese cabbage ( Brassica rapa). BMC Genomics. 2011;12:289. DOI: 10.1186/1471-2164-12-289.
Loss-Morais G, Waterhouse PM, Margis R. Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets. Biol Direct. 2013;8:6. DOI: 10.1186/1745-6150-8-6.
Haussecker D, Huang Y, Lau A, Parameswaran P, Fire AZ, Kay MA. Human tRNA-derived small RNAs in the global regulation of RNA silencing. RNA. 2010;16:673-95. DOI: 10.1261/rna.2000810.
Cole C, Sobala A, Lu C, Thatcher SR, Bowman A, Brown JW, Green PJ, Barton GJ, Hutvagner G. Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs. RNA. 2009;15:2147-60. DOI: 10.1261/rna.1738409.
Bilichak A, Ilnytskyy Y, Wóycicki R, Kepeshchuk N, Fogen D, Kovalchuk I. The elucidation of stress memory inheritance in Brassica rapaplants. Front Plant Sci. 2015;6:5. DOI: 10.3389/fpls.2015.00005.
Thompson DM, Lu C, Green PJ, Parker R. tRNA cleavage is a conserved response to oxidative stress in eukaryotes. RNA. 2008;14:2095-103. DOI: 10.1261/rna.1232808.
Zywicki M, Bakowska-Zywicka K, Polacek N. Revealing stable processing products from ribosome-associated small RNAs by deep-sequencing data analysis. Nucleic Acids Res. 2012;40:4013-24. DOI: 10.1093/nar/gks020.
Thompson DM, Parker R. The RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae. J Cell Biol. 2009;185:43-50. DOI: 10.1083/jcb.200811119.
Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP. RNAi in budding yeast. Science. 2009;326:544-50. DOI: 10.1126/science.1176945.
Lee YS, Shibata Y, Malhotra A, Dutta A. A novel class of small RNAs: tRNA-derived RNA fragments (tRFs). Genes Dev. 2009;23:2639-49. DOI: 10.1101/gad.1837609.
Ender C, Krek A, Friedländer MR, Beitzinger M, Weinmann L, Chen W, Pfeffer S, Rajewsky N, Meister G. A human snoRNA with microRNA-like functions. Mol Cell. 2008;32:519-28. DOI: 10.1016/j.molcel.2008.10.017.
Saraiya AA, Wang CC. snoRNA, a novel precursor of microRNA in Giardia lamblia. PLoS Pathog. 2008;4:e1000224. DOI: 10.1371/journal.ppat.1000224.
Taft RJ, Glazov EA, Lassmann T, Hayashizaki Y, Carninci P, Mattick JS. Small RNAs derived from snoRNAs. RNA. 2009;15:1233-40. DOI: 10.1261/rna.1528909.
Tuck AC, Tollervey D. RNA in pieces. Trends Genet. 2011;27:422-32. DOI: 10.1016/j.tig.2011.06.001.
Lin CH, Tsai ZT, Wang D. Role of antisense RNAs in evolution of yeast regulatory complexity. Genomics. 2013;102:484-90. DOI: 10.1016/j.ygeno.2013.10.008.
Matsui A, Ishida J, Morosawa T, Okamoto M, Kim JM, Kurihara Y, Kawashima M, Tanaka M, To TK, Nakaminami K, Kaminuma E, Endo TA, Mochizuki Y, Kawaguchi S, Kobayashi N, Shinozaki K, Toyoda T, Seki M. Arabidopsis tiling array analysis to identify the stress-responsive genes. Methods Mol Biol. 2010;639:141-55. DOI: 10.1007/978-1-60761-702-0_8.
Pircher A, Bakowska-Zywicka K, Schneider L, Zywicki M, Polacek N. An mRNA-derived noncoding RNA targets and regulates the ribosome. Mol Cell. 2014;54:147-55. DOI: 10.1016/j.molcel.2014.02.024.
Guttman M, Amit I, Garber M, French C, Lin MF, Feldser D, Huarte M, Zuk O, Carey BW, Cassady JP, Cabili MN, Jaenisch R, Mikkelsen TS, Jacks T, Hacohen N, Bernstein BE, Kellis M, Regev A, Rinn JL, Lander ES. Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals. Nature. 2009 12;458:223-7. DOI: 10.1038/nature07672.
Zhou P, Silverstein KA, Gao L, Walton JD, Nallu S, Guhlin J, Young ND. Detecting small plant peptides using SPADA (Small Peptide Alignment Discovery Application). BMC Bioinformatics. 2013 20;14:335. DOI: 10.1186/1471-2105-14-335.
Chew GL, Pauli A, Rinn JL, Regev A, Schier AF, Valen E. Ribosome profiling reveals resemblance between long non-coding RNAs and 5' leaders of coding RNAs. Development. 2013;140:2828-34. DOI: 10.1242/dev.098343.
ENCODE Project Consortium. An integrated encyclopedia of DNA elements in the human genome. Nature. 2012;489:57-74. DOI: 10.1038/nature11247.
Liu TT, Zhu D, Chen W, Deng W, He H, He G, Bai B, Qi Y, Chen R, Deng XW. A global identification and analysis of small nucleolar RNAs and possible intermediate-sized non-coding RNAs in Oryza sativa. Mol Plant. 2013;6:830-46. DOI: 10.1093/mp/sss087.
Wang Y, Wang X, Deng W, Fan X, Liu TT, He G, Chen R, Terzaghi W, Zhu D, Deng XW. Genomic features and regulatory roles of intermediate-sized non-coding RNAs in Arabidopsis. Mol Plant. 2014;7:514-27. DOI: 10.1093/mp/sst177.
Brown CJ, Hendrich BD, Rupert JL, Lafrenière RG, Xing Y, Lawrence J, Willard HF. The human XIST gene: Analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus. Cell. 1992;71:527-42.
Liu J, Wang H, Chua NH. Long noncoding RNA transcriptome of plants. Plant Biotechnol J. 2015;13:319-28. DOI: 10.1111/pbi.12336.
Wang H, Chung PJ, Liu J, Jang IC, Kean MJ, Xu J, Chua NH. Genome-wide identification of long noncoding natural antisense transcripts and their responses to light in Arabidopsis. Genome Res. 2014;24:444-53. DOI: 10.1101/gr.165555.113.
Lu T, Zhu C, Lu G, Guo Y, Zhou Y, Zhang Z, Zhao Y, Li W, Lu Y, Tang W, Feng Q, Han B. Strand-specific RNA-seq reveals widespread occurrence of novel cis-natural antisense transcripts in rice. BMC Genomics. 2012 22;13:721.DOI: 10.1186/1471-2164-13-721.
Li L, Eichten SR, Shimizu R, Petsch K, Yeh CT, Wu W, Chettoor AM, Givan SA, Cole RA, Fowler JE, Evans MM, Scanlon MJ, Yu J, Schnable PS, Timmermans MC, Springer NM, Muehlbauer GJ. Genome-wide discovery and characterization of maize long non-coding RNAs. Genome Biol. 2014;15:R40. DOI: 10.1186/gb-2014-15-2-r40.
Wen J, Parker BJ, Weiller GF. In Silico identification and characterization of mRNA-like noncoding transcripts in Medicago truncatula. In Silico Biol. 2007;7:485-505.
Shuai P, Liang D, Tang S, Zhang Z, Ye CY, Su Y, Xia X, Yin W. Genome-wide identification and functional prediction of novel and drought-responsive lincRNAs in Populus trichocarpa. J Exp Bot. 2014;65:4975-83. DOI: 10.1093/jxb/eru256.
Xin M, Wang Y, Yao Y, Song N, Hu Z, Qin D, Xie C, Peng H, Ni Z, Sun Q. Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing. BMC Plant Biol. 2011;11:61. DOI: 10.1186/1471-2229-11-61.
Chen J, Quan M, Zhang D. Genome-wide identification of novel long non-coding RNAs in Populus tomentosa tension wood, opposite wood and normal wood xylem by RNA-seq. Planta. 2015;241:125-43. DOI: 10.1007/s00425-014-2168-1.
Boerner S, McGinnis KM. Computational identification and functional predictions of long noncoding RNA in Zea mays. PLoS One. 2012;7:e43047. DOI: 10.1371/journal.pone.0043047.
Zhang YC, Liao JY, Li ZY, Yu Y, Zhang JP, Li QF, Qu LH, Shu WS, Chen YQ. Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice. Genome Biol. 2014;15:512. DOI: 10.1186/s13059-014-0512-1.
Pang KC, Frith MC, Mattick JS. Rapid evolution of noncoding RNAs: Lack of conservation does not mean lack of function. Trends Genet. 2006;22:1-5. DOI: 10.1016/j.tig.2005.10.003.
Ulitsky I, Shkumatava A, Jan CH, Sive H, Bartel DP. Conserved function of lincRNAs in vertebrate embryonic development despite rapid sequence evolution. Cell. 2011;147:1537-50. DOI: 10.1016/j.cell.2011.11.055.
Qi X, Xie S, Liu Y, Yi F, Yu J. Genome-wide annotation of genes and noncoding RNAs of foxtail millet in response to simulated drought stress by deep sequencing. Plant Mol Biol. 2013;83:459-73. DOI: 10.1007/s11103-013-0104-6.
Zhang W, Han Z, Guo Q, Liu Y, Zheng Y, Wu F, Jin W. Identification of maize long non-coding RNAs responsive to drought stress. PLoS One. 2014;9:e98958. DOI: 10.1371/journal.pone.0098958.
Di C, Yuan J, Wu Y, Li J, Lin H, Hu L, Zhang T, Qi Y, Gerstein MB, Guo Y, Lu ZJ. Characterization of stress-responsive lncRNAs in Arabidopsis thaliana by integrating expression, epigenetic and structural features. Plant J. 2010;1:208-11. DOI: 10.1111/tpj.12679.
Shin H, Shin HS, Chen R, Harrison MJ. Loss of At4 function impacts phosphate distribution between the roots and the shoots during phosphate starvation. Plant J. 2006;45:712-26. DOI: 10.1111/j.1365-313X.2005.02629.x.
Franco-Zorrilla JM, Valli A, Todesco M, Mateos I, Puga MI, Rubio-Somoza I, Leyva A, Weigel D, García JA, Paz-Ares J. Target mimicry provides a new mechanism for regulation of microRNA activity. Nat Genet. 2007;39:1033-7. DOI:10.1038/ng2079.
Ben Amor B, Wirth S, Merchan F, Laporte P, d'Aubenton-Carafa Y, Hirsch J, Maizel A, Mallory A, Lucas A, Deragon JM, Vaucheret H, Thermes C, Crespi M. Novel long non-protein coding RNAs involved in Arabidopsis differentiation and stress responses. Genome Res. 2009;19:57-69. DOI: 10.1101/gr.080275.108.
Heo JB, Sung S. Vernalization-mediated epigenetic silencing by a long intronic noncoding RNA. Science. 2011;331:76-9. DOI: 10.1126/science.1197349.
Ding J, Lu Q, Ouyang Y, Mao H, Zhang P, Yao J, Xu C, Li X, Xiao J, Zhang Q. A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice. Proc Natl Acad Sci U S A. 2012;109:2654-9. DOI: 10.1073/pnas.1121374109.
Wu HJ, Wang ZM, Wang M, Wang XJ. Widespread long noncoding RNAs as endogenous target mimics for microRNAs in plants. Plant Physiol. 2013;161:1875-84. DOI: 10.1104/pp.113.215962.
Wang S, Tran EJ. Unexpected functions of lncRNAs in gene regulation. Commun Integr Biol. 2013;6:e27610. DOI: 10.4161/cib.27610.
Solé C, Nadal-Ribelles M, de Nadal E, Posas F. A novel role for lncRNAs in cell cycle control during stress adaptation. Curr Genet. 2014 Sep 28. DOI: 10.1007/s00294-014-0453-y.
Oda A, Takemata N, Hirata Y, Miyoshi T, Suzuki Y, Sugano S, Ohta K. Dynamic transition of transcription and chromatin landscape during fission yeast adaptation to glucose starvation. Genes Cells. 2015;20:392-407. DOI: 10.1111/gtc.12229.