The Maltese medicinal plants in this study.
1. Introduction
In spite of its small size (31,500 hectares), the Maltese Archipelago hosts a large number of medicinal and aromatic plants that have been utilised medicinally for several centuries. The Maltese Archipelago lies in the middle of the Mediterranean Sea, 35°50’ north of the Equator and 14°35’ east of Greenwich. The climate is characterized by hot dry summers, mild wet winters (an average rainfall of 500 mm and temperatures ranging between 13°C in winter and 35°C in summer) and a high relative humidity all the year round. Most of the wild plants thrive in very shallow soil pockets that, in some cases, contribute to the production of phytochemicals as a means of protection against other plants or other organisms. In general, Maltese soils contain a high amount of calcium carbonate (>53%), which is the parent rock material, a high pH (>8) and a high clay content with a good physical structure but lacking organic matter (<4.5 %).
The Maltese flora comprises around 1284 vascular plants 66% originating from the Mediterranean region while the other 34% originating from the cold European and warm subtropical regions [1]. Out of these, there are about 458 medicinal taxa with approximately 300 originating from the Mediterranean region. The main plant families of medicinal importance are Asteraceae (15%), Lamiaceae (7%), Fabaceae (6%), Umbelliferae (4%) and Rosaceae (4%) amongst others. The biodiversity in medicinal flora is high probably due to several reasons that include:
favourable Mediterranean climate
availability of fertile calcareous soils
considerable area of uncultivated land (wastelands)
former conquerors of the Maltese Islands
Maltese interest in herbal medicine
The number of medicinal species is on the decline to the extent that some have already become extinct. This is not mainly attributed to overuse problems but due to various human activities. There were isolated cases where a medicinal plant was under treat due to over-harvesting. One typical example was the seaside squill (
2. Medicinal flora of the Maltese islands
The pharmacological assessment of the Maltese medicinal flora, contributed to a portion of the research conducted on these species. Intensive research has been conducted in other fields, particularly in the ethnobotanical, agronomic,
Medicinal plants have been classified either on their phytochemical constitution or else on their pharmacological activities. These plant contain a myriad of metabolite classes and single metabolites. In most cases, more has to be discovered as the information is either unavailable or else still uninvestigated yet. Locally, medicinal plants have been classified on their pharmacological activity. Some would include the following effects: cardiotonic (e.g. squill, oleander), anticancer (e.g. squirting cucumber, borage), immunomodulatory (e.g. squirting cucumber, olive tree), antiflammatory and skin disorders (e.g. marigold, aloe, erica), antihypertensive (e.g. hawthorn), antimicrobial and antifungal (poison ivy, sage, garden basil, sticky fleabane, couch grass, garlic, fig tree, caper plant, pellitory of the Wall), antidiabetic (karela), insect repellents and insecticides (pennyroyal, tree tobacco), antihelmintic (pumpkin), spasmodic and antispasmodic (vervain, henbane), sedative (blue passion flower, orange-flower water, chamomile), kidney stone problems (micromeria), volatile oil (lavander, garden rue, lemon balm, rosemary, laurel, spearmint) and fixed oils (olive tree, castor oil plant). Some of these plants are listed in table 1.
Local ethnobotanical research has contributed towards the discovery of new leads. In such studies, the traditional claims are challenged using scientific methods. Possible conservation strategies were also considered, particularly for endangered species. However, there are limitations since there are no national incentives to conserve these plant species unless cultivated or sold as pot plants. However, there are few plants that are legally bound. A typical example is the carob tree. The grower cannot uproot a carob tree to pursue cultivation needs.
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Asparagaceae | Seaside squill | Basla tal-għansar |
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Cucurbitaceae | Squirting cucumber | Faqqus il-ħmir |
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Lamiaceae | Pennyroyal | Plejju |
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Lamiaceae | Garden sage | Salvja |
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Verbenaceae | Vervain | Buqexrem |
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Araliaceae | Common ivy | Liedna |
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Rosaceae | Common hawthorn | Anżalor salvaġġ |
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Asteraceae | Pot marigold | Suffejra |
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Lamiaceae | Lemon balm | Burieħa |
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Oleaceae | Olive tree | Żebbuġa |
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Urticaceae | Stinging nettle | Ħurrieqa |
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Capparaceae | Caper plant | Kappara |
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Ephedraceae | Mormon tea | Efedra |
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Solanaceae | Tree tobacco | Tabakk tas-swar |
2.1. Drimia maritima (L.) Stearn
The main constituents of the seaside squill are the cardiac glycosides and phenolic compounds [4]. It also contains mucilage and calcium oxalate crystals. The squill cardiac glycosides are bufadienolides. In principle, these are similar to triterpenoids having a sugar group and a lactone ring at C17. Scillaren A accounts for about 70% of the total glycosidal content of squill. It contains one unit of rhamnose and one unit of glucose. When scillaren A is hydrolyzed by enzymes, it breaks down to proscillaridin A and D-glucose (Figure 2).
These glycosides act by binding to the Na+/K+ ATPase pumps. This occurs due to the presence of the lactone group [5, 6]. These bufadienolides are therefore important cardiotonic, blood pressure stimulating and antitumour agents. The main glycosides with digoxin-like effects are scillaren A and proscillaridin A [7].
Several cultivation parameters were studied for
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First | Control | 0.25 |
Fertiliser-treated | 0.26 | |
Second | Control | 0.66 |
Fertiliser-treated | 0.68 | |
Third | Control | 0.57 |
Fertiliser-treated | 0.58 | |
Fourth | Control | 0.58 |
Fertiliser-treated | 0.58 | |
Fifth | Control | 0.38 |
Fertiliser-treated | 0.40 | |
Sixth | Control | 0.31 |
Fertiliser-treated | 0.34 |
Micropropagation of squill was carried out by direct and indirect organogenesis. Regeneration was successfully achieved using bulb explants by direct organogenesis. Although the process of regeneration was slow, callus cultures maintained in high auxin concentrations (4 mg/l 2,4-D + 2 mg/l NAA) induced root formation, when the plant growth regulators (PGRs) were removed [9].
2.2. Ecballium elaterium (L.) A.Rich.
The main constituents of this plant are the cucurbitacins (Cu), the major ones being CuE and CuB (Figure 4), particularly present in the fruit juice. Other cucurbitacins include cucurbitacins D, G, H, I, R, L, hexanorcucurbitacin I, 16-deoxy-∆16-hexanorcucurbitacin O, anhydro-22-deoxo-3-epi-isocucurbitacin D, and their glycosides [12-14]. The squirting cucumber also contains sterols, fatty acids, elaterases, tannins [15], complex phenolic compounds and flavonoids [16], amino acids and their derivatives as well as the
Although this plant is abundant in wastelands throughout the Maltese Archipelago, micropropagation was attempted for two main reasons. These were as a means to study the responses of explants from the squirting cucumber to different plant growth regulators, and to determine the potential propagation of high-yielding mother plants. In this attempt, seeds were germinated in Murashige-Skoog (MS) medium. Different concentrations and types of PGRs, mainly auxins and cytokinins, were added. Subculturing with the different PGRs was performed every 4 weeks and explants were maintained at about 25 ± 1 °C and 3250 ± 250 lx. Once developed, the plantlets were transferred to Jiffy® pots until rooting and then repotted (compost:peat:perlite, 2:2:1) until flowering [19]. The main four responses of explants were bud multiplication, shoot elongation, callus production and rooting, as illustrated in Figure 5.
A regeneration protocol was devised as follows. Briefly, the seeds were germinated on MS medium (8 - 9 weeks). Bud multiplication of node explants was performed on naphthaleneacetic acid/6-benzylaminopurine (NAA/BAP) medium (for 2 - 3 subcultures every 4 weeks). shoot elongation was obtained on Gibberellic Acid (GA3) medium (4 weeks), followed by an auxin shock on Indole-3-acetic acid (IAA) medium (1 week) then, treated with rooting hormone powder and finally transfer to Jiffy® pots (3 - 4 weeks). The plants were then repotting and acclimatised for 4 - 5 weeks [19]. The whole process takes between 24 and 35 weeks.
The
The production of cucurbitacins from cultivated sources, is significantly higher in fruit compared to stems and leaves (figure 7). A drop in ambient temperature results in lower production of cucurbitacins [21].
Pharmacological testing has been extensively carried out on this plant. Extracts exhibited a marked effect on prostate cancer cells (IC50= 9.35 nM) and moderate effects on melanoma and breast cancer cells (IC50 = 0.87 and 1.95 μM, respectively)
2.3. Mentha pulegium L.
In a study by [27], pennyroyal oil contained 38.0% piperitone, 33.0% piperitenone, 4.7% α-terpineol and 2.3% pulegone as the major components (Figure 8). The authors concluded that Iranian pennyroyal oil is rich in piperitone/piperitenone. In another study, the pulegone content of Iranian pennyroyal oil ranged between 1.3 – 52.0%, when extracted by supercritical fluid extraction, while hydrodistillation yielded around 37.8% of pulegone. Piperitenone consituted only 6.8% to the extracted essential oil [28]. Similarly, in another study [29], the content of pulegone in Greek pennyroyal oil was in the range of 42.9% and 90.7% attributed to two populations. In other wild populations, the pulegone content did not exceed 35.6%. Such populations were rich in either menthone/isomenthone or in piperitone/piperitenone. In Tunisian pennyroyal oil, 41.8 % of the oil was pulegone [30] while Portuguese pennyroyal oil contained 23.2 % of pulegone [31]. The pennyroyal oil was extracted from wild Maltese populations using hydrodistillation with a yield of 0.73 % [32]. The pulegone content in the oil was 85.8 %, followed by other constituents; (-) limonene (0.984 %), myrcene (0.109 %) and β-pinene (0.191 %). This was determined by GC-FID.
Apart from its abortifacient activity, pennyroyal oil is also hepatotoxic and causes pulmonary necrosis. Hepatotoxicity is mainly attributed to the conversion of pulegone into its epoxide or menthofuran derivatives [33-35].
Insect repellent activity of pennyroyal was determined by using two setups (Figure 9) with citronella oil and distilled water used as positive and negative controls, respectively [32]. Setup 1 consisted of a trough with a diameter of 30 cm and a height of 12 cm. Four zones were designated within the trough (Figure 9A). The mosquitoes were introduced inside the container, and the oil sample was then injected by a syringe. Sixteen mosquitoes were observed every two minutes for a period of 20 minutes and their position within the trough was recorded. After the second minute, 75 % of the mosquitoes were found in the compartment furthest from the injection site. A gradient was achieved at this time interval and the mosquitoes moved away from the source. After the tenth minute, this compartmental difference was no longer observed, most probably due to the fact that the oil must have saturated the trough and hence there was no trend in mosquito distribution. Setup 2 consisted of a glass tube with an internal diameter of 2.5 cm and a length of 150 cm. Seven zones were designated within the tube (Figure 9B). Twenty mosquitoes were observed every two minutes for half an hour and their position recorded, following injection of the pennyroyal oil. As with setup 1, there was a statistical difference between zone 1 and zone 7 of the tube, but this difference became negligible with time. Similar results were observed with citronella. In spite of this similarity, GC-FID determination of the citronella oil revealed the presence of geraniol (60.0 %), citronellal (15.0 %) and camphene (> 15.0 %), but no significant pulegone content. With water a more random distribution of mosquitoes was observed [32].
Pennyroyal oil exhibited repellent and insecticidal effects. After 90 minutes exposure, none of the mosquitoes were airborne and those that were in contact with the oil were dead. The insect repellent activity was attributed to the high pulegone content [36].
2.4. Salvia officinalis L.
Sage contains several metabolites primarily monoterpenoids and sesquiterpenoids, diterpenoids [43], triterpenoids, such as ursolic and oleanolic acid [41, 44], and also flavonoids and phenolic glycosides [45]. The essential oil of Portuguese sage according to [46] contains α-thujone (17.4 %), α-humulene (13.3 %), 1,8-cineole (12.7 %),
Another significantly important metabolite in sage is carnosolic acid, a bitter abietane diterpenoid derivative with a carboxylic acid structure. This compound possesses antimicrobial, antioxidant, antiviral and anticancer activities [49]. Carnosolic acid was extracted using Soxhlet extraction and petroleum ether as extractant. The extract was dried and dissolved in pyridine/acetic anhydride. The neutral fraction was then chromatographed using silica gel as support [48].
Cultivation studies revealed that sage is best cultivated under shade conditions with irrigation. Propagation is best performed by cuttings every three weeks during spring after the plants have ceased to flower. The recommended planting distance is 30 cm in a row with a cultivation density of 10 plants per m2. Plants should be irrigated immediately after planting of cuttings and twice weekly in summer. The monthly harvesting of leaves produced a variable content of essential oil on fresh weight basis with the peak reached during the month of August (2.24 % v/v) and the least during December (0.52 % v/v) (Figure 11).
2.5. Verbena officinalis L.
The main constituents of
A hydromethanolic extract of the dried aerial parts of Maltese vervain was obtained by Soxhlet extraction [58]. The constitution of verbenalin was determined by HPLC using Supelcosil LC-18 column, acetonitrile/water-phosphoric acid (pH 2) gradient mobile phase with a flow rate of 1.5 ml/min. The content of verbenalin expressed as dry weight of plant material was 2.09 % (w/w). Previous reports [59] declared that contents of verbenalin were less than 0.1 % when extracted with ether but the content in methanolic extracts varied between 0.12 and 0.50 % [60].
Several pharmacological activities are attributed to vervain, namely, anti-inflammatory [54, 61], neuroprotective [62], antioxidant, antifungal [63], antileukaemic [64] and hepatoprotective [65]. Verbenalin, from Maltese vervain sources, was tested on mammalian intestinal smooth muscle
2.6. Hedera helix L.
The main constituents of
Another important group is that represented by phenolics (flavonoids, anthocyanins, coumarins and phenolic acids) [71, 73]. The essential oil from ivy stems and leaves contains germacrene D, β-caryophyllene, sabinene, β-pinene, limonene, and α-pinene [74]. Hederasaponins, from ivy grown in Malta, were extracted with 70 % ethanol by Soxhlet extraction [75]. Spring, summer, autumn and winter leaves yielded 12.75 %, 11.82 %, 10.74 % and 10.97 % (w/w) of dried extract. The hederosaponin content was determined by HPLC using Supelcosil LC- 18 column, acetonitrile/water-phosphoric acid (0.01 N) gradient mobile phase with a flow rate of 1 ml/min. Hederasaponin C and α-hederin were used as standards. The 70 % ethanolic extract contained 46.7 % hederasaponin C and 6.1 % α-hederin totaling 52.8 %. Purification of the ethanolic extract through an alumina column with methanol as solvent resulted in 62.2 % hederasaponin C and 9.2 % α-hederin. This goes in accordance with other authors [76, 77] who confirmed that hederasaponin C is the main saponin in common ivy.
The ivy leaf extracts, obtained from Maltese sources, and the standards were tested for their antimicrobial activity [75]. The tested organisms were
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0.312 | - | 1.25 – 2.50 | 0.625 – 1.25 |
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5 | - | 10 | 5 - 10 |
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2.5 | - | 5 – 10 | 5 – 10 |
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1.25 | - | 5 – 10 | 2.5 – 5 |
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2.5 | - | 5 – 10 | - |
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10 | - | - | 10 |
2.7. Crataegus monogyna Jacq.
Hawthorn contains several constituents, most of which are either pharmacologically active or have a nutritional value. Triterpenoids, flavonoids, coumarins and amines are the main groups of compounds that possess a significant activity in the treatment of cardiovascular diseases [87].
The two triterpenoids, abundantly found in hawthorns, are ursolic and oleanolic acids (figure 15). These account for 90 % of the total pentacyclic triterpenoids present in the shrub [88]. The triterpenoids oleanolic, ursolic and crataegolic acids were extracted as a crude mixture with 96 % alcohol [89, 90], as an acid-ether extract [91] and as a tincture of
Hawthorn extracts have been tested for several pharmacological activities such as antimicrobial, antioxidant [99, 100], peroxysmal tachycardia [101], prevention of cardiac necrosis [102-104], hyperglycaemia [105], atherosclerosis [106] and hypertension [107].
The hydroethanolic extract of
2.8. Calendula officinalis L.
Pharmacologically-active classes of compounds, in the marigold, include the terpenoids including the carotenoids, flavonoids, coumarins and polysaccharides [110-112]. The saponosides are particularly abundant in the plant. There are also numerous triterpenoid alcohols which are derived from tarassene, lupene, oleanene and ursene. These are present as free or esterified as monols, diols and triols. The content of monoesters of the triterpenoid diols is between 2 and 45 %, of which 1.85 % is made up of faradiol esters. The most common triterpenoid is oleanolic acid (Figure 15). The colour of the flowers is determined by the amount of carotenoids which can vary from 1.5 to 3 %. The orange flowers are made up mainly of carotenes particularly lycopene whereas the yellow flowers contain mainly xanthophylls [113]. The heterosides of quercetin and isorhamnetin (flavonoids) are present in the dry
Flowerheads of
The marigold has been investigated for its anti-microbial, anti-inflammatory [116, 117], anti-tumour [110] activities, effects on the cardiovascular and nervous systems [118, 119] as well as oestrogenic [120], hypolipidaemic [121], anti-ulcer [122] and spermicidal properties [123].
The antimicrobial activity was conducted for oleanolic acid against a number of organisms [115]. Due to the insoluble nature of oleanolic acid in water, it was incorporated in the nutrient agar for bacterial strains and in Sabouraud’s dextrose agar for fungi. In fact, [124] stated that the anti-bacterial agent was soluble in alcohol but not in water. According to the results obtained after 24 hours in the study performed, oleanolic acid was active against Gram-positive organisms (
The topical in vivo effects of oleanolic acid (2.5 %) on inflamed bites induced by mosquitoes (
2.9. Melissa officinalis L.
Lemon balm contains a volatile oil [129], flavonoids (cynaroside, rhamnocitrin, isoquercitrin, cosmosin), phenolic acids (carnosic acid and rosmarinic acid), and triterpene acids (particularly ursolic and oleanolic acid) [130]. The study by [131] focused mainly on the cultivation parameters that affect the quantity and quality of the lemon balm oil. The oil yield was 0.1 % (v/w) with cis-citral and trans-citral as the major constituents (figure 17).
Seeds were procured from four sources: Maltese (Argotti Gardens), Swiss (Basel Botanic Gardens), German A (Botanischer Garten der Martin-Luther-Universität) and German B (Botanischer Garten der RWTH). The planting distance was of 20 cm in a row with a distance of 50-60 cm between rows. The cultivation density was of 10 – 12 plants per m2. The plants were irrigated immediately after transplanting and then once every fortnight in winter but twice weekly in summer. Plots were divided into two: half treated with fertiliser (NPK Mg (12+12+17+2) + Trace elements) while the other half left untreated, as a control. The leaves were harvested in May and subjected to steam distillation extraction and GC-MS analysis. Table 4 illustrates the results obtained in this study.
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Maltese | w/o Fertiliser | 0.00 | 0.00 | 37.11 | 47.39 | 1.02 |
Fertiliser | 0.52 | 0.00 | 36.82 | 47.74 | 1.26 | |
Swiss | w/o Fertiliser | 0.55 | 0.00 | 36.08 | 48.92 | 2.11 |
Fertiliser | 1.31 | 0.55 | 30.73 | 45.13 | 2.67 | |
German A | w/o Fertiliser | 1.24 | 0.57 | 30.96 | 45.79 | 2.62 |
Fertiliser | 1.25 | 0.71 | 32.11 | 47.23 | 1.84 | |
German B | w/o Fertiliser | 1.65 | 0.56 | 31.39 | 47.63 | 2.13 |
Fertiliser | 1.31 | 0.74 | 33.42 | 49.19 | 1.94 |
In most cases, the use of fertilizer improved content of the two main terpenoids, geranial and neral. This goes in accordance with [132], stating that nitrogen fertilisers increased the yield of these constituents. In some cases citronellal also showed significant increases with fertilizer application. In another study, the oil yield was found to vary between 0.16 and 0.25% [133]. With farmyard manure, the content of neral (28.23%) and geranial (39.86%) was higher than with other treatments. Oil yield was also significantly affected by planting spacing and nutrient amendments.
2.10. Olea europea L.
The bioactive phenolic compounds present in the olive fruit include phenolic acids, phenolic alcohols, flavonoids and secoiridoids. The main phenolic acids are cinnamic, syringic, p-coumaric, vanillic, caffeic, 3,4-dihydroxyphenylacetic and protocatechuic acid [136]. Phenolic alcohols include 3,4-dihydroxyphenylethanol (hydroxytyrosol) and p-hydroxyphenylethanol (tyrosol) [137-139]. Flavonoids include taxifolin, apigenin, luteolin and lignans represented by pinoresinol and its metabolites [140]. However, an important class of metabolites found in the leaves and fruit of
Oleuropein was extracted from Maltese olives as follows [145].The leaves were defatted with petroleum ether and then extracted with 50% ethanol for 6-8 hours. The dried extract was then treated with water and sodium chloride was added until saturation was achieved. Chloroform was added and the aqueous extract was collected. Ethylacetate was added to the aqueous extract and following partitioning, the ethylacetate extract was collected. The extract was then subjected to dryness in order to obtain a yellow crystalline substance. Oleuropein in the olive leaf ethanolic extract amounts to 20.6 %, as mentioned by [146], with a content varying from 20 to 25% (w/w) total dry weight.
2.11. Urtica dubia Forsk.
Stinging nettle contains bioactive amines such as 5-hydroxytryptamine; flavonoids such as quercetin, kaempferol and their glycosides; coumarins such as scopoletin; organic acids such as caffeic acid and chlorogenic acid; fatty acids such as erucic acid, α-linolenic acid and linoleic acid; an essential oil; carotenoids such as lutein, β-carotene, neoxanthin, violaxanthin and lycopene; agglutinins such as
The stinging nettle possesses several pharmacological activities, namely antioxidant, antimicrobial, antiulcer and analgesic activities [167], anti-inflammatory effects [168] and cardiovascular effects [169]. The UDuA extracts from the Maltese
2.12. Capparis spinosa L.
According to [173], the capers contain 79% moisture, 1.6% ash, 5.8% protein, 1.6% fat and 5.4% raw fibre. It contains several minerals such as, Ca (871 ppm), Mg (636 ppm), K (542 mg/100mL), Na (226 ppm), Fe (13 ppm) and P (21 mg/100g). Other valuable constituents include the flavonoids such as rutin, kaempferol and its glycosides; alkaloids (Figure 20) such as cadabicine [174], capparisine A, capparisine B, capparisine C; 2-(5-hydroxymethyl-2-formylpyrrol-1-yl) propionic acid lactone and N-(3′-maleimidy1)-5-hydroxymethyl-2-pyrrole formaldehyde [175]. Other constituents include aldehydes, esters, sesquiterpenes, monoterpenes and sulphur compounds with methyl-isothiocyanate as the main constituent [176], carotenoids with lutein as the main constituent [177], sterols such as β-sitosterol, campesterol, stigmasterol, 5-avenasterol, cholesterol and campestanol [178], and a lectin (
Metabolites from the Maltese caper plant were obtained by extracting the plant material with four different solvents [180]. The xanthoproteic test for proteins [181], Fehling’s test for carbohydrates, Sudan IV test for fats and lipids [182], Dragendorff’s test for alkaloids [183], triphenyltetrazolium test for terpenoids and the acidified vanillin test for flavonoids [184] were carried out on the extracts. The petroleum ether extract (0.020 % w/w plant material) contained fats and lipids, the aqueous/methanol extract (2.401 % w/w plant material) contained proteins and terpenoids, the methanol extract (1.398 % w/w plant material) contained alkaloids, while the aqueous extract (3.015 % w/w plant material) contained carbohydrates and terpenoids.
The caper plant was tested for several pharmacological activities such as antiviral [185], anti-arthritic [186], anti-oxidant [187], hypolipidaemic [188], antihyperglycaemic [189], chondrocyte protective [190], antiallergic, antihistaminic [191], antifungal [192], anti-Leishmania [193] and antimicrobial [194]. The Brine shrimp test was conducted for the extracts derived from the Maltese caper plants [180]. Briefly, Artemia salina eggs were hatched and challenged with various concentrations of the extracts ranging between 0.0001 and 1 % as 1 in 10 dilutions. After 24 hour the number of dead larvae (nauplii) was determined. The aqueous extract exhibited the lowest LC50 (0.014%) compared to the methanol (0.0475%) and the aqueous/methanol (0.08%) extracts. The chloroform extract did not reach a 50% lethal effect and therefore the LC50 could not be determined. According to [195] the methanol, aqueous and aqueous/methanol extracts were all active as their LC50 was below the 0.1% threshold.
2.13. Ephedra fragilis Desf.
Aerial parts of local cultivated
Clinically, ephedra has been tested for its anti-hypertensive [203], bronchodilator [204], decongestant [205], diuretic [206] and immune booster [207]. The immunomodulatory response of ephedrine and the
2.14. Nicotiana glauca RC Graham
Tree tobacco contains pyridine alkaloids [212], as for other
The leaves of Maltese
The nicotine and anabasine have been widely used as pesticides. Nicotine is a powerful insecticide towards aphids [221] and larvae of lepidopterous pests [222]. Anabasine and nicotine exert their insecticidal effect by interacting with nicotinic acetylcholine receptors [222, 223]. Anabasine and
3. Conclusion and further directions
The studies on the fourteen Maltese medicinal plants, presented herein, demonstrate a wide array of experimental work that is all associated with phytochemical research. This is a very small fraction of the Maltese medicinal flora, but in terms of research, this represents a diversity of research protocols that may be adopted for medicinal plant research. In some cases, phytochemical analysis is the end-point of the research whereas in others, phytochemical analysis leads on to further studies, including pharmacological testing.
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