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Exploitation of Chinese Upland Cotton Cultivar Germplasm Resources to Mine Favorable QTL Alleles Using Association Mapping

Written By

Hongxian Mei, Xiefei Zhu, Wangzhen Guo, Caiping Cai and Tianzhen Zhang

Submitted: 18 February 2014 Published: 02 July 2014

DOI: 10.5772/58587

World Cotton Germplasm Resources IntechOpen
World Cotton Germplasm Resources Edited by Ibrokhim Y. Abdurakhmonov

From the Edited Volume

World Cotton Germplasm Resources

Edited by Ibrokhim Y. Abdurakhmonov

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1. Introduction

Cotton (Gossypium spp.) is the most important natural textile fiber source globally. The worldwide economic impact of the cotton industry is estimated at approximately $500 billion per year with an annual utilization of about 27 million metric tons of cotton fiber [1].The tetraploid species Gossypium hirsutum L. (n=26, AD genome), commonly referred to as Upland cotton, accounts for 95% of the world’s cotton production [2]. Most of the objectives in cotton breeding, such as yield, fiber quality, biotic-and abiotic-stress tolerance, are all complex traits, which are controlled by a large number of quantitative trait loci (QTLs). It is becoming progressively more difficult to improve these traits using conventional breeding methods due to their complex architecture and inheritance [3]. Fortunately, the development in applied genomics research has provided alternative tools to improve efficiency in plant breeding programs. Molecular markers tightly linked to the target genes or QTLs can be used for marker-assisted selection (MAS) and/or genomic selection (GS) [4-5]. In the past two decades, the availability of abundant molecular markers has made tagging QTL harboring functional genes through family-based linkage mapping a routine process, and a large number of QTLs for fiber quality properties [6-40], yield and its components [6, 8, 10-11, 14, 18-19, 27, 30-32, 36, 40-45], nematode resistance [46-49], Verticillium wilt resistance [50-53] as well as Fusarium wilt resistance [54-60] have been identified in cotton.

However, approximately 80% of the previously reported QTLs could not be confirmed in subsequent studies, and few have actually been applied in breeding programs [61-62]. This may be because most QTLs were population-specific, and the genetic variations detected in a unique bi-parental population were not shared with other genetic populations, or shared but fixed in the parental lines. In addition, limited genetic recombination events in most populations used for linkage mapping make it difficult to map QTL with a high resolution, which severely limits their application in breeding programs. With the potential to exploit all recombination events that occurred in the evolutionary history of natural populations, linkage disequilibrium (LD) based association mapping (AM) has become a powerful approach for the dissection of complex traits and identification of causal variation with modest effects for target traits in many plant species [3, 63] including cotton [64-73]. Furthermore, although AM has been successfully used to detect the QTLs underlying quantitative traits in some crops, from a breeding standpoint, detecting associated loci is just the first step; analyzing the genetic effects of alleles and identifying favorable alleles will be more beneficial for target trait improvement. For example, Breseghello and Sorrells identified several potentially beneficial alleles for kernel size and milling quality by comparing the average phenotypic value with specific alleles and null alleles in a soft winter wheat population [74]; Jia et al. identified some putative resistant alleles for Sheath Blight resistance in a rice panel composed of 217 accessions from the USDA core collection, and found that the number of putative resistant alleles presented in an entry was highly and significantly correlated with the decrease of ShB rating [75].

China is the world’s largest cotton-growing nation, but not a cotton domestication region. Most Upland cotton cultivars developed in China were derived from a few germplasm resources such as Deltapine (DPL), Stoneville (STV), Foster, King and Uganda, all of which were introduced from abroad [76]. More than 8800 accessions have been collected, developed, and maintained in Chinese cotton germplasm collection [77]. Among these collections, G. hirsutum varieties and breeding lines comprise about 83% of the total. Current and obsolete cultivars have been and continue to be the main resources for cotton breeding programs in China. Dissecting the genetic basis of main breeding objective traits will be of great benefits to germplasm evaluation and future molecular breeding. In this chapter, we reviewed QTLs underlying yield and its components, fiber quality properties and Fusarium wilt resistance detected by association mapping and their favorable alleles identified in 2 Chinese Upland cotton AM panels [71-73]. We hope that these results should provide useful information for further understanding the genetic basis of these traits, and would facilitate future breeding process by MAS in Upland cotton.

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2. Favorable QTL alleles for yield and its components

In recent years, demand for cotton fiber in the world market has dramatically increased. While cotton acreage has declined worldwide in the past few years, mainly it was due to strong competition from other crops as well as production costs [1]. To improve lint yield of Upland cotton cultivars will remain critical for meeting worldwide demand and maintaining profitability for cotton growers. A total of 356 representative Upland cotton cultivars and breeding lines were selected from the cotton germplasm collection in Cotton Research Institute, Chinese Academy of Agricultural Sciences (CRI-CAAS), and from the collection in our laboratory, and assembled to construct an AM panel. The population consisted of 348 cultivars developed in China, seven introduced from the U.S. including the genetic standard line TM-1, and one introduced from Uganda. According to their release year, the 348 Chinese cultivars could be divided into the following six groups: I (1930–1960, 26 lines); II (1961–1970, 26 lines); III (1971–1980, 39 lines), IV (1981–1990, 83 lines); V (1991–2000, 125 lines); and VI (2000–2005, 49 lines). The cultivars introduced from abroad, DPL 15, DPL 16, STV 2B, King, Foster 6 and Uganda 3, were used as a check group for genetic diversity and allele transmission evaluation, because they had been used as the main founder parents in China’s Upland cotton breeding programs [71]. Yield and its components of the accessions were evaluated in three diverse environments, including lint yield per plant (LP), seed cotton yield per plant (SY), bolls per plant (BN), boll weight (BW), lint percentage (LP), lint index (LI) and seed index (SI). Three hundred and eight-one pairs of SSR primers that amplify loci evenly covering the tetraploid cotton genome [78] were selected to genotype the 356 accessions.

The Bayesian model-based program STRUCTURE 2.3 was used to infer the population structure using 66 unlinked or weakly linked SSR markers as described in reference [79]. The length of the burn-in period and the number of Markov Chain Monte Carlo replications after burn-in were all assigned at 100,000 with an admixture and allele frequencies correlated model. Five independent run iterations were performed with the hypothetical number of subpopulations (k) ranging from 1 to 10. However, the result showed that the LnP(D) value corresponding to each hypothetical k kept increasing with k value and did not show any peak (Figure 1). Thus an ad hoc statistic Δk [80] was joined to correctly estimate the population structure. The Δk value showed a much higher likelihood at k=2 than at k=3-10 (Figure 1), suggesting that the total panel could be divided into 2 major subpopulations. Based on the correct k, all accessions were assigned to each of the two subpopulations (P1 and P2), for which the membership value (Q value) was >0.5, and the population structure matrix (Q) was generated for further association mapping. The P1 group contained 115 accessions including 63 cultivars from Yellow River cotton growing region, 46 lines from North and Northwest China regions, and 6 cultivars from Yangtze River region. The P2 group consisted of 241 accessions including 116 lines from Yellow River region, 107 lines from Yangtze River region, 10 lines from the North and Northwest China regions, and 8 lines introduced from abroad (see reference [71] for details). The software SPAGeDi was used to calculate the pair-wise relatedness [81]. For the kinship coefficient values, 86.85% was less than 0.05, 8.56% had a range of 0.05–0.10, and the remaining 4.59% showed various degrees of genetic relatedness. Based on the results of the relatedness analysis, a K matrix was constructed for further association mapping.

Figure 1.

Estimated LnP(D) and ∆k over five repeats of STRUCTURE analysis (a) LnP(D) for k from 1 to 10 for 356 accessions. Note that LnP(D) value of each hypothetical k kept increasing with k value. (b) ∆k for k from 2 to 9 for 356 accessions. The Δk value showed a much higher likelihood at k=2 than at k=3–10, suggesting that the total panel should be divided into two major subpopulations

Marker-trait association analysis were performed with the MLM model, considering both kinship (K) and population structure (Q), implemented in TASSEL software [82]. At the α=0.01 (-logP=2) level, a total of 195 significant associations were detected between 82 SSR markers and 7 lint yield traits. Among these, most of the associations (125 of 195) were detected in only one environment, and the proportion of phenotypic variation explained by markers ranged from 1.52% to 9.40%, with an average of 3.70% (see reference [71] for details). After Bonferroni correction [83], 55 associations were found to be significant (P≤0.05/145,-logP≥3.46) between 26 SSR markers and 7 lint yield traits (Table 1). Most of the associations could be detected in more than one environment, and the proportion of phenotypic variation explained by markers ranged from 1.63% to 9.40%, with an average of 4.51%. The number of SSR markers associated with LY, SY, BN, BW, LP, LI and SI were 9, 4, 6, 4, 14, 17 and 1, respectively. Seventeen loci were co-associated with two or more different traits (Table 1), for example, NAU3269 (Chr. 5) and NAU3100 (Chr. 23) were simultaneously associated with FY, SY, BN, LP, and LI, and most of the lint yield-associated loci were associated with at least one of its components. These associations coincided with phenotypic correlations among these traits [71]. This might result from pleiotropy of a single causal gene or tight linkage of multiple causal genes. We found that 10 of 14 markers associated with LP were detected in all three environments, which was consistent with the results in phenotypic evaluation that LP possessed the highest broad-sense heritability (hB2=75.77%) [71]. The phenotype of complex traits often result from the combined actions of multiple genes and environmental factors [3]; only those traits with high heritability can be stably detected. The resulting stably associated markers should be useful for cotton breeding with broad adaptability to different environments.

Traits Marker loci Chr. Position -LogP R2(%)
E1 E2 E3 E1 E2 E3
LY NAU3269 A05(Chr.05) 182.215 3.43 4.42 3.33 2.83 4.60 3.05
NAU5166 A10(Chr.10) 23.423 ns 5.49 ns 5.40
NAU2935 A10(Chr.10) 51.592 ns 4.00 ns 4.72
JESPR204 A13(Chr.13) 59.714 4.12 3.00 ns 4.60 4.08
CIR246 D02(Chr.14) 112.473 ns ns 3.55 4.44
BNL3594 D06(Chr.25) 7.66 ns 4.51 ns 7.65
TMK19 D06(Chr.25) 70.38 4.30 2.77 3.97 4.21 3.21 4.28
NAU3100 D09(Chr.23) 25.262 3.67 5.38 4.31 4.08 7.08 5.41
NAU2776 D10(Chr.20) 9.452 ns 3.81 2.54 4.41 2.76
SY NAU3269 A05(Chr.05) 182.215 2.25 4.16 2.43 1.85 4.41 2.19
CIR246 D02(Chr.14) 112.473 ns ns 4.23 5.36
BNL3594 D06(Chr.25) 7.66 ns 4.61 ns 7.97
NAU3100 D09(Chr.23) 25.262 ns 4.03 2.82 5.61 3.84
BN NAU6584 A03(Chr.03) 74.975 3.53 2.29 ns 3.93 2.91
NAU3269 A05(Chr.05) 182.215 2.08 3.52 3.45 1.74 3.64 3.44
BNL3594 D06(Chr.25) 7.66 ns 3.62 ns 6.67
TMK19 D06(Chr.25) 70.38 3.58 ns 2.41 3.71 2.84
NAU493 D07(Chr.16) 113.413 3.91 ns ns 3.38
NAU3100 D09(Chr.23) 25.262 ns 3.48 ns 4.92
BW BNL1414 A09(Chr.09) 95.911 ns 3.26 3.72 4.18 4.45
NAU4047 A12(Chr.12) 11.118 3.29 4.17 ns 3.32 4.61
NAU3398 A13(Chr.13) 3.311 3.47 3.46 ns 5.65 6.03
JESPR208 D09(Chr.23) 118.225 ns 4.01 3.69 5.15 4.45
LP NAU3269 A05(Chr.05) 182.215 3.54 2.21 2.72 3.18 2.02 2.59
NAU5166 A10(Chr.10) 23.423 2.33 4.36 2.74 1.80 4.10 2.44
NAU2508 A10(Chr.10) 128.028 4.45 3.62 4.68 5.23 4.81 6.09
NAU980 A11(Chr.11) 0 3.40 ns 3.75 5.65 6.72
JESPR135 A11(Chr.11) 55.787 3.79 6.64 3.80 3.16 6.45 3.54
NAU3398 A13(Chr.13) 3.311 3.50 ns ns 4.99
JESPR204 A13(Chr.13) 59.714 3.73 2.96 2.22 4.41 3.96 3.11
BNL3590 D03(Chr.17) 39.284 4.70 ns 2.89 4.91 338
TMK19 D06(Chr.25) 70.38 5.97 4.58 3.67 6.04 5.18 4.19
NAU3100 D09(Chr.23) 25.262 4.50 3.35 2.89 5.35 4.58 4.00
NAU3917 D10(Chr.20) 31.125 ns 3.57 ns 4.03
BNL1404 D11(Chr.21) 33.571 4.48 6.20 3.51 3.81 6.01 3.23
Gh508 D11(Chr.21) 54.48 2.15 3.37 3.81 1.63 3.06 3.54
NAU2361 D11(Chr.21) 101.215 4.05 4.30 4.03 6.37 7.34 7.03
LI NAU3269 A05(Chr.05) 182.215 4.98 2.77 2.38 4.92 2.63 2.32
NAU980 A11(Chr.11) 0 8.85 2.34 2.80 8.96 4.76 5.72
JESPR135 A11(Chr.11) 55.787 ns 5.43 2.34 5.20 2.09
Gh369 A11(Chr.11) 84.701 3.77 2.09 ns 4.79 3.00
NAU1151 A12(Chr.12) 97.965 6.08 ns ns 5.63
NAU3398 A13(Chr.13) 3.311 6.01 2.49 ns 8.19 4.15
JESPR204 A13(Chr.13) 59.714 ns 3.68 ns 4.81
CIR246 D02(Chr.14) 112.473 4.75 3.14 ns 5.77 4.17
BNL3590 D03(Chr.17) 39.284 3.30 2.70 3.47 3.67 3.13 4.26
NAU2233 D05(Chr.19) 171.278 4.86 2.15 ns 5.96 3.04
TMK19 D06(Chr.25) 70.38 4.83 4.02 ns 5.19 4.55
NAU3100 D09(Chr.23) 25.262 5.90 2.74 3.47 7.19 3.84 4.93
NAU2776 D10(Chr.20) 9.452 ns ns 3.57 4.30
NAU3917 D10(Chr.20) 31.125 ns 3.66 2.03 4.13 2.45
BNL1404 D11(Chr.21) 33.571 ns 5.85 2.72 5.64 2.50
Gh508 D11(Chr.21) 54.48 ns 3.67 3.41 3.38 3.27
NAU2361 D11(Chr.21) 101.215 6.27 5.16 4.58 9.40 8.45 8.12
SI NAU493 D07(Chr.16) 113.413 3.70 2.85 5.24 3.85 2.90 5.75

Table 1.

SSR marker loci significantly associated with lint yield traits and their explained proportion of phenotypic variation in 3 different environments. LY: lint yield (g/plant); SY: seed cotton yield (g/plant); BN: bolls per plant; BW: boll weight (g); LP: lint percentage (%); LI: lint index (g/100 seeds); SI: seed index (g/100 seeds); E1: Jiangpu in 2009; E2: Dafeng in 2010; E3: Zhengzhou in 2010

To identify favorable alleles, the phenotypic allele effect was estimated through comparison between the average phenotypic value over accessions with the specific allele and that of all accessions:

ai=∑xij/ni−∑Nk/nk

where ai is the phenotypic effect of the ith allele; xij is the phenotypic value over the jth accession with the ith allele; ni is the number of accessions with the ith allele; Nk is the phenotypic value over all accessions; nk is the number of accessions. If the value of ai > 0, the allele is considered to have a positive effect, if it is < 0, it corresponds to a negative allele. The favorable alleles were then identified according to the breeding objective of each target trait [69, 71]. Phenotypic effects of each QTL allele for the 41 associated loci detected in more than one environment were measured, and 5, 2, 3, 4, 12, 14 and 1 favorable alleles for FY, SY, BN, BW, LP, LI and SI were identified, respectively. Phenotypic effects and representative accessions for each favorable allele are shown in Table 2. Among the favorable alleles, NAU3100-2 had the most positive phenotypic effect for FY and SY, and increased FY and SY by 3.61 g and 7.27 g, respectively; NAU6584-2, NAU3398-2, NAU5166-2 and NAU3917-2 increased BN, BW, LP and LI by 0.89, 0.42 g, 4.93% and 0.94 g, respectively; while NAU493-1 deceased SI by 0.17 g. Lint yield of cotton is the result of series components and their interactions, such as boll number, boll weight, lint percentage, lint index, and seed index. Developing potentially high-yielding cultivars thus relies to some extent on selecting the appropriate yield components. As some of the QTLs were associated with more than one yield component, favorable alleles must be treated with caution. Positively co-associated genetic loci could simultaneously improve multiple target traits, while negative linkages must be broken.

Traits Favorable allele ai Accessions Representative accessions
LY NAU3269-2 0.27 133 Simian3, Zhongmiansuo9, Huakangmian1
JESPR204-1 0.70 314 Simian3, Zhongmiansuo9, P164-2
TMK19-2 1.02 240 Simian3, Zhongmiansuo9, P164-2
NAU3100-2 3.61 87 Simian3, Zhongmiansuo9, Lumianyan16
NAU2776-1 0.85 151 Zhongmiansuo9, P164-2, Lumianyan16
SY NAU3269-2 0.42 133 Zhongmiansuo9, Zhongmiansuo19, Simian3
NAU3100-2 7.27 87 Zhongmiansuo9, Han4849, Lumianyan16
BN NAU6584-2 0.89 217 Lumianyan16, Zhongmiansuo44, Zhongmiansuo9
NAU3269-2 0.08 133 Zhongmiansuo9, Wanmian73-10, Simian3
TMK19-2 0.45 235 Zhongmiansuo44, Zhongmiansuo9, Wanmian17
BW BNL1414-2 0.18 93 Zhongmiansuo18, Zhongmiansuo5, I40005
NAU4047-2 0.03 221 Zhongmiansuo18, Zhongmiansuo5, I40005
NAU3398-2 0.42 22 Zhongmiansuo5, I40005, Hua101
JESPR208-2 0.20 86 Zhongmiansuo18, Zhongmiansuo5, I40005
LP NAU3269-2 0.23 133 Simian3, Ekangmian9, Huakangmian1
NAU5166-2 4.93 8 Simian3, Huakangmian1, Sumian4
NAU2508-2 0.36 113 Nannongzao, 86-1, Yu668
NAU980-3 2.79 9 Ekangmian6, Emian16, Ekangmian10
JESPR135-1 0.13 343 XiangSC-24, Simian3, Ekangmian9
JESPR204-1 0.36 309 XiangSC-24, Simian3, Ekangmian9
BNL3590-1 0.26 327 XiangSC-24, Simian3, Ekangmian9
TMK19-2 0.58 235 XiangSC-24, Simian3, Huakangmian1
NAU3100-2 1.55 86 Simian3, Ekangmian9, Nannongzao
BNL1404-1 0.13 343 XiangSC-24, Simian3, Ekangmian9
Gh508-1 0.07 347 XiangSC-24, Simian3, Ekangmian9
NAU2361-3 0.75 73 Ekangmian9, Yu668, Yumian21
LI NAU3269-2 0.01 133 Huakangmian1, I40005, Ekangmian9
NAU980-3 0.84 9 I40005, Zhongmiansuo5, Hua101
JESPR135-1 0.02 343 Huakangmian1, Emian23, I40005
Gh369-3 0.11 8 Emian16, Ekangmian8, Yumian20
NAU3398-2 0.80 22 Huakangmian1, I40005, Zhongmiansuo5
CIR246-3 0.63 16 Hua101, Zhongmiansuo41, Yumian9
BNL3590-1 0.06 327 Huakangmian1, Emian23, I40005
NAU2233-1 0.01 212 Huakangmian1, Emian23, I40005
TMK19-2 0.10 235 Huakangmian1, Emian23, I40005
NAU3100-2 0.38 86 I40005, Zhongmiansuo5, Hua101
NAU3917-2 0.94 6 Huakangmian1, Simian4, Sumian9
BNL1404-1 0.03 343 Huakangmian1, Emian23, I40005
Gh508-1 0.01 347 Huakangmian1, Emian23, I40005
NAU2361-3 0.28 73 Emian23, I40005, Zhongmiansuo5
SI NAU493-1 -0.17 230 Chaoyangmian1, Xuzhou1818, XiangSC-24

Table 2.

Favorable QTL alleles, their phenotypic effects (ai) and representative accessions. LY: lint yield (g/plant); SY: seed cotton yield (g/plant); BN: bolls per plant; BW: boll weight (g); LP: lint percentage (%); LI: lint index (g/100 seeds); SI: seed index (g/100 seeds); Representative accessions are the top-3 entries for the target trait value of accessions with the corresponding favorable allele

Allele frequencies of the 23 favorable alleles in the CK group and the six Chinese historically released cultivar groups are summarized in Table 3. Based on allele frequencies across the different groups, these favorable alleles could be categorized into three classes. The alleles in the first class, such as JESPR135-1, BNL1404-1 and Gh508-1, presented in the founder cultivars and with high frequency in all populations, might have been passed down stably from the original parents and were almost fixed in modern cultivars by selection. Alleles in the second class, such as BNL3269-2, BNL1414-2, NAU3100-2 and JESPR208-2, presented in the founder cultivars and with moderate to low frequency in most populations, should have been underutilized in modern breeding programs. Those in the third class, such as NAU5166-2, NAU980-3, Gh369-3 and CIR246-3, not presented in the founder cultivars and presented at low frequency in modern cultivars, might be from other original parents or could have been generated by mutations and/or recombinations. Favorable alleles, especially of the latter two classes, should have a great potential in future Upland cotton genetic improvement. We suggest that a multi-parent population should be constructed using cultivars that possess most of the favorable alleles, and in the meantime, a ranking system for MAS or genomic selection should be developed based on the results of AM. Favorable alleles that were passed down from the founder parents and have been almost fixed in modern cultivars formed the basis of lint yield of Chinese Upland cotton, and should be treated as fundamental elements in order to reject deleterious alleles at the corresponding loci. Alleles either absent in the founder cultivars or present at moderate to low frequencies in most cultivar groups have been underutilized in modern breeding programs, and should be regarded as essential elements for increasing lint yield potential.

Favorable alleles CK I II III IV V VI Total
NAU6584-2 0.3333 0.5000 0.6923 0.7692 0.6747 0.4720 0.7755 0.6096
NAU3269-2 0.6667 0.5769 0.5385 0.6154 0.3976 0.3040 0.1020 0.3736
BNL1414-2 0.5000 0.3077 0.1923 0.4872 0.2410 0.2320 0.1633 0.2612
NAU5166-2 0.0000 0.0000 0.0000 0.0500 0.0000 0.0320 0.0417 0.0225
NAU2508-2 0.4000 0.1200 0.2400 0.2250 0.3012 0.3952 0.4694 0.3333
NAU980-3 0.0000 0.0200 0.0000 0.0256 0.0244 0.0369 0.0000 0.0233
JESPR135-1 1.0000 0.8462 1.0000 1.0000 0.9759 0.9840 1.0000 0.9775
Gh369-3 0.0000 0.0000 0.0000 0.0000 0.0241 0.0480 0.0000 0.0225
NAU4047-2 0.6667 0.5000 0.5769 0.6154 0.5663 0.6400 0.7551 0.6208
NAU3398-2 0.0000 0.0385 0.0000 0.1026 0.0843 0.0720 0.0204 0.0618
JESPR204-1 1.0000 0.8462 0.8077 0.7692 0.8675 0.9200 0.9388 0.8820
CIR246-3 0.0000 0.0000 0.0000 0.0256 0.0241 0.0800 0.0612 0.0449
BNL3590-1 0.5000 0.8077 0.8462 0.8462 0.9036 0.9440 0.8776 0.8904
NAU2233-1 0.6667 0.4231 0.5000 0.3590 0.5542 0.6560 0.8163 0.5955
TMK19-2 0.6667 0.5000 0.5769 0.6667 0.6024 0.7280 0.8163 0.6742
NAU493-1 0.6667 0.5000 0.7308 0.6923 0.6265 0.6800 0.5714 0.6461
NAU3100-2 0.3333 0.2308 0.0769 0.2308 0.2289 0.2400 0.3673 0.2444
JESPR208-2 0.5000 0.3077 0.1154 0.4359 0.2289 0.2240 0.1633 0.2444
NAU2776-1 0.1667 0.4231 0.3462 0.5385 0.4458 0.4480 0.3061 0.4242
NAU3917-2 0.0000 0.0000 0.0000 0.0513 0.0000 0.0240 0.0204 0.0169
BNL1404-1 1.0000 0.8462 1.0000 1.0000 0.9759 0.9840 1.0000 0.9775
Gh508-1 1.0000 0.9231 1.0000 0.9750 1.0000 0.9920 1.0000 0.9888
NAU2361-3 0.2143 0.0769 0.0417 0.1538 0.1125 0.2810 0.3673 0.2066

Table 3.

Allele frequency for each favorable QTL allele in historically released Chinese Upland cotton cultivar groups. CK, I, II, III, IV, V and VI indicates the founder parent group (CK), and the Chinese cultivars released in 1930–1960, 1961–1970, 1971–1980, 1981–1990, 1991–2000 and 2000–2005, respectively

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3. Favorable QTL alleles for fiber quality traits

With the acceleration of spinning speed, the demand for cotton fiber quality is increasing rapidly [9, 17]. It is important to elucidate the molecular genetics of Upland cotton fiber qualities, and such information would enable the subsequent improvement of cotton cultivars by pyramiding favorable alleles of fiber quality traits. We performed a marker-trait association mapping for fiber quality traits using MLM model implemented in TASSEL [82] with the panel mentioned above [71]. At the α=0.01 (-logP=2) level, a total of 59 significant associations were detected between 41 SSR markers and 5 fiber quality traits (Table 4). Among these, almost all of the associations were detected in only one environment, and the proportion of phenotypic variation explained by markers ranged from 1.84% to 7.12%, with an average of 3.53% (Mei et al. unpublished data). If a more stringent threshold with Bonferroni correction [83] was adopted, only 9 associations were found to be significant (Table 4). The result might be caused by low diversity of fiber quality properties existed in the 356-accession panel. Although the yield potential of Chinese cultivars are equal to or a bit higher than those developed in the United States or Australia, the fiber quality traits of Chinese cultivars are not as good as those of American or Australian cultivars [73, 84]. Narrow variation in fiber qualities of Chinese cultivars had severely limited the marker-trait association detection power in association mapping.

Traits Marker loci Chr. Position -LogP R2 (%)
E1 E2 E3 E1 E2 E3
FL NAU845 A07(Chr.07) 54.589 4.16 ns ns 5.91
NAU3398 A13(Chr.13) 3.311 ns 3.15 ns 5.64
BNL1707 A13(Chr.13) 104.694 2.39 ns ns 2.27
JESPR152 D01(Chr.15) 110.252 2.59 ns ns 3.25
BNL3359 D06(Chr.25) 140.397 4.3 ns ns 5.67
JESPR297 D07(Chr.16) 43.109 ns 2.18 ns 2.08
NAU5418 D11(Chr.21) 75.156 ns 2.08 ns 4.47
BNL3537 D12(Chr.26) 39.496 ns ns 2.06 2.77
NAU2980 D13(Chr.18) 0 ns ns 2.02 2.64
FS NAU6584 A03(Chr.03) 74.975 2.3 ns ns 3.16
NAU2561 A05(Chr.05) 56.73 2.96 ns ns 3.08
NAU3269 A05(Chr.05) 182.215 3.53 ns ns 3.88
NAU3427 A06(Chr.06) 5.598 2.25 ns ns 2.91
NAU3654 A07(Chr.07) 74.363 ns 3.02 ns 4.36
NAU5166 A10(Chr.10) 23.423 2.09 ns ns 1.95
BNL1707 A13(Chr.13) 104.694 5.59 ns ns 6.04
BNL2646 D01(Chr.15) 75.614 ns 2.15 ns 2.56 3.44
NAU2901 D01(Chr.15) 104.568 2.21 ns ns 2.09
NAU1070 D02(Chr.14) 13.885 3.3 ns ns 4.3
BNL1606 D03(Chr.17) 50.883 ns 2.27 ns 2.92
NAU2233 D05(Chr.19) 171.278 2.15 ns ns 3.42
BNL3594 D06(Chr.25) 7.66 ns 2.41 ns 5.4
BNL3359 D06(Chr.25) 140.397 3.16 ns ns 4.23
NAU6468 D07(Chr.16) 83.101 2.44 ns ns 2.36
FF JESPR304 A02(Chr.02) 22.517 ns ns 2.16 3.41
NAU862 A03(Chr.03) 9.309 2.24 ns ns 2.21
BNL3452 A05(Chr.05) 188.717 2 ns ns 3.14
NAU3427 A06(Chr.06) 5.598 3.82 ns ns 4.72
NAU2935 A10(Chr.10) 51.592 2.55 ns ns 3.2
JESPR135 A11(Chr.11) 55.787 2.34 ns ns 2.15
JESPR204 A13(Chr.13) 59.714 3.81 ns ns 5.36
BNL2646 D01(Chr.15) 75.614 2.54 ns ns 3.8
BNL1604 D07(Chr.16) 36.152 ns 2.85 ns 5.43
JESPR297 D07(Chr.16) 43.109 ns 2.03 ns 1.89
BNL1694 D07(Chr.16) 52.03 2.11 ns ns 3.2
BNL1404 D11(Chr.21) 33.571 2.72 ns ns 2.58
Gh508 D11(Chr.21) 54.48 3.71 ns ns 3.68
FU NAU3254 A01(Chr.01) 33.644 2.02 ns ns 2.61
NAU2161 A03(Chr.03) 0 ns ns 2.44 3.83
NAU3654 A07(Chr.07) 74.363 ns 2.26 ns 3.29 0
Gh486 A09(Chr.09) 10.554 ns ns 2.24 4.23
NAU5428 A11(Chr.11) 155.464 3.91 ns ns 7.12
BNL1231 A11(Chr.11) 169.199 2.77 ns ns 2.73
BNL3261 A12(Chr.12) 40.276 ns 2.08 ns 4.45
BNL1606 D03(Chr.17) 50.883 ns 2.48 ns 3.2
JESPR297 D07(Chr.16) 43.109 ns ns 2.57 2.53
Gh501 D13(Chr.18) 55.386 2.17 ns ns 2.8
FSCI NAU2561 A05(Chr.05) 56.73 2.33 ns ns 2.31
NAU5428 A11(Chr.11) 155.464 2.72 ns ns 5.34
BNL1707 A13(Chr.13) 104.694 3.58 ns ns 3.68
NAU2901 D01(Chr.15) 104.568 2.46 2.27 ns 2.36 2.17
JESPR152 D01(Chr.15) 110.252 3.45 ns ns 4.34
NAU1070 D02(Chr.14) 13.885 3.19 ns ns 4.15 1.99
BNL1606 D03(Chr.17) 50.883 ns 2.52 ns 3.23
BNL3594 D06(Chr.25) 7.66 ns 2.29 ns 3.64 5.2
BNL3359 D06(Chr.25) 140.397 3.19 ns ns 4.25
NAU6468 D07(Chr.16) 83.101 2 ns ns 1.84
JESPR208 D09(Chr.23) 118.225 ns 2 ns 2.61
NAU2776 D10(Chr.20) 9.452 ns 2.11 ns 2.74

Table 4.

SSR loci significantly (-logP≥2.0) associated with fiber quality traits detected in the 356 Chinese Upland cotton cultivars. FL: fiber length; FS: fiber strength; FF: fiber fineness measured by micronaire value; FU: fiber uniformity ratio; FSCI: fiber spinning consistency index; E1: Jiangpu in 2009; E2: Dafeng in 2010; E3: Zhengzhou in 2010

To embrace the genetic diversity of fiber qualities as large as possible, a previous collection comprising 99 Upland cotton cultivars and breeding lines was used for further association mapping. The collection included 63 cultivars and 36 breeding lines with elite fiber quality traits (13 of which were PD lines introduced from the United States), and the fiber properties including fiber length, strength, and fineness were evaluated in 2004 and 2007. A total of 260 SSR markers, including the markers linked to fiber properties reported in previous studies [17-19], were used to screen the 99 accessions, and 97 of which showed polymorphisms [73]. Marker-trait association analysis was performed with the MLM model implemented in TASSEL [82]. At the α=0.01 (-logP=2) level, a total of 51 significant associations were detected between 33 SSR markers and 3 lint yield traits, and 7 of the these associations could be detected in both 2 years. The proportion of phenotypic variation explained by markers ranged from 7.76% to 23.99%, with an average of 14.07%. After Bonferroni correction [83], 17 associations were still significant (P≤0.05/97,-logP≥3.29). Compared with the results from our previous 356-accession panel, both the number of significant associations and the proportion of phenotypic variation explained by markers were improved in the current panel.

Traits Marker loci Chr. Position -logP R2
2004 2007 2004 2007
Fiber length NAU934 A05(Chr.05) 186.594 2.32 ns 7.83
NAU1200 A05(Chr.05) 3.42 ns 17.4
NAU1037 A08(Chr.08) 53.526 ns 3.50 17.83
BNL1317 A09(Chr.09) 88.456 2.62 2.68 12.18 14.12
NAU2354 A09(Chr.09) 132.251 ns 2.06 10.4 13.32
JESPR295 A12(Chr.12) 32.732 2.52 2.92 11.56 15.17
NAU445 A12(Chr.12) 123.764 2.21 ns 10.22
JESPR153 A13(Chr.13) 63.046 3.00 ns 13.52
NAU2272 D02(Chr.14) 55.105 ns 2.64 16.26
NAU1102 D05(Chr.19) 2.01 ns 9.32
NAU1302 D08(Chr.24) 72.101 ns 3.03 18.24
BNL1521 D08(Chr.24) 73.477 ns 3.88 16.21
JESPR78 D08(Chr.24) 75.81 ns 3.88 16.21
NAU1336 D08(Chr.24) 78.13 ns 3.88 16.21
NAU1262 D08(Chr.24) 88.599 ns 3.88 16.21
JESPR127 D08(Chr.24) 97.939 ns 3.88 16.21
NAU1004 D09(Chr.23) 13.67 2.64 ns 12.23
TMO06 D09(Chr.23) 109.242 3.78 3.88 16.86 19.56
NAU904 D10(Chr.20) 53.078 ns 2.14 13.88
NAU2443 D13(Chr.18) 62.214 2.30 ns 7.99
Fiber strength NAU934 A05(Chr.05) 186.594 3.82 ns 14.08
NAU1200 A05(Chr.05) 2.68 ns 14.5
NAU474 A07(Chr.07) 30.627 ns 2.43 17.44
NAU1043 A07(Chr.07) ns 2.14 15.86
BNL1317 A09(Chr.09) 88.456 ns 2.37 12.58
NAU2508 A10(Chr.10) 126.827 ns 3.29 23.99
JESPR295 A12(Chr.12) 32.732 2.28 2.72 10.56 14.19
NAU445 A12(Chr.12) 123.764 2.60 ns 11.96
JESPR153 A13(Chr.13) 63.046 5.42 ns 23.3
BNL1395 D07(Chr.16) 78.652 ns 2.12 13.62
BNL1122 D07(Chr.16) 88.995 ns 2.23 14.19
BNL2634 D07(Chr.16) 2.72 ns 12.47
NAU1369 D08(Chr.24) 57.434 2.74 ns 12.13
BNL1521 D08(Chr.24) 73.477 ns 2.34 9.26
JESPR78 D08(Chr.24) 75.81 ns 2.34 9.26
NAU1197 D08(Chr.24) 77.384 ns 2.01 7.76
NAU1336 D08(Chr.24) 78.13 ns 2.34 9.26
NAU1262 D08(Chr.24) 88.599 ns 2.34 9.26
NAU1322 D08(Chr.24) 89.779 ns 2.04 10.82
JESPR127 D08(Chr.24) 97.939 ns 2.34 9.26
NAU780 D08(Chr.24) ns 3.60 18.14
NAU816 D08(Chr.24) 3.70 ns 13.6
TMO06 D09(Chr.23) 109.242 5.51 3.80 23.66 19.23
NAU2443 D13(Chr.18) 62.214 6.13 3.16 22.85 13.02
Fiber fineness NAU934 A05(Chr.05) 186.594 ns 3.074 12.62
BNL1317 A09(Chr.09) 88.456 4.31 2.31 18.74 9.71
NAU1162 A11(Chr.11) 99.954 2.05 ns 11.43
JESPR295 A12(Chr.12) 32.732 2.87 ns 12.95
BNL3436 D06(Chr.25) 105.073 2.24 ns 9.68
TMO06 D09(Chr.23) 109.242 2.85 ns 12.84
NAU1004 D09(Chr.23) 13.67 3.03 ns 13.67

Table 5.

SSR loci significantly (-logP≥2.0) associated with fiber quality traits detected in the and their explained phenotypic variation in two years

Phenotypic effects of each QTL allele for the 33 significantly associated loci were measured, and 21, 24 and 7 favorable alleles for fiber length, strength and fineness were identified, respectively. Phenotypic effects and representative materials for each favorable allele are shown in Table 6. A wide variation of the favorable alleles processed in the 99 accessions was observed, with an average of 14.84 and a range of 7 to 21 (see reference [73] for details). Most of the representative materials were fiber-elite lines collected from China or introduced from the United States, and some of them, such as Yumian 1 and 7235, were introgression lines and contained more than half of the favorable alleles. For example, 7235 was developed from several hybridizations with multiple parents, such as a G. anomalum introgression line, Acala 3080 and PD4381 [85, 86]; Yumian 1 was an introgression line with G. barbadense, G. arboreum, and G. raimondii as putative donors, which is characterized by high lint yield and high fiber strength [24]. These fiber-elite materials had been used for tagging QTLs underlying fiber quality traits in previous family-based linkage mapping studies [13, 17-19, 24-25; 39]. With the genetic information provided by this and previous studies, MAS should be complemented in the future breeding programs.

Traits Favorable allele ai Accessions Representative materials
Fiber length NAU934-4 1.0498 17 I-62434, 7235, I-62478
NAU1037-1 4.5458 1 7235
NAU2354-2 0.3662 10 I-62478, I-62479, PD94045
BNL1317-3 0.8522 21 I-62434, 7235, I-62431
NAU445-3 0.7926 31 I-62434, 7235, I-62431
JESPR295-2 0.8233 31 I-62434, 7235, I-62478
JESPR153-3 0.8457 16 I-62434, I-62431, Yumian1
NAU1200-1 0.5373 49 I-62434, I-62478, I-62431
NAU2272-5 5.8050 1 7235
NAU1102-3 0.4564 22 I-62431, I-62479, I-62429
NAU1302-1 4.5458 1 7235
BNL1521-3 5.8050 1 7235
JESPR78-3 5.8050 1 7235
NAU1336-3 5.8050 1 7235
NAU1262-2 5.8050 1 7235
JESPR127-2 4.5458 1 7235
TMO06-3 1.3109 19 I-62434, 7235, Yumian1
NAU904-3 2.3858 1 GA2001163
NAU2443-2 0.8435 17 I-62434, 7235, I-62431
NAU780-4 4.5458 1 7235
NAU1004-2 0.9346 14 I-62434, 7235, TM-1
Fiber strength NAU934-4 2.6759 17 I-62429, I-62478, PD93019
NAU474-2 1.0231 31 PD94042, I-62478, PD93019
BNL1317-3 2.3136 21 I-62429, 7235, I-62433
NAU2508-1 3.7061 8 7235, PD6992, HS427
NAU445-3 0.8072 31 I-62429, 7235, I-62433
JESPR295-2 2.1342 31 I-62429, I-62478, 7235
JESPR153-3 2.8625 16 I-62429, I-62433, I-62434
NAU1200-1 0.3169 49 I-62429, PD94042, I-62478
NAU1043-2 0.9147 29 I-62478, PD93019, Yuwu19
BNL1395-1 2.2677 12 I-62478, 7235, Yumian1
BNL1122-1 2.2677 12 I-62478, 7235, Yumian1
NAU1369-2 1.4296 15 I-62429, I-62433, Yumian1
BNL1521-3 8.9290 1 7235
JESPR78-3 8.9290 1 7235
NAU1197-3 5.6730 2 Xiangmian13, 7235
NAU1336-3 8.9290 1 7235
NAU1262-2 8.9290 1 7235
NAU1322-2 1.7557 17 I-62429, 7235, I-62433
JESPR127-2 8.9290 1 7235
TMO06-3 3.1629 19 I-62429, 7235, I-62433
NAU2443-2 3.0749 17 I-62429, 7235, Yumian1
BNL2634-3 0.8204 26 I-62429, I-62433, Yumian1
NAU780-1 0.4073 71 I-62429, I-62478, Yumian1
NAU816-3 1.6559 29 I-62429, PD94042, I-62478
Fiber fineness NAU934-4 -0.3064 17 Emian11, Emian6, CRI18
BNL1317-3 -0.3617 21 Jinmian2, Jinmian9, Jimian11
NAU1162-2 -0.2338 13 Sumian5, CRI16, Yu668
JESPR295-3 -0.2148 9 Qiannong465, Cangzhou7315-38, Jin185
BNL3436-3 -0.1132 60 Xiangmian11, Jinmian2, Qiannong465
TMO06-3 -0.3587 19 Shiduan5, Tai8033-2, Sumian5
NAU1004-2 -0.1530 14 Qiannong465, Wanmian73-10, Qinli514

Table 6.

Favorable QTL alleles for fiber qualities, their phenotypic effects (ai) and representative materials. Representative accessions are the top-3 entries for the target trait value of accessions with the corresponding favorable allele

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4. Favorable QTL alleles for FOV race 7 resistance

Fusarium wilt (FW), caused by Fusarium oxysporum f. sp. vasinfectum (FOV), is a widespread disease that causes huge losses in cotton (Gossypium ssp.) production worldwide [87-90]. Once established in soil, FOV can survive in the field for several years as Chlamydia spores, even in the absence of a host, and is nearly impossible to eliminate. Although both chemical controls and cultural practices have been employed in protecting plants from damage, the most effective and efficient control should be provided through host resistance [87, 89]. In the past several years, eight races (race 1–8) of FOV had been indentified worldwide that use both cotton and non-cotton differential hosts [88]. Three FOV races (race 3, 7 and 8) had been found in China; race 7 possesses the highest virulence and is the most widely distributed race [91]. Recently, DNA-based techniques were employed in conjunction with pathogenicity tests to validate these races and to test new isolates, and highly virulent isolates of FOV were identified in Australia [92] and the United States [93-94]. Host resistance to FOV races has been widely evaluated in cotton germplasm under both field nursery and greenhouse conditions, and many highly resistant cotton cultivars and breeding lines have been developed through conventional breeding [89, 95-96]. However, little is known about the mechanism and genetic basis of FOV resistance. Some early classical genetic studies have suggested that the inheritance of FOV resistance in cotton is determined by a single gene [97-99], while other studies have suggested that FOV resistance is controlled by multiple genes [100-102]. Large-scale resistance evaluations in breeding programs are time-consuming and labor-intensive, and it is not easy to obtain the ideal genotype simply through phenotypic selection. The cultivar development process has been slow in the face of the emergence of new, highly virulent FOV isolates [89-90].

To accumulate useful information for understanding the genetic basis of FOV 7 resistance and identify favorable alleles for facilitating future molecular resistance breeding, we performed a marker-trait association mapping to detect QTLs underlying FOV 7 resistance in Upland cotton [72]. The 356-accession panel mentioned above [71] was used for association analysis and favorable allele mining. Meanwhile, a composite cross population (CP) with three parents was developed for linkage mapping. Three Upland cotton cultivars, Xuzhou 142, Yumian 21 and Shang 9901, were chosen as parents according to many years of FOV 7 resistance evaluation. Xuzhou 142, an obsolete cultivar selected from STV 2B in the 1970s with large boll size and high lint percentage that is severely infested by FOV 7, was selected as the susceptible parent. Yumian 21 was released in 1999 and is currently used as a resistant control in national cotton regional trials due to its extremely high resistance to FOV 7. The cultivar Shang 9901 is an anonymous breeding line with high yield potential and moderately high resistance to FOV 7. Thus serial QTLs for FOV 7 resistance were detected by joint population-based association mapping and family-based linkage mapping. Marker–trait association mapping was performed with the MLM model implemented in TASSEL software [82]. A total of 27 markers were significantly associated with FOV 7 resistance at the at the α=0.01 level (-logP=2.0), and were localized to 16 chromosomes (Table 7). Of these loci, 23 were detected under field nursery conditions, 10 were detected in the greenhouse and 6 were detected under both conditions. The proportion of phenotypic variation explained by the markers ranged from 1.48% to 12.99%, with an average of 4.21%. If a more stringent threshold by the Bonferroni correction (P≤0.05/145,-logP≥3.46) is adopted [83], only 10 associations were significant (Table 7). Five (qFW-A3-1, qFW-A12-1, qFW-D3-1, qFW-D5-1 and qFW-D8-1) of the 7 QTLs identified by linkage mapping could be detected by association mapping at the α=0.05 level, while only 3 QTLs (qFW-D3-1, qFW-D5-1 and qFW-D8-1) could be detected at the α=0.01 level (see reference [72] for details).

Marker loci Chr. Position(cM) -Log10P R2(%)
Gh FN Gh FN
NAU2437 A01(Chr.01) 15.248 ns 2.19 5.21
NAU4073 A01(Chr.01) 85.025 2.03 ns 1.48
JESPR304 A02(Chr.02) 22.517 ns 7.09 9.65
NAU934 A05(Chr.05) 19.421 ns 4.61 5.78
NAU3273 A05(Chr.05) 37.244 ns 2.85 2.81
NAU797 A05(Chr.05) 143.032 2.04 ns 3.22
BNL3255 A08(Chr.08) 81.913 ns 3.20 4.41
STV031 A10(Chr.10) 12.751 2.09 2.82 3.34 4.34
NAU440 A10(Chr.10) 42.017 2.80 2.57 2.81 2.51
Gh369 A11(Chr.11) 84.701 ns 2.29 3.61
BNL1066 A11(Chr.11) 134.486 ns 2.12 3.94
BNL1707 A13(Chr.13) 104.694 2.29 ns 1.59
NAU1070 D02(Chr.14) 13.885 2.47 5.89 3.10 7.56
CIR246 D02(Chr.14) 112.473 2.55 3.14 3.65 4.56
BNL3590 D03(Chr.17) 39.284 ns 2.77 3.66
BNL834 D03(Chr.17) 81.098 ns 5.07 5.46
NAU6966 D04(Chr.22) 36.51 ns 2.47 4.16
NAU3095 D05(Chr.19) 10.634 4.20 4.04 5.12 4.99
NAU2816 D05(Chr.19) 160.89 ns 2.62 4.20
BNL3594 D06(Chr.25) 7.66 7.87 4.12 12.99 7.92
NAU3911 D07(Chr.16) 28.658 ns 3.52 3.93
NAU6752 D07(Chr.16) 74.671 ns 3.35 3.44
NAU478 D08(Chr.24) 67.677 2.08 ns 3.86
NAU3207 D08(Chr.24) 90.625 ns 5.21 5.70
NAU1350 D08(Chr.24) 108.529 ns 4.38 4.69
NAU5418 D11(Chr.21) 75.156 ns 3.71 6.98
NAU3084 D12(Chr.26) 0 ns 2.36 2.27

Table 7.

SSR loci significantly (-logP≥2.0) associated with FOV 7 resistance and their explained phenotypic variation in greenhouse and field nursery evaluations. Gh and FN indicate the results evaluated in greenhouse and field nursery, respectively; ns indicates non-significant at α=0.01 level

The phenotypic effects of each QTL allele of the 27 associated loci were estimated in both the greenhouse and field nursery evaluations according to the method mentioned above [71], and therefore, favorable alleles for FOV 7 resistance were identified (Table 8). The phenotypic effects of the 27 loci in the greenhouse and field nursery averaged-1.31 and-3.45, with ranges of-3.81to-0.01 and-14.88 to-0.20, respectively. Among the favorable alleles, NAU934-2 had the most negative phenotypic effect in the greenhouse assay and was able to decrease FW DI by 3.8, while NAU6966-3 had the most negative phenotypic effect in the field nursery and was able to decrease FW DI by 14.88. A wide variation of the favorable alleles processed in the 356 accessions was observed, with an average of 14.84 and a range of 7 to 21 (see [72] for details). Pearson correlation analysis between the number of favorable alleles and FW DI was carried out, and highly negative significance was found in both the greenhouse (r=-0.344, P<0.001) and the field nursery (r=-0.488, P<0.001) evaluations. The top-3 resistant accessions for each favorable allele were listed as representative materials in Table 8.

Favorable alleles ag af Accessions Representative materials
NAU2437-4 -3.27 -8.49 26 Yumian21,Xingmian2,Shiyuan321
NAU4073-2 -1.46 -0.68 172 Xinluzao24,Xingmian2,Xinluzao28
JESPR304-1 -0.45 -2.69 316 Yumian21,Jimian14,Xinluzao24
NAU934-2 -3.81 -4.68 39 Xinluzao24,Zhongmiansuo3,Xinluzao28
NAU3273-1 -0.13 -0.79 341 Yumian21,Jimian14,Xinluzao24
NAU797-2 -1.51 -1.03 172 Yumian21,Jimian14,Xinluzao24
BNL3255-1 -0.01 -2.33 284 Yumian21,Jimian14,Xinluzao24
STV031-1 -2.09 -4.91 137 Yumian21,Xingmian2,Zhongmiansuo3
NAU440-1 -0.33 -0.60 347 Yumian21,Jimian14,Xinluzao24
Gh369-2 -1.02 -3.33 10 Xinluzao24,Xinluzao28,Emian13
BNL1066-4 -1.96 -4.14 15 Zhongmiansuo27,Jimian20,Emian13
BNL1707-2 -1.93 -4.79 112 Yumian21,Xinluzao24,Xingmian2
NAU1070-1 -1.73 -5.84 199 Jimian14,Xinluzao24,Xingmian2
CIR246-3 -2.77 -8.65 16 Ejing1,Ekangmian3,Zhongmiansuo49
BNL3590-1 -0.06 -1.04 315 Yumian21,Jimian14,Xinluzao24
BNL834-1 -0.24 -1.70 312 Yumian21,Jimian14,Xingmian2
NAU6966-3 -1.89 -14.88 37 Xinluzao24,Xinluzao28,GK44
NAU3095-1 -2.30 -4.55 192 Yumian21,Xinluzao24,Xingmian2
NAU2816-2 -1.70 -3.43 152 Xinluzao24,Jimian14,Zhongmiansuo3
BNL3594-1 -2.58 -5.23 201 Jimian14,Xingmian2,Zhongmiansuo9
NAU3911-1 -0.33 -1.91 282 Yumian21,Jimian14,Xingmian2
NAU6752-1 -0.14 -0.83 341 Yumian21,Jimian14,Xinluzao24
NAU478-1 -0.21 -0.20 239 Yumian21,Jimian14,Xingmian2
NAU3207-1 -0.17 -2.27 301 Yumian21,Xinluzao24,Xingmian2
NAU1350-1 -0.03 -0.62 348 Yumian21,Xinluzao24,Jimian14
NAU5418-1 -2.90 -2.44 47 Yumian21,Jimian14,Yumian20
NAU3084-1 -0.42 -1.13 326 Yumian21,Jimian14,Xinluzao24

Table 8.

Favorable QTL alleles, phenotypic effects (ai) and their representative materials. ag and af indicate phenotypic effects evaluated in greenhouse and field nursery conditions, respectively; Representative materials are the top-3 accessions with low disease index

Wang and Roberts identified a major resistance gene (Fov1) against FOV 1 in G. barbadense cv. Pima-S7 and indicated that one or more minor genes in Acala NemX could delay wilt symptoms [54]. Ulloa et al. performed QTL mapping in an F2 (Pima-S7 x Acala NemX) and a recombinant inbred line (RIL; G. hirsutum TM-1 x G. barbadense Pima 3-79) population. The authors detected 6 QTLs (Fov1-C06, Fov1-C08, Fov1-C111, Fov1-C112, Fov1-C16 and Fov1-C19) conferring FOV 1 resistance in different genetic backgrounds [58]. Lopez-Lavalle et al. used an intraspecific cross between the Upland cotton MCU-5 and Siokra 1-4 to detected QTLs conferring resistance to Australian FOV races and found that MCU-5 resistance is complex, with 3 QTLs identified in F3 and 8 ones in F4. The QTLs were localized to chromosomes A6, D4 and D6 [60]. Very recently, Ulloa et al. investigated 3 intraspecific (G. hirsutum x G. hirsutum L. and G. barbadense x G. barbadense L.), 5 interspecific (G. hirsutum x G. barbadense) and one RIL population in 4 greenhouse and 2 field experiments and identified a set of 11 SSR markers across 6 linkage groups/chromosomes (3, 6, 8, 14, 17 and 25) associated with FOV 4 resistance [59]. Integrating information mentioned above with the QTL mapping results for FOV 7 in our study, it suggests that the inheritance of FOV resistance in cotton should be far more complex than has been elucidated to date. Interestingly, the map positions of some QTLs conferring resistance against different FOV races coincided, suggesting that these genes may play the same or similar roles in the process of different host-pathogen and/or environment interactions. While great differences in the resistance of cotton genotypes against FOV races have been demonstrated in many evaluations worldwide, a genotype with a high level of resistance against all FOV races has not been found. Given that FW is the result of interactions among the host, pathogen and environment, many genes must be involved in this process. The accumulation of resistance alleles, even with relatively minor individual effects, will result in higher levels of resistance. The Upland cotton cultivars from China and Africa have shown more resistance to Australian FOV races [90]. Therefore, the favorable alleles and their typical germplasm resources identified in this study should have great potential for developing highly resistant Upland cotton cultivars in future molecular breeding programs.

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5. Discussion and future prospects

Compared to family-based linkage mapping, population-based association mapping offers several advantages such as: (1) the saving of time and money by using existing populations instead of creating cross-controlled populations, (2) analysis of more than two alleles per locus on average, and (3) high expected resolution owing to a short extent of linkage disequilibrium [103]. All these make association mapping an increasingly important tool for complex trait dissection [63, 104].

A suitable association mapping panel should embrace as much phenotypic and genotypic diversity as possible. For example, because the 99-accession panel [73] comprised higher diversity in fiber properties than the 356-accession panel [71] and 81-accession panel [69], both the number of significant associations detected and the proportion of phenotypic variation explained by markers were improved. However, the presence of high diversity from different genetic origins may induce LD between unlinked loci, and consequently result in spurious marker-trait associations [105]. Although several statistical strategies have been developed to account for issues related to population structure and relatedness in order to decrease false positive [106-107], whether these approaches will induce new false negative remain unclear. Synthetic association populations, such as the multi-parent advanced generation inter-cross (MAGIC) population [108] and the nested association mapping (NAM) population in maize [109], had been used for genome-wide association studies (GWAS); with which both high resolution and better population structure control can be achieved [110-111]. It is certain that such populations had been or are being developed in Upland cotton. Two artificially controlled multiple parent random-mating populations with each comprising more than 800 lines had been developed in our library, which should provide another theoretically ideal panel for association mapping.

The other inherent constraint limiting the successful use of association mapping is rare alleles exiting in natural populations. Given that the number of individuals with a specific genotype is quite small, the effect of rare alleles on mapping can go far beyond the effect of small population sizes [112]. Large population size had been considered as an important factor to improve the QTL detection power in association mapping studies [3, 112]. Many more marker-trait associations for fiber yield were detected in our 356-accession panel [71] than in the 81-accession panel [69] between the same markers and target traits at the same significance level. While family–based linkage mapping can make use of alleles that occur at low frequencies in natural populations by designing crosses to create artificial populations with inflated frequencies of those alleles. So specifically designed mapping populations such as recombinant inbred lines (RIL) and near isogenic lines (NIL) will remain important. Furthermore, joint linkage and association mapping was recommended as an alternative approach to overcome some of the inherent limitations of both linkage and association mapping [105, 112], and this approach has proven to be a powerful tool to detecting architecture of complex traits [72, 113-118].

Recently, two preliminary maps of the whole-genome scaffolds of G. raimondii (the putative diploid donor for tetraploid species) were separately released by two different groups [119-120], which will facilitate the tetraploid genome sequencing and assembly. Real genome-wide association mapping will be realized in the near future through resequencing or other high-throughput genotyping technologies [121], which will dramatically accelerate genetic diversity exploitation and favorable allele mining in Upland cotton germplasm resources.

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Acknowledgments

We thank all faculties and graduate students in Cotton Research Institute, Nanjing Agricultural University and the other members of the cotton genomics and breeding communities for their contributions, and apologize for not citing many enlightening papers owing to space limitations. This study was supported by grants from 973 (2011CB109300), 863, Jiangsu Province Key Project (BE2012329), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

References

  1. 1. National Cotton Council, USA. http://www.cotton.org (accessed 10 October 2013).
  2. 2. Zhang HB, Li YN, Wang BH, Chee PW. Recent advances in cotton genomics. Int J Plant Genomics 2008; ID 742304. doi: 10.1155/2008/742304.
  3. 3. Mackay TFC, Stone EA, Ayroles JF. The genetics of quantitative traits: challenges and prospects. Nat Rev Genet 2009; 10: 565–577.
  4. 4. Xu Y, Crouch J. Marker-assisted selection in plant breeding: from publications to practice. Crop Sci 2008; 48: 391–407.
  5. 5. Jannink JL, Lorenz AJ, Iwata H. Genomic selection in plant breeding: from theory to practice. Brief Funct Genomics 2010; 9: 166–177.
  6. 6. Jiang CX, Writght RJ, EL-Zik KM, et al. Polyploid formation created unique avenues for response to selection in Gossypium (cotton). Proc Natl Acad Sci USA 1998; 95: 4419–4424.
  7. 7. Ulloa M, Meredith WR. Genetic linkage map and QTL analysis of agronomic and fiber quality traits in an intraspecific population. J Cotton Sci 2000; 4: 161–170.
  8. 8. Ulloa M, Saha S, Jenkin N, et al. Chromosomal assignment of RFLP linkage groups harboring important QTL on an intraspecific cotton (Gossypium hirsutum L.) joinmap. J Hered 2005; 96: 132–144.
  9. 9. Kohel RJ, Yu J, Park YH, et al. Molecular mapping and characterization of traits controlling fiber quality in cotton. Euphytica 2001; 121: 163–172.
  10. 10. Saranga Y, Menz M, Jiang CX, et al. Genomic dissection of genotype x environment interactions conferring adaptation of cotton to arid conditions. Genome Res 2001; 11: 1988–1995.
  11. 11. Ren LH, Guo WZ, Zhang TZ. Identification of quantitative trait loci (QTLs) affecting yield and fiber properties in chromosome 16 in cotton using substitution line. Acta Botanica Sinica 2002; 44: 815–820.
  12. 12. Paterson AH, Saranga Y, Menz M, et al. QTL analysis of genotype x environment interaction affecting cotton fiber quality. Theor Appl Genet 2003; 6: 384–396.
  13. 13. Zhang T, Yuan Y, Yu J, et al. Molecular tagging of a major QTL for fiber strength in Upland cotton and its marker-assisted selection. Theor Appl Genet 2003; 106: 262–268.
  14. 14. Wu MQ, Zhang XL, Nie YC, et al. Localization of QTLs for yield and fiber quality traits of tetraploid cotton cultivar. Acta Genetica Sinica 2003; 30: 443–452.
  15. 15. Mei M, Syed NH, Gao W, et al. Genetic mapping and QTL analysis of fiber related traits in cotton (Gossypium). Theor Appl Genet 2004; 108: 280–291.
  16. 16. Lacape JM, Nguyen TB, Courtois B, et al. QTL analysis of cotton fiber quality using multiple Gossypium hirsutum x Gossypium barbadense backcross generations. Crop Sci 2005; 45: 123–140.
  17. 17. Shen X, Guo W, Zhu X, et al. Molecular mapping of QTLs for fiber qualities in three diverse lines in Upland cotton using SSR markers. Mol Breed 2005; 15: 169–181.
  18. 18. Shen X, Zhang T, Guo W, et al. Mapping fiber and yield QTLs with main, epistatic, and QTL x Environment interaction effects in recombinant inbred lines of Upland cotton. Crop Sci 2006; 46: 61–66.
  19. 19. Shen X, Guo W, Lu Q, et al. Genetic mapping of quantitative trait loci for fiber quality and yield trait by RIL approach in Upland cotton. Euphytica 2007; 155: 371–380
  20. 20. Chee P, Draye X, Jiang CX, et al. Molecular dissection of interspecific variation between Gossypium hirsutum and Gossypium barbadense (cotton) by a backcross-self approach: I. Fiber elongation. Theor Appl Genet 2005; 111: 757–763.
  21. 21. Chee P, Draye X, Jiang CX, et al. Molecular dissection of phenotypic variation between Gossypium hirsutum and Gossypium barbadense (cotton) by a backcross-self approach: III. Fiber length. Theor Appl Genet 2005; 111: 772–781.
  22. 22. Park YH, Alabady MS, Sickler B, et al. Genetic mapping of new cotton fiber loci using EST-derived microsatellites in an interspecific recombinant inbred line (RIL) cotton population. Mol Genet Genom 2005; 274: 428–441.
  23. 23. Lin ZX, He DH, Zhang XL, et al. Linkage map construction and mapping QTL for cotton fibre quality using SRAP, SSR and RAPD. Plant Breed 2005; 124: 180–187.
  24. 24. Zhang ZS, Xiao Y, Luo M, et al. Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.). Euphytica 2005; 144: 91–99.
  25. 25. Zhang ZS, Hu MC, Zhang J, et al. Construction of a comprehensive PCR-based marker linkage map and QTL mapping for fiber quality traits in upland cotton (Gossypium hirsutum L.). Mol Breed 2009; 24: 49–61.
  26. 26. Draye X, Chee P, Jiang CX, et al. Molecular dissection of interspecific variation between Gossypium hirsutum and G. barbadense (cotton) by a backcross-self approach: II. Fiber fineness. Theor Appl Genet 2005; 111: 764–771.
  27. 27. Saha S, Jenkins JN, Wu J, et al. Effects of chromosome specific introgression in upland cotton on fiber and agronomic traits. Genetics 2006; 172: 1927–1938.
  28. 28. Wang B, Guo W, Zhu X, et al. QTL mapping of fiber quality in an elite hybrid derived-RIL population of upland cotton. Euphytica 2006; 152: 367–378.
  29. 29. Frelichowski JE, Palmer M, Main D, et al. Genetic mapping of microsatellites derived from BAC-end sequences of Gossypium hirsutum Acala ‘Maxxa’ BAC-ends. Mol Genet Genom 2006; 275: 479–491.
  30. 30. He DH, Lin ZX, Zhang XL, et al. QTL mapping for economic traits based on a dense genetic map of cotton with PCR-based markers using the interspecific cross of Gossypium hirsutum x Gossypium barbadense. Euphytica 2007; 153: 181–197.
  31. 31. Qin H, Guo W, Zhang Y, et al. QTL mapping of yield and fiber traits based on a four-way cross population in Gossypium hirsutum L. Theor Appl Genet 2008; 117: 883–894.
  32. 32. Wu JX, Gutierrez OA, Jenkins JN, et al. Quantitative analysis and QTL mapping for agronomic and fiber traits in an RI population of upland cotton. Euphytica 2009; 165: 231–245.
  33. 33. Chen H, Qian N, Guo W, et al. Using three overlapped RILs to dissect genetically clustered QTL for fiber strength on Chro.D8 in Upland cotton. Theor Appl Genet 2009; 119: 605–12.
  34. 34. Luan M, Guo X, Zhang Y, et al. QTL mapping for agronomic and fiber traits using two interspecific chromosome substitution lines of Upland cotton. Plant Breed 2009; 128: 671–679.
  35. 35. Qin YS, Ye WX, Liu RZ, et al. QTL mapping for fiber quality properties in upland cotton (Gossypium hirsutum L.). Sci Agric Sin 2009; 42: 4145–4154.
  36. 36. An CF, Jenkins JN, Wu JX, et al. Use of fiber and fuzz mutants to detect QTL for yield components, seed, and fiber traits of upland cotton. Euphytica 2010; 172: 21–34.
  37. 37. Wang FR, Gong YC, Zhang CY, et al. Genetic effects of introgression genomic components from sea island cotton (Gossypium barbadense L.) on fiber related traits in upland cotton (G. hirsutum L.). Euphytica 2011; 181: 41–53.
  38. 38. Sun FD, Zhang JH, Wang SF, et al. QTL mapping for fiber quality traits across multiple generations and environments in upland cotton. Mol Breed 2012; 30: 569–582.
  39. 39. Zhang K, Zhang J, Ma J, et al. Genetic mapping and quantitative trait locus analysis of fiber quality traits using a three-parent composite population in upland cotton (Gossypium hirsutum L.). Mol Breed 2012; 29: 335–348
  40. 40. Ning Z, Chen H, Mei H, et al. Molecular tagging of QTLs for fiber quality and yield in the upland cotton cultivar Acala-Prema. Euphytica 2014; 195: 143–156.
  41. 41. He DH, Lin ZX, Zhang XL, et al. Mapping QTLs of traits contributing to yield and analysis of genetic effects in tetraploid cotton. Euphytica 2005; 144: 141–149.
  42. 42. Wang B, Guo W, Zhu X, et al. QTL mapping of yield and yield components for elite hybrid derived-RILs in upland cotton. J Genet Genomics 2007; 34: 35–45.
  43. 43. Qin YS, Liu RZ, Mei HX, et al. QTL Mapping for yield traits in Upland cotton (Gossypium hirsutum L.). Acta Agronomica Sinica 2009; 35: 1812–1821.
  44. 44. Chen H, Qian N, Guo WZ, et al. Using three selected overlapping RILs to fine-map the yield component QTL on Chro.D8 in Upland cotton. Euphytica 2010; 176: 321–329.
  45. 45. Liu R, Wang B, Guo W, et al. Quantitative trait loci mapping for yield and its components by using two immortalized populations of a heterotic hybrid in Gossypium hirsutum L. Mol Breed 2012; 29: 297–311.
  46. 46. Shen X, Becelaere GV, Kumar P, et al. QTL mapping for resistance to root-knot nematodes in the M-120 RNR Upland cotton line (Gossypium hirsutum L.) of the Auburn 623 RNR source. Theor Appl Genet 2006; 113: 1539–1549.
  47. 47. Wang C, Ulloa M, Roberts PA. Identification and mapping of microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in Acala NemX cotton (Gossypium hirsutum L.). Theor Appl Genet 2006; 112: 770–777.
  48. 48. Ynturi P, Jenkins JN, McCarty Jr JC, et al. Association of root-knot nematode resistance genes with simple sequence repeat markers on two chromosomes in cotton. Crop Sci 2006; 46: 2670–2674.
  49. 49. Romano GB, Sacks EJ, Stetina SR, et al. Identification and genomic location of a reniform nematode (Rotylenchulus reniformis) resistance locus (Renari) introgressed from Gossypium aridum into upland cotton (G. hirsutum).Theor Appl Genet 2009; 120: 139–150.
  50. 50. Bolek Y, El-Zik Km, Pepper AE, et al. Mapping of Verticillium wilt resistance genes in cotton. Plant Sci 2005; 168: 1581–1590.
  51. 51. Wang HM, Lin ZX, Zhang XL, et al. Mapping and quantitative trait loci analysis of verticillium wilt resistance genes in cotton. J Integr Plant Biol 2008; 50: 174–182.
  52. 52. Jiang F, Zhao J, Zhou L, et al. Molecular mapping of Verticillium wilt resistance QTL clustered on chromosomes D7 and D9 in upland cotton. Sci China C Life Sci 2009; 52: 872–884.
  53. 53. Ning Z, Zhao R, Chen H, et al. Molecular tagging of a major quantitative trait locus for broad-spectrum resistance to Verticillium wilt in upland cotton cultivar Prema. Crop Sci 2013; 53: 1–9.
  54. 54. Wang C, Roberts PA. A Fusarium wilt resistance gene in Gossypium barbadense and its effect on root-knot nematode-wilt disease complex. Phytopathology 2006; 96: 727–734.
  55. 55. Chen X, Ge F, Wang D, et al. Mapping of QTLs conferring resistance to Fusarium wilt in cotton. Molecular Plant Breeding 2008; 6: 1127–1133.
  56. 56. Wang P, Su L, Qin L, et al. Identification and molecular mapping of a Fusarium wilt resistant gene in upland cotton. Theor Appl Genet 2009; 119: 733–739.
  57. 57. Wang P, Shi L, Su L, et al. Quantitative trait loci for resistance against Fusarium wilt based on three cotton F2 populations. Agricultural Sciences in China 2010; 9: 1799–1806.
  58. 58. Ulloa M, Wang C, Hutmacher RB, et al. Mapping Fusarium wilt race 1 resistance genes in cotton by inheritance, QTL and sequencing composition. Mol Genet Genomics 2011; 286: 21–36.
  59. 59. Ulloa M, Hutmacher RB, Roberts PA, et al. Inheritance and QTL mapping of Fusarium wilt race 4 resistance in cotton. Theor Appl Genet 2013; 126: 1405–1418.
  60. 60. Lopez-Lavalle LAB, Gillespie VJ, Tate WA, et al. Molecular mapping of a new source of Fusarium wilt resistance in tetraploid cotton (Gossypium hirsutum L.). Mol Breed 2012; 30: 1181–1191.
  61. 61. Rong J, Feltus FA, Waghmare VN, et al. Meta-analysis of polyploid cotton QTL shows unequal contributions of subgenomes to a complex network of genes and gene clusters implicated in lint fiber development. Genetics 2007; 176: 2577–2588.
  62. 62. Lacape JM, Llewellyn D, Jacobs J, et al. (2010) Meta-analysis of cotton fiber quality QTLs across diverse environments in a Gossypium hirsutum x G. barbadense RIL population. BMC Plant Biol 2010; 10: 132.
  63. 63. Zhu C, Gore M, Buckler ES, et al. Status and prospects of association mapping in plants. Plant Genome 2008; 1: 5–20.
  64. 64. Kantartzi SK, Stewart JMcD. Association analysis of fibre traits in Gossypium arboreum accessions. Plant Breed 2008; 127: 173–179.
  65. 65. Abdurakhmonov IY, Kohel RJ, Yu JZ, et al. Molecular diversity and association mapping of fiber quality traits in exotic G. hirsutum L. germplasm. Genomics 2008; 92: 478–487.
  66. 66. Abdurakhmonov IY, Saha S, Jenkins JN, et al. Linkage disequilibrium based association mapping of fiber quality traits in G. hirsutum L. variety germplasm. Genetica 2009; 136: 401–417.
  67. 67. Zeng L, Meredith Jr WR, Gutierrez OA, et al. Identification of associations between SSR markers and fibre traits in an exotic germplasm derived from multiple cross among Gossypium tetraploid species. Theor Appl Genet 2009; 119: 93–103.
  68. 68. Rakshit A, Rakshit S, Singh J, et al. Association of AFLP and SSR markers with agronomic and fibre quality traits in Gossypium hirsutum L. J Genetics 2010; 89: 155–162.
  69. 69. Zhang T, Qian N, Zhu X, et al. Variations and transmission of QTL alleles for yield and fiber qualities in Upland cotton cultivars developed in China. PLoS ONE 2013; 8: e57220.
  70. 70. Wang XQ, Yu Y, Li W, et al. Association analysis of yield and fiber quality traits in Gossypium barbadense with SSRs and SRAPs. Genet Mol Res 2013; 12: 3353–3362.
  71. 71. Mei H, Zhu X, Zhang T. Favorable QTL alleles for Yield and Its Components Identified by Association Mapping in Chinese Upland Cotton Cultivars. PLOS ONE 2013; 8: e82193.
  72. 72. Mei H, Ai N, Zhang X, et al. QTLs conferring FOV 7 resistance detected by linkage and association mapping in Upland cotton. Euphytica 2014; Doi 10.1007/s10681-014-1063-y.
  73. 73. Cai C, Ye W, Zhang T, et al. Association analysis of fiber quality traits and exploration of elite alleles in Upland cotton cultivars/accessions (Gossypium hirsutum L.). J Integr Plant Biol 2014; 56: 51–62.
  74. 74. Breseghello F, Sorrells ME. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics 2006; 172: 1165–1177.
  75. 75. Jia L, Yan W, Zhu C, et al. Allelic analysis of sheath blight resistance with association mapping in rice. PLoS ONE 2012; 7: e32703.
  76. 76. Huang ZK., editor. The Cultivars and Their Pedigree of Cotton in China. China Agriculture Press, Beijing; 2007.
  77. 77. Du X, Sun J, Zhou Z, et al. Current situation and the future in collection, preservation, evaluation and utilization of cotton germplasm in China. J Plant Genetic Resources 2012; 13: 163–168.
  78. 78. Guo W, Cai C, Wang C, et al. A microsatellite-based, gene-rich linkage map reveals genome structure, function and evolution in Gossypium. Genetics 2007; 176: 527–541.
  79. 79. Pritchard JK, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics 2000; 155: 945–959.
  80. 80. Evanno G, Regnaut S, Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 2005; 14: 2611–2620.
  81. 81. Hardy O, Vekemans X. SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes 2002; 2: 618–620.
  82. 82. Bradbury PJ, Zhang Z, Kroon DE, et al. TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 2007; 23: 2633–2635.
  83. 83. Lander ES, Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 1989; 121: 185–199.
  84. 84. Wang Y, Yang W, Xu H, et al. The main problems and recommendations in Chinese cotton production. Chin Agr Sci Bull 2009; 25: 86–90.
  85. 85. Qian S, Huang J, Peng Y, et al. Studies on the hybrid of G. hirsutum L. and G. anomalum Wawr. & Peyr. and application in breeding. Sci Agric Sin 1992; 25: 44–51.
  86. 86. Zhou B, Zhu X, Guo W, et al. Major gene plus poly-gene inheritance of super quality fiber properties in upland cotton introgressed from Gossypium anomalum Wawra & Peyritsch. Cotton Sci 2006; 18: 60–62.
  87. 87. Ma C., editor. Researches on Fusarium and Verticillium wilt in cotton. China Agriculture Press, Beijing; 2007.
  88. 88. Davis RM, Colyer PD, Rothrock CS, et al. Fusarium wilt of cotton: population diversity and implications for management. Plant Dis 2006; 90: 692–703.
  89. 89. Ulloa M, Hutmacher RB, Davis RM, et al. Breeding for Fusarium wilt race 4 resistance in cotton under field and greenhouse conditions. J Cotton Sci 2006; 10: 114–127.
  90. 90. Constable GA, Reid PE, Stiller WN. Breeding for resistance to a new strain of Fusarium wilt in Australia. In: Stephens M. (eds.) Proceedings of the World Cotton Research Conference-4, Sept 10-14, Lubbock TX; 2007.
  91. 91. Chen Q, Ji X, Sun W. Identification of cotton-wilt Fusarium races in China. Scientia Agricultura Sinica 1985; 6: 1–6.
  92. 92. Wang B, Brubaker CL, Burdon JJ. Fusarium species and Fusarium wilt pathogens associated with native Gossypium populations in Australia. Mycol Res 2004; 108: 35–44.
  93. 93. Kim Y, Hutmacher RB, Davis RM. Characterization of California isolates of Fusarium oxysporum f. sp. vasinfectum. Plant Dis 2005; 89: 366–372.
  94. 94. Holmes EA, Bennett RS, Spurgeon DW, et al. New genotypes of Fusarium oxysporum f. sp. vasinfectum from the Southeastern United States. Plant Dis 2009; 93: 1298–1304.
  95. 95. Ma C, Jian G, Zheng C. The advances in cotton breeding resistance to Fusarium and Verticillium wilts in China during past fifty years. Scientia Agricultura Sinica 2002; 35: 508–513.
  96. 96. Ulloa M, Percy R, Zhang J, et al. Registration of four Pima cotton germplasm lines having good levels of Fusarium wilt race 4 resistance with moderate yields and good fibers. J Plant Registr 2009; 3: 198–202.
  97. 97. Smith AL, Dick JB. Inheritance of resistance to Fusarium wilt in upland cotton and sea island cotton as complicated by nematodes under field conditions. Phytopathology 1960; 50: 44–48.
  98. 98. Netzer D, Tal Y, Marani A, et al. Resistance of interspecific cotton hybrids (Gossypium hirsutum x Gossypium barbadense containing Gossypium harknessii cytoplasm) to fusarium wilt. Plant Dis 1985; 69: 312–313.
  99. 99. Feng C, Zhang J, Liu J, et al. Inheritance of resistance to Fusarium wilt in several Chinese upland cotton varieties. Acta Agronomica Sinica 1996; 22: 550–554.
  100. 100. Kappelman AJ. Inheritance of resistance to Fusarium wilt in cotton. Crop Sci 1971; 11: 672–674.
  101. 101. Jiao B. Primary research of general combining ability and heritability of resistance to Fusarium wilt and Verticillium wilt in upland cotton. Acta Agronomica Sinica 1985; 11, 267–273.
  102. 102. Wang Z, Ma Z, Qu J. Analysis of gene effect of resistance to Fusarium and Verticillum wilt in upland cotton. Journal of Heibei Agriculture University 1989; 12: 21–25.
  103. 103. Flint-Garcia SA, Thuillet AC, Yu J, et al. Maize association population: a high-resolution platform for quantitative trait locus dissection. Plant J 2005; 44: 1054–1064.
  104. 104. Abdurakhmonov IY, Abdukarimov A. Application of association mapping to understanding the genetic diversity of plant germplasm resources. Int J Plant Genomics 2008; ID 574927. doi:10.1155/2008/574927.
  105. 105. Gupta PK, Rustgi S, Kulwal PL. Linkage disequilibrium and association studies in higher plants: present status and future prospects. Plant Mol Biol 2005; 57: 461–485.
  106. 106. Price A, Patterson N, Plenge R, et al. Principal components analysis corrects for stratification in genome-wide association studies. Nat Genet 2006; 38: 904–909.
  107. 107. Yu J, Pressoir G, Briggs WH, et al. A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. Nat Genet 2006; 38: 203–208.
  108. 108. Cavanagh C, Morell M, Mackay I, et al. From mutations to MAGIC: resource for gene discovery, validation and delivery in crop plants. Cur Opin Plant Bio 2008; 11: 215–221.
  109. 109. Yu J, Holland JB, McMullen MD, et al. Genetic design and statistical power of nested association mapping in maize. Genetics 2008; 178: 539–551.
  110. 110. Buckler ES, Holland JB, Bradbury PJ, et al. The genetic architecture of maize flowering time. Science 2009; 325: 714–718.
  111. 111. Kover PX, Valdar W, Trakalo J, et al. A multiparent advanced generation inter-cross to fine-map quantitative traits in Arabidopsis thaliana. PLoS Genetics 2009; 5: e1000551.
  112. 112. Myles S, Peiffer J, Brown PJ, et al. Association mapping: critical consideration shift from genotype to experimental design. Plant Cell 2009; 21: 2194–2202.
  113. 113. Nemri A, Atwell S, Tarone AM, et al. Genome-wide survey of Arabidopsis natural variation in downy mildew resistance using combined association and linkage mapping. Proc Natl Acad Sci USA 2010; 107: 10302–10307.
  114. 114. Lu Y, Zhang S, Shah T, et al. Joint linkage-linkage disequilibrium mapping is a powerful approach to detecting quantitative trait loci underlying drought tolerance in maize. Proc Natl Acad Sci USA 2010; 107: 19585–19590.
  115. 115. Brachi B, Faure N, Horton M, et al. Linkage and association mapping of Arabidopsis thaliana flowering time in nature. PLoS Genet 2010; 6: e1000940.
  116. 116. Pagny G, Paulstephenraj PS, Poque S, et al. Family-based linkage and association mapping reveals novel genes affecting Plum pox virus infection in Arabidopsis thaliana. New Phytologist 2012; 196: 873–886.
  117. 117. Korir PC, Zhang J, Wu K, et al. (2013) Association mapping combined with linkage analysis for aluminum tolerance among soybean cultivars released in Yellow and Changjiang River Valleys in China. Theor Appl Genet 2013; doi 10.1007/s00122-013-2082-0.
  118. 118. Cadic E, Coque M, Vear F, et al. Combined linkage and association mapping of flowering time in Sunflower (Helianthus annuus L.). Theor Appl Genet 2013; doi 10.1007/s00122-013-2056-2.
  119. 119. Paterson AH, Wendel JF, Gundlach H, et al. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature 2012; 492: 423–427.
  120. 120. Wang K, Wang Z, Li F, et al. The draft genome of a diploid cotton Gossypium raimondii. Nat Genet 2012; 44: 1098–1103.
  121. 121. Davey JW, Hohenlohe PA, Etter PD, et al. Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nat Rev Genet 2011; 12: 499–510.

Written By

Hongxian Mei, Xiefei Zhu, Wangzhen Guo, Caiping Cai and Tianzhen Zhang

Submitted: 18 February 2014 Published: 02 July 2014

© 2014 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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