1. Introduction
1.1. Status of uranium use and pollution
The energy sector around the world is severely affected by the combined impact of population growth, industrialisation and the general movement of people into cities (urbanisation). Controlling carbon emissions from the energy sector lies at the centre of the strategy to curb the problem of global warming and its effects on changing weather patterns – which poses a threat to ecosystems and biodiversity. Several alternatives are under consideration worldwide to reverse the current trend of global warming; almost all strategies are concern in finding cleaner primary energy sources with low or no carbon emissions. The alternative energy sources include hydropower, wind power, nuclear power, solar energy, biomass energy, and geothermal energy. Among the proposed cleaner energy sources, nuclear energy has been demonstrated to be the most stable and concentrated enough to replace fossil fuels such as coal and natural gas on most national grids.
However, in spite of holding the promise of cleaner production in terms of carbon emissions, nuclear technology produces a waste which is highly radioactive and in most instances difficult to treat. The waste compounds originating from nuclear power generation include uranium and its fission products, and transuranic elements. These metallic elements account for over 95% of the total radioactivity of radioactive waste [1].
The lightweight fission products emanating from nuclear fuel processing plants and underground nuclear waste repositories contain high levels of mobile species such as caesium (Cs-137), strontium (Sr-90), and cobalt (Co-60). These elements are characterised by very high radiological decay rates and short half-lives. Due to their high decay rates and high radioactivity, fission products render the waste matrix in which they are detected highly radiotoxic and therefore hazardous [2]. Some of these elements are easily taken up by plants and other animal life forms upon reaching the environment [3]. As an example, the divalent cation strontium-90 (90Sr2+) is easily incorporated into bone tissue because its chemical properties resemble calcium (Ca2+) which is a critical component of the mammalian bone structure. Calcium is responsible for the bone’s structural integrity and strength and is therefore an essential component of the mammalian diet.
Currently, there are about 438 nuclear power plants in operation in 31 countries around the world providing about 14% of the world’s primary energy needs [4]. The slow progression towards wider application of nuclear energy technology worldwide is mainly due to concerns over long term radiation contamination, reactor accidents such as those which occurred in Chernobyl and Fukushima, and the possibility of proliferation of atomic bomb making materials to renegade regimes and terrorists.
Nuclear power generation is not the only source of potential radioactive pollution. Other activities such as nuclear weapon testing, radioisotope manufacturing, and biomedical research have also contributed significant amounts of radioactive waste into the environment [5]. However, most radioactive waste originating from medical and radioisotope manufacturing facilities is predominantly organic and therefore can be easily degraded [6]. Radioactive waste from the power generation industry which is identified as High Level Waste (HLW), on the other hand, consists of a higher proportion of non-degradable metallic elements such as uranium and fission products, and transuranic elements. The waste tends to be “hot” (highly radiotoxic) to living organisms and therefore requires pre-treatment before disposal.
This chapter presents recent findings from research aimed at developing environmentally friendly treatment processes for radioactive waste. Attempts have recently been made to treat components of high level radioactive waste (HLW) prior disposal in specifically engineered facilities by immobilizing and extracting the radioactive elements in the waste using a combination of biological reduction and biosorption of the toxic metallic elements. In the case of U(VI), the reduced element is easily extracted either by precipitation/deposition on cell surfaces or removed by a biologically mitigated ion exchange process using live cells of bacteria.
1.2. Uranium compound toxicity
Uranium is found in the environment in many forms including as an oxide, organic or inorganic complex, and rarely as a free metallic ion. Free elemental uranium primarily exists in higher oxidation states typically bound to oxygen. In the aqueous phase, cationic uranium readily combines with oxygen to form oxy-cations of uranium (uranyl ions) which are highly mobile and highly reactive. For example U(VI) in the form of (UO22+) is highly soluble in water. Whereas, the reduced form U(IV), existing as uraninite (UO2) is less soluble and therefore represents a lower risk in the environment.
The toxicity of uranium compounds is closely related to its mobility. That is, the most soluble of the uranium species are associated with acute toxicity in organisms [7]. The highly soluble uranium compounds such as UF6, UO2 (NO3)2, UO2Cl2, uranyl acetate, uranyl sulphates, and uranyl carbonates exhibit high toxicity to mammalian cells whereas the less soluble uranium compounds including UO2, U3O8, uranium hydrides, and carbides are less reactive and less toxic.
The permissible body level for soluble compounds is based on chemical toxicity, while the permissible body level for insoluble compounds is based on radiotoxicity. Because all uranium isotopes (U234, U235, U238) mainly emit α-particles that have little penetrating ability, the main radiation hazard from soluble uranium compounds occurs when uranium compounds are ingested or inhaled [7]. Although, absorption of some soluble compounds through skin is possible, uptake through the skin is normally superseded by either surface damage due to exposure or accumulation to toxic levels through other routes of entry such as inhalation and ingestion.
2. Treatment and reclamation of uranium
2.1. Physical-chemical treatment
The most common treatment strategy for uranium and radioactive waste involves the extraction of the radioactive component to reduce the volume of radioactive waste followed by treatment of the bulky nonradioactive waste using conventional methods [8]. Various options have been utilised to achieve extraction by employing a combination of physical-chemical and biological methods. For areas that have already been contaminated, further migration of the pollutants is prevented by using
2.2. Adsorptive/ion exchange processes
Separation processes have been utilised to selectively remove cationic species from wastewater streams. Materials such as activated carbon, saw dust, and peat can remove pollutants from water. However, these materials are not selective and therefore may not be effective in removing metallic elements from nuclear waste. Specially designed resins can be utilised to target specific species by manipulating the composition of functional groups on surface of the resin. Several examples of uranium binding ion exchange systems are reported by several researchers [13-15]. Although proven successful on pilot scale, full implementation of ion exchange uranium separation is hindered by high cost. Additionally, the ion exchange resin surfaces are not self-regenerating, and therefore have limited capacity [13].
2.3. Membrane processes
Conventional membrane systems used in treating uranium includes, nano-pore filtration, ultrafiltration, microfiltration and reverse osmosis [16]. Nano-pore membrane filters have the potential to be used in recovery of radioisotopes from water or gas streams. Membrane technology is now regarded as established technology with predictable and reliable processing capability than most current alternatives. Membranes have become relatively cheap such that there use is no longer regarded uneconomical. In spite of being economically viable membrane processes generate large quantities of used membranes which contribute to the problem of radioactive solid waste from the nuclear industry.
2.4. Chemical extraction
Chemical extraction processes have mostly been used for remediation processes mostly on land. For example, uranium can be extracted from contaminated soil using sodium carbonate/bicarbonate or citric acid [17-19]. Although this process effectively removes uranium from soil, it requires a careful balance during application since overloading the system with the acid agent may further migrate uranium in the environment [18, 20]. Certain chemical agents may oxidise other potentially toxic metals posing further risk to the environment. Furthermore, long-term stability of reaction products is of concern. Changing chemical conditions in future could remobilise the metal to its toxic form.
2.5. Biological treatment processes
Biological methods have been proposed to improve or substitute the conventional physico-chemical methods for the remediation of contaminated environments. Unlike organic compounds, toxic metals cannot be degraded or destroyed but can only be transformed from high oxidation state to lower oxidation state. Microbes can potentially affect the physical and the chemical state of the uranium by altering its speciation, solubility, and sorption properties. Strategies suggested for the removal of metals and radionuclides using appropriate microbes include biosorption, bioaccumulation, bioprecipitation, and bioreduction [21-23].
Biological treatment is based on the prospect of utilising processes already devised by nature in dealing with environmental hazards. During three and a half billion years of evolution, microorganisms have evolved mechanisms to survive in hostile environments and to adapt to changes in the environment [24]. One of the most conserved mechanisms in the living cell is the biochemical pathway for electron-transport through the cytoplasmic membrane to conserve energy through the oxidation of an electron donor and reduction of an electron acceptor such as oxygen. This process has been conserved over billions of years, such that, to this day, all life on earth depends on variants of this pathway [24-26]. Most biochemical processes for degradation and/or detoxification of compounds in the living cell are linked to the above process.
Environmental engineers around the world have undertaken to find ways to tap into the mysteries of nature by diligently studying the action of microorganisms as they adapt to extreme conditions. Lately, microorganisms have been isolated that are capable of reducing the toxic forms of heavy metal and transitional metal elements in transuranic waste (TRU) to less mobile precipitable forms [27]. Other researchers have found microbial cultures with the capability to resist high radiation doses.
Most of the microorganisms discovered thus far, utilise the energy derived from change in the redox potential or oxidation states of the compounds for metabolic purposes. No organism has yet been shown to utilise the energy derived from radioactive decay for metabolism. But recent research show promise that this scenario is soon to change. For example, recent studies have shown that microorganism may not only resist radiation, but may to a certain degree utilize the radiation for metabolic advantage. One example was illustrated in a recent study in which cultures of melanising fungi from the cold regions utilized ionizing radiation to derive metabolic energy [28].
2.6. Biosorption
Biosorption is used to describe the metabolism independent sorption (passive process). In this process uranium-bearing water is brought into contact with either living or dead biomass that possesses abandoned functional groups (carboxyl, hydroxyl, amine, and phosphate group) within the surface layer. The charged group within the cell surface layer is able to interact with ions or charged molecules present in the uranium-bearing water. As a result metal cations become electrostatically attracted and bound to the cell surface layer.
Polymers secreted by many metabolizing microbes can also immobilize metals. Different studies on biosorption demonstrated that uranium biosorption is reversible, species-specific, and depends upon the chemistry and pH of the solution, physiological state of cells as well as the presence of the extracellular soluble polymers (EPS) [29, 30]. In this process desorption and recovery of heavy metals and radionuclides for further reuse is easy [31]. Biosorption of radionuclides to the cell surface and polymer substance is a promising technology for remediating contaminated waters. However, the effectiveness of this process is highly affected by pH of the solution and saturation of the biosorbent when metal interactive sites are occupied. Studies were done [32, 33] to investigate the biosorption of uranium under acid conditions. It was observed in these studies that biosorption under acidic conditions is not favoured in several species as at low pH the protons (H+) compete with UO22+ for sorption sites (surface hydroxyl groups–SOH), thus indicating poor selectivity of the biosorbent surface against competing ions.
In order to understand the interaction between the cell and metallic species in wastewater, the cell surface can be thoroughly characterised using Fourier Transform Infrared spectroscopy (FTIR) or Energy-Dispersive X-ray spectroscopy (EDX). In our research group at the University of Pretoria, we were able to demonstrate that different functional groups on the cell wall of sulphate reducing bacteria acted as ion exchange sites in different pH ranges.
Using autotitration date in the software MINTEQ, four equilibrium states were identified which were associated with carboxylic functional groups (pKa 4-5), phosphates (pKa 6-7), phenolics (pKa 8-9), and hydroxyl/amines (pKa 10-12). The most abundant reaction sites in sulphate reducing bacteria (SRB) were associated with the hydroxyl functional groups. Adsorption of the divalent fission product (Sr2+) was inhibited at higher pH, supposedly as a result of increased hydroxylation at the high OH- concentration in solution. Additionally, increased pH could increase the formation of SrII-OH precipitates which is counterproductive to the processes of adsorption to the cells.
Biosorption offers a unique advantage in that the biosorbent media (bacteria) is self-regenerating and can be safely disposed after expiry. Apart from the uranium species, the biosorbent can remove a range of other toxic heavy metals from the wastewater without creating hazardous sludge at costs much lower than conventionally used ion exchange systems. Regeneration of the biosorbent and concentration of the metal solution for eventual recovery further increase the cost effectiveness of the process.
2.7. Bioaccumulation
Bioaccumulation is an active process wherein metals are taken up into living cells and sequestrated intracellularly by complexing with specific metal-binding components or by precipitation. Intracellular accumulation of metals occurs among all classes of microorganisms by an energy-dependent transport system. Unlike metabolically essential metals such as Fe, Cu, Zn, Co, and Mn, which accumulates intracellularly via energy transport system, intracellular uranium sequestration is attributed to non-specific transport system mainly due to increased membrane permeability resulting from uranium toxicity in the living cell [34]. Therefore, intracellular accumulation of uranium is considered as metabolism-independent process. The major drawbacks associated with the use of active uptake systems is the requirement of metabolically active cells and also the challenge in metal desorption and recovery [35]. Specifically, the cells will need to be destroyed to release the metal either by lysis or by incineration. Therefore, in this case, the medium for the uptake of metals cannot be reused.
2.8. Bioprecepitation
Bioprecipitation also known as biocrystallization or biomineralization is the process by which metals and radionuclides can be precipitated with microbially generated ligands such as phosphate, sulphide or oxalate [35]. In these processes bacteria interact strongly with metal ions and concentrate them, eventually generating carbonates and hydroxide minerals at the surface of the cell. Macaskie et al. [35] investigated
2.9. Bioreduction
Reduction of highly toxic and mobile U(VI) to sparingly soluble U(IV) using appropriate microbes has been proposed as a mechanism for preventing the migration of U(VI) with groundwater [37, 38]. The strategy is based on injecting physiological electron donors such as acetate, lactate, ethanol, or glucose to stimulate U(VI) reduction by microbial community native to contaminated aquifers [39]. Microorganisms are known to have evolved biochemical pathway for degradation or transformation of toxic compounds from their immediate environment either for survival or to derive energy by using toxic compounds as electron donors or acceptors [40, 41]. The overall transfer of electrons from the carbon sources to active uranium species can be visualised by the figure below (Figure 1).
An example of a balanced stoichiometric relationship during U(VI) reduction using propanoate as an electron donor is represented by Equation 1 (below):
Microbial reduction of U(VI) was first reported in crude extracts from
2.10. Enzymatic U(VI) reduction
Members of genera
2.10.1. Desulfovibrio reductase
The enzymatic system responsible for U(VI) reduction, tetraheme or periplasmic cytochrome-
2.10.2. Geobacter reductase
Genome sequence of
2.10.3. Shawanella reductase
Among the species that conserve metabolic energy from dissimilatory respiration utilising U(VI) an electron sink is
By assay of mutants, several proteins including the one involved in menaquinone biosynthesis, decaheme outer membrane cytochrome, a periplasmic decaheme cytochrome, outer membrane protein, and a tetraheme cytochrome were all shown to be needed for optimal U(VI) reduction [55]. The multiple pathways for electron delivery to U(VI) available in
2.11. Permeable reactive barriers
Waste from power generation and fuel process facilities contains high levels of uranium and transuranic elements. This type of waste, classified as high level waste (HLW) or transuranic waste (TU), is usually solidified in a concrete or bitumen before it is stored in specially engineered facilities above the ground. The chances of environmental contamination from such facilities are slight. However, most of the voluminous intermediate level and low level waste (ILW and LLW) can be packed and stored underground. The underground storage facilities pose a high risk of groundwater contamination. Where contamination has actually occurred, pump-and-treat processes are utilised to intercept the polluted groundwater for treatment above ground. The water can be treated using chemicals or using biological reactors and the clean effluent is returned to the aquifer. For toxic metals, chemical agents may be added followed by precipitation to reclaim the metals [62]. The chemical reduction process utilizes toxic reducing agents that produce toxic sludge requiring further treatment before disposal into natural waters. Biological processes have been proposed for the pump-and-treat process, but this does not eliminate the problem of disposal of the product of the precipitation stage. Several techniques for installing a biological barrier have been attempted such as construction of semi-porous walls which require a fair amount of excavation (Figure 2), injection of nutrients to encourage the growth of certain types or native species in the environment (a form of bioaugmentation), and inoculation of a region down gradient of a pollutant with specialized cultures of bacteria. Molecular
The decreasing concentration of U(VI) across the barrier is envisioned if barrier is inoculated with U(VI) reducing bacterial species. In the case of U(VI) reduction across a barrier system, we hope to utilise U(VI) as an electron sink in a dissimilatory respiration process in which the organisms introduced in the barrier (
The main limitation of
3. Determination of U(VI)
U(VI) reductase activity was determined by measuring the decrease in U(VI) in the solution using UV/vis spectrophotometer (WPA, Light Wave II, and Labotech, South Africa). Arsenazo III (Sigma-Aldrich, St. Louis, Missouri, USA) (1, 8-dihydroxynaphthalene-3, 6 disulphonic acid-2, 7-bis [(azo-2)-phenylarsonic acid]), a non-specific chromogenic reagent, was selected as the complexing agent for facilitating U(VI) detection. The accuracy and the precision of the method on the UV/vis spectrophotometer was determined by measuring the concentration of standard U(VI) solution in the range of (0-80 mg/L). A linearized U(VI) standard curve was generated by plotting the absorbance at 651 nm versus the known U(VI) standard concentration. Standard curve for U(VI) measurement demonstrated high degree of accuracy with R2 = 99.7% and was used to estimate unknown U(VI) concentration.
Measurement of U(VI) was carried out by withdrawing 2 mL of homogenous solution from a 100 mL serum bottle using a disposable syringe. The sample was then centrifuged for about 10 minutes at 6000 rpm (2820
4. Uranium (VI) reduction capability in pure cultures
In order to achieve
Microorganisms were isolated from the soil samples collected from tailings dumps of an abandoned uranium mine. Background uranium concentration in the original sample was detected at levels as high as 29 mg/kg, much higher than values observed in natural soils (0.3-11.7 mg/kg). To select U(VI) tolerant species, microorganisms from soil were cultured overnight into a 100 mL of sterile basal mineral medium (BMM) amended with glucose as sole carbon source and a dose of U(VI) (75 mg/L uranium (VI) as uranyl nitrate). The inoculum was grown under anaerobic conditions for 24 hours at 30±2°C in 100 mL serum bottles purged with pure (nitrogen) N2 gas (99.9% pure grade) for about 5-10 minutes to expel residual oxygen before sealing the bottle with rubber stoppers and aluminium seal. After 24 hours enriched bacterial strains were isolated by serial dilution of the cultivated culture. U(VI) reduction activity was evaluated for the isolates starting with evaluation for abiotic processes to make sure that physical-chemical processes are taken into consideration when analysing the biological process.
5. Abiotic U(VI) reduction
Heat-killed cultures and sodium azide exposed cultures were used to determine the extent of abiotic U(VI) reduction in batch experiments. For U(VI) reduction experiments cultures were grown over night in a sterile nutrient or Luria-Bettani (LB) broth under anaerobic conditions. Overnight grown cells were heat killed by autoclaving at 121°C for 20 minutes and another set of overnight grown cells were incubated with 0.1% of sodium azide (NaN3). Cells were then harvested by centrifuging at 6000 rpm (2820
The results showed insignificant difference U(VI) reduction between live and heat-killed cells (Figure 3). The instantaneous U(VI) reduction in heat-killed cells may be due to the presence of the cells that escaped destruction by heat. The reduction of U(VI) observed during the first 2 hours in all treatment containing biomass presented an anomaly. It was clear from these results that another mechanism rather than the direct metabolic process was involved in the U(VI) removal from solution. On the other hand abiotic control (without bacteria) showed that U(VI) reduction process is biologically mediated.
Reverting back to the biosorption studies, it is suggested that functional groups on the cell wall surfaces (-OH, -NH2, and –COOH) may serve as ligands for U(VI)-U(IV) complexation with the cell surface. U(VI) reduction may serve as a step towards this complexation step. To evaluate these effects we conducted experiments where the pH was varied and the oxidation reduction potential (ORP) was measured with time. Results presented in Figure 4 show that that the rate of U(VI) reduction was pH dependent (Figure 4a). Electronegative conditions under anaerobic conditions created a strongly reducing environment as expected, after which the ORP increased to electropositive values (Figure 4b). As a result insignificant change over time in ORP indicated poor oxidation-reduction, while significant change in ORP over time indicated that the oxidation-reduction process approaches completeness.
6. U(VI) Reduction by non-purified cultures (Consortium)
Preliminary experiments were conducted over a wide range of U(VI) concentration (30-400 mg/L) under similar experimental conditions (100 mL serum bottles containing BMM amended with D-glucose, U(VI) solution, and then incubated at 30±2°C under anaerobic conditions) using a reconstituted consortium culture of several identified U(VI) tolerant species. Results showed complete U(VI) reduction in batch cultures at initial U(VI) concentration up to 300 mg/L within 24 hours. In all batch studies with U(VI) concentration up to 400 mg/L (80-100%) U(VI) removal was achieved within the first 5 hours of incubation. However, inhibition of the reduction process was observed at the initial U(VI) concentration of 400 mg/L over time (Figure 5a).
U(VI) reduction trends in batches using purified cultures with single species per batch showed similar trends of U(VI) reduction. Figure 5b shows the summary of results from the best performing cultures labeled Y1, Y5, and Y6. The species characterisation results for these pure cultures are presented later in the chapter. The results in Figure 5b show that the microorganisms existing as a community possess significant stability and metabolic capabilities which can be linked to the effectiveness of synergistic interactions among members of bacterial communities [64].
7. Fate of reduced uranium species in a cell
Proportional distribution of uranium precipitates in the medium and cells can be used to determine the location of U(VI) reductase activity in the culture system. This is because most the precipitates are formed from reduced uranium species. Transmission electron microscopy (TEM) was used to establish the distribution and localization of uranium deposits in the cells. The energy dispersive X-ray (EDX) spectrometer coupled to the TEM was also used for elemental characterization of the metal deposits in the medium. TEM result images show crystal structures in the medium and very little crystallisation inside the cells (Figure 6a). EDX analysis of the crystals deposited on the cell surfaces confirmed the accumulation of uranium elements in the crystal matrix. Extracellular depositions of uranium also indicate that bacteria are excellent nucleation sites for mineral formations. EDX spectra derived from the uranium deposits show that they are composed of the following elements U>Cu>P>Os>Ca>Co>Fe (according to their descending order of their weight %). The higher copper (Cu) peak results from the specimen to support grid used. Phosphorous observed in the spectrum could either be from the added phosphorous or could microbially produced. On the other hand no uranium was observed in the metal free biomass (Results not shown).
8. Microbial characterisation and activity
8.1. Correlation of U(VI) reduction to enzyme activity
Proteins make up a large fraction of the biomass of actively grown microbes. To determine microbial activity over time, protein concentration was determined using a UV/Vis Spectrophotometer (WPA, Light Wave II, and Labotech, South Africa) at the wavelength of 595 nm using Coomassie Dye as a complexing agent to facilitate protein detection. Samples required pre-treatment to reduce interferences during the spectrophotometric analyses. Cell lysis was achieved by ultrasonification of acid treated cells. Results showed that microbial activity decreased with increasing U(VI) reduction (Figure 7). These results served as a confirmation of enzymatic activity as responsible agent for U(VI) reduction.
8.2. Culture composition analysis
The phylogenetic characterization of cells from the mine dump soil was conducted after sub-culturing the cells in nutrient or Luria-Bettani broth. Individual colonies from a serially diluted preparation were carefully examined for colony morphology and cell morphology by Gram-staining. This process, we recognize, could eliminate a wide range of potential U(VI) reducers especially anaerobic species in the samples. But at this stage, we were targeting the species that can survive under facultative anaerobic conditions.
The purified colonies were streaked on nutrient agar followed by incubating at 30°C for 18 hours in preparation for 16S rRNA gene sequence analysis. Microbial pure cultures were grown from loop-fulls from individual colonies, transferred to fresh media containing low amounts (30-75 mg/L) of uranyl nitrate. The process was repeated at least three times for each colony type to achieve close to a pure culture of each identified species.
Genomic DNA was extracted from purified colonies according to the protocol described for the Wizard Genomic DNA purification kit (Promega Corporation, Madison, WI, USA). 16S rRNA genes were amplified by a reverse transcriptase-polymerase chain reaction (RT-PCR) using primers pA and pH1 (Primer pA corresponds to position 8-27; Primer pH to position 1541-1522 of the 16S gene under the following reaction conditions: 1 min at 94°C, 30 cycles of 30s at 94°C, 1 min at 50°C and 2 min at 72°C, and a final extension step of 10 min at 72°C). PCR fragments were then cloned into pGEM-T-easy (Promega) [Promega Wizard® Genomic DNA Purification Kit (Version 12/2010)]. The 16S rRNA gene sequences of the strains were aligned with reference sequences from
U(VI) reducing colonies were identified from the genera
In the phylogenetic analysis, the scale indicated at the bottom of the plots, e.g., 0.005 for Figure 8a represents the genetic distance, while the percentage numbers at the nodes indicate the level of bootstrap based on neighbour-joining analysis of 1000 replicates. The three species related to
9. U(VI) removal kinetics
9.1. Kinetic model adaptation
To model a biological U(VI) reducing system, the reaction scheme, rate equations and kinetic constants for the processes taking place in the batch reactor are chosen from published models on enzymatic reduction hexavalent toxic metals such as U(VI). Shen and Wang [67] demonstrated that the rate of U(VI) reduction by enzymes can be expressed as the Monod equation if viable cell concentration
where:
where:
U(VI) reduction data obtained with the pure cultures and the mixed culture were analyzed using Equation 4. Parameters in Equation 4 can be analyzed using simulation software such as AQUASIM or SigmaPlot. The model is calibrated using batch data over the incubation period. The values collected under non-inhibiting conditions are suitable for estimating the kinetic parameters
9.2. Uranium (VI) reduction under inhibiting conditions
The inhibition model is suitable for application where the U(VI) loading per cell is very high. This is expected during startup (inoculation) of a systems with U(VI) already present. Such would be the case during the initial operation
where:
9.3. Continuous flow systems
Continuous flow systems better simulate actual systems especially where
where:
Where:
The reaction term
where:
10. Conclusion
The chapter addresses the main feature of various U(VI) remediation techniques involving the
Nomenclature
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