AE test parameters
\r\n\tNot all mixtures of particles and liquids can be considered slurries. A slurry has its character quite different from the carrying liquid (sometimes referred to as the vehicle). A Newtonian liquid has its shear stress directly proportional to its rate of deformation, but this is seldom the case for a slurry. In general, slurries are referred to as non-Newtonian liquids and ways of dealing with them are important threads in this text.
\r\n\r\n\tPipe blockages and pipe wear cause high costs to industry, in both maintenance and loss of production. This waste, and environmental damage which comes with it, can be shown to be reduced by careful application of slurry technology. This book will welcome recent research efforts to understand slurries related to the above-mentioned topics.
",isbn:"978-1-80356-669-6",printIsbn:"978-1-80356-668-9",pdfIsbn:"978-1-80356-670-2",doi:null,price:0,priceEur:0,priceUsd:0,slug:null,numberOfPages:0,isOpenForSubmission:!0,isSalesforceBook:!1,isNomenclature:!1,hash:"a3de73ad02868797334aa3024ec3f018",bookSignature:"Dr. Trevor Jones",publishedDate:null,coverURL:"https://cdn.intechopen.com/books/images_new/11907.jpg",keywords:"Slurry Rheology, Non-Newtonian Flows, Wastewater Treatment, Blood Rheology, Slurry Measurement, Slurry Tomography, Pipeline Pigs, Pipeline Cleaning, Wear, Swirl Induction, Electrical Resistance Tomography, Electrical Capacitance Tomography",numberOfDownloads:null,numberOfWosCitations:0,numberOfCrossrefCitations:null,numberOfDimensionsCitations:null,numberOfTotalCitations:null,isAvailableForWebshopOrdering:!0,dateEndFirstStepPublish:"March 22nd 2022",dateEndSecondStepPublish:"May 25th 2022",dateEndThirdStepPublish:"July 24th 2022",dateEndFourthStepPublish:"October 12th 2022",dateEndFifthStepPublish:"December 11th 2022",dateConfirmationOfParticipation:null,remainingDaysToSecondStep:"6 hours",secondStepPassed:!1,areRegistrationsClosed:!1,currentStepOfPublishingProcess:2,editedByType:null,kuFlag:!1,biosketch:"Dr. Jones is a world-leading expert in naturally-occurring particle products - slurries, sludges, coal, ore, and gravel. A professional engineer with many years of research experience in the minerals industry and academia at the University of Nottingham, UK, Dr. Jones now operates his own engineering consultancy.",coeditorOneBiosketch:null,coeditorTwoBiosketch:null,coeditorThreeBiosketch:null,coeditorFourBiosketch:null,coeditorFiveBiosketch:null,editors:[{id:"248406",title:"Dr.",name:"Trevor",middleName:null,surname:"Jones",slug:"trevor-jones",fullName:"Trevor Jones",profilePictureURL:"https://mts.intechopen.com/storage/users/248406/images/system/248406.jpg",biography:"Dr Trevor Jones is an engineering professional with extensive experience of industry and research over many years. Following 17 years as a project leader and ultimately head of Department of Beneficiation at the research centre of British Coal, Dr Jones was a researcher at the University of Nottingham, UK where he was supervisor for 5 doctoral students and lecturer in Mechanics for the undergraduate programme. In 2004 he started his consultancy business TFJ Consulting Ltd and was responsible for high value commissions for the nuclear industry. 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AE sensors are used to detect the acoustic waves at the surface of a structure, produced by AE events either on the surface or in the bulk of the material [2].
There are two types of signals in the AE system; namely burst and continuous signals. Burst signal is a separate type of signal of a very short duration (in the range of a few microseconds (µs) to a few miliseconds (ms)) and it is a broad frequency domain spectrum. Meanwhile the continuous signal is emitted close to each other or the burst is very high rate. The continuous signal is also occurred very close and sometimes overlaps. When the AE signal or output is transmitted in a structure, an array is identified. The output is always represented in a waveform which has information on a source location. A key to compute a source location is by determination of the wave velocity of the wave propagation. If incorrect wave velocity was used either owing to poor assumption or triggering of the system, it would affect to the determination of the source location [3]. Thus accurate wave velocity is important for the determination of the source location prior to any AE test.
The AE wave velocity can be determined by estimating the time of arrival (TOA) of the wave propagating in the structure; normally based on the threshold level [3] or frequency [4]. Typically, the propagation of wave in concrete can be categorized into three different types; namely dilatation wave (compression waves, longitudinal wave or P-wave for primary), the distortion wave (shear waves or S-wave for secondary) and the Rayleigh wave (surface wave or R-wave) [5–7]. Longitudinal wave is known as L – wave or P – wave; which the wave travels in the material and Rayleigh wave (R-wave) travels along the surface of the specimen. The initiation time of the P-wave is when the first TOA of the elastic wave reaches to each sensor. If the onset of the S-wave is detected, this information can be used either in combination with P-wave or be considered as P-wave onset [8]. Determining S–wave is challenging because the distance between sensor and receiver is only few wavelengths. Another problem is because the onset of the S–wave is hidden in the P–wave [8]. Generally, an S-wave is normal to the direction of the wave propagation and a P-wave, parallel. Determination of onset time can be done visually or automatically. This depends on the onset definition itself and also by picking algorithm. In relation with the wave velocity, the P-wave travels at a higher velocity than S-wave [9]. In concrete, the S-wave velocity is approximately 40% less than of the P-wave velocity and R-wave velocity is 92 % of the S-wave velocity and 56 % of the P-wave velocity [10]. However, only the P-wave would be considered in this study based on the TOA.
In order to determine the wave velocity, AE signals are produced by pencil lead fracture (PLF), known as Hsu-Nielsen technique. In this technique, PLF is the monopoles which is normally applied to the outside of the test sample, and the real AE signal is nearly dipoles in which the AE sources that originated from the points are buried inside the sample [11]. The previous PLF was used to generate simulated acoustic emission signals in an aluminum plate at different angles; 0, 30, 60 and 90 degree with respect to the plane of the plate [12]. Next, it is suggested that the plate wave analysis be used to determine the source orientation of acoustic emission sources. A few researchers have used the same method in the heterogeneous material as well as reinforced concrete (RC) [3, 13]. To verify the wave velocity propagation in the RC beam, corrected signal was used by Muhamad Bunnori [3], by normalizing each signal for peak value of amplitude up to 10 volts. The same method was also utilized for this analysis.
In this study, various threshold levels were performed to determine the TOA and the wave velocity in the RC beams with shorter source-to-sensor distance. Very little attention has been given on the shorter distance between sensor and sensor to source distance. An AE source was specified and focused on out-of plane and in-plane of the arrangement of sensor faces. Three main objectives are addressed. Firstly to study the relationship between wave velocity and sensor distance, secondly to investigate the relationship between wave velocity and threshold level and lastly to observe the relationship between arrival time and sensor distance.
The test of wave velocity was carried out on a reinforced concrete (RC) beam with dimension of 150 mm x 150 mm x 750 mm; designed in accordance with British Standard [14] for grade C40. The concrete was made up from cement, water, fine aggregate and coarse aggregate with proportion of 1: 0.43: 2.16: 2.60, respectively. Then, 1 % of water reducer agent (Rheobuild 1100) of cement weight was added in the concrete mix to improve the workability of the fresh concrete. The maximum coarse aggregate of 20 mm was used.
The beams were designed as a singly RC beam with two high yield steels of 16 mm to strengthen the tension part and two mild steels of 8 mm as hanger bars. The bars were bent at both end to form a standard hook of 60 mm at tension part and 30 mm at compression part. In the stirrups, 12 diameter of mild steel with spacing of 100 mm centre to centre was used. In the preparation of each RC beam, the reinforcement was submerged in the standard mould of 150 mm x 150 mm x 750 mm with cover of 20 mm, before the concrete mix was cast. All beams were demoulded after ±24 hours and submerged in the water curing tank for 28 days. In order to ensure the design fulfilled the strength, the cubes were prepared and tested under compression test for 7 and 28 days. The compressive strength for 7 days was found to be 32.35 N/mm2 and 44.65 N/mm2at age of 28 days.
Acoustic emission (AE) was monitored using a MicroSAMOS (µSAMOS) supplied by Physical Acoustic Corporation (PAC). The system consists of integral preamplifier acoustic emission sensors (transducers) R6I (40 – 100 kHz); a notebook acoustic emission system board (8 (channels) x 16 (hubs) bit acoustic emission channels, low peak and high peak filters, 2 MHz bandwidth, auto sensor test (AST), time definition display (TDD), digital signal processor (DSP) and waveform module; personal computer memory card international association (PCMCIA) interface card; cables; internal and external parametric cable set; a notebook personal computer with full suite of AEWin Software; universal serial bus (USB) license key; and magnetic clamps. The acquisition parameters in the AEWin software were summarized in Table 1.
Parameter | Value |
Hit definition time (HDT) | 2000µs |
Peak definition time (PDT) | 1000µs |
Hit lockout time (HLT) | 500µs |
Preamplifier (R6I) | 40dB |
Bandpass data acquisition filter | 400kHz |
Sample rate | 100ksps |
Analog filter (lower) | 1kHz |
Pre-trigger | 250,000 |
AE test parameters
Prior to the wave velocity test, three pairs of thin plates were prepared to attach the sensors S1, S2 and S3 with the distance of 200 mm and 100 mm between pair of plates. Spacing between two plates is 45 mm. The beam surface was polished smoothly using course sand paper to ensure good connectivity between sensors and beam surface. The plates were then fixed to the beam surface using epoxy and hardener.
Three sensors were coupled on the beam surfaces at the selected point using a good couplant such as a thin layer of high performance grease. The thinnest practical layer of couplant is usually the best [15]. The magnetic clamps were used to safeguard the sensors; which sensors held in position in magnetic clamps; then the magnetic clamps properly coupled to the steel plates.
With sensors in good mounting condition, calibration (sensitivity) checking was carried out to recognize the sensitivity of the sensors. In this experiment, a magnetic pencil with a Nielson shoe (Teflon shoe) was used to break a 0.5 mm 2 H lead to generate a simulated acoustic wave against the surface of the beam. The handling method of the magnetic pencil has been presented in ASTM E976 [16]. All sensors would significantly coupled if the wave was generated by at least three or more replicates of pencil lead fracture (PLF). This might produce high amplitude of 99 dB or the sensitivity within ± 3 dB in different [3, 17]. Based on the research done by others, it was found that the amplitude recorded by each sensor should not be permitted to vary more than 4 dB from the average of all sensors [18]. This technique was used to ensure that the sensor and the beam were in a good contact to provide an adequate result throughout the test. If these criteria are not met, the sensors on the beam surface would be remounted and sensitivity check was carried out until the amplitude fulfilled the requirement.
In this experimental work, three integral preamplifier R6I sensors with 55 kHz resonant frequency were used. Acoustic emission sensors are transducers that convert the mechanical waves into electrical signal [19], where the information about the existence and location of possible damage or stress released sources can be obtained. The specification and feature of the sensor is shown in Table 2.
Specification | Value |
Dimension (Ø x height) mm | 29 x 40 |
Weight (gm) | 98 |
Operating temperature (0oc) | -45 to +85 |
Shock limit (g) | 500 |
Peak sensitivity (V/m/s) | 120 |
Directionality (dB) | +1.5 |
Specification and feature of the R6I sensor
A schematic diagram of the test set up for beam size of 150 mm x 150 mm x 750 mm and 100 mm x 100 mm x 500 mm in a linear structure is shown in Figure 1 and Figure 2, respectively. The AE sources were identified on the out-of plane and in plane of the sensors arrangement or source parallel to the sensor face. Three sensors were mounted in a symmetrical arrangement on the RC beam using a thin layer of high vacuum grease as a couplant with the same spacing of 200 mm centre to centre for beam 150 mm x 150 mm x 750 mm and 100 mm centre to centre for beam 100 mm x 100 mm x 500 mm.
In this experiment, four different pre-set threshold levels were chosen namely 40 dB, 45 dB, 65 dB and 70 dB. At each threshold level, 15 replicates of PLF were applied to generate a simulated AE source at the same spot or the same AE source location. When the lead is pressed against the RC beam surface, the applied force produces a local deformation and the stress where the lead touched is suddenly being released. It is important to handle the pencil properly while breaking the lead against the testing specimen to get significant value. Improper handling of the pencil would give imprecise value; which will affect the TOA. The proper breaking of the lead creates a very short-duration, localized impulse that is quite similar to a natural acoustic emission source such as crack [19]. The lead was extended to 0.1 inches and the pencil was slanted down 300 to the plane of the beam surface, as suggested by other researchers [18, 20]. The out-of plane AE source was located at the centre of the cross section of the end beam. As for in-plane AE source, it was located at 100 mm from Sensor 1 as illustrated in Figures 1 and 2.
In the AE hardware, for in-plane source, Sensor 1 was set as individual and the rest were synchronized. Meanwhile, for out-of plane source, Sensor 3 was set as individual and the rest were synchronized. The same method applied for beam size of 100 mm x 100 mm x 500 mm.
In acoustic emission, waveform parameters normally used are frequency and amplitude [22]. Amplitude of AE signal parameter was used in identification of wave velocity. It is defined as the magnitude of the peak voltage of the largest excursion attained by the signal waveform from single emission event [1]. Amplitude is reported in decibels (dB) to measure signal size and typical AE signal is represented as a voltage versus time curve. Voltage is converted to dB using the following equation:
A = 20 log (V/Vref) (1)
Where:
Schematic diagram of sensors location on the RC beam 150 mm x 150 mm x 750 mm
Schematic diagram of sensors location on the RC beam 100 mm x 100 mm x 500 mm
In a relationship between threshold level and amplitude, preamplifier was considered; where equation (2) was used to calculate amplitude:
Threshold = Amplitude – Preamplifier (2)
The rudimentary determination for location calculation is based on time-distance relationship implied by the velocity of the sound wave [4, 23]; where the absolute arrival time,
d = v t (3)
Generally the distance between the two sensors depends on the geometry of the sample. Determination of TOA (the exact time the event originated) was made by recording many data hits for each AE event.
ΔT = (
At selected source locations (either out-of plane or in-plane), 10 replicates of PLF were applied from the lower threshold level of 40 dB to the higher 70 dB. Distance for beam 150 mm x 150 mm x 750 mm from sensors 1-2 and sensors 1-3 is 200 mm and 400 mm, respectively. Meanwhile, for beam 100 mm x 100 mm x 500 mm, the distance from sensors 1 to 2 is 100 mm and sensors 1 to 3 is 200 mm.
The relationship between wave velocity and threshold level, and TOA and sensor distance were correlated. Both correlations were then verified by corrected AE signal. Corrected AE signal was used for a particular threshold level, the same temporal position in each waveform which involves the normalizing of each signal up to a peak value of 10 volts. In this research, the corrected signal was used for the evaluation of TOA for each threshold level. The corrected signal can be identified by normalizing the peak signal of a hit to 10 volts followed by other signal in a hit. Then, the TOA for each threshold was then determined.
Sensitivity checking was used to ensure the sensor and the beam was in a good contact to provide an adequate results. The sensors must have a higher sensitivity [24] in order to get good contact between sensors surface and beam surfaces. In this experimental in order to check the sensitivity, at least ten numbers of 99 dB were considered for each sensor. The responses of the transducer to PLFs are shown in Figure 3. Sensor 1 represents a good sensitivity since almost of all the PLFs produced amplitude of 99 dB. However, Sensor 2 needs several trials to ensure the sensors and the beam surface have a good contact. These scenarios that occurred in Sensors 1 and 2 are related with the signal waveform produced from the PLF. It is typically affected by the source characteristics, the path taken from the source to the sensors, the characteristic of the sensor and the measurement system [25]. The waveform for Hit 1 and Hit 12 are represented in Figure 4.
Response to PLF on reinforced concrete beam for a) Sensor 1 and b) Sensor 2
AE waveform for two different AE amplitudes during sensitivity checking
Generally, each PLF produces one signal of AE hit or more and in various form or shape of waves. Figure 4 shows different shapes of wave for two hits of PLF from Sensor 1. Hit 1 is a response of PLF that produces amplitude 99 dB and generates high peak wave. Hit 12 is for amplitude 91 dB response from the bounce back of the lead during PLF process and produced lower amplitude. Hit 1 generates high peak wave rather than Hit 12. Figure 3a indicated that Hit 12 occurred at the same time of PLF with other amplitudes. However, for sensitivity checking this will be ignored. The peak signal amplitude can be related to the intensity of the source in the material [21, 25]. Pullin et al. [26] stated that the good sensitivity between sensor and beam surface is enough when the amplitude signal exceeded 95 dB. Hence, sensitivity checking would be better when a higher amplitude is being produced during replicates of PLFs.
Typical AE waveform parameter received by Sensor 1 type R6I from a 150 x 150 x 750 mm RC beam during PLF process, represented in amplitude (v) against time (µs) is shown in Figure 5. As lead breaks (in-plane or out-of-plane) on the surface of the beam, a wave propagates through a solid medium; it carries certain amount of energy. The energy can be consumed by scattering during propagation. The scattering effect principally relies on the defects such as micro-cracks inside the material. In wave velocity determination only several AE parameters would be considered such as time and amplitude. AE amplitude gives the information about the time at which AE signals take place [27]. Wave velocity is one of the methods to be considered for source location. Figure 6 presents the wave of the signal for threshold 40 dB. The first wave crosses the threshold level is known as P-wave.
Simple waveform parameter
In this experiment, Sensor 1 was set as individual due to close to the AE source and the rest were considered as synchronize. For instance, the threshold was set at 40 dB prior to test. The Sensor 1 would be the first received the waves emitted by PLF at AE source followed by other sensors as depicted in Figure 6. In this figure, the TOA or longitudinal wave (P-wave) for Sensors 1, 2 and 3 were noted as
TOA or P-wave for threshold 40 dB at time 35.53 s for each sensor
The first method in the determination of wave velocity in 150 mm x 150 mm x 750 mm and 100 mm x 100 mm x 500 mm RC beam is based on the changes of pre-set threshold level in AE hardware or threshold crossing technique. Four different pre-set threshold levels were chosen namely 40 dB (0.01V), 45 dB (0.02V), 65 dB (0.2V) and 70 dB (0.32V). For each pre-set threshold level, 10 replicates of PLF were carried out in-plane and out-of-plane sources. TOA to each sensor for each PLF was analysed and followed by other replicates of PLFs. Figure 7a shows the relationship between the average wave velocity and sensor distance for out-of-plane source at each threshold level. Figure 7b presents the relationship of average wave velocity and sensor distance for in-plane source. Both figures show the wave velocities are apparently depends on the sensor distance and threshold levels.
In determination of wave velocity, sensors 1, 2 and 3 was set of 0 m, 0.2 m and 0.4 m, respectively. Figure 7 illustrates that, for RC beams 150 mm x 150 mm x 750 mm with sensor distances 0.2 m, at lower threshold level (40 dB) and higher threshold level (70 dB) for out-of-plane source, the velocity is in the range of 7889 m/s to 4559 m/s and 2409 m/s to 1654 m/s, respectively. Meanwhile in in-plane source, velocities for lower threshold and higher threshold are in the range of 3086 m/s to 2608 m/s and 2619 m/s to 2192 m/s, respectively. It is found that the wave velocity for in-plane source has lower velocity than out-of-plane source. It might be due to the in-plane source location is parallel to the sensor surface and the wave propagates take longer time to reach the sensors. Hence, the source location in linear measurement of sensors for in-plane would prolong the TOA of the wave to reach the sensor and the velocity is reduced. Out-of-plane source is longitudinal to the sensor surface and hence the wave signal propagates inside the RC beam take shorter time to reach the sensors. Thus, it reduces the TOA of the wave to each sensor.
In wave velocity calculation for beam 100 mm x 100 mm x 500 mm, sensors 1, 2 and 3 was set as 0, 0.1 m and 0.2 m, respectively. Figure 8 presents the wave velocity in 100 mm x 100 mm x 500 mm with sensor distances of 0.1 m for out-of-plane. The figure shows that the lower threshold level is in the range of 3430 m/s to 2810 m/s and the higher threshold level is 2765 m/s to 1166 m/s. Velocity propagates in in-plane source is in the range of 5030 m/s to 3150 m/s for threshold 40 dB and 2751 m/s to 2603 m/s for threshold 70 dB. In this case, apparently the velocity in the in-plane source has higher velocity than out-of-plane source. Logically, the pattern of the wave velocity occurred in the both size of beams with different spacing of sensors should be synchronized. However, this phenomenon indicates that the wave velocity in the RC beam or heterogeneous material cannot be predicted.
Wave velocity in 150 mm x 150mm x 750 mm RC beam for a) out-of-plane source and b) in-plane source
The results of the study were also compared to other research done in heterogeneous material as well as RC structure of the wave velocity of RC beam 100 mm x 150 mm x 2000 mm with the sensor distance of 250 mm, the value was in the range of 4000 m/s to 4500 m/s [3]. For large spectrum with frequency between 50 to 600 kHz, the wave velocity is 2350 m/s [28]. The wave velocity in ceramic matrix composite was 3200 m/s with the threshold level of 48 dB; however, the initial velocity was 10000 m/s [29]. If resonant sensor of 300 kHz was used, the wave velocity is 4000 m/s [30]. For carbon fibre reinforced polymer (CFRP) the wave velocity equal to 1000 m/s [13].
Wave velocity in 100 mm x 100 mm x 500 mm RC beam for a) out-of-plane source and b) in-plane source
Figures 9a and 9b display the velocity propagates in the 150 mm x 150 mm x 750 mm RC beam as threshold levels increased for out-of-plane and in-plane sources. As mentioned earlier, apparently the wave velocities depend on the threshold levels. Thus, the statistical coefficient can be used to enhance the prediction by identification of determination coefficient, R2. For out-of-plane source, for sensors distance 0.2 m (Sensor 3-2), the R2 is 91.3 % and for distance 0.4 m (Sensor 3-1) produced higher R2 of 97.8 %. Thus, both distances have strong correlation between velocities and also the threshold level, where, the wave velocity in the RC beam is influenced by the threshold level crossing. The higher the threshold level, the lower of the wave velocity would be.
For in-plane source, distance between Sensors 1 to 2 and 1 to 3 is 0.2 m and 0.4 m, respectively. It is found that for in-plane source, both distances of sensors have weak correlation of 30.3 % for Sensors 1-2 and 49 % for Sensors 1-3. It is due to the wave velocities for 40 dB are lower than for threshold 45 dB with the approximate different in the range of 2100 m/s to 940 m/s. However, after threshold of 45 dB, the wave velocity seems has good correlation, which the threshold increased as the wave velocity decreased.
Figure 10 shows the relationship between wave velocity propagation and threshold level for 100 mm x 100 mm x 500 mm RC beam. Wave velocity for closet distance in Figure 10 shows a good relationship between velocity and threshold levels with the correlation of 83 % and 92 % for out-of-plane and in-plane source, respectively. However the longer distance represents a weak relationship with the correlation of 59 % and 40 % for out-of-plane and in-plane source, respectively. Overall the prediction of the wave velocity in short distance is influenced by threshold levels has strong correlation. However, for longer distance, the relationship is seemingly unpredictable.
Relationship between velocity and threshold level on RC beam 150 mm x 150 mm x 750 mm for a) out-of-plane source and b) in-plane source
Relationship between velocity and threshold level on RC beam 100 mm x 100 mm x 500 mm for a) out-of-plane source and b) in-plane source
It can be concluded that the relationship of the velocity and threshold level is easy to predict for shorter distance, where the velocity decreases when the threshold level increases. It apparently indicates a good correlation for shorter distance. However, for longer distance the relationship is unpredictable and has weak relationship. This can be related with the material applied, where concrete always has imperfection of the composition such as voids.
Figure 11 shows the average of arrival time (µs) against sensor distance for original signal and corrected signal for out-of-plane source at threshold 40 dB, 45 dB, 65 dB and 70 dB on 150 mm x 150 mm x 750 mm RC beam. In the graphs, Cor stands for corrected signal and Ori stands for original signal. The corrected signal has been used to justify the correlation between TOA and distance. Sensor 3 (distance zero) close to the AE source, it receives the first TOA emits by PLF and follows by the other sensors. Sensor 1 would receive the last signal with the higher TOA. In some situation, the farthest sensors from the AE source would not receive any signal. This phenomenon occurs because wave attenuation in the RC beam cannot be avoided. Wave attenuation is loss of AE energy as waves travel through in a material [25]. It is also the loss of amplitude with distance as the wave travel through the beam [7]. This is also affected of the time taken path of the signal wave from the AE source to the sensors. The farthest sensor would receive the lower signal wave and hence take longer TOA. For instance, Original 40 dB at fist sensor is 29 µs and the farthest sensor is 40 µs. Thus, TOA takes longer time to reach sensor 1. Other factor causes the waveform amplitude loss such as intrinsic mechanisms and imperfections. It is because the intrinsic mechanism (thermal effects) and imperfections (void and misoriented grains) reduce the amplitude of the wave by using scattering and reflections [4]. Otherwise, RC beam is a heterogeneous material; many obstacles such as aggregates and bars affected to the movement of waves and yet delayed the TOA to the longest distance from the AE source. If the attenuation is too large or size of the structure is too big, the location of the hit cannot be defined. Thus, the attenuation depends on the types of material through which the waves are progressing and the source of the waves [7].
Regression analysis of arrival time against sensor distance for 150 mm x 150 mm x 750 mm RC beam (out-of-plane source) at a) 40 dB, b) 45 dB, c) 65 dB and d) 70 dB
All figures show very good correlation between TOA and sensor distance for out-of-plane source and there is insignificant difference between original signal and corrected signal. All threshold levels have strong correlation with the determination of coefficient more than 97 % between TOA and sensor distance.
Figures 12 a), b), c) and d) represent the regression analysis of arrival time against sensor distance for in-plane source at threshold 40 dB, 45 dB, 65 dB and 70 dB, respectively. Similar to the one represented in Figure 11, it shows that all threshold levels have good correlation between time of arrival (TOA) and sensor distance with all the coefficient of determinations more than 96 %. However, threshold level of 40 dB for corrected signal analysis apparently has a perfect fit of linear correlation by representing the coefficient equal to 1. The value indicates that the TOA is absolutely influenced by the sensor distance. Similar to out-of plane source, the relationship also indicates no significant difference between original signal and corrected signal.
Figure 13 represents the regression analysis of TOA against sensor distance for 100 mm x 100 mm x 500 mm in-plane source at all threshold levels. Similarly, it shows that it has good correlation with the regression above 80 % with no significant difference between original signal and corrected signal.
Regression analysis of arrival time against sensor distance for 150 mm x 150 mm x 750 mm RC beam (in-plane source) at a) 40 dB, b) 45 dB, c) 65 dB and d) 70 dB
Overall, the TOA of the wave propagation has good agreement with distance of sensors, where the longer the distance, the higher the time taken to reach the sensor. It can be seen in Figures 8 and 9, the slope of the best straight line drawn through the points are linear. However, other researchers found the relationship between TOA and sensor distance is not linear [3]. Since, the TOA is a good indication of wave velocity travels in the beam, hence the wave velocity decreases as the TOA increases.
Regression analysis of arrival time against sensor distance for 100 mm x 100 mm x 500 mm (in-plane source) at a) 40 dB, b) 45 dB, c) 65 dB and d) 70 dB
Three sensors were used to determine the TOA and the wave velocity in RC beams. Sensor 1 was set as individual since it is close to the AE source and the first to receive the waves (P-Wave) that were emitted by PLF in the solid medium of concrete.
It can be concluded that AE wave velocities were found dependent on threshold levels and distance of sensor. It is found that the higher the threshold levels, the lower the wave velocity. This is because the threshold level prolonging arrival times of the waves and reducing velocities. Wave velocities for 150 mm x 150 mm x 750 mm were calculated to be in the range of 7889 m/s to 1654 m/s for out-of-plane source and 5229 m/s to 2192 m/s for in-plane source. Wave velocities for 100 mm x 100 mm x 500 mm were computed to be in the range of 3430 m/s to 1166 m/s for out-of-plane source and 5030 m/s to 2603 m/s for in-plane source. Wave velocities decreases with the increasing distance between sensors and threshold levels. It was enhanced by the relationship of wave velocities and sensor distance for each threshold level.
The relationship between wave velocity and threshold level were identified for each sensor distance and then the coefficient of correlation, R2 was determined. It is found that R2 for beam 100 mm x 100 mm x 500 mm at out-of-plane source has good correlation than in-plane source. Beam 150 mm x 150 mm x 750 mm has weak correlation for longer sensor distance, but it has good correlation for shorter distance.
The relationship of TOA and sensor distance was identified. Its correlation was presented for each original signal and corrected signal. In total, both original signal and corrected signal indicated strong correlation between TOA and sensor distance with domain regression of 90 %.
The authors gratefully acknowledge the support provided by the Universiti Teknologi MARA (for financial support), Universiti Sains Malaysia (for the AE equipment) and Ministry of Higher Education, Malaysia (MOHE). A special thank you to all technicians in the Laboratory of Heavy Structure, Faculty of Civil Engineering, Universiti Teknologi MARA and Universiti Sains Malaysia.
The increase in human activities triggers environmental pollution through the generation and disposal of hazardous wastes in aquatic and terrestrial habitats [1]. Most of these pollutants include inorganic (heavy metals) and organic matter (polyaromatic hydrocarbons (PAH), petroleum hydrocarbons compounds (PHC)) which may cause negative effects on the ecosystem and possibly react with other abiotic factors that attribute to the effect on the structural arrangement of terrestrial and aquatic habitats [2].
In terms of the environment and ecology system, the proper and safe disposal of these hazardous wastes is a key priority for a sustainable ecosystem. This involves the use of various treatment procedures to clean up hazardous waste. For detoxifying heavy metals, radionuclide and organic polluted soils, physicochemical techniques such as filtration, precipitation, electrochemical treatment, soil washing and chelating, oxidation/reduction, ion exchange, reverse osmosis, and stabilization/solidification have been employed. These environmental clean-up procedures have various disadvantages, including inefficiency, the need for a large number of chemical reagents, energy, and high cost, as well as the formation of secondary by-products [3].
Bioremediation is a cost-effective and environmentally tolerable technology that employs a biological process to reduce environmental risks caused by toxic substances and other hazardous pollutants. To treat polluted multiphase systems and sustain the native ecosystem, a combination of bioremediation techniques will be effective. The fundamental premise of bioremediation is to reduce contaminant solubility by adjusting pH, modifying redox processes, and adsorbing toxic substances from polluted sites [3]. Environmental remediation always requires human assistance to achieve effective remediation of contaminants and restoration of ecological balance. However, remediation can be destructive to the ecosystem [4], if the application is not properly addressed to meet the eco-friendly standard required to combat the contemporary issues of pollution [4, 5]. Most small-scale applications of bioremediation approaches using bioremediation agents such as bacteria, fungi, plants, and organic materials have been successful with variation in results, although bioremediation on a large scale has not been widely validated [4]. This chapter aims to propose a cost-effective and eco-friendly bioremediation strategies that could reduce or remove contaminants from the environment and thus stabilizing the ecosystem from heavy metal pollution and oil spills.
There are a lot of different physical, chemical, and biological processes commonly termed bioattenuation, which make pollutants smaller in terms of their size and toxicity as well as how much of them there are. Some examples of these processes are sorption, volatilization, chemical or biological stabilization, and the transformation of contaminants. This entails removing pollutant concentrations from the surrounding through biological methods or perhaps incorporating (oxic and anoxic biodegradation, plant and animal sorption), physical occurrences (changes in weather conditions, dispersion, dilution, diffusion, volatilization, sorption/desorption), and chemical reactions (ion exchange, complexation, abiotic change) [6, 7, 8]. For instance, natural biodegradation and biotransformation are incorporated within the broader notion of common restriction [9, 10]. At the point when the site is contaminated with chemicals, the environment acts in 4 different approaches to facilitate remediation [11]:
Microbes or microorganisms living in soil and groundwater may consume just a small number of chemical or manmade chemicals available as dietary nutrients. When they have completely digested the chemical, they can convert it to water and non-toxic gases.
Chemical compounds can stick to or sorb to the soil, which prevents them from contaminating groundwater or escaping the location.
As contamination travels through soil and groundwater, it can blend in with clean water. This diminishes or weakens the contamination.
Certain chemicals, such as oil and solvents, can disappear, hence, they can transform from liquids to gases within the soil or groundwater. As a result, if these gases reach the earth surface via the air, they may be pulverized by sunlight.
Additionally, if natural attenuation is insufficiently rapid or complete, bioremediation will be accelerated or augmented via biostimulation, bioaugmentation, bioventing, or biopile [11, 12].
This bioremediation approach invigorates the activity of native microbes by adjusting the environmental parameters or the introduction of nutrients [11, 13]. This is carried out with the incentive of natural or normally prevailing parasites or microbial communities [7, 11, 13]. Successive steps involve providing manures, development enhancements and minor elements. Also, by giving other natural prerequisites including pH, temperature and oxygen to enhance their digestion rate and degradation pathway [10, 12]. Similarly, the presence of pollutants even in small quantities can act as a stimulant by spinning for bioremediation proteins. Typically, this type of deterioration is followed by the provision of organic or inorganic nutrients and oxygen to promote the metabolism of native microbes for effective remediation [6]. These nutrients are the fundamental building blocks of life, enabling microorganisms to synthesize vital components such as enzymes, energy, and cell biomass required to degrade the toxin [6, 14]. However, nitrogen, phosphorous and carbon are significantly required to enhance metabolism.
This procedure entails sequentially adding contaminant-degrading microbes (inherent/non-native/genetically modified) to improve the biodegradative efficiency of the native microbial community in the polluted site [8, 11]. Thus, to rapidly grow the natural microbial population and accelerate breakdown at the pollutant’s location. Microorganisms that predominate in polluted sites on a global scale, may surely change significant amounts of harmful substances into non-poisonous structures. This process converts pollutants to by-products like carbon (IV) oxide and water, as well as metabolic intermediates that serve as critical nutrients for cell development [15, 16]. Microorganisms can also be isolated from the remediation environment, cultured autonomously, genetically engineered, and then reintroduced to the site [8, 11]. For persuade, all basic microbes are prevalent in locales where soil and groundwater are polluted with chlorinated ethenes, for example, tetrachloroethylene and trichloroethylene [7, 8, 11]. These are employed to facilitate the effective removal and conversion of these pollutants to non-poisonous ethylene and chloride by in situ microbes [10].
Additionally, genetically modified microbes have been shown to degrade a broad range of environmental contaminants effectively. Since the metabolic pathway can be altered to produce less puzzling and harmless end products [8, 17]. Genetically engineered microorganisms (GEM) have shown viability in bioremediation of soil, groundwater and activated sludge, proving effective degradation abilities of extensive integration of chemical and physical contaminations. GEMs have better enzyme abilities, which makes them better at breaking down a wide spectrum of aromatic hydrocarbons and making the soil more fertile [14, 18]. There are several types of hydrocarbon-degrading microorganisms that include the genera
It is the practice of venting oxygen through the soil to encourage the development of natural or injected microbes and fungus in the soil by supplying oxygen to the soil microbes, which has been termed as bioventing [8, 11, 14]. The use of low air flowrates to supply sufficient oxygen to sustain microbial movement has long been a typical practice in aerobic degradation of substances, and it has been for many years. For example, several scientists have demonstrated that bioremediation of oil-contaminated soil utilizing bioventing may be achieved with reasonable success [19]. Consequently, petroleum residuals and their by-products are biodegraded, and volatile organic compounds, when destroyed, release vapors that slowly permeate through the biologically dynamic soil environment.
Biopile, also known as biocells, bioheaps, biomounds and composts piles are employed to minimize the toxicity of total petroleum hydrocarbon constituents via microbial respiration. Biopiles are an ex-situ bioremediation technology that consists of piling polluted soil onto a compost pile (biopiles) or cells (biocells) or mounds (biomounds) or heaps (bioheap) and stimulating oxic metabolism in the soil via aeration or introduction of minerals or nutrients, bulking agents, and subsequently confining it in a treatment bed with polyethylene material to avoid evaporation, surface runoff, and volatile emissions. Biopiles treatments can transform pollutants into low-toxic by-products through biological processes by utilizing already existing microorganisms to breakdown fuels and oils into carbon dioxide and water.
The biopile technology is made up of commercial roll-off dumpsters or containers that have been turned into fully contained bioremediation units. The biopile units have an impermeable liner to decrease the possibility of leachate movement to the subsurface ecosystem. Excavated soils are combined with soil additives and placed on a treatment area with leachate collecting devices and some type of aeration to maximize and regulate the rate of biodegradation. Air is introduced to the biopile mechanism of piping and pumps, which either power air into the heap under a specific tension or draw air through the heap under a negative tension [8, 20]. Microbial movement, for instance, can boost the adsorption and degradability of petroleum pollutants during funneling and siphoning operations. Biopiles, such as biocells, bioheaps, biomounds, and compost, might alleviate public concern about excavated soil contaminated by vigorously remediable hydrocarbons [8, 13, 19].
Utilizing plants for bioremediation is highly dependent on their ability to break down certain pollutants [21, 22, 23, 24]. Phytoremediation is the process of utilizing plants to degrade, eliminate, or convert contaminants to less hazardous chemicals [25]. Even though plants have been used for soil purification for centuries, scientists have contributed to its advancement and expanded its scope of application throughout the years [7, 11, 13, 14, 17, 18]. This involves the removal of metals, pesticides, solvents, explosives, and raw petroleum, as well as a variety of other pollutants from soils, water (surface and subsurface), and vaporous contaminants [7, 11, 14]. When the plants have accumulated enough toxins, they are harvested and disposed of. Figure 1 shows a graphical presentation of different types of phytoremediation as each mechanism is explained as follows:
Phytostabilization: this entails using plants to minimize soil erosion, so immobilizing contaminants by limiting their movement and accessibility in the soil via the plant roots. Additionally, it prevents metals from moving to the soil or the surface of underground water.
Phytovolatilization: this involves the use of plants to minimize soil erosion, so immobilizing contaminants by limiting their movement and accessibility in the soil via the plant roots. Additionally, it prevents metals from moving to the soil or the surface of underground water.
Phytodegradation: this process includes the degradation or modification of pollutants in the plant tissue by enzymes.
Phytoextraction: this approach involved the extraction of contaminants from the soil and their accumulation in the shoots. Upon that, these plants’ leaves are gathered, burned for energy, and the metals retrieved from the ash are regenerated.
Phytofiltration or rhizofiltration use roots to accumulate and sequester contaminants from polluted water.
Phytostimulation or rhizodegradation: plant roots are employed to digest organic pollutants in the rhizosphere environment and through microbial activity.
Schematic presentation of phytoremediation process (adapted from [
This is when two or more bioremediation methods work together to remove contaminants from the environment. This kind of bioremediation technique can be effectively applied in a multi-contaminated environment. The combinative strategy most likely to be suitable and effective in boosting bioremediation of bauxite residue is a combination of bioaugmentation (incorporation of inocula) [8, 11] and biostimulation (introduction of nutrients to enhance the activity of microorganisms) of the indigenous community in bauxite residue [11, 13].
In this scenario, for instance, biostimulation using organic or inorganic compounds can be applied as the first or basic treatment while bioventing or bioaugmentation using engineered microbes can be applied subsequently as a secondary or tertiary treatment to facilitate the removal or degradation of recalcitrant compounds. Combinations of bioaugmentation and biostimulation have also proven effective, albeit they do not always show significant improvements over bioaugmentation alone. Given the nearly consistent advancement seen with bioaugmentation technology, it is anticipated that bioaugmentation will improve on the outcomes obtained so far with biostimulation for bauxite waste cleanup (provided an appropriate choice of the microbes and adequate trials are prioritized). Based on the simplicity of obtaining and introducing the inoculum, the most suited approach for future research and field trials is combinative bioremediation using biostimulation and bioaugmentation technology.
Several bioremediation mechanisms for reducing or oxidizing contaminants have been discovered over time, such as adsorption, physio-biochemical (biosorption and bioaccumulation) bioleaching, biotransformation, biomineralization, and molecular mechanisms [7, 11].
Environmental pollutants (both organic and inorganic) can be absorbed by microorganisms at specific sites in their cell structure that do not require the dissipation of energy. There are many various kinds of chemicals connected with bacterial cell walls, but their extracellular polymeric substances (EPS) are of particular importance since they have been shown to have significant effects on corrosive base characteristics and metal adsorption [10, 26]. Several studies on the metal binding behavior of EPS have revealed that it has a remarkable capacity to absorb complex metals by a variety of processes that combine ion exchange and micro-precipitation of metals [10, 13]. Bioremediation research and application are still limited in the present scenario due to a lack of understanding of the genetic traits and genome-level properties of the organisms used in metal adsorption, the metabolic route, and the kinetics of metal adsorption [7].
In microscopic organisms, inhibition is advanced through two mechanisms: detoxifying (changing the detrimental metal’s state and rendering it inaccessible) and dynamic efflux (siphoning poisonous heavy metals from cells) [7, 9]. In wastewater or soil, the fundamental redox (oxidation and reduction) reaction occurs between hazardous metals and microorganisms. Additionally, microbes oxidize heavy metals, causing them to lose electrons, which are recognized by active electron acceptors (nitrate, sulphate and ferric oxides) [26]. Additionally, the biosorption process, which consists of a biosorbent’s increased affinity for sorbate (metal ions), is repeated till a balance between the two components is established [18, 26]. For instance,
Bioaccumulation is a term referring to the combination of active and passive techniques of hazardous metal bioremediation. Additionally, bioremediation may entail aerobic or anaerobic microbial activity [10, 12, 13]. Aerobic degradation frequently involves the addition of oxygen atoms to the reactions via monooxygenases, dioxygenases, hydroxylases, oxidative dehalogenases, or chemically active oxygen molecules produced via catalysts including ligninases or peroxidases [10, 11, 12, 13]. Anaerobic contaminant corruptions comprise initial enactment reactions followed by oxidative degradation with the assistance of anaerobic electron acceptors. The act of Immobilization refers to the process of reducing the activation of significant metals in a polluted environment by modifying their physical or synthetic state [7, 12]. Microbes muster metals from polluted sites through leaching, filtering, chelation, methylation and redox transformation of harmful metals [12, 17]. Since significant metals cannot be entirely eliminated, the cycle modifies their oxidation state or organic complex to make them more soluble, less poisonous and precipitated [9, 14].
In bioleaching, naturally occurring microorganisms such as bacteria and fungi solubilize metal sulphides and oxides from ores and secondary wastes. Adsorption, ion exchange, membrane separation, and selective precipitation are some of the processes used to purify solubilized metals. It is a cost-effective and environmentally beneficial technique because it consumes less energy and produces no hazardous gases. It has been applied to leach metals from low-grade ores, and it now provides a substantial global business in the extraction of metals like copper, cobalt, gold, nickel, uranium, zinc, and other elements [27].
This is the procedure for altering the structure of a chemical substance to produce a molecule with higher polarity. Moreover, this metal-microbe interaction process converts hazardous metal and organic chemicals into a less poisonous form. This mechanism has emerged in microorganisms to assist them in adjusting to variations in their surroundings. Bacterial cells have a significant surface-volume ratio, a rapid pace of proliferation, a rapid rate of metabolic activities, and are easy to keep sterile [27]. As a result, they are perfect for biotransformation. Various methods, such as condensing and hydrolyses, forming new carbon bonds, isomerization, inserting functional groups, and oxidation, reduction, and methylation, can be used to attain this objective. Metals may be volatized, reducing their lethal nature, as a result of these interactions.
Biomineralization refers to the mechanisms by which microbes produce minerals, and it can lead to metal extraction from solution, which can be used for decontamination and biorecovery. Dead biota and related products may also serve as a model for mineral deposition, with physicochemical parameters determining whether the process is reversible or not. There are several prevalent microbe-precipitated biominerals with unique chemical features such as high metal sorption capacities and redox catalysis. However, some biominerals can be deposited at nanoscale dimensions, resulting in additional physical, chemical, and biological features that can be used in practical applications [28].
Different components of genetically altered bacteria, such as Deinococcus geothemalis, are active in the removal of heavy metals [9, 14, 18]. Hg2+ reduction has been recorded at high temperatures as a result of the expression of meroperon from
The promising bioremediation technique involves the application of bioturbators. Bioturbation is made up of a series of processes triggered by microbenthic fauna that influences sediment physicochemical characteristics and affects the microbial population which partake in the distribution of nutrients [29]. Bioturbation involves a series of activities such as the reworking of particles, bioirrigation, and other benthic biota related behaviors (i.e. nutrition mode and grazing by animals and organisms) that were responsible for transportation and distribution of porewater and particles along the water-sediments interface [30]. The distribution of dissolved contaminants can be a reworking of sediments by bioturbators through facilitating transportation and biomixing efficiency from overlying water and porewater to deep layers of the sediment [31, 32, 33].
The term “bioturbation” relates to the procedure of completely transforming dangerous hazardous substances into harmless or naturally occurring chemicals. Bioturbation can be done in situ (for example, in field conditions) or ex-situ (for instance, in a microcosm or under controlled conditions). Both scenarios entail the utilization of plants, parasites/fungi, and microorganisms as bioremediators for the biodegradation of toxic pollutants, even though individualized end product may be a different component [34, 35, 36]. Thus, complete breakdown of the contaminants by the bioremediators directly or indirectly may influence the residue structure [34, 37]. Figure 2 presents significant types of contaminant improvement approaches by bioturbators (benthic fauna) in the contaminated environment to facilitate residue treatment.
Schematic representation of bioturbators activities in sediments (i) biodiffusors, (ii) upward conveyors, (iii) downward conveyors, and (iv) regenerators.
Biodiffusors: this is performed through microorganisms’ activities, which often result in the biomixing of uniform and irregular sediments over short separations, resulting in particle interchangeability via molecular diffusion.
Upward conveyors: these are organisms that live vertically head-down in the sediments. They transfer particles from the residue’s deep horizons to its surface. Gravity then returns the particles to the base under the influence of feces pellet agglomeration at the sediment surface.
Downward conveyors: these are head-up feeders that actively pick and consume particles near the surface, as well as discharge in deeper residual layers.
Regenerators: these microorganisms dive into the leftovers and constantly maintain burrows, so transferring dirt from depth to the surface.
The role and effectiveness of bioturbators in bioremediation is dependent on several conditions, such as the chemical type and quantities of contaminants, the physicochemical properties of the environment, and their accessibility to microbes [38]. Bioturbators are responsible for vital changes in the biological and physicochemical aspects of soils and water [38, 39]. Additionally, aerobic bioturbation can increase benthic digestion and supplement components by stimulating oxygen-consuming bacterial networks that are concerned with pollutant mitigation [8, 11]. In other words, bioturbators are well-suited for a dual-purpose mechanism, namely the production of degradative enzymes for specific contaminants and resistance or protection from significant relative dangerous substances such as heavy metals [15, 38, 39]. Controlling and simplifying bioremediation procedures is a difficult process due to a large number of components including the presence of a microbial community with the ability to detoxify pollutants, the contaminants’ accessibility to the microbial community, and abiotic conditions (soil type, temperature, pH, oxygen or other electron acceptors, and substrates) [6, 16, 39].
Bioturbation influences the sediment-water interface’s biological, physical, and chemical properties which accounts for the high rate of mineralization of organic matter in the aquatic environment [40]. This operation changes the sediment column distribution of the contaminants [41]. Bioturbation and biotransport can affect the physicochemical characteristics of sediments and sediment pollutants [42, 43, 44]. Bioturbation controls the organic matter and nutrient digestion enhances pollutant mobility and transformation [45, 46, 47, 48]. The biosorption of organic contaminants into the organic matter during bioremediation reduces its bioavailability for plants (phytoremediation) or degrading organisms (bioaugmentation) [49]. Atrazine removal from sediments is promoted and positively influenced by the adjustment of organic matter and earthworm bioturbation activities, which increases contaminant bioavailability and atrazine sorption rate on their microsites [46, 50]. Previous studies reported positive contributions of earthworm bioturbation to organic pollutant transformation and biodegradation [51, 52] by modifying pore size and metabolism of degrading bacteria groups or accelerating mineralization in bioaugmented soils [50].
Moreover, several studies showed that bioturbation alters the physicochemical characteristics of the water-sediment boundary which promotes the bioavailability of inorganic pollutants to degrading organisms. This is achieved through the modification of sediment particle sizes, pore spaces, moisture content, nutrient content, turbidity, and total organic carbon of the vadose water-sediment [41, 43, 53]. Also, the bioturbation of benthic invertebrates through the mixing of sediments in the underground zone enhanced the electron acceptors (oxygen, nitrate and sulphate) entrance into the vadose zone which triggers geochemical changes that influence metal behavior [54]. The presence of these electron acceptors in the unsaturated zone can activate the RedOx reaction to change the chelating of metals affinities between liquid and solid phases to enhance the quantitative distribution and bioavailability of metal in the sediment [55]. The changes created by the bioturbation-attributed redox potentials, pH, organic content, pore spaces can affect metal sorption capacity and improve metal conversion from one phase to another e.g. Cd, Zn [56, 57, 58].
The activities of bioturbators are affected by some factors which modulate the rate of bioturbation for effective remediation of polluted environments. These factors include the variation in salinity, temperature, density, sediment grain size pH, and concentration.
Variation in salinities in the aquatic environment can influence the metabolism of nutrient and metal releases [59, 60], whether naturally and/or through human-related activities. Remaili et al. [61] noted that hypersalinity has a negative effect on the larger bioturbators which affects the activities of benthic organisms. Gonzalez et al. [62] study found that the salinity levels and tolerance of various bioturbators are distinct. The findings however suggest that ammonia release in the aquatic environment is significantly modified due to the effect of modulating conditions and distinguished by a higher salinity than other nutrients such as phosphorus [62, 63].
Regional variability in temperature is also a crucial factor that regulates the impact of bioturbation in pollutant remediation. In microbial response, metabolism, and degradation of organic matter and metals, temperatures played a fair modulatory function [64]. In the presence of bioturbation activities, the rise in temperatures increases the production of ammonium from the sediment, possibly due to the high level of hydrogenase in microbial species and the increased aerobic conditions in the sediment [64, 65]. Gonzalez et al. [62] reported that an increase in temperature is indirectly proportional to the nutrient dispersion as high temperature decreases nutrient flux (phosphorus) in the sediment but extreme temperatures may be devastating to the microbes. However, an increase in temperature corresponds to the increased rate of metal resuspension and metal solubility as a result of higher bioturbation rates [66, 67].
The bioturbator density influences bioturbation, control bioturbation efficiency for contaminant remediation, which correlates with the increased aerobic microbial activity and emission of pollutants. The increased bioturbation density increased phosphorus release and induced aerobic microbial activity but did not increase the release of ammonia. Animal density is a highly imperative factor, as study reveals that higher densities contribute toward greater degradation and mineralization of organic matter but may also increase nutrients in the overlying water and can, depending on the ecosystem studied, have counterproductive effects on recovery [66]. In response to pollution, the population of certain benthic species such as polychaetes [68] may increase as several systems are deprived of the use of other larger bioturbators.
Another element that influences the high level of organic matter and metals accumulation and the structure and metabolism of microbial communities and their metabolism is the sediment grain size [69, 70]. A recent study also shows a positive association between ammonia, phosphorus release, and aerobic microbial activity for the sediment grain size as Martinez-Garcia et al. [70] noted that the grain size showed less effect at low organic enrichments, but instead, at higher enrichments, coarse sediments contain less organic matter and nutrients while metabolism rate is enhanced. The contaminant bioavailability assessment can be affected by the susceptibility, grain size and behavior of microbes used in bioassays or observed on the ground, and the interaction between various species and microbial populations in highly polluted sediments depauperated by larger invertebrates [1, 71].
The concentration of organic or inorganic contaminants is another factor that regulates the activities of the benthic organisms [72] which tend to either reduce or hinder the activities of the benthic organism at a high concentration, beyond the tolerable limit, which can result in the death of these organisms at extreme condition due to toxicity [5]. Benthic organisms have varying tolerance limits for sediment contaminations and tend to possess special features or activities (such as bioaccumulation or biosorption) to enable them to adapt and function effectively in high pollutant concentrations. For metal remediation, abiotic factor-like pH which works closely with concentration may be a crucial modulating variable that determines the impact of bioturbation in the marine environment which can alter metal speciation and reactivity [66].
Therefore, sediment properties like particle size and concentration as well as contaminant shape (sulphides or organic carbon) can affect the bioavailability of the contaminant. Also, in most environments, temperature and type of organism activity or population density can increase or decrease contaminant exposure or bioavailability for bioremediation [61, 73, 74, 75].
Notwithstanding the benefits (such as environmental friendliness, selectivity, adaptability, self-reproducibility, and the ability to recycle bioproducts) of the bioremediation technique, some setbacks have hindered the successful application of this technology. The delay of the operations and the complexity in managing the procedures are the two most significant disadvantages of this technique of treatment. Since the elimination of significant concentrations of heavy metals is a priority, and that the world has become more aware of the environmental concerns caused by other approaches, microbial procedures offer the most rational and long-term answer for treatment. As previously stated, while a variety of microbial contaminant bioremediation techniques to address contamination have been developed, their extensive use and application on a commercial scale are still restricted by some factors. A further point to mention is that the long-term viability of microbial decontamination is still a subject of significant importance, given the paucity of investigations into its long-term performance. Due to the extremely high accumulation of inorganic contaminants (heavy metals) in heavily inhabited places of the world, updating existing microbial bioremediation technologies to an industrial level by making the procedures quicker, more reusable, and easier to regulate will be a big issue in the future. Furthermore, another limitation of bioremediation is that not all substances are biodegradable while some hydrocarbon components are recalcitrant to microbial breakdown, which restricts the scope of the remediation technique. Even when a material is biodegradable, its downstream operation and breakdown can result in the production of harmful by-products in some situations.
The potential for microorganisms to remediate water and soil pollutants to increase treated water consumption and soil fertility for agricultural output is gaining attention [11, 38]. Recently, research has been conducted to enhance the application of altered organisms delineated specifically to boost their affectability toward hazardous metals [11, 16, 38]. An organism whose genetics have been transformed by the use of synthetic methods, which are driven by an artificial genetic exchange between bacteria, is referred to as a “genetically engineered microorganism [11, 18]. By developing GEM, genetic engineering has enhanced the application and disposal of hazardous wastes in laboratory settings. In addition, the following protocols must be considered during the GEM process: (a) alteration of enzyme selectivity and affinity, (b) pathway development and modulation, (c) bioprocess advancement, surveillance, and control, and (d) bioaffinity bioreporter sensor utilization for chemical detecting, toxicity reduction, and endpoint evaluation [13, 18].
As there are several possibilities for improving degradation performance through genetic engineering approaches, such as genetically controlling the rate kinetics of known metabolic pathways to increase degradation rate, or completely infusing bacterial strains with new metabolic pathways for the degradation of previously recalcitrant compounds [6, 8]. Despite important genes for microorganisms are carried on a single chromosome, defining the specific genes needed for the catabolism of some of these novel substrates may be carried on plasmids [18, 76, 77]. Plasmids were entangled in the catabolism process. As a result, GEM can be successfully used for biodegradation purposes, necessitating immediate research and large-scale deployment. Genetically engineer microbes offer the benefit of developing microbial strains which can tolerate unfriendly upsetting circumstances and can be utilized as a bioremediation tool under different and complicated natural conditions [18, 37, 76, 77]. Additionally, GEM has encouraged the development of “microbial biosensors” capable of precisely quantifying the degree of pollution in a contaminated site.
The current advancement in omics technologies, including genomics, proteomics, transcriptomics, and metabolomics, play a critical role in finding characteristics that optimize remediation solutions [7, 11, 78]. Consequently, phytoremediation was developed, a process for eliminating toxins or their metabolites from plant tissues. This usually shortens the life of the plant and finally volatilizes the toxins into the atmosphere [78]. This disadvantage can be mitigated by managing plants’ metal resistance, accumulation, and breakdown capacity in the presence of various inorganic toxins. To improve metal decomposition in plants, bacterial genes responsible for metal reduction can be integrated into plant tissues. As a result, plant-based bioremediation for a variety of significant metal poisons is cutting-edge due to its eco-friendliness. They are more effective at reducing dangerous substances than Physicochemical approaches, which are less environmentally friendly and potentially detrimental to human health [7, 8].
Notwithstanding, microbial genes can bridle in the transgenic plant for decontamination and collection of inorganic pollutants [7, 11]. The metal-detoxifying chelators, for example, metallothioneins and phytochelatins can give resistance to the plant by upgrading take-up, transport and amassing of different heavy metals [14, 78]. Similarly, transgenic plants with bacterial reductase can augment the volatilization of Hg and Se while absorbing the arsenic in plant shoots [17, 78]. Also, high-biomass-producing plants including poplar, willow and Jatropha can be applied for both phytoremediation and energy generation [7, 14, 26, 78]. Nonetheless, metals can only be removed from soil or water, which is why consuming metal-contaminated plants is advantageous. Thus, metal-accumulating biomasses should be properly preserved or disposed of to avoid posing an environmental hazard [20, 78].
Bioremediation methods include the introduction of growth stimulators (electron acceptors/donors) or nutrients to the rhizosphere to promote microbial growth and bioremediation characteristics of microbes or genetically engineered plants [6, 26, 78]. Multiple small organisms were generated with heavy metals by drainage using synthesized catalysts such as chromate and uranyl reductase in a particular rhizosphere [19, 26, 78]. Although genomics has been studied and applied mostly in microbial genetics and agriculture, such as genetic crops, and now serve as a bioremediation instrument [26, 76]. The application of genomics to bioremediation enables the microorganism to be dissected based on biochemical constraints as well as sub-atomic levels associated with the component [26, 76, 77].
Bioturbation is a very prolific and appealing technology for remediation, cleaning, management, and recovery of environmental contamination caused by microbial activity [11]. Furthermore, phytoremediation is successful at removing both inorganic and organic pollutants from residues or soils [7, 11, 12]. Nonetheless, investigation of resourceful bioremediation approaches for damaged aquatic environments that are based on these two processes to improve wastewater and soil treatment is necessary [10, 17]. Nonetheless, investigation of resourceful bioremediation technologies based on these two processes is important to improve soil and wastewater treatment [11, 17]. In addition, phytoremediation has been generally illustrated as a bioremediation process for heavy metals, such as lead, cadmium, copper, arsenic removal from contaminated soil or water [76, 77]. In essence, aquatic bioturbation combined with phytoremediation is a more effective and alternative method of removing heavy metals by improving cadmium transfers from overlying water to sediment and then into the root system of plants [15, 38].
Additionally, studies have demonstrated that earthworm movement greatly boosted phytoavailability by increasing soil macroporosity and generating cast around plant roots (Figure 3), implying that the physical effect of the earthworm’s bioturbation is a viable mechanism [20, 26]. Interaction between plants and soil-dwelling microorganisms can also enhance phytoremediation known as rhizosphere bioremediation. The study by Leveque et al. [52] to investigate the contribution of earthworm (as bioremediator or bioturbation agent) to phytoremediation showed that earthworms significantly increased the phyto-availability of metal by generating soil macroporosity and developing cast near plant roots in which the main mechanism appears to be the physical impact of earthworm bioturbation. Moore et al. [21], demonstrated the contribution and the effect of bioturbators in the remediation of organic contaminants using the phytoremediation technique. In the study,
Proffered approach to illustrate metal phytoavailability in earthworms’ activities (adapted from [
The use of nanomaterials is extensively gaining attention for components remediation of heavy metals and recovery of valuable via nanotechnology [8, 34]. Conversely, nanobioremediation, which employs nanoparticles to stimulate microbial activity to clear hazardous chemicals from groundwater and soil [14, 17]. Not only can this nanotechnology greatly cut the cost of cleaning contaminated regions, but it also significantly shortens the procedure’s duration. Metal chelating polymers require damaging solvents for mixing and ultrafiltration for division, which can be avoided by inventing metal limiting substances that can be reclaimed by adjusting their pH, temperature, or form, among other parameters [13, 19, 20]. One of the materials is nanoscale modified biopolymers, produced by microorganisms’ intrinsic and protein structure, and whose size can be adjusted at the subatomic level [13]. For instance, polymers and magnetosomes are fabricated proteins for the remediation of infections,
The technique entails using ecological and environmental engineering expertise to create and monitor a sustainable ecosystem or biological system that benefits both humans and the environment. Table 1 and Figure 4 illustrate how to apply ecological engineering in a way that is more beneficial to humanity while maintaining the natural balance. Nevertheless, the majority of these technologies are typically designed with the following objectives in mind: (i) conservation, (ii) ecosystem restoration, (iii) expanding ecological systems to the quantity, quality, and maintainability of their production, and (iv) assembling new ecological systems that would provide routine types of assistance [16, 39, 76, 77, 80].
Ecological-engineering approaches | Terrestrial examples | Aquatic examples |
---|---|---|
Using ecosystems to solve a pollution problem | Phytoremediation | Wastewater wetland |
Imitating or copying ecosystems to reduce or solve a problem | Forest restoration | Replacement wetland |
Recovering an ecosystem after significant disturbance | Mine land restoration | Lake restoration |
Existing ecosystems are modified in an ecologically sound way | Selective timber harvest | Biomanipulation |
Using ecosystems for benefit without destroying the ecological balance | Sustainable agroecosystems | Multi-species aquaculture |
Application of ecological engineering approach for terrestrial and aquatic systems.
Graphical representation of ecological engineering application to balance the ecosystem.
Bioremediation is a cutting-edge and promising approach for treating contaminated soil and water. Microorganisms are also known to generate and use a variety of detoxification methods, including biosorption, bioaccumulation, biotransformation, and biomineralization for the remediation of the contaminated site during the bioremediation process. However, recent bioremediation research, such as bioturbation, which uses live organisms (macrofauna) directly or indirectly with the environment to eliminate toxins, is gaining momentum. The use of organisms to detoxify and recover polluted soil and water has emerged as the most robust, straightforward, and profitable technique. Microorganisms in water and soil have been studied and equipped to eliminate or detoxify harmful compounds discharged into the ecosystem due to anthropogenic processes such as mineral mining, oil and gas production, pesticides, pigments, plastic, organic solvents, fuel, and industrial operations. Nevertheless, a lack of data on microorganisms’ cell reactivity to minor components and heavy metal poisons precludes their successful implementation. As such, the application of molecular genetic technology will enhance the efficiency and address most of the challenges in the large scale application of bioremediation technology.
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He is a full professor of signal processing and pattern recognition and is head of the Signals and Communications Department at ULPGC, teaching from 2001 on subjects on signal processing and learning theory. His research lines are biometrics, biomedical signals and images, data mining, classification system, signal and image processing, machine learning, and environmental intelligence. He has researched in 52 international and Spanish research projects, some of them as head researcher. He is co-author of 4 books, co-editor of 27 proceedings books, guest editor for 8 JCR-ISI international journals, and up to 24 book chapters. He has over 450 papers published in international journals and conferences (81 of them indexed on JCR – ISI - Web of Science). He has published seven patents in the Spanish Patent and Trademark Office. He has been a supervisor on 8 Ph.D. theses (11 more are under supervision), and 130 master theses. He is the founder of The IEEE IWOBI conference series and the president of its Steering Committee, as well as the founder of both the InnoEducaTIC and APPIS conference series. He is an evaluator of project proposals for the European Union (H2020), Medical Research Council (MRC, UK), Spanish Government (ANECA, Spain), Research National Agency (ANR, France), DAAD (Germany), Argentinian Government, and the Colombian Institutions. He has been a reviewer in different indexed international journals (<70) and conferences (<250) since 2001. He has been a member of the IASTED Technical Committee on Image Processing from 2007 and a member of the IASTED Technical Committee on Artificial Intelligence and Expert Systems from 2011. \n\nHe has held the general chair position for the following: ACM-APPIS (2020, 2021), IEEE-IWOBI (2019, 2020 and 2020), A PPIS (2018, 2019), IEEE-IWOBI (2014, 2015, 2017, 2018), InnoEducaTIC (2014, 2017), IEEE-INES (2013), NoLISP (2011), JRBP (2012), and IEEE-ICCST (2005)\n\nHe is an associate editor of the Computational Intelligence and Neuroscience Journal (Hindawi – Q2 JCR-ISI). He was vice dean from 2004 to 2010 in the Higher Technical School of Telecommunication Engineers at ULPGC and the vice dean of Graduate and Postgraduate Studies from March 2013 to November 2017. 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He has (co)authored more than 150 publications in indexed journals, international conferences and book chapters, 1 book (in Greek), 3 edited books, and 5 journal special issues. His publications have more than 2100 citations with h-index 27 (GoogleScholar). His research interests include computer/machine vision, machine learning, pattern recognition, computational intelligence. \nDr. Papakostas served as a reviewer in numerous journals, as a program\ncommittee member in international conferences and he is a member of the IAENG, MIR Labs, EUCogIII, INSTICC and the Technical Chamber of Greece (TEE).",institutionString:null,institution:{name:"International Hellenic University",institutionURL:null,country:{name:"Greece"}}},editorTwo:null,editorThree:null},{id:"25",title:"Evolutionary Computation",coverUrl:"https://cdn.intechopen.com/series_topics/covers/25.jpg",isOpenForSubmission:!0,editor:{id:"136112",title:"Dr.",name:"Sebastian",middleName:null,surname:"Ventura Soto",slug:"sebastian-ventura-soto",fullName:"Sebastian Ventura Soto",profilePictureURL:"https://mts.intechopen.com/storage/users/136112/images/system/136112.png",biography:"Sebastian Ventura is a Spanish researcher, a full professor with the Department of Computer Science and Numerical Analysis, University of Córdoba. 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In the last five years, he has published more than 60 papers in international journals indexed in the JCR (around 70% of them belonging to first quartile journals) and he has edited some Springer books “Supervised Descriptive Pattern Mining” (2018), “Multiple Instance Learning - Foundations and Algorithms” (2016), and “Pattern Mining with Evolutionary Algorithms” (2016). He has also been involved in more than 20 research projects supported by the Spanish and Andalusian governments and the European Union. He currently belongs to the editorial board of PeerJ Computer Science, Information Fusion and Engineering Applications of Artificial Intelligence journals, being also associate editor of Applied Computational Intelligence and Soft Computing and IEEE Transactions on Cybernetics. Finally, he is editor-in-chief of Progress in Artificial Intelligence. 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conducts his research at the Hamidiye Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Division of Biochemistry, University of Health Sciences, Turkey. He is also a faculty member in the Molecular Oncology Program. He obtained his MSc and Ph.D. at Oregon State University and Texas Tech University, respectively. He pursued his postdoctoral studies at Rutgers University Medical School and the National Institutes of Health (NIH/NIDDK), USA. His research focuses on biochemistry, biophysics, genetics, molecular biology, and molecular medicine with specialization in the fields of drug design, protein structure-function, protein folding, prions, microRNA, pseudogenes, molecular cancer, epigenetics, metabolites, proteomics, genomics, protein expression, and characterization by spectroscopic and calorimetric methods.",institutionString:"University of Health Sciences",institution:null},{id:"180528",title:"Dr.",name:"Hiroyuki",middleName:null,surname:"Kagechika",slug:"hiroyuki-kagechika",fullName:"Hiroyuki Kagechika",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/180528/images/system/180528.jpg",biography:"Hiroyuki Kagechika received his bachelor’s degree and Ph.D. in Pharmaceutical Sciences from the University of Tokyo, Japan, where he served as an associate professor until 2004. He is currently a professor at the Institute of Biomaterials and Bioengineering (IBB), Tokyo Medical and Dental University (TMDU). From 2010 to 2012, he was the dean of the Graduate School of Biomedical Science. Since 2012, he has served as the vice dean of the Graduate School of Medical and Dental Sciences. He has been the director of the IBB since 2020. Dr. Kagechika’s major research interests are the medicinal chemistry of retinoids, vitamins D/K, and nuclear receptors. He has developed various compounds including a drug for acute promyelocytic leukemia.",institutionString:"Tokyo Medical and Dental University",institution:{name:"Tokyo Medical and Dental University",country:{name:"Japan"}}},{id:"40482",title:null,name:"Rizwan",middleName:null,surname:"Ahmad",slug:"rizwan-ahmad",fullName:"Rizwan Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/40482/images/system/40482.jpeg",biography:"Dr. Rizwan Ahmad is a University Professor and Coordinator, Quality and Development, College of Medicine, Imam Abdulrahman bin Faisal University, Saudi Arabia. Previously, he was Associate Professor of Human Function, Oman Medical College, Oman, and SBS University, Dehradun. Dr. Ahmad completed his education at Aligarh Muslim University, Aligarh. He has published several articles in peer-reviewed journals, chapters, and edited books. His area of specialization is free radical biochemistry and autoimmune diseases.",institutionString:"Imam Abdulrahman Bin Faisal University",institution:{name:"Imam Abdulrahman Bin Faisal University",country:{name:"Saudi Arabia"}}},{id:"41865",title:"Prof.",name:"Farid A.",middleName:null,surname:"Badria",slug:"farid-a.-badria",fullName:"Farid A. Badria",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/41865/images/system/41865.jpg",biography:"Farid A. Badria, Ph.D., is the recipient of several awards, including The World Academy of Sciences (TWAS) Prize for Public Understanding of Science; the World Intellectual Property Organization (WIPO) Gold Medal for best invention; Outstanding Arab Scholar, Kuwait; and the Khwarizmi International Award, Iran. He has 250 publications, 12 books, 20 patents, and several marketed pharmaceutical products to his credit. He continues to lead research projects on developing new therapies for liver, skin disorders, and cancer. Dr. Badria was listed among the world’s top 2% of scientists in medicinal and biomolecular chemistry in 2019 and 2020. He is a member of the Arab Development Fund, Kuwait; International Cell Research Organization–United Nations Educational, Scientific and Cultural Organization (ICRO–UNESCO), Chile; and UNESCO Biotechnology France",institutionString:"Mansoura University",institution:{name:"Mansoura University",country:{name:"Egypt"}}},{id:"329385",title:"Dr.",name:"Rajesh K.",middleName:"Kumar",surname:"Singh",slug:"rajesh-k.-singh",fullName:"Rajesh K. Singh",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329385/images/system/329385.png",biography:"Dr. Singh received a BPharm (2003) and MPharm (2005) from Panjab University, Chandigarh, India, and a Ph.D. (2013) from Punjab Technical University (PTU), Jalandhar, India. He has more than sixteen years of teaching experience and has supervised numerous postgraduate and Ph.D. students. He has to his credit more than seventy papers in SCI- and SCOPUS-indexed journals, fifty-five conference proceedings, four books, six Best Paper Awards, and five projects from different government agencies. He is currently an editorial board member of eight international journals and a reviewer for more than fifty scientific journals. He received Top Reviewer and Excellent Peer Reviewer Awards from Publons in 2016 and 2017, respectively. He is also on the panel of The International Reviewer for reviewing research proposals for grants from the Royal Society. He also serves as a Publons Academy mentor and Bentham brand ambassador.",institutionString:"Punjab Technical University",institution:{name:"Punjab Technical University",country:{name:"India"}}},{id:"142388",title:"Dr.",name:"Thiago",middleName:"Gomes",surname:"Gomes Heck",slug:"thiago-gomes-heck",fullName:"Thiago Gomes Heck",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/142388/images/7259_n.jpg",biography:null,institutionString:null,institution:{name:"Universidade Regional do Noroeste do Estado do Rio Grande do Sul",country:{name:"Brazil"}}},{id:"336273",title:"Assistant Prof.",name:"Janja",middleName:null,surname:"Zupan",slug:"janja-zupan",fullName:"Janja Zupan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/336273/images/14853_n.jpeg",biography:"Janja Zupan graduated in 2005 at the Department of Clinical Biochemistry (superviser prof. dr. Janja Marc) in the field of genetics of osteoporosis. Since November 2009 she is working as a Teaching Assistant at the Faculty of Pharmacy, Department of Clinical Biochemistry. In 2011 she completed part of her research and PhD work at Institute of Genetics and Molecular Medicine, University of Edinburgh. She finished her PhD entitled The influence of the proinflammatory cytokines on the RANK/RANKL/OPG in bone tissue of osteoporotic and osteoarthritic patients in 2012. From 2014-2016 she worked at the Institute of Biomedical Sciences, University of Aberdeen as a postdoctoral research fellow on UK Arthritis research project where she gained knowledge in mesenchymal stem cells and regenerative medicine. She returned back to University of Ljubljana, Faculty of Pharmacy in 2016. She is currently leading project entitled Mesenchymal stem cells-the keepers of tissue endogenous regenerative capacity facing up to aging of the musculoskeletal system funded by Slovenian Research Agency.",institutionString:null,institution:{name:"University of Ljubljana",country:{name:"Slovenia"}}},{id:"357453",title:"Dr.",name:"Radheshyam",middleName:null,surname:"Maurya",slug:"radheshyam-maurya",fullName:"Radheshyam Maurya",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/357453/images/16535_n.jpg",biography:null,institutionString:null,institution:{name:"University of Hyderabad",country:{name:"India"}}},{id:"311457",title:"Dr.",name:"Júlia",middleName:null,surname:"Scherer Santos",slug:"julia-scherer-santos",fullName:"Júlia Scherer Santos",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/311457/images/system/311457.jpg",biography:"Dr. Júlia Scherer Santos works in the areas of cosmetology, nanotechnology, pharmaceutical technology, beauty, and aesthetics. Dr. Santos also has experience as a professor of graduate courses. Graduated in Pharmacy, specialization in Cosmetology and Cosmeceuticals applied to aesthetics, specialization in Aesthetic and Cosmetic Health, and a doctorate in Pharmaceutical Nanotechnology. Teaching experience in Pharmacy and Aesthetics and Cosmetics courses. She works mainly on the following subjects: nanotechnology, cosmetology, pharmaceutical technology, aesthetics.",institutionString:"Universidade Federal de Juiz de Fora",institution:{name:"Universidade Federal de Juiz de Fora",country:{name:"Brazil"}}},{id:"219081",title:"Dr.",name:"Abdulsamed",middleName:null,surname:"Kükürt",slug:"abdulsamed-kukurt",fullName:"Abdulsamed Kükürt",position:null,profilePictureURL:"https://s3.us-east-1.amazonaws.com/intech-files/0030O00002bRNVJQA4/Profile_Picture_2022-03-07T13:23:04.png",biography:"Dr. Kükürt graduated from Uludağ University in Turkey. He started his academic career as a Research Assistant in the Department of Biochemistry at Kafkas University. In 2019, he completed his Ph.D. program in the Department of Biochemistry at the Institute of Health Sciences. He is currently working at the Department of Biochemistry, Kafkas University. He has 27 published research articles in academic journals, 11 book chapters, and 37 papers. He took part in 10 academic projects. He served as a reviewer for many articles. He still serves as a member of the review board in many academic journals.",institutionString:null,institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"178366",title:"Associate Prof.",name:"Volkan",middleName:null,surname:"Gelen",slug:"volkan-gelen",fullName:"Volkan Gelen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/178366/images/system/178366.jpg",biography:"Volkan Gelen is a Physiology specialist who received his veterinary degree from Kafkas University in 2011. Between 2011-2015, he worked as an assistant at Atatürk University, Faculty of Veterinary Medicine, Department of Physiology. In 2016, he joined Kafkas University, Faculty of Veterinary Medicine, Department of Physiology as an assistant professor. Dr. Gelen has been engaged in various academic activities at Kafkas University since 2016. There he completed 5 projects and has 3 ongoing projects. He has 60 articles published in scientific journals and 20 poster presentations in scientific congresses. His research interests include physiology, endocrine system, cancer, diabetes, cardiovascular system diseases, and isolated organ bath system studies.",institutionString:"Kafkas University",institution:{name:"Kafkas University",country:{name:"Turkey"}}},{id:"418963",title:"Dr.",name:"Augustine Ododo",middleName:"Augustine",surname:"Osagie",slug:"augustine-ododo-osagie",fullName:"Augustine Ododo Osagie",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/418963/images/16900_n.jpg",biography:"Born into the family of Osagie, a prince of the Benin Kingdom. I am currently an academic in the Department of Medical Biochemistry, University of Benin. Part of the duties are to teach undergraduate students and conduct academic research.",institutionString:null,institution:{name:"University of Benin",country:{name:"Nigeria"}}},{id:"192992",title:"Prof.",name:"Shagufta",middleName:null,surname:"Perveen",slug:"shagufta-perveen",fullName:"Shagufta Perveen",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/192992/images/system/192992.png",biography:"Prof. Shagufta Perveen is a Distinguish Professor in the Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. Dr. Perveen has acted as the principal investigator of major research projects funded by the research unit of King Saud University. She has more than ninety original research papers in peer-reviewed journals of international repute to her credit. She is a fellow member of the Royal Society of Chemistry UK and the American Chemical Society of the United States.",institutionString:"King Saud University",institution:{name:"King Saud University",country:{name:"Saudi Arabia"}}},{id:"49848",title:"Dr.",name:"Wen-Long",middleName:null,surname:"Hu",slug:"wen-long-hu",fullName:"Wen-Long Hu",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/49848/images/system/49848.jpg",biography:"Wen-Long Hu is Chief of the Division of Acupuncture, Department of Chinese Medicine at Kaohsiung Chang Gung Memorial Hospital, as well as an adjunct associate professor at Fooyin University and Kaohsiung Medical University. Wen-Long is President of Taiwan Traditional Chinese Medicine Medical Association. He has 28 years of experience in clinical practice in laser acupuncture therapy and 34 years in acupuncture. He is an invited speaker for lectures and workshops in laser acupuncture at many symposiums held by medical associations. He owns the patent for herbal preparation and producing, and for the supercritical fluid-treated needle. Dr. Hu has published three books, 12 book chapters, and more than 30 papers in reputed journals, besides serving as an editorial board member of repute.",institutionString:"Kaohsiung Chang Gung Memorial Hospital",institution:{name:"Kaohsiung Chang Gung Memorial Hospital",country:{name:"Taiwan"}}},{id:"298472",title:"Prof.",name:"Andrey V.",middleName:null,surname:"Grechko",slug:"andrey-v.-grechko",fullName:"Andrey V. Grechko",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/298472/images/system/298472.png",biography:"Andrey Vyacheslavovich Grechko, Ph.D., Professor, is a Corresponding Member of the Russian Academy of Sciences. He graduated from the Semashko Moscow Medical Institute (Semashko National Research Institute of Public Health) with a degree in Medicine (1998), the Clinical Department of Dermatovenerology (2000), and received a second higher education in Psychology (2009). Professor A.V. Grechko held the position of Сhief Physician of the Central Clinical Hospital in Moscow. He worked as a professor at the faculty and was engaged in scientific research at the Medical University. Starting in 2013, he has been the initiator of the creation of the Federal Scientific and Clinical Center for Intensive Care and Rehabilitology, Moscow, Russian Federation, where he also serves as Director since 2015. He has many years of experience in research and teaching in various fields of medicine, is an author/co-author of more than 200 scientific publications, 13 patents, 15 medical books/chapters, including Chapter in Book «Metabolomics», IntechOpen, 2020 «Metabolomic Discovery of Microbiota Dysfunction as the Cause of Pathology».",institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"199461",title:"Prof.",name:"Natalia V.",middleName:null,surname:"Beloborodova",slug:"natalia-v.-beloborodova",fullName:"Natalia V. Beloborodova",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/199461/images/system/199461.jpg",biography:'Natalia Vladimirovna Beloborodova was educated at the Pirogov Russian National Research Medical University, with a degree in pediatrics in 1980, a Ph.D. in 1987, and a specialization in Clinical Microbiology from First Moscow State Medical University in 2004. She has been a Professor since 1996. Currently, she is the Head of the Laboratory of Metabolism, a division of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russian Federation. N.V. Beloborodova has many years of clinical experience in the field of intensive care and surgery. She studies infectious complications and sepsis. She initiated a series of interdisciplinary clinical and experimental studies based on the concept of integrating human metabolism and its microbiota. Her scientific achievements are widely known: she is the recipient of the Marie E. Coates Award \\"Best lecturer-scientist\\" Gustafsson Fund, Karolinska Institutes, Stockholm, Sweden, and the International Sepsis Forum Award, Pasteur Institute, Paris, France (2014), etc. Professor N.V. Beloborodova wrote 210 papers, five books, 10 chapters and has edited four books.',institutionString:"Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology",institution:null},{id:"354260",title:"Ph.D.",name:"Tércio Elyan",middleName:"Azevedo",surname:"Azevedo Martins",slug:"tercio-elyan-azevedo-martins",fullName:"Tércio Elyan Azevedo Martins",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/354260/images/16241_n.jpg",biography:"Graduated in Pharmacy from the Federal University of Ceará with the modality in Industrial Pharmacy, Specialist in Production and Control of Medicines from the University of São Paulo (USP), Master in Pharmaceuticals and Medicines from the University of São Paulo (USP) and Doctor of Science in the program of Pharmaceuticals and Medicines by the University of São Paulo. Professor at Universidade Paulista (UNIP) in the areas of chemistry, cosmetology and trichology. Assistant Coordinator of the Higher Course in Aesthetic and Cosmetic Technology at Universidade Paulista Campus Chácara Santo Antônio. Experience in the Pharmacy area, with emphasis on Pharmacotechnics, Pharmaceutical Technology, Research and Development of Cosmetics, acting mainly on topics such as cosmetology, antioxidant activity, aesthetics, photoprotection, cyclodextrin and thermal analysis.",institutionString:null,institution:{name:"University of Sao Paulo",country:{name:"Brazil"}}},{id:"334285",title:"Ph.D. Student",name:"Sameer",middleName:"Kumar",surname:"Jagirdar",slug:"sameer-jagirdar",fullName:"Sameer Jagirdar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/334285/images/14691_n.jpg",biography:"I\\'m a graduate student at the center for biosystems science and engineering at the Indian Institute of Science, Bangalore, India. I am interested in studying host-pathogen interactions at the biomaterial interface.",institutionString:null,institution:{name:"Indian Institute of Science Bangalore",country:{name:"India"}}},{id:"329795",title:"Dr.",name:"Mohd Aftab",middleName:"Aftab",surname:"Siddiqui",slug:"mohd-aftab-siddiqui",fullName:"Mohd Aftab Siddiqui",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/329795/images/15648_n.jpg",biography:"Dr. Mohd Aftab Siddiqui is currently working as Assistant Professor in the Faculty of Pharmacy, Integral University, Lucknow for the last 6 years. He has completed his Doctor in Philosophy (Pharmacology) in 2020 from Integral University, Lucknow. He completed his Bachelor in Pharmacy in 2013 and Master in Pharmacy (Pharmacology) in 2015 from Integral University, Lucknow. He is the gold medalist in Bachelor and Master degree. He qualified GPAT -2013, GPAT -2014, and GPAT 2015. His area of research is Pharmacological screening of herbal drugs/ natural products in liver and cardiac diseases. He has guided many M. Pharm. research projects. He has many national and international publications.",institutionString:"Integral University",institution:null},{id:"255360",title:"Dr.",name:"Usama",middleName:null,surname:"Ahmad",slug:"usama-ahmad",fullName:"Usama Ahmad",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/255360/images/system/255360.png",biography:"Dr. Usama Ahmad holds a specialization in Pharmaceutics from Amity University, Lucknow, India. He received his Ph.D. degree from Integral University. Currently, he’s working as an Assistant Professor of Pharmaceutics in the Faculty of Pharmacy, Integral University. From 2013 to 2014 he worked on a research project funded by SERB-DST, Government of India. He has a rich publication record with more than 32 original articles published in reputed journals, 3 edited books, 5 book chapters, and a number of scientific articles published in ‘Ingredients South Asia Magazine’ and ‘QualPharma Magazine’. He is a member of the American Association for Cancer Research, International Association for the Study of Lung Cancer, and the British Society for Nanomedicine. Dr. Ahmad’s research focus is on the development of nanoformulations to facilitate the delivery of drugs that aim to provide practical solutions to current healthcare problems.",institutionString:"Integral University",institution:{name:"Integral University",country:{name:"India"}}},{id:"30568",title:"Prof.",name:"Madhu",middleName:null,surname:"Khullar",slug:"madhu-khullar",fullName:"Madhu Khullar",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/30568/images/system/30568.jpg",biography:"Dr. Madhu Khullar is a Professor of Experimental Medicine and Biotechnology at the Post Graduate Institute of Medical Education and Research, Chandigarh, India. She completed her Post Doctorate in hypertension research at the Henry Ford Hospital, Detroit, USA in 1985. She is an editor and reviewer of several international journals, and a fellow and member of several cardiovascular research societies. Dr. Khullar has a keen research interest in genetics of hypertension, and is currently studying pharmacogenetics of hypertension.",institutionString:"Post Graduate Institute of Medical Education and Research",institution:{name:"Post Graduate Institute of Medical Education and Research",country:{name:"India"}}},{id:"223233",title:"Prof.",name:"Xianquan",middleName:null,surname:"Zhan",slug:"xianquan-zhan",fullName:"Xianquan Zhan",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/223233/images/system/223233.png",biography:"Xianquan Zhan received his MD and Ph.D. in Preventive Medicine at West China University of Medical Sciences. He received his post-doctoral training in oncology and cancer proteomics at the Central South University, China, and the University of Tennessee Health Science Center (UTHSC), USA. He worked at UTHSC and the Cleveland Clinic in 2001–2012 and achieved the rank of associate professor at UTHSC. Currently, he is a full professor at Central South University and Shandong First Medical University, and an advisor to MS/PhD students and postdoctoral fellows. He is also a fellow of the Royal Society of Medicine and European Association for Predictive Preventive Personalized Medicine (EPMA), a national representative of EPMA, and a member of the American Society of Clinical Oncology (ASCO) and the American Association for the Advancement of Sciences (AAAS). He is also the editor in chief of International Journal of Chronic Diseases & Therapy, an associate editor of EPMA Journal, Frontiers in Endocrinology, and BMC Medical Genomics, and a guest editor of Mass Spectrometry Reviews, Frontiers in Endocrinology, EPMA Journal, and Oxidative Medicine and Cellular Longevity. He has published more than 148 articles, 28 book chapters, 6 books, and 2 US patents in the field of clinical proteomics and biomarkers.",institutionString:"Shandong First Medical University",institution:{name:"Affiliated Hospital of Shandong Academy of Medical Sciences",country:{name:"China"}}},{id:"297507",title:"Dr.",name:"Charles",middleName:"Elias",surname:"Assmann",slug:"charles-assmann",fullName:"Charles Assmann",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/297507/images/system/297507.jpg",biography:"Charles Elias Assmann is a biologist from Federal University of Santa Maria (UFSM, Brazil), who spent some time abroad at the Ludwig-Maximilians-Universität München (LMU, Germany). He has Masters Degree in Biochemistry (UFSM), and is currently a PhD student at Biochemistry at the Department of Biochemistry and Molecular Biology of the UFSM. His areas of expertise include: Biochemistry, Molecular Biology, Enzymology, Genetics and Toxicology. He is currently working on the following subjects: Aluminium toxicity, Neuroinflammation, Oxidative stress and Purinergic system. Since 2011 he has presented more than 80 abstracts in scientific proceedings of national and international meetings. Since 2014, he has published more than 20 peer reviewed papers (including 4 reviews, 3 in Portuguese) and 2 book chapters. He has also been a reviewer of international journals and ad hoc reviewer of scientific committees from Brazilian Universities.",institutionString:"Universidade Federal de Santa Maria",institution:{name:"Universidade Federal de Santa Maria",country:{name:"Brazil"}}},{id:"217850",title:"Dr.",name:"Margarete Dulce",middleName:null,surname:"Bagatini",slug:"margarete-dulce-bagatini",fullName:"Margarete Dulce Bagatini",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/217850/images/system/217850.jpeg",biography:"Dr. Margarete Dulce Bagatini is an associate professor at the Federal University of Fronteira Sul/Brazil. She has a degree in Pharmacy and a PhD in Biological Sciences: Toxicological Biochemistry. She is a member of the UFFS Research Advisory Committee\nand a member of the Biovitta Research Institute. She is currently:\nthe leader of the research group: Biological and Clinical Studies\nin Human Pathologies, professor of postgraduate program in\nBiochemistry at UFSC and postgraduate program in Science and Food Technology at\nUFFS. She has experience in the area of pharmacy and clinical analysis, acting mainly\non the following topics: oxidative stress, the purinergic system and human pathologies, being a reviewer of several international journals and books.",institutionString:"Universidade Federal da Fronteira Sul",institution:{name:"Universidade Federal da Fronteira Sul",country:{name:"Brazil"}}},{id:"226275",title:"Ph.D.",name:"Metin",middleName:null,surname:"Budak",slug:"metin-budak",fullName:"Metin Budak",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/226275/images/system/226275.jfif",biography:"Metin Budak, MSc, PhD is an Assistant Professor at Trakya University, Faculty of Medicine. He has been Head of the Molecular Research Lab at Prof. Mirko Tos Ear and Hearing Research Center since 2018. His specializations are biophysics, epigenetics, genetics, and methylation mechanisms. He has published around 25 peer-reviewed papers, 2 book chapters, and 28 abstracts. He is a member of the Clinical Research Ethics Committee and Quantification and Consideration Committee of Medicine Faculty. His research area is the role of methylation during gene transcription, chromatin packages DNA within the cell and DNA repair, replication, recombination, and gene transcription. His research focuses on how the cell overcomes chromatin structure and methylation to allow access to the underlying DNA and enable normal cellular function.",institutionString:"Trakya University",institution:{name:"Trakya University",country:{name:"Turkey"}}},{id:"243049",title:"Dr.",name:"Anca",middleName:null,surname:"Pantea Stoian",slug:"anca-pantea-stoian",fullName:"Anca Pantea Stoian",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/243049/images/system/243049.jpg",biography:"Anca Pantea Stoian is a specialist in diabetes, nutrition, and metabolic diseases as well as health food hygiene. She also has competency in general ultrasonography.\n\nShe is an associate professor in the Diabetes, Nutrition and Metabolic Diseases Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. She has been chief of the Hygiene Department, Faculty of Dentistry, at the same university since 2019. Her interests include micro and macrovascular complications in diabetes and new therapies. Her research activities focus on nutritional intervention in chronic pathology, as well as cardio-renal-metabolic risk assessment, and diabetes in cancer. She is currently engaged in developing new therapies and technological tools for screening, prevention, and patient education in diabetes. \n\nShe is a member of the European Association for the Study of Diabetes, Cardiometabolic Academy, CEDA, Romanian Society of Diabetes, Nutrition and Metabolic Diseases, Romanian Diabetes Federation, and Association for Renal Metabolic and Nutrition studies. She has authored or co-authored 160 papers in national and international peer-reviewed journals.",institutionString:null,institution:{name:"Carol Davila University of Medicine and Pharmacy",country:{name:"Romania"}}},{id:"279792",title:"Dr.",name:"João",middleName:null,surname:"Cotas",slug:"joao-cotas",fullName:"João Cotas",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279792/images/system/279792.jpg",biography:"Graduate and master in Biology from the University of Coimbra.\n\nI am a research fellow at the Macroalgae Laboratory Unit, in the MARE-UC – Marine and Environmental Sciences Centre of the University of Coimbra. My principal function is the collection, extraction and purification of macroalgae compounds, chemical and bioactive characterization of the compounds and algae extracts and development of new methodologies in marine biotechnology area. \nI am associated in two projects: one consists on discovery of natural compounds for oncobiology. The other project is the about the natural compounds/products for agricultural area.\n\nPublications:\nCotas, J.; Figueirinha, A.; Pereira, L.; Batista, T. 2018. An analysis of the effects of salinity on Fucus ceranoides (Ochrophyta, Phaeophyceae), in the Mondego River (Portugal). Journal of Oceanology and Limnology. in press. DOI: 10.1007/s00343-019-8111-3",institutionString:"Faculty of Sciences and Technology of University of Coimbra",institution:null},{id:"279788",title:"Dr.",name:"Leonel",middleName:null,surname:"Pereira",slug:"leonel-pereira",fullName:"Leonel Pereira",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/279788/images/system/279788.jpg",biography:"Leonel Pereira has an undergraduate degree in Biology, a Ph.D. in Biology (specialty in Cell Biology), and a Habilitation degree in Biosciences (specialization in Biotechnology) from the Faculty of Science and Technology, University of Coimbra, Portugal, where he is currently a professor. In addition to teaching at this university, he is an integrated researcher at the Marine and Environmental Sciences Center (MARE), Portugal. His interests include marine biodiversity (algae), marine biotechnology (algae bioactive compounds), and marine ecology (environmental assessment). Since 2008, he has been the author and editor of the electronic publication MACOI – Portuguese Seaweeds Website (www.seaweeds.uc.pt). He is also a member of the editorial boards of several scientific journals. Dr. Pereira has edited or authored more than 20 books, 100 journal articles, and 45 book chapters. He has given more than 100 lectures and oral communications at various national and international scientific events. He is the coordinator of several national and international research projects. In 1998, he received the Francisco de Holanda Award (Honorable Mention) and, more recently, the Mar Rei D. Carlos award (18th edition). He is also a winner of the 2016 CHOICE Award for an outstanding academic title for his book Edible Seaweeds of the World. In 2020, Dr. Pereira received an Honorable Mention for the Impact of International Publications from the Web of Science",institutionString:"University of Coimbra",institution:{name:"University of Coimbra",country:{name:"Portugal"}}},{id:"61946",title:"Dr.",name:"Carol",middleName:null,surname:"Bernstein",slug:"carol-bernstein",fullName:"Carol Bernstein",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/61946/images/system/61946.jpg",biography:"Carol Bernstein received her PhD in Genetics from the University of California (Davis). She was a faculty member at the University of Arizona College of Medicine for 43 years, retiring in 2011. Her research interests focus on DNA damage and its underlying role in sex, aging and in the early steps of initiation and progression to cancer. In her research, she had used organisms including bacteriophage T4, Neurospora crassa, Schizosaccharomyces pombe and mice, as well as human cells and tissues. She authored or co-authored more than 140 scientific publications, including articles in major peer reviewed journals, book chapters, invited reviews and one book.",institutionString:"University of Arizona",institution:{name:"University of Arizona",country:{name:"United States of America"}}},{id:"182258",title:"Dr.",name:"Ademar",middleName:"Pereira",surname:"Serra",slug:"ademar-serra",fullName:"Ademar Serra",position:null,profilePictureURL:"https://mts.intechopen.com/storage/users/182258/images/system/182258.jpeg",biography:"Dr. Serra studied Agronomy on Universidade Federal de Mato Grosso do Sul (UFMS) (2005). He received master degree in Agronomy, Crop Science (Soil fertility and plant nutrition) (2007) by Universidade Federal da Grande Dourados (UFGD), and PhD in agronomy (Soil fertility and plant nutrition) (2011) from Universidade Federal da Grande Dourados / Escola Superior de Agricultura Luiz de Queiroz (UFGD/ESALQ-USP). Dr. Serra is currently working at Brazilian Agricultural Research Corporation (EMBRAPA). His research focus is on mineral nutrition of plants, crop science and soil science. Dr. Serra\\'s current projects are soil organic matter, soil phosphorus fractions, compositional nutrient diagnosis (CND) and isometric log ratio (ilr) transformation in compositional data analysis.",institutionString:"Brazilian Agricultural Research Corporation",institution:{name:"Brazilian Agricultural Research Corporation",country:{name:"Brazil"}}}]}},subseries:{item:{id:"26",type:"subseries",title:"Machine Learning and Data Mining",keywords:"Intelligent Systems, Machine Learning, Data Science, Data Mining, Artificial Intelligence",scope:"The scope of machine learning and data mining is immense and is growing every day. It has become a massive part of our daily lives, making predictions based on experience, making this a fascinating area that solves problems that otherwise would not be possible or easy to solve. 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