Summary of results
In recent times, different strategies for thermal ablation therapy have been in use. They include radiofrequency ablation, cryoablation therapy, laser ablation therapy, microwave ablation and high intensity focused ultrasound ablation, among others.
This chapter focuses on the discussion of principles and application of the radiofrequency ablation therapy system as a minimally invasive treatment modality for hyperthermia therapy. Detailed work completed in the use of radiofrequency (RF) energy in cancer management by developing and testing an economical and effective thermal probe that will effectively destroy volumes of pathological tumours by means of hyperthermia is presented.
2. Radiofrequency energy and the RF ablation system
Basically, the term
2.1. Hyperthermic (thermal) coagulation necrosis
Coagulation necrosis denotes “irreversible thermal damage to cells even if the ultimate manifestations of cell death do not fulfill the strict histological criteria of coagulative necrosis” . The nature of the thermal damage caused by radiofrequency heating is dependent on both the tissue temperature achieved and the duration of heating. Here is what happens at various temperatures:
At 42oC, cells die but it may take a significant amount of time (approximately 60 min).
Between 42oC and 45oC, cells are more susceptible to damage by other agents like chemotherapy and radiation.
Over 46oC irreversible damage occurs depending on the duration of heating.
Between 50oC and 55oC, the duration necessary to shorten irreversible damage to cells is shortened to 4 – 6 minutes.
Between 50oC and 100oC there is near immediate coagulation of tissue, almost instantaneous protein denaturation, melting of lipid bilayers, irreversible damage to mitochondrial and cytosolic (key cellular) enzymes of the cells, DNA and RNA.
From 100oC to 110oC, tissue vaporizes and carbonizes, all of which decrease energy transmission and impede ablation.
Figure 2 shows tissue reaction to thermal injury at different temperatures . For successful ablation, the tissue temperature should be maintained in the ideal range (50 – 100oC) to ablate tumour adequately and avoid carbonization around the tip of the electrode due to excessive heating. For adequate destruction of tumour tissue, the entire volume of a lesion must be subjected to cytotoxic temperatures. Hence effective heating throughout the target volume (i.e. the tumour and about 5mm thickness around normal tissue) is required as shown in figure 3. Thus, the main objective of radiofrequency ablation therapy is to reach and maintain a temperature range of 50o – 100oC throughout the entire target volume for at least 4 – 6 minutes. However, the relatively slow thermal conduction from the electrode surface through the tissues increases the duration of application to 10 – 30 minutes.
Recommendations of heating for these extended durations are based on experimental and clinical data suggesting that thermal equilibrium and, hence, complete induction of coagulation are not achieved for a given radiofrequency application until these thresholds are achieved. The development of a radiofrequency ablation system in this study is aimed at producing a device that is able to satisfy the minimum requirement for effective tumour ablation at the ideal (cytotoxic) ablation temperature.
2.2. Principles of radiofrequency ablation
Radiofrequency ablation is physically based on radiofrequency current (about 460 kHz) that passes through the target tissue from the tip of an active electrode (RF thermal probe) towards a dispersive electrode which serves as the grounding pad. These two electrodes are connected to a radiofrequency generator. The active electrode has a very small cross-sectional area (a few square millimetres) with respect to the passive electrode. The active electrode is usually fashioned into the form of a needle-like probe that is inserted into the tumour. The dispersive electrode has a much larger area than the active electrode, on the order of 100cm2 or larger, and is usually placed firmly behind the right shoulder or the thigh of the subject, depending on the location of the tumour in the body. Current flowing into the dispersive electrode is the same as the current flowing into the active electrode. But since the active electrode has a far smaller cross-sectional area than the dispersive electrode, the current density in amperes per square meter (A/m2) is far greater. As a result of the difference in current density between the two electrodes, the energy at the tip of the probe leads to ionic agitation with subsequent conversion of friction into heat. The tissue ions are agitated as they attempt to follow the changes in direction of alternating electric current as shown in figure 4 below.
The agitation results in frictional heat around the electrode. The marked discrepancy between the surface area of the needle electrode and the dispersive electrode causes the generated heat to be tightly focused and concentrated around the needle electrode. The use of a large grounding pad ensures maximum surface area for dispersion of current from the needle electrode. The grounding pad also maximizes dispersion of equal amounts of energy and heat at the grounding pad sites, thereby minimizes the risk of burns. The tissue underneath the passive electrode heats up only slightly, while the tissue in contact with the active electrode is resistively heated to elevated temperatures sufficient for tumour ablation (coagulative necrosis). The strategy of RF ablation is to create a closed-loop circuit including the RF generator, the needle electrode, the patient (tissue) and the passive electrode (grounding pad) in series. The heating of tissue is due to the power dissipated in the tissue, which is found from the expression
Appreciable advances have been made over the past decade to produce application devices for RFA. The
3. Materials and methods
The RF thermal probe developed was designed on a SolidWorks platform and manufactured according to design specifications. The device consists of an RF shielded insulated handle with a needle probe. The shaft of the needle is also insulated except for the tip which makes physical contact with the tumour or volume to be treated. A coaxial cable connects the device to the RF power unit. The RF thermal probe uses a stainless steel needle (size 14G x 3-1/4) with a diameter and length of 2.1 x 80 mm, connected to the conducting coaxial cable in one end, and housed in an epoxy resin holder (probe handle) that is 120 mm long and 15 mm in diameter. The stainless steel needle is insulated, except for the exposed 20 mm tip that makes direct contact with tissue. The insulation prevents normal tissue from being destroyed along with cancerous tissue during thermal ablation treatment. The probe (as shown in figure 5) is reusable and is made of epoxy-resin material that can be easily steam-cleaned.
An essential objective of radiofrequency ablation therapy is to achieve and maintain a temperature range of 50 – 100oC throughout the entire target volume for at least 4 – 6 minutes [23-25]. From equation (1), power dissipated (P) is directly proportional to volume (V). Tumour is usually treated as a sphere, and volume of a sphere is given by,
To verify that the radiofrequency thermal probe developed is a device that is able to satisfy this minimum requirement for effective tumour ablation at the ideal cytotoxic temperature, experimental tests were done with different tissues types to determine how each tissue type responds to RF energy by observing and recording the temperature change at the probe tip. Liver, lung, brain, kidney and soft tissue were tested at different power settings to determine which power setting gives the best results with each tissue type in terms of the minimum time to reach the ideal temperature range, and the maximum time to remain within this range without charring or vapourizing. An RF generator (460 KHz) was connected in a closed circuit with the RF thermal probe, tissue sample, and dispersive electrode in series. Each tissue type was tested with different power settings, and each test was done for about 15 minutes.
4. Results and discussion
The extent of coagulation necrosis is dependent on the energy deposited, local tissue interaction minus the heat lost.
Heat efficacy is defined as the difference between the amount of heat produced and the amount of heat lost. Therefore, effective ablation can be achieved by optimizing heat production and minimizing heat loss within the area to be ablated. The relationship between these factors has been well characterized as the “bio-heat equation.” Heat production is correlated with the intensity and duration of the radio-frequency energy deposited. Heat conduction or diffusion is usually explained as a factor of heat loss in regard to the electrode tip. Heat is lost mainly through convection by means of blood circulation. Therefore, the cooling tissue by perfusion can limit the reproducible size of the ablation lesion in vivo.
Macroscopic and microscopic examination of tissue samples tested show clear evidence of coagulation necrosis. A tissue volume of up to 20 mm diameter was necrosed with the single-tine probe developed. The plots of temperature versus time for different tissue types tested using different power settings are presented in the following figures:
From figure 6, it is seen that, while 1 watt was inadequate for coagulation necrosis in lung tissue, 3 watts showed evidence of carbonization, leading to a drop in temperature as further conduction is inhibited. The best result was achieved with 2 watts, which showed a steady rise in temperature maintained within the ideal ablation temperature range.
The plot in figure 7 shows that, while 2 watts was below the ideal temperature range, and therefore inadequate for effective tissue necrosis, 4 watts was too high and showed evidence of carbonization, resulting in a drop in temperature due to inhibition in conduction. The best result in terms of effective tissue necrosis was achieved with the 3 watts power setting.
In figure 8, the plot shows that, while 5 watts and 6 watts are within the ideal ablation zone, the 7 watts setting is too high for liver tissue as it produced carbonization resulting in temperature drop. The best result however was recorded with the 6 watts setting which shows a steady rise in temperature without carbonization or charring.
In figure 9, the results show that 15 watts produced temperature above the ideal ablation range, leading to carbonization and consequently a drop in temperature. Both 10 watts and 13 watts are suitable for ablating kidney tissue as seen, with 13 watts giving the best results since it allows the use of a higher temperature.
In figure 10, the results show that, while 15 watts produced temperature within the ideal ablation range, the best result was obtained with the 20 watts power setting since the temperature is higher. This means that the ideal temperature range for treatment will be reached quicker with 20 watts. The 10 watts power setting produces temperature below the ideal range, and was therefore inadequate for ablating soft tissue.
5. Summary of results
The above results have been summarized in table 2 below showing the different tissue types, the best power settings suitable for each tissue type, the minimum and maximum time required to keep the temperature within the ideal ablation range of 50 to 1000C, and the total duration. The total duration is the difference between the maximum time and minimum time.
|Tissue Type||Power (w)||Min. Time (min)||Max. Time (min)||Duration (min)|
The search for less morbid and less invasive techniques for cancer treatment has led to a strong drive within the global oncology community to develop and implement even more minimally invasive diagnostic and therapeutic procedures. The goals of these minimally invasive therapies for the treatment of cancerous tumours that have made them attractive to both patients and physicians are summarized as:
Viable and effective treatment options and eradicate in situ local disease
They are minimally invasive and less traumatic
Real-time imaging guidance is possible
Most procedures require only local anaesthesia, and recovery time is faster
Procedure can be repeated in case of cancer recurrence
They are safer, with minimal side effects, and limit postoperative morbidities and mortality
Real-time imaging guidance is possible
Non-surgical candidates can benefit from these treatment modalities
They can be performed as an outpatient procedure, or with only a short hospital stay
Shorten the time to return to daily function and work, and
Their low-cost make them cheaper and ultimately reduce the overall cost of cancer treatment.
With several ablation techniques available, the ablation characteristics and method of application will differentiate one ablation method from another. Though RF ablation is more widely used, it has its limitations. Most thermo-ablative procedures could be performed in the doctor’s office as an outpatient procedure with mild or no sedation. With RF ablation, it requires an increase in temperature to induce necrosis (tissue death). The heat needed for this necrosis requires that large amount of local anaesthetic be infused around the treatment site. This excess fluid blurs the ultrasound visualization of RFA and other heat-based ablation techniques. Though all the available literature agree that thermal ablation therapy is relatively safe and much less traumatic than radical surgical procedures, some complications and side effects have been reported. Some of these complications and side effects have been associated with probe design, probe placement, or the use of multiple probes. The use of both single and multiple probe placements have been described in many studies. Both have their advantages and disadvantages. Though multiple probes appear to be more successful in destroying larger tissue volumes, their use increases the risk of complications in the procedure. Finally, the high cost of RF ablation equipment, coupled with their limited availability has placed these treatment procedures above the reach of most patients and physicians in Sub-Saharan Africa. This project work which aims to investigate the design and development of a minimally invasive thermo-active oncology probe, will narrow this price gap and make treatment more affordable, and readily available to the ordinary patient in Sub-Saharan Africa.