The era of cancer chemotherapy began in the 1940s with the first use of nitrogen mustards and antifolate drugs. The practice of cancer medicine has changed dramatically allowing treatments for many previously fatal cancers. Furthermore, the adjuvant chemotherapy and hormonal therapy can extend life and prevent disease recurrence following surgical resection of diferent types of malignancies.
Concurrently with the new discoveries of chemotherapeutic agents, the remarkable scientific and technological development allowed understanding of cell biology of human cancer cells and thereby the emergence of targeted therapy. Although the targeted therapy drugs have had outstanding successes in selected types of cancer, new therapies are not likely to replace cytotoxic agents in the foreseeable future. Rather, clinical trials have demonstrated potent synergy between targeted molecules and traditional cytotoxic agents.
The compounds used in cancer therapy are quite varied in structure and mechanism of action, including: alkylating agents, antimetabolite analogs, natural products, hormones and hormone antagonists and a variety of agents directed at specific molecular targets.
In this chapter we will discuss the history, applications and toxicity, among other aspects, of these agents that, in spite of systemic toxicity and severe side effects, became a mainstay of cancer treatment. As molecularly targeted agents have been used on a quite widespread way among different cancers, it can already be considered a conventional cancer therapy. Even more, as a full chapter of this book will be dedicated to these agents this subject will not be discussed in the present chapter.
2. Alkylating agents
Alkylating agents are genotoxic drugs which affect the nucleic acids and their function by direct binding to the DNA, interfering with replication and transcription resulting in mutations. In this way, the goal of using these agents is to induce DNA damage in cancer cells, severe enough to provoke them to enter into apoptosis. Alkylating agents act by replacing a hydrogen atom into another molecule by an alkyl radical through the electrolytic attack by the alkylating agent; however, this compound can also react with molecules containing an atom in a lower valence state that will undergo electrolytic attack instead of hydrogen.
Alkylating agents can be divided in several subgroups which include nitrogen mustards, various alkylating agents and platinum coordination complexes. Each one of these groups will be discussed below.
2.1. Nitrogen mustards
Nitrogen mustards were the first clinically useful anticancer agents  and its derivatives, such as cyclophosphamide, are still among the most widely used antitumor drugs .
Cyclophosphamide is a derivative of nitrogen mustards with a modified chemical structure that confers it a greater specificity for cancer cells . The rational on developing cyclophosphamide was that cancer cells express higher levels of phosphamidase, which is able to cleave the phosphorus-nitrogen (P-N) bond, releasing the nitrogen mustard within the cancer cell ; this premise was later proven inaccurate . The first clinical trials with cyclophosphamide occurred in 1958, when this drug was found to be the most effective anticancer compound against 33 cancer types on a 1,000 compounds screening trial . In 1959, cyclophosphamide was approved by the Food and Drug Administration (FDA) as a cytotoxic anticancer compound, and up until now, over 50 years of its approval, it is still one of the most successful anticancer drugs . Cyclophosphamide is used for the treatment of lymphoma, leukemias, breast and ovary cancers [7-10].
Cyclophosphamide is administered as a prodrug which is highly stable and requires hepatic mixed function oxidase system to be metabolically activated. Hepatic cytochrome P-450 systems are responsible for generating 4-hydroxycyclophosphamide by the hydroxylation of the oxazaphosphorine ring on cyclophosphamide. Several metabolites are generated but 4-hydroxycyclophosphamide is considered the most significant as it distributes throughout the body, including reaching the tumor where it is preferentially converted into the active nitrogen mustard as described above .
Afterwards, the active nitrogen mustard will form adducts in the DNA in a sequential alkylation process in which each drug molecule will react with two different nucleotides: firstly it forms a monofunctional adduct followed by a second adduct on the opposite strand of the DNA, forming an interstrand cross-link. This cross-link will prevent strands from separating during replication, inhibiting DNA synthesis .
Iphosphamide is chemically related to cyclophosphamide by transposition of a chloroethyl group from the exocyclic to endocyclic nitrogen. Clinical investigations have highlighted the lower toxicity of iphosphamide in comparison to that observed for cyclophosphamide . Doxorubicin and iphosphamide remain the backbone of chemotherapy in patients with locally advanced or metastatic soft tissue sarcoma . In the mid 1980s, iphosphamide was found to be effective in patients with refractory germ cell tumors .
2.2. Diverse alkylating agents
Nitrosoureas were synthesized at the National Cancer Institute (NCI) following rational design based on structure-activity relation . Nitrosoureas can react through alkylation with both nucleic acids and proteins or specifically through carbamylation with the latter. In order to acquire its alkylating and carbamylating properties these compounds undergo a nonenzymatic decomposition to form a 2‑chloroethyl carbonium ion, which is highly electrophile and capable of alkylating guanine, cytidine, and adenine. Some compounds of this drug category are: (i) 2‑chloroethylnitrosoureas (
Triazene compounds of clinical interest, dacarbazine and temozolomide, are a group of alkylating agents with similar chemical, physical, antitumor and mutagenic properties. Their mechanism of action is mainly related to methylation of
Temozolomide is an oral alkylating agent with established antitumor activity in patients with melanoma and primary brain tumors, due to its excellent bioavailability in the central nervous system . Dacarbazine is employed to treat Hodgkin disease and malignant melanoma [19,20].
2.3. Platinum complexes
Rosenberg and colleagues reported, in 1965 the discovery that platinum complexes present in nutrient medium in low concentrations can inhibit cell division of
Since then, over 3,000 platinum derivatives have been synthesized and tested against cancer cells . Because of renal, oto and neurotoxicities of cisplatin, there were intensive efforts to devise analogs with fewer of these serious side effects. Moreover, analogs of cisplatin have been developed in an attempt not only to lessen the toxicity of the parent compound, but also to try to overcome the problem of platinum resistance, which may be present either in the outset of the disease, or emerging during its course or yet be acquired as a result of the treatment . This effort led to the development of carboplatin, which produces primarily hematopoietic toxicity and has antitumor effects similar to those of cisplatin. Other platinum compounds have been developed and evaluated, as described later, although they haven’t showed any significant advantages over cisplatin and carboplatin .
Today, six platinum compounds are used clinically: cisplatin (available since 1978); carboplatin and oxaliplatin (world-wide 2nd generation analogs); nedaplatin (also a 2nd generation analog); and lobaplatin and heptaplatin (3rd generation analogs). Some platinum complexes are still under clinical investigation, including those developed for oral administration.
Cytotoxicity of platinum compounds is believed to result from the formation of platinum-DNA adducts . In fact, these platinum drugs can be considered as prodrugs, yielding after aquation the active diaquo-platinum compound. The main differences between these prodrugs can be related to the different kinetics of activation. Hydrolysis of cisplatin is extremely rapid, whereas it is slower for carboplatin and nedaplatin. The diaquo-platinum species react with the amine groups of proteins, RNA and DNA. The latter reaction yields platinum-DNA adducts, which appears to be associated with antitumor activity. Aquated platinum reacts preferentially with the N-7 position of guanine and adenine and produces cross-links between bases in the same strand (intrastrand) or opposite strands (interstrand). The efficacy of platinum agents against cancer cells may be mediated with the inhibition of DNA synthesis or saturation of the cellular capacity to repair platinum adducts on DNA .
Cisplatin is used alone and in combination with a wide range of other drugs. In combination with bleomycin, etoposide, iphosphamide, or vinblastine, cures 90% of patients with testicular cancer. Cisplatin or carboplatin used with paclitaxel induces complete response in the majority of patients with ovary carcinoma. Cisplatin produces responses in bladder, head and neck, cervix, and endometrium cancers; all forms of lung carcinoma; anal and rectal carcinomas; and neoplasms of childhood [27,25].
Resistance and the spectrum of clinical activity of carboplatin are similar to those of cisplatin. Carboplatin is relatively well tolerated clinically, causing less nausea, neurotoxicity, ototoxicity, and nephrotoxicity than cisplatin. Instead, the dose-limiting toxicity is myelosuppression, primarily thrombocytopenia .
Oxaliplatin exhibits a variety of antitumor activity such as against colorectal and gastric cancers which differs from other platinum agents . A great number of phase II and III trials in solid tumors administering oxaliplatin in combination with other drugs have suggested increased activity as compared to oxaliplatin alone. Further, in comparison to cisplatin, oxaliplatin has not demonstrated nephrotoxic effects, which is due to the absence of platinum accumulation in plasma .
Antimetabolites are cytotoxic agents developed for more than 65 years and considered a mainstay in cancer chemotherapy. The antimetabolites can be divided according with their structure and function as folic acid analogs, purine analogs, pyrimidine analogs and cytidine analogs.
These agents are structurally similar to natural metabolites, which are essential for normal biochemical reactions in cells. The mechanism of action of antimetabolites include: competition for binding sites of enzymes that participate in essential biosynthetic processes and incorporation into nucleic acids, which inhibits their normal function triggering the apoptosis process.
3.1. Folic acids analogs
In 1948, aminopterin, an antifolate drug, was the first drug to induce temporary remissions in children with acute lymphoblastic leukemia (ALL) . This success stimulated research into new antimetabolites less toxic than aminopterin. A few years later another antifolate drug, methotrexate (MTX) showed anticancer property. Currently, this drug category plays an important role in cancer treatment acting in several ways. Mostly they compete with folates for uptake into cells and prevent the formation of folates coenzymes primarily by inhibiting dihydrofolate reductase (DHFR) or thymidylate synthase (TS).
Mammalian cells lack the ability to synthesize their own reduced folate derivatives and therefore must obtain them from exogenous sources (i.e. food and dietary supplements). In normal and cancer cells, folic acid is reduced to dihydrofolate (FH2) and then to active tetrahydrofolate (FH4) by the enzyme DHFR. FH4 is a cofactor that provides methyl groups for the synthesis of precursors of DNA (thymidylate and purines) and RNA (purines). TS catalyses transfer of the carbon from the FH4 to the target molecules by oxidizing the folate ring of the FH4, reverting it back into a FH2 .
Although several antifolate drugs have been developed, MTX is the antifolate with the widest spectrum of use. MTX is extensively used in lymphoma, ALL and osteosarcoma. Moreover, MTX is part of chemotherapeutic schemes for choriocarcinoma, breast, bladder and head and neck cancer .
MTX enters the cells via reduced folate carrier (RFC) or via the membrane folate binding protein (FBP) and is polyglutamated by folylpolyglutamate synthetase (FPGS) in MTX-polyglutamate, which is retained in cells for longer periods compared with MTX [31,32]. The main target of MTX and MTX-polyglutamate is the inhibition of DHFR enzyme, leading to partial depletion of the FH4 cofactors required for the synthesis of new thymidylate and purines nucleotides. Consequently, there will be a decrease of DNA and RNA synthesis. In addition, MTX-polyglutamates are also inhibitors of other folate-requiring enzymes such as: TS and two enzymes related with de novo purine synthesis - glycinamide ribonucleotide transformylase (GART) and aminoimidazole carboxamide ribonucleotide transformylase (AICART) .
In normal cells a decreased polyglutamation is observed when compared to malignant cells, which partially explains the selectivity of MTX for malignant tissue . Despite this predilection for malignant cells, MTX can kill rapidly dividing normal cells such as those of the intestinal epithelium and bone marrow . Common side effects are cytopenia, serious infections, liver damage, mucocutaneous problems, alopecia and allergic interstitial pneumonitis .
Pemetrexed is a multitargeted antifolate chemotherapy agent approved by FDA in 2004 for the treatment of malignant pleural mesothelioma (MPM) and advanced or metastatic non-small cell lung cancer (NSCLC). Ongoing clinical trials are evaluating pemetrexed efficacy in other malignancies such as breast, colorectal, bladder, cervical, gastric and pancreatic cancer .
Likewise MTX, pemetrexed inhibits DHFR and as a polyglutamate, it inhibits even more potently GART and TS . The inhibition of TS and GART predominates because pemetrexed’s usage produces little changes in the pool of reduced folates.
Currently, this agent is employed as a monotherapy or in combination with cisplatin. It is generally a well-tolerated drug and the most common adverse reactions with its usage as single-agent are fatigue, nausea, and anorexia. Myelosuppression is the most common and dose-limiting toxicity, predominantly developed as neutropenia .
3.2. Purine analogs
Purine nucleoside analogues (PNA) were identified for the first time by Hitchings and Elion in 1942 with antileukemic and immunosuppressant properties . The 6-mercaptopurine (6-MP) is the oldest PNA approved for clinical use, employed in the treatment of acute leukemias. The next generation of PNAs has been available worldwide since the 1990s, comprising primarily the cladribine, pentostatin, and fludarabine. PNAs have an important role as chemotherapeutic agents in hematological malignancies .
The 6-mercaptopurine (6-MP) was one of the first chemotherapeutic agents to be used in acute leukemia, remaining up today as one of the most useful drugs in acute leukemia’s treatment [41,42]. In 1953 FDA approved the usage of 6-MP after a short 2 years mean time period of its synthesis. At this time there were only MTX and steroids as established treatment options for ALL, the commonest childhood cancer .
This chemotherapeutic agent is a prodrug, analogue of hypoxanthine, a naturally occurring purine derivative. 6-MP requires intracellular conversion into 6-thioinosine-5'-monophosphate (TIMP) by the hypoxanthine guanine phosphoribosyl transferase (HGPRT). TIMP is a substrate of thiopurine S-methyltransferase (TPMT) producing methylated TIMP which is an effective inhibitor of de novo purine biosynthesis. The TIMP that is not involved in catabolism is further metabolized by inosine monophosphate dehydrogenase (IMPDH) and later metabolized by a series of kinases and reductases to produce deoxy-6-thioguanosine5’triphosphate (thio-dGTP). Incorporation of thio-dGTP has been shown to trigger cell-cycle arrest and apoptosis involving the DNA mismatch repair .
Fludarabine phosphate (FAMP) has activity in various indolent B cell malignancies and it was approved in 1991 for clinical usage in the treatment of chronic lymphocytic leukemia (CLL). FAMP is a prodrug that requires metabolic conversion to exert cytotoxic activity. It is rapidly dephosphorylated to 9-β-D-arabinosyl-2-fluoroadenine (F-ara-A), transported into cells and then phosphorylated by deoxycytidine kinase to the active form 2-fluoro-ara-ATP (F-ara-ATP) [45,46]. The F-ara-ATP is the only metabolite of FAMP that have cytotoxic activity, acting through different mechanisms that affect DNA synthesis.
F-ara-ATP inhibits ribonucleotide reductase (RNR), responsible for the conversion of ribonucleotides into deoxyribonucleotides which in turn are one of the key components at the construction of DNA strands. Furthermore F-ara-ATP incorporates into DNA, at the 3’-terminus, resulting in repression of DNA polymerization as well as inhibition of DNA ligase, an enzyme involved in DNA replication  and DNA primase, an accessory protein that synthesizes an RNA primer required for initiation of synthesis by DNA polymerase .
The most frequent adverse events associated with FAMP regimens are myelosuppression lymphocytopenia and infection, typically on respiratory tract. Despite the minor occurrence, severe neurotoxicity is one of the complications associated with FAMP .
Likewise FAMP, cladribine (2-CdA; 2-chloro-2'-deoxyadenosine) is phosphorylated and accumulated as 2-chlorodeoxyadenosine triphosphate (2-CdA-TP) in cells . This metabolite disrupts cell metabolism by incorporating into the DNA then inhibits DNA synthesis and repair, leading to accumulation of DNA strand breaks . In addiction 2-CdA-TP is a potent inhibitor of RNR.
2-CdA was shown to have potent and long-term effects in the treatment of low-grade B-cell neoplasms, approved by FDA for clinical use in hairy cell leukemia (HCL). It shares the same adverse effects of FAMP, being the bone marrow suppression its major toxic effect, associated with severe infections.
Pentostatin (deoxycoformycin; DCF) is a natural product first isolated from the culture of
The primary site of action is the inhibition of adenosine deaminase (ADA), an enzyme that participates in purine salvage metabolic pathways. Inhibition of ADA leads to accumulation of adenosine and deoxyadenosine nucleotides in cells, which can block DNA synthesis by inhibiting RNR. Another important action of pentostatin is the inactivation of S-adenosyl homocysteine hydrolase by deoxyadenosine, resulting in accumulation of S-adenosyl homocysteine, an intermediate in the synthesis of cysteine and adenosine particularly toxic to lymphocytes. Pentostatin also has adverse effects related with the bone marrow suppression.
3.3. Pyrimidine analogs
Pyrimidine analogs sparked the interest of scientists from the observation that rat malignant tissue used pyrimidine uracil more rapidly than normal tissues . In the late 1950s Charles Heidelberger and colleagues synthesized the fluoropyrimidine 5-fluorouracil (5-FU) , which demonstrated speciﬁc uracil antagonism within antitumor capabilities. Others pyrimidine analogs were developed later (e.g. capecitabine, cytosine arabinoside and gemcitabine) and this class is currently extensively used in cancer therapy.
5-FU is the mainstay of treatment for many common malignant diseases, particularly for colorectal cancer. It’s also used in breast, pancreatic and head and neck cancers . This antimetabolite exerts its antitumor effects through several mechanisms including inhibition of the enzyme TS, related to thymidine synthesis from uridine, and incorporation of its metabolites into RNA and DNA. 5-FU enters into cells rapidly and is converted intracellularly by metabolic enzymes into its active metabolite 5-fluoro-2’-deoxyuridine-5’-monophosphate (FdUMP). FdUMP inhibits TS leading to nucleotide pool imbalance, decreasing thymine concentration which leads to uracil incorporation into DNA causing DNA strand breaks . Another 5-FU metabolite, fluorouridine triphosphate (FUTP) is extensively incorporated into RNA, disrupting normal RNA processing and function.
Usual side effects of 5-FU are gastrointestinal, including nausea, vomiting, diarrhea, and stomatitis. Hematologic effects are also common, like myelosuppression and thrombocytopenia. Although considered unusual, cardiotoxicity has been reported as well as adverse neurological effects [54,55].
Capecitabine (N4-pentyloxycarbonyl-5’-deoxy-5-fuorocytidine) is an oral prodrug of 5-FU developed with the objective of improving tolerability and intratumor drug concentrations through tumor-specific conversion to the active drug. This selectivity is due the presence at higher levels of thymidine phosphorylase (TP), the final enzyme responsible for conversion to the active drug, in cancer cells .
In 1998, capecitabine was approved by FDA for the treatment of women with taxane- and anthracycline-refractory advanced breast cancer. Afterwards, this antimetabolite received the approval for metastatic colorectal carcinoma.
The most common toxicities related to treatment with capecitabine are gastrointestinal effects (diarrhea, nausea and vomiting) and hand-foot syndrome . Other adverse effects were also observed such as alopecia, myelosuppression and cardiotoxicity [57,55].
The deoxycytidine analogue gemcitabine (difluorodeoxycytidine, dFdC) received its first approval by FDA in 1996 for the treatment of patients with pancreatic cancer and NSCLC. Furthermore, gemcitabine was approved for the first-line treatment of patients with metastatic breast cancer and advanced ovarian cancer, in 2004 and 2006, respectively.
Gemcitabine is phosphorylated by deoxycytidine kinase (DCK) to its 5’-monophosphate form (dFdCMP) and additionally metabolized by several other enzymes to its 5’-diphosphate (dFdCDP) and 5’-triphosphate derivatives (dFdCTP). Then, this last metabolite dFdCTP is incorporated into DNA, inhibiting DNA replication and inducing apoptosis .
The major dose-limiting toxicity of gemcitabine is myelosuppression, but other adverse effects are related with the therapy such as flu-like symptoms, nausea, vomiting and rash .
Cytarabine (1-β-D-arabinofuranosylcytosine; ara-C) had its first approval by FDA in 1969 as a chemotherapeutic agent to be used with other drugs for the treatment of adult and pediatric acute myelogenous leukemia (AML). According to the NCI, cytarabine is also approved to treat ALL, chronic myelogenous leukemia (CML) and as a single agent to prevent and treat meningeal leukemia.
Since its approval by FDA in 1969, the clinical effectiveness of this drug has increased with knowledge of its pharmacologic and biologic properties. Ara-C is a prodrug and needs to be converted to its active form, ara-C 5’-triphosphate (Ara-CTP), by a series of intracellular enzyme-dependent phosphorylation steps .
Its mechanism of action is similar to that of the deoxycytidine analogue Gemcitabine: Ara-C is transported into the cell and once it is inside, it is phosphorylated into ara-C monophosphate (ara-CMP) by DCK and eventually to ara-C triphosphate (ara-CTP) which then competes with deoxycytidine triphosphate (dCTP) for incorporation into DNA and subsequently blocking DNA synthesis causing cell death .
Treatment with ara-C is associated with several adverse side effects, including myelosuppression (mostly leukopenia, thrombocytopenia and severe anemia), infections, mucositis, neurotoxicity, and acute pulmonary syndrome [61,27]
4. Microtubule-target agents
Microtubules are dynamic structures composed of α–β-tubulin heterodimers and microtubule-associated proteins representing one of the major components of the cytoskeleton. Microtubules are involved in many cellular processes including maintenance of cell structure, protein transportation and mitosis. Because of the central role of microtubules in mitosis, drugs that affect microtubule are useful in cancer chemotherapy. In this context, Microtubule-Targeted Agents (MTAs) constitute a class of anticancer drugs largely used in the clinics to treat solid tumors and hematological malignancies, either alone or as part of different combination regimens. MTA are potent mitotic poisons that are broadly classified into microtubule-stabilizing (e.g. taxanes and epothilones) and microtubule-destabilizing (e.g. vinca alkaloids) drugs.
4.1. Vinca alkaloids
The first natural anticancer agents approved to clinical use were the vinca alkaloids vincristine and vinblastine, introduced in the late 1960s. Vinca alkaloids were originally isolated from the Madagascar periwinkle
Vincas are classified as destabilizing agents due to their ability to cause microtubule depolymerization, suppress treadmilling and dynamic instability, blocking mitotic progression, and ultimately result in cell death by apoptosis. Vinca akaloids bind in one of three sites on tubulin, called the “vinca” domain, located near the exchangeable GTP binding site [63-65].
Vinca alkaloids differ in their chemotherapeutic effectiveness being part of therapeutic schemes in different types of malignancies. Vincristine is used in combination chemotherapy for treating pediatric leukemias, Hodgkin and non-Hodgkin lymphoma, as well as solid tumors such as Wilms tumor and neuroblastoma [66-68]. Vincristine can occasionally be used in the treatment of small cell lung cancer (SCLC). Currently, vinblastine is a standard component of regimens for treating lymphomas including Hodgkin’s disease. It´s also used for the treatment of bladder cancer, testicular carcinomas, germ cell malignancies and breast cancer [66,69]. Moreover the semisynthetic derivate of vinblastine, vinorelbine, has activity against NSCLC and breast cancer [70,71].
Furthermore these compounds diverge in their toxicities. While severe neurotoxicity is observed less frequently with vinorelbine and vinblastine, this side effect is frequently noticed with vincristine [72,73]. Myelosuppression, in turn, predominates with vinblastine and vinorelbine  and is the main dose-limiting toxicity of those drugs.
Vinca alkaloids and the others MTAs can present resistance in cancer cells due to: (i) cellular efflux of the anticancer agents, especially by the overexpression of drug efflux pumps, such as P-glycoprotein and multidrug resistance-associated protein 1 (MRP1) ; (ii) mutations in tubulin at the drug binding sites [76,77]; (iii) changes in the tubulin isotype composition of microtubules  and; (iv) changes in micro-tubule-regulatory proteins .
Taxanes are natural cytotoxic diterpenes classified as microtubule-stabilizing anticancer agents. Paclitaxel and the semisynthetic analog docetaxel are considered to be among the most important anticancer drugs in cancer chemotherapy.
Paclitaxel was identified in 1971 as part of a NCI program that screened medicinal plants for potential anticancer activity, whereof the researchers found cytotoxic effects on solid tumors and leukemic cells . Paclitaxel was initially derived from the bark of the Pacific yew (
Docetaxel, in turn, is an esterified derivative of 10-deacetylbaccatin-III, produced by Potier and his colleagues in 1986 . The structures of paclitaxel and docetaxel differ on the ester side chain attached at C-13 and in substitutions at the C-10 taxane ring position, which confers docetaxel slightly more water solubility than paclitaxel [83,25]
These drugs interact with β-tubulin promoting tubulin polymerization and formation of stable microtubules, even in the absence of GTP- and microtubule-associated proteins, which are usually essential for these processes. This inhibition of microtubule depolymerization results in mitotic arrest leading to apoptosis of the cancer cells . Furthermore, taxanes have been demonstrated to induce many other cellular effects that may or may not relate to their disruptive effects on microtubule dynamics, including the directly phosphorylation, hence inactivation, of proteins that blocks apoptosis in cancer cells (such as bcl-2) .
Paclitaxel was approved by the FDA in 1992 for the treatment of refractory breast cancer and refractory ovarian cancer. Currently this agent has a central role in the treatment of breast, ovarian, NSCLC and AIDS-related Kaposi’s sarcoma. In turn, docetaxel received the approval in 1995 for the treatment of metastatic breast cancer. Furthermore was approved for use in hormone refractory prostate cancer (HRPC), advanced squamous cell carcinoma of the head and neck, breast cancer, gastric adenocarcinoma and NSCLC.
Treatment with these drugs often results in a number of undesirable side effects, as well as resistance in cancer cells, as mentioned previously. In order to overcome those problems, novel taxanes are in development as well as novel formulations. In 2005 Abraxane® (paclitaxel albumin-bound nanoparticles, solvent-free) was approved for advanced breast cancer. Abraxane® prevent the hypersensitivity reactions typically associated with paclitaxel, which are generally related to the solvent suspension of polyoxyethylated castor oil (Cremophor EL) [86-88].
Taxanes exerts its primary toxic effects on the bone marrow, mainly neutropenia, and may cause neuropathy . Docetaxel causes greater degrees of neutropenia than paclitaxel. Furthermore, docetaxel can cause fluid retention leading to peripheral edema and pulmonary edema, in extreme cases. Despite the high incidence of major hypersensitivity reactions due to the Cremophor EL vehicle, these reactions are no longer a serious problem due to the advent of effective premedication regimens  and new formulations .
Epothilones are a new class of natural cytotoxic antineoplastic microtubule-stabilizing agents. Ixabepilone, a semisynthetic analog of the natural product epothilone B, is the only epothilone approved for cancer therapy, indicated for metastatic breast cancer.
The epothilones competitively inhibit the binding of paclitaxel to polymerized tubulin, indicating that the two compounds share a common binding site despite signiﬁcant structural differences [90,91]. It has been reported that ixabepilone is less susceptible to drug-resistance mechanisms that limit the efficacy of taxanes, like P-glycoprotein mediated efflux and the overexpression of class III β-tubulin, due to its reduction in polymerization rate of microtubules [91,92].
Likewise taxanes, ixabepilone is also formulated in Cremophor EL yielding hypersensitivity reactions. Other side effects related to its use are neuropathy, neutropenia, severe diarrhea and fatigue [93,94].
5. Camptothecin analogs
Likewise paclitaxel, camptothecin was discovered as part of a NCI program in 1966 by Wall and Wani . Camptothecin is a pentacyclic quinoline alkaloid present in wood, bark, and fruit of the Asian tree
Top-I promote relaxation of the supercoiled DNA, prior to transcription, through the formation of a single strand break and religation. The camptothecins bind the covalent Top-I-DNA complex, known as the "cleavable complex", stabilizing it and inhibiting reannealing of the parent DNA. Consequently, camptothecins lead to reversible accumulation of double-stranded DNA breaks and tumor cell death [97-99].
Several derivatives of camptothecin have been synthesized, but only irinotecan and topotecan have been approved for clinical use. Irinotecan and topotecan, which are more soluble and less toxic analogs, are currently used in a wide spectrum of cancers. Topotecan is part of regimens to treat ovarian, lung and cervical cancer. Irinotecan is a prodrug, currently used for metastatic colorectal cancer.
Irinotecan and topotecan produces dose-limiting side effects restricting safety administration and then their anti-tumor efficacy. Diarrhea is the principal side effect related to irinotecan. Moreover the use of this drug can cause nausea, vomiting, anorexia, fatigue, abdominal pain, alopecia and neutropenia. The principal toxicity of topotecan when administered at standard doses is neutropenia, while the nonhematological toxicities are usually mild [100,101].
Podophyllotoxin was first isolated in 1880, but its structure was determined later by Hartwell and Schrecker . Despite the antineoplastic activity, podophyllotoxin was not used in clinical practice due to its toxicity. Several less toxic analogs of podophyllotoxin were produced and two analogs were approved for clinical use (etoposide and teniposide).
While etoposide is most widely used to treat lung cancer and testicular cancer, it is also effective for Hodgkin and non-Hodgkin lymphomas, acute nonlymphocytic leukemia, gastric cancer, and soft-tissue sarcomas. Teniposide has signiﬁcant activity in SCLC and in the treatment of childhood lymphomas and leukemias [103-105].
The cellular target for etoposide and teniposide is topoisomerase II (Top-II) [106,107]. Top-II enzymes regulate essential cellular processes, including DNA replication and chromosome segregation, by altering the topology of chromosomal DNA. These enzymes induce transient double-stranded breaks in the DNA allowing DNA strands to pass through each other and unwind or unknot tangled DNA. Etoposide and teniposide inhibit Top-II to religate cleaved DNA molecules . This phenomenon leads to accumulation of covalent complexes Top-II-DNA resulting in permanent DNA strand breaks, which trigger mutagenic and cell death pathways .
In addition to causing cell death, these agents may, under certain circumstances, lead to neoplastic transformation. Epipodophyllotoxin therapy can cause AML characterized by chromosomal translocations, especially in chromosome 11q23 [110,111]. Other common side effects related to antineoplastic drugs might arise, such as bone marrow suppression, nausea, vomiting and alopecia.
7.1. Anthracyclines and anthracenediones
The anthracyclines, which include doxorubicin, daunorubicin, epirubicin and idarubicin, are a class of antibiotic chemotherapeutic agents routinely used in the treatment of several cancers. While daunorubicin and idarubicin are more effective in acute leukemias, doxorubicin and epirubicin display broader activity against human solid tumors. Doxorubicin has a central role in the therapy of breast, lung, gastric, ovarian, thyroid, non-Hodgkin’s and Hodgkin’s lymphoma, sarcoma and pediatric cancers. Epirubicin is an epimer of doxorubicin indicated as component of therapy for breast cancer [112,113].
These chemotherapeutic agents attack cancer cells by multiple mechanisms (i) intercalation with DNA and disruption of Top-II, directly affecting DNA replication and repair, (ii) generation of quinone-type free radicals and their damage to cellular membranes, DNA and proteins and (iii) triggering of apoptotic cell death through complex signaling pathways [27,113].
Despite the large use, the most serious toxicity associated with anthracyclins is cardiotoxicity which can be cumulative and irreversible . However, liposomal formulation of doxorubicin was shown to be less cardiotoxic than traditional doxorubicin without compromising efficacy in adults with solid tumors .
Another important antibiotic chemotherapeutic agent is the anthracenediones, which also inhibit Top-II. Mitoxantrone is the most active compound in the anthracenediones class and has been approved for use in AML and prostate cancer [116,117]. It is relevant to point that mitoxantrone has limited ability to produce quinone-type free radicals and causes less cardiac toxicity than does doxorubicin.
Bleomycins are a group of glycopeptides antibiotics, isolated in the early 1960s from
Bleomycins are attractive components of chemotherapy regimens due to minimal myelotoxicity and immunosuppression whilst the pulmonary toxicity related to its use limits the applicability of this drug . Currently bleomycins have antitumor activity against certain types of lymphoma, testicular tumors, head and neck cancers, Kaposi sarcoma, cervical cancer and germ-cell tumors.
8.1. L-Asparaginase (L-ASNase)
L-Asparaginases (L-ASNase) are effective antineoplastic agents used in first-line treatment of a variety of lymphoproliferative disorders, especially in ALL. It has been used in combination with other agents, including methotrexate, doxorubicin, vincristine, and prednisone . Currently, there are three preparations of L-ASNase available for clinical use: native enzyme from
The mechanism of action of L-ASNase is based on the assumption that tumor cells, especially leukemic cells, require a huge amount of amino acid asparagine (Asn) to maintain their rapid malignant growth. Those cells lack adequate amounts of asparagine synthetase and are dependent on an exogenous source of Asn for survival. L-ASNase catalyzes the hydrolysis of L-ASN to L-aspartic acid and ammonia, significantly depleting the circulating asparagines from plasma [27,121].
The most common side effect is related to inhibition of protein synthesis and allergic reactions. Hypersensitivity reactions can be solved by use of modified versions of L-ASNase such as polyethylene glycol (PEG)-conjugated asparaginase (pegasparaginase). Pegasparaginase reduce immunogenic reactions and posses a considerably longer half-life, reducing the number of injections for the patient. In recent years, clinical trials have established the importance of pegasparaginase in frontline pediatric and adult ALL therapy [120,122]
Resistance arises through induction of asparagine synthetase in tumor cells  and administrations of ASNase may induce the development of antibodies that neutralize the enzyme [120, 121,124].
9. Diverse agents
The synthesis of Hydroxyurea (HU) occurred for the first time in 1869 by Dresler and Stein , meanwhile its biological activities as a myelossupressive drug were only demonstrated 60 years later . Further studies regarding its mechanism of action were able to demonstrate its activity at impairing DNA synthesis through blocking its deoxyribonucleotides subunits formation by acting at the ribonucleotide reductase (RNR) enzyme . The RNR enzyme inhibited by HU is responsible for the conversion of ribonucleotides into deoxyribonucleotides which in turn are one of the key components at the construction of DNA strands. Once HU is mainly effective at the S phase of the cell cycle, when its target, e.g. the catalytic subunit of RNR, is highly activated in cells, it also provides synergistic effect with radiotherapy . Additionally, regardless of the origin of the HU-induced release of nitric oxide [129,130], its contribution to the antineoplastic effect of HU remains relatively unexplored.
HU is currently used in combination therapies along with other chemotherapeutic agents and radiation regimens to treat resistant chronic myelocytic leukemia (CML), cervical carcinomas, malignant melanomas, head and neck cancers and brain tumors (e.g., glioma, meningioma) [127,128].
HU is well-known by its dose-limiting myelosuppressive effect which appears within a few days after the beginning of its use and is mostly reversed through the discontinuation of the drug. Skin and nail hyperpigmentation, malleolar ulcerations and solar hypersensitivity are some of the most observed cutaneous side effects in long-term exposed patients [131,132]. Multiple skin tumors as well as its precursor lesions may also develop after sun exposure [133,134].
In the late 1950s thalidomide was introduced by Chemie Grunenthal company into the market as a sedative drug and within a few years later the disseminated teratogenic consequences of its use during pregnancy practically banned its worldwide commercialization . Further studies demonstrated the antiangiogenic activity of thalidomide
The most common adverse effects associated with thalidomide’s employment include constipation and sedation. Meanwhile cardiovascular effects like hypotension and bradycardia, somnolence, thromboembolism and peripheral neuropathy are the most severe toxic events related to this drug and may require the withdrawal of it, which is generally sufficient to achieve clinical improvement [140,141].
Estramustine is a nitrogen mustard derivative formed by the union of normustine (nitrogen mustard) and estradiol-17beta-phosphate with antineoplastic effects that rely on its properties as an anti-mitotic drug through disruption of the microtubule organization in HRPC cells as well as by pro-apoptotic events . Recently concluded trials also assigned an additional benefit in treating prostate cancer with the addition of other chemotherapeutics, e.g. docetaxel, in comparison to the administration of estramustine alone .
The most common side effects observable within the use of estramustine are vomiting and nausea, affecting nearly 50% of the patients. Meanwhile the pro-estrogenic consequences (gynecomastia, impotence) and thromboembolic events are some of the most severe adverse effects. The latter, specifically is also due to the contribution of the disease itself .
Bortezomib was the first proteasome inhibitor approved by the FDA in 2003 as an alternative treatment for refractory MM. Its approval was extended in 2008 for the treatment of newly diagnosed MM. Bortezomib’s usage as an anticancer agent was also approved by the FDA in 2006 for the treatment of relapsed or refractory mantle cell lymphoma (MCL) .
This drug acts through inhibition of the 26S proteasome by blocking its 20S core subunit’s chymotrypsin-like activity, which affects several intracellular signaling pathways, as the NF-kB anti-apoptotic pathway. The NF-kB molecule is found directly attached to its inhibitor (IkB) which in turn becomes ubiquitinated and degraded at the proteasome in response to specific stressful situations, releasing NF-kB to enter the nucleus and exert its pro-survival effects. By this means, targeting the proteasome structure would lead to inhibition of NF-kB activation. Additionally, bortezomib may also promotes cancer cells to sensitization towards cytotoxic drugs .
The commonest toxic effects related to the use of bortezomib are fatigue, gastrointestinal disturbances, thrombocytopenia, paresthesia and peripheral neuropathy. Besides the adverse events aforementioned, intrinsic/acquired resistance and unsatisfactory response toward solid tumors represent some of the disadvantages associated with the utilization of bortezomib .
9.5. Zoledronic acid
Zoledronic acid is a heterocyclic nitrogen-containing bisphosphonate . Bisphosphonates, such as zoledronic acid, are anti-resorptive agents approved for treatment of skeletal complication associated with metastatic breast cancer and prostate cancer. These agents act on osteoclasts, key cells in the bone microenvironment, inhibiting bone resorption [147, 148].
Moreover, zoledronic acid is used extensively in diseases with high bone turnover such as MM. Nephrotoxicity can be observed with use of this drug, and is related to dose, infusion time and plasma concentration. Furthemore, zoledronic acid has a long renal half-life, contributing to renal damage. Osteonecrosis of the jaw is also associated with zoledronic acid [149,150].
10. Hormones and related agents
Glucocorticoids are primary stress hormones that function to maintain homeostasis regulating many biological processes, including immune function, skeletal growth, reproduction, cognition, behavior, and cell proliferation and survival . Glucocorticoids act through their binding to a specific physiological receptor that translocates to the nucleus and induces anti-proliferative and apoptotic responses in sensitive cells . These actions are important in their usage as therapeutic agents in cancer treatment.
Glucocorticoids, dexamethasone and prednisone, are widely used for the treatment of leukemias and lymphomas because of their effects on cell cycle progression and apoptosis. They are also adopted as a co-medication in the therapy of solid tumors, either because of their effectiveness in treating the malignancy or for decreasing edema, pain, electrolyte imbalance, nausea and emesis or yet to reduce cytotoxic reactions caused by other treatment regimens .
Progesterone is an essential regulator of normal human female reproductive function in the uterus, ovary, mammary gland and brain, and also plays an important role in non-reproductive tissues such as the cardiovascular system, bone and the central nervous system. This highlights the widespread role of this hormone in normal physiology. The effects of progesterone are mediated through the nuclear progesterone receptor (PR), which interacts with transcriptional coregulators, moves into nuclear aggregates and regulates gene expression .
Progestational agents, such as the agonists of the PR megestrol and megestrol acetate, have been used as second-line hormonal therapy for metastatic hormone-dependent breast cancer and in the management of endometrial carcinoma previously treated by surgery and radiotherapy .
10.3. Antiestrogens and antiandrogens
Antiestrogens and antiandrogens inhibit the binding of the natural endogenous ligands with the estrogen and androgen receptors (ER; AR) respectively. Thus they act preventing exacerbation of these receptors signaling pathways frequently observable in cancer cells. This fact lead to inhibition of cancer cells division [154, 155].
Fulvestrant is an antiestrogen approved by the FDA in 2002 for the treatment of hormone receptor positive metastatic breast cancer in post-menopausal women refractory to previous tamoxifen regimen . Fulvestrant is a complete antagonist of the ER-alfa/ER-beta and inhibits estrogen signaling by promoting mainly the degradation of ER-alfa and PRs after binding to the ER . The most observable side effects due to the usage of fulvestrant include hot flashes, thrombosis, joint disorders, pain and gastrointestinal events .
Flutamide, Bicalutamide and Nilutamide are non-steroidal antiandrogens introduced in the 1970s in order to preclude the unwanted effects caused by the nonselective profile of steroidal agents. These non-steroidal agents are only used in combination with other drugs, mostly with GnRH agonists, for the treatment of prostate cancer in order to counterbalance the effect of the released testosterone following GnRH administration. Bicalutamide has been recently approved in the European Union for the treatment of locally advanced prostate cancer and present the best schedule and adverse effects profiles. Toxic effects include hot flashes, hepatotoxicity, diarrhea, decreased libido and gynecomastia. Patients in treatment with nilulatamide may experience ocular alterations .
10.4. Selective Estrogen Receptor Modulators (SERMs)
Selective estrogen receptor modulators (SERMs) are tissue-selective compounds and depending on the site of action, exhibit agonistic (bone, liver, brain, cardiovascular system), antagonistic (brain, breast) and mixed agonist/antagonist (uterus) effects. This phenomenon occurs due to different ER subtypes expression throughout the body among other factors [159,160].
The currently SERMs approved by the FDA are tamoxifen, toremifene and raloxifen. Raloxifen is used in osteoporosis’s treatment and prevention for postmenopausal women and reduction of invasive breast cancer’s risk for women with osteoporosis or at increased risk of invasive breast cancer.
Tamoxifen is used in the treatment of metastatic breast cancer as well as in the adjuvant treatment of node-positive breast cancer. Additionally, tamoxifen demonstrates preventive effects in women at high-risk of developing breast cancer . Toremifene is used in the treatment of metastatic breast cancer in postmenopausal women with ER+ or tumors with unknown ER status .
Due to its agonistic properties, tamoxifen significantly increases the risk of endometrial cancer, pulmonary embolism and stroke, rendering the treatment based on aromatase inhibitors an interesting alternative, which demonstrate reduced frequency of the aforementioned adverse effects, though not without a high risk of loss in bone mineral density (BMD) and, consequently, fractures . Raloxifen and toremifene demonstrates similar effectiveness in comparison to tamoxifene regarding reduction of risks in developing advanced and invasive breast cancer respectively. They also show evidence of lower incidence of venous thromboembolic events and endometrial cancer [163,164].
10.5. Aromatase inhibitors
The aromatase enzyme is responsible for the conversion of androgens to estrogens and represent the primary source of estrogens in post-menopausal women. Accordingly, the aromatase inhibitors (AI) provide reduction of estrogen concentration within ER+ breast cancer cells. There are three generations of AI, which may also be classified as belonging to the type 1 (steroidal) and type 2 (non-steroidal) . Aminoglutethimide is a 1st generation nonsteroidal AI which was utilized in association to glucocorticoid in the treatment of breast cancer and is currently replaced by the following generations of AI. Formestane, a 2nd generation steroidal AI, administrated via intramuscular-injection, led to localized reactions. It also presents clinical benefits within the group of patients that experienced progressive disease after treatment with tamoxifen and nonsteroidal AI [25,165]. Exemestane is an irreversible 3rd generation orally administrated steroidal AI, which exhibits higher estrogen deprivation effect in comparison to formestane in the treatment of ER+ breast cancer progressive cases previously treated with tamoxifen. Anastrozole and letrozole are 3rd generation nonsteroidal AI which have demonstrated improved results with respect to disease free survival, recurrence rate and time to recurrence when compared to tamoxifen. This observation regards early, advanced and metastatic ER+ breast cancer treatment, irrespective of functioning as a first line adjuvant or post-tamoxifen drug. Despite the aforementioned improved clinical outcome provided by 3rd generation AIs, further long-term studies should be conducted in order to assess whether its safety profiles are superior when compared to that of tamoxifen [164,166-170).
Despite the increased number of therapeutic options, the cancer therapy remains a challenge for physicians and researchers, especially with regards to the tumor resistance. In this scenario, a better understanding about the molecular basis of cancer will enable the improvement and development of therapeutic strategies that allow an effective combat against this malignancy and better quality of life for patients.
Gilman A & Phillips FS. The Biological Actions and Therapeutic Applications of the B-Chloroethyl Amines and Sulfides. Science 1946;103(2675): 409-436.
Haskell C M. Cancer Treatment. Philadelphia: Saunders; 1990.
Arnold H, Bourseaux F, Brock N. Chemotherapeutic action of a cyclic nitrogen mustard phosphamide ester (B 518-ASTA) in experimental tumours of the rat. Nature 1958;181(4613): 931.
Friedman OM, Seligman AM. Preparation of N-phosphorylated derivatives of bis-P-chloroethylamine. Journal of the American Chemical Society 1954;76: 655-658.
Emadi A, Jones RJ, Brodsky RA. Cyclophosphamide and cancer: golden anniversary. Nature Reviews Clinical Oncology 2009;6: 638-647.
Brock N, Wilmanns H. Effect of a cyclic nitrogen mustard-phosphamidester on experimentally induced tumors in rats; chemotherapeutic effect and pharmacological properties of B518 ASTA. Deutsche Medizinische Wochenschrift 1958;83: 453-458.
Mey U, Hitz F, Lohri A, Pederiva S, Taverna C, Tzankov A, Meier O, Yeow K, Renner C. Diagnosis and treatment of diffuse large B-cell lymphoma. Swiss Medical Weekly 2012; 142:0.
Hillmen P. Using the biology of chronic lymphocytic leukemia to choose treatment. American Society of Hematology Education Program 2011;2011: 104-109.
López-Tarruella S, Martín M. Recent advances in systemic therapy: advances in adjuvant systemic chemotherapy of early breast cancer. Breast Cancer Research 2009;11: 204.
Clowse ME, Behera MA, Anders CK, Copland S, Coffman CJ, Leppert PC, Bastian LA. Ovarian preservation by GnRH agonists during chemotherapy: a meta-analysis. Journal of Womens Health (Larchmt) 2009;18: 311-319.
Siddik ZH. Mechanism of action of cancer chemotherapeutic agents: DNA-interactive alkylating agents and antitumour platinum-based drugs. In The cancer handbook. Edited by Alison M R. New Jersey: John Wiley & Sons 2002.
Colvin ME, Sasaki JC, Tran NL. Chemical factors in the action of phosphoramidic mustard alkylating anticancer drugs: roles for computational chemistry. Current Pharmaceutical Design 1999;5: 645-663.
Corsi A, Calabresi F, Greco C. Comparative effects of cyclophosphamide and isophosphamide on Lewis lung carcinoma. British Journal of Cancer 1978;38: 631-633.
Penel N, Van Glabbeke M, Marreaud S, Ouali M, Blay JY, Hohenberger P. Testing new regimens in patients with advanced soft tissue sarcoma: analysis of publications from the last 10 years. Annals of Oncology 2011;22: 1266-1272.
Voss MH, Feldman DR, Bosl GJ, Motzer RJ. A review of second-line chemotherapy and prognostic models for disseminated germ cell tumors. Hematology/Oncology Clinics of North America 2011;25: 557-576.
Wasserman TH, Slavik M, Carter SK. Clinical comparison of the nitrosoureas. Cancer 1975;36: 1258-1268.
Marchesi F, Turriziani M, Tortorelli G, Avvisati G, Torino F, De Vecchis L. Triazene compounds: mechanism of action and related DNA repair systems. Pharmacological Research 2007;56: 275-287.
Neyns B, Tosoni A, Hwu WJ, Reardon DA. Dose-dense temozolomide regimens: antitumor activity, toxicity, and immunomodulatory effects. Cancer 2010;116(12): 2868-2877.
Advani R. Optimal therapy of advanced Hodgkin lymphoma. American Society of Hematology Education Program 2011;2011: 310-316.
Flaherty KT. Chemotherapy and targeted therapy combinations in advanced melanoma. Clinical Cancer Research 2006;12: 2366-2370.
Rosenberg B, Vancamp L, Krigas T. Inhibition of cell division in Escherichia coli by electrolysis products from a platinum electrode. Nature 1965;205: 698-699.
Desoize B. Metals and metal compounds in cancer treatment. Anticancer Research 2004;24(3a): 1529-1544.
Boulikas T, Pantos A, Bellis E, Christofis P. Designing platinum compounds in cancer: structures and mechanisms. Cancer Therapy 2007;5: 537-583.
Gore ME, Fryatt I, Wiltshaw E, Dawson T, Robinson BA & Calvert AH. Cisplatin/carboplatin cross-resistance in ovarian cancer. British Journal of Cancer 1989;60: 767-769.
Hong WK, Bast Jr RC, Hait WN, Kufe DW, Pollock RE, Weichselbaum RR, Holland JF & Frei III E. Cancer Medicine 8. American Association for Cancer Research 2010.
Saris CP, van de Vaart PJ, Rietbroek RC, Blommaert FA. In vitro formation of DNA adducts by cisplatin, lobaplatin and oxaliplatin in calf thymus DNA in solution and in cultured human cells. Carcinogenesis 1996;17(12): 2763-2769.
Brunton LL, Chabner BA & Knollmann BC. Goodman & Gilman’s. The Pharmacological Basis of Terapeutics. New York: McGraw-Hill Companies 2011.
Pasetto LM, D'Andrea MR, Brandes AA, Rossi E & Monfardini S. The development of platinum compounds and their possible combination. Critical Reviews in Oncology Hematology 2006;60: 59-75.
Farber S, Diamond LK. Temporary remissions in acute leukemia in children produced by folic acid antagonist, 4-aminopteroyl-glutamic acid. New England Journal of Medicine. 1948; 238(23):787-793.
Zhao R & Goldman ID. Resistance to antifolates. Oncogene 2003;22(47): 7431-7457.
Chabner BA, Allegra CJ, Curt GA, Clendeninn NJ, Baram J, Koizumi S, Drake JC, Jolivet J. Polyglutamation of methotrexate. Is methotrexate a prodrug? The Journal of Clinical Investigation 1985;76(3): 907-912.
Cho RC, Cole PD, Sohn KJ, Gaisano G, Croxford R, Kamen BA, Kim YI. Effects of folate and folylpolyglutamyl synthase modulation on chemosensitivity of breast cancer cells. Molecular Cancer Therapy 2007;6(11): 2909-2920.
Fabre I, Fabre G, Goldman ID. Polyglutamylation, an important element in methotrexate cytotoxicity and selectivity in tumor versus murine granulocytic progenitor cells in vitro. Cancer Research. 1984;44(8): 3190-3195.
Holmboe L, Andersen AM, Mørkrid L, Slørdal L, Hall KS. High dose methotrexate chemotherapy: pharmacokinetics, folate and toxicity in osteosarcoma patients. British Journal of Clinical Pharmacology 2012;73(1): 106-114.
Salliot C & Van der Heijde D. Long-term safety of methotrexate monotherapy in patients with rheumatoid arthritis: a systematic literature research. Annals of the Rheumatic Diseases 2009;68(7): 1100-1104.
Buqué A, Muhialdin JS, Muñoz A, Calvo B, Carrera S, Aresti U, Sancho A, Rubio I, López-Vivanco G. Molecular mechanism implicated in Pemetrexed induced apoptosis in human melanoma cells. Molecular Cancer 2012;11(1): 25.
Scagliotti GV, Parikh P, von Pawel J, Biesma B, Vansteenkiste J, Manegold C, Serwatowski P, Gatzemeier U, Digumarti R, Zukin M, Lee JS, Mellemgaard A, Park K, Patil S, Rolski J, Goksel T, de Marinis F, Simms L, Sugarman KP, Gandara D. Phase III study comparing cisplatin plus gemcitabine with cisplatin plus pemetrexed in chemotherapy-naive patients with advanced-stage non-small-cell lung cancer. Journal of Clinical Oncology 2008;26(21): 3543-3551.
Adjei AA. Pharmacology and mechanism of action of pemetrexed. Clinical Lung Cancer 2004;5Suppl2: S51-55.
Hitchings GH Jr. Nobel lecture in physiology or medicine - 1988. Selective inhibitors of dihydrofolate reductase. In Vitro Cellular Developmental Biology 1989;25(4): 303-310.
Galmarini CM, Mackey JR, Dumontet C. Nucleoside analogues and nucleobases in cancer treatment. The Lancet Oncology 2002;3(7): 415-424.
Stanczyk M, Sliwinski T, Trelinska J, Cuchra M, Markiewicz L, Dziki L, Bieniek A, Bielecka-Kowalska A, Kowalski M, Pastorczak A, Szemraj J, Mlynarski W, Majsterek I. Role of base-excision repair in the treatment of childhood acute lymphoblastic leukaemia with 6-mercaptopurine and high doses of methotrexate. Mutation Research 2012;741(1-2): 13-21.
Dervieux T, Blanco JG, Krynetski EY, Vanin EF, Roussel MF, Relling MV. Differing contribution of thiopurine methyltransferase to mercaptopurine versus thioguanine effects in human leukemic cells. Cancer Research 2001;61(15): 5810-5816
Elion GB. Nobel lecture in physiology or medicine - 1988. The purine path to chemotherapy. In Vitro Cell Development Biology 1989;25(4): 321-330.
Karran P & Attard N. Thiopurines in current medical practice: molecular mechanisms and contributions to therapy-related cancer. Nature Review Cancer 2008;8(1): 24-36.
Plunkett W, Huang P, Gandhi V. Metabolism and action of fludarabine phosphate. Seminars Oncology 1990;17(5): 3-17.
Chun HG, Leyland-Jones B, Cheson BD. Fludarabine phosphate: a synthetic purine antimetabolite with significant activity against lymphoid malignancies. Journal of Clinical Oncology 1991;9(1): 175-188.
Yang SW, Huang P, Plunkett W, Becker FF, Chan JY. Dual mode of inhibition of purified DNA ligase I from human cells by 9-beta-D-arabinofuranosyl-2-fluoroadenine triphosphate. The Journal of Biological Chemistry 1992;267(4): 2345-2349.
Catapano CV, Perrino FW, Fernandes DJ. Primer RNA chain termination induced by 9-beta-D-arabinofuranosyl-2-fluoroadenine 5'-triphosphate. A mechanism of DNA synthesis inhibition. Journal of Biological Chemistry 1993;268(10): 7179-7185.
Cheson BD, Vena DA, Foss FM, Sorensen JM. Neurotoxicity of purine analogs: a review. Journal of Clinical Oncology 1994;12(10): 2216-2228.
Beutler E. Cladribine (2-chlorodeoxyadenosine). Lancet 1992;340(8825): 952-956.
Klohs WD & Kraker AJ. Pentostatin: future directions. Pharmacological Reviews 1992;44(4): 459-477.
Longley DB, Harkin DP, Johnston PG. 5-Fluorouracil: Mechanisms of action and clinical strategies. Nature 2003;3: 330-338.
Lamont EB & Schilsky RL. The Oral Fluoropyrimidines in Cancer Chemotherapy. Clinical Cancer Research 1999;5: 2289-2296.
Han R, Yang YM, Dietrich J, Luebke A, Mayer-Pröschel M, Noble M. Systemic 5-fluorouracil treatment causes a syndrome of delayed myelin destruction in the central nervous system. Journal of Biology 2008;7(4): 12.
Stewart T, Pavlakis N, Ward M. Cardiotoxicity with 5-fluorouracil and capecitabine: more than just vasospastic angina. Internal Medicine Journal 2010;40(4): 303-307.
Miwa M, Ura M, Nishida M, Sawada N, Ishikawa T, Mori K, Shimma N, Umeda I, Ishitsuka H. Design of a novel oral fluoropyrimidine carbamate, capecitabine, which generates 5-fluorouracil selectively in tumours by enzymes concentrated in human liver and cancer tissue. European Journal of Cancer 1998;34(8): 1274–1281.
Johnston PG & Kaye S. Capecitabine: a novel agent for the treatment of solid tumors. Anticancer Drugs 2001;12(8): 639-646.
Qiu MT, Ding XX, Hu JW, Tian HY, Yin R, Xu L. Fixed-dose rate infusion and standard rate infusion of gemcitabine in patients with advanced non-small-cell lung cancer: a meta-analysis of six trials. Cancer Chemotherapy and Pharmacology 2012; DOI: 10.1007/s00280-012-1974-z.
Hui YF & Reitz J. Gemcitabine: A cytidine analogue active against solid tumors. American Journal of Health-System Pharmacy 1997;54: 162-170.
Kim KI, Huh IS, Kim IW, Park T, Ahn KS, Yoon SS, Yoon JH, Oh JM. Combined interaction of multi-locus genetic polymorphisms in cytarabine arabinoside metabolic pathway on clinical outcomes in adult acute myeloid leukaemia (AML) patients. European Journal of Cancer 2012;DOI: 10.1016/j.ejca.2012.07.022.
Hartford CM, Duan S, Delaney SM, Mi S, Kistner EO, Lamba JK, Huang RS, Dolan ME. Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity. Blood 2009;113(10): 2145–2153.
Johnson IS, Wright HF, Svoboda GH, Vlantis J. Antitumor principles derived from Vinca rosea Linn. I. Vincaleukoblastine and leurosine. Cancer Research 1960;20: 1016-1022.
Jordan MA, Thrower D, Wilson L. Mechanism of inhibition of cell proliferation by vinca alkaloids. Cancer Research 1991;51(8): 2212-2222.
Jordan MA & Wilson L. Microtubules as a target for anticancer drugs. Nature Reviews Cancer 2004;4: 253-265.
Dumontet C & Jordan MA. Microtubule-binding agents: a dynamic field of cancer therapeutics. Nature Reviews Drug Discovery 2010;9(10): 790-803.
Kingston DG. Tubulin-interactive natural products as anticancer agents. Journal Natural Products 2009;72: 507-515.
Lucas DM, Still PC, Pérez LB, Grever MR & Kinghorn AD. Potential of Plant-Derived Natural Products in the Treatment of Leukemia and Lymphoma. Current Drug Targets 2010;11: 812–822.
Groninger E, Meeuwsen-de Boar T, Koopmans P, Uges D, Sluiter W, Veerman A, Kamps W, de Graaf S. Pharmacokinetics of vincristine monotherapy in childhood acute lymphoblastic leukemia. Pediatric Research 2002;52(1): 113-118.
Rowinsky EK & Donehower RC. The clinical pharmacology and use of antimicrotubule agents in cancer chemotherapeutics. Pharmacology & Therapeutics 1991;52: 35-84.
Mano M. Vinorelbine in the management of breast cancer: New perspectives, revived role in the era of targeted therapy. Cancer Treatment Reviews 2006;32: 106-118
Gralla RJ, Gatzemeier U, Gebbia V, Huber R, O'Brien M, & Puozzo C.Oral vinorelbine in the treatment of non-small cell lung cancer: rationale and implications for patient management. Drugs 2007;67: 1403-1410.
Quasthoff S & Hartung HP. Chemotherapy-induced peripheral neuropathy. Journal of Neurology 2002;249(1): 9-17.
Canta A, Chiorazzi A, Cavaletti G. Tubulin: a target for antineoplastic drugs into the cancer cells but also in the peripheral nervous system. Current Medicinal Chemistry 2009;16(11): 1315-1324.
Goa KL & Faulds D. Vinorelbine. A review of its pharmacological properties and clinical use in cancer chemotherapy. Drugs Aging 1994;5(3): 200-234.
Gottesman MM, Fojo T, Bates SE. Multidrug resistance in cancer: role of ATP-dependent transporters. Nature Reviews Cancer 2002;2(1): 48-58.
Natarajan K, Senapati S. Understanding the basis of drug resistance of the mutants of αβ-tubulin dimer via molecular dynamics simulations. PLoS One 2012;7(8):e42351 1-13.
Gonzalez-Garay ML, Chang L, Blade K, Menick DR, Cabral F.A beta-tubulin leucine cluster involved in microtubule assembly and paclitaxel resistance. The Journal of Biological Chemistry 1999;274(34): 23875-23882.
Burkhart CA, Kavallaris M, Band Horwitz S. The role of beta-tubulin isotypes in resistance to antimitotic drugs. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics 2001;1471(2): O1-9.
Zhang CC, Yang JM, White E, Murphy M, Levine A, Hait WN. The role of MAP4 expression in the sensitivity to paclitaxel and resistance to vinca alkaloids in p53 mutant cells. Oncogene 1998;16(12): 1617-1624.
Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT. Plant antitumor agents VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. Journal of the American Chemical Society 1971;93(9): 2325-2327.
Wall ME, Wani MC. Camptothecin and taxol: discovery to clinic--thirteenth Bruce F. Cain Memorial Award Lecture. Cancer Research 1995;55(4): 753-760.
Guenard D, Gueritte-Voegelein F, Potier P. Taxol and taxotere: discovery, chemistry, and structure-activity relationships. Accounts of Chemical Research 1993;26(4): 160–167.
Rowinsky EK. The development and clinical utility of the taxane class of antimicrotubule chemotherapy agents. Annual Reviews Medicine 1997;48: 353-374.
Horwitz SB, Cohen D, Rao S, et al. Taxol: mechanisms of action and resistance. Journal of the National Cancer Institute 1994;15: 63–67.
Haldar S, Chintapalli J, Croce CM. Taxol induces bcl-2 phosphorylation and death of prostate cancer cells. Cancer Research 1996; 56: 1253–1255.
Weiss RB, Donehower RC, Wiernik PH, Ohnuma T, Gralla RJ, Trump DL, Baker JR Jr, Van Echo DA, Von Hoff DD, Leyland-Jones B. Hypersensitivity reactions from taxol. Journal of Clinical Oncology 1990;8(7): 1263-1268.
Rowinsky EK, Donehower RC (1995) Paclitaxel (taxol). New England Journal of Medicine 1995;332: 1004–1014
Miele E, Spinelli GP, Miele E, Tomao F, Tomao S. Albumin-bound formulation of paclitaxel (Abraxane ABI-007) in the treatment of breast cancer. International Journal of Nanomedicine 2009;4: 99-105.
Rowinsky EK, Eisenhauer EA, Chaudhry V, Arbuck SG, Donehower RC. Clinical toxicities encountered with paclitaxel (Taxol). Seminars in Oncology 1993;20(Suppl 3): 1-15.
Cortes J & Baselga J. Targeting the microtubules in breast cancer beyond taxanes: the epothilones. Oncologist 2007;12: 271-280.
Nettles JH, Li H, Cornett B, Krahn JM, Snyder JP, Downing KH. The binding mode of epothilone A on α,β-tubulin by electron crystallography. Science 2004;305: 866–869
Bode CJ, Gupta ML Jr, Reiff EA, Suprenant KA, Georg GI & Himes RH. Epothilone and paclitaxel: unexpected differences in promoting the assembly and stabilization of yeast microtubules. Biochemistry 2002;41: 3870–3874.
de Jonge M & Verweij J. The epothilone dilemma. Journal of Clinical Oncology 2005;23(36): 9048-9050.
Goodin S, Kane MP, Rubin EH. Epothilones: mechanism of action and biologic activity. Journal of Clinical Oncology 2004;22(10): 2015-2025.
Wall ME, Wani MC, Cook CE, Palmer KH. Plant antitumor agents, I: the isolation and structure of camptothecin, a novel alkaloidal leukemia and tumor inhibitor from Camptotheca acuminate. Journal of the American Chemical Society 1966;88: 3888–3890
Pommier Y. Topoisomerase I inhibitors: camptothecins and beyond. Nature Reviews Cancer 2006;6(10): 789-802.
Hsiang YH, Hertzberg R, Hecht S, Liu LF. Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. The Journal of Biological Chemistry 1985;260(27): 14873–14878.
Eng WK, Faucette L, Johnson RK, Sternglanz R. Evidence that DNA topoisomerase I is necessary for the cytotoxic effects of camptothecin. Molecular Pharmacology 1988;34(6): 755–760.
Jaxel C, Kohn KW, Wani MC, Wall ME, Pommier Y. Structure-activity study of the actions of camptothecin derivatives on mammalian topoisomerase I: evidence for a specific receptor site and a relation to antitumor activity. Cancer Research 1989;49(6): 1465–1469.
Grochow LB, Rowinsky EK, Johnson R, Ludeman S, Kaufmann SH, McCabe FL, Smith BR, Hurowitz L, DeLisa A, Donehower RC, et al. Pharmacokinetics and pharmacodynamics of topotecan in patients with advanced cancer. Drug Metabolism and Disposition 1992;20(5): 706-713.
Ormrod D, Spencer CM. Topotecan: a review of its efficacy in small cell lung cancer. Drugs. 1999;58(3): 533-551.
Hartwell JL & Schrecker AW. Components of Podophyllin. V. The Constitution of Podophyllotoxin. Journal of the American Chemical Society 1951;73(6): 2909–2916.
Lucas DM, Still PC, Pérez LB, Grever MR, Kinghorn AD. Potential of plant-derived natural products in the treatment of leukemia and lymphoma. Current Drug Targets 2010;11(7): 812-822.
Hande KR. Topoisomerase II inhibitors. Update on Cancer Therapeutics 2006;1: 3-15.
Hande KR. Etoposide: four decades of development of a topoisomerase II inhibitor. European Journal of Cancer 1998;34: 1514-1521.
Long BH, Casazza AM. Structure-activity relationships of VP-16 analogues. Cancer Chemotherapy Pharmacology 1994;34: 26-31.
Ross W, Rowe T, Glisson B, Yalowich J, Liu L. Role of topoisomerase II in mediating epipodophyllotoxin-induced DNA cleavage. Cancer Research 1984;44: 5857–5860.
Osheroff N. Effect of antineoplastic agents on the DNA cleavage/religation reaction of eukaryotic topoisomerase II: inhibition of DNA religation by etoposide. Biochemistry 1989;28: 6157–6160.
Fan JR, Peng AL, Chen HC, Lo SC, Huang TH, Li TK. Cellular processing pathways contribute to the activation of etoposide-induced DNA damage responses. DNA Repair 2008;7(3): 452-463.
Blanco JG, Dervieux T, Edick MJ, Mehta PK, Rubnitz JE, Shurtleff S, Raimondi SC, Behm FG, Pui CH, Relling MV. Molecular emergence of acute myeloid leukemia during treatment for acute lymphoblastic leukemia. Proceedings of National Acadamy of Sciences of the United States of America 2001;98(18): 10338-10343.
Ratain MJ, Rowley JD. Therapy-related acute myeloid leukemia secondary to inhibitors of topoisomerase II: from the bedside to the target genes. Annals of Oncology 1992;3(2): 107-111.
Cortés-Funes H & Coronado C. Role of anthracyclines in the era of targeted therapy. Cardiovascular Toxicology 2007;7: 56-60.
Thorn CF, Oshiro C, Marsh S, Hernandez-Boussard T, McLeod H, Klein TE & Altman RB. Doxorubicin pathways: pharmacodynamics and adverse effects. Pharmacogenetics and Genomics 2011;21: 440-446.
Pai VB, Nahata MC. Cardiotoxicity of chemotherapeutic agents: incidence, treatment and prevention. Drug Safety 2000;22(4): 263-302.
Batist G, Ramakrishnan G, Rao CS, Chandrasekharan A, Gutheil J, Guthrie T, Shah P, Khojasteh A, Nair MK, Hoelzer K, Tkaczuk K, Park YC, Lee LW. Reduced cardiotoxicity and preserved antitumour efﬁcacy of liposome-encapsulated doxorubicin and cyclophosphamide compared with conventional doxorubicin and cyclophosphamide in a randomized, multicenter trial of metastatic breast cancer. Journal of Clinical Oncology 2001;19: 1444–1454.
Trifilio SM, Rademaker AW, Newman D, Coyle K, Carlson-Leuer K, Mehta J, Altman J, Frankfurt O, Tallman MS. Mitoxantrone and etoposide with or without intermediate dose cytarabine for the treatment of primary induction failure or relapsed acute myeloid leukemia. Leukemia Research 2012;36: 394-396.
Doggrell SA. Which drug combination for hormone-refractory prostate cancer?. Expert Opinion on Pharmacotherapy 2005;6: 667-670.
Umezawa H. Natural and artiﬁcial bleomycins: chemistry and antitumor activities. Pure and Applied Chemistry 1971;28: 665–680.
Chen J & Stubbe J. Bleomycins: towards better therapeutics. Nature Reviews Cancer 2005;5: 102-112.
Masetti R & Pession A. First-line treatment of acute lymphoblastic leukemia with pegasparaginase. Biologics 2009;3: 359-368.
Avramis VI. Asparaginases: A Successful Class of Drugs Against Leukemias and Lymphomas. Journal of Pediatric Hematology/Oncology 2011;33: 573-579
Dinndorf PA, Gootenberg J, Cohen MH, Keegan P, Pazdur R. FDA drug approval summary: pegaspargase (oncaspar) for the first-line treatment of children with acute lymphoblastic leukemia (ALL). The Oncologist 2007;12(8): 991-998.
Hutson RG, Kitoh T, Moraga Amador DA, Cosic S, Schuster SM, Kilberg MS.Amino acid control of asparagine synthetase: relation to asparaginase resistance in human leukemia cells. The American Journal of Physiology 1997;272(5): 1691-1699.
Albertsen BK, Schroder H, Jakobsen P, et al. Antibody formation during intravenous and intramuscular therapy with Erwinia asparaginase. Medical and Pediatric Oncology 2002;38: 310–316
Dresler WFC, Stein R. Uber den Hydroxylharnstoff. Justus Liebigs Annalen der Chemie 1869;150(2): 242-252.
Rosenthal F, Wislicki L, Koller L. Uber die Bziehungen von schwertsen Blutgiften zu Abauprodukten des Einweisses: ein Beitrag zum Enstehungmechanismus der pernizosen Anemie. Klin Wochenschr 1928;7: 972-977.
Saban N, Bujak M. Hydroxyurea and hydroxamic acid derivatives as antitumor drugs. Cancer Chemotherapy and Pharmacology 2009;64(2): 213-221.
Madaan K, Kaushik D, Verma T. Hydroxyurea: a key player in cancer chemotherapy. Expert Review of Anticancer Therapy 2012;12(1): 19-29.
Cokic VP, Smith RD, Beleslin-Cokic BB, Njoroge JM, Miller JL, Gladwin MT, Schechter AN. Hydroxyurea induces fetal hemoglobin by the nitric oxide-dependent activation of soluble guanylyl cyclase. Journal of Clinical Investigation 2003;111(2): 231-239.
Huang J, Kim-Shapiro DB, King SB. Catalase-mediated nitric oxide formation from hydroxyurea. Journal of Medicinal Chemistry 2004;47(14): 3495-3501.
Kennedy BJ, Smith LR, Goltz RW. Skin changes secondary to hydroxyurea therapy. Archives of Dermatology 1975;111(2): 183-187.
Friedrich S, Raff K, Landthaler M, Karrer S. Cutaneous ulcerations on hands and heels secondary to long-term hydroxyurea treatment. European Journal of Dermatology 2004;14(5): 343-346.
Best PJ, Petitt RM. Multiple skin cancers associated with hydroxyurea therapy. Mayo Clinic Proceedings: Mayo Clinic 1998;73(10): 961-963.
Sanchez-Palacios C, Guitart J. Hydroxyurea-associated squamous dysplasia. Journal of the American Academy of Dermatology 2004;51(2): 293-300.
Matthews SJ, McCoy C. Thalidomide: a review of approved and investigational uses. Clinical Therapeutics 2003;25(2): 342-395.
Bartlett JB, Dredge K, Dalgleish AG. The evolution of thalidomide and its IMiD derivatives as anticancer agents. Nature Reviews Cancer 2004;4(4): 314-322.
FDA - U.S. Food and Drug Administration. FDA: Thalidomide (marketed as Thalomid) Information. www.fda.gov/Drugs/DrugSafety/PostmarketDrugSafetyInformationforPatientsandProviders/ucm107296.htm (accessed 01 August 2012)
Bruera E, Neumann CM, Pituskin E, Calder K, Ball G, Hanson J. Thalidomide in patients with cachexia due to terminal cancer: preliminary report. Annals of Oncology 1999;10(7): 857-859.
. Gordon JN, Trebble TM, Ellis RD, Duncan HD, Johns T, Goggin PM. Thalidomide in the treatment of cancer cachexia: a randomised placebo controlled trial. Gut 2005; 54(4): 540-545.
. Grover JK, Uppal G, Raina V. The adverse effects of thalidomide in relapsed and refractory patients of multiple myeloma. Annals of Oncology 2002;13(10): 1636-1640.
Dimopoulos MA, Eleutherakis-Papaiakovou V. Adverse effects of thalidomide administration in patients with neoplastic diseases. American Journal of Medicine 2004;117(7): 508-515.
Mohan R, Panda D. Kinetic stabilization of microtubule dynamics by estramustine is associated with tubulin acetylation, spindle abnormalities, and mitotic arrest. Cancer Research 2008;68(15): 6181-6189.
Ravery V, Fizazi K, Oudard S, Drouet L, Eymard JC, Culine S, Gravis G, Hennequin C, Zerbib M. The use of estramustine phosphate in the modern management of advanced prostate cancer. BJU International 2011;108(11): 1782-1786.
U.S. Food and Drug administration. FDA: FDA approves bortezomib (Velcade) for the treatment of patients with mantle cell lymphoma who have received at least one prior therapy. www.fda.gov/AboutFDA/CentersOffices/OfficeofMedicalProductsandTobacco/CDER/ucm094929.htm (accessed 01 August 2012)
Chen D, Frezza M, Schmitt S, Kanwar J, Dou QP. Bortezomib as the first proteasome inhibitor anticancer drug: current status and future perspectives. Current Cancer Drug Targets 2011;11(3): 239-253.
Major P. The use of zoledronic acid, a novel, highly potent bisphosphonate, for the treatment of hypercalcemia of malignancy. The Oncologist 2002;7: 481-491.
Green JR. Antitumor effects of bisphosphonates. Cancer 2003;97(3 Suppl): 840-847.
Rogers TL & Holen I. Tumour macrophages as potential targets of bisphosphonates. Journal of Translational Medicine 2011;179: 177.
Pozzi S & Raje N. The role of bisphosphonates in multiple myeloma: mechanisms, side effects, and the future. The Oncologist 2011;16: 651-662.
Richardson PG, Laubach JP, Schlossman RL, Ghobrial IM, Mitsiades CS, Rosenblatt J, Mahindra A, Raje N, Munshi N & Anderson KC.The Medical Research Council Myeloma IX trial: the impact on treatment paradigms. European Journal of Haematology. 2012;88: 1-7.
Oakley RH & Cidlowski JA. Cellular processing of the glucocorticoid receptor gene and protein: new mechanisms for generating tissue-specific actions of glucocorticoids. The Journal of Biological Chemistry 2011;286: 3177-3184.
Schlossmacher G, Stevens A & White A. Glucocorticoid receptor-mediated apoptosis: mechanisms of resistance in cancer cells. Journal of Endocrinology 2011;211: 17-25.
Scarpin KM, Graham JD, Mote PA & Clarke CL. Progesterone action in human tissues: regulation by progesterone receptor (PR) isoform expression, nuclear positioning and coregulator expression. Nuclear Receptor Signaling 2009;7: e009. www.nursa.org/article.cfm?doi=10.1621/nrs.07009 (accessed 20 October 2012).
Heldring N, Pike A, Andersson S, Matthews J, Cheng G, Hartman J, Tujague M, Ström A, Treuter E, Warner M, Gustafsson JA. Estrogen receptors: how do they signal and what are their targets. Physiological Reviews 2007;87(3): 905-931.
Chen Y, Clegg NJ, Scher HI. Anti-androgens and androgen-depleting therapies in prostate cancer: new agents for an established target. Lancet Oncology 2009;10(10): 981-991.
U.S. Food and Drug administration. FDA: Drug Databases: Drugs@FDA: Fulvestrant Label and Approval History: Labeling Revision (11/09/2012). www.accessdata.fda.gov/drugsatfda_docs/label/2012/021344s019s020lbl.pdf. (accessed 20 November 2012)
Robertson JF. Fulvestrant (Faslodex) -- how to make a good drug better. Oncologist 2007;12(7): 774-784.
Valachis A, Mauri D, Polyzos NP, Mavroudis D, Georgoulias V, Casazza G. Fulvestrant in the treatment of advanced breast cancer: a systematic review and meta-analysis of randomized controlled trials. Critical Reviews in Oncology/Hematolology 2010;73(3): 220-227.
Lewis JS, Jordan VC. Selective estrogen receptor modulators (SERMs): mechanisms of anticarcinogenesis and drug resistance. Mutation Research 2005;591(1-2): 247-263.
Pickar JH, MacNeil T, Ohleth K. SERMs: progress and future perspectives. Maturitas 2010;67(2): 129-138.
U.S. Food and Drug administration. FDA: Drug Databases: Drugs@FDA: Nolvadex Label and Approval History: Labeling Revision (03/17/2005). www.accessdata.fda.gov/drugsatfda_docs/label/2005/17970s053lbl.pdf. (accessed 20 November 2012)
U.S. Food and Drug administration. FDA: Drug Databases: Drugs@FDA: Fareston Label and Approval History: Labeling Revision (03/21/2011). www.accessdata.fda.gov/drugsatfda_docs/label/2011/020497s006lbl.pdf. (accessed 20 November 2012)
Vogel VG, Costantino JP, Wickerham DL, Cronin WM, Cecchini RS, Atkins JN, Bevers TB, Fehrenbacher L, Pajon ER Jr, Wade JL 3rd, Robidoux A, Margolese RG, James J, Lippman SM, Runowicz CD, Ganz PA, Reis SE, McCaskill-Stevens W, Ford LG, Jordan VC, Wolmark N; National Surgical Adjuvant Breast and Bowel Project (NSABP). Effects of tamoxifen vs raloxifene on the risk of developing invasive breast cancer and other disease outcomes: the NSABP Study of Tamoxifen and Raloxifene (STAR) P-2 trial. JAMA 2006;295(23): 2727-2741.
Cuzick J, Sestak I, Baum M, Buzdar A, Howell A, Dowsett M, Forbes JF; ATAC/LATTE investigators. Effect of anastrozole and tamoxifen as adjuvant treatment for early-stage breast cancer: 10-year analysis of the ATAC trial. Lancet Oncology 2010;11(12): 1135-1341
Johnston SR, Dowsett M. Aromatase inhibitors for breast cancer: lessons from the laboratory. Nature Reviews Cancer 2003;3(11): 821-831.
Dowsett M, Cuzick J, Ingle J, Coates A, Forbes J, Bliss J, Buyse M, Baum M, Buzdar A, Colleoni M, Coombes C, Snowdon C, Gnant M, Jakesz R, Kaufmann M, Boccardo F, Godwin J, Davies C, Peto R. Meta-analysis of breast cancer outcomes in adjuvant trials of aromatase inhibitors versus tamoxifen. Journal of Clinical Oncology 2010;28(3): 509-518
Huiart L, Dell'Aniello S, Suissa S. Use of tamoxifen and aromatase inhibitors in a large population-based cohort of women with breast cancer. British Journal of Cancer 2011;104(10): 1558-1563.
Jakesz R, Jonat W, Gnant M, Mittlboeck M, Greil R, Tausch C, Hilfrich J, Kwasny W, Menzel C, Samonigg H, Seifert M, Gademann G, Kaufmann M, Wolfgang J; ABCSG and the GABG. Switching of postmenopausal women with endocrine-responsive early breast cancer to anastrozole after 2 years' adjuvant tamoxifen: combined results of ABCSG trial 8 and ARNO 95 trial. Lancet 2005;366(9484): 455-462.
Regan MM, Neven P, Giobbie-Hurder A, Goldhirsch A, Ejlertsen B, Mauriac L, Forbes JF, Smith I, Láng I, Wardley A, Rabaglio M, Price KN, Gelber RD, Coates AS, Thürlimann B; BIG 1-98 Collaborative Group; International Breast Cancer Study Group (IBCSG). Assessment of letrozole and tamoxifen alone and in sequence for postmenopausal women with steroid hormone receptor-positive breast cancer: the BIG 1-98 randomised clinical trial at 8-1 years median follow-up. Lancet Oncology 2011;12(12): 1101-1108.